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2005 – 2006 PRODUCT CATALOG

TrueClone:Authentic Full-Length cDNA Clones

Dear Valued Customer,

The completion of the sequencing of the human genome provides us with an enormous amount of data

related to individual genes and their transcripts. There remains, however, a large informational gap between

a gene’s sequence and its biological function, and an even greater gap between the gene’s function and its

complicated interactions with other genes. Unraveling and understanding gene functions and gene networks

will undoubtedly be a great challenge for some time to come.

In 1997, OriGene formulated a vision that the availability of a physical collection of all human genes will be

required to dissect gene function and genetic interactions. After over eight years of dedication, OriGene scientists

have isolated more than 24,000 distinct human full-length cDNA clones representing over 15,000 loci, all in a

unified pCMV expression ready vector. We have built the world’s largest searchable collection of cDNA clones,

the TrueClone CollectionTM. We are proud of the fact that thousands of innovative researchers worldwide have

been taking advantage of these ready-made clones to accelerate their research.

The value of the TrueClone collection is multifaceted:

1. As a source of individual genes. When an authentic human full-length cDNA clone is readily available,

scientists are alleviated of the tedious work of gene cloning and can better direct their time and resources

to more exciting discoveries downstream.

2. As a collection of gene families. For laboratories with relatively focused interests, the TrueClone collection

can be used as an inventory for scientists to pick-and-assemble their own sub-collections and conduct

functional assays. OriGene has pre-assembled several such sub-collections based on biological pathways

or gene families, including protein kinases, GPCR’s, nuclear hormone receptors, and cytochrome

P-450 genes.

3. As a comprehensive collection. Expression-ready TrueClones provide unprecedented opportunities for

scientists to functionally test thousands of human genes in a uniformed cell based assay.This approach,

which we call FlagArray (Full-Length cDNA Annotation of Gene functions), was recently employed by

scientists at the Genomics Institute of the Novartis Research Foundation. Using 20,000 cDNA clones,

including a large number of OriGene TrueClones, they successfully discovered and characterized several

novel modulators of the Wnt signaling pathway.1

OriGene is dedicated to its mission of isolating every single human full-length cDNA clone that represents a

functional transcript in the human genome. We are determined to make TrueClone the ultimate collection: where

every gene can be found, and that every scientist can trust. We understand this is a formidable task. To adhere to

the highest quality standards when isolating authentic clones is costly and time consuming; yet OriGene is

committed to this effort. This strong commitment, coupled with our diverse scientific expertise, has made the

TrueClone collection not only the most comprehensive available, but also the highest quality source. We welcome

all suggestions from our customers to 1) enhance our gene collection 2) provide more extensive downstream

applications and 3) improve our product support. We are especially interested in developing innovative

technologies that functionally assay the human genome in its entirety. If you have an idea, an invention or a

research program potentially involving large subsets of our collection of human full-length cDNA clones, please

do not hesitate to contact us. The perfect collection of human full-length cDNA clones can only be attained via input

and feedback from scientists like you. We look forward to earning your trust and to being your discovery partner.

From the OriGene Team

1. Identification of the Wnt signaling activator leucine-rich repeat in Flightless interaction protein 2 by a genome-wide functional analysis. Liu, J. et al. PNAS, Feb 2005; 102: 1927 - 1932.

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Sections

I. TrueClone™—Ready-cloned non-PCR human full-length cDNA in CMV vectors..................................................................1

Human full-length genes..................................................................................................2

CloneSet: Gene-family based collection .......................................................................10

• Kinase CloneSet

• GPCR CloneSet

• Nuclear Hormone Receptor CloneSet

• Ion Channel CloneSet

• P450 CloneSet

II. HuSH™ ..........................................................................................................45Premade shRNA expression panels ..............................................................................46

pRS vectors......................................................................................................................61

III. Full-length cDNA cloning.............................................................................63Rapid-Screen™ arrayed cDNA libraries ........................................................................64

• Human cDNA Libraries

• Mouse cDNA Libraries

• Rat cDNA Libraries

Rapid-Screen™ Longest-CloneSM services ....................................................................72

• Human 12-Tissue Library Screen

• Mouse 6-Tissue Library Screen

Sure-RACE™ 5’-RACE Kit ...............................................................................................75

• Human 12-Tissue cDNA

• Mouse 12-Tissue cDNA

Yeast two-hybrid expression cDNA libraries ................................................................81

• DupLEX-A™ yeast two-hybrid system kit

• Human Libraries

• Mouse Libraries

• Rat Libraries

• Breast Cancer Libraries

• Prostate Cancer Libraries

• Other Libraries

iiiph: 1.888.267.4436 fax: 1.301.340.9254

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IV. Gene expression ...........................................................................................85Rapid-Scan™: PCR-based gene expression profiling panel........................................86

• Human Tissue Panel (24 Tissues)

• Human Brain Panel (12 Parts)

• Human Breast Cancer (24 Normals/Tumors)

• Mouse Tissue Panel (24 Tissues)

• Mouse Brain Panel (48 Parts/Stages)

• Drosophila Tissue Panel (12 Parts/Stages)

Real-Time-Scan™: Real-Time PCR-based gene expression profiling panel ..............86

• Human Tissue Panel: (48 tissues) NEW

Northern Blot...................................................................................................................95

• Human 12-Tissue RNA Blot I

• Human 12-Tissue RNA Blot II

• Human 6-tissue RNA Blot III

• Mouse 12-Tissue RNA Blot

• Rat 12-Tissue RNA Blot

• Rat 12 Brain-Part RNA Blot

Multiple-Choice™ cDNAs .............................................................................................101

• Human Tissue cDNA

• Mouse Tissue cDNA

• Rat Tissue cDNA

Blue-Ribbon™ Poly A+ and Total RNAs........................................................................105

• Human Tissue RNA

• Mouse Tissue RNA

• Rat Tissue RNA

V. Miscellaneous molecular tools..................................................................109Pre-validated antibodies ...............................................................................................110

• Kinase antibodies

• GPCR antibodies

Protein collections ........................................................................................................130

Quanti-Ladder .............................................................................................................. 140

Broad range DNA size marker (200bp-10kb) with quantity reference

Millennium™ marker ....................................................................................................141

Broad range RNA size marker (0.5kb-9kb)

Rapid-Load.....................................................................................................................142

Innovative PCR compatible loading buffer

Ordering information .......................................................................................143

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TrueClone™Ready-cloned non-PCR human full-length cDNA in CMV vectors

Over 24,000 individual clones

CloneSet™: Gene-family based collection

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Ready-cloned non-PCR human full-length

cDNA in CMV vectors

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All TrueClones are fully searchable using the search

engine on our website. Each clone is represented by

a unique catalog number of 6 digits with a prefix of

“TC”.

OriGene’s TrueClone Collection contains over 24,000

full-length cDNA clones whose nucleotide sequences

can be found in the public domain. They represent

about 85 percent of the transcript sequences that

have been annotated by NCBI.

OriGene’s mission is to clone every single human

full-length transcript. Our cloning effort is aimed at

expanding TrueClone coverage until our mission is

accomplished. Please visit our website often for the

most updated status. If your gene of interest is not

yet included in our collection, you are encouraged to

fill out a cloning request form and our scientists will

make every effort to clone your gene for you.

ADVANTAGES

The most comprehension collection: over 24,000 (and growing)

cDNA clones available

Authentic clones: no PCR artifacts

Expression-Ready: All clones areexpression-optimized in a CMV vector

Quick delivery: Most clones will be shipped within 48 hrs

Product Listing

OriGene’s 24,000 human full-length cDNA clones have individual catalog numbers beginning with TC100001

and growing. Each clone is priced to reflect the respective cloning effort needed to produce it. All of

OriGene’s clones are priced to provide value to our customers in the scientific community.

Visit our website at www.origene.com for price and specifications for each clone.

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Introduction:

Functional genomics, the development and

application of experimental approaches to assess

gene function, begins with a full-length copy of the

gene of interest. Historically, gene cloning was done

one gene at a time through painstaking efforts from

individual laboratories. Organizations such as NCBI,

DDBJ, and EMBL have been centralizing and

archiving gene sequence information, yet they

cannot provide the physical clones needed to allow

researchers to pursue the functional dissection of

these genes, many of which still code for

hypothetical proteins having no known functions.

OriGene Technologies is focused on its mission to

clone every single human gene transcript in the

human transcriptome. We believe that a complete set

of human cDNAs provides the key to unlocking the

fundamentals of human development, physiology

and disease. Since 1997, OriGene Technologies has

been diligent in isolating full-length human genes

from primary cDNA libraries. Utilizing high-

throughput cloning and proprietary Rapid-Screen™

technologies, OriGene has built its TrueCloneTM

Collection — the world’s largest searchable set of

over 24,000 non-redundant full-length human cDNA

clones suitable for protein expression in mammalian

cells or in vitro through coupled translation assays.

All TrueClones are matched to annotated mRNA

reference sequences found in the public domain.

Beginning in 2001, OriGene was one of the first com-

panies to provide ready-cloned genes to the research

community. Our dependable service and dedicated

support have relieved many scientists of the tedious

work of traditional gene cloning. Today, purchasing a

clone has become the standard way of obtaining a

gene; it greatly shortens the gene-cloning timetable

and allows scientists to concentrate their efforts and

resources on exciting downstream applications.

Most importantly, the availability of a large number

of expression-ready human full-length cDNAs makes

it possible to perform global screening of the entire

human transcriptome. Such a systematic approach

has been pioneered by several groups and has

shown to be extremely effective1-8. In one recent

success story, Novartis scientists utilized OriGene’s

TrueClone Collection to identify novel gene

regulators of the WNT signaling pathway1. OriGene is

releasing several such gene array tools to provide

this enabling technology to researchers. The

FlagArrayTM (Full-Length cDNA Annotation of Gene

function array) is one such tool.

3ph: 1.888.267.4436 fax: 1.301.340.9254

1. Identification of the Wnt signaling activator leucine-rich repeat in Flightless interactionprotein 2 by a genome-wide functional analysis Proc Natl Acad Sci U S A. 2005 Feb 8;102(6):1927-32. (Use of over 10,000 OriGeneTrueClones)

2. High throughput functional genomics: identification of novel genes with tumorsuppressor phenotypes. Int J Cancer. 2005 Jan 20;113(3):434-9. (Use of over 17,000 clones)

3. High-throughput Functional Genomics Identifies Genes That Ameliorate Toxicity Due toOxidative Stress in Neuronal HT-22 Cells: GFPT2 Protects Cells Against PeroxideMol Cell Proteomics. 2004 Aug;3(8):834-40. (Use of 5,000 clones, including OriGene'sTrueClones)

4. Identification of p53 regulators by genome-wide functional analysisProc Natl Acad Sci U S A. 2004 Mar 9;101(10):3456-61. (Use of 20,000 clones)

5. Genome-scale functional profiling of the mammalian AP-1 signaling pathway.Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12153-8. (Use of 20,000 clones)

6. Identification of a family of cAMP response element-binding protein coactivators bygenome-scale functional analysis in mammalian cells.Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12147-52. (Use of 20,000 clones)

7. An integrated functional genomics screening program reveals a role for BMP-9 inglucose homeostasis.Nat Biotechnol. 2003 Mar;21(3):294-301. (Use of 8,000 clones encoding secretedproteins)

8. TIP, a T-cell factor identified using high-throughput screening increases survival in agraft-versus-host disease model. Nat Biotechnol. 2003 Mar;21(3):302-7. (Use of 8,000 ESTs encoding putative secretedproteins)

Product Description:

What is a TrueClone?

TrueClone is the world’s largest collection of full-

length human cDNA clones, and is available only

from OriGene. The TrueClone Collection has the

following key features:

1. Comprehensive collection: The TrueClone col-

lection currently contains over 24,000 non-

redundant human cDNAs, each corresponding to

a unique transcript. It is by far the largest source

of human cDNA clones. OriGene has the

commitment and the technical strength to clone

every single human gene.

2. Authentic clones: More than 99% of the clones are

directly isolated from primary cDNA libraries with

no PCR-introduced artifacts. A very small number of

clones are generated by PCR and these are all fully

sequenced to match to their references. These PCR

clones are noted as such on the OriGene website.

3. Expression ready: All genes are directionally

cloned into an expression vector under control of

the CMV promoter, ready to be transfected into

mammalian cells for protein expression. The

uniform vector in all clones facilitates high-

throughput screening approaches.

4. Easy search: OriGene provides several search

engines on our website for convenient identification

of your gene of interest. Please refer to page 7 to see

more search information.

5. Quick delivery: Most TrueClones will be delivered

to you as dried, plasmid DNA within 48 hrs in 2-D

bar-coded tubes.

How is a full-length cDNA defined in OriGene’s TrueClone collection?

Full-length is usually defined as containing the full

open reading frame (ORF) of a gene, but OriGene

TrueClones contain 5’- and 3’-UTRs as well. Currently

OriGene’s clones are defined as full-length when

they satisfy the following three specifications.

1. 5’-junction sequencing: The sequence aligns

upstream of the initiation ATG codon.

2. 3’-junction sequencing: The sequence aligns

downstream of the stop codon.

3. Size verification: Each insert is removed from the

vector by restriction digestion and the size is esti-

mated via gel electrophoresis. This size is matched

against the predicted size of the full-length clone.

OriGene makes all 5’ sequence data available

through its website to facilitate customer searches. In

addition, OriGene has sequenced the ORF of more

than 2,000 TrueClones, including most of the protein

kinases, and has made this information available on

our website. The number of ORF sequenced clones

will increase steadily in the coming years.

How is a non-PCR clone different from aPCR-based clone? Can PCR be used inTrueClone’s downstream applications?

PCR clones may contain mutations that cause

downstream experimental deviations. Non-PCR clones

reflect the true nature of mRNA gene transcripts.

It is a well-documented fact that the PCR process

introduces mutations due to the limited fidelity of Taq

or other PCR enzymes. When a gene is obtained by

the PCR process from a cDNA pool, especially when

the transcript is rare, the high replication cycle

causes the accumulation of mutations. However, this

mutation rate can pale in comparison to the

introduction of mutations due to primer synthesis

errors that can affect 14% of all such PCR clones9.

Even when the final transcripts are sequenced, there

is no way to differentiate whether a mutation is due

to PCR or is simply a naturally occurring SNP. In

addition, very long genes and GC-rich genes can be

impossible to PCR amplify.

One key feature of TrueClones is that they are

authentic clones, isolated directly from cDNA

libraries constructed by a high-fidelity reverse

transcriptase. When using TrueClones, there is no

need to worry about PCR-introduced artifacts.

PCR can be utilized in TrueClone applications.

Starting with an authentic cDNA clone and knowing

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9. Human ORFeome version 1.1: a platform for reverse proteomics. Genome Res. 2004 Oct;14(10B):2128-35.

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its sequence, molecular biologists can safely apply

PCR to amplify a segment of the clone, either the ORF

or a functional domain, for other applications. Using

larger starting amounts of the template DNA, PCR

can be performed with a very low cycle number,

reducing the chance of mutation. More importantly,

by having the sequence information of the template

DNA, it is easy to verify the sequence of the final

product and then to distinguish any PCR-introduced

mutations from naturally occurring SNPs.

How many genes are there in the humangenome? Which commercial source offersthe most complete set of humantranscript clones?

The most recent human genome build (#35) boasts a

large scale improvement over all previous builds and

is likely to become the working genome for quite

some time, providing a more stable gene reference

set. While the total number of gene loci is currently

believed to be 21,787 (2,188 predicted), the expected

number of transcripts including splice variants is

expected to be 1.54 times that, or 33,552.

The largest commercialized collection of cDNA

clones is OriGene’s TrueClone Collection: ~24,000

transcripts covering over 15,000 loci. This is more

than twice as many as those represented in the

mammalian gene collection. Our comprehensive

collection not only provides individual gene users

the best source to find their gene, but also provides

the finest system biology tool available to assess

drug target cross-reactivity, drug action and antigen

recognition. Providing the complete human

transcriptome as non-PCR clones is and remains

OriGene’s mission.

Fig. 1 illustrates the relative relationship between the

predicted human genome and current coverage

provided by OriGene’s TrueClone collection and MGC.

OriGene’sTrueClone collection not only contains more

human full-length cDNA clones than any other

collection, but it also contains more of the difficult and

long clones. Since most cDNA collections all overlap

Fig. 1

Comparison of the predicted number of human transcripts and the

coverage of OriGene TrueClone and MGC. OriGene has more full

length cDNA clones representing a greater number of transcripts

than any other source.

*Number of Transcripts including: Genes, Predicted Genes, Pseudo-

genes and Splice Variants

26,200 (RefSeq)

24,000 OriGene

>33,000 transcripts (21,787 loci)*Human Genome Build 35

12,242 (MGC)

5ph: 1.888.267.4436 fax: 1.301.340.9254

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in their coverage of highly abundant and small cDNA

clones (ORF size less than 3kb), we looked at 200

difficult to isolate clones in this druggable genome

that have an ORF greater than 4 kb.

The result of this analysis is as follows: OriGene has

about 120 of these 200 cDNAs. One of the larger

commercial reagent suppliers has about 27 of these

and the MGC collection has 17 cDNAs representing

these 200 genes. (Fig. 2)

What vectors are employed by TrueClones,and how do they compare to otherexpression vectors?

All of the cDNAs within the TrueClone collection are

cloned into a series of pCMV6 vectors, allowing for

uniform expression screening methods. The vector

plasmid was originally obtained from Dr. David

Russell’s laboratory10,11. The full-length cDNA frag-

ments are directionally inserted downstream from a

eukaryotic transcriptional promoter capable of

driving heterologous gene expression in a variety of

mammalian cell lines and transgenic mice.

Vector Diagram

All three OriGene pCMV6 vectors were constructed

with the same features and multiple cloning site

(MCS), with the exception that pCMV-XL6 has an SP6

transcriptional promoter instead of T7. The basic

backbone of the vector series is presented in the

diagram to the left. (Fig. 3)

Extensive work has been done to engineer the vector

to achieve a high level of transgene expression.

When compared with the popular expression

plasmid, pCDNA3.1 (Invitrogen), pCMV-based

plasmids provide comparable if not higher levels of

transgene expression. (Fig. 4)

Key Functional Features of pCMV6 vectors:

• Vector size: 4.7kb

• Selection marker in E. coli: Ampicillin-resistance

• Selection marker in mammalian cells: None;

requires co-transfection for selection; perfect for

transient transfections

• Promoter for in vivo expression in mammalian

cells: CMV promoter

10. Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme. J Biol Chem. 1989 May 15; 264(14):8222-9. 11. Expression cloning and regulation of steroid 5 alpha-reductase, an enzyme essentialfor male sexual differentiation. J Biol Chem. 1989 Sep 25; 264(27):16249-55.

Comparison of transgene expression levels in pCMV6- and

pCDNA3.1-based plasmids. CAT gene was cloned downstream of

the promoters in the pCMV6 and pCDNA3.1 vectors. In three

independent experiments, the same quantity of plasmid DNA was

transfected into Hela cells and the CAT activity was scored.

T7 Promoter

Sac INot IEcoR I#Bgl IIKpn IEcoRVHind IIISal I#*Xba INot ISma I

# Cloning sites*Lost upon cloning

f1 ori

Amp resistancegene

ColE1 SV40 ori

PolyA signal

cmv promoter

pCMV6-XL44707 bp

Fig. 3

Fig. 4

MCS

Fig. 2

Druggable Genome extracted from information provided by Hopkins,

AL, and Groom, CR, The Druggable Genome, Nat Rev Drug Discov.

2002 9:727-30. In our evaluation, the Druggable Genome consists of

over 3800 unique genes, of which 200 are larger than 4 kb in length.

The comparison above is based upon those large genes.

Fig. 5

Sample applications for individual TrueClone.

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• Promoter for in vitro cell free system:T7 (for pCMV6-

XL4 and pCMV6-XL5) and SP6 for (pCMV6-XL6)

• Cloning sites: EcoRI and SalI; while EcoRI is

preserved, SalI is destroyed upon cloning

• Restriction sites for removing insert: NotI. Two NotI

sites are flanking the vector’s cloning sites

• Cell lines suitable for transfection: COS, HEK293,

Hela, CHO, NIH3T3, Mouse L cell, etc.

• Transcription termination and poly-adenylation

signals: From human growth hormone (hGH) gene

The complete sequence of pCMV6-XL4 has been de-

posited in Genbank with accession number AF067196.

All vector sequences are available at www.origene.com.

What are the utilities of the TrueClone?

TrueClones can be utilized as:

• Individual clones

• Bundled clones

• A complete collection

Individual TrueClones enable a wide spectrum of

gene function studies, as shown in Fig. 5.

Bundles of TrueClone can be selected to perform

focused screening for ligand binding, protein

interaction, or pathway dissection. The bundle can be

constructed based on gene families, such as protein

kinases, GPCRs, nuclear hormone receptors, etc, or it

can be based on functional or cellular localization

similarities, such as secreted molecules, membrane

proteins, etc. OriGene has grouped some key gene

families into CloneSets™ to facilitate such needs.

The complete TrueClone collection can be used to

perform global screening. The comprehensive nature

of the TrueClone and its uniform expression vector

platform make it an ideal tool for a system biology

approach. Exciting new discoveries12, 13 have been

recently made using the TrueClone collection.

How do I search for a gene?

OriGene’s website www.origene.com provides a

wide choice of search criteria.

12. Identification of the Wnt signaling activator leucine-rich repeat in Flightlessinteraction protein 2 by a genome-wide functional analysis. Proc Natl Acad Sci U S A. 2005 Feb 8;102(6):1927-32. 13. High-throughput Functional Genomics Identifies Genes That Ameliorate Toxicity Dueto Oxidative Stress in Neuronal HT-22 Cells: GFPT2 Protects Cells Against Peroxide Mol Cell Proteomics. 2004 Aug;3 (8):834-40.

Producing siRNA by in vitro transcriptionand dicer digestion

Subclone into viral vector for

virus production (eg. Adenovirus, Lentivirus, etc)

proteinexpression in cell-free

system (eg. TNT)

Transient in vivo protein

expression

Subcloning intoother expression

vectors for taggedexpression

TrueClone

Knock-inconstruct fortransgenicexperiment

Transfection ofmammalian cells

Antibody validation

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• BLAST by Nucleotide Sequence, or BLAST by

Protein Sequence

The most sensitive search method is via

BLAST. It generates the most definitive match

to your gene of interest.

• Accession or Catalog Number

The most specific method is by NCBI

accession number. OriGene generally uses the

RefSeq reference number beginning with NM

or XM whenever it is available.

• Protein Domain

An effective method to extract lists of genes

that share a common protein domain.

• Keywords

Keywords are the most flexible but can also

fail to give the desired result. False negative

results are common and it is recommended

that a BLAST search be performed after a

negative keyword search.

• Gene Family

You can view OriGene’s pre-bundled Clone-

Sets™ by gene family from this selection.

If you have difficulty searching or finding what you

are looking for, please call our Customer Care Team

at 888.267.4436 or write to Search Help at OriGene

([email protected]).

Will OriGene provide a custom cloning service whenever a search result is negative?

OriGene is dedicated to clone every human

transcript and we can incorporate your needs in this

effort. When your search result is negative, you are

encouraged to fill out a “Customer Cloning Request

Form” so that our cloning team can put your clone

into our cloning queue. Given the large number of

such requests, we prioritize those customers who

make a commitment to purchase once the clone is

available. You can make such a commitment by

submitting a purchase order. To obtain a quotation,

please contact Customer Care at 1-888-267-4436.

Why are TrueClones priced differently?

Not all clones are created equally. Gene transcripts

differ greatly in many aspects, such as message

abundance, transcript length, GC content, and tissue

distribution. The respective cloning efforts needed to

produce them differ accordingly. OriGene’s

differential pricing reflects the relative difficulty in

obtaining these clones, while still providing value to

all clone users.

Some of the clones represent short and very abundant

transcripts, such as the beta-actin transcript. Such

clones can be obtained with relative ease by high-

throughput sequencing of a small number of clones in

the libraries. Most of the clones in MGC represent such

transcripts. The majority of the TrueClone collection

categorized within this group are priced at $195.

Large and rare clones are difficult to clone and

require individualized effort. Equipped with the

proprietary Rapid-ScreenTM technology, OriGene has

been able to obtain more elusive clones than any

other cloning team, and thus can provide a much

more comprehensive collection. We are continuing

to apply new technologies to clone more genes and

to expand our collection. We believe our offering

provides an excellent value to our customers.

What is provided when I order a gene?

1. OriGene provides purified plasmid DNA (200ng)

dried onto the bottom of a siliconized poly-

propylene tube. The tube has a 2-D bar code on the

bottom which ensures inventory integrity and

accurate shipping, and allows for potential

downstream integration into the end-users LIMS.

2. A copy of our TrueClone Application Guide with

information regarding DNA recovery and down-

stream applications.

3. A certificate of analysis stating your clone’s

specifications.

4. A vial of vector primer (vp1.5) for 5’ sequence

confirmation.

Will OriGene guarantee that a TrueClone is identical to a published reference?

No. There are three factors that contribute to

differences:

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1. Single Nucleotide Polymorphisms (SNP’s). No two

collections will contain exactly the same gene

sequences, and this is true when comparing the

NCBI database references with OriGene’s True-

Clones. Given that polymorphisms exist in 0.1% of

the euchromatic genome, we can expect there to

be a 1 bp difference in each 1000 bp of a reference

on average. Obtained directly from human primary

cDNA libraries, TrueClones are the closest copies to

actual human mRNA transcripts that technology

currently allows, and therefore any such difference

in a TrueClone is likely to be a single nucleotide

polymorphism (SNP).

2. Reference sequences are not always correct. Some

references contain PCR-cloning errors and/or

sequencing errors. In fact, less than one-half of the

curated NCBI RefSeq database is currently placed

into the “reviewed, validated” category.

3. Alternative splicing. Most of OriGene’s clones are

not yet fully sequenced. We qualify our clones by

5’- and 3‘-end sequence matching and insert size

verification. It is possible that some TrueClones

contain a different splice pattern than the

reference.

OriGene guarantees that TrueClones are authentic

cDNA transcripts that encode full-length proteins. If

any of our clones are shown to contain a mutation/

insertion/deletion that disrupts the protein’s open

reading frame, every effort will be made to supply an

appropriate replacement clone from our very large

archive of full-length candidates.

For protein purification, do OriGeneTrueClones provide a fused tag to the expressed protein?

Currently TrueClones are “raw” clones from libraries

supplied without any fused tag. Customers are

encouraged to perform the vector construction in

their own labs. To satisfy such needs for tagged

protein expression, OriGene has teamed up with

Promega to develop a new line of TrueClone

derivatives—the FlexClone series. Flex clones will

enable convenient shuffling of an ORF into a variety

of vectors, facilitating multiple applications,

including tagged protein expression.

Which cloning sites were used to create TrueClones? Which restriction sites can be used to remove the insertfrom a TrueClone?

The cDNA prepared for each library construction was

engineered to have an EcoR1 site on the 5’ end and

an Xho1 site on the 3’ end. The fragments were

ligated into the pCMV vectors pre-cut with EcoR1 and

Sal1. The compatible ends of Xho1 and Sal1 created

a new site that cannot be cut with either.

To liberate the insert, use NotI. NotI sites were

engineered on both sides of the cloning sites.

Please refer to page 6 for more vector information.

What additional information is providedon OriGene’s website when I search for a clone?

OriGene is striving to be your one-stop gene cloning

resource. When you search for a gene on our site, we

provide:

• Direct links to public databases for more detailed

information about each gene

• Sequence specifications for the OriGene clone

• A 24-tissue expression profile: Relative

expression levels of this gene in 24-normal

tissues (data obtained by RT-PCR using OriGene’s

Rapid-Scan expression panels)

• All available products related to this gene,

including protein, antibody and pre-made shRNA

constructs

• The gene family, pathway and function summary

for your gene

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What is a CloneSet™? How manyCloneSets™ does OriGene have? What are the utilities of a CloneSet™?

CloneSets™ are pre-bundled clones based on gene

families. Currently there are several such Clone-

Sets™ within OriGene’s TrueClone collection:

• Protein kinase

• GPCR (G-protein coupled receptors)

• NHR (Nuclear Hormone receptors)

• P450

• Ion Channel

OriGene has created, by far, the most com-

prehensive gene family collections available in the

marketplace, and is proud to offer these important

CloneSets™ to the research community. Such

categorization assists researchers with focused

scientific interest. CloneSets™ are available as a

single product item for focused functional screening.

OriGene CloneSets™ in development include:

• Phosphatases

• Protease

• Lipid kinases

• Phosphodiesterases

• Membrane proteins

• Secreted molecules

To purchase a complete CloneSet, please contact us

at 1.888.267.4436 for terms and quotation.

CloneSet™: Gene-family

based collection

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Kinase CloneSetCat. No. Accession No. Description

TC107659 AB000409 Homo sapiens mRNA for MNK1, complete cds

TC107660 AB023216 Homo sapiens mRNA for KIAA0999 protein, partial cds



TC107661 AF308302 Homo sapiens serologically defined breast cancer antigen NY-BR-96 mRNA, complete cds

TC105333 AK074305 Homo sapiens cDNA FLJ23725 fis, clone HEP14024

TC104797 AK092352 Homo sapiens cDNA FLJ35033 fis, clone OCBBF2016590, weakly similar to CELL SURFACE ANTIGEN

114/A10 PRECURSOR

TC124162 AY792511 Homo sapiens leucine-rich repeat kinase 2 (LRRK2) mRNA, complete cds

TC124163 AY792511 Homo sapiens leucine-rich repeat kinase 2 (LRRK2) mRNA, OriGene unique variant 1

TC124164 AY792511 Homo sapiens leucine-rich repeat kinase 2 (LRRK2) mRNA, OriGene unique variant 2

TC123778 BC002695 Homo sapiens, Similar to KIAA1048 protein, clone MGC:3535 IMAGE:3607681, complete cds

TC107754 BC002695 Homo sapiens, Similar to KIAA1048 protein, clone MGC:3535 IMAGE:3607681, complete cds

TC107753 BC038807 Homo sapiens, Similar to amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 7,

clone MGC:46456 IMAGE:5200755, complete cds

TC107656 BC039243 Homo sapiens, Similar to fibroblast growth factor receptor 2 (bacteria-expressed kinase, keratinocyte

growth factor receptor, craniofacial dysostosis 1, Crouzon syndrome, Pfeiffer syndrome, Jackson-Weiss

syndrome), clone MGC:32962 IMAGE:5277896, com

TC107755 BC041668 Homo sapiens, Similar to receptor-interacting serine-threonine kinase 3, clone MGC:47689 IMAGE:

5767146, complete cds

TC107662 BC052239 Homo sapiens cDNA clone IMAGE:5092935, partial cds

TC116664 NM_000020 Homo sapiens activin A receptor type II-like 1 (ACVRL1)

TC115442 NM_000061 Homo sapiens Bruton agammaglobulinemia tyrosine kinase (BTK)

TC112998 NM_000075 Homo sapiens cyclin-dependent kinase 4 (CDK4)

TC113109 NM_000075 Homo sapiens cyclin-dependent kinase 4 (CDK4), OriGene unique variant 1

TC111932 NM_000141 Homo sapiens fibroblast growth factor receptor 2 (bacteria-expressed kinase, keratinocyte growth

factor receptor, craniofacial dysostosis 1, Crouzon syndrome, Pfeiffer syndrome, Jackson-Weiss

syndrome) (FGFR2), transcript variant 1

TC109231 NM_000142 Homo sapiens fibroblast growth factor receptor 3 (achondroplasia, thanatophoric dwarfism) (FGFR3),

transcript variant 1

TC109267 NM_000162 Homo sapiens glucokinase (hexokinase 4, maturity onset diabetes of the young 2) (GCK), transcript

variant 1

TC120059 NM_000215 Homo sapiens Janus kinase 3 (a protein tyrosine kinase, leukocyte) (JAK3)

TC120061 NM_000222 Homo sapiens v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT)

TC124046 NM_000245 Homo sapiens met proto-oncogene (hepatocyte growth factor receptor) (MET)

TC119991 NM_000294 Homo sapiens phosphorylase kinase, gamma 2 (testis) (PHKG2)

TC119992 NM_000294 Homo sapiens phosphorylase kinase, gamma 2 (testis) (PHKG2), OriGene unique variant 1

TC119871 NM_000455 Homo sapiens serine/threonine kinase 11 (Peutz-Jeghers syndrome) (STK11)

TC119843 NM_000459 Homo sapiens TEK tyrosine kinase, endothelial (venous malformations, multiple cutaneous

and mucosal) (TEK)

TC109227 NM_000604 Homo sapiens fibroblast growth factor receptor 1 (fms-related tyrosine kinase 2, Pfeiffer syndrome)

(FGFR1), OriGene unique variant 1

TC124152 NM_000875 Homo sapiens insulin-like growth factor 1 receptor (IGF1R)

TC119602 NM_000906 Homo sapiens natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A)

(NPR1)

TC107274 NM_001079 Homo sapiens zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), transcript variant 1

TC119434 NM_001105 Homo sapiens activin A receptor, type I (ACVR1)

TC119435 NM_001106 Homo sapiens activin A receptor, type IIB (ACVR2B)

TC119393 NM_001203 Homo sapiens bone morphogenetic protein receptor, type IB (BMPR1B)

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Cat. No. Accession No. Description

TC108987 NM_001204 Homo sapiens bone morphogenetic protein receptor, type II (serine/threonine kinase) (BMPR2),


TC119395 NM_001211 Homo sapiens BUB1 budding uninhibited by benzimidazoles 1 homolog beta (yeast) (BUB1B)

TC108757 NM_001221 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II delta (CAMK2D),




TC119344 NM_001261 Homo sapiens cyclin-dependent kinase 9 (CDC2-related kinase) (CDK9)

TC119354 NM_001274 Homo sapiens CHK1 checkpoint homolog (S. pombe) (CHEK1), OriGene unique variant 1

TC119355 NM_001274 Homo sapiens CHK1 checkpoint homolog (S. pombe) (CHEK1)

TC119358 NM_001278 Homo sapiens conserved helix-loop-helix ubiquitous kinase (CHUK)

TC124169 NM_001315 Homo sapiens mitogen-activated protein kinase 14 (MAPK14), transcript variant 1

TC119302 NM_001319 Homo sapiens casein kinase 1, gamma 2 (CSNK1G2)

TC119321 NM_001348 Homo sapiens death-associated protein kinase 3 (DAPK3)

TC119139 NM_001569 Homo sapiens interleukin-1 receptor-associated kinase 1 (IRAK1)


TC119156 NM_001616 Homo sapiens activin A receptor, type II (ACVR2)

TC119158 NM_001619 Homo sapiens adrenergic, beta, receptor kinase 1 (ADRBK1)

TC119161 NM_001626 Homo sapiens v-akt murine thymoma viral oncogene homolog 2 (AKT2)

TC119127 NM_001654 Homo sapiens v-raf murine sarcoma 3611 viral oncogene homolog (ARAF)

TC119055 NM_001715 Homo sapiens B lymphoid tyrosine kinase (BLK), OriGene unique variant 1

TC119056 NM_001715 Homo sapiens B lymphoid tyrosine kinase (BLK)

TC119060 NM_001721 Homo sapiens BMX non-receptor tyrosine kinase (BMX)

TC110839 NM_001726 Homo sapiens bromodomain, testis-specific (BRDT), transcript variant 2

TC119077 NM_001744 Homo sapiens calcium/calmodulin-dependent protein kinase IV (CAMK4)

TC111605 NM_001786 Homo sapiens cell division cycle 2, G1 to S and G2 to M (CDC2), transcript variant 1

TC119016 NM_001787 Homo sapiens cell division cycle 2-like 1 (PITSLRE proteins) (CDC2L1), transcript variant 1


TC109060 NM_001798 Homo sapiens cyclin-dependent kinase 2 (CDK2), transcript variant 1

TC119024 NM_001799 Homo sapiens cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase) (CDK7)

TC118967 NM_001892 Homo sapiens casein kinase 1, alpha 1 (CSNK1A1)

TC118968 NM_001893 Homo sapiens casein kinase 1, delta (CSNK1D), OriGene unique variant 1

TC109127 NM_001895 Homo sapiens casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 2

TC118969 NM_001896 Homo sapiens casein kinase 2, alpha prime polypeptide (CSNK2A2)

TC123974 NM_001954 Homo sapiens discoidin domain receptor family, member 1 (DDR1), transcript variant 2

TC118918 NM_001982 Homo sapiens v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) (ERBB3), transcript

variant 1

TC118883 NM_002005 Homo sapiens feline sarcoma oncogene (FES)

TC111632 NM_002011 Homo sapiens fibroblast growth factor receptor 4 (FGFR4), transcript variant 1

TC118894 NM_002020 Homo sapiens fms-related tyrosine kinase 4 (FLT4), transcript variant 2

TC118895 NM_002020 Homo sapiens fms-related tyrosine kinase 4 (FLT4), OriGene unique variant 1

TC118862 NM_002037 Homo sapiens FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 1

TC108487 NM_002082 Homo sapiens G protein-coupled receptor kinase 6 (GRK6), transcript variant 2

TC118824 NM_002093 Homo sapiens glycogen synthase kinase 3 beta (GSK3B), OriGene unique variant 1

TC118825 NM_002093 Homo sapiens glycogen synthase kinase 3 beta (GSK3B), OriGene unique variant 2

TC118826 NM_002093 Homo sapiens glycogen synthase kinase 3 beta (GSK3B)

TC118838 NM_002110 Homo sapiens hemopoietic cell kinase (HCK)

TC118761 NM_002227 Homo sapiens Janus kinase 1 (a protein tyrosine kinase) (JAK1)

TC118716 NM_002253 Homo sapiens kinase insert domain receptor (a type III receptor tyrosine kinase) (KDR)

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TC118711 NM_002314 Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1

TC123883 NM_002314 Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1

TC108490 NM_002350 Homo sapiens v-yes-1 Yamaguchi sarcoma viral related oncogene homolog (LYN), OriGene unique

variant 1

TC108492 NM_002350 Homo sapiens v-yes-1 Yamaguchi sarcoma viral related oncogene homolog (LYN)

TC118702 NM_002376 Homo sapiens MAP/microtubule affinity-regulating kinase 3 (MARK3), OriGene unique variant 1

TC118703 NM_002376 Homo sapiens MAP/microtubule affinity-regulating kinase 3 (MARK3)

TC118669 NM_002401 Homo sapiens mitogen-activated protein kinase kinase kinase 3 (MAP3K3), transcript variant 2

TC110949 NM_002419 Homo sapiens mitogen-activated protein kinase kinase kinase 11 (MAP3K11)


TC108482 NM_002497 Homo sapiens NIMA (never in mitosis gene a)-related kinase 2 (NEK2)

TC109479 NM_002498 Homo sapiens NIMA (never in mitosis gene a)-related kinase 3 (NEK3), OriGene unique variant 1

TC109481 NM_002498 Homo sapiens NIMA (never in mitosis gene a)-related kinase 3 (NEK3), transcript variant 1

TC118578 NM_002530 Homo sapiens neurotrophic tyrosine kinase, receptor, type 3 (NTRK3)

TC118579 NM_002530 Homo sapiens neurotrophic tyrosine kinase, receptor, type 3 (NTRK3), OriGene unique variant 1

TC107950 NM_002576 Homo sapiens p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) (PAK1)

TC118562 NM_002577 Homo sapiens p21 (CDKN1A)-activated kinase 2 (PAK2)

TC118563 NM_002578 Homo sapiens p21 (CDKN1A)-activated kinase 3 (PAK3)

TC118532 NM_002595 Homo sapiens PCTAIRE protein kinase 2 (PCTK2)

TC118538 NM_002610 Homo sapiens pyruvate dehydrogenase kinase, isoenzyme 1 (PDK1), nuclear gene encoding

mitochondrial protein, OriGene unique variant 1

TC118539 NM_002610 Homo sapiens pyruvate dehydrogenase kinase, isoenzyme 1 (PDK1), nuclear gene encoding

mitochondrial protein

TC118540 NM_002611 Homo sapiens pyruvate dehydrogenase kinase, isoenzyme 2 (PDK2)


TC118543 NM_002613 Homo sapiens 3-phosphoinositide dependent protein kinase-1 (PDPK1), OriGene unique variant 1

TC118544 NM_002613 Homo sapiens 3-phosphoinositide dependent protein kinase-1 (PDPK1)

TC110975 NM_002648 Homo sapiens pim-1 oncogene (PIM1)

TC118448 NM_002730 Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1

TC108498 NM_002731 Homo sapiens protein kinase, cAMP-dependent, catalytic, beta (PRKACB), OriGene unique variant 1

TC108499 NM_002731 Homo sapiens protein kinase, cAMP-dependent, catalytic, beta (PRKACB), transcript variant 2

TC118449 NM_002732 Homo sapiens protein kinase, cAMP-dependent, catalytic, gamma (PRKACG)

TC124082 NM_002737 Homo sapiens protein kinase C, alpha (PRKCA)

TC118454 NM_002738 Homo sapiens protein kinase C, beta 1 (PRKCB1), transcript variant 2

TC112717 NM_002739 Homo sapiens protein kinase C, gamma (PRKCG)

TC118455 NM_002740 Homo sapiens protein kinase C, iota (PRKCI)

TC118456 NM_002741 Homo sapiens protein kinase N1 (PKN1), transcript variant 2

TC118457 NM_002742 Homo sapiens protein kinase D1 (PRKD1)

TC118459 NM_002744 Homo sapiens protein kinase C, zeta (PRKCZ), OriGene unique variant 1

TC118460 NM_002744 Homo sapiens protein kinase C, zeta (PRKCZ), OriGene unique variant 2

TC118461 NM_002744 Homo sapiens protein kinase C, zeta (PRKCZ)

TC118463 NM_002747 Homo sapiens mitogen-activated protein kinase 4 (MAPK4)







TC118424 NM_002755 Homo sapiens mitogen-activated protein kinase kinase 1 (MAP2K1)

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TC109629 NM_002757 Homo sapiens mitogen-activated protein kinase kinase 5 (MAP2K5), OriGene unique variant 1

TC124173 NM_002757 Homo sapiens mitogen-activated protein kinase kinase 5 (MAP2K5), transcript variant B

TC118432 NM_002758 Homo sapiens mitogen-activated protein kinase kinase 6 (MAP2K6), transcript variant 1

TC118433 NM_002759 Homo sapiens eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2)

TC118434 NM_002760 Homo sapiens protein kinase, Y-linked (PRKY)

TC118391 NM_002821 Homo sapiens PTK7 protein tyrosine kinase 7 (PTK7), transcript variant PTK7-1

TC109662 NM_002822 Homo sapiens PTK9 protein tyrosine kinase 9 (PTK9), transcript variant 1

TC109663 NM_002822 Homo sapiens PTK9 protein tyrosine kinase 9 (PTK9), OriGene unique variant 1

TC118322 NM_002880 Homo sapiens v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1), OriGene unique variant 1

TC118323 NM_002880 Homo sapiens v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1)

TC118278 NM_002929 Homo sapiens G protein-coupled receptor kinase 1 (GRK1)

TC118295 NM_002953 Homo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1

TC112721 NM_002958 Homo sapiens RYK receptor-like tyrosine kinase (RYK), transcript variant 2


TC118306 NM_002969 Homo sapiens mitogen-activated protein kinase 12 (MAPK12), OriGene unique variant 1






TC118183 NM_003137 Homo sapiens SFRS protein kinase 1 (SRPK1)


TC108474 NM_003159 Homo sapiens cyclin-dependent kinase-like 5 (CDKL5), OriGene unique variant 1

TC118139 NM_003160 Homo sapiens aurora kinase C (AURKC)

TC118140 NM_003161 Homo sapiens ribosomal protein S6 kinase, 70kDa, polypeptide 1 (RPS6KB1)

TC118141 NM_003161 Homo sapiens ribosomal protein S6 kinase, 70kDa, polypeptide 1 (RPS6KB1), OriGene unique variant 1

TC118115 NM_003177 Homo sapiens spleen tyrosine kinase (SYK)

TC118119 NM_003188 Homo sapiens mitogen-activated protein kinase kinase kinase 7 (MAP3K7), transcript variant A

TC118147 NM_003215 Homo sapiens tec protein tyrosine kinase (TEC)

TC118072 NM_003242 Homo sapiens transforming growth factor, beta receptor II (70/80kDa) (TGFBR2)

TC118032 NM_003318 Homo sapiens TTK protein kinase (TTK)

TC118033 NM_003318 Homo sapiens TTK protein kinase (TTK), OriGene unique variant 1

TC118038 NM_003328 Homo sapiens TXK tyrosine kinase (TXK)

TC111048 NM_003331 Homo sapiens tyrosine kinase 2 (TYK2)

TC117994 NM_003384 Homo sapiens vaccinia related kinase 1 (VRK1)

TC117999 NM_003390 Homo sapiens WEE1 homolog (S. pombe) (WEE1)

TC117908 NM_003503 Homo sapiens CDC7 cell division cycle 7 (S. cerevisiae) (CDC7)

TC117907 NM_003576 Homo sapiens serine/threonine kinase 24 (STE20 homolog, yeast) (STK24)

TC117867 NM_003582 Homo sapiens dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (DYRK3), transcript

variant 1


variant 1

TC117877 NM_003600 Homo sapiens serine/threonine kinase 6 (STK6), transcript variant 2

TC117892 NM_003618 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), OriGene unique

variant 1

TC117893 NM_003618 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3)

TC117843 NM_003656 Homo sapiens calcium/calmodulin-dependent protein kinase I (CAMK1)

TC117844 NM_003656 Homo sapiens calcium/calmodulin-dependent protein kinase I (CAMK1), OriGene unique variant 1

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TC109943 NM_003668 Homo sapiens mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript

variant 1

TC109944 NM_003668 Homo sapiens mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), OriGene

unique variant 1

TC109951 NM_003674 Homo sapiens cyclin-dependent kinase (CDC2-like) 10 (CDK10), OriGene unique variant 1

TC109952 NM_003674 Homo sapiens cyclin-dependent kinase (CDC2-like) 10 (CDK10), transcript variant 1

TC117862 NM_003684 Homo sapiens MAP kinase interacting serine/threonine kinase 1 (MKNK1)

TC117810 NM_003688 Homo sapiens calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK)


TC109966 NM_003718 Homo sapiens cell division cycle 2-like 5 (cholinesterase-related cell division controller) (CDC2L5),


