Carbohydrate Allocation Patterns in Southern Minnesota

Herbicide (endothal) treated Curlyleaf Pondweed

(Potomogeton crispus) Populations

Erika Magnusson, Zach Gutknecht and Dr. Christopher Ruhland, PhD

Biology DepartmentMinnesota State University, Mankato

PlantWise et al 2013Frank Koshere et al. nd.

•Curlyleaf pondweed is the most widely-dispersed nuisance-forming non-native submersed aquatic plant in the state of Minnesota.

•Native to Europe and Asia, curlyleaf pondweed is now thoroughly naturalized in North America.

P. crispus Turion Biology• Ridged organs that provide:

– Protection against frost– Store carbohydrates used for germination– Can photosynthesize

Peterson, M. et al 2003Crow Wing Lakes Association et al. 2010

P. crispus Biology

•Woolf, T. & Madsen, J. 2003.

• Ideal time to initiate management and control efforts

• Carbohydrate Low Points

• Carbohydrate utilization from storage exceeds carbohydrate production

Plant Life Cycle Vulnerable Periods

My research is attempting to assess if endothall can effectively control P. crispus turions by decreasing carbohydrate concentrations.

Purpose of Study

1. To analyze the effect of endothal on turion viability by measuring turion Total Nonstructural Carbohydrate concentrations (g/ m^2)

1. Analyze the difference: • Between treated and untreated areas within

Duck Lake and untreated areas of West Jefferson Lake, and Lake Ballantyne

Goals of This Study

Ballantyne Lake

Duck Lake

West Jefferson Lake

Study Lakes

“Colorimetric Methods for Determination of Sugars” previously cited by Michel Dubois, K.A. Gilles, J.K.

Hamilton, P.A. Rebers and Fred Smith (1955).

• West Jefferson • Duck Lake treated• Duck Lake untreated• Lake Ballantyne

Procedure

Method Procedure

1. Extraction: Ethanol (EtOH) method

2. Starch Digestion: a amlyase- amyloglucosidase method

3. Sugar analysis with a peroxidase-glucose oxidase/odianisidine reagent (PGO)

• One-way Anova and the Post-Hoc is a Dunn’s method

Results

Conclusions• It is possible that endothal is affective at decreasing Curlyleaf

Pondweed turion TNC • Future Research: Endothal affect on Curlyleaf Pondweed turion

itself and not just the plant portion. – Noticed that turions collected had less ridigity (were softer) in Duck

Lake treated areas than DU, WJ and BL– Does endothal prevent nutrient sources from forming in Turions?

Bibliography• Crow Wing Lakes Association. (2010, April 29). Curly Leaf Pondweed. In The

Website of the 10th and 11th Crow Wing Lakes Association. Retrieved April 14, 2013, from crowwing11.org

• Koshere, F. (Photographer). Potamogeton crispus curly pondweed growing in Bone Lake, Wisconsin. [Image of photograph]. Retrieved April 14, 2013, from http://bioweb.uwlax.edu

• Peterson, M. (2003, May). Mature turions (seeds) that washed to shore. In Curly Leaf Identification. Retrieved April 14, 2013, from http://www.lakeorono.org/CurlyleafID.pdf

• Wolf, T. E., & Madsen, J. D. (2003). Seasonal biomass and carbohydrate allocation patterns in Southern Minnesota curlyleaf pondweed populations. J. Aquat. Plant Manage, 41, 113-118.

• (2013). Europe; CABI. Retrieved April 14, 2013, from http://www.plantwise.org/

Acknowledgements

• Zachrie Gutknecht • Christopher Ruhland, PhD• Undergraduate Research Center-Minnesota

State University, Mankato