BioOne sees sustainable scholarly publishing as an inherently collaborative enterprise connecting authors, nonprofit publishers, academic institutions,research libraries, and research funders in the common goal of maximizing access to critical research.

Two New Species of Oribatid Mites of Oripodoidea (Acari: Oribatida) fromBruneiAuthor(s): Sergey G. Ermilov Tapas Chatterjee and David J. MarshallSource: Annales Zoologici, 63(3):393-400. 2013.Published By: Museum and Institute of Zoology, Polish Academy of SciencesDOI: http://dx.doi.org/10.3161/000345413X672456URL: http://www.bioone.org/doi/full/10.3161/000345413X672456

BioOne (www.bioone.org) is a nonprofit, online aggregation of core research in the biological, ecological,and environmental sciences. BioOne provides a sustainable online platform for over 170 journals and bookspublished by nonprofit societies, associations, museums, institutions, and presses.

Your use of this PDF, the BioOne Web site, and all posted and associated content indicates your acceptance ofBioOne’s Terms of Use, available at www.bioone.org/page/terms_of_use.

Usage of BioOne content is strictly limited to personal, educational, and non-commercial use. Commercialinquiries or rights and permissions requests should be directed to the individual publisher as copyright holder.

INTRODUCTION

The oribatid mite fauna of Brunei is poorly known(Aoki 1967, Mahunka 1995, 1997, 2001, 2005). In thecourse of taxonomic identification of oribatid mitesfrom Brunei we found representatives of two newspecies of the superfamily Oripodoidea, one belongingto the genus Maculobates Hammer, 1962 (Haploze-tidae), and one to Perscheloribates Hammer, 1973(Scheloribatidae). The purpose of this paper is to de-scribe and illustrate these two new oripodoid speciesunder the names Maculobates bruneiensis sp. nov.and Perscheloribates interlamellaris sp. nov.

Maculobates is a genus that was proposed by Ham-mer (1962) with Maculobates longiporosus Hammer,1962 as the type species. Currently, the genus compris-es 14 species, which are distributed in the Australian,Antarctic and South Neotropical regions (Subías 2004,

online version 2012). Hence, the genus Maculobates isrecorded for the first time for the Oriental region andBrunei. The main generic characters of the genus arepresented by Hammer (1962) and summarized by Ham-mer (1972) and Balogh and Balogh (1992). An identifi-cation key to species of Maculobates has been pre-sented earlier (Balogh and Balogh 2002).

Perscheloribates is a genus that was proposed byHammer (1973) with Perscheloribates clavatus Ham-mer, 1973 as the type species. Currently, the genuscomprises more than 40 species, distributed in thePantropical and Subtropical regions (Subías 2004,online version 2012, Ermilov and Kalúz 2012). Hence,the genus Maculobates is recorded for the first timefor the Oriental region and Brunei. The main genericcharacters of the genus are presented by Hammer(1973) and summarized by Corpuz-Raros (1980), Baloghand Balogh (1992). The identification keys to selective

TWO NEW SPECIES OF ORIBATID MITES OFORIPODOIDEA (ACARI: ORIBATIDA) FROM BRUNEI

A N N A L E S Z O O L O G I C I (Warszawa), 2013, 63(3): 393-400

SERGEY G. ERMILOV1, *, TAPAS CHATTERJEE2

and DAVID J. MARSHALL3

1Tyumen State University, Semakova 10, Tyumen 625003, Russia; e-mail: ermilovacari@yandex.ru

2Department of Biology, Indian School of Learning, I.S.M. Annexe, P.O.-I.S.M.,Dhanbad-826004, Jharkhand, India; e-mail: drtchatterjee@yahoo.co.in

3Department of Biology, University Brunei Darussalam, Jalan Tungku Link,Gadong BE 1410, Brunei; e-mail: david.marshall@ubd.edu.bn

*Corresponding author

Abstract.— Two new species of the superfamily Oripodoidea, Maculobates bruneiensissp. nov. and Perscheloribates interlamellaris sp. nov., are described from Brunei. Thefirst new species differs from other species of Maculobates by the presence of an anteriornotogastral margin and a specific furrow on ventral side. The second new species differsfrom other species of Perscheloribates by the microfoveolate body surface. The generaMaculobates and Perscheloribates are for the first time recorded in Brunei; Maculobatesis for the first time recorded in the Oriental region.