TC108466 NM_003804 Homo sapiens receptor (TNFRSF)-interacting serine-threonine kinase 1 (RIPK1)

TC117734 NM_003821 Homo sapiens receptor-interacting serine-threonine kinase 2 (RIPK2)

TC117735 NM_003821 Homo sapiens receptor-interacting serine-threonine kinase 2 (RIPK2), OriGene unique variant 1

TC109987 NM_003831 Homo sapiens RIO kinase 3 (yeast) (RIOK3), transcript variant 1

TC106951 NM_003845 Homo sapiens dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4), OriGene

unique variant 1

TC106952 NM_003845 Homo sapiens dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4)

TC117716 NM_003852 Homo sapiens transcriptional intermediary factor 1 (TIF1), transcript variant 2

TC107962 NM_003913 Homo sapiens PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B), transcript variant 1


TC111095 NM_003948 Homo sapiens cyclin-dependent kinase-like 2 (CDC2-related kinase) (CDKL2)

TC117656 NM_003952 Homo sapiens ribosomal protein S6 kinase, 70kDa, polypeptide 2 (RPS6KB2), OriGene unique variant 1

TC124062 NM_003952 Homo sapiens ribosomal protein S6 kinase, 70kDa, polypeptide 2 (RPS6KB2), transcript variant 1


TC101068 NM_003957 Homo sapiens BR serine/threonine kinase 2 (BRSK2)

TC117643 NM_003985 Homo sapiens tyrosine kinase, non-receptor, 1 (TNK1)

TC109075 NM_003992 Homo sapiens CDC-like kinase 3 (CLK3), transcript variant phclk3

TC109076 NM_003992 Homo sapiens CDC-like kinase 3 (CLK3), OriGene unique variant 1hclk3

TC117645 NM_003993 Homo sapiens CDC-like kinase 2 (CLK2), transcript variant 1

TC117646 NM_003995 Homo sapiens natriuretic peptide receptor B/guanylate cyclase B (atrionatriuretic peptide receptor B) (NPR2)

TC117573 NM_004071 Homo sapiens CDC-like kinase 1 (CLK1), OriGene unique variant 1

TC117574 NM_004071 Homo sapiens CDC-like kinase 1 (CLK1)

TC117577 NM_004073 Homo sapiens polo-like kinase 3 (Drosophila) (PLK3)

TC109209 NM_004103 Homo sapiens PTK2B protein tyrosine kinase 2 beta (PTK2B), transcript variant 2

TC124086 NM_004119 Homo sapiens fms-related tyrosine kinase 3 (FLT3)


TC109999 NM_004197 Homo sapiens serine/threonine kinase 19 (STK19), OriGene unique variant 1

TC117519 NM_004203 Homo sapiens membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase

(PKMYT1), transcript variant 1

TC117533 NM_004226 Homo sapiens serine/threonine kinase 17b (apoptosis-inducing) (STK17B)

TC108895 NM_004302 Homo sapiens activin A receptor, type IB (ACVR1B), transcript variant 1

TC117431 NM_004327 Homo sapiens breakpoint cluster region (BCR), transcript variant 1

TC117433 NM_004329 Homo sapiens bone morphogenetic protein receptor, type IA (BMPR1A)

TC117437 NM_004333 Homo sapiens v-raf murine sarcoma viral oncogene homolog B1 (BRAF)

TC110841 NM_004336 Homo sapiens BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1)

TC117389 NM_004383 Homo sapiens c-src tyrosine kinase (CSK)

TC108512 NM_004384 Homo sapiens casein kinase 1, gamma 3 (CSNK1G3)

TC117404 NM_004409 Homo sapiens dystrophia myotonica-protein kinase (DMPK)

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TC117346 NM_004431 Homo sapiens EPH receptor A2 (EPHA2)



TC111626 NM_004439 Homo sapiens EPH receptor A5 (EPHA5), transcript variant 1


TC117355 NM_004441 Homo sapiens EPH receptor B1 (EPHB1)




TC125233 NM_004448 Homo sapiens v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived

oncogene homolog (avian) (ERBB2), transcript variant 1

TC117313 NM_004517 Homo sapiens integrin-linked kinase (ILK)

TC117279 NM_004560 Homo sapiens receptor tyrosine kinase-like orphan receptor 2 (ROR2)


TC111005 NM_004586 Homo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 3 (RPS6KA3), OriGene unique variant 1

TC111006 NM_004586 Homo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 3 (RPS6KA3)

TC108520 NM_004612 Homo sapiens transforming growth factor, beta receptor I (activin A receptor type II-like kinase, 53kDa)

(TGFBR1)

TC117238 NM_004635 Homo sapiens mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3)


TC117200 NM_004690 Homo sapiens LATS, large tumor suppressor, homolog 1 (Drosophila) (LATS1)”

TC117223 NM_004714 Homo sapiens dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B (DYRK1B), transcript

variant a


TC108525 NM_004734 Homo sapiens doublecortin and CaM kinase-like 1 (DCAMKL1)

TC117159 NM_004759 Homo sapiens mitogen-activated protein kinase-activated protein kinase 2 (MAPKAPK2), transcript

variant 1

TC117160 NM_004760 Homo sapiens serine/threonine kinase 17a (apoptosis-inducing) (STK17A)

TC117141 NM_004783 Homo sapiens TAO kinase 2 (TAOK2)


TC117098 NM_004850 Homo sapiens Rho-associated, coiled-coil containing protein kinase 2 (ROCK2)



TC110899 NM_004958 Homo sapiens FK506 binding protein 12-rapamycin associated protein 1 (FRAP1)

TC117052 NM_004963 Homo sapiens guanylate cyclase 2C (heat stable enterotoxin receptor) (GUCY2C)

TC117059 NM_004972 Homo sapiens Janus kinase 2 (a protein tyrosine kinase) (JAK2)

TC117005 NM_005012 Homo sapiens receptor tyrosine kinase-like orphan receptor 1 (ROR1)


TC116959 NM_005044 Homo sapiens protein kinase, X-linked (PRKX)

TC116928 NM_005104 Homo sapiens bromodomain containing 2 (BRD2)

TC111128 NM_005109 Homo sapiens oxidative-stress responsive 1 (OXSR1)

TC116914 NM_005157 Homo sapiens v-abl Abelson murine leukemia viral oncogene homolog 1 (ABL1), transcript variant a

TC116916 NM_005160 Homo sapiens adrenergic, beta, receptor kinase 2 (ADRBK2)

TC116917 NM_005160 Homo sapiens adrenergic, beta, receptor kinase 2 (ADRBK2), OriGene unique variant 1

TC116883 NM_005163 Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1)


TC116882 NM_005211 Homo sapiens colony stimulating factor 1 receptor, formerly McDonough feline sarcoma viral (v-fms)

oncogene homolog (CSF1R)

TC110878 NM_005228 Homo sapiens epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene

homolog, avian) (EGFR), transcript variant 1

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TC109197 NM_005233 Homo sapiens EPH receptor A3 (EPHA3), transcript variant 1

TC116863 NM_005235 Homo sapiens v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) (ERBB4), OriGene

unique variant 1

TC116869 NM_005246 Homo sapiens fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) (FER)

TC116870 NM_005248 Homo sapiens Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog (FGR)

TC116876 NM_005255 Homo sapiens cyclin G associated kinase (GAK)

TC116815 NM_005307 Homo sapiens G protein-coupled receptor kinase 4 (GRK4), OriGene unique variant 1

TC110910 NM_005308 Homo sapiens G protein-coupled receptor kinase 5 (GRK5)

TC116769 NM_005356 Homo sapiens lymphocyte-specific protein tyrosine kinase (LCK), OriGene unique variant 1

TC116770 NM_005356 Homo sapiens lymphocyte-specific protein tyrosine kinase (LCK)


TC116792 NM_005400 Homo sapiens protein kinase C, epsilon (PRKCE)

TC116752 NM_005417 Homo sapiens v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC), transcript

variant 1

TC116728 NM_005424 Homo sapiens tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1)

TC116734 NM_005433 Homo sapiens v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1 (YES1)

TC110105 NM_005465 Homo sapiens v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) (AKT3),


TC116679 NM_005546 Homo sapiens IL2-inducible T-cell kinase (ITK)

TC116659 NM_005569 Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 2a

TC116680 NM_005592 Homo sapiens muscle, skeletal, receptor tyrosine kinase (MUSK)


TC116597 NM_005627 Homo sapiens serum/glucocorticoid regulated kinase (SGK)

TC116599 NM_005627 Homo sapiens serum/glucocorticoid regulated kinase (SGK), OriGene unique variant 1

TC124179 NM_005734 Homo sapiens homeodomain interacting protein kinase 3 (HIPK3)

TC116515 NM_005762 Homo sapiens tripartite motif-containing 28 (TRIM28)

TC116528 NM_005781 Homo sapiens tyrosine kinase, non-receptor, 2 (TNK2), transcript variant 1


TC116464 NM_005881 Homo sapiens branched chain ketoacid dehydrogenase kinase (BCKDK)

TC116439 NM_005906 Homo sapiens male germ cell-associated kinase (MAK)


TC116404 NM_005975 Homo sapiens PTK6 protein tyrosine kinase 6 (PTK6)

TC124087 NM_005975 Homo sapiens PTK6 protein tyrosine kinase 6 (PTK6)

TC116411 NM_005990 Homo sapiens serine/threonine kinase 10 (STK10)

TC116371 NM_006035 Homo sapiens CDC42 binding protein kinase beta (DMPK-like) (CDC42BPB)

TC116294 NM_006182 Homo sapiens discoidin domain receptor family, member 2 (DDR2)

TC111664 NM_006201 Homo sapiens PCTAIRE protein kinase 1 (PCTK1), transcript variant 1

TC116256 NM_006206 Homo sapiens platelet-derived growth factor receptor, alpha polypeptide (PDGFRA)

TC116261 NM_006213 Homo sapiens phosphorylase kinase, gamma 1 (muscle) (PHKG1)

TC116246 NM_006251 Homo sapiens protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1), transcript variant 1

TC116247 NM_006252 Homo sapiens protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2)

TC116249 NM_006254 Homo sapiens protein kinase C, delta (PRKCD), transcript variant 1

TC116250 NM_006255 Homo sapiens protein kinase C, eta (PRKCH)

TC108568 NM_006256 Homo sapiens protein kinase N2 (PKN2)

TC116251 NM_006257 Homo sapiens protein kinase C, theta (PRKCQ)

TC116252 NM_006258 Homo sapiens protein kinase, cGMP-dependent, type I (PRKG1)

TC116254 NM_006259 Homo sapiens protein kinase, cGMP-dependent, type II (PRKG2)


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TC116192 NM_006285 Homo sapiens testis-specific kinase 1 (TESK1)

TC108283 NM_006293 Homo sapiens TYRO3 protein tyrosine kinase (TYRO3)


TC116203 NM_006296 Homo sapiens vaccinia related kinase 2 (VRK2), OriGene unique variant 1

TC116209 NM_006301 Homo sapiens mitogen-activated protein kinase kinase kinase 12 (MAP3K12), OriGene unique variant 1

TC116132 NM_006343 Homo sapiens c-mer proto-oncogene tyrosine kinase (MERTK)


TC111156 NM_006374 Homo sapiens serine/threonine kinase 25 (STE20 homolog, yeast) (STK25), OriGene unique variant 1


variant 2

TC124175 NM_006549 Homo sapiens calcium/calmodulin-dependent protein kinase kinase 2, beta (CAMKK2), transcript

variant 1

TC116033 NM_006549 Homo sapiens calcium/calmodulin-dependent protein kinase kinase 2, beta (CAMKK2), OriGene unique

variant 1

TC116034 NM_006549 Homo sapiens calcium/calmodulin-dependent protein kinase kinase 2, beta (CAMKK2), OriGene unique

variant 2

TC110237 NM_006575 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1



TC115926 NM_006712 Homo sapiens FAST kinase (FASTK), transcript variant 1

TC101252 NM_006742 Homo sapiens protein serine kinase H1 (PSKH1)

TC115810 NM_006852 Homo sapiens tousled-like kinase 2 (TLK2)

TC115811 NM_006852 Homo sapiens tousled-like kinase 2 (TLK2), OriGene unique variant 1

TC115812 NM_006852 Homo sapiens tousled-like kinase 2 (TLK2), OriGene unique variant 2

TC115825 NM_006875 Homo sapiens pim-2 oncogene (PIM2)

TC110233 NM_007079 Homo sapiens protein tyrosine phosphatase type IVA, member 3 (PTP4A3), transcript variant 2

TC115660 NM_007170 Homo sapiens testis-specific kinase 2 (TESK2)


TC115669 NM_007181 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), OriGene unique

variant 1

TC110240 NM_007194 Homo sapiens CHK2 checkpoint homolog (S. pombe) (CHEK2), transcript variant 1



TC115604 NM_007284 Homo sapiens PTK9L protein tyrosine kinase 9-like (A6-related protein) (PTK9L)

TC115594 NM_007314 Homo sapiens v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene)

(ABL2), transcript variant b

TC115567 NM_007371 Homo sapiens bromodomain containing 3 (BRD3)

TC111954 NM_012119 Homo sapiens cell cycle related kinase (CCRK), OriGene unique variant 1

TC124172 NM_012119 Homo sapiens cell cycle related kinase (CCRK), transcript variant 2

TC108453 NM_012290 Homo sapiens tousled-like kinase 1 (TLK1)

TC115413 NM_012395 Homo sapiens PFTAIRE protein kinase 1 (PFTK1), OriGene unique variant 1

TC115380 NM_012424 Homo sapiens ribosomal protein S6 kinase, 52kDa, polypeptide 1 (RPS6KC1)

TC115335 NM_013233 Homo sapiens serine threonine kinase 39 (STE20/SPS1 homolog, yeast) (STK39)

TC111251 NM_013254 Homo sapiens TANK-binding kinase 1 (TBK1)

TC115312 NM_013257 Homo sapiens serum/glucocorticoid regulated kinase-like (SGKL), transcript variant 1

TC108619 NM_013355 Homo sapiens protein kinase N3 (PKN3)

TC115246 NM_013392 Homo sapiens nuclear receptor binding protein (NRBP)

TC115206 NM_014002 Homo sapiens inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon (IKBKE)

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TC107297 NM_014215 Homo sapiens insulin receptor-related receptor (INSRR)

TC115138 NM_014226 Homo sapiens renal tumor antigen (RAGE), OriGene unique variant 1

TC115139 NM_014226 Homo sapiens renal tumor antigen (RAGE)


TC115101 NM_014299 Homo sapiens bromodomain containing 4 (BRD4), transcript variant short


TC115063 NM_014365 Homo sapiens heat shock 22kDa protein 8 (HSPB8)






TC114987 NM_014496 Homo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 6 (RPS6KA6)

TC120535 NM_014572 Homo sapiens LATS, large tumor suppressor, homolog 2 (Drosophila) (LATS2)

TC100382 NM_014602 Homo sapiens phosphoinositide-3-kinase, regulatory subunit 4, p150 (PIK3R4)

TC114908 NM_014683 Homo sapiens unc-51-like kinase 2 (C. elegans) (ULK2)

TC114874 NM_014720 Homo sapiens STE20-like kinase (yeast) (SLK)

TC114824 NM_014791 Homo sapiens maternal embryonic leucine zipper kinase (MELK)

TC114791 NM_014826 Homo sapiens CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant A

TC114801 NM_014840 Homo sapiens AMP-activated protein kinase family member 5 (ARK5)

TC100957 NM_015000 Homo sapiens serine/threonine kinase 38 like (STK38L)

TC111200 NM_015148 Homo sapiens PAS domain containing serine/threonine kinase (PASK)

TC101100 NM_015690 Homo sapiens serine/threonine kinase 36 (fused homolog, Drosophila) (STK36)

TC114621 NM_015716 Homo sapiens misshapen-like kinase 1 (zebrafish) (MINK1), transcript variant 1


(FGFR1), transcript variant 2

TC114584 NM_015906 Homo sapiens tripartite motif-containing 33 (TRIM33), transcript variant alpha

TC114557 NM_015978 Homo sapiens TNNI3 interacting kinase (TNNI3K)

TC111598 NM_015981 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha (CAMK2A), transcript

variant 1

TC114467 NM_016123 Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), OriGene unique variant 1


TC114352 NM_016231 Homo sapiens nemo like kinase (NLK)

TC114377 NM_016281 Homo sapiens TAO kinase 3 (TAOK3)


TC114264 NM_016440 Homo sapiens vaccinia related kinase 3 (VRK3), OriGene unique variant 1

TC114274 NM_016457 Homo sapiens protein kinase D2 (PRKD2), OriGene unique variant 1


TC110272 NM_016513 Homo sapiens intestinal cell (MAK-like) kinase (ICK), transcript variant 2

TC114204 NM_016542 Homo sapiens Mst3 and SOK1-related kinase (MST4)

TC114182 NM_016653 Homo sapiens sterile alpha motif and leucine zipper containing kinase AZK (ZAK)

TC114149 NM_016733 Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 2b

TC109198 NM_017449 Homo sapiens EPH receptor B2 (EPHB2), transcript variant 1

TC114124 NM_017490 Homo sapiens MAP/microtubule affinity-regulating kinase 2 (MARK2), transcript variant 1

TC114079 NM_017572 Homo sapiens MAP kinase interacting serine/threonine kinase 2 (MKNK2)

TC110519 NM_017593 Homo sapiens BMP2 inducible kinase (BMP2K), transcript variant 2

TC114000 NM_017719 Homo sapiens SNF-1 related kinase (SNRK)

TC100365 NM_017771 Homo sapiens PX domain containing serine/threonine kinase (PXK)

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TC108694 NM_017886 Homo sapiens hypothetical protein FLJ20574 (FLJ20574)

TC113782 NM_017988 Homo sapiens SCY1-like 2 (S. cerevisiae) (SCYL2)

TC113560 NM_018343 Homo sapiens RIO kinase 2 (yeast) (RIOK2)

TC113491 NM_018401 Homo sapiens serine/threonine kinase 32B (STK32B)

TC113492 NM_018401 Homo sapiens serine/threonine kinase 32B (STK32B), OriGene unique variant 1

TC113493 NM_018401 Homo sapiens serine/threonine kinase 32B (STK32B), OriGene unique variant 2

TC113504 NM_018423 Homo sapiens serine/threonine/tyrosine kinase 1 (STYK1), OriGene unique variant 1

TC113505 NM_018423 Homo sapiens serine/threonine/tyrosine kinase 1 (STYK1)

TC113506 NM_018423 Homo sapiens serine/threonine/tyrosine kinase 1 (STYK1), OriGene unique variant 2

TC113461 NM_018492 Homo sapiens T-LAK cell-originated protein kinase (TOPK)

TC113432 NM_018571 Homo sapiens amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2 (ALS2CR2)

TC113408 NM_018650 Homo sapiens MAP/microtubule affinity-regulating kinase 1 (MARK1)

TC112599 NM_019884 Homo sapiens glycogen synthase kinase 3 alpha (GSK3A)

TC113203 NM_020168 Homo sapiens p21(CDKN1A)-activated kinase 6 (PAK6)

TC111363 NM_020247 Homo sapiens chaperone, ABC1 activity of bc1 complex like (S. pombe) (CABC1)

TC110589 NM_020341 Homo sapiens p21(CDKN1A)-activated kinase 7 (PAK7), transcript variant 1

TC108612 NM_020421 Homo sapiens aarF domain containing kinase 1 (ADCK1)

TC113079 NM_020423 Homo sapiens ezrin-binding partner PACE-1 (PACE-1), transcript variant 1

TC108424 NM_020439 Homo sapiens calcium/calmodulin-dependent protein kinase IG (CAMK1G)

TC113095 NM_020547 Homo sapiens anti-Mullerian hormone receptor, type II (AMHR2)

TC113100 NM_020639 Homo sapiens receptor-interacting serine-threonine kinase 4 (RIPK4), OriGene unique variant 1

TC113041 NM_020666 Homo sapiens CDC-like kinase 4 (CLK4), OriGene unique variant 1

TC113042 NM_020666 Homo sapiens CDC-like kinase 4 (CLK4)

TC113047 NM_020680 Homo sapiens SCY1-like 1 (S. cerevisiae) (SCYL1)

TC107055 NM_020922 Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant 1

TC112978 NM_021133 Homo sapiens ribonuclease L (2’,5’-oligoisoadenylate synthetase-dependent) (RNASEL)


TC112991 NM_021158 Homo sapiens tribbles homolog 3 (Drosophila) (TRIB3)


TC112559 NM_021913 Homo sapiens AXL receptor tyrosine kinase (AXL), transcript variant 1

TC112854 NM_022048 Homo sapiens casein kinase 1, gamma 1 (CSNK1G1)”


TC109238 NM_023029 Homo sapiens fibroblast growth factor receptor 2 (bacteria-expressed kinase, keratinocyte growth factor

receptor, craniofacial dysostosis 1, Crouzon syndrome, Pfeiffer syndrome, Jackson-Weiss syndrome)




TC112299 NM_024046 Homo sapiens hypothetical protein MGC8407 (MGC8407)

TC111981 NM_024800 Homo sapiens NIMA (never in mitosis gene a)- related kinase 11 (NEK11)



TC111013 NM_025144 Homo sapiens alpha-kinase 1 (ALPK1), OriGene unique variant 1

TC111024 NM_025144 Homo sapiens alpha-kinase 1 (ALPK1)




TC109313 NM_030952 Homo sapiens likely ortholog of rat SNF1/AMP-activated protein kinase (SNARK)


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TC107258 NM_031417 Homo sapiens MAP/microtubule affinity-regulating kinase 4

(MARK4)

TC107535 NM_031464 Homo sapiens ribosomal protein S6 kinase-like 1 (RPS6KL1)

TC110673 NM_031480 Homo sapiens RIO kinase 1 (yeast) (RIOK1), transcript variant 1

TC111461 NM_031965 Homo sapiens germ cell associated 2 (haspin) (GSG2)

TC107277 NM_032017 Homo sapiens Ser/Thr-like kinase (MGC4796)

TC106635 NM_032028 Homo sapiens serine/threonine kinase 22D (spermiogenesis associated) (STK22D)

TC106670 NM_032037 Homo sapiens serine/threonine protein kinase SSTK (SSTK)



TC108012 NM_032409 Homo sapiens PTEN induced putative kinase 1 (PINK1)


TC103147 NM_032844 Homo sapiens microtubule associated serine/threonine kinase-like (MASTL)


TC109561 NM_033018 Homo sapiens PCTAIRE protein kinase 1 (PCTK1), transcript variant 2

TC101858 NM_033019 Homo sapiens PCTAIRE protein kinase 1 (PCTK1), OriGene unique variant 1

TC124171 NM_033020 Homo sapiens tripartite motif-containing 33 (TRIM33), transcript variant beta


TC101725 NM_033118 Homo sapiens myosin light chain kinase 2, skeletal muscle (MYLK2)

TC101381 NM_033266 Homo sapiens endoplasmic reticulum to nucleus signalling 2 (ERN2)

TC120376 NM_033550 Homo sapiens TP53 regulating kinase (TP53RK)

TC120138 NM_052841 Homo sapiens serine/threonine kinase 22C (spermiogenesis associated) (STK22C)


TC120190 NM_053006 Homo sapiens serine/threonine kinase 22B (spermiogenesis associated) (STK22B)

TC109468 NM_053025 Homo sapiens myosin, light polypeptide kinase (MYLK), transcript variant 1

TC109471 NM_053030 Homo sapiens myosin, light polypeptide kinase (MYLK), transcript variant 5

TC120354 NM_080823 Homo sapiens src-related kinase lacking C-terminal regulatory tyrosine and N-terminal myristylation

sites (SRMS)

TC120556 NM_130436 Homo sapiens dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A (DYRK1A), transcript

variant 2





TC111522 NM_138995 Homo sapiens myosin IIIB (MYO3B)




TC108197 NM_139062 Homo sapiens casein kinase 1, delta (CSNK1D), transcript variant 2

TC120591 NM_139158 Homo sapiens amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 7 (ALS2CR7)

TC100071 NM_139355 Homo sapiens megakaryocyte-associated tyrosine kinase (MATK), transcript variant 1



TC100039 NM_145001 Homo sapiens serine/threonine kinase 32A (STK32A)

TC109628 NM_145109 Homo sapiens mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant B


TC100186 NM_145259 Homo sapiens activin A receptor, type IC (ACVR1C), OriGene unique variant 1

TC120776 NM_145259 Homo sapiens activin A receptor, type IC (ACVR1C), OriGene unique variant 2

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TC110026 NM_145319 Homo sapiens mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant 2

TC100191 NM_145331 Homo sapiens mitogen-activated protein kinase kinase kinase 7 (MAP3K7), transcript variant B

TC100241 NM_145686 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 2

TC100654 NM_152221 Homo sapiens casein kinase 1, epsilon (CSNK1E), transcript variant 1


TC100617 NM_152619 Homo sapiens doublecortin and CaM kinase-like 2 (DCAMKL2)


TC111874 NM_152696 Homo sapiens homeodomain interacting protein kinase 1 (HIPK1), transcript variant 2

TC100783 NM_152835 Homo sapiens PDLIM1 interacting kinase 1 like (PDIK1L)

TC100784 NM_152835 Homo sapiens PDLIM1 interacting kinase 1 like (PDIK1L), OriGene unique variant 1

TC100826 NM_153335 Homo sapiens protein kinase LYK5 (LYK5), transcript variant 3

TC100346 NM_153361 Homo sapiens hypothetical protein MGC42105 (MGC42105)

TC100851 NM_153498 Homo sapiens calcium/calmodulin-dependent protein kinase ID (CAMK1D), transcript variant 2


TC110120 NM_170693 Homo sapiens serum/glucocorticoid regulated kinase 2 (SGK2), transcript variant 1

TC110121 NM_170693 Homo sapiens serum/glucocorticoid regulated kinase 2 (SGK2), OriGene unique variant 1

TC109000 NM_171825 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha (CAMK2A),


TC109001 NM_171825 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha (CAMK2A), OriGene

unique variant 1

TC109004 NM_172078 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II beta (CAMK2B), transcript

variant 2

TC109006 NM_172081 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II beta (CAMK2B), transcript

variant 5

TC109009 NM_172115 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II delta (CAMK2D), transcript

variant 4

TC108250 NM_172127 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II delta (CAMK2D), transcript

variant 1

TC100733 NM_172170 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma (CAMK2G), transcript

variant 3

TC100734 NM_172170 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma (CAMK2G),

OriGene unique variant 1







TC101272 NM_173354 Homo sapiens SNF1-like kinase (SNF1LK)

TC101117 NM_173575 Homo sapiens serine/threonine kinase 32C (STK32C)

TC101143 NM_173677 Homo sapiens hypothetical protein FLJ40852 (FLJ40852), OriGene unique variant 1


TC101352 NM_175866 Homo sapiens U2AF homology motif (UHM) kinase 1 (UHMK1)



TC108395 NM_177560 Homo sapiens casein kinase 2, alpha 1 polypeptide (CSNK2A1), OriGene unique variant 1

TC107077 NM_177990 Homo sapiens p21(CDKN1A)-activated kinase 7 (PAK7), transcript variant 2


TC107239 NM_178564 Homo sapiens nuclear receptor binding protein 2 (NRBP2)

TC107542 NM_182691 Homo sapiens SFRS protein kinase 2 (SRPK2), transcript variant 2

TC107658 U12140 Human tyrosine kinase receptor p145TRK-B (TRK-B) mRNA, complete cds

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TC107115 XM_027237 Homo sapiens mitogen-activated protein kinase kinase kinase 9 (MAP3K9)

TC103895 XM_032034 Homo sapiens syntrophin associated serine/threonine kinase (SAST)

TC120042 XM_032491 Homo sapiens inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta (IKBKB),


TC120053 XM_032491 Homo sapiens inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta (IKBKB),


TC120031 XM_032491 Homo sapiens inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta (IKBKB)

TC104537 XM_038150 PREDICTED: Homo sapiens microtubule associated serine/threonine kinase 3 (MAST3)

TC107086 XM_038576 PREDICTED: Homo sapiens hypothetical protein BC007901 (LOC91461)

TC107120 XM_040819 Homo sapiens similar to myosin light chain kinase (MLCK) (LOC91807)

TC116431 XM_041314 Homo sapiens KIAA0781 protein (KIAA0781)

TC116731 XM_042066 PREDICTED: Homo sapiens mitogen-activated protein kinase kinase kinase 1 (MAP3K1)

TC105253 XM_044630 Homo sapiens DKFZP434C131 protein (DKFZP434C131)

TC113998 XM_047355 PREDICTED: Homo sapiens KIAA1765 protein (KIAA1765)

TC105033 XM_055766 Homo sapiens mitogen-activated protein kinase 3 (MAPK3)

TC107663 XM_065695 Homo sapiens hypothetical gene LOC130382 (LOC130382) mRNA

TC107090 XM_114973 PREDICTED: Homo sapiens EphA6 (EPHA6)

TC107118 XM_208846 Homo sapiens similar to p21-activated protein kinase I (LOC283806)

TC107128 XM_210040 Homo sapiens similar to MAP/ERK kinase kinase 5; apoptosis signal regulating kinase [Homo sapiens]

(LOC286417)

TC107121 XM_211325 Homo sapiens LOC284084 (LOC284084)

TC107122 XM_290778 Homo sapiens apoptosis-associated tyrosine kinase (AATK)

TC107123 XM_290796 Homo sapiens serine/threonine protein kinase TAO1 homolog (KIAA1361)

TC107082 XM_290898 Homo sapiens KIAA0472 protein (KIAA0472)

TC107093 XM_291107 Homo sapiens NIMA (never in mitosis gene a)-related kinase 1 (NEK1)

TC107099 XM_291304 Homo sapiens hypothetical protein MGC43306 (MGC43306)

GPCR CloneSetCat. No. Accession No. Description

TC105790 AF411117 Homo sapiens G protein-coupled receptor (GPR103) mRNA, complete cds

TC106281 BC016940 Homo sapiens, Similar to olfactory receptor, family 2, subfamily A, member 4, clone IMAGE:4424116

TC107900 ENST00000317625 Database:core Gene:ENSG00000181109 Clone:AC109341 Contig:AC109341.7.1.202761 Chr:11

Basepair:5712017 Status:pseudogene

TC116997 NM_000024 Homo sapiens adrenergic, beta-2-, receptor, surface (ADRB2)

TC117219 NM_000025 Homo sapiens adrenergic, beta-3-, receptor (ADRB3)

TC115220 NM_000054 Homo sapiens arginine vasopressin receptor 2 (nephrogenic diabetes insipidus) (AVPR2)

TC111113 NM_000115 Homo sapiens endothelin receptor type B (EDNRB), transcript variant 1

TC109246 NM_000145 Homo sapiens follicle stimulating hormone receptor (FSHR), transcript variant 1

TC120082 NM_000160 Homo sapiens glucagon receptor (GCGR)

TC110906 NM_000164 Homo sapiens gastric inhibitory polypeptide receptor (GIPR)

TC119966 NM_000316 Homo sapiens parathyroid hormone receptor 1 (PTHR1)

TC119936 NM_000369 Homo sapiens thyroid stimulating hormone receptor (TSHR)

TC119946 NM_000388 Homo sapiens calcium-sensing receptor (hypocalciuric hypercalcemia 1, severe neonatal

hyperparathyroidism) (CASR)

TC119911 NM_000406 Homo sapiens gonadotropin-releasing hormone receptor (GNRHR)

TC119816 NM_000524 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 1A (HTR1A)

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TC119819 NM_000529 Homo sapiens melanocortin 2 receptor (adrenocorticotropic hormone) (MC2R)

TC110858 NM_000579 Homo sapiens chemokine (C-C motif) receptor 5 (CCR5)


TC119794 NM_000623 Homo sapiens bradykinin receptor B2 (BDKRB2)

TC119771 NM_000634 Homo sapiens interleukin 8 receptor, alpha (IL8RA)

TC109079 NM_000647 Homo sapiens chemokine (C-C motif) receptor 2 (CCR2), transcript variant A

TC109082 NM_000648 Homo sapiens chemokine (C-C motif) receptor 2 (CCR2), transcript variant B

TC119756 NM_000674 Homo sapiens adenosine A1 receptor (ADORA1)

TC119757 NM_000675 Homo sapiens adenosine A2a receptor (ADORA2A)

TC119758 NM_000676 Homo sapiens adenosine A2b receptor (ADORA2B)

TC119759 NM_000677 Homo sapiens adenosine A3 receptor (ADORA3)

TC119760 NM_000678 Homo sapiens adrenergic, alpha-1D-, receptor (ADRA1D)

TC110821 NM_000679 Homo sapiens adrenergic, alpha-1B-, receptor (ADRA1B)

TC108910 NM_000680 Homo sapiens adrenergic, alpha-1A-, receptor (ADRA1A), transcript variant 1

TC119761 NM_000681 Homo sapiens adrenergic, alpha-2A-, receptor (ADRA2A)



TC124067 NM_000683 Homo sapiens adrenergic, alpha-2C-, receptor (ADRA2C), OriGene unique variant 1

TC124091 NM_000683 Homo sapiens adrenergic, alpha-2C-, receptor (ADRA2C)

TC119765 NM_000684 Homo sapiens adrenergic, beta-1-, receptor (ADRB1), OriGene unique variant 1

TC108918 NM_000685 Homo sapiens angiotensin II receptor, type 1 (AGTR1), transcript variant 1

TC119766 NM_000686 Homo sapiens angiotensin II receptor, type 2 (AGTR2)

TC110836 NM_000706 Homo sapiens arginine vasopressin receptor 1A (AVPR1A)

TC119717 NM_000707 Homo sapiens arginine vasopressin receptor 1B (AVPR1B)



TC119735 NM_000738 Homo sapiens cholinergic receptor, muscarinic 1 (CHRM1)

TC110855 NM_000739 Homo sapiens cholinergic receptor, muscarinic 2 (CHRM2), transcript variant 4



TC119692 NM_000794 Homo sapiens dopamine receptor D1 (DRD1)

TC119693 NM_000795 Homo sapiens dopamine receptor D2 (DRD2), transcript variant 1

TC124065 NM_000797 Homo sapiens dopamine receptor D4 (DRD4), OriGene unique variant 1



TC119635 NM_000823 Homo sapiens growth hormone releasing hormone receptor (GHRHR)

TC119643 NM_000838 Homo sapiens glutamate receptor, metabotropic 1 (GRM1)





TC119649 NM_000844 Homo sapiens glutamate receptor, metabotropic 7 (GRM7), transcript variant 1


TC119662 NM_000861 Homo sapiens histamine receptor H1 (HRH1)

TC119612 NM_000863 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 1B (HTR1B)

TC119613 NM_000864 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 1D (HTR1D)

TC119614 NM_000865 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 1E (HTR1E)

TC108583 NM_000866 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 1F (HTR1F)

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TC119615 NM_000868 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 2C (HTR2C)

TC119616 NM_000870 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 4 (HTR4), transcript variant b

TC119617 NM_000871 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 6 (HTR6)

TC119618 NM_000872 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled) (HTR7),

transcript variant a

TC119604 NM_000909 Homo sapiens neuropeptide Y receptor Y1 (NPY1R)


TC119606 NM_000912 Homo sapiens opioid receptor, kappa 1 (OPRK1)

TC109521 NM_000913 Homo sapiens opiate receptor-like 1 (OPRL1), transcript variant 2

TC119609 NM_000914 Homo sapiens opioid receptor, mu 1 (OPRM1), transcript variant MOR-1

TC119610 NM_000916 Homo sapiens oxytocin receptor (OXTR)

TC119557 NM_000952 Homo sapiens platelet-activating factor receptor (PTAFR)

TC101345 NM_000953 Homo sapiens prostaglandin D2 receptor (DP) (PTGDR)

TC119559 NM_000955 Homo sapiens prostaglandin E receptor 1 (subtype EP1), 42kDa (PTGER1)

TC119560 NM_000956 Homo sapiens prostaglandin E receptor 2 (subtype EP2), 53kDa (PTGER2)

TC111682 NM_000957 Homo sapiens prostaglandin E receptor 3 (subtype EP3) (PTGER3), OriGene unique variant 1

TC119561 NM_000958 Homo sapiens prostaglandin E receptor 4 (subtype EP4) (PTGER4)

TC119562 NM_000959 Homo sapiens prostaglandin F receptor (FP) (PTGFR)

TC119563 NM_000960 Homo sapiens prostaglandin I2 (prostacyclin) receptor (IP) (PTGIR)

TC119481 NM_001049 Homo sapiens somatostatin receptor 1 (SSTR1)





TC119454 NM_001057 Homo sapiens tachykinin receptor 2 (TACR2)

TC119455 NM_001058 Homo sapiens tachykinin receptor 1 (TACR1), transcript variant long

TC111034 NM_001060 Homo sapiens thromboxane A2 receptor (TBXA2R), transcript variant 2

TC119444 NM_001118 Homo sapiens adenylate cyclase activating polypeptide 1 (pituitary) receptor type I (ADCYAP1R1)


TC119288 NM_001296 Homo sapiens chemokine binding protein 2 (CCBP2)

TC119314 NM_001337 Homo sapiens chemokine (C-X3-C motif) receptor 1 (CX3CR1)

TC119262 NM_001400 Homo sapiens endothelial differentiation, sphingolipid G-protein-coupled receptor, 1 (EDG1)

TC119263 NM_001401 Homo sapiens endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 2 (EDG2),


TC119229 NM_001462 Homo sapiens formyl peptide receptor-like 1 (FPRL1), transcript variant 1

TC119233 NM_001466 Homo sapiens frizzled homolog 2 (Drosophila) (FZD2)

TC109253 NM_001470 Homo sapiens gamma-aminobutyric acid (GABA) B receptor, 1 (GABBR1), transcript variant 1

TC119210 NM_001504 Homo sapiens chemokine (C-X-C motif) receptor 3 (CXCR3)

TC119211 NM_001505 Homo sapiens G protein-coupled receptor 30 (GPR30)



TC119185 NM_001525 Homo sapiens hypocretin (orexin) receptor 1 (HCRTR1)

TC119187 NM_001526 Homo sapiens hypocretin (orexin) receptor 2 (HCRTR2)

TC119177 NM_001557 Homo sapiens interleukin 8 receptor, beta (IL8RB)

TC119090 NM_001704 Homo sapiens brain-specific angiogenesis inhibitor 3 (BAI3)

TC119065 NM_001727 Homo sapiens bombesin-like receptor 3 (BRS3)

TC119072 NM_001736 Homo sapiens complement component 5 receptor 1 (C5a ligand) (C5R1)

TC119074 NM_001742 Homo sapiens calcitonin receptor (CALCR)

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TC109039 NM_001784 Homo sapiens CD97 antigen (CD97), OriGene unique variant 1

TC109040 NM_001784 Homo sapiens CD97 antigen (CD97), transcript variant 2

TC118980 NM_001837 Homo sapiens chemokine (C-C motif) receptor 3 (CCR3), transcript variant 1


TC118984 NM_001841 Homo sapiens cannabinoid receptor 2 (macrophage) (CNR2)

TC118901 NM_001957 Homo sapiens endothelin receptor type A (EDNRA)

TC118924 NM_001992 Homo sapiens coagulation factor II (thrombin) receptor (F2R)

TC118857 NM_002029 Homo sapiens formyl peptide receptor 1 (FPR1)

TC118858 NM_002030 Homo sapiens formyl peptide receptor-like 2 (FPRL2)

TC110900 NM_002036 Homo sapiens Duffy blood group (FY)

TC118878 NM_002062 Homo sapiens glucagon-like peptide 1 receptor (GLP1R)


TC118704 NM_002377 Homo sapiens MAS1 oncogene (MAS1)

TC118656 NM_002386 Homo sapiens melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor) (MC1R)

TC118568 NM_002511 Homo sapiens neuromedin B receptor (NMBR)

TC110963 NM_002531 Homo sapiens neurotensin receptor 1 (high affinity) (NTSR1)

TC118599 NM_002563 Homo sapiens purinergic receptor P2Y, G-protein coupled, 1 (P2RY1)

TC118600 NM_002565 Homo sapiens pyrimidinergic receptor P2Y, G-protein coupled, 4 (P2RY4)

TC118351 NM_002921 Homo sapiens retinal G protein coupled receptor (RGR), OriGene unique variant 1

TC118062 NM_003301 Homo sapiens thyrotropin-releasing hormone receptor (TRHR)

TC117992 NM_003382 Homo sapiens vasoactive intestinal peptide receptor 2 (VIPR2)

TC117951 NM_003467 Homo sapiens chemokine (C-X-C motif) receptor 4 (CXCR4), transcript variant 2







TC117889 NM_003614 Homo sapiens galanin receptor 3 (GALR3)

TC117852 NM_003667 Homo sapiens leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5)

TC117757 NM_003775 Homo sapiens endothelial differentiation, G-protein-coupled receptor 6 (EDG6)

TC117689 NM_003876 Homo sapiens chromosome 17 open reading frame 35 (C17orf35)

TC117654 NM_003950 Homo sapiens coagulation factor II (thrombin) receptor-like 3 (F2RL3)

TC117661 NM_003965 Homo sapiens chemokine (C-C motif) receptor-like 2 (CCRL2)

TC117662 NM_003967 Homo sapiens putative neurotransmitter receptor (PNR)

TC117639 NM_003979 Homo sapiens G protein-coupled receptor, family C, group 5, member A (GPCR5A)

TC117601 NM_004054 Homo sapiens complement component 3a receptor 1 (C3AR1)

TC117575 NM_004072 Homo sapiens chemokine-like receptor 1 (CMKLR1)


TC117536 NM_004122 Homo sapiens growth hormone secretagogue receptor (GHSR), transcript variant 1b

TC109533 NM_004154 Homo sapiens pyrimidinergic receptor P2Y, G-protein coupled, 6 (P2RY6), transcript variant 4





TC110860 NM_004382 Homo sapiens corticotropin releasing hormone receptor 1 (CRHR1)

TC117230 NM_004624 Homo sapiens vasoactive intestinal peptide receptor 1 (VIPR1), OriGene unique variant 1

TC124135 NM_004624 Homo sapiens vasoactive intestinal peptide receptor 1 (VIPR1)

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TC117226 NM_004720 Homo sapiens endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 4 (EDG4)

TC117166 NM_004767 Homo sapiens G-protein coupled receptor 37 like 1 (GPR37L1)


TC117047 NM_004951 Homo sapiens Epstein-Barr virus induced gene 2 (lymphocyte-specific G protein-coupled receptor) (EBI2)

TC116961 NM_005048 Homo sapiens parathyroid hormone receptor 2 (PTHR2)

TC116918 NM_005161 Homo sapiens angiotensin II receptor-like 1 (AGTRL1)







TC116839 NM_005283 Homo sapiens chemokine (C motif) receptor 1 (XCR1)

















TC112677 NM_005302 Homo sapiens G protein-coupled receptor 37 (endothelin receptor type B-like) (GPR37)

TC108542 NM_005303 Homo sapiens G protein-coupled receptor 40 (GPR40), OriGene unique variant 1




TC116819 NM_005314 Homo sapiens gastrin-releasing peptide receptor (GRPR)



TC116556 NM_005682 Homo sapiens G protein-coupled receptor 56 (GPR56), transcript variant 1





TC116534 NM_005795 Homo sapiens calcitonin receptor-like (CALCRL)

TC111748 NM_005866 Homo sapiens opioid receptor, sigma 1 (OPRS1), transcript variant 1

TC116445 NM_005912 Homo sapiens melanocortin 4 receptor (MC4R)


TC116399 NM_005958 Homo sapiens melatonin receptor 1A (MTNR1A)

TC116403 NM_005972 Homo sapiens pancreatic polypeptide receptor 1 (PPYR1)

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TC116358 NM_006018 Homo sapiens G protein-coupled receptor 109B (GPR109B)

TC116382 NM_006056 Homo sapiens neuromedin U receptor 1 (NMUR1)

TC116313 NM_006086 Homo sapiens tubulin, beta 3 (TUBB3)



TC116043 NM_006564 Homo sapiens chemokine (C-X-C motif) receptor 6 (CXCR6)

TC115965 NM_006639 Homo sapiens cysteinyl leukotriene receptor 1 (CYSLTR1)



TC115645 NM_007223 Homo sapiens putative G protein coupled receptor (GPR)


TC115624 NM_007264 Homo sapiens adrenomedullin receptor (ADMR)


TC115571 NM_009585 Homo sapiens angiotensin II receptor, type 1 (AGTR1), transcript variant 2


TC115507 NM_012152 Homo sapiens endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 7 (EDG7)


TC115441 NM_012302 Homo sapiens latrophilin 2 (LPHN2)

TC115397 NM_012344 Homo sapiens neurotensin receptor 2 (NTSR2)

TC115400 NM_012360 Homo sapiens olfactory receptor, family 1, subfamily F, member 1 (OR1F1)

TC115401 NM_012368 Homo sapiens olfactory receptor, family 2, subfamily C, member 1 (OR2C1)



TC115222 NM_013447 Homo sapiens egf-like module containing, mucin-like, hormone receptor-like 2 (EMR2), transcript variant 1

TC115039 NM_014322 Homo sapiens opsin 3 (encephalopsin, panopsin) (OPN3)


TC115018 NM_014430 Homo sapiens cell death-inducing DFFA-like effector b (CIDEB)

TC110395 NM_014499 Homo sapiens purinergic receptor P2Y, G-protein coupled, 10 (P2RY10), transcript variant 1



TC108169 NM_015236 Homo sapiens latrophilin 3 (LPHN3)

TC111611 NM_016083 Homo sapiens cannabinoid receptor 1 (brain) (CNR1), transcript variant 1

TC114355 NM_016235 Homo sapiens G protein-coupled receptor, family C, group 5, member B (GPRC5B)



TC111283 NM_016557 Homo sapiens chemokine (C-C motif) receptor-like 1 (CCRL1), transcript variant 2

TC114218 NM_016568 Homo sapiens relaxin 3 receptor 1 (RLN3R1)



TC113833 NM_017986 Homo sapiens G protein-coupled receptor 172B (GPR172B)



TC113412 NM_018654 Homo sapiens G protein-coupled receptor, family C, group 5, member D (GPRC5D)

TC113346 NM_018949 Homo sapiens urotensin 2 receptor (UTS2R)

TC113275 NM_018969 Homo sapiens G-protein coupled receptor 173 (GPR173)



TC113233 NM_019839 Homo sapiens leukotriene B4 receptor 2 (LTB4R2)

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TC110388 NM_019858 Homo sapiens gene rich cluster, A gene (GRCA), OriGene unique variant 1-2

TC109327 NM_019860 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled) (HTR7),

transcript variant b


TC113202 NM_020167 Homo sapiens neuromedin U receptor 2 (NMUR2)


TC113111 NM_020377 Homo sapiens cysteinyl leukotriene receptor 2 (CYSLTR2)



TC113097 NM_020633 Homo sapiens vomeronasal 1 receptor 1 (VN1R1)





TC110534 NM_022036 Homo sapiens G protein-coupled receptor, family C, group 5, member C (GPRC5C), transcript variant 1

TC107967 NM_022049 Homo sapiens G-protein coupled receptor 88 (GPR88)

TC112783 NM_022159 Homo sapiens EGF, latrophilin and seven transmembrane domain containing 1 (ELTD1)






TC108833 NM_024531 Homo sapiens G protein-coupled receptor 172A (GPR172A)


TC109424 NM_030774 Homo sapiens olfactory receptor, family 51, subfamily E, member 2 (OR51E2)


TC107947 NM_031200 Homo sapiens chemokine (C-C motif) receptor 9 (CCR9), transcript variant A

TC107635 NM_031409 Homo sapiens chemokine (C-C motif) receptor 6 (CCR6), transcript variant 2






TC104225 NM_032635 Homo sapiens seven transmembrane domain protein (NIFIE14)


TC108023 NM_033050 Homo sapiens succinate receptor 1 (SUCNR1)

TC123076 NM_033050 Homo sapiens succinate receptor 1 (SUCNR1)

TC124092 NM_033663 Homo sapiens dopamine receptor D3 (DRD3), transcript variant e

TC120211 NM_053278 Homo sapiens trace amine receptor 5 (TRAR5)


TC120221 NM_054030 Homo sapiens G protein-coupled receptor MRGX2 (MRGX2)



TC109187 NM_057159 Homo sapiens endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 2 (EDG2),


TC120259 NM_078481 Homo sapiens CD97 antigen (CD97), transcript variant 1


TC120362 NM_080818 Homo sapiens oxoglutarate (alpha-ketoglutarate) receptor 1 (OXGR1)

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TC100222 NM_144773 Homo sapiens G protein-coupled receptor 73-like 1 (GPR73L1)

TC100046 NM_145015 Homo sapiens MAS-related GPR, member F (MRGPRF)


TC100553 NM_148962 Homo sapiens oxoeicosanoid (OXE) receptor 1 (OXER1)

TC100589 NM_152430 Homo sapiens olfactory receptor, family 51, subfamily E, member 1 (OR51E1)










TC106974 NM_176875 Homo sapiens cholecystokinin B receptor (CCKBR)


TC107310 NM_181657 Homo sapiens leukotriene B4 receptor (LTB4R)

TC107787 XM_049496 Homo sapiens purinergic receptor P2Y G-protein coupled 11 (P2RY11) mRNA

TC112665 XM_051522 Homo sapiens G protein-coupled receptor (RDC1)

TC107081 XM_060958 Homo sapiens similar to seven transmembrane helix receptor (LOC128373)

TC107898 XM_061555 Homo sapiens similar to G protein-coupled receptor 26 (LOC119586)


TC107104 XM_062285 Homo sapiens similar to Olfactory receptor 2D3 (LOC120775)

TC107127 XM_066873 Homo sapiens G-protein coupled receptor 2 (GPCR2)

TC104550 XM_071093 Homo sapiens similar to Olfactory receptor 13C5 (LOC138799)


TC107106 XM_089863 Homo sapiens similar to Olfactory receptor 52I2 (LOC143502)

TC107788 XM_089955 Homo sapiens similar to putative G-protein coupled receptor (LOC159948)

TC107116 XM_096782 Homo sapiens putative leukocyte platelet-activating factor receptor (HUMNPIIY20)

TC107107 XM_210196 Homo sapiens similar to seven transmembrane helix receptor (LOC283193), OriGene unique variant 1

TC107094 XM_291111 PREDICTED: Homo sapiens G protein-coupled receptor 125 (GPR125)

TC107084 XM_291419 Homo sapiens G protein-coupled receptor 153 (GPR153)


TC107109 XM_291977 Homo sapiens similar to Olfactory receptor 52L1 (LOC338751)

TC107089 XM_293092 Homo sapiens G protein-coupled receptor 148 (GPR148)

TC107092 XM_293580 PREDICTED: Homo sapiens G protein-coupled receptor 149 (GPR149)

TC107098 XM_294318 Homo sapiens similar to Olfactory receptor 2A1 (LOC346528)


TC107789 XM_352045 Homo sapiens similar to putative G-protein coupled receptor (LOC376035)

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TC114220 NM_000044 Homo sapiens androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and

bulbar muscular atrophy; Kennedy disease) (AR)

TC123890 NM_000125 Homo sapiens estrogen receptor 1 (ESR1)



TC120032 NM_000176 Homo sapiens nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) (NR3C1)

TC119941 NM_000376 Homo sapiens vitamin D (1,25- dihydroxyvitamin D3) receptor (VDR)

TC123863 NM_000457 Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 2

TC119844 NM_000461 Homo sapiens thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene

homolog 2, avian) (THRB)

TC119849 NM_000475 Homo sapiens nuclear receptor subfamily 0, group B, member 1 (NR0B1)

TC123888 NM_000901 Homo sapiens nuclear receptor subfamily 3, group C, member 2 (NR3C2)

TC123962 NM_000926 Homo sapiens progesterone receptor (PGR)

TC119566 NM_000964 Homo sapiens retinoic acid receptor, alpha (RARA)

TC119567 NM_000965 Homo sapiens retinoic acid receptor, beta (RARB), transcript variant 1

TC119569 NM_000966 Homo sapiens retinoic acid receptor, gamma (RARG)

TC119215 NM_001437 Homo sapiens estrogen receptor 2 (ER beta) (ESR2), OriGene unique variant 1

TC119216 NM_001437 Homo sapiens estrogen receptor 2 (ER beta) (ESR2)

TC119217 NM_001438 Homo sapiens estrogen-related receptor gamma (ESRRG), transcript variant 1

TC119159 NM_001621 Homo sapiens aryl hydrocarbon receptor (AHR)

TC118806 NM_002135 Homo sapiens nuclear receptor subfamily 4, group A, member 1 (NR4A1), transcript variant 1

TC118329 NM_002889 Homo sapiens retinoic acid receptor responder (tazarotene induced) 2 (RARRES2)

TC109741 NM_002943 Homo sapiens RAR-related orphan receptor A (RORA), transcript variant 3

TC109742 NM_002943 Homo sapiens RAR-related orphan receptor A (RORA), OriGene unique variant 1

TC118299 NM_002957 Homo sapiens retinoid X receptor, alpha (RXRA)

TC118079 NM_003250 Homo sapiens thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene

homolog, avian) (THRA), transcript variant 2

TC118080 NM_003250 Homo sapiens thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene

homolog, avian) (THRA), OriGene unique variant 1

TC118089 NM_003269 Homo sapiens nuclear receptor subfamily 2, group E, member 1 (NR2E1)




TC117737 NM_003822 Homo sapiens nuclear receptor subfamily 5, group A, member 2 (NR5A2), OriGene unique variant 1

TC109996 NM_003889 Homo sapiens nuclear receptor subfamily 1, group I, member 2 (NR1I2), OriGene unique variant 1

TC109997 NM_003889 Homo sapiens nuclear receptor subfamily 1, group I, member 2 (NR1I2), transcript variant 1

TC117545 NM_004133 Homo sapiens hepatocyte nuclear factor 4, gamma (HNF4G)

TC108253 NM_004451 Homo sapiens estrogen-related receptor alpha (ESRRA)

TC123865 NM_004452 Homo sapiens estrogen-related receptor beta (ESRRB)

TC117294 NM_004585 Homo sapiens retinoic acid receptor responder (tazarotene induced) 3 (RARRES3)

TC117168 NM_004774 Homo sapiens PPAR binding protein (PPARBP), OriGene unique variant 1

TC116987 NM_005036 Homo sapiens peroxisome proliferative activated receptor, alpha (PPARA), transcript variant 5

TC111129 NM_005122 Homo sapiens nuclear receptor subfamily 1, group I, member 3 (NR1I3)

TC116941 NM_005123 Homo sapiens nuclear receptor subfamily 1, group H, member 4 (NR1H4)

TC101015 NM_005126 Homo sapiens nuclear receptor subfamily 1, group D, member 2 (NR1D2)

TC116862 NM_005234 Homo sapiens nuclear receptor subfamily 2, group F, member 6 (NR2F6)

Nuclear Hormone Receptor CloneSet

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TC122167 AY054985 Homo sapiens voltage-dependent calcium channel beta-4a subunit mRNA, complete cds

TC122546 NM_000079 Homo sapiens cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle) (CHRNA1)

TC122073 NM_000079 Homo sapiens cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle) (CHRNA1)

TC123958 NM_000080 Homo sapiens cholinergic receptor, nicotinic, epsilon polypeptide (CHRNE)

TC122070 NM_000083 Homo sapiens chloride channel 1, skeletal muscle (Thomsen disease, autosomal dominant) (CLCN1)

TC100001 NM_000084 Homo sapiens chloride channel 5 (nephrolithiasis 2, X-linked, Dent disease) (CLCN5)

TC122040 NM_000087 Homo sapiens cyclic nucleotide gated channel alpha 1 (CNGA1)

TC120084 NM_000165 Homo sapiens gap junction protein, alpha 1, 43kDa (connexin 43) (GJA1)

TC120085 NM_000166 Homo sapiens gap junction protein, beta 1, 32kDa (connexin 32, Charcot-Marie-Tooth neuropathy,

X-linked) (GJB1)

TC123992 NM_000171 Homo sapiens glycine receptor, alpha 1 (startle disease/hyperekplexia, stiff man syndrome) (GLRA1),


TC122128 NM_000217 Homo sapiens potassium voltage-gated channel, shaker-related subfamily, member 1 (episodic ataxia

with myokymia) (KCNA1)

TC121930 NM_000219 Homo sapiens potassium voltage-gated channel, Isk-related family, member 1 (KCNE1)

TC122218 NM_000220 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 1 (KCNJ1), transcript

variant rom-k1


variant rom-k1

Ion Channel CloneSet



TC116562 NM_005693 Homo sapiens nuclear receptor subfamily 1, group H, member 3 (NR1H3), OriGene unique variant 1




TC111151 NM_006320 Homo sapiens progesterone receptor membrane component 2 (PGRMC2)

TC115976 NM_006601 Homo sapiens unactive progesterone receptor, 23 kD (TEBP)

TC115946 NM_006667 Homo sapiens progesterone receptor membrane component 1 (PGRMC1)

TC115780 NM_006914 Homo sapiens RAR-related orphan receptor B (RORB)

TC115782 NM_006917 Homo sapiens retinoid X receptor, gamma (RXRG), transcript variant 1




TC114427 NM_016213 Homo sapiens thyroid hormone receptor interactor 4 (TRIP4), OriGene unique variant 1

TC114428 NM_016213 Homo sapiens thyroid hormone receptor interactor 4 (TRIP4), OriGene unique variant 2

TC114329 NM_016346 Homo sapiens nuclear receptor subfamily 2, group E, member 3 (NR2E3), transcript variant 1


TC112578 NM_021724 Homo sapiens nuclear receptor subfamily 1, group D, member 1 (NR1D1)

TC112839 NM_021976 Homo sapiens retinoid X receptor, beta (RXRB)

TC123126 NM_134261 Homo sapiens RAR-related orphan receptor A (RORA), transcript variant 1

TC108192 NM_138711 Homo sapiens peroxisome proliferative activated receptor, gamma (PPARG), transcript variant 3

TC124177 NM_138712 Homo sapiens peroxisome proliferative activated receptor, gamma (PPARG), transcript variant 1

TC109596 NM_177435 Homo sapiens peroxisome proliferative activated receptor, delta (PPARD), transcript variant 2

TC123864 X74497 H.sapiens mRNA for thyroid hormone receptor beta-2 isoform

TC107087 XM_092480 Homo sapiens similar to nuclear receptor subfamily 1, group D, member 1 (LOC151155)

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variant rom-k1

TC123972 NM_000238 Homo sapiens potassium voltage-gated channel, subfamily H (eag-related), member 2 (KCNH2),


TC110976 NM_000297 Homo sapiens polycystic kidney disease 2 (autosomal dominant) (PKD2)

TC124054 NM_000335 Homo sapiens sodium channel, voltage-gated, type V, alpha (long QT syndrome 3) (SCN5A), transcript

variant 2

TC119979 NM_000336 Homo sapiens sodium channel, nonvoltage-gated 1, beta (Liddle syndrome) (SCNN1B)

TC122157 NM_000385 Homo sapiens aquaporin 1 (channel-forming integral protein, 28kDa) (AQP1), transcript variant 2

TC122091 NM_000397 Homo sapiens cytochrome b-245, beta polypeptide (chronic granulomatous disease) (CYBB)

TC123976 NM_000525 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 11 (KCNJ11)

TC124161 NM_000722 Homo sapiens calcium channel, voltage-dependent, alpha 2/delta subunit 1 (CACNA2D1)

TC123891 NM_000723 Homo sapiens calcium channel, voltage-dependent, beta 1 subunit (CACNB1), transcript variant 1



TC122090 NM_000725 Homo sapiens calcium channel, voltage-dependent, beta 3 subunit (CACNB3)

TC119727 NM_000725 Homo sapiens calcium channel, voltage-dependent, beta 3 subunit (CACNB3), OriGene unique variant 1


TC122189 NM_000727 Homo sapiens calcium channel, voltage-dependent, gamma subunit 1 (CACNG1)

TC123917 NM_000742 Homo sapiens cholinergic receptor, nicotinic, alpha polypeptide 2 (neuronal) (CHRNA2)

TC122160 NM_000743 Homo sapiens cholinergic receptor, nicotinic, alpha polypeptide 3 (CHRNA3)


TC108445 NM_000745 Homo sapiens cholinergic receptor, nicotinic, alpha polypeptide 5 (CHRNA5), OriGene unique variant 1



TC122573 NM_000747 Homo sapiens cholinergic receptor, nicotinic, beta polypeptide 1 (muscle) (CHRNB1)

TC122138 NM_000747 Homo sapiens cholinergic receptor, nicotinic, beta polypeptide 1 (muscle) (CHRNB1), OriGene unique

variant 1

TC119699 NM_000748 Homo sapiens cholinergic receptor, nicotinic, beta polypeptide 2 (neuronal) (CHRNB2)

TC122056 NM_000750 Homo sapiens cholinergic receptor, nicotinic, beta polypeptide 4 (CHRNB4), OriGene unique variant 1

TC122142 NM_000750 Homo sapiens cholinergic receptor, nicotinic, beta polypeptide 4 (CHRNB4), OriGene unique variant 2

TC119700 NM_000751 Homo sapiens cholinergic receptor, nicotinic, delta polypeptide (CHRND)

TC119668 NM_000806 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, alpha 1 (GABRA1)






TC122150 NM_000811 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, alpha 6 (GABRA6), OriGene unique variant 1

TC119628 NM_000812 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, beta 1 (GABRB1)

TC119629 NM_000813 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, beta 2 (GABRB2), transcript variant 2


TC122576 NM_000815 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, delta (GABRD)

TC109261 NM_000816 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, gamma 2 (GABRG2), transcript variant 2

TC119636 NM_000824 Homo sapiens glycine receptor, beta (GLRB)

TC119637 NM_000826 Homo sapiens glutamate receptor, ionotropic, AMPA 2 (GRIA2)

TC119638 NM_000827 Homo sapiens glutamate receptor, ionotropic, AMPA 1 (GRIA1)

TC122080 NM_000828 Homo sapiens glutamate receptor, ionotrophic, AMPA 3 (GRIA3), transcript variant flop

TC119639 NM_000829 Homo sapiens glutamate receptor, ionotrophic, AMPA 4 (GRIA4)

33ph: 1.888.267.4436 fax: 1.301.340.9254

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TC111634 NM_000830 Homo sapiens glutamate receptor, ionotropic, kainate 1 (GRIK1), OriGene unique variant 1

TC122079 NM_000830 Homo sapiens glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 1



TC119642 NM_000834 Homo sapiens glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B)

TC122578 NM_000869 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 3A (HTR3A), transcript variant 2



TC121957 NM_001002914 Homo sapiens potassium channel tetramerisation domain containing 11 (KCTD11)

TC119540 NM_001037 Homo sapiens sodium channel, voltage-gated, type I, beta (SCN1B), transcript variant a

TC119542 NM_001038 Homo sapiens sodium channel, nonvoltage-gated 1 alpha (SCNN1A)

TC119543 NM_001039 Homo sapiens sodium channel, nonvoltage-gated 1, gamma (SCNN1G)

TC124011 NM_001094 Homo sapiens amiloride-sensitive cation channel 1, neuronal (degenerin) (ACCN1), transcript variant 2

TC108885 NM_001095 Homo sapiens amiloride-sensitive cation channel 2, neuronal (ACCN2), transcript variant 2

TC123906 NM_001285 Homo sapiens chloride channel, calcium activated, family member 1 (CLCA1)


TC122134 NM_001286 Homo sapiens chloride channel 6 (CLCN6), transcript variant ClC-6a

TC124176 NM_001286 Homo sapiens chloride channel 6 (CLCN6), transcript variant ClC-6a

TC108053 NM_001287 Homo sapiens chloride channel 7 (CLCN7)

TC122065 NM_001287 Homo sapiens chloride channel 7 (CLCN7), OriGene unique variant 1

TC119324 NM_001288 Homo sapiens chloride intracellular channel 1 (CLIC1)


TC122039 NM_001289 Homo sapiens chloride intracellular channel 2 (CLIC2), OriGene unique variant 1

TC119328 NM_001293 Homo sapiens chloride channel, nucleotide-sensitive, 1A (CLNS1A)

TC119289 NM_001298 Homo sapiens cyclic nucleotide gated channel alpha 3 (CNGA3), OriGene unique variant 1

TC123987 NM_001510 Homo sapiens glutamate receptor, ionotropic, delta 2 (GRID2)

TC121946 NM_001680 Homo sapiens FXYD domain containing ion transport regulator 2 (FXYD2), transcript variant a

TC110856 NM_001829 Homo sapiens chloride channel 3 (CLCN3), transcript variant b


TC123926 NM_002042 Homo sapiens gamma-aminobutyric acid (GABA) receptor, rho 1 (GABRR1), OriGene unique variant 1

TC123929 NM_002043 Homo sapiens gamma-aminobutyric acid (GABA) receptor, rho 2 (GABRR2)


TC110908 NM_002063 Homo sapiens glycine receptor, alpha 2 (GLRA2)

TC122177 NM_002063 Homo sapiens glycine receptor, alpha 2 (GLRA2), OriGene unique variant 1

TC120401 NM_002088 Homo sapiens glutamate receptor, ionotropic, kainate 5 (GRIK5)

TC118765 NM_002232 Homo sapiens potassium voltage-gated channel, shaker-related subfamily, member 3 (KCNA3)


TC123963 NM_002234 Homo sapiens potassium voltage-gated channel, shaker-related subfamily, member 5 (KCNA5),




TC118767 NM_002236 Homo sapiens potassium voltage-gated channel, subfamily F, member 1 (KCNF1)

TC121929 NM_002237 Homo sapiens potassium voltage-gated channel, subfamily G, member 1 (KCNG1), transcript variant 1




TC101096 NM_002242 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 13 (KCNJ13), OriGene

unique variant 1

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unique variant 2


variant 2

TC110937 NM_002245 Homo sapiens potassium channel, subfamily K, member 1 (KCNK1)


TC122141 NM_002247 Homo sapiens potassium large conductance calcium-activated channel, subfamily M, alpha member 1

(KCNMA1), OriGene unique variant 3





TC124003 NM_002248 Homo sapiens potassium intermediate/small conductance calcium-activated channel, subfamily N,

member 1 (KCNN1)


member 4 (KCNN4), OriGene unique variant 1


member 4 (KCNN4)

TC118715 NM_002252 Homo sapiens potassium voltage-gated channel, delayed-rectifier, subfamily S, member 3 (KCNS3)

TC118594 NM_002558 Homo sapiens purinergic receptor P2X, ligand-gated ion channel, 1 (P2RX1)

TC123980 NM_002559 Homo sapiens purinergic receptor P2X, ligand-gated ion channel, 3 (P2RX3)

TC122124 NM_002560 Homo sapiens purinergic receptor P2X, ligand-gated ion channel, 4 (P2RX4), transcript variant 1

TC121945 NM_002560 Homo sapiens purinergic receptor P2X, ligand-gated ion channel, 4 (P2RX4), OriGene unique

variant 1



TC122064 NM_002562 Homo sapiens purinergic receptor P2X, ligand-gated ion channel, 7 (P2RX7), OriGene unique variant 1

TC118315 NM_002978 Homo sapiens sodium channel, nonvoltage-gated 1, delta (SCNN1D), OriGene unique variant 1

TC122116 NM_002978 Homo sapiens sodium channel, nonvoltage-gated 1, delta (SCNN1D), OriGene unique variant 2

TC122139 NM_002978 Homo sapiens sodium channel, nonvoltage-gated 1, delta (SCNN1D)

TC121934 NM_003304 Homo sapiens transient receptor potential cation channel, subfamily C, member 1 (TRPC1)

TC124002 NM_003305 Homo sapiens transient receptor potential cation channel, subfamily C, member 3 (TRPC3), OriGene

unique variant 1

TC123988 NM_003374 Homo sapiens voltage-dependent anion channel 1 (VDAC1)



TC123984 NM_003471 Homo sapiens potassium voltage-gated channel, shaker-related subfamily, beta member 1 (KCNAB1),



TC122666 NM_004004 Homo sapiens gap junction protein, beta 2, 26kDa (connexin 26) (GJB2)

TC117547 NM_004137 Homo sapiens potassium large conductance calcium-activated channel, subfamily M, beta member 1

(KCNMB1)

TC122132 NM_004183 Homo sapiens vitelliform macular dystrophy (Best disease, bestrophin) (VMD2), OriGene unique variant 1



TC122684 NM_004588 Homo sapiens sodium channel, voltage-gated, type II, beta (SCN2B)

TC122148 NM_004621 Homo sapiens transient receptor potential cation channel, subfamily C, member 6 (TRPC6), OriGene

unique variant 1



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TC111730 NM_004769 Homo sapiens amiloride-sensitive cation channel 3 (ACCN3), transcript variant 1


TC124000 NM_004961 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, epsilon (GABRE), OriGene unique

variant 1

TC117021 NM_004975 Homo sapiens potassium voltage-gated channel, Shab-related subfamily, member 1 (KCNB1)

TC124010 NM_004976 Homo sapiens potassium voltage-gated channel, Shaw-related subfamily, member 1 (KCNC1)

TC111648 NM_004978 Homo sapiens potassium voltage-gated channel, Shaw-related subfamily, member 4 (KCNC4),


TC122175 NM_004980 Homo sapiens potassium voltage-gated channel, Shal-related subfamily, member 3 (KCND3), OriGene

unique variant 1


variant 2



TC121958 NM_005031 Homo sapiens FXYD domain containing ion transport regulator 1 (phospholemman) (FXYD1),

transcript variant a

TC122072 NM_005199 Homo sapiens cholinergic receptor, nicotinic, gamma polypeptide (CHRNG)

TC123931 NM_005267 Homo sapiens gap junction protein, alpha 8, 50kDa (connexin 50) (GJA8), OriGene unique variant 1

TC124154 NM_005268 Homo sapiens gap junction protein, beta 5 (connexin 31.1) (GJB5)

TC123978 NM_005446 Homo sapiens purinergic receptor P2X-like 1, orphan receptor (P2RXL1), OriGene unique variant 1




TC116623 NM_005662 Homo sapiens voltage-dependent anion channel 3 (VDAC3), OriGene unique variant 1


TC121947 NM_005845 Homo sapiens ATP-binding cassette, sub-family C (CFTR/MRP), member 4 (ABCC4)

TC124178 NM_005845 Homo sapiens ATP-binding cassette, sub-family C (CFTR/MRP), member 4 (ABCC4)

TC116402 NM_005971 Homo sapiens FXYD domain containing ion transport regulator 3 (FXYD3), transcript variant 1

TC124025 NM_006030 Homo sapiens calcium channel, voltage-dependent, alpha 2/delta subunit 2 (CACNA2D2), transcript

variant 2


TC116336 NM_006114 Homo sapiens translocase of outer mitochondrial membrane 40 homolog (yeast) (TOMM40)



TC123944 NM_007052 Homo sapiens NADPH oxidase 1 (NOX1), transcript variant NOH-1L

TC123959 NM_007052 Homo sapiens NADPH oxidase 1 (NOX1), transcript variant NOH-1L

TC115601 NM_007327 Homo sapiens glutamate receptor, ionotropic, N-methyl D-aspartate 1 (GRIN1), transcript variant NR1-3

TC124028 NM_007332 Homo sapiens transient receptor potential cation channel, subfamily A, member 1 (TRPA1)



TC115436 NM_012281 Homo sapiens potassium voltage-gated channel, Shal-related subfamily, member 2 (KCND2)

TC122784 NM_012282 Homo sapiens KCNE1-like (KCNE1L)

TC122179 NM_012283 Homo sapiens potassium voltage-gated channel, subfamily G, member 2 (KCNG2), OriGene unique

variant 1

TC122037 NM_012283 Homo sapiens potassium voltage-gated channel, subfamily G, member 2 (KCNG2)

TC122182 NM_012285 Homo sapiens potassium voltage-gated channel, subfamily H (eag-related), member 4 (KCNH4)

TC111208 NM_012329 Homo sapiens monocyte to macrophage differentiation-associated (MMD)

TC110362 NM_012417 Homo sapiens phosphatidylinositol transfer protein, cytoplasmic 1 (PITPNC1), transcript variant 1

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TC115134 NM_014211 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, pi (GABRP)


TC115023 NM_014379 Homo sapiens potassium channel, subfamily V, member 1 (KCNV1)


TC123934 NM_014407 Homo sapiens potassium large conductance calcium-activated channel, subfamily M beta member 3

(KCNMB3), OriGene unique variant 1


(KCNMB3), transcript variant 4

TC108188 NM_014505 Homo sapiens potassium large conductance calcium-activated channel, subfamily M, beta member 4

(KCNMB4)

TC114946 NM_014592 Homo sapiens Kv channel interacting protein 1 (KCNIP1)

TC111199 NM_015127 Homo sapiens Mid-1-related chloride channel 1 (MCLC), OriGene unique variant 1

TC122186 NM_015127 Homo sapiens Mid-1-related chloride channel 1 (MCLC), OriGene unique variant 2

TC122089 NM_015127 Homo sapiens Mid-1-related chloride channel 1 (MCLC)

TC121941 NM_015532 Homo sapiens glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (GRINL1A)

TC122109 NM_015532 Homo sapiens glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (GRINL1A), OriGene

unique variant 1

TC111264 NM_015993 Homo sapiens transmembrane 4 superfamily member 11 (plasmolipin) (TM4SF11)

TC122840 NM_016112 Homo sapiens polycystic kidney disease 2-like 1 (PKD2L1)

TC122207 NM_016112 Homo sapiens polycystic kidney disease 2-like 1 (PKD2L1)

TC114461 NM_016113 Homo sapiens transient receptor potential cation channel, subfamily V, member 2 (TRPV2)



TC114228 NM_016582 Homo sapiens solute carrier family 15, member 3 (SLC15A3)

TC122061 NM_016611 Homo sapiens potassium channel, subfamily K, member 4 (KCNK4), transcript variant 1


TC122137 NM_017419 Homo sapiens amiloride-sensitive cation channel 5, intestinal (ACCN5)


TC123991 NM_017636 Homo sapiens transient receptor potential cation channel, subfamily M, member 4 (TRPM4)

TC122209 NM_017901 Homo sapiens two pore segment channel 1 (TPCN1)

TC122156 NM_017901 Homo sapiens two pore segment channel 1 (TPCN1), OriGene unique variant 1

TC124160 NM_018298 Homo sapiens mucolipin 3 (MCOLN3)

TC113489 NM_018398 Homo sapiens calcium channel, voltage-dependent, alpha 2/delta 3 subunit (CACNA2D3), OriGene

unique variant 1

TC121942 NM_018398 Homo sapiens calcium channel, voltage-dependent, alpha 2/delta 3 subunit (CACNA2D3)

TC113490 NM_018400 Homo sapiens sodium channel, voltage-gated, type III, beta (SCN3B)

TC122106 NM_018400 Homo sapiens sodium channel, voltage-gated, type III, beta (SCN3B), OriGene unique variant 1



unique variant 1

TC122197 NM_018674 Homo sapiens amiloride-sensitive cation channel 4, pituitary (ACCN4), transcript variant 1

TC122126 NM_018727 Homo sapiens transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript

variant 2


variant 2


variant 2

TC113285 NM_018992 Homo sapiens potassium channel tetramerisation domain containing 5 (KCTD5), OriGene unique

variant 1

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TC122099 NM_019842 Homo sapiens potassium voltage-gated channel, KQT-like subfamily, member 5 (KCNQ5)

TC122113 NM_020039 Homo sapiens amiloride-sensitive cation channel 2, neuronal (ACCN2), transcript variant 1



TC122118 NM_020435 Homo sapiens gap junction protein, alpha 12, 47kDa (GJA12)

TC122214 NM_020659 Homo sapiens tweety homolog 1 (Drosophila) (TTYH1), OriGene unique variant 1

TC122104 NM_020660 Homo sapiens connexin-36 (CX36)

TC121938 NM_020897 Homo sapiens hyperpolarization activated cyclic nucleotide-gated potassium channel 3 (HCN3)

TC121953 NM_020952 Homo sapiens transient receptor potential cation channel, subfamily M, member 3 (TRPM3),



TC122166 NM_021072 Homo sapiens hyperpolarization activated cyclic nucleotide-gated potassium channel 1 (HCN1)

TC112982 NM_021137 Homo sapiens tumor necrosis factor, alpha-induced protein 1 (endothelial) (TNFAIP1)




TC109579 NM_021902 Homo sapiens FXYD domain containing ion transport regulator 1 (phospholemman) (FXYD1), transcript

variant b


TC122077 NM_021912 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, beta 3 (GABRB3), OriGene unique

variant 1

TC123942 NM_021954 Homo sapiens gap junction protein, alpha 3, 46kDa (connexin 46) (GJA3), OriGene unique variant 1


TC123967 NM_021984 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, epsilon (GABRE), transcript variant 2

TC122169 NM_022003 Homo sapiens FXYD domain containing ion transport regulator 6 (FXYD6), OriGene unique variant 1

TC112567 NM_022003 Homo sapiens FXYD domain containing ion transport regulator 6 (FXYD6)


TC112856 NM_022054 Homo sapiens potassium channel, subfamily K, member 13 (KCNK13), OriGene unique variant 1


TC122205 NM_022358 Homo sapiens potassium channel, subfamily K, member 15 (KCNK15), OriGene unique variant 1


TC112319 NM_024009 Homo sapiens gap junction protein, beta 3, 31kDa (connexin 31) (GJB3), transcript variant 1

TC112300 NM_024047 Homo sapiens nudix (nucleoside diphosphate linked moiety X)-type motif 9 (NUDT9), transcript variant 1


variant 1


TC122185 NM_024080 Homo sapiens transient receptor potential cation channel, subfamily M, member 8 (TRPM8), OriGene

unique variant 1

TC112270 NM_024080 Homo sapiens transient receptor potential cation channel, subfamily M, member 8 (TRPM8)

TC123954 NM_024505 Homo sapiens NADPH oxidase, EF hand calcium-binding domain 5 (NOX5)


TC111844 NM_025221 Homo sapiens Kv channel interacting protein 4 (KCNIP4), transcript variant 1








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TC122107 NM_031896 Homo sapiens calcium channel, voltage-dependent, gamma subunit 7 (CACNG7), OriGene unique

variant 1


variant 1



variant 2


TC101325 NM_033223 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, gamma 3 (GABRG3), OriGene unique

variant 1

TC122076 NM_033223 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, gamma 3 (GABRG3)

TC122210 NM_033347 Homo sapiens potassium channel, subfamily K, member 7 (KCNK7), transcript variant A

TC111509 NM_054020 Homo sapiens cation channel, sperm associated 2 (CATSPER2), transcript variant 1


TC122110 NM_130770 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 3, family member C (HTR3C), OriGene unique

variant 2

TC122044 NM_130770 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 3, family member C (HTR3C), OriGene unique

variant 1

TC122066 NM_133445 Homo sapiens glutamate receptor, ionotropic, N-methyl-D-aspartate 3A (GRIN3A)

TC123125 NM_133490 Homo sapiens potassium voltage-gated channel, subfamily G, member 4 (KCNG4), transcript variant 2

TC123935 NM_133497 Homo sapiens potassium channel, subfamily V, member 2 (KCNV2)



TC122216 NM_138392 Homo sapiens SH3KBP1 binding protein 1 (SHKBP1)



TC122042 NM_139075 Homo sapiens two pore segment channel 2 (TPCN2)


TC109363 NM_139137 Homo sapiens potassium voltage-gated channel, Shaw-related subfamily, member 2 (KCNC2), transcript

variant 2

TC120679 NM_139202 Homo sapiens megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant 2



TC122054 NM_139320 Homo sapiens CHRNA7 (cholinergic receptor, nicotinic, alpha polypeptide 7, exons 5-10) and FAM7A

(family with sequence similarity 7A, exons A-E) fusion (CHRFAM7A), transcript variant 1

TC124168 NM_139320 Homo sapiens CHRNA7 (cholinergic receptor, nicotinic, alpha polypeptide 7, exons 5-10) and FAM7A

(family with sequence similarity 7A, exons A-E) fusion (CHRFAM7A), transcript variant 1

TC100185 NM_144633 Homo sapiens potassium voltage-gated channel, subfamily H (eag-related), member 8 (KCNH8)



TC123234 NM_145814 Homo sapiens calcium channel, voltage-dependent, gamma subunit 6 (CACNG6), transcript variant 1



TC122145 NM_147181 Homo sapiens Kv channel interacting protein 4 (KCNIP4), OriGene unique variant 1



variant 2


TC122063 NM_152387 Homo sapiens hypothetical protein FLJ31322 (FLJ31322), OriGene unique variant 1

TC100666 NM_152439 Homo sapiens vitelliform macular dystrophy 2-like 3 (VMD2L3)

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variant 1




TC122213 NM_153274 Homo sapiens vitelliform macular dystrophy 2-like 2 (VMD2L2), OriGene unique variant 1


TC109364 NM_153748 Homo sapiens potassium voltage-gated channel, Shaw-related subfamily, member 2 (KCNC2),



variant rom-k3


variant rom-k5


variant rom-k5



variant 2


variant 3















TC122127 NM_172097 Homo sapiens cation channel, sperm associated 2 (CATSPER2), OriGene unique variant 1

TC122172 NM_172097 Homo sapiens cation channel, sperm associated 2 (CATSPER2), OriGene unique variant 2

TC122047 NM_172109 Homo sapiens potassium voltage-gated channel, KQT-like subfamily, member 2 (KCNQ2), transcript

variant 5







TC123965 NM_172364 Homo sapiens calcium channel, voltage-dependent, alpha 2/delta subunit 4 (CACNA2D4), transcript

variant 1









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TC101135 NM_173536 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, gamma 1 (GABRG1)

TC124180 NM_173562 Homo sapiens chromosome 6 open reading frame 69 (C6orf69)

TC122121 NM_173562 Homo sapiens chromosome 6 open reading frame 69 (C6orf69), OriGene unique variant 2

TC122096 NM_173562 Homo sapiens chromosome 6 open reading frame 69 (C6orf69), OriGene unique variant 1

TC122103 NM_173872 Homo sapiens chloride channel 3 (CLCN3), OriGene unique variant 1


TC123982 NM_174934 Homo sapiens sodium channel, voltage-gated, type IV, beta (SCN4B)





TC123945 NM_178019 Homo sapiens cation channel, sperm associated 3 (CATSPER3)


TC122050 NM_180990 Homo sapiens ligand-gated ion channel subunit (LGICZ)

TC122192 NM_181671 Homo sapiens phosphatidylinositol transfer protein, cytoplasmic 1 (PITPNC1), transcript variant 2

TC122130 NM_181894 Homo sapiens glutamate receptor, ionotrophic, AMPA 3 (GRIA3), OriGene unique variant 1

TC107729 NM_198039 Homo sapiens nudix (nucleoside diphosphate linked moiety X)-type motif 9 (NUDT9), transcript variant 2

TC121943 NM_198098 Homo sapiens aquaporin 1 (channel-forming integral protein, 28kDa) (AQP1), transcript variant 1


TC122075 NM_198991 Homo sapiens potassium channel tetramerisation domain containing 1 (KCTD1), OriGene unique variant 1




TC122135 NM_201572 Homo sapiens calcium channel, voltage-dependent, beta 2 subunit (CACNB2), OriGene unique variant 1

TC123990 XM_029962 PREDICTED: Homo sapiens potassium channel, subfamily T, member 1 (KCNT1)

TC121935 XM_043613 PREDICTED: Homo sapiens glutamate receptor, ionotropic, delta 1 (GRID1)

TC122041 XM_086565 Homo sapiens hyperpolarization activated cyclic nucleotide-gated potassium channel 3 (HCN3)

TC122071 XM_086565 Homo sapiens hyperpolarization activated cyclic nucleotide-gated potassium channel 3 (HCN3), OriGene

unique variant 1

TC121931 XM_291095 PREDICTED: Homo sapiens similar to glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A

(LOC339970)

TC123930 XM_371263 PREDICTED: Homo sapiens mucolipin 2 (MCOLN2)

TC122173 XR_000147 PREDICTED: Homo sapiens transient receptor potential cation channel, subfamily C, member 2

(TRPC2), misc RNA

P450 Clone SetCat. No. Accession No. Description

TC102224 NM_000102 Homo sapiens cytochrome P450, family 17, subfamily A, polypeptide 1 (CYP17A1)

TC103335 NM_000104 Homo sapiens cytochrome P450, family 1, subfamily B, polypeptide 1 (CYP1B1)

TC104446 NM_000106 Homo sapiens cytochrome P450, family 2, subfamily D, polypeptide 6 (CYP2D6)

TC120793 NM_000106 Homo sapiens cytochrome P450, family 2, subfamily D, polypeptide 6 (CYP2D6), OriGene unique

variant 1

TC123879 NM_000497 Homo sapiens cytochrome P450, family 11, subfamily B, polypeptide 1 (CYP11B1), nuclear gene

encoding mitochondrial protein


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TC119859 NM_000500 Homo sapiens cytochrome P450, family 21, subfamily A, polypeptide 2 (CYP21A2), OriGene unique

variant 1



TC119672 NM_000762 Homo sapiens cytochrome P450, family 2, subfamily A, polypeptide 6 (CYP2A6), OriGene unique variant 1


TC120800 NM_000764 Homo sapiens cytochrome P450, family 2, subfamily A, polypeptide 7 (CYP2A7), transcript variant 1




TC119677 NM_000771 Homo sapiens cytochrome P450, family 2, subfamily C, polypeptide 9 (CYP2C9)

TC120796 NM_000772 Homo sapiens cytochrome P450, family 2, subfamily C, polypeptide 18 (CYP2C18)

TC119678 NM_000773 Homo sapiens cytochrome P450, family 2, subfamily E, polypeptide 1 (CYP2E1)

TC120795 NM_000774 Homo sapiens cytochrome P450, family 2, subfamily F, polypeptide 1 (CYP2F1), OriGene unique variant 1


TC119679 NM_000775 Homo sapiens cytochrome P450, family 2, subfamily J, polypeptide 2 (CYP2J2)





TC120788 NM_000779 Homo sapiens cytochrome P450, family 4, subfamily B, polypeptide 1 (CYP4B1), OriGene unique variant 1


TC119684 NM_000781 Homo sapiens cytochrome P450, family 11, subfamily A, polypeptide 1 (CYP11A1), nuclear gene encoding





TC119685 NM_000784 Homo sapiens cytochrome P450, family 27, subfamily A, polypeptide 1 (CYP27A1), nuclear gene encoding


TC123873 NM_000785 Homo sapiens cytochrome P450, family 27, subfamily B, polypeptide 1 (CYP27B1), nuclear gene encoding



TC119595 NM_000896 Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 3 (CYP4F3)

TC120817 NM_000917 Homo sapiens procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha

polypeptide I (P4HA1), OriGene unique variant 1

TC110967 NM_000917 Homo sapiens procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha

polypeptide I (P4HA1)

TC100401 NM_000941 Homo sapiens P450 (cytochrome) oxidoreductase (POR)

TC123875 NM_000961 Homo sapiens prostaglandin I2 (prostacyclin) synthase (PTGIS)

TC109823 NM_001061 Homo sapiens thromboxane A synthase 1 (platelet, cytochrome P450, family 5, subfamily A) (TBXAS1),

transcript variant TXS-I









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TC120787 NM_017781 Homo sapiens cytochrome P450, family 2, subfamily W, polypeptide 1 (CYP2W1)


TC120809 NM_019885 Homo sapiens cytochrome P450, family 26, subfamily B, polypeptide 1 (CYP26B1), OriGene unique variant 1




TC120811 NM_024514 Homo sapiens cytochrome P450, family 2, subfamily R, polypeptide 1 (CYP2R1)

TC109968 NM_030622 Homo sapiens cytochrome P450, family 2, subfamily S, polypeptide 1 (CYP2S1), OriGene unique variant 1

TC109139 NM_030878 Homo sapiens cytochrome P450, family 2, subfamily C, polypeptide 8 (CYP2C8), OriGene unique

variant 1p1-2

TC120816 NM_030878 Homo sapiens cytochrome P450, family 2, subfamily C, polypeptide 8 (CYP2C8), transcript variant Hp1-2



TC107078 NM_178033 Homo sapiens cytochrome P450, family 4, subfamily X, polypeptide 1 (CYP4X1), OriGene unique variant 1

TC120798 NM_178033 Homo sapiens cytochrome P450, family 4, subfamily X, polypeptide 1 (CYP4X1)

TC123874 NM_178134 Homo sapiens cytochrome P450, family 4, subfamily Z, polypeptide 1 (CYP4Z1)

TC120792 NM_183075 Homo sapiens cytochrome P450, family 2, subfamily U, polypeptide 1 (CYP2U1)

TC106908 NR_001278 Homo sapiens cytochrome P450, family 2, subfamily B, polypeptide 7 pseudogene 1 (CYP2B7P1) on

chromosome 19

TC120791 XM_208213 PREDICTED: Homo sapiens cytochrome P450, family 4, subfamily A, polypeptide 22 (CYP4A22)

TC120790 XM_209612 Homo sapiens hypothetical protein LOC285440 (LOC285440)

TC123886 XM_291005 Homo sapiens similar to 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial precursor

(Calcidiol 1-monooxygenase) (25-OHD-1 alpha-hydroxylase) (25-hydroxyvitamin D(3) 1-alpha-hydroxylase)

(VD3 1A hydroxylase) (P450C1 alpha) (P450VD1-alpha) (LOC339761)

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HuSH™Premade shRNA expression panels

Kinase shRNAs

Nuclear hormone receptor shRNAs

Signal molecule shRNAs

pRS vectors

1. Unlocking the potential of the human genome with RNA interference.Nature. 2004 Sep 16;431(7006):371-8.

2. RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23nucleotide intervals.Cell. 2000 Mar 31;101(1):25-33.

3. siRNAs: applications in functional genomics and potential as therapeutics.Nat Rev Drug Discov. 2004 Apr;3(4):318-29.

4. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells.Nature. 2001 May 24;411(6836):494-8.

5. A system for stable expression of short interfering RNAs in mammalian cells.Science. 2002 Apr 19;296(5567):550-3.

6. Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells.Genes Dev. 2002 Apr 15;16(8):948-58.

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Features Plasmid based vectors Synthetic siRNA

Antibiotic resistance for selection Yes No

Monitor cell transfection efficiency Yes No

Cost per gene Moderate High

Gene inhibition studies Long term Short term

Stable cell line production Yes No

Effective delivery of siRNA into hard to transfect cells Yes No

Introduction:

As a cellular defense mechanism, host cells process

double-stranded RNA into small interfering RNA

(siRNA), which then targets homologous RNAs for

destruction1. RNAi occurs through a series of steps

involving the generation of small interfering RNAs

(siRNAs) in vivo via the action of a specific RNAase III

endonuclease Dicer.The resulting siRNAs mediate the

degradation of their complementary RNA by

association of the siRNA with a nuclease complex to

form what is called the RNA-Induced Silencing

Complex (RISC). In the next step, an unwinding of the

siRNA occurs which activates RISC. It is the activated

RISC that binds to the target mRNA and finally leads

to the loss of expression of the gene it coded2.

RNAi technology can be implemented mainly with

either small interference double stranded RNA

(siRNA) or short hairpin RNA (shRNA). Experiments

have indicated that both siRNA and shRNA are also

far more potent than hammerhead ribosome

ribozymes based approaches3. In mammalian cells,

RNA interference (RNAi) can be triggered by 21-

base-pair siRNA that causes strong, yet transient,

inhibition of gene expression on specific genes4.

These siRNAs can be generated in vitro and

transfected into mammalian cells, resulting in

effective suppression of the intended genes.

Unfortunately, such suppression is typically

transient. By contrast, vector-expressing short

hairpin RNA (shRNA) can suppress gene expression

over a prolonged period(5,6). The unique advantages

of the plasmid-based shRNA approach are listed

below.

HuSH™—Premade shRNA

expression panels

Product Listing Cat. No. PriceHuSH™ shRNA panels TRXXXXXX 380

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Product Description

What are HuSH™ shRNA expression plasmids?HuSH plasmids were designed to effectively express

the insert DNA sequences in transfected mammalian

cells. OriGene gene-specific silencing plasmids

contain a U6 promoter, 21 base pair (bp) gene-

specific sequences, a 10 bp intervening sequence,

another 21 base pair gene-specific reverse

complement sequence, and a transcription termi-

nation sequence. The expression of the insert

sequence in mammalian cells results in the

formation of a short hairpin RNA with a 21 bp stem

and a 10-nucleotide loop. Such a structure has been

shown to be able to effectively knock-down the

expression of target genes.