�

Key words.— oribatid mites, Oripodoidea, Maculobates, Perscheloribates, new species,new record, Brunei.

PL ISSN 0003-4541 © Fundacja Natura optima duxdoi: 10.3161/000345413X672456

species of Perscheloribates have been presented ear-lier (Corpuz-Raros 1980, Balogh and Balogh 2002,Ermilov et al. 2011).

MATERIALS AND METHODS

Material examined. Holotype (female) and fourparatypes (three males and one female) of Maculo-bates bruneiensis sp. nov.; holotype (female) and fiveparatypes (four males and one female) of Perschelori-bates interlamellaris sp. nov.: Brunei, 04°59’N,115°04’E, Pulau Bedukang; Brunei Bay, Brunei Darus-salam, associated with mangrove tree bark andtrunks, collected in May 2012 by David J. Marshall.

Specimens were mounted in lactic acid on tempo-rary cavity slides for measurement and illustration. Allbody measurements are presented in micrometers.Body length was measured in lateral view, from the tipof the rostrum to the posterior edge of the ventral plate,to avoid discrepancies caused by different degrees ofnotogastral distortion. Notogastral width refers to themaximum width in dorsal aspect. Lengths of bodysetae were measured in lateral aspect.

Formulae for leg setation are given in parenthesesaccording to the sequence trochanter–femur–genu–tib-ia–tarsus (famulus included). Formulae for leg soleni-dia are given in square brackets according to thesequence genu–tibia–tarsus.

The general morphological terminology used in thedescriptions follows that presented by Norton andBehan-Pelletier (2009).

TAXONOMY

Maculobates bruneiensis sp. nov.(Figs 1–15)

Etymology. The specific name “bruneiensis”refers to the country origin, Brunei Darussalam.

Diagnosis. Body size 398–448 × 249–282. Rostrumrounded, with two small lateral teeth. Rostral, lamellarand interlamellar setae long, setiform, barbed. Sensillishort, clavate. Anterior notogastral margin distinct.Notogastral setae setiform, smooth; posterior setae h1,h2, h3 and p1 with short flagellate ends, longest, andsetae p2 and p3 shortest. Ventral plate with specific fur-row. Epimeral setae 4a longer than others. Ano-genitalsetae of medium size or short, smooth. Leg setae v’ ongenua I and II, pl’ and pl’’ on tarsi I, l’ on femora III, it’and ft’ on tarsi III, l’ on genua IV absent.

Description. Measurements. Body length 448(holotype), 398–431 (mean 415; four paratypes); bodywidth 265 (holotype), 249–282 (mean 265; fourparatypes).

Integument (Figs 1–4). Body color brown. Bodysurface indistinctly punctate (visible under high magni-fication, × 1000). Prodorsal surface beetwen insertionsof lamellar and interlamellar setae with several longi-tudinal stria. Ventral furrow (f) clearly is bordered themedian part of a ventral plate. Circumgastric band ofsigillae well developed.

Prodorsum (Figs 1, 3, 5). Rostrum weakly protrud-ing, rounded, with two very small lateral teeth (visiblein dorso-anterior view). Lamellae located dorso-later-ally, without cusps. Their length about half of that ofthe prodorsum (see in lateral view). Prolamellar linesdistinct, directed to the rostral teeth. Sublamellar linesand sublamellar porose areas absent. Rostral (ro,57–69), lamellar (le, 65–73) and interlamellar (in,127–143) setae setiform and barbed. Lamellar setaeinserted in medio-distal part of lamellae. Sensilli (ss,32–36) clavate, with long stalk (it covered by the ante-rior margin of notogaster) and well developed, roundeddistally, slightly barbed head. Exobothridial setaeminute (ex, 4), thin, hardly visible.

Notogaster (Figs 1, 4). Anterior notogastral margindistinctly developed, convex and straight. Dorsophrag-mata (D) long. Four pairs of oval porose areas present:Aa 10–12 × 8–10, A1 8–10 × 6–8, A2 8 × 6, A3 6–8 ×4–6. Ten pairs of notogastral setae setiform, smooth;posterior setae h1, h2, h3 and p1 (53–61) with short flag-ellate ends, longer than c, la, lm, lp (36–45) and p2, p3(20–28). Lyrifissures ia not evident; im, ip, ih and ipsand opisthonotal gland openings (gla) located in nor-mal position for Haplozetidae.

Gnathosoma (Figs 6–8). Subcapitulum longer thanwide (98–102 × 73–77). Subcapitular setae setiformand smooth; h (32–41) longer tahn m (12–20) and a(24–28). Two pairs of adoral setae (or1, or2, 8) thick-ened, hook-like distally, barbed. Palps (69–73) withsetation 0–2–1–3–9(+ω). All setae smooth. Solenidionthickened, blunt-ended, attached with eupathidiumacm. Chelicerae (114–118) with two setiform andbarbed setae; cha (36–41) longer than chb (20).Trägårdh’s organ (Tg) distinct.

Epimeral and lateral podosomal regions (Figs2, 3). Epimeral setal formula 3–1–2–2. Setae setiformand slightly barbed; 4a (65–73) longer than 1b (45–53),3b (32–36) and 1a, 1c, 2a, 3a, 4b (16–24). Pedotecta I(Pd I) convex, pedotecta II (Pd II) rounded distally.Custodia (cus) long, blunt-ended. Discidia (dis) weak-ly developed, rounded distally.

Anogenital region (Figs 2, 4). Three pairs of geni-tal (g1–g3, 8–12), one pair of aggenital (ag, 16–20), twopairs of anal (an1, an2, 41–49) and three pairs ofadanal (ad1, ad2, 41–49, ad3, 20–24) setae setiform andsmooth. Lyrifissures iad distinct, located in paranalposition. Circumpedal carinae (cp) distinct.

Legs (Figs 9–15). Claw of all tarsi smooth. Formu-lae of leg setation and solenidia: I (1–5–2–4–17)

394 S. G. ERMILOV, T. CHATTERJEE and D. J. MARSHALL

TWO NEW SPECIES OF ORIBATID MITES OF ORIPODOIDEA FROM BRUNEI 395

Figures 1–2. Maculobates bruneiensis sp. nov., adult: (1) dorsal view (legs not shown); (2) ventral view (palpal setae and legs not shown). Scale bar 100 μm.

[1–2–2], II (1–5–2–4–15) [1–1–2], III (2–2–1–3–13)[1–1–0], IV (1–2–1–3–12) [0–1–0]; homology of setaeand solenidia indicated in Table 1. Famulus short,straight, blunt-ended. Setae v’ on genua I and II, pl’and pl’’ on tarsi I, l’ on femora III, it’ and ft’ on tarsiIII, l’ on genua IV absent. Most setae setiform andbarbed. Solenidia thin, setiform.

Type deposition. The holotype (alcohol) is de-posited in the collection of the Zoological Institute ofthe Russian Academy of Sciences, St. Petersburg, Russia; two paratypes (alcohol) are deposited in thecollection of the Siberian Zoological Museum, Novosi-birsk, Russia; two paratypes (alcohol) are in the per-sonal collection of the first author.

1 2

Leg Trochanter Femur Genu Tibia Tarsus

I v' d, (l), bv'', v'' (l), σ (l), (v), ϕ1, ϕ2 (ft), (tc), (it), (p), (u), (a), s, (pv), v', e, ω1, ω2II v' d, l'1, l'2, bv'', v'' (l), σ (l), (v), ϕ (ft), (tc), (it), (p), (u), (a), s, (pv), ω1, ω2III l', v' d, ev' l', σ l', (v), ϕ ft'', (tc), it'', (p), (u), (a), s, (pv)

IV v' d, ev' d l', (v), ϕ ft'', (tc), (p), (u), (a), s, (pv)

Table 1. Leg setation and solenidia of Maculobates bruneiensis sp. nov.