OriGene’s HuSH plasmids have been shown to

knock-down the expression of green fluorescence

protein, luciferase and HER2 oncogene. On

average, more than 25% of the shRNA tested are

capable of inhibiting the target genes by 70% or

more. In addition, the pRS plasmid contains a

puromycin selectable marker, and a pair of retro-

viral integration sites flanking the gene silencing

cassette and the selection marker. Both the

selection marker and the retroviral integration

functions have been validated.

HuSH plasmids utilize the most commonly used

human U6 promoter to express the shRNA. A poly-T

track is added immediately downstream of the hairpin

sequence to ensure the termination of transcription

following the hairpin sequences. The resulting shRNA

is to be processed by Dicers, and incorporated into

RISC complexes. Once in the RISC complexes, they

will find the target mRNA through specific sequence

recognition and cleave the target mRNA.

With the HuSH shRNA expression vectors, shRNA

(short-hairpin RNA) can be expressed continuously

in human cells to achieve stable and reliable gene

ADVANTAGES

Ready-to-transfect constructs

Multiple vectors to achieve optimized/dosed inhibition

PuromycinR marker for selection

Enable stable cell line production

More effective delivery

Non-exhaustible supply of DNA

shRNA oligos

pBS Ori

pRS5522 bp

U6 promoter

Ampicillin

SV40 early promoter

Puro

3‘ LTR5‘ LTR

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silencing. By establishing stable cell lines with

effective shRNA expression vectors, one can perform

long term gene silencing studies, eliminating the

need to synthesize siRNA, repeat transfection, and

validate, as well as eliminating the experimental

variation frequently associated with siRNA.

The HuSH backbone pRS vector (Fig. 1):

• Designed for long-term gene silencing studies

• MMLV LTR sequences for establishing stable cell

lines via retroviruses

• Puromycin resistant marker for easy selection of

infected or transfected cells

• U6 polymerase III promoter for shRNA expression

Why should I buy a HuSH mammalianshRNA expression plasmid rather than a synthetic siRNA?

HuSH plasmids offer researchers an alternative to

inhibiting target genes. There are several advantages

to this expression plasmid based approach:

1. Long-term gene silencing:

The length of gene silencing can be longer as the

plasmid continuously expresses the shRNA of the

plasmid. In contrast, synthetic siRNAs either get

diluted during cell division or are degraded.

2. Drug-selectivity:

When the transfection efficiency is limited, one can

select plasmid-transfected cells with puromycin to

ensure efficient gene silencing.

3. Retroviral capacity:

One can obtain stable cell lines either through plas-

mid transfection or viral infection via a packaging

cell line.

4. Inexhaustible supply:

The plasmids can be amplified and thus represent

an unlimited source for future experiments.

What is the U6 promoter?

The pRS plasmid contains the human U6 promoter

that has been used extensively to express shRNAs in

mammalian cells through RNA Poll III. This promoter

Fig. 1

pRS plasmid map. The shRNA oligos will be cloned between

BamHI and BbsI, immediately downstream of U6 promoter. A

puromycin selection marker is present. Both 5’ and 3’ LTRs are

from MMLV and used to generate retroviruses in appropriate

packaging cells.

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has been validated in the HuSH plasmid via gene

knock-down experiments.

What is the gene specific shDNA inserted downstream of U6 promoter?The shDNA inserts were generated directly from the

cDNA of the target genes through a random

restriction enzyme digestion approach. All shRNA

expression cassette sequences were verified via

DNA sequence analysis and matched against the

target gene sequences. Both the sequences and the

corresponding locations of the target genes are

provided.

How does the transcription terminate?A stretch of 5 thymidine nucleotides (dT5) is attached

immediately after the shRNA sequences. This dT5

leads to transcription termination immediately

following the shRNA sequences.

How does OriGene verify the shDNAsequences for specific genes?All shRNA sequences in the expression vectors were

verified through DNA sequence analysis and

matched against the target genes.

Why does OriGene provide up to 8 shRNA expression vectors per gene?Based on experiments conducted at OriGene,

approximately 25-30% of the shRNA vectors resulted

in >70% inhibition of the target genes. Testing 8

shRNA expression vectors virtually ensures that one

can obtain at least one functional shRNA vector.

How should I use the products?OriGene recommends that researchers first transfect

an aliquot of each of the supplied plasmids into

competent E. coli cells and perform a DNA mini-prep.

The plasmids can then be transfected into a target

cell line with FuGene6. Three days after transfection,

cell lysates can be obtained and used for western

blot analysis using an antibody against the target

protein to verify the functionality of the shRNA

vectors.

How do I choose the right shRNAexpression vector for gene silencing?The functionally active shRNA expression plasmids

need to be selected experimentally in transiently

transfected cells.

Can I select for the pRS plasmidtransfected cells?Yes. Two days after transfection, the cells can be

removed from the plates via trypsin, plated and

selected out with culture media containing

puromycin (0.5 ug/ml).

How can I create a stable cell line with afunctional shRNA expression vector? Stable cell lines can be generated using two different

approaches. First, target HEK cells can be transfected

with a functionally validated shRNA plasmid. Two

days after transfection, the transfected HEK cells can

be selected with 0.5 ug/ml puromycin over one week

with passages as needed. Alternatively, retroviral

packaging cell lines can be used to generate

retroviruses for stable cell line generation. In one

example, the plasmid can be transiently transfected

into a Phoenix helper free amphotropic packaging

cells line (http://www.orbigen.com/). Two days after

transfection, the cell culture supernatant is collected,

filtered, and used to infect the target cell lines. Stable

cell lines can be established following drug selection.

For what purpose is the empty pRS-Con used?For shRNA experiments, it is important to demonstrate

that the effect of a specific shRNA expression vector is

gene specific, and is not due to the non-specific effect

known as interferon response. pRS-Con was designed

for this purpose; the inserted cassette contains a

sequence from green fluorescence protein, unrelated

to any human expressed sequence.

What does OriGene guarantee with re-gards to its shRNA expression plasmids?OriGene guarantees all shRNA expression cassettes

are sequence verified. The pRS expression plasmid

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vectors have been tested for producing shRNA in

gene silencing studies against green fluorescence

protein, luciferase and human HER2 oncogene.

Product Application:

HuSH plasmids:

Currently HuSH products are focused on

• Protein kinases

• Nuclear hormone receptors

• Signal molecules

For all HuSH products, 3-8 constructs are provided as a

panel for each target gene. The multiplicity of the vec-

tors is to ensure optimized inhibited gene expression.

As the shRNA constructs are only sequence verified, it

is strongly recommended that users verify the func-

tions of the shRNA expression constructs in transient

experiments and select the appropriate constructs to

use either in transient studies or in generating stable

cell lines for long-term gene silencing.

While it is possible that stable cell lines can be

generated via plasmid transfection followed by

puromycin drug selection, it is highly recommended

that a retrovirual packaging cell line is used to generate

retroviruses containing the shRNA hairpin expression

cassette and to infect the target cells. It is much more

likely that the shRNA will be expressed if retroviruses

are used in generating the stable cell lines.

Application of HuSH products is illustrated in Fig. 2.

HuSH products can be searched on OriGene’s

website http://www.origene.com/rna/ based on the

properties of the target genes. The search criteria are:

• Nucleotide Sequence, Protein Sequence


• Keywords

• Gene Family

Fig. 2

Cat# GeneID/LocusID description price

TR200002 90 HuSH ACVR1 shRNA Constructs against activin A receptor, type I 380

TR200003 91 HuSH ACVR1B shRNA Constructs against activin A receptor, type IB 380

TR200004 157 HuSH ADRBK2 shRNA Constructs against adrenergic, beta, receptor kinase 2 ) 380

TR200005 207 HuSH AKT1 shRNA Constructs against v-akt murine thymoma viral oncogene homolog 1 380

TR200006 369 HuSH ARAF1 shRNA Constructs against v-raf murine sarcoma 3611 viral oncogene homolog 1 380

TR200007 659 HuSH BMPR2 shRNA Constructs against bone morphogenetic protein receptor, type II (serine/threonine kinase) 380

TR200008 660 HuSH BMX shRNA Constructs against BMX non-receptor tyrosine kinase 380

TR200009 676 HuSH BRDT shRNA Constructs against bromodomain, testis-specific 380

TR200010 695 HuSH BTK shRNA Constructs against Bruton agammaglobulinemia tyrosine kinase 380

TR200011 780 HuSH DDR1 shRNA Constructs against discoidin domain receptor family, member 1 380

TR200012 815 HuSH CAMK2A shRNA Constructs against calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha 380

TR200013 818 HuSH CAMK2G shRNA Constructs against calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma 380

TR200014 984 HuSH CDC2L1 shRNA Constructs against cell division cycle 2-like 1 (PITSLRE proteins) 380

TR200015 1017 HuSH CDK2 shRNA Constructs against cyclin-dependent kinase 2 380




TR200019 1022 HuSH CDK7 shRNA Constructs against cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk- 380

activating kinase)

TR200020 1111 HuSH CHEK1 shRNA Constructs against CHK1 checkpoint homolog (S. pombe) 380

TR200021 1195 HuSH CLK1 shRNA Constructs against CDC-like kinase 1 380


TR200023 1326 HuSH MAP3K8 shRNA Constructs against mitogen-activated protein kinase kinase kinase 8 380

TR200024 1436 HuSH CSF1R shRNA Constructs against colony stimulating factor 1 receptor, formerly McDonough feline 380

sarcoma viral (v-fms) oncogene homolog

TR200025 1452 HuSH CSNK1A1 shRNA Constructs against casein kinase 1, alpha 1 380

TR200026 1453 HuSH CSNK1D shRNA Constructs against casein kinase 1, delta 380

TR200027 1455 HuSH CSNK1G2 shRNA Constructs against casein kinase 1, gamma 2 380

TR200028 1456 HuSH CSNK1G3 shRNA Constructs against casein kinase 1, gamma 3 380

TR200029 1457 HuSH CSNK2A1 shRNA Constructs against casein kinase 2, alpha 1 polypeptide 380

TR200030 1760 HuSH DMPK shRNA Constructs against dystrophia myotonica-protein kinase 380

TR200031 2041 HuSH EPHA1 shRNA Constructs against erythropoietin-producing hepatoma A1 380




TR200035 2050 HuSH EPHB4 shRNA Constructs against erythropoietin-producing hepatoma B4 380

TR200036 2051 HuSH EPHB6 shRNA Constructs against erythropoietin-producing hepatoma B6 380

TR200037 2064 HuSH ERBB2 shRNA Constructs against v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, 380

neuro/glioblastoma derived oncogene homolog (avian)

TR200038 2065 HuSH ERBB3 shRNA Constructs against v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) 380

TR200039 2185 HuSH PTK2B shRNA Constructs against PTK2B protein tyrosine kinase 2 beta 380

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HuSH™—Premade shRNA expression panels

Product offering is subject to change. Refer to www.origene.com for the most updated information.

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TR200040 2242 HuSH FES shRNA Constructs against feline sarcoma oncogene 380

TR200041 2260 HuSH FGFR1 shRNA Constructs against fibroblast growth factor receptor 1 (fms-related tyrosine kinase 2, 380

Pfeiffer syndrome)

TR200042 2264 HuSH FGFR4 shRNA Constructs against fibroblast growth factor receptor 4 380

TR200043 2268 HuSH FGR shRNA Constructs against Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog 380

TR200044 2324 HuSH FLT4 shRNA Constructs against fms-related tyrosine kinase 4 380

TR200045 2475 HuSH FRAP1 shRNA Constructs against FK506 binding protein 12-rapamycin associated protein 1 380

TR200046 2534 HuSH FYN shRNA Constructs against FYN oncogene related to SRC, FGR, YES 380

TR200047 2869 HuSH GPRK5 shRNA Constructs against G protein-coupled receptor kinase 5 380

TR200048 2872 HuSH MKNK2 shRNA Constructs against MAP kinase interacting serine/threonine kinase 2 380

TR200049 2932 HuSH GSK3B shRNA Constructs against glycogen synthase kinase 3 beta 380

TR200050 3055 HuSH HCK shRNA Constructs against hemopoietic cell kinase 380

TR200051 3551 HuSH IKBKB shRNA Constructs against inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta 380

TR200052 3611 HuSH ILK shRNA Constructs against integrin-linked kinase 380

TR200053 3656 HuSH IRAK2 shRNA Constructs against interleukin-1 receptor-associated kinase 2 380

TR200054 3702 HuSH ITK shRNA Constructs against IL2-inducible T-cell kinase 380

TR200055 3716 HuSH JAK1 shRNA Constructs against Janus kinase 1 (a protein tyrosine kinase) 380

TR200056 3791 HuSH KDR shRNA Constructs against kinase insert domain receptor (a type III receptor tyrosine kinase) (KDR), 380

VEGF receptor

TR200057 3815 HuSH KIT shRNA Constructs against v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog 380

TR200058 3932 HuSH LCK shRNA Constructs against lymphocyte-specific protein tyrosine kinase 380

TR200059 3984 HuSH LIMK1 shRNA Constructs against LIM domain kinase 1 380

TR200060 3985 HuSH LIMK2 shRNA Constructs against LIM domain kinase 2 380

TR200061 4117 HuSH MAK shRNA Constructs against male germ cell-associated kinase 380

TR200062 4140 HuSH MARK3 shRNA Constructs against MAP/microtubule affinity-regulating kinase 3 380

TR200063 4638 HuSH MYLK shRNA Constructs against myosin, light polypeptide kinase 380

TR200064 4750 HuSH NEK1 shRNA Constructs against NIMA (never in mitosis gene a)-related kinase 1 380


TR200066 4882 HuSH NPR2 shRNA Constructs against natriuretic peptide receptor B/guanylate cyclase B (atrionatriuretic peptide 380

receptor B)

TR200067 4916 HuSH NTRK3 shRNA Constructs against neurotrophic tyrosine kinase, receptor, type 3 380

TR200068 4921 HuSH DDR2 shRNA Constructs against discoidin domain receptor family, member 2 380

TR200069 5058 HuSH PAK1 shRNA Constructs against p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) 380

TR200070 5063 HuSH PAK3 shRNA Constructs against p21 (CDKN1A)-activated kinase 3 380

TR200071 5127 HuSH PCTK1 shRNA Constructs against PCTAIRE protein kinase 1 380

TR200072 5164 HuSH PDK2 shRNA Constructs against pyruvate dehydrogenase kinase, isoenzyme 2 380

TR200073 5218 HuSH PFTK1 shRNA Constructs against PFTAIRE protein kinase 1 380

TR200074 5347 HuSH PLK shRNA Constructs against polo-like kinase 1 (Drosophila) 380

TR200075 5562 HuSH PRKAA1 shRNA Constructs against protein kinase, AMP-activated, alpha 1 catalytic subunit 380

TR200076 5567 HuSH PRKACB shRNA Constructs against protein kinase, cAMP-dependent, catalytic, beta 380

TR200077 5568 HuSH PRKACG shRNA Constructs against protein kinase, cAMP-dependent, catalytic, gamma 380

TR200078 5579 HuSH PRKCB1 shRNA Constructs against protein kinase C, beta 1 380

TR200079 5581 HuSH PRKCE shRNA Constructs against protein kinase C, epsilon 380

TR200080 5582 HuSH PRKCG shRNA Constructs against protein kinase C, gamma 380

TR200081 5583 HuSH PRKCH shRNA Constructs against protein kinase C, eta 380

TR200082 5588 HuSH PRKCQ shRNA Constructs against protein kinase C, theta 380

TR200083 5590 HuSH PRKCZ shRNA Constructs against protein kinase C, zeta 380

TR200084 5597 HuSH MAPK6 shRNA Constructs against mitogen-activated protein kinase 6 380

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TR200088 5607 HuSH MAP2K5 shRNA Constructs against mitogen-activated protein kinase kinase 5 380

TR200089 5610 HuSH PRKR shRNA Constructs against protein kinase, interferon-inducible double stranded RNA dependent 380

TR200090 5753 HuSH PTK6 shRNA Constructs against PTK6 protein tyrosine kinase 6 380


TR200092 5891 HuSH RAGE shRNA Constructs against renal tumor antigen 380

TR200093 5894 HuSH RAF1 shRNA Constructs against v-raf-1 murine leukemia viral oncogene homolog 1 380

TR200094 6041 HuSH RNASEL shRNA Constructs against ribonuclease L (2’,5’-oligoisoadenylate synthetase-dependent) 380

TR200095 6046 HuSH BRD2 shRNA Constructs against bromodomain containing 2 380

TR200096 6197 HuSH RPS6KA3 shRNA Constructs against ribosomal protein S6 kinase, 90kDa, polypeptide 3 380

TR200097 6198 HuSH RPS6KB1 shRNA Constructs against ribosomal protein S6 kinase, 70kDa, polypeptide 1 380

TR200098 6199 HuSH RPS6KB2 shRNA Constructs against ribosomal protein S6 kinase, 70kDa, polypeptide 2 380

TR200099 6446 HuSH SGK shRNA Constructs against serum/glucocorticoid regulated kinase 380

TR200100 6732 HuSH SRPK1 shRNA Constructs against SFRS protein kinase 1 380

TR200101 6733 HuSH SRPK2 shRNA Constructs against SFRS protein kinase 2 380


TR200103 6789 HuSH STK4 shRNA Constructs against serine/threonine kinase 4 380


TR200105 6792 HuSH CDKL5 shRNA Constructs against cyclin-dependent kinase-like 5 380

TR200106 6795 HuSH AURKC shRNA Constructs against aurora kinase C 380

TR200107 6850 HuSH SYK shRNA Constructs against spleen tyrosine kinase 380


TR200109 7010 HuSH TEK shRNA Constructs against TEK tyrosine kinase, endothelial (venous malformations, multiple 380

cutaneous and mucosal)

TR200110 7272 HuSH TTK shRNA Constructs against TTK protein kinase 380

TR200111 7294 HuSH TXK shRNA Constructs against TXK tyrosine kinase 380

TR200112 7297 HuSH TYK2 shRNA Constructs against tyrosine kinase 2 380

TR200113 7443 HuSH VRK1 shRNA Constructs against vaccinia related kinase 1 380

TR200114 7867 HuSH MAPKAPK3 shRNA Constructs against mitogen-activated protein kinase-activated protein kinase 3 380

TR200115 8444 HuSH DYRK3 shRNA Constructs against dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 380

TR200116 8476 HuSH CDC42BPA shRNA Constructs against CDC42 binding protein kinase alpha (DMPK-like) 380

TR200117 8550 HuSH MAPKAPK5 shRNA Constructs against mitogen-activated protein kinase-activated protein kinase 5 380

TR200118 8558 HuSH CDK10 shRNA Constructs against cyclin-dependent kinase (CDC2-like) 10 380

TR200119 8569 HuSH MKNK1 shRNA Constructs against MAP kinase interacting serine/threonine kinase 1 380

TR200120 8573 HuSH CASK shRNA Constructs against calcium/calmodulin-dependent serine protein kinase (MAGUK family) 380

TR200121 8767 HuSH RIPK2 shRNA Constructs against receptor-interacting serine-threonine kinase 2 380

TR200122 8780 HuSH RIOK3 shRNA Constructs against RIO kinase 3 (yeast) 380


TR200124 8899 HuSH PRPF4B shRNA Constructs against PRP4 pre-mRNA processing factor 4 homolog B (yeast) 380



TR200127 9641 HuSH IKBKE shRNA Constructs against inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon 380

TR200128 9833 HuSH MELK shRNA Constructs against maternal embryonic leucine zipper kinase 380

TR200129 10110 HuSH SGK2 shRNA Constructs against serum/glucocorticoid regulated kinase 2 380

TR200130 10295 HuSH BCKDK shRNA Constructs against branched chain alpha-ketoacid dehydrogenase kinase 380

53ph: 1.888.267.4436 fax: 1.301.340.9254


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TR200131 10420 HuSH TESK2 shRNA Constructs against testis-specific kinase 2 380

TR200132 10461 HuSH MERTK shRNA Constructs against c-mer proto-oncogene tyrosine kinase 380

TR200133 10494 HuSH STK25 shRNA Constructs against serine/threonine kinase 25 (STE20 homolog, yeast) 380

TR200134 10645 HuSH CAMKK2 shRNA Constructs against calcium/calmodulin-dependent protein kinase kinase 2, beta 380

TR200135 10922 HuSH FASTK shRNA Constructs against FAST kinase 380

TR200136 11011 HuSH TLK2 shRNA Constructs against tousled-like kinase 2 380

TR200137 11040 HuSH PIM2 shRNA Constructs against pim-2 oncogene 380

TR200138 11156 HuSH PTP4A3 shRNA Constructs against protein tyrosine phosphatase type IVA, member 3 380

TR200139 11200 HuSH CHEK2 shRNA Constructs against CHK2 checkpoint homolog (S. pombe) 380


TR200141 11344 HuSH PTK9L shRNA Constructs against PTK9L protein tyrosine kinase 9-like (A6-related protein) 380

TR200142 23031 HuSH KIAA0561 shRNA Constructs against PREDICTED: microtubule associated serine/threonine kinase 3 380

TR200143 23178 HuSH PASK shRNA Constructs against PAS domain containing serine/threonine kinase (PASK) 380

TR200144 23227 HuSH KIAA0303 shRNA Constructs against Predicted kinase: KIAA0303 protein (KIAA0303) 380

TR200145 23476 HuSH BRD4 shRNA Constructs against bromodomain containing 4 (BRD4) 380

TR200146 23552 HuSH CCRK shRNA Constructs against cell cycle related kinase (CCRK) 380

TR200147 25778 HuSH KIAA0472 shRNA Constructs against receptor interacting protein kinase 5 (RIPK5) 380

TR200148 25865 HuSH PRKD2 shRNA Constructs against protein kinase D2 380

TR200149 26353 HuSH H11 shRNA Constructs against heat shock 27kDa protein 8 (HSPB8) 380

TR200150 28951 HuSH TRB2 shRNA Constructs against tribbles homolog 2 (Drosophila) 380

TR200151 29941 HuSH pknbeta shRNA Constructs against protein kinase N3 (PKN3) 380

TR200152 29959 HuSH NRBP shRNA Constructs against nuclear receptor binding protein 380

TR200153 30849 HuSH PIK3R4 shRNA Constructs against phosphoinositide-3-kinase, regulatory subunit 4, p150 380

TR200154 51231 HuSH VRK3 shRNA Constructs against vaccinia related kinase 3 380

TR200155 51347 HuSH JIK shRNA Constructs against STE20-like kinase 380

TR200156 51765 HuSH MST4 shRNA Constructs against Mst3 and SOK1-related kinase 380

TR200157 54101 HuSH ANKRD3 shRNA Constructs against receptor-interacting serine-threonine kinase 4 380

TR200158 54861 HuSH SNRK shRNA Constructs against SNF-1 related kinase 380

TR200159 55351 HuSH HSA250839 shRNA Constructs against serine/threonine kinase 32B 380

TR200160 55359 HuSH DKFZp761P1010 shRNA Constructs against protein kinase STYK1 380

TR200161 55781 HuSH FLJ10074 shRNA Constructs against RIO kinase 2 (yeast) 380

TR200162 55872 HuSH RIOK2 shRNA Constructs against T-LAK cell-originated protein kinase 380

TR200163 56924 HuSH PAK6 shRNA Constructs against p21(CDKN1A)-activated kinase 6 380

TR200164 56997 HuSH CABC1 shRNA Constructs against chaperone, ABC1 activity of bc1 complex like (S. pombe) 380

TR200165 57143 HuSH ADCK1 shRNA Constructs against aarF domain containing kinase 1 380

TR200166 57144 HuSH PAK7 shRNA Constructs against p21(CDKN1A)-activated kinase 7 380

TR200167 57147 HuSH PACE-1 shRNA Constructs against ezrin-binding partner PACE-1 380

TR200168 57172 HuSH CAMK1G shRNA Constructs against calcium/calmodulin-dependent protein kinase IG 380


TR200170 57410 HuSH SCYL1 shRNA Constructs against SCY1-like 1 (S. cerevisiae) 380

TR200171 57551 HuSH KIAA1361 shRNA Constructs against serine/threonine protein kinase TAO1 homolog 380


TR200173 79834 HuSH KIAA2002 shRNA Constructs against Predicted kinase: KIAA2002 protein 380

TR200174 79858 HuSH NEK11 shRNA Constructs against NIMA (never in mitosis gene a)- related kinase 11 380


TR200176 80216 HuSH LAK shRNA Constructs against lymphocyte alpha-kinase 380

TR200177 81788 HuSH SNARK shRNA Constructs against likely ortholog of rat SNF1/AMP-activated protein kinase 380

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TR200178 83694 HuSH RPS6KL1 shRNA Constructs against ribosomal protein S6 kinase-like 1 380

TR200179 83732 HuSH RIOK1 shRNA Constructs against RIO kinase 1 (yeast) 380

TR200180 84930 HuSH FLJ14813 shRNA Constructs against microtubule associated serine/threonine kinase-like 380

TR200181 85366 HuSH MYLK2 shRNA Constructs against myosin light chain kinase 2, skeletal muscle 380

TR200182 91461 HuSH LOC91461 shRNA Constructs against Predicted kinase: LOC91461 380

TR200183 92335 HuSH LYK5 shRNA Constructs against protein kinase LYK5 (LYK5) 380

TR200184 115701 HuSH HAK shRNA Constructs against heart alpha-kinase (HAK) 380

TR200185 130399 HuSH ACVR1C shRNA Constructs against activin A receptor, type IC 380

TR200186 140469 HuSH MYO3B shRNA Constructs against myosin IIIB (MYO3B) 380

TR200187 167359 HuSH MGC42105 shRNA Constructs against Predicted kinase: MGC42105 380

TR200188 197259 HuSH FLJ34389 shRNA Constructs against Predicted kinase: FLJ34389 380


TR200190 203447 HuSH NRK shRNA Constructs against Nik related kinase 380

TR200191 282974 HuSH STK32C shRNA Constructs against serine/threonine kinase 32C 380

TR200192 284086 HuSH NEK8 shRNA Constructs against NIMA (never in mitosis gene a)- related kinase 8 380

TR200193 340371 HuSH LOC340371 shRNA Constructs against Predicted kinase: LOC340371 380

TR200194 190 HuSH NR0B1 shRNA Constructs against nuclear receptor subfamily 0, group B, member 1 380

TR200195 238 HuSH ALK shRNA Constructs against anaplastic lymphoma kinase 380

TR200196 1789 HuSH DNMT3B shRNA Constructs against DNA (cytosine-5-)-methyltransferase 380

TR200197 1956 HuSH EGFR shRNA Constructs against epidermal growth factor receptor 380

TR200198 2100 HuSH ESR2 shRNA Constructs against estrogen receptor 2 380

TR200199 2103 HuSH ESRRB shRNA Constructs against estrogen-related receptor beta 380

TR200200 3164 HuSH NR4A1 shRNA Constructs against nuclear receptor subfamily 4, group A, member 1 380

TR200201 4929 HuSH NR4A2 shRNA Constructs against nuclear receptor subfamily 4, group A, member 2 380

TR200202 5469 HuSH PPARBP shRNA Constructs against PPAR binding protein 380


TR200204 6096 HuSH RORB shRNA Constructs against RAR-related orphan receptor B 380

TR200205 7157 HuSH TP53 shRNA Constructs against tumor protein p53 (Li-Fraumeni syndrome) 380

TR200206 7182 HuSH NR2C2 shRNA Constructs against nuclear receptor subfamily 2, group C, member 2 380

TR200207 7376 HuSH NR1H2 shRNA Constructs against nuclear receptor subfamily 1, group H, member 2 380

TR200208 8856 HuSH NR1I2 shRNA Constructs against nuclear receptor subfamily 1, group I, member 2 380

TR200209 9572 HuSH NR1D1 shRNA Constructs against nuclear receptor subfamily 1, group D, member 1 380

TR200210 9886 HuSH RHOBTB1 shRNA Constructs against Rho-related BTB domain containing 380


TR200212 29904 HuSH EEF2K shRNA Constructs against elongation factor-2 kinase 380

TR200213 23 HuSH ABCF1 shRNA Constructs against ATP-binding cassette, sub-family F (GCN20), member 1 380

TR200214 2066 HuSH ERBB4 shRNA Constructs against v-erb-a erythroblastic leukemia viral oncogene homolog 4 380


TR200216 4486 HuSH MST1R shRNA Constructs against macrophage stimulating 1 receptor 380

TR200217 5465 HuSH PPARA shRNA Constructs against peroxisome proliferative activated receptor, alpha 380

TR200218 5663 HuSH PSEN1 shRNA Constructs against presenilin 1 (Alzheimer disease 3) 380

TR200219 5728 HuSH PTEN shRNA Constructs against phosphatase and tensin homolog (mutated in multiple advanced cancers 1) 380

TR200220 5915 HuSH RARB shRNA Constructs against retinoic acid receptor, beta 380

TR200221 5919 HuSH RARRES2 shRNA Constructs against retinoic acid receptor responder 2 380

TR200222 5920 HuSH RARRES3 shRNA Constructs against retinoic acid receptor responder 3 380

TR200223 6258 HuSH RXRG shRNA Constructs against retinoid X receptor, gamma 380

TR200224 7098 HuSH TLR3 shRNA Constructs against toll-like receptor 3 380

55ph: 1.888.267.4436 fax: 1.301.340.9254


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TR200225 9252 HuSH RPS6KA5 shRNA Constructs against ribosomal protein S6 kinase, 90kDa 380

TR200226 9325 HuSH TRIP4 shRNA Constructs against thyroid hormone receptor interactor 4 380


TR200228 63925 HuSH ZNF335 shRNA Constructs against zinc finger protein 335 380

TR200229 102 HuSH ADAM10 shRNA Constructs against a disintegrin and metalloproteinase domain 10 380


TR200231 1969 HuSH EPHA2 shRNA Constructs against EphA2 380

TR200232 3091 HuSH HIF1A shRNA Constructs against hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix 380

transcription factor)

TR200233 3172 HuSH HNF4A shRNA Constructs against hepatocyte nuclear factor 4, alpha 380

TR200234 4292 HuSH MLH1 shRNA Constructs against mutL homolog 1 colon cancer 380


TR200236 5468 HuSH PPARG shRNA Constructs against peroxisome proliferative activated receptor, gamma 380

TR200237 7097 HuSH LRTLR2 shRNA Constructs against toll-like receptor 2 380

TR200238 8202 HuSH NCOA3 shRNA Constructs against nuclear receptor coactivator 3 380

TR200239 8648 HuSH NCOA1 shRNA Constructs against nuclear receptor coactivator 1 380

TR200241 9975 HuSH NR1D2 shRNA Constructs against nuclear receptor subfamily 1, group D, member 2 380

TR200242 79705 HuSH LRRK1 shRNA Constructs against leucine-rich repeat kinase 1 380

TR200243 2261 HuSH FGFR3 shRNA Constructs against fibroblast growth factor receptor 3 380

TR200244 4593 HuSH MUSK shRNA Constructs against muscle, skeletal, receptor tyrosine kinase 380

TR200245 5916 HuSH RARG shRNA Constructs against retinoic acid receptor, gamma 380

TR200246 7006 HuSH TEC shRNA Constructs against tec protein tyrosine kinase 380

TR200247 7067 HuSH THRA shRNA Constructs against thyroid hormone receptor, alpha 380

TR200248 7068 HuSH THRB shRNA Constructs against thyroid hormone receptor, beta 380

TR200249 7099 HuSH TLR4 shRNA Constructs against toll-like receptor 4 380



TR200252 51135 HuSH IRAK4 shRNA Constructs against interleukin-1 receptor-associated kinase 4 380

TR200253 149420 HuSH LOC149420 shRNA Constructs against casein kinase 380

TR200254 156 HuSH ADRBK1 Constructs against adrenergic, beta, receptor kinase 1 380

TR200255 208 HuSH AKT2 Constructs against v-akt murine thymoma viral oncogene homolog 2 380

TR200256 351 HuSH APP Constructs against amyloid beta (A4) precursor protein (protease nexin-II, Alzheimer disease) 380

TR200257 367 HuSH AR Constructs against androgen receptor (dihydrotestosterone receptor; testicular feminization; 380

spinal and bulbar muscular atrophy; Kennedy disea

TR200258 816 HuSH CAMK2B Constructs against calcium/calmodulin-dependent protein kinase (CaM kinase) II beta 380

TR200259 896 HuSH CCND3 Constructs against cyclin D3 380

TR200260 898 HuSH CCNE1 Constructs against cyclin E1 380

TR200261 1025 HuSH CDK9 Constructs against cyclin-dependent kinase 9 (CDC2-related kinase) 380

TR200262 1030 HuSH CDKN2B Constructs against cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) 380

TR200263 1032 HuSH CDKN2D Constructs against cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) 380

TR200264 1263 HuSH PLK3 Constructs against polo-like kinase 3 (Drosophila) 380

TR200265 1454 HuSH CSNK1E Constructs against casein kinase 1, epsilon 380

TR200266 1611 HuSH DAP Constructs against death-associated protein 380

TR200267 1612 HuSH DAPK1 Constructs against death-associated protein kinase 1 380


TR200269 1725 HuSH DHPS Constructs against deoxyhypusine synthase 380

TR200270 1869 HuSH E2F1 Constructs against E2F transcription factor 1 380

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TR200271 2002 HuSH ELK1 Constructs against ELK1, member of ETS oncogene family 380

TR200272 2011 HuSH MARK2 Constructs against MAP/microtubule affinity-regulating kinase 2 380

TR200273 2045 HuSH EPHA7 Constructs against EPH receptor A7 380

TR200274 2046 HuSH EPHA8 Constructs against EPH receptor A8 380

TR200275 2047 HuSH EPHB1 Constructs against EPH receptor B1 380

TR200276 2049 HuSH EPHB3 Constructs against EPH receptor B3 380

TR200277 2057 HuSH EPOR Constructs against erythropoietin receptor 380

TR200278 2099 HuSH ESR1 Constructs against estrogen receptor 1 380

TR200279 2101 HuSH ESRRA Constructs against estrogen-related receptor alpha 380

TR200280 2114 HuSH ETS2 Constructs against v-ets erythroblastosis virus E26 oncogene homolog 2 (avian) 380

TR200281 2263 HuSH FGFR2 Constructs against fibroblast growth factor receptor 2 (bacteria-expressed kinase, keratinocyte 380

growth factor receptor, craniofacial dysost

TR200282 2308 HuSH FOXO1A Constructs against forkhead box O1A (rhabdomyosarcoma) 380

TR200283 2580 HuSH GAK Constructs against cyclin G associated kinase 380

TR200284 2868 HuSH GRK4 Constructs against G protein-coupled receptor kinase 4 380

TR200285 2870 HuSH GRK6 Constructs against G protein-coupled receptor kinase 6 380

TR200286 2891 HuSH GRIA2 Constructs against glutamate receptor, ionotropic, AMPA 2 380

TR200287 2931 HuSH GSK3A Constructs against glycogen synthase kinase 3 alpha 380

TR200288 3315 HuSH HSPB1 Constructs against heat shock 27kDa protein 1 380

TR200289 3480 HuSH IGF1R Constructs against insulin-like growth factor 1 receptor 380

TR200290 3645 HuSH INSRR Constructs against insulin receptor-related receptor 380

TR200291 3654 HuSH IRAK1 Constructs against interleukin-1 receptor-associated kinase 1 380

TR200292 3725 HuSH JUN Constructs against v-jun sarcoma virus 17 oncogene homolog (avian) 380

TR200293 3726 HuSH JUNB Constructs against jun B proto-oncogene 380

TR200294 4041 HuSH LRP5 Constructs against low density lipoprotein receptor-related protein 5 380

TR200295 4086 HuSH SMAD1 Constructs against SMAD, mothers against DPP homolog 1 (Drosophila) 380

TR200296 4092 HuSH SMAD7 Constructs against SMAD, mothers against DPP homolog 7 (Drosophila) 380

TR200297 4094 HuSH MAF Constructs against v-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian) 380

TR200298 4139 HuSH MARK1 Constructs against MAP/microtubule affinity-regulating kinase 1 380

TR200299 4217 HuSH MAP3K5 Constructs against mitogen-activated protein kinase kinase kinase 5 380

TR200300 4296 HuSH MAP3K11 Constructs against mitogen-activated protein kinase kinase kinase 11 380

TR200301 4790 HuSH NFKB1 Constructs against nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (p105) 380

TR200302 4793 HuSH NFKBIB Constructs against nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta 380

TR200303 4846 HuSH NOS3 Constructs against nitric oxide synthase 3 (endothelial cell) 380

TR200304 4915 HuSH NTRK2 Constructs against neurotrophic tyrosine kinase, receptor, type 2 380

TR200305 4919 HuSH ROR1 Constructs against receptor tyrosine kinase-like orphan receptor 1 380

TR200306 5128 HuSH PCTK2 Constructs against PCTAIRE protein kinase 2 380

TR200307 5170 HuSH PDPK1 Constructs against 3-phosphoinositide dependent protein kinase-1 380

TR200308 5184 HuSH PEPD Constructs against peptidase D 380

TR200309 5241 HuSH PGR Constructs against progesterone receptor 380

TR200310 5467 HuSH PPARD Constructs against peroxisome proliferative activated receptor, delta 380

TR200311 5585 HuSH PKN1 Constructs against protein kinase N1 380

TR200312 5586 HuSH PKN2 Constructs against protein kinase N2 380

TR200313 5587 HuSH PRKD1 Constructs against protein kinase D1 380

TR200314 5604 HuSH MAP2K1 Constructs against mitogen-activated protein kinase kinase 1 380



57ph: 1.888.267.4436 fax: 1.301.340.9254


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TR200317 5918 HuSH RARRES1 Constructs against retinoic acid receptor responder (tazarotene induced) 1 380

TR200318 5925 HuSH RB1 Constructs against retinoblastoma 1 (including osteosarcoma) 380

TR200319 5979 HuSH RET Constructs against ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1,

Hirschsprung disease) 380

TR200320 6095 HuSH RORA Constructs against RAR-related orphan receptor A 380

TR200321 6195 HuSH RPS6KA1 Constructs against ribosomal protein S6 kinase, 90kDa, polypeptide 1 380

TR200322 6256 HuSH RXRA Constructs against retinoid X receptor, alpha 380

TR200323 6257 HuSH RXRB Constructs against retinoid X receptor, beta 380

TR200324 6259 HuSH RYK Constructs against RYK receptor-like tyrosine kinase 380

TR200325 6300 HuSH MAPK12 Constructs against mitogen-activated protein kinase 12 380

TR200326 6714 HuSH SRC Constructs against v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) 380

TR200327 6722 HuSH SRF Constructs against serum response factor (c-fos serum response element-binding transcription factor) 380

TR200328 6776 HuSH STAT5A Constructs against signal transducer and activator of transcription 5A 380

TR200329 6777 HuSH STAT5B Constructs against signal transducer and activator of transcription 5B 380

TR200330 6794 HuSH STK11 Constructs against serine/threonine kinase 11 (Peutz-Jeghers syndrome) 380

TR200331 7026 HuSH NR2F2 Constructs against nuclear receptor subfamily 2, group F, member 2 380

TR200332 7046 HuSH TGFBR1 Constructs against transforming growth factor, beta receptor I (activin A receptor type II-like 380

kinase, 53kDa)

TR200333 7075 HuSH TIE Constructs against tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 380

TR200334 7101 HuSH NR2E1 Constructs against nuclear receptor subfamily 2, group E, member 1 380

TR200335 7128 HuSH TNFAIP3 Constructs against tumor necrosis factor, alpha-induced protein 3 380

TR200336 7301 HuSH TYRO3 Constructs against TYRO3 protein tyrosine kinase 380

TR200337 7421 HuSH VDR Constructs against vitamin D (1,25- dihydroxyvitamin D3) receptor 380

TR200338 7465 HuSH WEE1 Constructs against WEE1 homolog (S. pombe) 380

TR200339 7535 HuSH ZAP70 Constructs against zeta-chain (TCR) associated protein kinase 70kDa 380

TR200340 7918 HuSH BAT4 Constructs against HLA-B associated transcript 4 380

TR200341 8536 HuSH CAMK1 Constructs against calcium/calmodulin-dependent protein kinase I 380

TR200342 8711 HuSH TNK1 Constructs against tyrosine kinase, non-receptor, 1 380

TR200343 8717 HuSH TRADD Constructs against TNFRSF1A-associated via death domain 380

TR200344 8772 HuSH FADD Constructs against Fas (TNFRSF6)-associated via death domain 380

TR200345 8792 HuSH TNFRSF11A Constructs against tumor necrosis factor receptor superfamily, member 11a, activator of NFKB 380

TR200346 8986 HuSH RPS6KA4 Constructs against ribosomal protein S6 kinase, 90kDa, polypeptide 4 380

TR200347 9088 HuSH PKMYT1 Constructs against membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase 380

TR200348 9113 HuSH LATS1 Constructs against LATS, large tumor suppressor, homolog 1 (Drosophila) 380

TR200349 9261 HuSH MAPKAPK2 Constructs against mitogen-activated protein kinase-activated protein kinase 2 380

TR200350 9328 HuSH GTF3C5 Constructs against general transcription factor IIIC, polypeptide 5, 63kDa 380

TR200351 9344 HuSH TAOK2 Constructs against TAO kinase 2 380

TR200352 9578 HuSH CDC42BPB Constructs against CDC42 binding protein kinase beta (DMPK-like) 380

TR200353 10155 HuSH TRIM28 Constructs against tripartite motif-containing 28 380

TR200354 10188 HuSH ACK1 Constructs against activated Cdc42-associated kinase 1 380

TR200355 10221 HuSH TRIB1 Constructs against tribbles homolog 1 (Drosophila) 380

TR200356 10392 HuSH CARD4 Constructs against caspase recruitment domain family, member 4 380

TR200357 10783 HuSH NEK6 Constructs against NIMA (never in mitosis gene a)-related kinase 6 380

TR200358 22983 HuSH MAST1 Constructs against microtubule associated serine/threonine kinase 1 380

TR200359 23043 HuSH TNIK Constructs against TRAF2 and NCK interacting kinase 380

TR200360 23235 HuSH SIK2 Constructs against salt-inducible serine/threonine kinase 2 380


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TR200362 26524 HuSH LATS2 Constructs against LATS, large tumor suppressor, homolog 2 (Drosophila) 380

TR200363 27102 HuSH HRI Constructs against heme-regulated initiation factor 2-alpha kinase 380

TR200364 51755 HuSH CRK7 Constructs against CDC2-related protein kinase 380

TR200365 51776 HuSH ZAK Constructs against sterile alpha motif and leucine zipper containing kinase AZK 380

TR200366 55072 HuSH RNF31 Constructs against ring finger protein 31 380

TR200367 55589 HuSH BMP2K Constructs against BMP2 inducible kinase 380

TR200368 55739 HuSH FLJ10769 Constructs against hypothetical protein FLJ10769 380

TR200369 65018 HuSH PINK1 Constructs against PTEN induced putative kinase 1 380

TR200370 79012 HuSH MGC8407 Constructs against hypothetical protein MGC8407 380

TR200371 85443 HuSH KIAA1765 Constructs against KIAA1765 protein 380

TR200372 112858 HuSH TP53RK Constructs against TP53 regulating kinase 380

TR200373 114783 HuSH LMTK3 Constructs against lemur tyrosine kinase 3 380

TR200374 9284 HuSH NPIP Constructs against NM_006985 - Locus_ID 9284 380

59ph: 1.888.267.4436 fax: 1.301.340.9254


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Product Listing Cat. No. PricepRS retroviral gene silencing vector TR20003 150

pRS-GFP-v1 (shRNA) plasmid TR20001 150

pRS-Luc-v1 (shRNA) plasmid TR20002 150

pRS vector

Introduction:

Other than the premade shRNA plasmids, OriGene

also provides the blank pRS vector for researchers to

clone in their own shDNA sequences.

The vector system has been validated for retrovirus

production, puromycin selection, stable cell line

production, shRNA expression and gene silencing.

Oligos against target genes are designed and the

oligo pairs are annealed and inserted into the BamHI

– BbsI sites of the pRS plasmid. Due to significant

error rates associated with oligo synthesis, sequence

verification of the shRNA insert is highly re-

commended. OriGene only sells sequence verified

shRNA plasmids.

Due to the biological complexity of the RNAi, only

about 25-30% of the shRNA vectors are expected to

reduce the target gene expression by >70%. The first

step using shRNA vectors is to validate the

functionality of the vectors in transiently transfected

cells. We recommend to co-transfect cells, i.e.,

HEK293, with the shRNA vector and the target gene

to determine the effect of the shRNA vectors against

the target gene and to select only the ones with

inhibitory function.

Once a specific shRNA vector(s) is selected, stable cell

lines can be established by transfecting the plasmid

into a retroviral packaging cell and by using the virus

containing supernatant to infect the target cells.

Puromycin is added to the infected cells for selecting

stable cell lines expressing the functional shRNA.

Applications

• Cloning of shRNA oligos

• Inhibiting the expression of the target genes

• Generating stable cell lines

shRNA oligos

pBS Ori

pRS5522 bp

U6 promoter

Ampicillin

SV40 early promoter

Puro

3‘ LTR5‘ LTR

BamH I

Not I

Hind IIIBbs I

Sal I

Cla I

Eco R I

Fig. 1

pRS plasmid map. The shRNA oligos will be cloned between

BamHI and BbsI, immediately downstream of U6 promoter. A

puromycin selection marker is present. Both 5’ and 3’ LTRs are

from MMLV and used to generate retroviruses in appropriate

packaging cells.

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pRS-GFP-v1 (shRNA) plasmid

Product Description

Enhanced green fluorescent protein (EGFP) shRNA

expression plasmid. Transfection grade purified

mammalian expression plasmid carrying GFP se-

quence. When transfected into mammalian cells, it

expresses a short-haripin RNA (shRNA) from the U6

promoter, resulting in the inhibition of the target

gene. Significant inhibition of the GFP expression

can be detected using both fluorescence microscopy

and western blot analysis with GFP specific antibody

(Fig. 2).

Applications: Positive control for the inhibition of

EGFP expression

Product content: 10 ug of pRS-luc-v1 and 10 ug of

pRS-NegCon-v1

Fig. 2

shRNA plasmid pRS-GFP-v1 plasmid. HEK293 cells were

transfected with plasmids carrying eGFP (A), eGFP and shRNA-GFP-

v1 at 1:100 ratio (B), eGFP and NegCont-v1 (C) using FuGene 6.

Three days after transfection, the cells were analyzed on a

fluorescence microscope and on Western blot with a GFP specific

antibody.

pRS-Luc-v1 (shRNA) plasmid

Product Description

Firefly luciferase (Luc) shRNA expression plasmid.

Transfection grade purified mammalian expression

plasmid carrying luciferase sequence. When

transfected into mammalian cells, it expresses a

short-haripin RNA (shRNA) from the U6 promoter,

resulting in the inhibition of the target gene. In HEK

cells co-transfected with a luciferase plasmid and

pRS-luc-v1, approximately 90% inhibition of the

luciferase activity can be observed (Fig. 3).

Applications: Positive control for the inhibition of

luciferase expression

Product content: 10 ug of pRS-luc-v1 and 10 ug of

pRS-NegCon-v1

shRNA plasmid pRS-Luc-v1 plasmid. HEK293 cells were

transfected with plasmids carrying Luciferase (Pos. Cont.), Luc and

shRNA-Luc-v1 at 1:100 ratio, eGFP and NegCont-v1 using FuGene 6.

Three days after transfection, the cells were analyzed with Britelite

(PE) on Victor 3 plate reader (PE).

Fig. 3

62 www.origene.com

Full-Length cDNA cloningRapid-Screen™ arrayed cDNA libraries

Rapid-Screen™ Longest-CloneSM services

Sure-RACE™ 5’-RACE Kit

Yeast two-hybrid expression cDNA libraries

Full-

len

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libra

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64 www.origene.com

Product Listing Cat. No. PriceHuman (Master plates)

Human Brain (adult) LAB-1001 925

Human Brain (fetal) LLFB-1001 925

Human Colon LCO-1001 925

Human Heart LHT-1001 925

Human Kidney LKD-1001 925

Human Liver LLI-1001 925

Human Lung LLU-1001 925

Human Peripheral blood leukocytes LPBL-1001 925

Human Placenta LLPC-1001 925

Human Skeletal muscle LMU-1001 925

Human Small intestine LSI-1001 925

Human Spleen LLSP-1001 925

Human Testis LTS-1001 925

Sub-Plates

All sub-plates for the above libraries [Sub-plates are designated by the library’s 100prefix followed by the number (column) and letter (row) of the positive well(s) detected on the master plate, e.g. “RAB-5A”]

Rapid Screen™ arrayed cDNA libraries

The complete thirteen human and/or six mouse cDNA library panels are available for licensing to investigators

who are interested in simultaneous multi-tissue screens to:

1) increase the chances of identifying “full-length” cDNA clones that are present in more than one tissue, or

2) isolate cDNA clones of genes whose major sites of expression have not been fully described.

Full-

len

gth

cDN

Acl

on

ing

Rapi

dSc

reen

™ar

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Product Listing Cat. No. PriceMouse (Master plates)

Mouse Brain (adult) MAB-1001 925

Mouse Embryo (12.5-day) MEB-1001 925

Mouse Embryo (19-day) MEA-1001 925

Mouse Liver MLI-1001 925

Mouse Testis MTS-1001 925

Mouse Thymus MTM-1001 925

Rat (Master plates)

Rat Brain (adult) RAB-1001 925

Sub-Plates

All sub-plates for the above libraries [Sub-plates are designated by the library’s 100prefix followed by the number (column) and letter (row) of the positive well(s) detected on the master plate, e.g. “RAB-5A”]

Introduction:

Gene identification is increasing at a rapid pace, due

in large part to technologies such as expressed

sequence tag (EST) discovery, as well as the wealth

of sequence information being produced by the

Genome Sequencing Projects. Once a gene is

identified, the next step is to clone the full-length

cDNA in order to perform sequence and expression

analyses, and ultimately to discover its function in

vivo.

Although it has been suggested that most of the

human genes have already been tagged, full-length

cDNA clones have been obtained for less than 50

percent of them. There are several reasons for this

lag. Until now, obtaining a full-length clone has been

very labor-intensive, time consuming and expensive.

More importantly, the technology for constructing

full-length libraries from which clones are isolated

has lagged behind the technology for tagging the

genes in the first place. For example, most libraries

to date have a relatively low average insert size, due

to the fact that it has been difficult to reverse

transcribe mRNAs with extensive secondary

structures and that smaller-sized cDNAs are much

more easily ligated into a vector than larger cDNAs.

The Rapid-Screen™ Arrayed cDNA Library Panels*

were designed as a fast, inexpensive, and easy way to

clone full-length cDNAs. The library quality was

improved by first isolating double-stranded cDNAs of

different size-fractions and then ligating them se-

parately into the vector. The cloning process has been

made faster and more economical by having the

libraries arrayed in 96-well plates and developing a

screening procedure that is PCR based. Since the

screening is PCR-based, vector and gene-specific

primers can be used in concert to determine the size of

the various cDNA inserts after the very first PCR screen,

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thus eliminating the need to isolate many clones and

then find out which, if any, are full-length. A screening

service is also offered, in which OriGene will simul-

taneously screen all twelve human or all six mouse

Rapid-Screen™ cDNA libraries and provide the longest

clone of any gene of interest found in the libraries.

*U.S. Patent #6,316,193


How does Rapid-Screen™ work?

The Rapid-Screen™ cDNA Library Panels were

designed for EST to “full-length” gene cloning and

require just three sets of PCRs to identify the desired

cDNA clone. The first PCR analysis is performed in a

96-well “Master Plate,” where each well contains

plasmid DNA from 5,000 clones. Having identified the

positive well(s) by gel electrophoresis of the reaction

products, the second PCR analysis is carried out in the

corresponding 96-well “Sub-Plate(s),” which contains

all 5,000 clones from the positive well of the Master

Plate arrayed at only 50 clones per well. Since this

plate contains glycerol stocks of E. coli instead of

plasmid DNA, the cells can be diluted and plated out

once a positive well(s) has been identified. Analysis of

96 single colonies by PCR leads to the identification of

the positive clone from the Sub-Plate well.

To ensure that a clone detected in a specific well in the

Master Plate will be represented in the appropriate

Sub-Plate, the Sub-Plates were not derived by dilution

of individual wells from the Master Plate (a process

which may lose specific clones). Instead, the Sub-

Plates were generated first and the wells from each

Sub-Plate were then pooled to generate the Master

Plate. To guarantee that clones from different wells in

the Master Plate were each independently-derived, the

Sub-Plates were generated with primary trans-

formants that had not been amplified.

ADVANTAGES

Isolate large cDNA clones with only three 96-well PCRs

Libraries designed tocontain large inserts

Can identify longest cloneeven before its isolation

Final clone is not aPCR product

No messy radioactive probes or labor-intensive

colony lifts

“Full-length” clones canbe immediately transfected

into mammalian cells

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What was done to obtain large inserts?

A great deal of foresight has been incorporated into

the development of these arrayed cDNA panels, so

that they are user-friendly and cost-effective. Since

they are intended for researchers searching for

longer than average-sized cDNA clones, double-

stranded cDNAs (synthesized from poly A+ RNA

using oligo (dT) primers) were size-fractionated in a

low-melt agarose gel and the various size-fractions

were independently ligated to the vector to minimize

competition for insertion by smaller cDNAs. The

insert sizes of random clones from the various

ligation reactions were then determined (Fig. 1). This

allowed the Rapid-Screen™ libraries to be spiked

with more clones from the large-sized fraction,

thereby giving them a much larger average insert

size (Table 1). In addition to helping find full-length

clones, a large average insert size allows for

Fig. 1

14

12

10

8

6

4

2

0

Num

ber

ofco

loni

es

1.5 - 2.5 kb fraction2.5 - 5 kb fraction> 5kb fraction

0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 6kb

*Data for this table were obtained by randomly picking 48 colonies per library, preparing plasmid DNA from each, and analyzing the insertsby restriction enzyme digestion followed by gel electrophoresis.

Brain (adult)Brain (fetal)ColonHeartKidneyLiverLungPeripheral blood leukocytesPlacentaSkeletal muscleSmall intestineSpleenTestis

96989394889591829284919882

0.9-6.00.9-10.00.8-6.51.0-9.00.8-3.70.8-3.21.0-6.01.0-5.01.0-9.00.4-6.50.5-6.00.5-10.00.6-5.5

2.42.82.92.91.82.03.22.43.62.52.62.42.5

Brain (adult)Embryo (12.5-day)Embryo (19-day)LiverTestisThymus

919499949789

0.6-10.02.0-7.00.5-6.51.0-6.51.2-8.00.6-8.0

3.23.53.53.53.53.2

Human

Mouse

Brain (adult) 900.9-5.02.6Rat

LIBRARY % INSERTSSIZE RANGEAVERAGE SIZE (kb)

Table 1*

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screening of fewer members due to the general

finding that longer transcripts tend to be less

abundant in the cell. Since the cDNAs were

directionally-cloned into the CMV expression vector

pCMV6-XL4 (Fig. 2), a vector-derived 5’ PCR primer

can be used in conjunction with a gene-specific 3’

reverse primer to identify the longest cDNA clone of

interest (see Fig. 4).

Can the clones be expressed inmammalian cells?

Once a full-length clone has been isolated and

sequenced, it can be directly transfected into

mammalian cells for expression studies. The library

plasmid, pCMV6-XL4, contains the SV40 origin of

replication for propagation in mammalian cells.

Moreover, the plasmid contains the CMV promoter,

which will drive expression of the insert if it contains

a translational start site. Finally, the human growth

hormone polyadenylation signal is present to effect

proper transcriptional termination.

PRODUCT COMPONENTS

One 96-well Master Plate(enough material for

5 screens)

5’-vector primer

Four sheets of aluminum foil sealing tape Fig. 2

ColElOri

SV40 Ori

f1 Ori

Ampicillin

hGH poly A

Sac I

T7 promoterM13 reverse

Sma I

MCS

CMVpromoter

pCMV6-XL4

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1 Perform 96-well PCR on the Master

Plate (5,000 clones/well) using 2 gene-

specific primers; run samples in an

agarose gel to identify positive well(s);

order corresponding Sub-Plate.

2 Perform 96-well PCR

on the Sub-Plate (50 clones/well)

using same primers as for Master

Plate; run samples in an agarose gel

to identify positive well(s); dilute and

plate positive well on an LB amp plate

to obtain single colonies.

3 Perform PCRs on 96 individual colonies

using same primers as above; run

samples in an agarose gel to identify

positive colony; grow cells and prepare

miniprep DNA; analyze plasmid DNA

by restriction enzyme digestion and

DNA sequencing.

Product Use

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Product Application

Is a 96-well PCR necessary?

If it is not possible or desirable to perform 96

simultaneous PCRs, then pools of DNA from the

wells in the Master Plate can be made. Using 5 µL of

DNA from each well of the Master Plate, twelve pools

(40,000 clones/pool) of DNA can be made from

columns 1 through 12, and/or eight pools (60,000

clones/pool) of DNA can be made from rows A

through H. These 20 pools can be analyzed by PCR

using two gene-specific primers. In the example

shown in Fig. 3, there is a single clone in this library

and it is located in well E9 of the Master Plate. The

one caveat to this approach is that since there are

40,000-60,000 independent clones per pool, rather

than only 5,000 from an individual well, both gene-

specific primers must be highly specific. The pooling

technique is most informative when working with a

low-abundance versus a high-abundance transcript.

Can the size of the longest clone bedetermined without first isolating it?

If multiple positive clones are detected, one may

wish to determine their insert sizes before

proceeding to their isolation. In the example shown

in Fig. 4, PCR analysis using a gene-specific primer-

pair (panel A) revealed positive clones in pools 1, 4

and 9 (40,000 clones/pool). Since the inserts were

directionally cloned into the library vector,

simultaneous analysis using the 5’ vector primer and

the 3’ gene-specific primer (panel B) allowed

determination of the extent of upstream sequences

(5.5 kb in two of the clones and 3.5 kb in the other).

Similarly, use of the 5’ gene-specific primer with the

3’ vector primer (panel C) allowed determination of

the extent of downstream sequences (1.5 kb in all

three clones). In this example, one could determine

the insert sizes to be 6.5 kb in two clones and 4.5 kb

in the other, even before proceeding to their

isolation. The 6.5-kb clones turned out to be full-

length.

ABCDEFGH

1 2 3 4 5 6 7 8 9 10 11 12

Fig. 3

A B C D E F G H

1 2 3 4 5 6 7 8 9 10 11 12

A

kb

— 0.5

B

Pool

1

Pool

4

Pool

9

C

— 5.5— 3.5

— 1.5

1 2 3 4 5 6 7 8 9 10 11 12

Fig. 4

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What factors are important when “long” PCR is used with Rapid-Screen™?

When the 5’ vector primer is used in conjunction with a

3’ gene-specific primer to identify long clones, the

choice of the appropriate Taq DNA polymerase is

important. Fig. 5 illustrates the use of three different

enzymes (labeled 1, 2 and 3), with either a 3-min (top) or

a 7-min (bottom) extension time, on four selected

Master Plate wells (5,000 clones/well), each containing

a single positive clone with insert sizes ranging from 2.5

to 8.0 kb in length. Enzyme 1 was able to amplify all of

the fragments using only a 3-min extension time, so the

7-min extension time experiment was not necessary.

Enzyme 2 was not able to amplify any of the fragments

with a 3-min extension time, but it could successfully

amplify the 2.5- and 3.0-kb fragments when a 7-min

extension time was used. Enzyme 3 partially amplified

two of the fragments when a 3-min extension time was

used, but it could successfully amplify all four

fragments with a 7-min extension time. These

experiments demonstrate the importance of choosing

an efficient Taq DNA polymerase. If only small

fragments are being amplified, then any of the three

enzymes tested are suitable. To amplify longer

fragments, a more efficient Taq DNA polymerase and/or

a longer extension time is required.

Can Rapid-Screen™ be used to clone different forms of an alternatively-spliced transcript?

Rapid-Screen™ panels can be used to clone

alternatively-spliced transcripts, even if the desired

transcript is less abundant than its parent transcript.

The detection of different-sized fragments by using

the 5’ vector primer plus the 3’ gene-specific primer

is sometimes attributable to alternative splicing

rather than to truncated transcripts (if the alternative

splicing has already been characterized, then gene-

specific primers that differentiate between the two

spliced forms can be designed). As shown in Fig. 6, a

pair of gene-specific primers was used on a Master

Plate now to detect two alternatively-spliced

transcripts of a particular gene. In this instance, the

longer transcript was more abundant than the

shorter form; the former appeared in three wells

(5,000 clones/pool) and the shorter form in two wells.

MM M

Enz 1 Enz 2 Enz 3

Fig. 6

Fig. 5

1 2 3 4 5 6 7 8 9 10 11 12

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Product Description

What is the Screening Service?

OriGene offers customized screening of either a 12-

tissue human or a 6-tissue mouse Rapid-Screen™

Arrayed cDNA Library Panel* (Super-Plate), and

subsequent isolation of the “longest clone” from the

selected library. Since most genes are expressed in

several tissues, the screening of a multi-tissue panel,

containing a total of either 6-million human or 3-

million mouse size-selected clones, increases the

probability of isolating a full-length clone. For

example, even though a particular transcript may be

known to be abundant in a certain tissue, differences

in starting RNA quality or in cDNA yield for

individual libraries may result in distortions in

representation. In other words, the library with the

most representations of a particular gene does not

assure the presence of a full-length clone.

How is the screen performed?

Initially, PCR will be performed using gene-specific

primers, either provided by the customer or

designed by OriGene, to determine whether clones

can be identified in any of the libraries. If no clones

are found, the customer will not be charged and the

service will be terminated. However, if the initial

screening is successful, then the relative sizes of the

cDNA clones will be determined and the longest

clone from the libraries will be isolated. Hence, there

is no need to provide multiple clones. Restriction

enzyme and PCR analyses will be performed on

purified DNA prepared from the clone to confirm

both its integrity and the size of the cDNA insert. An

aliquot of the purified DNA, a bacterial stock of the

clone, and a photograph of an ethidium bromide-

stained agarose gel detailing the analyses will be

provided to the customer.

What are the twelve human librariescurrently available?

Brain (adult), brain (fetal), heart, kidney, liver, lung,

muscle, peripheral blood leukocytes (PBL), placenta,

small intestine, spleen, and testis.

What are the six mouse libraries currently available?

Brain (adult), embryo (12.5-day), embryo (19-day),

liver, testis, and thymus.

*U.S. Patent #6,316,193

Product Listing Cat. No. Price

Human Library Screening Service HRSS-001 3900

Mouse Library Screening Service MRSS-001 3900

Primer Design and Synthesis RSPD-001 300

Rapid-Screen™ Longest-CloneSM services

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What materials need to be provided forthis service?

1. Set of exonic PCR primers. One forward (5’) and

two contiguous reverse (3’) gene specific primers are

required. The distance between the positions of the

forward and reverse primers within the cDNA should

ideally be about 500 base-pairs. This information is

required prior to the screening. OriGene can design

and synthesize specific primers for an additional

charge.

2. Positive control DNA. A cDNA clone, upon which

the PCR primer sequences were based, is required

and will be used as a positive control.

3. Information on the target gene (optional). This

includes (i) expected size of target mRNA based on

Northern blotting, (ii) expected sites of expression of

target mRNA, and (iii) sequence of EST or

incomplete cDNA used to generate the PCR primers

(only if deemed appropriate).

How is the initial screen performed?

The first step in the Rapid-Screen™ Longest-CloneSM

Screening Service involves a Super-Plate screen, in

which each of the eight rows from all twelve of the

human or all six of the mouse panels have been

combined into pools (60,000 clones/pool, eight

pools/library). If no clones are detected in the Super-

Plate screen, there is no charge for the screen.

However, if positives are detected, which is usually

the case, then the well containing the longest clone

will be determined using the 5’ vector-specific primer

in combination with the 3’ gene-specific primer (see

Fig. 4 of the Rapid-Screen™ Arrayed cDNA Library

Panels section). The longest clone will then be

isolated from the library in which it is found. Fig. 1

shows the results of a typical human Super-Plate

screen. In this example, the cDNA of interest was

absent in fetal brain, liver, and spleen. It was

detected in low abundance in adult brain, heart,

kidney, muscle, PBL, and testis. The cDNA was found

in high abundance in lung, placenta, and small

intestine.

Brain (adult)

Brain (fetal)

Heart

Kidney

Liver

Lung

Muscle

PBL

1 2 3 4 5 6 7 8 1 2 3 4

Placenta

Small

Intestine

Spleen

Testis

Fig. 1

1 2 3 41 2 3 4 5 6 7 8

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What characterizations are performed onthe isolated clone?

Once the longest cDNA clone has been isolated, its

identity will be confirmed by PCR analysis. The size

of the cDNA insert will be determined by restriction

enzyme mapping and by PCR, using vector-specific

primers and purified plasmid DNA. Fig. 2 shows such

an analysis by agarose gel electrophoresis for an 8-kb

clone that was obtained by the Screening Service.

Purified plasmid DNA of the clone was digested by

Not I, which cuts on either side of the insert (lane 1).

The upper band is the insert while the lower band is

the vector. PCR was performed using two gene-

specific primers to confirm the identity of the clone

(lane 2). The length of the 3’ end of the clone was

determined by PCR using a 5’ gene-specific primer

and a 3’ vector-specific primer (lane 3). The length of

the 5’ end of the clone was determined by PCR using

a 5’ vector-specific primer and a 3’ gene-specific

primer (lane 4).

Further Information

Before ordering, it is recommended that customers

first contact OriGene for further details about the

Rapid-Screen™ Longest-CloneSM Screening Service.

Please call toll-free at 888-267-4436 (U.S. only) or at

301-340-3188, or contact us by e-mail at

[email protected].

Fig. 2

— 2

— 1

— 8

kb

— 4

M 1 2 3 4 M

— 0.4

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Introduction

Traditional cDNA libraries frequently yield clones

which are incomplete and where sequences

representing the mRNA 5’ ends are missing. The

RACE technology, a PCR-based approach for rapid

amplification of cDNA ends, has been particularly

useful for completing the missing portions of cDNA

clones and yielding full-length sequences of

expressed regions of genes.

To expand the utilities of the RACE technology,

OriGene has now developed a PCR-ready RACE

panel which will allow simultaneous 5’ end analysis

of transcripts from 24 individual human tissues or 24

mouse tissues and developmental stages. The Sure-

RACE™ panels contain double-stranded cDNAs that

have been arrayed in multi-well plates. Sure-RACE™

is designed with the recognition that an increasing

number of genes have been identified that utilize

alternate RNA start-sites resulting from the use of

tissue-specific or developmental stage-specific

transcriptional promoters. As well, an increasing

number of genes have also been found to utilize

alternate 5’ exons, sometimes resulting in protein

products with different N-terminal amino acid

sequences.

Product Listing Cat. No. PriceHuman Sure-RACE™ Panel HRAA-101 620(4 complete panels)

Mouse Sure-RACE™ Panel MRAB-101 495(4 complete panels)

Sure-RACE™ 5’-RACE kit

ADVANTAGES

Broad spectrum of tissues:scan 24 individual human or

mouse tissues

High sensitivity: detect rare transcripts

Simultaneous analysis ofalternatively-spliced

transcripts and alternateRNA start-sites

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Product Description

Development of the Sure-RACE™ Panels began with

the selection of high-quality mRNAs. The integrity of

each of the poly A+ RNA was determined by Northern

blot analysis using ß-actin cDNA as the hybridization

probe. Only mRNAs that fulfilled stringent criteria of

intactness were used for cDNA synthesis.

It is well-established that mRNAs have complex

secondary structures which may act as strong stops

during first-strand cDNA synthesis using standard

reverse transcriptase (RT). As a consequence, the

resulting single-stranded cDNAs may not be

representative of the starting mRNAs in that their 5’

ends may not be reverse transcribed. OriGene has

successfully used a dual-cycling procedure to

overcome this problem. Specifically, the initial cDNA

synthesis was performed using standard procedures

with MMLV RT at 42°C, followed by a second round of

synthesis with thermostable Tth DNA polymerase at

75°C. Cycling of high-processivity/low-thermostability

enzyme and low-processivity/high-thermostability

enzyme, which facilitates read-through of RNA

secondary structures, was repeated a second time to

ensure the efficiency of the reaction.

Conventional double-stranded cDNA synthesis

involves the “blunting” of the cDNAs, which results

in the loss of 5’ end sequences of 20-200 nucleotides.

To avoid sequence loss, the first-strand cDNAs were

“tailed” at their 3’ ends with dCTP (see Fig. 1). The

terminal transferase reaction was optimized to

produce a tail of 12-15 dC residues. Second-strand

cDNA synthesis was initiated by the annealing of an

adaptor-oligo(dG) primer to the oligo(dC) tail at the 3’

ends of the first-strand cDNA molecules. Escherichia

coli DNA polymerase I was used for second-strand

synthesis to reduce the nucleotide-incorporation

error rate. Additionally, RNase H was added to nick

the mRNA and to allow internal priming. Finally, T4

DNA ligase was added to facilitate joining of

fragments primed by oligo(dG) and nicked mRNA.

The Sure-RACE™ panels were carefully assembled

from 24 major human tissues or 24 major mouse

tissues and different developmental stages. The

RACE-ready cDNAs are provided in two

3’CCCCCCCCCCC

5’ADP1..ADP2..GGGGGGGGGGG

5’

5’

3’

3’CCCCCCCCCCC

3’CCCCCCCCCCC

5’ADP1..ADP2..GGGGGGGGGGGH

Dual cycling synthesis

dCTP tailing

Second strand synthesis

fig.SR-1

VTTTTTTTTTTTTTTCTCGAG 5’

AAAAAAAAAAAAAA 3’

AAAAAAAAAAAAAA 3’

TTTTTTTTTTTTTTCTCGAG 5’


AAAAAAAAAAAAAA 3’

AAAAAAAAAAAAAAGAGCTC 3’


AAAAAAAAAAAAAA 3’

Fig. 1

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concentrations (5x and 1x), to accommodate the

analysis of both rare and abundant transcripts. They

are arrayed in 48-well (6 x 8 wells) PCR plates. Four

panels are provided per order to allow replication of

the findings and assurance of the specificity of the

observed RACE products.

Product Use

By simply performing a nested PCR analysis, using

the two contiguous adaptor-specific primers

provided with the kit and two contiguous gene-

specific primers designed by the investigator, the

Sure-RACE™ panels will: [1] markedly increase the

probability of completing the 5’ ends of transcripts

which are rare or have complex secondary

structures; [2] allow the identification of alternate

RNA start-sites either within the same tissue, in

different tissues or at different developmental

stages; and [3] provide evidence for alternate RNA

splicings at the 5’ end, such as the use of alternate

first exons or an identical first exon but alternate

downstream exons, which may result in proteins

with nonidentical amino acid sequences. Any or all of

this information is derived from direct sequencing of

the PCR products generated by the human or mouse

Sure-RACE™ panels.

Sure-RACE™ is performed using two rounds of PCR.

The first round uses Adaptor Primer 1 and a gene-

specific primer to enrich for the specific cDNAs, and

the second round uses Adaptor Primer 2 and a

nested gene-specific primer to further amplify the

specific target(s). About 20 PCR cycles are routinely

performed in the first round, followed by 30-35

cycles in the second round (nested) PCR. The

specificity of the gene specific primers is a critical

factor.

PRODUCT COMPONENTS

Four identical 48-well PCRplates containing two

dilutions of 24 human or24 mouse RACE-ready cDNAs

Adaptor primers for PCR

Control primers fortransferrin receptor

Free sample of DNAQuanti-Ladder™

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5 Document the results by imaging/

photography and elute bands of interest

for DNA sequence analysis or cloning.

1 Prepare a 48-well PCR master mix including

Adaptor Primer 1 and gene-specific primer 1;

aliquot the solution into each well of the

Sure-RACE™ panel.

2 Place the plate into a

thermal cycler and

proceed with

primary PCR.

3 Dilute primary PCR and

perform secondary PCR in

second 48-well plate using

Adaptor Primer 2 and

gene-specific primer 2.

4 Upon completion of the secondary PCR,

analyze the reaction products by agarose

gel electrophoresis.

Product Use

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Product Application

A. Human Sure-RACE™ Panel

The Human Sure-RACE™ panel was assembled from

24 major tissues. The RACE-ready cDNAs are

provided in two concentrations (5x and 1x) to

accommodate the analysis of both rare and

abundant transcripts. They are arrayed in a 48-well (6

x 8 wells) PCR plate in the order indicated in Fig. 2.

The Human Sure-RACE™ panel was used to analyze

the 5’ end sequences of transcripts from the highly-

expressed transferrin receptor gene (Fig. 3). The

results show a single major component of the

appropriate size in virtually every tissue, as well as

minor components of heterogeneous sizes which

were derived from cDNAs with truncated 5’ ends.

When the template concentration was reduced to

one-fifth, the latter components became less

detectable.

The Sure-RACE™ panel can be used to complete the

5’-end sequences of long and rare transcripts with

extensive secondary structure (Fig. 4). The ataxia

telangiectasia (ATM) gene encodes an ubiquitous 12-

kb transcript with extensive conformations and

somewhat heterogeneous 5’ ends.

B. Mouse Sure-RACE™ Panel

The Mouse Sure-RACE™ panel was assembled from

24 major tissues and developmental stages. The

adult tissues were prepared from outbred Swiss

Webster mice and the breast tissues from outbred

CD1 mice. The RACE-ready cDNAs are provided in

two concentrations (5x and 1x) to accommodate the

analysis of both rare and abundant transcripts. They

are arrayed in a 48-well (6 x 8 wells) PCR plate in the

order indicated in Fig. 5.

Examples of applications of the Mouse Sure-RACE™

panel are illustrated here. The Sure-RACE™ panel

can be used to analyze the 5’ end sequences of

transcripts. Figure 6 shows an analysis if the 5’ end

of the interleukin 1 receptor antagonist mRNA. The

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BrainHeartKidneySpleenLiverColon

LungSmall IntestineMuscleStomachTestisPlacenta

Prostate GlandPBLFetal BrainFetal LiverFatMammary Gland

Fig. 2

Fig. 3

Fig. 4

1 2 3 4 5 6 7 8 9 10 11 12

13 14 15 16 17 18 19 20 21 22 23 24

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1.2.3.4.5.6.

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7.8.9.

10.11.12.

19.20.21.22.23.24.

BrainHeartKidneySpleenThymusLiver

Adrenal GlandSalivary GlandUterusProstate GlandEmbryo 8-9 dayEmbryo 9.5 day

StomachSmall IntestineMuscleLungTestisSkin

Embryo 12.5 dayEmbryo 19 dayBreast/VirginBreast/PregnantBreast/LactatingBreast/Involuting

5x

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results show a single major band of the appropriate

size in most tissues.

The Sure-RACE™ panel can also be used to identify

differentially-spliced transcripts that contain

alternate 5’ exons that are utilized in different tissues

(Fig. 7). The fibroblast growth factor 1 (FGF-1) gene

makes use of different 5’ exons to regulate its

expression.

Fig. 7

Fig. 6

1 2 3 4 5 6 7 8 9 10 11 12

13 14 15 16 17 18 19 20 21 22 23 24

1 2 3 4 5 6 7 8 9 10 11 12

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Yeast Strains

EGY48 Contains 6 LexA binding sites upstream of LEU2 reporter gene; most sensitive

EGY194 Contains 4 LexA binding sites upstream of LEU2 reporter gene; less sensitive

EGY188 Contains 2 LexA binding sites upstream of LEU2 reporter gene; least sensitive

EGY40 Contains 0 LexA binding sites upstream of LEU2 reporter gene; control strain

RFY206 Used for specificity testing in a mating assay

E. coli Strains

KC8 trp- strain for recovery of plasmids

Expression Plasmids

pEG202 For constitutive expression of LexA-bait fusion protein (Fig. 2 on Page 84)

pEG202-NLS For constitutive expression of LexA-NLS-bait fusion protein

pJG4-5 Galactose-inducible expression of NLS-B42-HA tag-target protein (Fig. 2 on Page 84)

pJG4-6 Galactose-inducible expression of HA tag-target protein

pNLexA Used for fusing LexA to the C-terminus rather than the N-terminus of the bait

LacZ Plasmids

pSH18-34 Contains 8 LexA binding sites upstream of LacZ reporter gene; most sensitive

pJK103 Contains 2 LexA binding sites upstream of LacZ reporter gene; less sensitive

pRB1840 Contains 1 LexA binding site upstream of the LacZ reporter gene; least sensitive

pJK101 Used for testing the ability of the LexA fusion protein to enter the nucleus

Product Listing Cat. No. PriceDupLEX-A™ yeast two-hybrid system DKT-100 1800includes all items below

DupLEX-A™ yeast two-hybrid system

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Control Plasmids

pSH17-4 Positive control; activates reporter genes on its own

pRHFM1 Negative control; does not interact with positive target

pLexA-Max Negative control; does not interact with positive target

pBAIT LexA-bait fusion protein which interacts with pTARGET fusion protein

pTARGET B42-HA tag-target fusion protein which interacts with pBAIT fusion protein

Primers

5’ Bait Primer Primer for sequencing vector-insert junction in pEG202

5’ Target Primer Primer for PCR and sequencing in pJG4-5

3’ Target Primer Primer for PCR and sequencing in pJG4-5

82 www.origene.com

Introduction

One objective of studying a particular biochemical

pathway or process is to determine all of the

molecular components involved and define how they

interact with one another.The increasing pace of gene

discovery is providing researchers with a multitude of

genes whose functions have yet to be determined.

Based upon their translated sequences, individual

gene products may be assigned to specific classes of

proteins such as kinases or phosphatases, but this

type of analysis does not provide information on what

specific role they play in the cell. However, knowing

what protein(s) they interact with may provide insight

into which biochemical pathway they belong, and

hence what their function(s) may be.

Pioneered by Fields and Song in 19891 and Gyuris et

al. in 19932, the yeast two-hybrid system is a method

for detecting protein-protein interactions in vivo. The

yeast two-hybrid system relies on the finding that

some transcriptional activator proteins have

separable DNA binding and transcriptional activation

domains. Neither domain can activate transcription

on its own and must be tethered to on another to

activate transcription. Interaction between two

proteins can be tested by fusing one with the DNA

binding domain and the other with the

transcriptional activation domain of the activator

protein. If the two proteins interact, then the

transcriptional activator is reconstituted and can

bind to its binding site and activate transcription of a

reporter gene. Yeast is used since it is eukaryotic, can

be genetically modified easily, and can be

transformed. The yeast Gal4 protein and the E. coli

LexA protein are two transcriptional activators that

have been used to develop such yeast two-hybrid

systems.

The yeast two-hybrid system can be used to test for

interactions between two known proteins, but its

greatest utility has been in screening cDNA

expression libraries for proteins that will interact

with the specific bait being tested. Libraries have

been constructed from many different organisms,

tissues, and cell lines for screening by the yeast two-

1. A novel genetic system to detect protein-protein interactions.Nature. 1989 Jul 20;340(6230):245-6.

2. Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2.Cell. 1993 Nov 19;75(4):791-803.

hybrid system. The chances of successfully finding

an interacting partner rely heavily on choosing the

right tissue or cell-type for the protein of interest, as

well as on the quality of the library itself. The yeast

two-hybrid system and variations thereof are

becoming increasingly popular tools in gene

discovery and functional studies.

Product Description

The DupLEX-A™ Yeast Two-Hybrid System exploits

the fact that an E. coli transcriptional activation

domain, B42, and an E. coli DNA binding protein,

LexA, can activate transcription of a reporter when

LexA is bound to its DNA recognition sequence and

is also tethered to the B42 activation domain. Neither

the LexA protein nor the B42 activation domain

alone can activate transcription. The two-hybrid

system involves fusing the LexA protein with a bait

protein “X” and the B42 activation domain with a

target protein “Y” and co-expressing them in yeast

containing a reporter gene with LexA binding sites

inserted into its promoter. If “X” and “Y” interact,

then the B42 activation domain is anchored to the

promoter of the reporter gene through the LexA

protein and transcriptional activation of the reporter

can be detected (Fig. 1). In the DupLEX-A™ system,

“X” is the bait and “Y” is a library of proteins.

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ADVANTAGESof the DupLEX-A™ System over other LexA or Gal4-based yeast

two-hybrid systems

Ability to eliminate false positives since target protein expression

is inducible

Ability to screen potentially toxic target proteins

Reporters with varying sensitivities are available so that baits which

activate transcription on their own canpotentially still be assayed by using

a less sensitive reporter

Allows co-immunoprecipitation of interacting proteins using antibodies

to HA tag or LexAp

Easy assays are available to determine whether or not a particular

bait protein can enter the yeast nucleus and bind LexA operators

Libraries are unamplified and are provided in the form of

ready-to-use DNA

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The bait vector pEG202 and the target or library

vector pJG4-5 are shown in Fig. 2.

DupLEX-A™ yeast two-hybrid system libraries

OriGene has an extensive list of cDNA libraries

available for use with the DupLEX-A™ Yeast Two-

Hybrid System kit. For each library, oligo(dT)-primed

cDNA was synthesized and cloned directionally

between the EcoRI and XhoI sites of pJG4-5, the

activation domain expression vector used in the

DupLEX-A™ system. After plating the primary

bacterial transformants, the cells were scraped from

the plates, pooled, an aliquot was removed and

frozen away, and plasmid DNA was purified from the

rest. Approximately 100 µg of purified, ready-to-use

library DNA is provided. Thus the researcher need not

perform the laborious task of library DNA isolation.

Moreover, the provided library DNA has never been

amplified.

A frozen E. coli glycerol stock of the plasmid library is

also provided which can be readily used to prepare

additional DNA, if needed.

The list of libraries includes those made from specific

human, rat, and mouse tissues, as well as from whole

organisms. In addition, a special emphasis has been

made on constructing libraries from several breast

and prostate cancer tissues and cell lines.

It should be noted that these libraries, while designed

to complement the yeast two-hybrid system, may also

be used as standard plasmid libraries for isolation of

cDNA clones using conventional nucleic acid-based

screening strategies.

84 www.origene.com

GAA TTC CCG GGG ATC CGT CGA CCA TGG CGG CCG CTC GAG TCG ACC TGC AGCEco RI Bam HI Sal I Nco l Not l Xho l Sal l

Amp R

pBR322ori

ADH promLexA ADH

term

2 micron

HIS3

TRP1

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2 micron

Amp R

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GAL prom

B42-HA tag

nucl. local. B42 acid blob domain HA tag

ATG EcoRV EcoRI XhoI

Amp R

pEG202(10.2 kb)

pJG4-5(6.4 kb)

Fig. 2

Human

Human Fetal Brain* 3.5 x 106 0.2 – 1.6 kb DLH-101 1250(Frontal Cortex, 22-week)

Human Fetal Kidney 3.0 x 106 0.2 – 2.0 kb DLH-106 1250

Human Liver (Adult) 1.1 x 107 0.3 – 4.8 kb DLH-100 1250

Human Fetal Liver 8.6 x 106 0.4 – 2.5 kb DLH-105 1250

Human Ovary(Adult) 4.0 x 106 0.4 – 6.1 kb DLH-114 1250

Human PBL 1.0 x 107 0.3 – 2.5 kb DLH-104 1250(Peripheral Blood Leukocytes)

HeLa Cell* Line 9.6 x 106 0.3 – 3.3 kb DLH-103 1250

Jurkat T-cell Line 5.7 x 106 0.3 – 4.0 kb DLH-115 1250

MG63 Cell Line 3.0 x 106 0.3 – 2.5 kb DLH-118 1250(Osteosarcoma Cell Line)

WI-38 Cell Line 5.7 x 106 0.4 – 5.0 kb DLH-102 1250(Human Lung Fibroblast)*(Serum-starved)

SKOV3 Cell Line 5.0 x 106 0.2 – 2.3 kb DLH-111 1250(Ovarian Cancer Cell Line)

Mouse

Mouse Brain (Adult) 6.1 x 106 0.3 – 1.7 kb DLM-100 1250

Mouse Embryo (Whole, 19-day) 1.0 x 107 0.2 – 2.5 kb DLM-110 1250

Mouse Liver (Adult) 9.5 x 106 0.3 – 3.2 kb DLM-102 1250

Mouse Ovary (Adult) 4.0 x 106 0.3 – 1.5 kb DLM-103 1250

Mouse Skeletal Muscle (Adult) 7.2 x 106 0.4 – 3.5 kb DLM-111 1250

Mouse Spleen (Adult) 1.0 x 107 0.2 – 2.5 kb DLM-101 1250

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Product ListingRNA Source # of Indt. Insert Size Cat. No. Price

Clones Range

Yeast two-hybrid expression cDNA libraries

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RNA Source # of Indt. Insert Size Cat. No. PriceClones Range

Rat

Rat Adipocyte 1.0 x 107 0.4 – 5.0 kb DLR-103 1250(9 week-old Zucker rat)

Rat Brain (Adult) 4.5 x 106 0.3 – 3.4 kb DLR-102 1250

Rat Testis (Adult) 8.0 x 106 0.5 – 4.0 kb DLR-101 1250

Rat Thymus (Adult) 8.2 x 106 0.3 – 4.5 kb DLR-100 1250

Breast-specific

MCF7 Cell 1.0 x 107 0.6 – 4.5 kb DLH-112 1250(Estrogen-Depleted)

MCF7 Cell (Serum-grown) 1.0 x 107 0.4 – 3.5 kb DLH-117 1250

MCF7 Cell (Estrogen-treated) 1.0 x 107 0.4 – 4.6 kb DLH-113 1250

SKBR3 Cell (Estrogen- 5.4 x 106 0.5 – 4.0 kb DLH-116 1250receptor negative)

Mouse Breast, Normal 1.0 x 107 0.4 – 5.5 kb DLM-107 1250(Virgin)

Mouse Breast, Normal 6.3 x 106 0.4 – 5.3 kb DLM-108 1250(Pregnant, 12-Day)

Mouse Breast, Normal 1.0 x 107 0.4 – 3.1 kb DLM-105 1250(Lactating)

Mouse Breast, Normal 1.0 x 107 0.4 – 7.0 kb DLM-106 1250(Involuting)

Prostate-specific

Human Prostate, Normal 6.4 x 106 0.3 – 1.7 kb DLH-107 1250(Pooled from 8 adults)

Human Prostate, Tumor 4.8 x 106 0.2 – 2.0 kb DLH-108 1250(Pooled from 8 adults)

LNCaP Cell (Untreated) 2.9 x 106 0.3 – 2.0 kb DLH-109 1250

LNCaP Cell (Treated with 30 4.6 x 106 0.3 – 2.3 kb DLH-110 1250nM R1881)

Mouse Prostate, Normal 3.7 x 106 0.3 – 4.0 kb DLM-104 1250

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RNA Source # of Indt. Insert Size Cat. No. PriceClones Range

Other

C. elegans (Adult) 3.8 x 106 0.2 – 3.6 kb DLCE-100 1250

D. melanogaster (Adult) 1.8 x 106 0.3 – 1.8 kb DLDM-100 1250

S. cerevisiae*** (Genomic) 4.0 x 106 0.3 – 4.0 kb DLY-100 1250

MDBK Cell (Bovine Kidney) 5.8 x 106 0.3 – 1.7 kb DLBK-100 1250

*The libraries marked with a single asterisk (*) were amplified an additional time and plasmid DNA was purified from the re-amplified cells.**The average size of the inserts was calculated by restriction digestion analysis of plasmid DNA from 48 randomly-chosen colonies from each library. Please note that smaller insertsare more informative than larger inserts in the yeast two-hybrid system screen. Even so, there are larger inserts present in these libraries.***The S. cerevisiae library is from strain S288C, with inserts cloned into the EcoRI site of pJG4-5.

TRP1

ADHterm

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Amp R

pUC ori

GAL prom

B42-HA tag

nucl. local. B42 acid blob domain HA tag

ATG EcoRV EcoRI XhoI

Amp R

pJG4-5(6.4 kb)

Fig. 1

Introduction

OriGene provides an extensive list of expression

cDNA libraries. As a pivotal component of OriGene’s

DupLEX-A Yeast Two-Hybrid System kit, these

libraries has been utilized extensively by scientists

and were cited in numerous publications1-11. For each

library, oligo(dT)-primerd cDNA was synthesized

from high quality mRNAs and cloned uni-

directionally between EcoRI and XhoI site of pJG4-5

(Fig. 1), the activation domain expression vector

used in the DupLEX-A system. After plating the

primary bacterial transformants, the cells were

scraped from the plates, pooled, an aliquot was

removed and frozen away and the plasmid was

purified from the rest. Thus the researchers need not

perform the laborious task of library DNA isolation.

Moreover, the provided library DNA has never been

amplified.

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In addition, while designed to complement the yeast

two-hybrid system, these libraries can be used as

standard plasmid libraries for isolation of cDNA

clones using conventional nucleic acid-based


The list of libraries includes those made from specific

human, rat, and mouse tissues, as well as from

whole organisms. In addition, a special emphasis

has been made on constructing libraries from

several breast and prostate cancer tissues and cell

lines.


For each library, oligo(dT)-primed cDNA was

synthesized and cloned directionally between the

EcoRI and XhoI sites of pJG4-5. After plating the

primary bacterial transformants, the cells were

scraped from the plates, pooled, an aliquot was

removed and frozen away, and plasmid DNA was

purified from the rest. Approximately 100 µg of

purified, ready-to-use library DNA is provided. Thus

the researcher need not perform the laborious task of

library DNA isolation. Moreover, the provided library

DNA has never been amplified.

A frozen E. coli glycerol stock of the plasmid library is

also provided which can be readily used to prepare

additional DNA, if needed.

It should be noted that these libraries, while designed

to complement the yeast two-hybrid system, can also

be used as standard plasmid libraries for isolation of

cDNA clones using conventional nucleic acid-based


ADVANTAGES

Supplied as ready-to-use library DNA plus bacterial

glycerol stock

Largest selection of cDNA libraries for TRP1-compatible yeast

two-hybrid systems

Several libraries designed specifically for breast and prostate

cancer research

1. Coordinated functions of WSS1, PSY2 and TOF1 in the DNA damage response.Nucleic Acids Res. 2004 Dec 14;32(22):6519-30.2. GIPC recruits GAIP (RGS19) to attenuate dopamine D2 receptor signaling.Mol Biol Cell. 2004 Nov;15(11):4926-37.3.Ribosomal S6 kinase as a mediator of keratinocyte growth factor-induced activation ofAkt in epithelial cells.Mol Biol Cell. 2004 Jul;15(7):3106-13.4. Small interfering RNAs mediate sequence-independent gene suppression and induceimmune activation by signaling through toll-like receptor 3.J Immunol. 2004 Jun 1;172(11):6545-9.5. Murine tenascin-W: a novel mammalian tenascin expressed in kidney and at sites ofbone and smooth muscle development.J Cell Sci. 2004 Feb 1;117(Pt 4):571-81.6. p57Kip2 regulates actin dynamics by binding and translocating LIM-kinase 1 to thenucleus.J Biol Chem. 2003 Dec 26;278(52):52919-23.

7. An interaction between a MYC protein and an EREBP protein is involved intranscriptional regulation of the rice Wx gene.J Biol Chem. 2003 Nov 28;278(48):47803-11.8. PDZ Domain-mediated interaction of interleukin-16 precursor proteins with myosinphosphatase targeting subunits.J Biol Chem. 2003 Oct 24;278(43):42190-9.9. The Lafora disease gene product laforin interacts with HIRIP5, a phylogeneticallyconserved protein containing a NifU-like domain.Hum Mol Genet. 2003 Sep 15;12(18):2359-68.10. Candida albicans Ssa1/2p is the cell envelope binding protein for human salivaryhistatin 5.J Biol Chem. 2003 Aug 1;278(31):28553-61.11. Fas-associated death domain protein interacts with methyl-CpG binding domainprotein 4: a potential link between genome surveillance and apoptosis.Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5211-6.