Roman letters refer to normal setae (e to famulus), Greek letters to solenidia. Single prime (') marks setae on anterior and double prime (") setaeon posterior side of the given leg segment. Parentheses refer to a pseudosymmetrical of setae.

396 S. G. ERMILOV, T. CHATTERJEE and D. J. MARSHALL

Figures 3–8. Maculobates bruneiensis sp. nov., adult: (3) lateral view of prodorsum (gnathosoma, epimeral borders, epimeral setae and legs not shown); (4) lateral view of posterior part of notogaster; (5) rostrum; (6) subcapitulum, left half; (7) palptarsus; (8) anterior part of chelicera.

Scale bars (3, 4) 50 μm, (5, 7, 8) 10 μm, (6) 20 μm.

3

4

5

6

7

8

TWO NEW SPECIES OF ORIBATID MITES OF ORIPODOIDEA FROM BRUNEI 397

Figures 9–15. Maculobates bruneiensis sp. nov., adult: (9) femora of leg I, right, antiaxial view; (10) tarsus of leg I, right, antiaxial view; (11) femurof leg II, right, paraxial view; (12) femur and genu of leg III, left, antiaxial view; (13) tarsus of leg I, left, antiaxial view; (14) trochanter, femur and

genu of leg IV, left, antiaxial view; (15) tibia and tarsus of leg IV, left, antiaxial view. Scale bar 20 μm.

9

10

11

12

13

14

15

Comparison. The new species clearly differs fromother species of Maculobates by the presence of welldeveloped of anterior notogastral margin (versus indis-tinct in the other representatives of Maculobates) andspecific furrow on ventral side – see Integument sub-section (versus absent in the other representatives ofMaculobates).

Perscheloribates interlamellaris sp. nov.(Figs 16–21)

Etymology. The specific name “interlamellaris”refers to the interlamellar setae.

Diagnosis. Body size 498–564 × 332–381. Bodysurface microfoveolate. Lamellae convergent distally.Rostral, lamellar and interlamellar setae long, setiformand barbed. Sensilli short, clavate, barbed. Notogastralsetae short, setiform, indistinctly barbed. Leg setae v’on genua I and II absent.

Description. Measurements. Body length 531(holotype), 498–564 (mean 534; five paratypes); bodywidth 348 (holotype), 332–381 (mean 358; fiveparatypes).

Integument (Figs 16–18). Body color brown. Bodysurface clearly and densely microfoveolate. Circum-gastric band of sigillae well developed.

Prodorsum (Figs 16, 18). Rostrum weakly protrud-ing (visible in dorso-anterior view), rounded. Lamellaelocated dorso-laterally, without cusps, convergent dis-tally, shorter than half of prodorsum (see in lateralview). Prolamellar lines distinct. Sublamellar lines,oval sublamellar porose areas (Al, 6–12 × 4–8) and lat-eral prodorsal carinae (kf) present. Rostral (73–86),lamellar (98–118) and interlamellar (196–225) setaesetiform and barbed. Distance beetwen insertions ofinterlamellar setae shorter than beetwen insertions ofrostral and interlamellar setae. Sensilli (36–45) cla-vate, with short stalk and well developed, rounded dis-tally, slightly barbed head. Exobothridial setae minute(4), thin, hardly visible.

Notogaster (Figs 16, 18). Anterior notogastral mar-gin straight. Dorsophragmata small, oval. Four pairs ofsacculi present (Sa, S1, S2, S3). Ten pairs of notogas-tral setae short (16–20), setiform and indistinctly barb-ed. Lyrifissures ia, im, ip, ih and ips and opisthonotalgland openings located in normal position for Schelori-batidae.