Gene expressionRapid-Scan™: PCR-based gene expression profiling panel

Real-Time-Scan™: Real-Time PCR-based gene expression profiling panel

Northern Blot

Multiple-Choice™ cDNAs

Blue-Ribbon™ Poly A+ and Total RNAs

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Human Tissue Rapid-Scan Panel (24 Tissues) HSCA-101 675

Human Brain Rapid-Scan Panel (12 Parts) BSCD-101 520

Human Breast Cancer Rapid-Scan Panel TSCE-101 675(24 Normals/Tumors)

Mouse Tissue Rapid-Scan Panel (24 Tissues) MSCB-101 550

Mouse Brain Rapid-Scan Panel (48 Parts/Stages) NSCF-101 675

Drosophila Tissue Rapid-Scan Panel (12 Parts/Stages) DSCC-101 375

Human Tissue Real-Time Rapid-Scan Panel HSCR-101 (<5) 680(48 Tissues) New!

HSCR-101 (5–10) 575

HSCR-101 (>10) 500

Rapid-Scan

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Introduction

Rapid-Scan™ is a PCR-based system, using high-

quality, first-strand cDNAs derived from different

tissues and/or developmental stages, to generate a

"comprehensive" expression profile of any cloned

gene or EST from either human, mouse or Drosophila.

Individual first-strand cDNAs have been tested to

assure that low abundance and long transcripts are

represented, and have been normalized using an

internal standard. The cDNAs were then serially-

diluted over a 4-log range and arrayed in a 48- or 96-

well PCR plate. This ensures that the amplification

reaction will be within the linear range and, hence, will

facilitate semi-quantitative determination of relative

mRNA accumulation in the various tissues and

developmental stages. With this non-radioactive

technique, one can generate a comprehensive

expression profile for any given gene in just three

hours. All that is required is a 48- or 96-well PCR using

gene-specific primers, followed by electrophoretic

analysis of the products in an agarose gel.

The Rapid-Scan Gene Expression Panels are highly

sensitive and provide a quick readout about the sites

of transcript accumulation. If more exact quan-

tification and sizing of transcript are desired, this can

be accommodated by use of Northern RNA Blots.

To take advantage of Real-Time PCR technology and

to provide more accurate profiling of gene

expression, OriGene launched the new Real-Time

Rapid-Scan panel. This panel is based on the same

principle, yet the cDNA template was normalized by

real-time PCR. The advantage of Real-Time panel

over the conventional Rapid-Scan is as follows.

1. Real-time PCR platform compatible

2. Wider tissue spectrum: 48 tissue in Real-time panel

vs. 24 tissues in conventional panel

3. More accurate quantification of gene expression

level

4. Simplified procedure: no more agarose gel

electrophoresis and gel imaging steps.

ADVANTAGES

Fast and non-radioactive

Simultaneous examination ofmany different tissues and/or

developmental stages

Highly sensitive andsemi-quantitative

Simultaneous analysis ofalternatively-spliced transcripts

Real-time PCR compatibility with newly-launched 48-tissue

human panel

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Rapid-Scan™ Gene Expression Panels were

assembled by selecting either 24 frequently-studied

human tissues, 12 human brain parts, 24 human

breast cancer (normal/tumor) samples, 24 major

mouse tissues, 48 mouse brain parts and stages, or

12 Drosophila tissues and stages. To avoid detection

of individual differences in gene expression, tissues

were pooled, whenever possible, from multiple

individuals.

Total RNA was isolated and subjected to oligo (dT)

selection. The recovered poly A+ RNA was then

examined by Northern blot hybridization to confirm

RNA integrity. The poly A+ RNA was then used to

synthesize first-strand cDNA, using oligo (dT)

primers and MMLV reverse transcriptase. Individual

cDNAs were confirmed to be free of genomic DNA

contamination and to contain complete reverse

transcripts of selected rare and long mRNAs.

The first-strand cDNAs from each tissue were then

subjected to normalization, such that they all contain

an equivalent concentration of a control reverse

transcript. Each cDNA was diluted in water to a series

of four concentrations (labeled 1000x, 100x, 10x and

1x), with the lowest concentration (1x) being

approximately 1 pg. The diluted cDNAs were

subsequently arrayed in a 48- or 96-well PCR plate.

Six conventional Rapid-Scan™ Gene Expression

Panels are currently available: 24 human tissues

(normalized against actin), 12 human brain parts

(normalized against actin), 24 human breast cancer

(normal/tumor) samples (normalized against actin),

24 major mouse tissues (normalized against actin),

48 mouse brain parts and stages (normalized against

actin), and 12 Drosophila tissues and stages

(normalized against rp49).

Real-Time Rapid-Scan is a new member of the Rapid-

Scan family. It is a real-time PCR ready assembly of

high-quality cDNAs from 48 human tissues and the

cDNA was normalized against beta-actin using real-

time PCR. There is no need for serial dilution

therefore only one concentration of (approximately

1ng) of cDNA is provided. The customer can simply

add the real-time PCR mix with the gene specific

primers and obtain an accurate gene expression

profile across 48 tissues with minimal hand-on time.

PRODUCT COMPONENTS

Two identical Rapid-Scan™ 48- or 96-well PCR plates containing dried, serially-diluted, PCR-ready,

first-strand cDNAs

Two adhesive cover sheetsfor sealing the PCR plates

Primers for detection ofcontrol cDNA

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4 Document the results by

imaging/photography.

The Rapid-Scan™ Gene Expression

Panels are easy to use and require a

minimal amount of time to obtain a

complete expression profile for a

given gene.

2 Place the plate

into a thermal

cycler and

proceed with

PCR.

3 Upon completion of the PCR, analyze

the reaction products by agarose gel

electrophoresis.

Product Use

1 Prepare a 48-well or 96-well PCR

master mix including two gene-

specific primers for the cDNA of

interest and aliquot the solution

into each well of the Rapid-Scan™

PCR plate.

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Rapi

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Product Application

A. Human Tissue Rapid-Scan™ Panel

The Human Rapid-Scan™ Gene Expression Panel

consists of cDNAs derived from 24 different tissues.

The cDNAs were arrayed in a 96-well PCR plate in the

order indicated in Fig. 1.

To facilitate comparison of transcript accumulation in

different tissues, the first-strand cDNAs have been

normalized using actin as an internal control.

Amplification of actin cDNA (using primers provided

with the Rapid-Scan™ panels) shows equivalent

distribution in all 24 human tissues (Fig. 2).

The Rapid-Scan™ panel was used to determine the

relative accumulation of the muscle-specific a-actinin

2 transcript, which is detected predominantly in

skeletal muscle and heart, but also in brain.

The Human Rapid-Scan™ panel was used to

generate expression profiles of selected previously-

known genes in various tissues or differentiation

states. Myelin-associated glycoprotein (MAG) was

detected only in adult brain, adenosine mono-

phosphate deaminase 1 (isoform 1) (AMPD1) only in

muscle, thyroglobulin (TG) only in thyroid gland,

serine protease 3 (mesotrypsin) (PRSS3) only in

pancreas, and protein tyrosine phosphatase (PTPN1)

only in prostate gland.

B. Human Brain Rapid-Scan™ Panel

The Human Brain Rapid-Scan™ Gene Expression

Panel consists of cDNAs derived from 12 different

brain parts. The RNA was prepared and pooled from

two individuals. The cDNAs were normalized against

actin and arrayed in a 48-well PCR plate in the order

indicated in Fig. 3.

This panel was used to determine the relative

transcript levels of a gene family. Three members of

the human dopamine receptor family were analyzed

and the PCR products were separated in an agarose

gel (Fig. 4).

Fig. 1

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MAG

AMPD1

TG

PRSS3

PTPN1

α-actinin 2

actin

M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

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1. Brain2. Heart3. Kidney4. Spleen5. Liver6. Colon7. Lung8. Small Intestine

9. Muscle10. Stomach11. Testis 12. Placenta13. Salivary Gland14. Thyroid Gland15. Adrenal Gland16. Pancreas

17. Ovary18. Uterus19. Prostate Gland20. Skin21. PBL22. Bone Marrow23. Fetal Brain24. Fetal Liver

Fig. 2

1 2 3 4 5 6 7 8 9 10 11 12

13 14 15 16 17 18 19 20 21 22 23 24

Fig. 3

1 2 3 4 5 6 7 8 9 1 0 1 1 1 2

1. Frontal Lobe2. Temporal Lobe3. Cerebellum4. Hippocampus5. Substantia Nigra6. Caudate Nucleus

7. Amygdala8. Thalamus9. Hypothalamus

10. Pons11. Medulla12. Spinal Cord

A

B

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The D5 dopamine receptor was present at more or

less equal levels in all of the brain parts examined.

The D2 receptor also showed broad distribution but

at varying levels of accumulation. On the other hand,

the D1 receptor was only expressed in the caudate

nucleus, verifying results that have been previously

observed. The results also demonstrate equivalent

levels of actin in each normalized tissue.

C. Human Breast Cancer Rapid-Scan™ Panel

The Human Breast Cancer Rapid- Scan™ Gene

Expression Panel consists of cDNAs derived from 12-

normal and 12-tumor breast tissues. The normal

tissues were obtained by reduction mammaplasty

and microdissected to remove fat tissues. The tumor

tissues were from patients with carcinoma of the

breast. The cDNAs were normalized against actin and

arrayed in a 96-well PCR plate in the order indicated

in Fig. 5.

This panel is designed to examine the change in

gene expression of a candidate gene between nor-

mal and tumor tissues. The expression of 6 genes

related in breast cancer was examined using this

panel. (Fig. 6)

Genes 1, 2, and 3 were up-regulated in many breast

tumor tissues and undetectable in normal breast

tissues. In contrast, the expression of genes 4, 5, and

6 were down-regulated in many breast tumor tissues

and almost undetectable in normal breast tissues.

Fig. 4

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actin

D1

D2

D5

1 2 3 4 5 6 7 8 9 10 11 12

1. Normal Breast 12. Normal Breast 23. Normal Breast 34. Normal Breast 45. Normal Breast 56. Normal Breast 67. Normal Breast 78. Normal Breast 89. Normal Breast 9

10. Normal Breast 1011. Normal Breast 1112. Normal Breast 12

13. Breast Cancer 114. Breast Cancer 215. Breast Cancer 316. Breast Cancer 417. Breast Cancer 518. Breast Cancer 619. Breast Cancer 720. Breast Cancer 821. Breast Cancer 922. Breast Cancer 1023. Breast Cancer 1124. Breast Cancer 12

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Fig. 6

1000 X1000 X1000 X1000 X

1000 X

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actin

Gene 1

Gene 2

Gene 3

Gene 4

Gene 5

Gene 6

M 1 2 3 4 5 6 7 8 9 10 11 12 M 13 14 15 16 17 18 19 20 21 22 23 24

1 2 3 4 5 6 7 8 9 10 11 12

13 14 15 16 17 18 19 20 21 22 23 24

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D. Mouse Tissue Rapid-Scan™ Panel

The Mouse Rapid-Scan™ Gene Expression Panel

consists of cDNAs derived from 24 different tissues

or developmental stages. The adult tissues were

from outbred Swiss Webster mice, and the breast

tissues from outbred CD1 mice. The cDNAs were

normalized against actin and arrayed in a 96-well

PCR plate in the order indicated in Fig. 7.

The Rapid-Scan™ panel is an effective tool to obtain

the expression profile of an uncharacterized EST or

to confirm tissue-specific expression. The results

shown in Fig. 8 demonstrate such an application.

The Mouse Rapid-Scan™ panel was used to confirm

the expression profiles for a mouse heart-specific

gene (Tbx20) and a skin-specific EST sequence. The

heart-specific gene (Tbx20) was found to be

expressed in abundance in heart and at a lower level

in embryo at different stages of gestation. The skin-

specific EST sequence was found to be expressed

not only in skin, but also in stomach and in day 19

embryos.

E. Mouse Brain Rapid-Scan™ Panel

The Mouse Brain Rapid-Scan™ Gene Expression

Panel consists of cDNAs derived from 48 mouse

brain parts at five developmental stages. The brain

tissues were harvested from Outbred NIH Swiss

mice. The cDNAs were normalized against actin and

arrayed in a 96-well PCR plate in the order indicated

in Fig. 9.

This panel has been used to profile the expression of

mouse DLX-5 (Drosophila homolog Distal-less 5),

dopamine receptor DD3 and Hox3.1. The results of

Hox3.1 are shown in Fig. 10.

In embryonic stages, Hox3.1 is expressed in many

brain parts with a high level of expression in the

spinal cord. However, in adults, its expression is

restricted to the spinal cord. This piece of data

demonstrates the usefulness of this panel in

providing clues to understanding potential gene

functions.

1000 X

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actin

Tbx20

Skin-specific EST

M 1 2 3 4 5 6 7 8 9 10 11 12 M 13 14 15 16 17 18 19 20 21 22 23 24

Fig. 7

Fig. 8

1. Brain2. Heart3. Kidney4. Spleen5. Thymus6. Liver7. Stomach8. Small Intestine

9. Muscle10. Lung11. Testis 12. Skin13. Adrenal Gland14. Pancreas15. Uterus16. Prostate Gland

17. Embryo/8.5 day18. Embryo/9.5 day19. Embryo/12.5 day20. Embryo/19 day21. Breast/Virgin22. Breast/Pregnant23. Breast/Lactating24. Breast/Involuting

A

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F. Drosophila Tissue Rapid-Scan™ Panel

The Drosophila Rapid-Scan™ Gene Expression


tissues and developmental stages. The RNA was pre-

pared from Drosophila melanogaster Canton S

strain. The cDNAs were then arrayed in a 48-well PCR

plate in the order indicated in Fig. 11. This was used

to confirm the expression profiles of genes whose

sites of expression have already been established.

The results are shown in Fig. 12.

The expression of the actin genes in Drosophila is

developmentally regulated, therefore, rp49, the gene

encoding a ribosomal protein, was used as an

internal control to normalize each Drosophila cDNA.

The male-specific Fruitless transcript was only

detected in the adult male head. The alternatively-

Fig. 9

Embryo day 131. Telencephalon/

Diencephalon2. Mesencephalon

(Midbrain)3. Rhombencephalon

(Hindbrain)4. Spinal cord

Embryo day 155. Telencephalon6. Diencephalon7. Midbrain8. Pons9. Medulla

10. Spinal cord

Embryo day 1811. Frontal cortex12. Posterior cortex13. Entorhinal cortex

14. Olfactory bulb15. Hippocampus16. Striatum17. Thalamus18. Hypothalamus19. Midbrain20. Pons21. Medulla22. Spinal cord

Postnatal day 7 23. Frontal cortex24. Posterior cortex25. Entorhinal cortex26. Olfactory bulb27. Hippocampus28. Striatum29. Thalamus30. Hypothalamus31. Cerebellum

32. Midbrain33. Pons34. Medulla35. Spinal cord

Adult 5 week36. Frontal cortex37. Posterior cortex38. Entorhinal

cortex39. Olfactory bulb40. Hippocampus41. Striatum42. Thalamus43. Hypothalamus44. Cerebellum45. Midbrain46. Pons47. Medulla48. Spinal cord

A

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13 14 15 16 17 18 19 20 21 22 23 24

25 26 27 28 29 30 31 32 33 34 35 36

37 38 39 40 41 42 43 44 45 46 47 48

Fig. 11

1. Embryo/0–4 hr2. Embryo/4–8 hr3. Embryo/8–12 hr4. Embryo/12–24 hr5. 1st Instar6. 2nd Instar

7. 3rd Instar8. Pupae9. Male Head

10. Female Head11. Male Body12. Female Body

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spliced male form of Doublesex was detected mainly

in the 3rd instar and in male tissues, and is not

present in female tissues. The Eyeless transcript was

detected in whole embryos, starting from 4 hours,

and in adult head, but not in adult body.

1-12

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25-36

25-36

37-48

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Bicoid

Antennapedia

Eyeless

Ultrabithorax

Fruitless(male form)

Doublesex(male form)

Doublesex(female form)

1 2 3 4 5 6 7 8 9 10 11 12

Fig. 12

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F. Human Real-Time Rapid-Scan Panel

The Human Real-Time Rapid-Scan gene Expression


tissues. The cDNAs were arrayed in a 96 well PCR

plate in the order indicated in Fig. 13.

The first-strand cDNAs have been normalized by

real-time PCR using beta-actin as an internal control.

Amplification of actin cDNA (using primers provided

with the Rapid-Scan panels shows equivalent

distribution in all 48 human tissues. (Fig. 14, Panel A)

To validate the panel for specificity and sensitivity,

the human Real-Time Rapid-Scan Panel was used to

generate expression profiles of selected previously-

known gene in various tissues (Fig. 14, Panel B-D).

Thyroglobulin was detected only in thryroid and

parathyroid. Skin-specific gene, Late cornified

envelope 2B (LCE2B) was detected only in nasal

mucosa and skin. Prostate specific antigen, kallikrein

3 (KLK3) was detected only in prostate.

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48

Beta-actin

thyroglobulin

LCE2B

KLK3

A

B

C

D

FIG. 14

1 2 3 4 5 6 7 8 9 1 0 1 1 1 2

A

B

C

D

1 Adrenal Gland2 Bile duct3 Bone marrow4 Brain5 Bronchus6 Cervix7 Colon8 Decending part

of duodenum9 Epididymis

10 Esophagus11 Fat12 Heart

13 Intestine (Small)14 Intracranial artery15 Kidney16 Larynx17 Liver18 Lung19 Lymph node20 Lymphocytes

(peripheral blood)21 Mammary gland22 Muscle23 Nasal mucosa24 Optic nerve

25 Ovary26 Oviduct27 Pancreas28 Parathyroid29 Pericardium30 Pituitary31 Placenta32 Prostate33 Rectum34 Salivary Gland35 Seminal vesicles36 Skin

37 Spleen38 Stomach39 Testis40 Thymus41 Thyroid42 Tonsil43 Ureter44 Urinary bladder45 Uterus46 Uvula47 Vagina48 Vena Cava

Row A Row B Row C Row D

Fig. 13

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Product Listing Cat. No. PriceHuman Poly A+ RNA Blot I HB-2010 725Northern Blot — 12 Tissues(Brain, Lung, Heart, Muscle, Stomach, Small Intestine, Colon, Liver, Kidney, Spleen, Testis, Placenta)

Human Poly A+ RNA Blot II HB-2011 725Northern Blot — 12 Tissues(Brain, Duodenum, Esophagus, Pancreas, PBL, Prostate, Salivary Gland, Testis, Thymus, Thyroid, Urinary Bladder, Uterus )

Human Poly A+ RNA Blot III HB-1102 415Northern Blot — 6 Tissues(Salivary Gland, Pancreas, Adrenal Gland, Ovary, Uterus, Skin)

Mouse Poly A+ RNA Blot MB-2020 640Northern Blot — 12 Major Tissues(Brain, Lung, Heart, Muscle, Stomach, Small Intestine, Liver, Kidney, Spleen, Testis, Thymus, Skin)

Rat Poly A+ RNA Blot RB-2030 640Northern Blot — 12 Major Tissues(Brain, Thymus, Lung, Heart, Muscle, Stomach, Small Intestine, Liver, Kidney, Spleen, Testis, Skin)

Rat Total RNA Blot SB-2040 710Northern Blot — 12 Brain Parts (Frontal Cortex, Posterior Cortex, Entorhinal Cortex, Hippocampus, Olfactory Bulb, Striatum, Thalamus, Cerebellum, Midbrain, Pons, Medulla, Spinal Cord)

Northern blot

Introduction

Identification of tissues which express a gene of

interest is an essential first step for functional

analysis. While the Rapid-Scan™ Gene Expression

Panel is a highly sensitive and time-efficient system

to measure specific transcript accumulation, it does

not provide information about the size of the

transcript and is only semi-quantitative. The

workhorse in the industry, RNA blot hybridization or

Northern blotting, has been widely used for gene

expression analysis. Though often laborious,

Northern blotting provides, other than determination

of sites of expression, information regarding tran-

script size, RNA quantification, cross-hybridization

data, and alternate RNA splicing. In the standard

method, isolated total or poly A+ RNA from a variety

of tissues are separated by gel electrophoresis and

transferred onto a solid support. Hybridization of the

immobilized RNA with a labeled nucleic acid probe is

then performed to determine the relative levels of an

mRNA species in the various tissues.

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Since 1997, OriGene has been providing pre-made

Northern blots to the research community. Its high

quality and reliability has been validated by citations

in many publications in peer-reviewed journals. The

citation list below is only a small sample of such

publications.

Product Description

The Northern Blots are premade, ready-to-hybridize

membranes offered in a variety of choices, in terms

of organism, tissue, and type of RNA. The integrity of

the RNA used in the blots is assured through a rigid

quality control process. Approximately 2 µg of

poly A+ RNA or 20 µg of total RNA per tissue sample

were separated in a denaturing agarose gel,

transferred onto a positively-charged nylon

membrane, and covalently crosslinked by UV light

irradiation. Loading of the poly A+ RNA from each

tissue was adjusted to reflect approximately equal

amounts of actin mRNA, while total RNA samples

were adjusted to reflect equivalent levels of

ribosomal RNA. Signal intensity for actin across each

tissue sample may vary slightly. Each blot contains

RNA markers for transcript size determination.

OriGene offers human RNA blots prepared from 12

tissues (Blot I and Blot II) or six tissues (Blot III).

OriGene also offers a mouse 12-major tissue blot, a

rat 12-major tissue blot, and a rat 12-brain part blot.

1. Cloning and characterization of mouse E2F8, a novel mammalian E2F family membercapable of blocking cellular proliferation.J Biol Chem. 2005 Feb 18.2. A targeted mutation of Nkd1 impairs mouse spermatogenesis.J Biol Chem. 2005 Jan 28;280(4):2831-9.3. dsu functions in a MYO5A-independent pathway to suppress the coat color of dilutemice.Proc Natl Acad Sci U S A. 2004 Nov 30;101(48):16831-6.4. Expression of slc5a8 in kidney and its role in Na(+)-coupled transport of lactate.J Biol Chem. 2004 Oct 22;279(43):44522-32.5. Targeted disruption of the Walker-Warburg syndrome gene Pomt1 in mouse results inembryonic lethality.Proc Natl Acad Sci U S A. 2004 Sep 28;101(39):14126-31.

6. Neonatal lethality, dwarfism, and abnormal brain development in Dmbx1 mutant mice.Mol Cell Biol. 2004 Sep;24(17):7548-58.7. TNAP, a novel repressor of NF-kappaB-inducing kinase, suppresses NF-kappaBactivation.J Biol Chem. 2004 Aug 20;279(34):35975-83. 8. A novel cardiolipin-remodeling pathway revealed by a gene encoding an endoplasmicreticulum-associated acyl-CoA:lysocardiolipin acyltransferase (ALCAT1) in mouse.J Biol Chem. 2004 Jul 23;279(30):31727-34.9. An inhibitory Ig superfamily protein expressed by lymphocytes and APCs is also anearly marker of thymocyte positive selection.J Immunol. 2004 May 15;172(10):5931-9.

ADVANTAGES

12-tissue Northern for human, mouse and rat

Double-purified poly A+ RNA and rigorous QC standards

More RNA than other commercial blots

Everyday low price

Trusted by scientists and proven by numerous citations

Northern Blots eliminate the laborious

need to isolate, separate and transfer

tissue RNA. Quality Northern blots are

obtained by following standard

hybridization conditions and using a

labeled nucleic acid probe.

2 Hybridization

with probe

3 Washing of blot4 Autoradiography/phosphorimaging

1 Pre-hybridization

Product Use

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Product Application

A. Human Northern Blots

The human blots are prepared from polyA+ RNAs.

Three different blots covering 22 clinically relevant

tissues are provided.

• Blot I: 12-tissue poly A+ RNA blot (brain, heart,

kidney, lung, small intestine, muscle, spleen, liver,

colon, stomach, testis, placenta)

• Blot II: 12-tissue poly A+ RNA blot (brain,

duodenum, esophagus, pancreas, PBL, prostate,

salivary gland, testis, thymus, thyroid, urinary

bladder, uterus)

• Blot III: 6-tissue poly A+ RNA blot (pancreas,

adrenal gland, ovary, salivary gland, uterus, skin)

OriGene’s pre-made Northern are prepared with

highly enriched polyA+ RNA. The RNA was double

purified by oligo-dT affinity columns. All blots are

normalized by actin expression level. In Fig 1,

HB2010 and HB2011 was hybridized with beta-actin

probe. (Muscle and heart contain shorter alpha actin

that cross-hybridize with beta-actin probe).

As an indication of the quality of the Northern blots,

a human 12-tissue blot I was successively hybridized,

stripped, and re-hybridized to one of three 32P-

labeled, PCR-derived, DNA probes (each about 0.5 kb

in length) (Fig. 2). The three probes used were actin

(panel A), carbonic anhydrase (panel B), and α-

actinin 2 (panel C).

All 12-tissues RNAs contain relatively equal levels of

intact actin mRNA (α-actin in heart and muscle, and

β-actin in the other tissues) (panel A). Low

abundance and tissue-specific transcripts, such as

that of carbonic anhydrase (panel B), are easily

detected. Moreover, closely-related sequences, such

as that of the 4.2-kb α-actinin 2 and 2.9-kb, α-actinin

3 transctipts, can be detected through cross-

hybridization of the probe (panel C). Though less

sensitive than the Rapid-Scan panels, Northern blots

are quantitative and provide information about

transcript sizes and cross-hybridization of mRNA

species.

A

B

C

— 1.8 kb

— 2.4 kb

— 4.2 kb

— 2.9 kb

Fig. 2

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in

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L

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is,T

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Uri

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in

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Sm

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beta-actin

alpha-actin

Cat#: HB2010 Cat#: HB2011

Fig. 1

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B. Mouse Northern Blots

The mouse blots are prepared from poly A+ RNA from

12 major tissues.

• 12-major tissue poly A+ RNA (brain heart,

kidney, liver, lung, muscle, skin, small intestine,

spleen, stomach, testis, thymus)

As an indication of the quality of the Northern blots,

a mouse 12-major tissue blot was successively

hybridized, stripped, and re-hybridized to one of

three 32P-labeled, PCR-derived, DNA probes (each

about 0.5 kb in length), which represent four different

“housekeeping” genes (Fig. 3). The four probes used

were actin (panel A), GAPDH (panel B), a calcium-

binding protein Cab45a (panel C), and phospholipase

A2 (panel D).

The results demonstrate that the blot contains fully

intact RNA and that the actin signal is generally

equal across the blot. Also, it is possible for specific

transcript signals to be effectively normalized

against other “housekeeping” genes in addition to

actin, to which the poly A+ RNA on the blot was

standardized. The relative abundance of a specific

mRNA can be measured against GAPDH,

phospholipase A2, or Cab45a, by simply using the

signal ratio of actin to either of these transcripts and

thus leading to a re-normalization of the signal.

Fig. 3

A

B

C

D

— 1.8 kb

— 1.2 kb

— 2.4 kb

— 5.0 kb

— 2.3 kb

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C. Rat Northern Blots

The rat blots are prepared from either poly A+ RNA

from various major tissues or total RNA from

different brain parts.

• 12 major-tissue poly A+ RNA blot (brain,

thymus, lung, heart, muscle, stomach, small

intestine, liver, kidney, spleen, testis, skin)

• 12 brain-part total RNA blot (frontal cortex,

posterior cortex, entorhinal cortex,

hippocampus, olfactory bulb, striatum,

thalamus, cerebellum, midbrain, pons,

medulla, spinal cord)

When the rat 12 major-issue blot was hybridized with

a 32P-labeled actin probe (Fig. 4), the result shows

equal RNA loading in each of the lanes and

intactness of each of the RNAs.

Frontal Cortex

Posterior Cortex

Hippocampus

Olfactory Bulb

Striatum

Thalamus

Cerebellum

Midbrain

Entorhinal Cortex

Medulla

Spinal Cord

Pons

Fig. 4

— 1.8 kb

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Multiple-Choice™ cDNA sets (10 reactions)

Human Multiple-Choice™ First-Strand cDNA Set 1 CH-1101 395(Brain, Heart, Kidney, Spleen, Liver, Peripheral Blood Leukocytes)

Human Multiple-Choice™ First-Strand cDNA Set 2 CH-1102 395(Small Intestine, Muscle, Lung, Prostate, Testis, Ovary)

Human Multiple-Choice™ First-Strand cDNA Set 3 CH-1103 395(Fetal Brain, Fetal Kidney, Fetal Testis, Fetal Liver, Fetal Muscle, Fetal Spleen)

Mouse Multiple-Choice™ First-Strand cDNA Set 1 CM-1101 395(Brain, Heart, Kidney, Spleen, Thymus, Liver)

Mouse Multiple-Choice™ First-Strand cDNA Set 2 CM-1102 395(Stomach, Small Intestine, Muscle, Lung, Testis, Skin)

Rat Multiple-Choice™ First-Strand cDNA Set 1 CR-1101 395(Brain, Heart, Kidney, Spleen, Thymus, Liver)

Rat Multiple-Choice™ First-Strand cDNA Set 2 CR-1102 395(Stomach, Small Intestine, Muscle, Lung, Testis, Skin)

Human First-Strand cDNA (30 reactions)

Human Brain CH-1001 295

Human Heart CH-1002 295

Human Kidney CH-1003 295

Human Liver CH-1005 295

Human Lung CH-1009 295

Human Muscle CH-1008 295

Human Ovary CH-1012 295

Human Peripheral Blood Leukocytes CH-1006 295

Human Placenta CH-1014 295

Multiple-Choice™ cDNAs

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Human Prostate CH-1010 295

Human Small Intestine CH-1007 295

Human Spleen CH-1004 295

Human Stomach CH-1013 295

Human Testis CH-1011 295

Human Fetal Brain CH-1015 295

Human Fetal Kidney CH-1016 295

Human Fetal Liver CH-1018 295

Human Fetal Muscle CH-1019 295

Human Fetal Spleen CH-1020 295

Human Fetal Testis CH-1017 295

Mouse First-Strand cDNA (30 reactions)

Mouse Brain CM-1001 295

Mouse Heart CM-1002 295

Mouse Kidney CM-1003 295

Mouse Liver CM-1006 295

Mouse Lung CM-1010 295

Mouse Muscle CM-1009 295

Mouse Skin CM-1012 295

Mouse Small Intestine CM-1008 295

Mouse Spleen CM-1004 295

Mouse Stomach CM-1007 295

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Mouse Testis CM-1011 295

Mouse Thymus CM-1005 295

Rat First-Strand cDNA (30 reactions)

Rat Brain CR-1001 295

Rat Heart CR-1002 295

Rat Kidney CR-1003 295

Rat Liver CR-1006 295

Rat Lung CR-1010 295

Rat Muscle CR-1009 295

Rat Skin CR-1012 295

Rat Small Intestine CR-1008 295

Rat Spleen CR-1004 295

Rat Stomach CR-1007 295

Rat Testis CR-1011 295

Rat Thymus CR-1005 295

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Introduction

Amplification of cDNA by PCR is an essential tool for

today’s molecular biologist. This technique can be

used to clone members of a multi-gene family,

homologous genes from different species, and genes

previously identified. PCR amplification of Multiple-

Choice™ First-Strand cDNA and its subsequent

subcloning can often save time and reduce the cost

by eliminating the need to construct and screen a

panel of tissues for the expression of your gene of

interest. Multiple-Choice™ First-Strand cDNA is also

ideal for the characterization of alternatively spliced

mRNA either in a single tissue or across tissues in an

organism.

Product Description

Multiple-Choice™ cDNA is a PCR-ready, first-strand

cDNA prepared from human, mouse or rat. Multiple-

Choice™ First-Strand cDNA is synthesized from

poly A+ RNA, using an oligo(dT) primer and a reverse

transcriptase that favors the production of long

products. It is provided at a concentration of

2-10 ng/µL.

Multiple-Choice™ First-Strand cDNA is available in

two formats:

• Set of six-tissue cDNAs

• Individual tissue cDNA

Product Application

When Multiple-Choice™ cDNA is used as a template

for PCR, it results in specific amplification of the

desired sequence. For example, as shown in Fig. 1,

an actin signal is detectable in each cDNA sample of

the rat Multiple-Choice™ cDNA Set 1, whereas, the

liver-specific cytochrome P450 can only be amplified

from the liver cDNA sample.

ADVANTAGES

Synthesized from high quality poly A+ RNA

Oligo(dT)-primed reverse transcribed

Available as sets of six cDNAs

Control PCR primers included

ActinLiver-Specific

Cytochrome P450

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Human Blue-Ribbon™ RNA

Human Brain, total, 250 µg HT-1001 245

Human Brain, poly A+, 5 µg HM-1001 320

Human Colon, total, 250 µg HT-1015 245

Human Colon, poly A+, 5 µg HM-1015 320

Human Heart, total, 250 µg HT-1002 245

Human Heart, poly A+, 5 µg HM-1002 320

Human Kidney, total, 250 µg HT-1003 245

Human Kidney, poly A+, 5 µg HM-1003 320

Human Liver, total, 250 µg HT-1005 245

Human Liver, poly A+, 5 µg HM-1005 320

Human Lung, total, 250 µg HT-1009 245

Human Lung, poly A+, 5 µg HM-1009 320

Human Muscle, total, 250 µg HT-1008 245

Human Muscle, poly A+, 5 µg HM-1008 320

Human Placenta, total, 250 µg HT-1013 245

Human Placenta, poly A+, 5 µg HM-1013 320

Human Small Intestine, total, 250 µg HT-1007 245

Human Small Intestine, poly A+, 5 µg HM-1007 320

Human Spleen, total, 250 µg HT-1004 245

Human Spleen, poly A+, 5 µg HM-1004 320

Human Stomach, total, 250 µg HT-1014 245

Blue-Ribbon™ Poly A+ and total RNA

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Human Stomach, poly A+, 5 µg HM-1014 320

Human Testis, total, 250 µg HT-1011 245

Human Testis, poly A+, 5 µg HM-1011 320

Mouse Blue-Ribbon™ RNA

Mouse Brain, total, 250 µg MT-1001 145

Mouse Brain, poly A+, 5 µg MM-1001 165

Mouse Heart, total, 250 µg MT-1002 145

Mouse Heart, poly A+, 5 µg MM-1002 165

Mouse Kidney, total, 250 µg MT-1003 145

Mouse Kidney, poly A+, 5 µg MM-1003 165

Mouse Liver, total, 250 µg MT-1006 145

Mouse Liver, poly A+, 5 µg MM-1006 165

Mouse Lung, total, 250 µg MT-1010 145

Mouse Lung, poly A+, 5 µg MM-1010 165

Mouse Muscle, total, 250 µg MT-1009 145

Mouse Muscle, poly A+, 5 µg MM-1009 165

Mouse Skin, total, 250 µg MT-1012 145

Mouse Skin, poly A+, 5 µg MM-1012 165

Mouse Small Intestine, total, 250 µg MT-1008 145

Mouse Small Intestine, poly A+, 5 µg MM-1008 165

Mouse Spleen, total, 250 µg MT-1004 145

Mouse Spleen, poly A+, 5 µg MM-1004 165

Mouse Stomach, total, 250 µg MT-1007 145

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Mouse Stomach, poly A+, 5 µg MM-1007 165

Mouse Testis, total, 250 µg MT-1011 145

Mouse Testis, poly A+, 5 µg MM-1011 165

Mouse Thymus, total, 250 µg MT-1005 145

Mouse Thymus, poly A+, 5 µg MM-1005 165

Rat Blue-Ribbon™ RNA

Rat Brain, total, 250 µg RT-1001 145

Rat Brain, poly A+, 5 µg RM-1001 165

Rat Heart, total, 250 µg RT-1002 145

Rat Heart, poly A+, 5 µg RM-1002 165

Rat Kidney, total, 250 µg RT-1003 145

Rat Kidney, poly A+, 5 µg RM-1003 165

Rat Liver, total, 250 µg RT-1006 145

Rat Liver, poly A+, 5 µg RM-1006 165

Rat Lung, total, 250 µg RT-1010 145

Rat Lung, poly A+, 5 µg RM-1010 165

Rat Muscle, total, 250 µg RT-1009 145

Rat Muscle, poly A+, 5 µg RM-1009 165

Rat Skin, total, 250 µg RT-1012 145

Rat Skin, poly A+, 5 µg RM-1012 165

Rat Small Intestine, total, 250 µg RT-1008 145

Rat Small Intestine, poly A+, 5 µg RM-1008 165

Rat Spleen, total, 250 µg RT-1004 145

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Rat Spleen, poly A+, 5 µg RM-1004 165

Rat Stomach, total, 250 µg RT-1007 145

Rat Stomach, poly A+, 5 µg RM-1007 165

Rat Testis, total, 250 µg RT-1011 145

Rat Testis, poly A+, 5 µg RM-1011 165

Rat Thymus, total, 250 µg RT-1005 145

Rat Thymus, poly A+, 5 µg RM-1005 165

Introduction

RNA preparations from animal tissues can be very

useful in a variety of molecular biology techniques

including Northern blot analysis, RT-PCR, and cDNA

synthesis Blue-Ribbon™ Total and Poly A+ RNA are

available as prepared from either human, mouse, or

rat tissues.

Product Description

Both total RNA or poly A+ RNA samples are available

from 12 major tissues of human, mouse, or rat. Total

RNA is isolated directly from fresh or quick-frozen

tissues to ensure the highest quality products. Total

RNA contains intact 28S and 18S ribosomal RNA

bands as determined by denaturing agarose gel

electrophoresis. Poly A+ RNA is isolated from intact

total RNA using oligo(dT). The isolation method yields

high-quality poly A+ RNA. The RNA is examined by

denaturing agarose gel electrophoresis to ensure that

high molecular weight mRNA is intact. Both total RNA

and poly A+ RNA are supplied in DEPC-treated water.

Quality Control

• All RNA is checked by agarose gel electrophoresis

• Each lot of RNA is Northern blotted and probed

with actin

Blue-Ribbon™ RNA is available in two formats:

• Total RNA (250 µg)

• Poly A+ RNA (5 µg)

ADVANTAGES

High quality total RNA or poly A+ RNA

Choice of 12 major tissues

Human, mouse, or rat

Miscellaneous molecular toolsPre-validated antibodies

Protein collections

Quanti-Ladder™:Broad range DNA size marker (200bp-10kb) with quantity reference

Millennium™ marker:Broad range RNA size marker (0.5kb-9kb)

Rapid-Load™:Innovative PCR compatible loading buffer

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Pre-validated Antibodies

Introduction

The OriGene Antibody Collections have been

obtained from suppliers that adhere to the highest

quality standards. Currently OriGene’s antibody

coverage is focused on protein kinases and G protein

coupled receptors (GPCR). These antibodies, pre-

validated for specificity and sensitivity, provide the

convenient and reliable sets of tools for gene

expression and activity monitoring.

Production begins with extensive purification (typ-

ically by both protein A and peptide affinity

chromatography) followed by testing on a wide range

of applications, including ELISA, western blotting,

immunoprecipitation, immunocytochemistry, immu-

nohistochemistry and flow cytometry. For each anti-

body, the QC data is posted on the website for

customer’s evaluation.

All antibodies can be searched by:



• Protein Domain

• Keywords

• Gene Family

Product Application

The specificity of the antibody-antigen interaction is

well exploited in laboratory experimentation.

Additionally, the sensitivity of detection for antibody

recognition also drives the variety of applications

listed below. Not all antibodies are qualified for all

applications. Please consult the individual product

specifications.

Western Blots: Proteins from cellular extracts can be

separated through SDS-PAGE and transferred to a

host of solid-surface membranes. Antibodies for the

specific recognition of proteins in limiting amounts

can then be used to probe these membranes.

Immunoprecipitation (IP) or Radioimmunoassay

(RAI):The binding of antibodies to antigens in solution

permits both the purification of antigens (IP) and the

identification / quantification of antigens within a

given sample (RAI).

Immunohistochemistry (IHC) or Immunocytochem-

istry (IC): The molecular characterization of pathology

sections or tissue samples can be addressed by

antibody binding. Antibodies can differentiate cell

types as well as protein expression levels. These are

semi-quantitative approaches that are used to

diagnose disease states or tissue types.

Flow cytometry: Cells presenting specific cell-

surface proteins can be counted via the specific

recognition of antibodies to these antigens. This is

particularly useful in the diagnosis of different cell

types in peripheral blood samples.

Functional studies: Antibodies can be used to block

the function of proteins or other antigens through

steric interference within binding sites or points of

substrate interactions. One can neutralize both

enzyme activity and other types of protein-protein

interactions.

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Cat. # Description Format Application Host Sp Cross Reactivity Type Price*

TA201002 BID Antibody (Human Specific) 100 µL (10 WB) W IP Rabbit H Polyclonal 150

TA201003 BID Antibody (Mouse Specific) 100 µL (10 WB) W IP Rabbit M Polyclonal 150

TA201004 BID (7A3) Mouse mAb (Human Specific 100 µL (10 WB) W Mouse H Monoclonal 195

TA201005 BID (7A3) Mouse mAb (Human Specific 300 µL (30 WB) W Mouse H Monoclonal 475

TA201006 IL-1beta Antibody 100 µL (10 WB) W Rabbit H Polyclonal 150

TA201007 Lamin A/C Antibody 100 µL (10 WB) W IHC IC Rabbit H M R Polyclonal 150

TA201008 XIAP Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150

TA201009 PKD/PKCmu Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150

TA201010 Histone Deacetylase 1 (HDAC1) Antibody 100 µL (10 WB) W IP IHC IC Rabbit H M R Mk Polyclonal 150

TA201011 Histone Deacetylase 4 (HDAC4) Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150

TA200491 Neurotensin Receptor Type 1 1 mg/ml IHC Rabbit polyclonal 300

TA200061 G Protein-Coupled Receptor TM7XN1/GPR56 1 mg/ml IHC Rabbit polyclonal 350

TA200085 Melanocortin 4 Receptor (MC4R) 1 mg/ml IHC, ICC Rabbit polyclonal 300

TA201014 Parkin Antibody 100 µL (10 WB) W IP IC Rabbit H M R Polyclonal 150

TA201015 WSTF Antibody 100 µL (10 WB) W IP IC Rabbit H M R Mk Polyclonal 150

TA201016 Histone Deacetylase 6 (HDAC6) Antibody 100 µL (10 WB) W IHC IC Rabbit H M R Mk Polyclonal 150

TA201017 DAP3 Antibody 100 µL (10 WB) W Rabbit H Polyclonal 150

TA201018 DAP5 Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150

TA201019 Presenilin 2 Antibody 100 µL (10 WB) W IP IHC IC Rabbit H M R Mk Polyclonal 150

TA201020 Caspase-12 Antibody 100 µL (10 WB) W IC Rabbit M Polyclonal 150

TA201021 S6 Ribosomal Protein Antibody 100 µL (10 WB) W E IHC IC F Rabbit H M R Mk Polyclonal 150

TA201022 S6 Ribosomal Protein (5G10) Rabbit mAb 100 µL (10 WB) W IHC Rabbit H M R Mk Monoclonal 175

TA201023 EGF Receptor Antibody 100 µL (10 WB) W IP IHC IC F Rabbit H M R Polyclonal 150

TA200270 G Protein-Coupled Receptor GPR58 1 mg/ml IHC Rabbit polyclonal 350



TA200111 Thyrotropin-Releasing Hormone Receptor (TRH-R) 1 mg/ml IHC Rabbit polyclonal 300

TA201024 EGF Receptor (1F4) Mouse mAb 100 µL (10 WB) W IHC Mouse H Monoclonal 150

TA201025 HER2/ErbB2 Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Polyclonal 150

TA201026 HER2/ErbB2 (44E7) Mouse mAb 100 µL (10 WB) W IC Mouse H Monoclonal 150

TA201027 Skb1Hs Methyltransferase Antibody 100 µL (10 WB) IP W Rabbit H M R Mk Polyclonal 150

TA201028 Myc-Tag (9B11) Mouse mAb 0.2 milligrams W IP IC Rabbit ALL Monoclonal 150

TA201029 DAP1 Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150

TA201030 DARPP-32 Antibody 100 µL (10 WB) W IHC Rabbit H M R Polyclonal 150

TA201031 Synapsin Antibody 100 µL (10 WB) W Rabbit M R (H) Polyclonal 150

TA201032 eEF2 Antibody 100 µL (10 WB) W IC Rabbit H M R (C) (Hm) Polyclonal 150

TA201033 Dynamin I/II Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Polyclonal 150

TA201034 Chk1 Antibody 100 µL (10 WB) W Rabbit H R Mi Mk Polyclonal 150

TA200112 Glucagon-Like Peptide 1 Receptor 1 mg/ml IHC Rabbit polyclonal 350


TA200263 Prokineticin Receptor 2 (GPR73B) 1 mg/ml IHC Rabbit polyclonal 350

TA201035 His-Tag Polyclonal Antibody 100 µL (10 WB) W IP IC Rabbit ALL Polyclonal 150

TA201036 His-Tag (27E8) Mouse mAb 100 µL (10 WB) W IP IC Mouse ALL Monoclonal 150

TA201037 HA-Tag (6E2) Mouse mAb 100 µL (10 WB) W IP IHC IC F Mouse ALL Monoclonal 150

TA201038 Beta-Gal (14B7) Mouse mAb 0.2 milligrams W IP IC Mouse ALL Monoclonal 150

TA201039 IRS-1 Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Polyclonal 150

Product offering is subject to change. Restricted availability to international customers. Refer to website for the most updated information.

Sp. Cross Reactivity Key: H=Human M=Mouse Mi=Mink Mk=Monkey R=Rat C=Chicken Hm=Hamster X=Xenopus Z=Zebra Fish

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TA201040 MBP (8G1) Mouse mAb 0.2 milligrams W IP IC Mouse ALL Monoclonal 150

TA201041 HSP27 (G31) Mouse mAb 100 µL (10 WB) W IHC IC Mouse H Mk Monoclonal 150

TA201042 Shc Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150

TA201043 APP Antibody 100 µL (10 WB) W IHC Rabbit H M R Mk Polyclonal 150

TA201044 beta-Amyloid Antibody 100 µL (10 WB) W Rabbit H (R) Polyclonal 150

TA201045 VEGF Receptor 2 Antibody 100 µL (10 WB) W Rabbit H (M) (R) Polyclonal 150

TA201046 PLCbeta3 Antibody 100 µL (10 WB) W IHC IC Rabbit H M R Mk Polyclonal 150

TA200301 Vasopressin V1B Receptor 1 mg/ml IHC Rabbit polyclonal 200

TA200333 G Protein-Coupled Receptor JEG18 1 mg/ml IHC Rabbit polyclonal 350

TA200336 G Protein-Coupled Receptor JEG18 1 mg/ml IHC Rabbit polyclonal 350

TA201047 PPARgamma Antibody 100 µL (10 WB) W IHC Rabbit H M (R) Polyclonal 150

TA201048 Vav Antibody 100 µL (10 WB) W IP IHC Rabbit H R Polyclonal 150

TA201049 Estrogen Receptor alpha (62A3) Mouse mAb 100 µL (10 WB) W Mouse H Monoclonal 150

TA201050 p53 (1C12) Mouse mAb 100 µL (10 WB) W IP IHC IC Mouse H M R Mk Monoclonal 150

TA201051 AMPK-alpha Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150

TA201052 Paxillin Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150

TA201053 p27 Kip1 Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Polyclonal 150

TA201054 Histone H2A Antibody 100 µL (10 WB) W IP IC Rabbit H M R Mk Polyclonal 150

TA201055 PP1alpha Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150

TA201056 Histone H4 Antibody 100 µL (10 WB) W IP IHC IC Rabbit H M R Mk Polyclonal 150

TA201057 PAK1 Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Mk Polyclonal 150

TA200351 Glucagon Receptor 1 mg/ml IHC Rabbit polyclonal 250

TA200355 G Protein-Coupled Receptor C5L2 1 mg/ml IHC Rabbit polyclonal 350

TA200356 G Protein-Coupled Receptor C5L2 1 mg/ml IHC Rabbit polyclonal 350

TA201058 PAK1/2/3 Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150

TA201059 PAK2 Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150

TA201060 PAK3 Antibody 100 µL (10 WB) W IP Rabbit H M (R) Polyclonal 150

TA201061 PRK2 Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150

TA201062 GST Antibody 100 µL (10 WB) W IP IC Rabbit All Polyclonal 150

TA201063 GST (26H1) Mouse mAb 0.2 milligrams W IC Mouse All Monoclonal 150

TA201064 Histone Deacetylase 3 (HDAC3) Antibody 100 µL (10 WB) W IHC IC Rabbit H M R Mk Polyclonal 150

TA201065 Alpha-Synuclein Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150

TA201066 Chk2 Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150

TA201067 IKKalpha Antibody 100 µL (10 WB) W IP IC Rabbit H M R Polyclonal 150

TA201068 IKKbeta Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150

TA201069 IKKgamma Antibody 100 µL (10 WB) W IHC Rabbit H Mk M R Polyclonal 150

TA201070 IKK epsilon Antibody 100 µL (10 WB) W IP Rabbit H Polyclonal 150

TA201071 Zap-70 (99F2) Rabbit mAb 100 µL (10 WB) W IP IHC F Rabbit H M Monoclonal 150

TA201072 Syk Antibody 100 µL (10 WB) W IC Rabbit H M Polyclonal 150

TA201073 Histone H2B Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150

TA201074 Lyn Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Polyclonal 150

TA201075 Lck Antibody 100 µL (10 WB) W IP IHC F Rabbit H M Polyclonal 150

TA201076 Bcl-xL Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Mk Polyclonal 150

TA201077 Bax Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150

TA201078 FADD Antibody (Human Specific) 100 µL (10 WB) W Rabbit H Polyclonal 150

TA201079 Tyrosine Hydroxylase Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150



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TA201080 Survivin (6E4) Mouse mAb 100 µL (10 WB) W Rabbit H Mk Monoclonal 150

TA201081 MLK3 Antibody 100 µL (10 WB) W Rabbit H M R Hm Mk Polyclonal 150

TA201082 PLCgamma1 Antibody 100 µL (10 WB) W IP Rabbit H M (R) Polyclonal 150

TA201083 cPLA2 Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150

TA201084 c-Abl Antibody 100 µL (10 WB) W IP IHC F IC Rabbit H M R Polyclonal 150

TA201085 Bcl-2 Antibody 100 µL (10 WB) W IP IHC Rabbit H Polyclonal 150

TA201086 Histone Deacetylase 7 (HDAC7) Antibody 100 µL (10 WB) W IP IC Rabbit H M R Polyclonal 150

TA201087 p18 INK4C (DCS118) Mouse mAb 100 µL (10 WB) W IP Mouse H Monoclonal 150

TA201088 cdk4 (DCS156) Mouse mAb 100 µL (10 WB) W IHC Mouse H M Monoclonal 150

TA201089 cdk7 (MO1) Mouse mAb 100 µL (10 WB) W IP IHC IC Mouse H R Monoclonal 150

TA201090 Cyclin D1 (DCS6) Mouse mAb 100 µL (10 WB) W IP IHC F Mouse H M R Monoclonal 150

TA201091 Cyclin D3 (DCS22) Mouse mAb 100 µL (10 WB) W IHC Mouse H M R Monoclonal 150

TA200001 Beta-2 Adrenoceptor 1 mg/ml IHC, ICC Rabbit polyclonal 300

TA200002 Endothelin B Receptor 1 mg/ml IHC, ICC Rabbit polyclonal 300

TA200003 Bradykinin B2 Receptor 1 mg/ml IHC, ICC Rabbit polyclonal 300

TA200004 Interleukin 8 Receptor A 1 mg/ml IHC, ICC Rabbit polyclonal 300

TA200005 C-C Chemokine Receptor 2 (CCR2) 1 mg/ml IHC Rabbit polyclonal 300

TA200006 Adenosine A2a Receptor 1 mg/ml IHC, ICC Rabbit polyclonal 200

TA200007 Muscarinic Acetylcholine Receptor M3 1 mg/ml IHC Rabbit polyclonal 300


TA200009 Dopamine Receptor D1 1 mg/ml IHC, ICC Rabbit polyclonal 300

TA200010 Dopamine Receptor D1 1 mg/ml IHC, ICC Rabbit polyclonal 300

TA200011 Metabotropic Glutamate Receptor 1 1 mg/ml IHC Rabbit polyclonal 300

TA200012 Metabotropic Glutamate Receptor 2 1 mg/ml IHC, ICC, W Rabbit polyclonal 300

TA200013 Metabotropic Glutamate Receptor 3 1 mg/ml IHC, ICC Rabbit polyclonal 300









TA200018 5-HT4 Receptor 1 mg/ml IHC Rabbit polyclonal 250

TA200020 Prostaglandin E Receptor EP2 1 mg/ml IHC Rabbit polyclonal 300


TA200115 Retinoic Acid-Induced 3 (RAI3/RAIG1) 1 mg/ml IHC, ICC Rabbit polyclonal 250

TA200116 Platelet Activating Receptor Homolog (H963) 1 mg/ml IHC Rabbit polyclonal 350

TA200117 Platelet Activating Receptor Homolog (H963) 1 mg/ml IHC Rabbit polyclonal 350

TA200118 Alpha 1c-Adrenoceptor 1 mg/ml IHC Rabbit polyclonal 300


TA200120 Gastric Inhibitory Polypeptide Receptor 1 mg/ml IHC Rabbit polyclonal 250




TA200202 G Protein-Coupled Receptor SLT/MCH2 1 mg/ml IHC, ICC Rabbit polyclonal 200



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TA200203 G Protein-Coupled Receptor SLT/MCH2 1 mg/ml IHC Rabbit polyclonal 300

TA200205 Lysophosphatidic Acid Receptor 1 mg/ml IHC Rabbit polyclonal 350

P2Y9/LPA4/GPR23

TA200207 G Protein-Coupled Receptor SLT/MCH2 1 mg/ml IHC, ICC Rabbit polyclonal 300

TA200124 Neurotensin Receptor Type 2 (NTR2) 1 mg/ml IHC Rabbit polyclonal 300

TA200125 G Protein-Coupled Receptor GPR45 1 mg/ml IHC, ICC Rabbit polyclonal 350

TA200126 Thromboxane A2 Receptor (TBXA2R) 1 mg/ml IHC Rabbit polyclonal 250

TA200127 Thromboxane A2 Receptor (TBXA2R) 1 mg/ml IHC Rabbit polyclonal 250

TA200128 Tachykinin Receptor 2 1 mg/ml IHC, ICC Rabbit polyclonal 350

TA200129 PAEL Receptor (GPR37) 1 mg/ml IHC Rabbit polyclonal 300

TA200130 Tachykinin Receptor 2 1 mg/ml ICC Rabbit polyclonal 250

TA200133 Vasoactive Intestinal Polypeptide Receptor 2 1 mg/ml IHC Rabbit polyclonal 300




TA200135 Proteinase-Activated Receptor 4 1 mg/ml IHC Rabbit polyclonal 200






TA200208 Purinergic Receptor P2Y8 1 mg/ml IHC Rabbit polyclonal 250



TA200141 Cysteinyl Leukotriene Receptor 1 (CysLT1) 1 mg/ml IHC Rabbit polyclonal 300







TA200148 Tachykinin Receptor 1 1 mg/ml IHC Rabbit polyclonal 300

TA200149 CXC Chemokine Receptor 3 (CXCR3) 1 mg/ml IHC Rabbit polyclonal 300

TA200173 G Protein-Coupled Receptor GPR87/GPR95 1 mg/ml IHC Rabbit polyclonal 300






TA200374 Bombesin Receptor Subtype 3 (BRS3) 1 mg/ml IHC Rabbit polyclonal 300

TA200375 Frizzled-9 (FZD9) 1 mg/ml IHC Rabbit polyclonal 350





TA200156 G Protein-Coupled Receptor RDC1 1 mg/ml IHC Rabbit polyclonal 350



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TA200157 Chemokine (C-C motif) Receptor-Like 1 (CCRL1) 1 mg/ml IHC Rabbit polyclonal 300

TA200158 Chemokine (C-C motif) Receptor-Like 1 (CCRL1) 1 mg/ml IHC Rabbit polyclonal 200

TA200159 Thyrotropin (TSH) Receptor 1 mg/ml IHC Rabbit polyclonal 300




TA200163 Latrophilin-3 1 mg/ml IHC Rabbit polyclonal 250

TA200164 G Protein-Coupled Receptor LOC51210 1 mg/ml IHC Rabbit polyclonal 350

TA200168 G Protein-Coupled Receptor SLC/MCH1 1 mg/ml IHC Rabbit polyclonal 200

TA200169 G Protein-Coupled Receptor SLC/MCH1 1 mg/ml IHC Rabbit polyclonal 300

TA200171 Chemokine (C motif) XC Receptor 1 (CCXCR1) 1 mg/ml IHC Rabbit polyclonal 300


TA200165 G Protein-Coupled Receptor LOC51210 1 mg/ml IHC, ICC Rabbit polyclonal 350

TA200166 Mas Proto-Oncogene 1 mg/ml IHC Rabbit polyclonal 350

TA200167 Mas Proto-Oncogene 1 mg/ml IHC Rabbit polyclonal 350

TA200170 P2Y12 Platelet ADP Receptor 1 mg/ml IHC, ICC Rabbit polyclonal 350




TA200179 G Protein-Coupled Receptor G2A 1 mg/ml IHC Rabbit polyclonal 350




TA200183 G Protein-Coupled Receptor GPR86/GPR94/P2Y13 1 mg/ml IHC Rabbit polyclonal 250

TA200184 T-Cell Death-Associated Gene 8 (GPR65) 1 mg/ml IHC Rabbit polyclonal 300

TA200185 T-Cell Death-Associated Gene 8 (GPR65) 1 mg/ml IHC Rabbit polyclonal 300


TA200187 EGF-Like Module-Containing (EMR2) 1 mg/ml IHC, ICC Rabbit polyclonal 250

Mucin-Like Receptor 2

TA200254 Xenotropic And Polytropic 1 mg/ml IHC, ICC Rabbit polyclonal 200

Retrovirus Receptor (XPR1)





TA200192 Protein A 1 mg/ml IHC Rabbit polyclonal 350





TA200197 G Protein-Coupled Receptor GPR63 (PSP24 Beta) 1 mg/ml IHC Rabbit polyclonal 350

TA200198 G Protein-Coupled Receptor SALPR/GPCR135 1 mg/ml IHC Rabbit polyclonal 350

TA200199 G Protein-Coupled Receptor SALPR/GPCR135 1 mg/ml IHC Rabbit polyclonal 350


TA200204 G Protein-Coupled Receptor RDC1 1 mg/ml IHC Rabbit polyclonal 350




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TA200217 Prostate-Specific G Protein-Coupled 1 mg/ml IHC Rabbit polyclonal 350

Receptor (PSGR)

TA200218 Galanin Receptor GalR3 1 mg/ml IHC Rabbit polyclonal 300

TA200219 Galanin Receptor GalR3 1 mg/ml IHC Rabbit polyclonal 300

TA200220 Formyl Peptide Receptor 1 (FPR1) 1 mg/ml IHC Rabbit polyclonal 200

TA200221 Gonadotropin-Releasing Hormone Receptor 1 mg/ml IHC Rabbit polyclonal 300

TA200222 Chemokine Receptor FKSG80/GPR81 1 mg/ml IHC Rabbit polyclonal 350

TA200223 Chemokine Receptor FKSG80/GPR81 1 mg/ml IHC Rabbit polyclonal 350

TA200248 Somatostatin Receptor Type 3 1 mg/ml IHC Rabbit polyclonal 350



TA200253 Xenotropic And Polytropic 1 mg/ml IHC, ICC Rabbit polyclonal 300


TA200231 Melatonin Receptor Type 1A (MT1) 1 mg/ml IHC Rabbit polyclonal 350

TA200232 Melatonin Receptor Type 1A (MT1) 1 mg/ml IHC Rabbit polyclonal 350

TA200235 Follicle-Stimulating Hormone Receptor (FSHR) 1 mg/ml IHC Rabbit polyclonal 200

TA200224 Leukotriene B4 Receptor BLT2 1 mg/ml IHC Rabbit polyclonal 200



TA200227 Lysophosphatidic Acid Receptor Edg2/LPA1 1 mg/ml IHC Rabbit polyclonal 300


TA200229 Encephalopsin 1 mg/ml IHC Rabbit polyclonal 350

TA200230 Encephalopsin 1 mg/ml IHC Rabbit polyclonal 350



TA200236 G Protein-Coupled Receptor TG1019 1 mg/ml IHC Rabbit polyclonal 350

TA200237 Opioid Receptor, Mu 1 (OPRM1) 1 mg/ml IHC Rabbit polyclonal 300

TA200238 Retinoid-Related Orphan Receptor 1 mg/ml IHC Rabbit polyclonal 250

Alpha (ROR Alpha/NR1F1)

TA200239 Opioid Receptor, Mu 1 (OPRM1) 1 mg/ml IHC Rabbit polyclonal 300



TA200062 G Protein-Coupled Receptor TM7XN1/GPR56 1 mg/ml IHC Rabbit polyclonal 350

TA200063 G Protein-Coupled Receptor GPR64 (HE6) 1 mg/ml IHC Rabbit polyclonal 350

TA200064 G Protein-Coupled Receptor GPR64 (HE6) 1 mg/ml IHC Rabbit polyclonal 350

TA200246 Alpha1-Fetoprotein Transcription Factor (NR5A2) 1 mg/ml IHC Rabbit polyclonal 250

TA200247 Xenotropic And Polytropic 1 mg/ml IHC Rabbit polyclonal 200


TA200019 Neuropeptide Y Receptor Type 1 1 mg/ml IHC Rabbit polyclonal 300

TA200021 Prostaglandin E2 Receptor EP3 1 mg/ml IHC Rabbit polyclonal 300

TA200022 Prostaglandin E Receptor EP4 1 mg/ml IHC Rabbit polyclonal 300



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TA200023 Prostaglandin F2-Alpha Receptor 1 mg/ml IHC, ICC Rabbit polyclonal 350

TA200024 Prostaglandin F2-Alpha Receptor 1 mg/ml IHC Rabbit polyclonal 350


TA200026 Sphingolipid Receptor Edg1/S1P1 1 mg/ml IHC Rabbit polyclonal 300


TA200028 Estrogen-Related Receptor 1 mg/ml IHC Rabbit polyclonal 350

Gamma (ERR Gamma/NR3B3)

TA200029 Estrogen-Related Receptor 1 mg/ml IHC Rabbit polyclonal 350

Gamma (ERR Gamma/NR3B3)

TA200030 Formyl Peptide Receptor-Like Receptor 1 (FPRL1) 1 mg/ml IHC Rabbit polyclonal 300

TA200031 GABA(B) Receptor 1 1 mg/ml IHC Rabbit polyclonal 300

TA200032 GABA(B) Receptor 1 1 mg/ml IHC Rabbit polyclonal 300


TA200058 Sphingolipid Receptor Edg3/S1P3 1 mg/ml IHC, ICC Rabbit polyclonal 300


TA200034 Brain-Specific Angiogenesis Inhibitor 3 (BAI3) 1 mg/ml IHC Rabbit polyclonal 350

TA200057 Parathyroid Hormone Receptor 2 (PTHR2) 1 mg/ml IHC Rabbit polyclonal 300

TA200035 Brain-Specific Angiogenesis Inhibitor 3 (BAI3) 1 mg/ml IHC Rabbit polyclonal 350


TA200037 Cannabinoid Receptor 1 (CB1) 1 mg/ml IHC, ICC Rabbit polyclonal 300

TA200038 Cannabinoid Receptor 1 (CB1) 1 mg/ml IHC, ICC Rabbit polyclonal 300

TA200039 Endothelin A Receptor 1 mg/ml IHC Rabbit polyclonal 300

TA200040 Thrombin Receptor 1 mg/ml IHC Rabbit polyclonal 300

TA200041 Formyl Peptide Receptor 1 (FPR1) 1 mg/ml IHC Rabbit polyclonal 300

TA200042 Formyl Peptide Receptor-Like 2 (FPRL2) 1 mg/ml IHC Rabbit polyclonal 350

TA200043 Formyl Peptide Receptor-Like 2 (FPRL2) 1 mg/ml IHC, ICC Rabbit polyclonal 350

TA200044 CXC Chemokine Receptor 4 (CXCR4) 1 mg/ml IHC, ICC Rabbit polyclonal 300


TA200272 Purinergic Receptor P2U2 (GPR91) 1 mg/ml IHC Rabbit polyclonal 350


TA200373 Pyrimidinergic Receptor P2Y4 1 mg/ml IHC Rabbit polyclonal 350



TA200048 Chemokine-Like Receptor 1 (CMKLR1) 1 mg/ml IHC Rabbit polyclonal 350


TA200049 Chemokine-Like Receptor 1 (CMKLR1) 1 mg/ml IHC Rabbit polyclonal 350


TA200052 Purinergic Receptor P2Y6 1 mg/ml IHC, ICC Rabbit polyclonal 350


TA200054 Lysophosphatidic Acid Receptor Edg4/LPA2 1 mg/ml IHC, ICC Rabbit polyclonal 300

TA200055 G Protein-Coupled Receptor GPR44 (CRTH2) 1 mg/ml IHC, ICC Rabbit polyclonal 350




TA200065 Calcitonin Receptor-Like Receptor 1 mg/ml IHC, ICC Rabbit polyclonal 350

TA200265 Prokineticin Receptor 2 (GPR73B) 1 mg/ml IHC Rabbit polyclonal 350



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TA200266 Putative Neurotransmitter Receptor (PNR) 1 mg/ml IHC Rabbit polyclonal 350


TA200066 Calcitonin Receptor-Like Receptor 1 mg/ml IHC, ICC Rabbit polyclonal 350

TA200067 G Protein-Coupled Receptor HM74 1 mg/ml IHC, ICC Rabbit polyclonal 350

TA200068 G Protein-Coupled Receptor HM74 1 mg/ml IHC, ICC Rabbit polyclonal 350

TA200069 G Protein-Coupled Receptor HM74 1 mg/ml IHC Rabbit polyclonal 350


TA200073 G Protein-Coupled Receptor RE2 1 mg/ml IHC, ICC Rabbit polyclonal 350



TA200071 G Protein-Coupled Receptor AGR9 1 mg/ml IHC Rabbit polyclonal 350

TA200072 G Protein-Coupled Receptor AGR9 1 mg/ml IHC Rabbit polyclonal 350



TA200078 Adenosine A1 Receptor 1 mg/ml IHC Rabbit polyclonal 300







TA200086 Melanocortin 1 Receptor (MC1R) 1 mg/ml IHC Rabbit polyclonal 300


TA200088 Prostaglandin F2-Alpha Receptor 1 mg/ml IHC Rabbit polyclonal 250







TA200092 Neuropeptide Y Receptor Type 5 1 mg/ml IHC, ICC Rabbit polyclonal 250



TA200095 Prolactin Releasing Hormone Receptor (GPR10) 1 mg/ml IHC Rabbit polyclonal 250

TA200096 Chemokine (C-C motif) Receptor-like 2 (CCRL2) 1 mg/ml IHC Rabbit polyclonal 350

TA200267 Putative Neurotransmitter Receptor (PNR) 1 mg/ml IHC Rabbit polyclonal 350



TA200097 Chemokine (C-C motif) Receptor-like 2 (CCRL2) 1 mg/ml IHC Rabbit polyclonal 350



TA200103 G Protein-Coupled Receptor GPR2/CCR10 1 mg/ml IHC Rabbit polyclonal 300

TA200104 G Protein-Coupled Receptor GPR2/CCR10 1 mg/ml IHC Rabbit polyclonal 300

TA200107 Histamine H1 Receptor 1 mg/ml IHC Rabbit polyclonal 300


TA200109 Alpha 1d-Adrenoceptor 1 mg/ml IHC Rabbit polyclonal 300



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TA200240 Retinoid-Related Orphan 1 mg/ml IHC Rabbit polyclonal 350

Receptor Beta (ROR Beta/NR1F2)

TA200241 Oxytocin Receptor 1 mg/ml IHC Rabbit polyclonal 300

TA200242 Oxytocin Receptor 1 mg/ml IHC Rabbit polyclonal 300

TA200245 Retinoid-Related Orphan 1 mg/ml IHC Rabbit polyclonal 350

Receptor Beta (ROR Beta/NR1F2)

TA200250 Somatostatin Receptor Type 5 (SSTR5) 1 mg/ml IHC Rabbit polyclonal 300

TA200252 Beta-2 Adrenoceptor 1 mg/ml IHC Rabbit polyclonal 250


TA200259 Vasopressin V2 Receptor 1 mg/ml IHC Rabbit polyclonal 300

TA200260 Vasopressin V2 Receptor 1 mg/ml IHC Rabbit polyclonal 300

TA200261 G Protein-Coupled Receptor MRGX2 1 mg/ml IHC Rabbit polyclonal 350


TA200264 Prokineticin Receptor 1 (GPR73A) 1 mg/ml IHC Rabbit polyclonal 300


TA200273 5-HT1F Receptor 1 mg/ml IHC Rabbit polyclonal 200



TA200276 G Protein-Coupled Receptor GPR85 (SREB2) 1 mg/ml IHC Rabbit polyclonal 350

TA200277 G Protein-Coupled Receptor GPR85 (SREB2) 1 mg/ml IHC Rabbit polyclonal 350

TA200278 G Protein-Coupled Receptor EX33 1 mg/ml IHC Rabbit polyclonal 350

TA200279 G Protein-Coupled Receptor EX33 1 mg/ml IHC Rabbit polyclonal 350

TA200280 Vomeronasal 1 Receptor 1 (VN1R1) 1 mg/ml IHC Rabbit polyclonal 250













TA200293 Bradykinin B1 Receptor 1 mg/ml IHC Rabbit polyclonal 300


TA200295 G Protein-Coupled Receptor D6 1 mg/ml IHC Rabbit polyclonal 300

TA200296 G Protein-Coupled Receptor D6 1 mg/ml IHC Rabbit polyclonal 300



TA200299 G Protein-Coupled Receptor GPR27 (SREB1) 1 mg/ml IHC, ICC Rabbit polyclonal 350

TA200300 Urotensin-II Receptor (GPR14) 1 mg/ml IHC Rabbit polyclonal 300


TA200303 Urotensin-II Receptor (GPR14) 1 mg/ml IHC Rabbit polyclonal 300

TA200304 P2Y12 Platelet ADP Receptor 1 mg/ml IHC, ICC Rabbit polyclonal 350



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TA200305 Neurotensin Receptor Type 2 (NTR2) 1 mg/ml IHC Rabbit polyclonal 350






TA200306 Lysophosphatidic Acid Receptor Edg4/LPA2 1 mg/ml IHC, ICC Rabbit polyclonal 200






TA200318 Olfactory Receptor, Family 2, 1 mg/ml IHC Rabbit polyclonal 350

Subfamily A, Member 4 (OR2A4)




TA200313 Complement Component 5a Receptor 1 1 mg/ml IHC, ICC Rabbit polyclonal 200

TA200316 Prostaglandin E Receptor EP4 1 mg/ml IHC, ICC Rabbit polyclonal 200

TA200317 Prostaglandin E Receptor EP4 1 mg/ml IHC, ICC Rabbit polyclonal 200

TA200319 Prolactin Releasing Hormone Receptor (GPR10) 1 mg/ml IHC Rabbit polyclonal 350

TA200320 Dopamine Receptor D4 (DRD4) 1 mg/ml IHC Rabbit polyclonal 300

TA200324 Neuromedin U Receptor 1 (GPR66) 1 mg/ml IHC Rabbit polyclonal 300

TA200328 Follicle-Stimulating Hormone Receptor (FSHR) 1 mg/ml IHC Rabbit polyclonal 300

TA200329 Endothelin Type B Receptor-Like 1 mg/ml IHC Rabbit polyclonal 350

Protein 2 (ETBR-LP-2)

TA200330 Endothelin Type B Receptor-Like 1 mg/ml IHC Rabbit polyclonal 350

Protein 2 (ETBR-LP-2)


TA200322 Adrenomedullin Receptor L1 1 mg/ml IHC Rabbit polyclonal 250

TA200323 Adrenomedullin Receptor L1 1 mg/ml IHC Rabbit polyclonal 250






TA200327 Melanocortin 2 Receptor (ACTH Receptor) 1 mg/ml IHC Rabbit polyclonal 300




TA200335 G Protein-Coupled Receptor GPRC5B 1 mg/ml IHC Rabbit polyclonal 250









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TA200346 Neuromedin U Receptor 2 1 mg/ml IHC Rabbit polyclonal 350

TA200420 Apelin Receptor (APJ/AGTRL1) 1 mg/ml IHC, ICC Rabbit polyclonal 250









TA200357 Prostacyclin Receptor 1 mg/ml IHC Rabbit polyclonal 350

TA200359 Frizzled-6 (FZD6) 1 mg/ml IHC, ICC Rabbit polyclonal 250

TA200360 Frizzled-6 (FZD6) 1 mg/ml IHC, ICC Rabbit polyclonal 350

TA200361 EBV-Induced Gene 2 1 mg/ml IHC Rabbit polyclonal 350

TA200362 EBV-Induced Gene 2 1 mg/ml IHC Rabbit polyclonal 350

TA200369 Bombesin Receptor Subtype 3 (BRS3) 1 mg/ml IHC Rabbit polyclonal 300



TA200372 G Protein-Coupled Receptor GPR27 (SREB1) 1 mg/ml IHC, ICC Rabbit polyclonal 350


(SNSR1/SNSR2)


(SNSR1/SNSR2)

TA200379 SREB3 1 mg/ml IHC Rabbit polyclonal 350

TA200380 Hepatocyte Nuclear Factor 4 Gamma 1 mg/ml IHC Rabbit polyclonal 300

(HNF4 Gamma/NR2A2)

TA200381 Hepatocyte Nuclear Factor 4 Gamma 1 mg/ml IHC Rabbit polyclonal 300

(HNF4 Gamma/NR2A2)

TA200382 Retinoid-Related Orphan Receptor Gamma 1 mg/ml IHC Rabbit polyclonal 250

(ROR Gamma/NR1F3)


(ROR Gamma/NR1F3)


(ROR Gamma/NR1F3)

TA200412 Apelin Receptor (APJ/AGTRL1) 1 mg/ml IHC Rabbit polyclonal 350

TA200416 5-HT1E Receptor 1 mg/ml IHC Rabbit polyclonal 250



(ROR Gamma/NR1F3)

TA200386 SREB3 1 mg/ml IHC Rabbit polyclonal 350

TA200387 Nuclear Receptor SHP (NR0B2) 1 mg/ml IHC Rabbit polyclonal 250






TA200389 Estrogen-Related Receptor Alpha 1 mg/ml IHC Rabbit polyclonal 350

(ERR Alpha/NR3B1)



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(ERR Alpha/NR3B1)

TA200392 G Protein-Coupled Receptor GPCR150 1 mg/ml IHC Rabbit polyclonal 350


(ERR Alpha/NR3B1)

TA200394 Retinoid-Related Orphan Receptor Beta 1 mg/ml IHC Rabbit polyclonal 250

(ROR Beta/NR1F2)

TA200395 Retinoid-Related Orphan Receptor Alpha 1 mg/ml IHC Rabbit polyclonal 350

(ROR Alpha/NR1F1)

TA200396 ErbA-Related Protein EAR2 (NR2F6) 1 mg/ml IHC Rabbit polyclonal 250

TA200398 G Protein-Coupled Receptor LGR7 1 mg/ml IHC Rabbit polyclonal 300

TA200399 G Protein-Coupled Receptor LGR7 1 mg/ml IHC Rabbit polyclonal 300

TA200405 Photoreceptor-Specific Nuclear Receptor 1 mg/ml IHC Rabbit polyclonal 350

(PNR/NR2E3)

TA200406 Photoreceptor-Specific Nuclear Receptor 1 mg/ml IHC Rabbit polyclonal 350

(PNR/NR2E3)

TA200400 G Protein-Coupled Receptor GPRC5D 1 mg/ml IHC Rabbit polyclonal 350


TA200402 G Protein-Coupled Receptor GPRC5D 1 mg/ml IHC Rabbit polyclonal 350



TA200407 5-HT1B Receptor 1 mg/ml IHC Rabbit polyclonal 200

TA200408 Nuclear Receptor Rev-ErbA Alpha (NR1D1) 1 mg/ml IHC Rabbit polyclonal 250

TA200427 Protein Phosphatase MGC1136 1 mg/ml IHC Rabbit polyclonal 350

TA200428 Protein Phosphatase MGC1136 1 mg/ml IHC Rabbit polyclonal 350


TA200464 Interleukin 8 Receptor B (CXCR2) 1 mg/ml IHC Rabbit polyclonal 200

TA200409 G Protein-Coupled Receptor GPCR150 1 mg/ml IHC Rabbit polyclonal 350


TA200413 EGF-Like Module-Containing Mucin-Like 1 mg/ml IHC, ICC Rabbit polyclonal 250

Receptor 2 (EMR2)

TA200414 EGF-Like Module-Containing Mucin-Like (EMR2) 1 mg/ml IHC, ICC Rabbit polyclonal 350

Receptor 2


TA200434 FLJ20442 1 mg/ml IHC Rabbit polyclonal 300


TA200418 EGF-Like Module-Containing Mucin-Like (EMR2) 1 mg/ml IHC, ICC Rabbit polyclonal 350

Receptor 2

TA200421 5-HT6 Receptor 1 mg/ml IHC Rabbit polyclonal 300

TA200424 Melatonin-Related Receptor 1 mg/ml IHC Rabbit polyclonal 250


TA200429 Protein Tyrosine Phosphatase Alpha (PTPRA) 1 mg/ml IHC Rabbit polyclonal 250

TA200430 Protein Tyrosine Phosphatase Alpha (PTPRA) 1 mg/ml IHC Rabbit polyclonal 250

TA200431 Protein Tyrosine Phosphatase Epsilon (PTPRE) 1 mg/ml IHC Rabbit polyclonal 250



TA200435 MAP Kinase Phosphatase X (MKPX/JSP-1) 1 mg/ml IHC Rabbit polyclonal 250



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TA200440 Alpha 1b-Adrenoceptor 1 mg/ml IHC Rabbit polyclonal 250

TA200443 Estrogen-Related Receptor Beta 1 mg/ml IHC Rabbit polyclonal 350

(ERR Beta/NR3B2)


(ERR Beta/NR3B2)


(ERR Beta/NR3B2)

TA200441 Alpha 1b-Adrenoceptor 1 mg/ml IHC Rabbit polyclonal 300


TA200446 Macrophage-Stimulating 1 Receptor (MST1R/Ron) 1 mg/ml IHC Rabbit polyclonal 350


TA200450 CDC2-Related Protein Kinase 7 (CRK7) 1 mg/ml IHC Rabbit polyclonal 350

TA200480 Lysophosphatidic Acid Receptor 1 mg/ml IHC Rabbit polyclonal 350

P2Y9/LPA4/GPR23

TA200490 Neurotensin Receptor Type 1 1 mg/ml IHC Rabbit polyclonal 300

TA200448 Serum/Glucocorticoid Regulated Kinase-Like 1 mg/ml IHC Rabbit polyclonal 250

(SGKL/SGK3)

TA200449 NIMA (Never In Mitosis Gene A)-Related 1 mg/ml IHC Rabbit polyclonal 250

Kinase 6 (NEK6)


TA200452 Misshapen/NIK-Related Kinase (MINK/MAP4K6) 1 mg/ml IHC Rabbit polyclonal 250




TA200456 A Disintegrin-like and Metalloprotease with 1 mg/ml IHC Rabbit polyclonal 350

Thrombospondin Type 1 Motif, 5 (ADAMTS5)


TA200458 G Protein-Coupled Receptor TM7SF1 1 mg/ml IHC, ICC Rabbit polyclonal 350

TA200459 G Protein-Coupled Receptor TM7SF1 1 mg/ml IHC, ICC Rabbit polyclonal 250

TA200460 G Protein-Coupled Receptor TM7SF1 1 mg/ml IHC Rabbit polyclonal 350


TA200462 Bradykinin B1 Receptor 1 mg/ml IHC Rabbit polyclonal 300

TA200465 Nuclear Receptor Rev-ErbA Alpha (NR1D1) 1 mg/ml IHC Rabbit polyclonal 250


TA200467 Neuromedin B Receptor 1 mg/ml IHC Rabbit polyclonal 300

TA200468 Gastrin-Releasing Peptide Receptor 1 mg/ml IHC Rabbit polyclonal 350

TA200469 Growth Hormone Releasing Hormone Receptor 1 mg/ml IHC Rabbit polyclonal 250










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TA200476 C-C Chemokine Receptor 7 (CCR7) 1 mg/ml IHC, ICC Rabbit polyclonal 200




TA200484 Metabotropic Glutamate Receptor 2 1 mg/ml IHC, W Rabbit polyclonal 200

TA200485 Metabotropic Glutamate Receptor 2 1 mg/ml IHC, ICC, W Rabbit polyclonal 200






TA200493 PACAP Receptor Type 1 1 mg/ml IHC Rabbit polyclonal 350








TA201012 Histone Deacetylase 5 (HDAC5) Antibody 100 µL (10 WB) W IP IHC IC Rabbit H Mk M R Polyclonal 150

TA201013 Alpha-Fodrin Antibody 100 µL (10 WB) W IC Rabbit H Polyclonal 150

TA201092 p21 Waf1/Cip1 (DCS60) Mouse mAb 100 µL (10 WB) W IP IHC Mouse H Mk Monoclonal 150

TA201093 Smac/Diablo Mouse mAb 100 µL (10 WB) W IP IC Mouse H Monoclonal 150

TA201094 Akt2 Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150

TA201095 Akt2 (5B5) Rabbit mAb 100 µL (10 WB) W IP Rabbit H M R Mk Monoclonal 175

TA201096 Akt (5G3) Mouse mAb 100 µL (10 WB) IP IC Mouse H M R Hm Monoclonal 150

TA201097 Akt1 (2H10) Mouse mAb 100 µL (10 WB) W IP IHC F Mouse H M R Monoclonal 150

TA201098 SUV39H1 Histone Methyltransferase Antibody 100 µL (10 WB) W Rabbit H Polyclonal 150

TA201099 p95/NBS1 Antibody 100 µL (10 WB) W IC Rabbit H Mi Mk M R Polyclonal 150

TA201100 NAK Antibody 100 µL (10 WB) W IC Rabbit H M R Polyclonal 150

TA201101 IGF-I Receptor alpha Antibody 100 µL (10 WB) W IP IHC IC Rabbit H M R Polyclonal 150

TA201102 NF-kappaB p65 Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150

TA201103 NF-kappaB p105/p50 Antibody 100 µL (10 WB) W IHC Rabbit H Mk Polyclonal 150

TA201104 MAPKAPK-2 Antibody 100 µL (10 WB) W Rabbit H M R (Hm) Polyclonal 150

TA201105 LKB1 Antibody 100 µL (10 WB) W IC Rabbit H R Polyclonal 150

TA201106 PDK1 Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Polyclonal 150

TA201107 PKR Antibody 100 µL (10 WB) W IP IC Rabbit H M Polyclonal 150

TA201108 C/EBPbeta Antibody 100 µL (10 WB) W Rabbit R Polyclonal 150

TA201109 C/EBPbeta (LAP) Antibody 100 µL (10 WB) W Rabbit H M (R) Polyclonal 150

TA201110 Aurora A/AIK Antibody 100 µL (10 WB) W IC Rabbit H M Mk Polyclonal 150

TA201111 Aurora B/AIM1 Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150

TA201112 Smad2 Antibody 100 µL (10 WB) W IP IC Rabbit H M R Polyclonal 150

TA201113 VASP Antibody 100 µL (10 WB) W IHC Rabbit H Mk Polyclonal 150

TA201114 Met (25H2) Mouse mAb 100 µL (10 WB) W IP Mouse H M R Mk Monoclonal 150



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TA201115 cdk6 (DCS83) Mouse mAb 100 µL (10 WB) W IHC Mouse H M R Monoclonal 150

TA201116 Ezrin/Radixin/Moesin Antibody 100 µL (10 WB) W IHC IC Rabbit W IHC IC Polyclonal 150

TA201117 PDGF Receptor beta Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150

TA201118 Progesterone Receptor (6A1) 100 µL (10 WB) W IP IC Mouse H Monoclonal 150

Monoclonal Antibody

TA201119 CrkL (32H4) Mouse mAb 100 µL (10 WB) W F Mouse H R Hm Monoclonal 150

TA201120 Androgen Receptor Antibody 100 µL (10 WB) W IHC IC Rabbit H Polyclonal 150

TA201121 Etk Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150

TA201122 Gab1 Antibody 100 µL (10 WB) W IHC IP Rabbit H M R Mk Polyclonal 150

TA201123 PAK4 Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Mk Polyclonal 150

TA201124 Blk Antibody 100 µL (10 WB) W Rabbit H Polyclonal 150

TA201125 SGK Antibody 100 µL (10 WB) W IP IC Rabbit H M R Polyclonal 150

TA201126 Pyk2 Antibody 100 µL (10 WB) W IP Rabbit H M Polyclonal 150

TA201127 Cofilin Antibody 100 µL (10 WB) W IP Rabbit H M R Hm Mk Polyclonal 150

TA201128 Ras-GRF1 Antibody 100 µL (10 WB) W IHC Rabbit M Polyclonal 150

TA201129 Jak1 Antibody 100 µL (10 WB) W IP Rabbit H M Polyclonal 150

TA201130 ALK Antibody 100 µL (10 WB) W IP Rabbit H Polyclonal 150

TA201131 Stathmin Antibody 100 µL (10 WB) W IHC Rabbit H Mk M R Polyclonal 150

TA201132 CaMKII Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150

TA201133 Erk5 Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150

TA201134 c-Kit Antibody 100 µL (10 WB) W IP Rabbit H M Polyclonal 150

TA201135 Rad17 Antibody 100 µL (10 WB) W IHC IC Rabbit H Mi Mk M R Polyclonal 150

TA201136 Cytokine Receptor Common beta-Chain Antibody 100 µL (10 WB) W Rabbit H M R Mi Mk Polyclonal 150

TA201137 Phospho-FLT3 (Tyr589/591) (30D4) Rabbit mAb 100 µL (10 WB) W Rabbit M (H) Monoclonal 275

TA201138 FGF Receptor 1 Antibody 100 µL (10 WB) W IP Rabbit H (M) (R) Polyclonal 150

TA201139 CrkII Antibody 100 µL (10 WB) W IC Rabbit H M Polyclonal 150

TA201140 Cortactin Antibody 100 µL (10 WB) W IHC Rabbit H Mk M R Polyclonal 150

TA201141 Connexin 43 Antibody 100 µL (10 WB) W IHC Rabbit H M R Mk Polyclonal 150

TA201142 Btk Antibody 100 µL (10 WB) W Rabbit H M Polyclonal 150

TA201143 NPM Antibody 100 µL (10 WB) W IHC Rabbit H M R Polyclonal 150

TA201144 CD19 Antibody 100 µL (10 WB) W IP IC Rabbit H M Polyclonal 150

TA201145 eIF4B Antibody 100 µL (10 WB) W IHC IC Rabbit H M R Mk Polyclonal 150

TA201146 BLNK Antibody 100 µL (10 WB) W Rabbit H M Polyclonal 150

TA201147 Tuberin Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150

TA201148 Presenilin 1 Antibody 200 µL (10 WB) W IP Rabbit H R Mk (M) Polyclonal 150

TA201149 Nicastrin Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150

TA201150 Myosin Light Chain 2 Antibody 100 µL (10 WB) W Rabbit H M R (C) Polyclonal 150

TA201151 Mst1 Antibody 100 µL (10 WB) W IP IHC Rabbit H Mk M R Polyclonal 150

TA201152 eEF2k Antibody 100 µL (10 WB) W IP IC Rabbit H R Mk Polyclonal 150

TA201153 GRB10 Antibody 100 µL (10 WB) W IP E IHC Rabbit H Hm Polyclonal 150

TA201154 TGF-beta Receptor I Antibody 100 µL (10 WB) W IHC Rabbit H Mi R Polyclonal 150

TA201155 Pin1 Antibody 100 µL (10 WB) W Rabbit H Mk M R (X) Polyclonal 150

TA201156 GP130 Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150

TA201157 SHP-2 Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Polyclonal 150

TA201158 GCK Antibody 100 µL (10 WB) W IP IHC IC Rabbit H M R Mk Polyclonal 150

TA201159 Bak Antibody 100 µL (10 WB) W IP IC Rabbit H Mk Polyclonal 150



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TA201160 LSP1 Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150

TA201161 Mst4 Antibody 100 µL (10 WB) W Rabbit H Mk M R Polyclonal 150

TA201162 PLD1 Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150

TA201163 LIMK1 Antibody 100 µL (10 WB) W IP Rabbit H Mk M R Polyclonal 150

TA201164 LIMK2 Antibody 100 µL (10 WB) W Rabbit H Mk R (M) Polyclonal 150

TA201165 MMP-9 Antibody 100 µL (10 WB) W IHC Rabbit H Polyclonal 150

TA201166 ILK1 Antibody 100 µL (10 WB) W IHC Rabbit H M R Mk Polyclonal 150

TA201167 PLCgamma2 Antibody 100 µL (10 WB) W IP Rabbit H M (R) Polyclonal 150

TA201168 Bcr Antibody 100 µL (10 WB) W F IC Rabbit H M R Polyclonal 150

TA201169 p62Dok Antibody 100 µL (10 WB) W Rabbit H Polyclonal 150

TA201170 p56Dok-2 Antibody 100 µL (10 WB) W Rabbit H M Polyclonal 150

TA201171 IGFBP-2 Antibody 100 µL (10 WB) W IP IHC Rabbit H Polyclonal 150

TA201172 Mst2 Antibody 100 µL (10 WB) W IP Rabbit H Mk M R Polyclonal 150

TA201173 R-Ras Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150

TA201174 Ras Antibody 100 µL (10 WB) W Rabbit H M R Mk (C) (Hm) (X) (Z) Polyclonal 150

TA201175 GRB2 Antibody 100 µL (10 WB) W IHC Rabbit H M R Hm Mk Polyclonal 150

TA201176 GRK2 Antibody 100 µL (10 WB) W IP Rabbit H M R Hm Mk Polyclonal 150

TA201177 G Protein alpha Subunit Antibody 100 µL (10 WB) W IP IHC Rabbit H Mk M R Polyclonal 150

TA201178 Troponin I Antibody 100 µL (10 WB) W Rabbit M (H) (R) Polyclonal 150

TA201179 MMP-2 Antibody 100 µL (10 WB) W Rabbit H Polyclonal 150

TA201180 CaMKIV Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150

TA201181 TCL1 Antibody 100 µL (10 WB) W IHC F Rabbit H (M) Polyclonal 150

TA201182 Akt3 Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150

TA201183 Mst3b Antibody 100 µL (10 WB) W Rabbit H Mk M R Polyclonal 150

TA201184 PDE5 Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150

TA201185 ROR1 Antibody 100 µL (10 WB) W Rabbit M (H) Polyclonal 150

TA201186 ROR2 Antibody 100 µL (10 WB) W IP IC Rabbit M (H) Polyclonal 150

TA201187 Cyclin B1 (V152) Mouse mAb 100 µL (10 WB) W IC F Mouse H M (Hm) Monoclonal 150

TA201188 EGR1 Antibody 100 µL (10 WB) W IP Rabbit R (H) (M) Polyclonal 150

TA201189 MKK7 Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150

TA201190 AMPKbeta1 Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150

TA201191 CDCP1 Antibody 100 µL (10 WB) W Rabbit H Mk Polyclonal 150

TA201192 Tie2 (AB33) Mouse mAb 100 µL (10 WB) W IP Mouse H Monoclonal 150

TA201193 BCL6 Antibody 100 µL (10 WB) W Rabbit H M (R) Polyclonal 150

TA201194 PI3 Kinase p110 gamma Antibody 100 µL (10 WB) W IP Rabbit H M Polyclonal 150

TA201195 PI3 Kinase p110 alpha Antibody 100 µL (10 WB) W IHC Rabbit H M R Polyclonal 150

TA201196 Cytochrome c Antibody 100 µL (10 WB) W IHC Rabbit H M R Mk Polyclonal 150

TA201197 Myt1 Antibody 100 µL (10 WB) W IHC Rabbit H M R (X) Polyclonal 150

TA201198 PI3 Kinase p85 Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150

TA201199 ATP-Citrate Lyase Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150

TA201200 IRAK Antibody 100 µL (10 WB) W Rabbit H Polyclonal 150

TA201201 p44 MAP Kinase (Erk1) Antibody 100 µL (10 WB) W Rabbit M R Polyclonal 150

TA201202 A-Raf Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150

TA201203 Apaf-1 Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150

TA201204 Ran Antibody 100 µL (10 WB) W Rabbit H M R Hm Mk Polyclonal 150

TA201205 HPK1 Antibody 100 µL (10 WB) W IP Rabbit H M Polyclonal 150



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TA201206 PTPmu (BK2) Mouse mAb 100 µL (10 WB) W IP Mouse H R Mi (M) Monoclonal 150