Gnathosoma. Generally, typical for Perschelori-bates (Ermilov et. al. 2011, Ermilov and Kalúz 2012).Subcapitulum longer than wide (102–106 × 147–151).Subcapitular setae setiform and smooth; h (36–41)longer tahn m (20–24) and a (28–32). Two pairs of ado-ral setae (12–16) thickened, hook-like distally, barbed.

398 S. G. ERMILOV, T. CHATTERJEE and D. J. MARSHALL

Figures 16–17. Perscheloribates interlamellaris sp. nov., adult: (16) dorsal view (legs not shown); (17) ventral view (palpal setae and legs not shown). Scale bar 200 μm.

16 17

TWO NEW SPECIES OF ORIBATID MITES OF ORIPODOIDEA FROM BRUNEI 399

Figures 18–21. Perscheloribates interlamellaris sp. nov., adult: (18) lateral view of prodorsum (gnathosoma, epimeral borders, epimeral setaeand legs not shown); (19) tarsus of leg I, right, antiaxial view; (20) anterior part of tibia of leg I, left, antiaxial view; (21) genu of leg I, right, antiaxial

view. Scale bars (18) 100 μm, (19–21) 20 μm.

Palps (90–94) with setation 0–2–1–3–9(+ω). All setae(except some on tarsus) barbed. Solenidion thickened,blunt-ended, attached with eupathidium acm. Chelicerae(151–155) with two setiform and barbed setae; cha (61–65) longer than chb (24–28). Trägårdh’s organ distinct.

Epimeral and lateral podosomal regions (Figs17, 18). Epimeral setal formula 3–1–3–3. Setae setiformand slightly barbed; 1b and 3b (36–41) longer than others (20–28). Pedotecta I convex, pedotecta II round-ed distally. Discidia rounded distally.

Anogenital region (Fig. 17). Four pairs of genital(g1, 24–32, g2–g4, 20–28), one pair of aggenital (12–16),two pairs of anal (28–32) and three pairs of adanal(20–24) setae setiform and indistinctly barbed. Lyrifis-sures iad distinct, located in paranal position. Circum-pedal carinae distinct.

Legs (Figs 19–21). Generally, typical for Persche-loribates (Ermilov et. al. 2011; Ermilov and Kalúz2012). Claw of all tarsi smooth. Formulae of leg seta-tion and solenidia: I (1–5–2–4–19) [1–2–2], II (1–5–2–4–15) [1–1–2], III (2–3–1–3–13) [1–1–0], IV (1–2–2–3–12) [0–1–0]; homology of setae and solenidia indicat-ed in Table 2. Famulus short, straight, blunt-ended.Setae v’ on genua I and II absent. Most setae setiformand barbed. Solenidia thin, setiform.

Type deposition. The holotype (alcohol) is depo-sited in the collection of the Zoological Institute of theRussian Academy of Sciences, St. Petersburg, Russia;three paratypes (alcohol) are deposited in the collec-tion of the Siberian Zoological Museum, Novosibirsk,Russia; two paratypes (alcohol) are in the personal col-lection of the first author.

20

21

18

19

400 S. G. ERMILOV, T. CHATTERJEE and D. J. MARSHALL

Comparison. The new species clearly differs fromother species of Perscheloribates by the microfoveo-late body surface (other representatives of Persche-loribates lack microfoveolae).

ACKNOWLEDGEMENTS

We gratefully acknowledge Dr. Umukusum Shtan-chaeva and Prof. Dr. Luis Subías (Universidad Com-plutense de Madrid, Madrid, Spain) for consultationsand two anonymous reviewers for the valuable com-ments. This study forms part of an investigation of thebiodiversity of the Brunei Estuarine system funded by a grant from the Universiti Brunei Darussalam(UBD/GSR/S&T/16).

REFERENCES

Aoki, J. 1967. A preliminary revision of the family Otocephei-dae (Acari, Cryptostigmata). II. Subfamily Tetracondyli-nae. Bulletin of the National Museum of Natural Science,Tokyo, 10(3): 297–359.

Balogh, J. and P. Balogh. 1992. The oribatid mites genera ofthe world. Vol. 1. Budapest, Hungarian National Museumpress, 263 pp.

Balogh, J. and P. Balogh. 2002. Identification keys to the ori-batid mites of the Extra-Holarctic regions. Vol. 1. Miskolc,Well-Press Publishing Limited, 453 pp.

Corpuz-Raros, L. 1980. Philippine Oribatei (Acarina) V. Sche-loribates Berlese and related genera (Oribatulidae). Kali-kasan, 9(2–3): 169–245.

Ermilov, S. G. and S. Kalúz. 2012. A new subgenus and threenew species of oribatid mites of the family Scheloribatidae

(Acari: Oribatida) from Ecuador. Annales Zoologici, 62(4):773–787.

Ermilov, S. G., Rybalov, L. B. and K. Franke. 2011. Ethiopianoribatid mites of the family Scheloribatidae (Acari: Oriba-tida). African Invertebrates, 52(2): 311–322.

Hammer, M. 1962. Investigations on the oribatid fauna of theAndes Mountains. III. Chile. Det Kongelige Danske Viden-skabernes Selskab Biologiske Skrifter, 13(2): 1–96.

Hammer, M. 1972. Investigations on the oribatid fauna ofTahiti, and some oribatids found on the Atoll Rangiroa.Det Kongelige Danske Videnskabernes Selskab BiologiskeSkrifter, 19(3): 1–66.

Hammer, M. 1973. Oribatids from Tongatapu and Eua, theTonga Islands, and from Upolu, Western Samoa. Det Kon-gelige Danske Videnskabernes Selskab Biologiske Skrift-er, 20(3): 1–70.

Mahunka, S. 1995. Oribatids from Brunei I (Acari: Oribatida)(New and interesting mites from the Geneva MuseumLXXV). Revue Suisse de Zoologie, 102(4): 913–942.

Mahunka, S. 1997. Oribatids from Brunei II (Acari: Oribatida)(Acarologica Genavensia LXXXII). Revue Suisse de Zool-ogie, 104(3): 661–700.

Mahunka, S. 2001. Oribatids from Brunei III (Acari: Oribatida)(Acarologica Genavensia LCI). Revue Suisse de Zoologie,108(2): 317–349.

Mahunka, S. 2005. Oribatids from Brunei IV (Acari: Oribatida)(Acarologica Genavensia CVI). Revue Suisse de Zoologie,112(2): 421–438.

Norton, R. A. and V. M. Behan-Pelletier. 2009. Oribatida. Chap-ter 15. In: G. W. Krantz & D. E. Walter (eds.). A Manual ofAcarology. Texas University Press, Lubbock, 430–564.

Subías, L. S. 2004. Listado sistemático, sinonímico y biogeo-gráfico de los ácaros oribátidos (Acariformes: Oribatida)del mundo (excepto fósiles). Graellsia, 60 (número extra-ordinario), 3–305. Online version accessed in April 2012,564 pp.; http://www.ucm.es/info/zoo/Artropodos/Catalogo.pdf.

Received: January 21, 2013Accepted: July 25, 2013

Leg Trochanter Femur Genu Tibia Tarsus

I v' d, (l), bv'', v'' (l), σ (l), (v), ϕ1, ϕ2 (ft), (tc), (it), (p), (u), (a), s, (pv), (pl), v', e, ω1, ω2II v' d, l'1, l'2, bv'', v'' (l), σ (l), (v), ϕ (ft), (tc), (it), (p), (u), (a), s, (pv), ω1, ω2III l', v' d, l', ev' l', σ l', (v), ϕ (ft), (tc), (it), (p), (u), (a), s, (pv)

IV v' d, ev' d, l' l', (v), ϕ ft'', (tc), (p), (u), (a), s, (pv)

Table 2. Leg setation and solenidia of Perscheloribates interlamellaris sp. nov.

See Table 1 for explanations.