TA201207 Tpl2 Antibody 100 µL (10 WB) W Rabbit R (H) (M) Polyclonal 150

TA201208 IRS-2 Antibody 100 µL (10 WB) W IC Rabbit H Polyclonal 150

TA201209 Bok Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Mk Polyclonal 150

TA201210 Daxx Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150

TA201211 MAP2 Antibody 100 µL (10 WB) W Rabbit H R (M) Polyclonal 150

TA201212 TFII-I Antibody 100 µL (10 WB) W IP IHC Rabbit H M Mk Polyclonal 150

TA201213 Bim Antibody 100 µL (10 WB) W IHC IC Rabbit H M R Mk Polyclonal 150

TA201214 Bik Antibody 100 µL (10 WB) W IHC Rabbit H M R Polyclonal 150

TA201215 DNA-PK Antibody 100 µL (10 WB) W Rabbit H Polyclonal 150

TA201216 CTMP Antibody 100 µL (10 WB) W IP F Rabbit H M R Polyclonal 150

TA201217 A1/Bfl-1 Antibody 100 µL (10 WB) W Rabbit H M Polyclonal 150

TA201218 AIF Antibody 100 µL (10 WB) W IP IHC IC Rabbit H M R Polyclonal 150

TA201219 Cyclin A (BF683) Mouse mAb 100 µL (10 WB) W F Mouse H Monoclonal 150

TA201220 p73 Antibody 100 µL (10 WB) W IC Rabbit H (M) (Mk) Polyclonal 150

TA201221 JNK2 Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150

TA201222 Mad-1 Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150

TA201223 Bmf Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150

TA201224 Integrin beta3 Antibody 100 µL (10 WB) W IP IHC Rabbit H M (R) Polyclonal 150

TA201225 TRAF2 Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150

TA201226 Pim-1 Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150

TA201227 Max Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150

TA201228 RKIP Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150

TA201229 VHR Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150

TA201230 Filamin A Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150

TA201231 PKA C-alpha Antibody 100 µL (10 WB) W IP F Rabbit H M R Polyclonal 150

TA201232 SMC1 Antibody 100 µL (10 WB) W Rabbit H M (R) (C) (X) Polyclonal 150

TA201233 p15 INK4B Antibody 100 µL (10 WB) W F Rabbit H M R Polyclonal 150

TA201234 Cox2 Antibody 100 µL (10 WB) W IP IHC F Rabbit M (H) (R) Polyclonal 150

TA201235 PCTAIRE 1 Antibody 100 µL (10 WB) W IP F Rabbit M R (H) Polyclonal 150

TA201236 HSP70 Antibody 100 µL (10 WB) W IHC Rabbit H M R Mk Polyclonal 150

TA201237 HSP90 Antibody 100 µL (10 WB) W IHC Rabbit H M R Mk (C) (Z) Polyclonal 150

TA201238 p44/42 MAP Kinase Antibody 200 µL (20 WB) W IHC F Rabbit H M R Hm Z Polyclonal 150

TA201239 p42 MAP Kinase (3A7) Mouse mAb 100 µL (10 WB) W IHC F Mouse H M R Hm Monoclonal 150

TA201240 p42 MAP Kinase (Erk2) Antibody 100 µL (10 WB) W IHC Rabbit H M R Mk Polyclonal 150

TA201241 cdc2 Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150

TA201242 cdc2 (POH1) Mouse mAb 100 µL (10 WB) W IP IHC IC Mouse H Mk Monoclonal 150

TA201243 MEK1/2 Antibody 100 µL (10 WB) W F Rabbit H M R Polyclonal 150

TA201244 MEK1 Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150

TA201245 MEK2 Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150

TA201246 Stat3 Antibody 100 µL (10 WB) W IP IHC IC F Rabbit H M R Polyclonal 150

TA201247 Stat3 (124H6) Mouse mAb 100 µL (10 WB) W IP IHC F IC Mouse H M R Mk Monoclonal 150

TA201248 SEK1/MKK4 Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150

TA201249 c-Jun Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150

TA201250 c-Jun (60A8) Rabbit mAb 100 µL (10 WB) W IP IHC Rabbit H M R Mk Monoclonal 175

TA201251 Stat1 Antibody 100 µL (10 WB) W IP F Rabbit H M R Polyclonal 150



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TA201252 Stat1 (9H2) Mouse mAb 100 µL (10 WB) W IP IHC IC Mouse H M Monoclonal 150

TA201253 Elk-1 Antibody 100 µL (10 WB) W IHC Rabbit H M R Polyclonal 150

TA201254 CREB Antibody 100 µL (10 WB) W IP IHC IC Rabbit H M R C Polyclonal 150

TA201255 p70 S6 Kinase Antibody 100 µL (10 WB) W IP IC F Rabbit H M R Mk Polyclonal 150

TA201256 p38 MAP Kinase Antibody 100 µL (10 WB) W IHC F Rabbit H M R Polyclonal 150

TA201257 p38 MAP Kinase delta Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150

TA201258 p38 MAP Kinase (5F11) Mouse mAb 100 µL (10 WB) W IP F Mouse H M R Monoclonal 150

TA201259 p38 MAP Kinase alpha Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Mk Polyclonal 150

TA201260 ATF-2 (20F1) Rabbit mAb 100 µL (10 WB) W IP IHC Rabbit H M R Mk Monoclonal 175

TA201261 MKK3 Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150

TA201262 IkappaB-alpha Antibody 100 µL (10 WB) W IP IHC IC Rabbit H M R Polyclonal 150

TA201263 IkappaB-alpha (112B2) Mouse mAb 100 µL (10 WB) W IP IHC Mouse H R Mk (M) Monoclonal 150

TA201264 IkappaB beta Antibody 100 µL (10 WB) W IHC Rabbit H M R Polyclonal 150

TA201265 IkappaB-epsilon Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150

TA201266 SAPK/JNK Antibody 100 µL (10 WB) W IC F Rabbit H M R Z Polyclonal 150

TA201267 SAPK/JNK (56G8) Rabbit mAb 100 µL (10 WB) W IP Rabbit H M R Mi Mk Monoclonal 175

TA201268 Akt Antibody 100 µL (10 WB) W IP F Rabbit H M R C Hm Polyclonal 150

TA201269 p53 Antibody 100 µL (10 WB) W IP IC Rabbit H R Mk Polyclonal 150

TA201270 Bad Antibody 100 µL (10 WB) W IP IHC IC Rabbit H M R Mk Polyclonal 150

TA201271 Rb (4H1) Mouse mAb 100 µL (10 WB) W IP IHC IC Mouse H R Mk Monoclonal 150

TA201272 GSK-3beta Antibody 100 µL (10 WB) W IHC IC Rabbit H M R (X) (Z) Polyclonal 150

TA201273 RSK1/RSK2/RSK3 Antibody 100 µL (10 WB) W Rabbit H M R Mk (C) Polyclonal 150

TA201274 Stat5 Antibody 100 µL (10 WB) W IP Rabbit H M Polyclonal 150

TA201275 Stat6 Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150

TA201276 c-Myc Antibody 100 µL (10 WB) W IP IC Rabbit H M R Polyclonal 150

TA201277 4E-BP1 Antibody 100 µL (10 WB) W IP IHC IC Rabbit H M R Mk Polyclonal 150

TA201278 FKHR Antibody 100 µL (10 WB) W IHC Rabbit H M R Polyclonal 150

TA201279 AFX Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150

TA201280 Caspase-7 Antibody 100 µL (10 WB) W F Rabbit H M R Polyclonal 150

TA201281 Caspase-9 Antibody (Human Specific) 100 µL (10 WB) W F Rabbit H Polyclonal 150

TA201282 Caspase-9 Antibody (Mouse Specific) 100 µL (10 WB) W IHC Rabbit M Polyclonal 150

TA201283 Caspase-9 Antibody (Rat Specific) 100 µL (10 WB) W IC Rabbit R Polyclonal 150

TA201284 Smad1 Antibody 100 µL (10 WB) W Rabbit H Mk Polyclonal 150

TA201285 Smad4 Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150

TA201286 Smad5 Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150

TA201287 cdc25C Antibody 100 µL (10 WB) W IHC Rabbit H Polyclonal 150

TA201288 cdc25B Antibody 100 µL (10 WB) W IHC Rabbit H Mk M R Polyclonal 150

TA201289 PARP Antibody 100 µL (10 WB) W IHC Rabbit H M R Polyclonal 150

TA201290 PTEN Antibody 100 µL (10 WB) W IP Rabbit H M R Hm Mk Polyclonal 150

TA201291 PTEN (26H9) Mouse mAb 100 µL (10 WB) W IP IHC Mouse H M R Hm Mk Monoclonal 150

TA201292 Beta-Catenin Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Mk Polyclonal 150

TA201293 eNOS Antibody 100 µL (10 WB) W Rabbit H (M) (R) Polyclonal 150

TA201294 Caspase-3 Antibody 100 µL (10 WB) W IP IHC IC F Rabbit H M R Polyclonal 150

TA201295 Caspase-3 (8G10) Rabbit Monoclonal Antibody 100 µL (10 WB) W IP IC Rabbit H M R Monoclonal 175

TA201296 Caspase-3 (3G2 ) Mouse mAb 100 µL (10 WB) W Mouse H Monoclonal 150

TA201297 Pro-TGF-alpha Antibody 100 µL (10 WB) W Rabbit H M (R) Polyclonal 150



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TA201298 eIF2alpha Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150

TA201299 DFF45/DFF35 Antibody 100 µL (10 WB) W IP Rabbit H Polyclonal 150

TA201300 eIF4E Antibody 100 µL (10 WB) W IP Rabbit H M R Z Polyclonal 150

TA201301 Caspase-8 (1C12) Mouse mAb 100 µL (10 WB) W IP F Mouse H Monoclonal 195

TA201302 Caspase-10 Antibody 100 µL (10 WB) W IHC Rabbit H M R Polyclonal 150

TA201303 Caspase-6 Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150

TA201304 MEF2C Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150



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Protein collections

Introduction

The OriGene Protein Collection contains over 100

purified recombinant proteins for scientist to per-

form protein-based applications. The proteins are

mostly associated with transcription, nuclear re-

ceptors and oncology. Functionally active proteins

can be overexpressed in and purified from several

different systems that have been well characterized.

OriGene's manufacturer primarily utilizes bacterial

and baculoviral systems and has produced over 100

transcription and cancer related proteins.

OriGene, through its partnership with an expert

protein producer, is actively preparing functional

proteins every day for our catalog and to address our

customer's bulk protein needs. Customers are

encouraged to email and request that their protein of

interest be placed on the priority list.

On our website, the proteins provided can be

searched by the following criteria,



• Protein Domain

• Keywords

• Gene Family

Product Application

Whereas a human gene transcript supplies the

required information to blueprint the protein

sequence, in order to test cellular function, it is

necessary to produce and test the actual protein.

OriGene has partnered with quality, human protein

providers who derive value from OriGenes large

transcript repository. These high-quality protein

samples are made accessible through this website.

Enzymatic Assays: Protein enzymes can be used to

catalyze reactions of interest in search of substrate

specificity. The researcher can also use such assays

to assess both activation and inhibition of the

enzymes activity. OriGene provides many quality

protein kinases to test their phosphorylation activity.

Protein Binding Studies: Having a properly folded

protein is key to determine its biologically relevant

interactions. This type of study is traditionally

performed through gel-shift assays, ELISA plate

analysis, and various types of affinity chromatography.

Western Blots and SDS-PAGE:The identification of a

proteins rate of migration (Mr) through SDS-PAGE is

best identified through the use of a purified protein.

The proteins available through OriGene are suitable

for this purpose.

Functional Studies: In addition to enzymatic or

binding assays, the researcher can uncover the

cellular function of these proteins through a variety

of specialized assays measuring fluctuations in

second messengers calcium or camp. Transcriptional

regulation can be assessed through off-the-shelf

reporter gene assays.

Quality Control

Functionally active proteins are over expressed in

and purified from several different systems that

have been well characterized. Proteins provided

through OriGene are primarily derived from

bacterial and baculoviral systems to produce

proteins with a focus on transcription and cancer

related proteins. The protein purification strategy

revolves around utilizing protein tags including 6-

histidine, FLAG or myc, to increase purity of the

proteins to near homogeneity upon initial

purification. Initial synthesis involves the creation of

new plasmids containing one or more of the protein

tags that do not interfere with the protein

function(s). This is followed by a second step of

purification (required to further purify recombinant

proteins with a unique protein tag), which will be

decided based on protein structure and property.

These steps involve use of conventional chroma-

tography, fast performance liquid chromatography

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Cat. # Accn Description Unit Source Price*

TP200002 NM_032940 RNA pol II-hRPB5 (RNA polymerase II, 10000 Recombinant human RPB5 is isolated from an E coli 205

p33 subunit) strain that carries the coding sequence of human

RPB5 under the control of a T7 promoter.

TP200003 NM_032940 RNA pol II-hRPB5 (RNA polymerase II, 25000 Recombinant human RPB5 is isolated from an E coli

p33 subunit) strain that carries the coding sequence of human

RPB5 under the control of a T7 promoter. 410

TP200004 X12492 CTF1 CCAAT-Box-Binding Transcription Factor 5000 The wild type human CTF1 protein (499 amino acids) 310

1 (Recombinant Protein from Insect Cells) was expressed in baculovirus system and purified by

using an affinity column and FPLC chromatography.

TP200005 X12492 CTF1 CCAAT-Box-Binding Transcription Factor 12500 The wild type human CTF1 protein (499 amino acids) 620

1 (Recombinant Protein from Insect Cells) was expressed in baculovirus system and purified by


TP200006 NM_000551 VHL von Hippel-Lindau tumor suppressor 5000 The wild type human VHL protein (213 amino acids) 268

protein (Recombinant Protein from Insect Cells) was expressed in baculovirus system and purified

using an affinity column and FPLC chromatography

under non-denaturing condition.

TP200007 NM_000551 VHL von Hippel-Lindau tumor suppressor 12500 The wild type human VHL protein (213 amino acids) 534

protein (Recombinant Protein from Insect Cells) was expressed in baculovirus system and purified

using an affinity column and FPLC chromatography

under non-denaturing condition.

TP200008 NM_001514 TFIIB (Transcription Factor IIB) 10000 Isolated from an E coli strain that carries the coding 205

sequence for human TFIIB under the control of T7

promoter (5).

TP200009 NM_001514 TFIIB (Transcription Factor IIB) 25000 Isolated from an E coli strain that carries the coding 410

sequence for human TFIIB under the control of T7

promoter (5).

TP200010 NM_000800 FGF-1 (acidic fibroblast growth factor) 5000 Recombinant FGF1 was expressed in a baculovirus 237

system and purified by an affinity column in

combination with FPLC chromatography.

TP200011 NM_000800 FGF-1 (acidic fibroblast growth factor) 12500 Recombinant FGF1 was expressed in a baculovirus 473



TP200012 NM_006238 PPAR beta(delta) (Peroxisome proliferator- 10000 Recombinant PPAR is isolated from an E coli strain 204

activated receptor, beta isotype) that carries the coding sequence of the human PPAR

beta under the control of a T7 promoter.

TP200013 NM_006238 PPAR beta(delta) (Peroxisome proliferator- 25000 Recombinant PPAR is isolated from an E coli strain 407

activated receptor, beta isotype) that carries the coding sequence of the human PPAR

beta under the control of a T7 promoter.

TP200014 NM_005225 E2F-1, (RBAP-1) Transcription Factor 5000 The wild type E2F-1 (residue 1-437) was expressed in 310

and Rb-mediator baculovirus system and purified in combination of an

affinity column and FPLC chromatography.

TP200015 NM_005225 E2F-1, (RBAP-1) Transcription Factor 12500 The wild type E2F-1 (residue 1-437) was expressed in 620

and Rb-mediator baculovirus system and purified in combination of an


(FPLC) and / or high-pressure liquid chromatography

(HPLC) based techniques to achieve highly purified

proteins. Quality is monitored by SDS-PAGE.

Proteins are purified greater than 95% homogeneous

and contain no detectable protease, DNase, and

RNase activity.

Product offering is subject to change. See our website for the most updated information.

Product Listing

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TP200016 NM_002467 c-Myc (proto oncogene) 5000 Recombinant c-myc was expressed in a bacterial 258


combination with FPLC chromatography

TP200017 NM_002467 c-Myc (proto oncogene) 12500 Recombinant c-myc was expressed in a bacterial


combination with FPLC chromatography 515

TP200018 NM_021976 RXR beta (Retinoid X Receptor, beta isoform) 5000 Recombinant RXR beta is expressed in baculovirus 237



TP200019 NM_021976 RXR beta (Retinoid X Receptor, beta isoform) 12500 Recombinant RXR beta is expressed in baculovirus 473



TP200020 NM_021144 p52, Transcriptional Coactivator 10000 Recombinant p52 protein (wild type, 333 amino 258

acids) is isolated from an E coli strain that carries

the coding sequence of human p52 under the control

of T7 promoter and purified by the combination of

an affinity column and FPLC chromatography.

TP200021 NM_021144 p52, Transcriptional Coactivator 25000 Recombinant p52 protein (wild type, 333 amino 515

acids) is isolated from an E coli strain that carries


of T7 promoter and purified by the combination

of an affinity column and FPLC chromatography.

TP200022 NM_015859 TFIIA-p55 (Transription Factor IIA, p55 subunit) 10000 p55 subunit of TFIIA is isolated form a strain of 205

E coli that contains the coding sequence of human

p55 of TFIIA under the control of T7 promoter(3).

TP200023 NM_015859 TFIIA-p55 (Transription Factor IIA, p55 subunit) 25000 p55 subunit of TFIIA is isolated form a strain of 410

E coli that contains the coding sequence of human

p55 of TFIIA under the control of T7 promoter(3).

TP200024 NM_004492 TFIIA (Transription Factor IIA, reconstituted) 10000 p55 subunit of TFIIA is isolated form a strain of E coli 205

that contains the coding sequence of human

p55 of TFIIA under the control of T7 promoter (3).

p12 subunit of TFIIA is isolated from a strain of E coli

that contains the coding sequence for human

TFIIA g subunit under the control of T7 promoter.

TFIIA was reconstituted as described in (5).

TP200025 NM_004492 TFIIA (Transription Factor IIA, reconstituted) 25000 p55 subunit of TFIIA is isolated form a strain of E coli 410

that contains the coding sequence of human

p55 of TFIIA under the control of T7 promoter (3).

p12 subunit of TFIIA is isolated from a strain of E coli

that contains the coding sequence for human

TFIIA g subunit under the control of T7 promoter.

TFIIA was reconstituted as described in (5).

TP200026 NM_007296 BRCA1, Tumor Suppressor Protein 2000 The wild type BRCA1 (1-1863 residues) is expressed in 310

and Transcription Factor baculovirus system and purified by an affinity column

in combination with FPLC chromatography.

TP200027 NM_007296 BRCA1, Tumor Suppressor Protein 5000 The wild type BRCA1 (1-1863 residues) is expressed in 620



TP200028 NM_007121 LXR- beta (Liver-X Receptor, beta isoform) 10000 Recombinant LXR is isolated from an E coli strain that 204

carries the coding sequence of the human LXR beta

under the control of a T7 promoter.

TP200029 NM_007121 LXR- beta (Liver-X Receptor, beta isoform) 25000 Recombinant LXR is isolated from an E coli strain that 407

carries the coding sequence of the human LXR beta


TP200030 NM_006924 ASF/SF2, (SFRS1 or SRp30a from E Coli) 10000 The human ASF/SF2 wild type protein (residue1-248) 215

Pre-mRNA Splicing Factor was expressed in E Coli and purified by an affinity

column.


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TP200031 NM_006924 ASF/SF2, (SFRS1 or SRp30a from E Coli) 25000 The human ASF/SF2 wild type protein (residue1-248) 429

Pre-mRNA Splicing Factor was expressed in E Coli and purified by an affinity

column.

TP200032 NM_006924 ASF/SF2, (SFRS1 or SRp30a from Baculovirus) 5000 The human ASF/SF2 wild type protein (residue1-248) 310

Pre-mRNA Splicing Factor was expressed in baculovirus system and purified by

an affinity column in combination with FPLC

chromatography.

TP200033 NM_006924 ASF/SF2, (SFRS1 or SRp30a from Baculovirus) 12500 The human ASF/SF2 wild type protein (residue1-248) 620

Pre-mRNA Splicing Factor was expressed in baculovirus system and purified by

an affinity column in combination with FPLC

chromatography.

TP200034 NM_006713 PC4 (Positive Cofactor 4, wild type) 10000 Recombinant PC4 protein (wild type, 127 amino 205

Transcriptional Coactivator acids) is isolated from an E coli strain that carries the

coding sequence of human PC4 under the control of

T7 promoter and purified by conventional

chromatography.

TP200035 NM_006713 PC4 (Positive Cofactor 4, wild type) 25000 Recombinant PC4 protein (wild type, 127 amino 410




chromatography.

TP200036 NM_006713 PC4-mt (Positive Cofactor 4, F77P mutant) 10000 Recombinant PC4 protein (mutant F77P, 127 amino 205




chromatography.

TP200037 NM_006713 PC4-mt (Positive Cofactor 4, F77P mutant) 25000 Recombinant PC4 protein (mutant F77P, 127 amino 410




chromatography.

TP200038 NM_006713 PC4-mt (Positive Cofactor 4, serine mutations) 10000 Recombinant PC4 protein (serine mutations, 127 205

Transcriptional Coactivator amino acids) is isolated from an E coli strain that

carries the coding sequence of human PC4 under the

control of T7 promoter and purified by conventional

chromatography.

TP200039 NM_006713 PC4-mt (Positive Cofactor 4, serine mutations) 25000 Recombinant PC4 protein (serine mutations, 127 410

Transcriptional Coactivator amino acids) is isolated from an E coli strain that

carries the coding sequence of human PC4 under the

control of T7 promoter and purified by conventional

chromatography.

TP200040 NM_006233 RNA pol II-hRPB9 (RNA polymerase II, 10000 Recombinant p14.5 is isolated from an E coli strain 205

p14.5 subunit) that carries the coding sequence of human RPB9


TP200041 NM_006233 RNA pol II-hRPB9 (RNA polymerase II, 25000 Recombinant p14.5 is isolated from an E coli strain 410

p14.5 subunit) that carries the coding sequence of human RPB9


TP200042 NM_005693 LXR- alpha (Liver-X Receptor, alpha isoform) 10000 Recombinant LXR is isolated from an E coli strain 204

that carries the coding sequence of the human

LXR alpha under the control of a T7 promoter.

TP200043 NM_005693 LXR- alpha (Liver-X Receptor, alpha isoform) 25000 Recombinant LXR is isolated from an E coli strain 407

that carries the coding sequence of the human LXR

alpha under the control of a T7 promoter.

TP200044 NM_005693 LXR-LBD (Liver-X Receptor, ligand binding 10000 Recombinant LXR-LBD is isolated from an E coli 195

domain) strain that carries the coding sequence of the human

LXR under the control of a T7 promoter.

TP200045 NM_005693 LXR-LBD (Liver-X Receptor, ligand binding 25000 Recombinant LXR-LBD is isolated from an E coli 389

domain) strain that carries the coding sequence of the human

LXR under the control of a T7 promoter.


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TP200046 NM_005513 TFIIE alpha, p56 (Transcription Factor IIE, 10000 The recombinant protein is purified from an E coli 205

alpha subunit) strain that contains the coding sequence of human

TFIIE a subunit under the control of T7

promoter (5).

TP200047 NM_005513 TFIIE alpha, p56 (Transcription Factor IIE, 25000 The recombinant protein is purified from an E coli 410

alpha subunit) strain that contains the coding sequence of human

TFIIE a subunit under the control of T7

promoter (5).

TP200048 NM_002095 TFIIE (Transcription Factor IIE, alpha+ 10000 The recombinant proteins are purified from an E coli 205

beta subunits) strain that contains the coding sequence of human

TFIIE a or b subunit under the control of T7 promoter

(5) (cat. #P1005 and #P1006, respectively) and

functional TFIIE is reconstituted as a heterotetramer

by combining both subunits in a 1:1 molar ratio.

TP200049 NM_002095 TFIIE (Transcription Factor IIE, alpha+ 25000 The recombinant proteins are purified from an E coli 410

beta subunits) strain that contains the coding sequence of human

TFIIE a or b subunit under the control of T7 promoter

(5) (cat. #P1005 and #P1006, respectively) and

functional TFIIE is reconstituted as a heterotetramer

by combining both subunits in a 1:1 molar ratio.

TP200050 NM_005316 TFIIH-p62 (Transcription Factor IIH, p62 10000 Recombinant p62 protein is isolated from an E coli 205

subunit) strain that carries the coding sequence of human p62


TP200051 NM_005316 TFIIH-p62 (Transcription Factor IIH, p62 25000 Recombinant p62 protein is isolated from an E coli 410

subunit) strain that carries the coding sequence of human p62


TP200052 NM_005036 PPAR alpha (Peroxisome proliferator- 10000 Recombinant PPAR is isolated from an E coli strain 204

activated receptor, alpha isotype) that carries the coding sequence of the human PPAR


TP200053 NM_005036 PPAR alpha (Peroxisome proliferator- 25000 Recombinant PPAR is isolated from an E coli strain 407

activated receptor, alpha isotype) that carries the coding sequence of the human PPAR


TP200054 NM_004492 TFIIA-p12 (Transcription Factor IIA, subunit) 10000 p12 subunit of TFIIA is isolated from a strain of E coli 205

that contains the coding sequence for human TFIIA

gamma subunit under the control of T7 promoter.

TP200055 NM_004492 TFIIA-p12 (Transcription Factor IIA, subunit) 25000 p12 subunit of TFIIA is isolated from a strain of E coli 410

that contains the coding sequence for human TFIIA

gamma subunit under the control of T7 promoter.

TP200056 NM_003921 Bcl-10 (B Cell Lymphoma 10) 6000 The wild type human Bcl-10 protein was expressed 268

in baculovirus system and purified by an affinity

column in combination with FPLC chromatography.

TP200057 NM_003921 Bcl-10 (B Cell Lymphoma 10) 15000 The wild type human Bcl-10 protein was expressed in 534

baculovirus system and purified by an affinity column


TP200058 NM_003250 TR- alpha1 (Thyroid Hormone Receptor, 5000 The wild type human TR alpha 1 was expressed in 237

alpha1 isoform) baculovirus system and purified by an affinity column


TP200059 NM_003250 TR- alpha1 (Thyroid Hormone Receptor, 12500 The wild type human TR alpha 1 was expressed in 473

alpha1 isoform) baculovirus system and purified by an affinity column


TP200060 NM_003286 Topo I Human DNA Topoisomerase I (wild type) 5000 The wild type DNA topoisomerase I protein (765 258

amino acids) was expressed in baculovirus system

and purified by using an affinity column and FPLC

chromatography.

TP200061 NM_003286 Topo I Human DNA Topoisomerase I (wild type) 12500 The wild type DNA topoisomerase I protein (765 515

amino acids) was expressed in baculovirus system

and purified by using an affinity column and FPLC

chromatography.


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TP200062 NM_003286 Topo I (C651-765) Human DNA Topoisomerase I 5000 The C-terminal domain of DNA topoisomerase I 258

(C terminal domain) protein (residues 651-765) was expressed in

baculovirus system and purified by using an affinity

column and FPLC chromatography.

TP200063 NM_003286 Topo I (C651-765) Human DNA Topoisomerase I 12500 The C-terminal domain of DNA topoisomerase I 515

(C terminal domain) protein (residues 651-765) was expressed in

baculovirus system and purified by using an affinity

column and FPLC chromatography.

TP200064 NM_003286 Topo I (197-651) Human DNA Topoisomerase I 5000 The core domain of DNA topoisomerase I protein 258

(Core Domain) (residues 197-651) was expressed in baculovirus

system and purified by using an affinity column and

FPLC chromatography.

TP200065 NM_003286 Topo I (197-651) Human DNA Topoisomerase I 12500 The core domain of DNA topoisomerase I protein 515

(Core Domain) (residues 197-651) was expressed in baculovirus

system and purified by using an affinity column and

FPLC chromatography.

TP200066 NM_003286 Topo I (N1-197) Human DNA Topoisomerase I 5000 The N-terminal domain (NTD) of human DNA 258

(NTD) topoisomerase I protein (residue 1-197) was expressed

in baculovirus system and purified by using an


TP200067 NM_003286 Topo I (N1-197) Human DNA Topoisomerase I 12500 The N-terminal domain (NTD) of human DNA 515

(NTD) topoisomerase I protein (residue 1-197) was expressed

in baculovirus system and purified by using an


TP200068 NM_003286 Topo I (Y723F) Human DNA Topoisomerase I 5000 The mutant Y723F of DNA topoisomerase I protein 258

(mt Y723F) (Y723F) was expressed in baculovirus system and

purified by using an affinity column and FPLC

chromatography.

TP200069 NM_003286 Topo I (Y723F) Human DNA Topoisomerase I 12500 The mutant Y723F of DNA topoisomerase I protein 515

(mt Y723F) (Y723F) was expressed in baculovirus system and

purified by using an affinity column and FPLC

chromatography.

TP200070 NM_003194 TBP (TATA box Binding Protein) 10000 Isolated from an E coli strain that carries the coding 205

sequence for human TBP under the control off T7

promoter (3).

TP200071 NM_003194 TBP (TATA box Binding Protein) 25000 Isolated from an E coli strain that carries the coding 410

sequence for human TBP under the control off T7

promoter (3).

TP200072 NM_003131 c-Fos (proto oncogene) 5000 Recombinant c-fos was expressed in a baculovirus 258



TP200073 NM_003131 c-Fos (proto oncogene) 12500 Recombinant c-fos was expressed in a baculovirus 515



TP200074 NM_003072 BRG1 (Brahma-related Gene 1 Protein, 5000 The wild type human BRG1 was expressed in 310

wild type) baculovirus system and purified by an affinity

column in combination with FPLC chromatography.

TP200075 NM_003072 BRG1 (Brahma-related Gene 1 Protein, 12500 The wild type human BRG1 was expressed in 620

wild type) baculovirus system and purified by an affinity column


TP200076 NM_003072 BRG1-mt (Brahma-related Gene 1 Protein, 5000 The mutant human BRG1 (K798R) was expressed in 258

K798R) baculovirus system and purified by an affinity column


TP200077 NM_003072 BRG1-mt (Brahma-related Gene 1 Protein, 12500 The mutant human BRG1 (K798R) was expressed in 515

K798R) baculovirus system and purified by an affinity column


TP200078 NM_003016 SC35, (SFRS2 or SRp30b) Pre-mRNA 5000 The human SC35 wild type protein was expressed in 258

Splicing Factor baculovirus system and purified by an affinity column



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TP200079 NM_003016 SC35, (SFRS2 or SRp30b) Pre-mRNA 12500 The human SC35 wild type protein was expressed in 515

Splicing Factor baculovirus system and purified by an affinity column


TP200080 NM_002957 RXR alpha (Retinoid X receptor protein) 10000 Recombinant RXR is isolated from an E coli strain 205


protein under the control of a T7 promoter.

TP200081 NM_002957 RXR alpha (Retinoid X receptor protein) 25000 Recombinant RXR is isolated from an E coli strain 410



TP200082 NM_002957 RXR-LBD (RXR alpha 198-462) 10000 Recombinant RXR-LBD is isolated from an E coli strain 195

that carries the coding sequence of the human RXR-

LBD under the control of a T7 promoter.

TP200083 NM_002957 RXR-LBD (RXR alpha 198-462) 25000 Recombinant RXR-LBD is isolated from an E coli strain 389

that carries the coding sequence of the human RXR-

LBD under the control of a T7 promoter.

TP200084 NM_002875 RAD51 10000 Recombinant RAD51 was expressed in a strain of 205

E coli and purified by an affinity column in


TP200085 NM_002875 RAD51 25000 Recombinant RAD51 was expressed in a strain of 410

E coli and purified by an affinity column in


TP200086 NM_002228 c-Jun (proto oncogene) 5000 Recombinant c-jun was expressed in a baculovirus 258



TP200087 NM_002228 c-Jun (proto oncogene) 12500 Recombinant c-jun was expressed in a baculovirus 515



TP200088 NM_002128 HMG1 (High Mobility Group 1 protein) 10000 Recombinant HMG1 is isolated from an E coli strain 195

that carries the coding sequence of the human HMG1


TP200089 NM_002128 HMG1 (High Mobility Group 1 protein) 25000 Recombinant HMG1 is isolated from an E coli strain 389

that carries the coding sequence of the human HMG1


TP200090 NM_002128 HMG1 (Native from Calf Thymus) 10000 HMG1 was purified from calf thymus using several 258

steps of conventional and FPLC chromatography.

TP200091 NM_002128 HMG1 (Native from Calf Thymus) 25000 HMG1 was purified from calf thymus using several 515

steps of conventional and FPLC chromatography.

TP200092 NM_002095 TFIIE beta, p34 (Transcription Factor IIE, 10000 The recombinant protein is purified from an E coli 205

beta subunit) strain that contains the coding sequence of human

TFIIE subunit under the control of T7 promoter (4).

TP200093 NM_002095 TFIIE beta, p34 (Transcription Factor IIE, 25000 The recombinant protein is purified from an E coli 410

beta subunit) strain that contains the coding sequence of human

TFIIE subunit under the control of T7 promoter (4).

TP200094 NM_002096 TFIIF, RAP74 (Transcription Factor IIF, 10000 Recombinant RAP74 protein is isolated from an E coli 205

Rap74 subunit) strain that carries the coding sequence of human

RAP74, the subunit of TFIIF under the control of T7

promoter.

TP200095 NM_002096 TFIIF, RAP74 (Transcription Factor IIF, 25000 Recombinant RAP74 protein is isolated from an E coli 410

Rap74 subunit) strain that carries the coding sequence of human

RAP74, the subunit of TFIIF under the control of T7

promoter.

TP200096 NM_002006 FGF-2 (basic fibroblast growth factor) 5000 Recombinant FGF2 was expressed in a baculovirus 237



TP200097 NM_002006 FGF-2 (basic fibroblast growth factor) 12500 Recombinant FGF2 was expressed in a baculovirus 473




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TP200098 NM_001938 Dr1 (NC2 beta 19 kDa) 10000 Recombinant Dr1 is isolated from an E coli strain 195


Dr1 under the control of a T7 promoter.

TP200099 NM_001938 Dr1 (NC2 beta 19 kDa) 25000 Recombinant Dr1 is isolated from an E coli strain 389


Dr1 under the control of a T7 promoter.

TP200100 NM_001429 p300 Tumor Suppressor Protein and 4000 The wild type p300 (2414 amino acid residues) was 310

Transcription Factor expressed in baculovirus system and purified by an

affinity column in combination with FPLC

chromatography.

TP200101 NM_001429 p300 Tumor Suppressor Protein and 10000 The wild type p300 (2414 amino acid residues) was 620

Transcription Factor expressed in baculovirus system and purified by an


chromatography.

TP200102 NM_001429 p300 (C1135-2414) p300 C-terminus 4000 The C-terminus of p300 (residues 1135-2414) was 310

expressed in baculovirus system and purified by an


chromatography.

TP200103 NM_001429 p300 (C1135-2414) p300 C-terminus 10000 The C-terminus of p300 (residues 1135-2414) was 620

expressed in baculovirus system and purified by an


chromatography.

TP200104 NM_000964 RAR- alpha (Retinoic Acid Receptor, 5000 Recombinant RAR alpha was expressed in a 270

alpha isoform) baculovirus system and purified by an affinity column

in combination with FPLC chromatography

TP200105 NM_000964 RAR- alpha (Retinoic Acid Receptor, 12500 Recombinant RAR alpha was expressed in a 539

alpha isoform) baculovirus system and purified by an affinity column


TP200106 NM_000965 RAR- gamma (Retinoic Acid Receptor, 5000 Recombinant RAR gamma was expressed in a 258

gamma isoform) baculovirus system and purified by an affinity column


TP200107 NM_000965 RAR- gamma (Retinoic Acid Receptor, 12500 Recombinant RAR gamma was expressed in a 539

gamma isoform) baculovirus system and purified by an affinity column


TP200108 NM_000966 RAR- beta (Retinoic Acid Receptor, 5000 Recombinant RAR beta was expressed in a 270

beta isoform) baculovirus system and purified by an affinity column


TP200109 NM_000966 RAR- beta (Retinoic Acid Receptor, 12500 Recombinant RAR beta was expressed in a 539

beta isoform) baculovirus system and purified by an affinity column


TP200110 NM_033222 p75, Transcriptional Coactivator and Lens 10000 Recombinant p75 protein (wild type, 530 amino 258

Epithelial Cell-Derived Growth Factor acids) is isolated from an E coli strain that carries


of T7 promoter and purified by an affinity column in


TP200111 NM_033222 p75, Transcriptional Coactivator and Lens 25000 Recombinant p75 protein (wild type, 530 amino 515

Epithelial Cell-Derived Growth Factor acids) is isolated from an E coli strain that carries the

coding sequence of human p75 under the control of

T7 promoter and purified by an affinity column in


TP200112 NM_033222 p75-CTR [C-terminal region of p75 (LEDGF)] 10000 Recombinant p75 CTR is isolated from an E coli strain 258

that carries the C-terminal coding sequence of the

human LEDGF/p75 (amino acid 322-530) under the

control of a T7 promoter.

TP200113 NM_033222 p75-CTR [C-terminal region of p75 (LEDGF)] 25000 Recombinant p75 CTR is isolated from an E coli strain 515

that carries the C-terminal coding sequence of the

human LEDGF/p75 (amino acid 322-530) under the

control of a T7 promoter.


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TP200114 NM_000546 p53 (wild type) Tumor Suppressor Protein 5000 The wild type p53 (393 amino acids was expressed in 275



TP200115 NM_000546 p53 (wild type) Tumor Suppressor Protein 12500 The wild type p53 (393 amino acids was expressed in 550



TP200116 NM_000546 p53 (1-363, C-terminal deletion) Tumor 5000 The C-terminus-deleted p53 (amino acid 1-363) was 275

Suppressor Protein and Transcription Factor expressed in baculovirus system and purified by an


chromatography.




chromatography.




chromatography.




chromatography.

TP200120 NM_000461 TR beta1 (Thyroid Hormone Receptor, 10000 Recombinant TR is isolated from an E coli strain that 205

beta1 isoform) carries the coding sequence of the human TR

alpha1 isoform under the control of a T7 promoter.

TP200121 NM_000461 TR beta1 (Thyroid Hormone Receptor, 25000 Recombinant TR is isolated from an E coli strain that 410

beta1 isoform) carries the coding sequence of the human TR

alpha1 isoform under the control of a T7 promoter.

TP200122 NM_000321 pRB (Retinoblastoma protein), Tumor 5000 The wild type pRB (residue1-928) is expressed in 310

Suppressor Protein, Transcription Factor baculovirus system and purified by an affinity column

and Proliferation Facto in combination with FPLC chromatography.

TP200123 NM_000321 pRB (Retinoblastoma protein), Tumor 12500 The wild type pRB (residue1-928) is expressed in 620

Suppressor Protein, Transcription Factor baculovirus system and purified by an affinity column

and Proliferation Facto in combination with FPLC chromatography.

TP200124 NM_000176 GR (Glucocorticoid Receptor) 5000 Recombinant GR was expressed in a baculovirus 258



TP200125 NM_000176 GR (Glucocorticoid Receptor) 12500 Recombinant GR was expressed in a baculovirus 515



TP200126 NM_000125 ER (Estrogen Receptor) 4000 Recombinant ER was expressed in a baculovirus 325



TP200127 NM_000125 ER (Estrogen Receptor) 10000 Recombinant ER was expressed in a baculovirus 649



TP200128 NM_138473 GAL4-Sp1<sup>Q</sup> [GAL4(94) + 4000 Recombinant GAL4-Sp1 is isolated from an E coli 258

Sp1(266-516)] strain that carries the coding sequence of the fused


TP200129 NM_138473 GAL4-Sp1<sup>Q</sup> [GAL4(94) + 10000 Recombinant GAL4-Sp1 is isolated from an E coli 515

Sp1(266-516)] strain that carries the coding sequence of the fused


TP200130 NM_138473 Sp1 GC-box Binding Protein (Natural 2000 Natural Sp1 fraction was purified from HeLa cell 325

fraction purified from HeLa Cells) nuclear extract by using wheat germ agglutinin

affinity chromatography (12).

TP200131 NM_138473 Sp1 GC-box Binding Protein (Natural 5000 Natural Sp1 fraction was purified from HeLa cell 649

fraction purified from HeLa Cells) nuclear extract by using wheat germ agglutinin

affinity chromatography (12).


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TP200132 NM_138473 Sp1 GC-box Binding Protein (Recombinant 5000 The wild type Sp1 protein (785 amino acids) was 310

protein purified from insect cells) expressed in baculovirus system and purified by


TP200133 NM_138473 Sp1 GC-box Binding Protein (Recombinant 12500 The wild type Sp1 protein (785 amino acids) was 620

protein purified from insect cells) expressed in baculovirus system and purified by


TP200134 NM_138712 PPAR gamma (Peroxisome proliferator- 10000 Recombinant PPAR is isolated from an E coli strain 204

activated receptor, gamma isotype) that carries the coding sequence of the human

PPAR gamma under the control of a T7 promoter.

TP200135 NM_138712 PPAR gamma (Peroxisome proliferator- 25000 Recombinant PPAR is isolated from an E coli strain 407

activated receptor, gamma isotype) that carries the coding sequence of the human

PPAR gamma under the control of a T7 promoter.


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Quanti-Ladder™ DNA Marker

The Quanti-Ladder™ DNA Marker is a ready-to-use

molecular weight marker, ideal for easy quan-

tification and molecular weight determination. The

size standards range from 200 to 10,000 base pairs of

double-stranded DNA.

Product Description

Quanti-Ladder™ DNA Marker consists of bands of

0.2, 0.4, 0.6, 0.8, 1.0, 1.5, 2.0, 2.5, 3.0, 4.0, 5.0, 6.0, 8.0

and 10 kilobases. The 1.0 and 10 kilobase-pairs bands

have a higher intensity than the others to allow quick

and easy identification. Using a standard loading of

5 µL, each band corresponds to an exact quantitation

of DNA. This allows for easy quantification of any

DNA sample.

Quanti-Ladder™ DNA Marker is prepared in a load-

ing buffer containing the tracking dyes bromophenol

blue and xylene cyanol FF and is available in two

formats:

• 1 mL or 200 lanes equivalent

• 5 mL or 1000 lanes equivalent

SIZE (kbp) ng/band

10 100

8.0 80

6.0 60

5.0 50

4.0 40

3.0 30

2.5 25

2.0 20

1.5 15

1.0 100

0.8 80

0.6 60

0.4 40

0.2 20


Quanti-Ladder™ DNA Markers, 200 lanes QLD-200 $150

Quanti-Ladder™ DNA Markers, 1000 lanes QLD-1000 $595

ADVANTAGES

Ideal for easy quantificationof DNA samples

14 DNA bands for accuratesize determination

Easy to remember sizesranging from 0.2 to 10 kb

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Millennium™ RNA Marker

The Millennium™ RNA Marker has been developed

to provide easy and accurate molecular weight

determination of single-stranded RNAs from 0.5 to

9.0 kb, and is ideal for Northern blot protocols.

Product Description

The RNA ladder consists of a series of transcripts of

0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 4.0, 5.0, 6.0 and 9.0

kilobases. This large number of well-separated RNA

bands allows the construction of a very accurate

standard curve for determining the sizes of unknown

mRNA species. Each lot contains bands at

approximately the same intensity and is free of

nuclease contamination.

The Millennium™ RNA Marker is provided at a

concentration of 1 µg/µL. The bands are easily

viewed by ethidium bromide staining when 2 µL are

separated in a denaturing agarose gel.

ADVANTAGES

Ten RNA bands foraccurate size determination

Easy-to-remember sizesranging from 0.5 to 9.0 kb

Markers hybridize with λ DNA forautoradiographic detection


Millennium™ RNA Markers, 25 lanes RMM-3703 $105

9

6

5

4

3

2.5

1.5

0.5

1

2

size (kb)

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Rapid-Load™ PCR Loading Dye

Rapid-Load™ is a 5x sample loading dye that is

added to a PCR mix before amplification. The

presence of Rapid-Load™ in the PCR mix will not

interfere with the performance of the overall reaction.

After the PCR is finished, the reaction products can be

directly loaded in an agarose gel. It is not necessary

to add standard loading dye to the samples. It

eliminates the need for an extra pipetting step.

Product Description

Rapid-Load™ is red in color making it easy to load

samples and track them in an agarose gel during

electrophoresis. The red dye migrates at 1.4 kbp in a

1% agarose gel with 1x TAE running buffer.

Rapid-Load™ can be used in PCR amplifications

containing standard plasmid DNA, genomic DNA or

bacterial colonies. It is also ideal for use with

multiple samples such as when performing a 96-well

PCR.

Rapid-Load™ is available in two formats:

• 5 mL or 500 PCR equivalent

• 25 mL or 2500 PCR equivalent

Fig. 1 shows PCR amplification of human b-actin from

plasmid DNA, transformed bacterial colonies or geno-

mic DNA. Each reaction was performed in duplicate in

the presence (+) or absence (-) of Rapid-Load™.

– +– +– +

plasmidDNA

bacterialcolonies

genomicDNA

kb

1.0 -0.6 -

Fig.1

ADVANTAGES

Saves time

Easy to use

Ideal for 96-well PCR


Rapid-Load™ PCR Loading Dye, 5 mL RL-105 $25

Rapid-Load™ PCR Loading Dye, 25 mL RL-125 $100

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To Order

E-Commerce: www.origene.com

Telephone: 1-888-267-4436 (toll-free in U.S. only)

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Address: 6 Taft Court, Suite 100

Rockville, MD 20850

Please include the following information when ordering via fax:

1. Name and telephone number of person placing order

2. Catalog number, description, and quantity of item ordered

3. Purchase order number or credit card* information

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5. Billing address

*For your convenience, we accept VISA®, MasterCard® and AmericanExpress®. To order using a credit card,

please supply the cardholder name (as it appears on the card), the account number, and the expiration date.

By placing an order with OriGene, you agree to the following:

PRICINGPrices are subject to change without notice. For current pricing information on any product, please contact

OriGene Customer Service.

PAYMENT TERMSNet 30 days from the date of invoice in U.S. dollars.

PARTIAL SHIPMENTAll orders are normally filled within a single shipment. If this is not possible, a partial shipment will be sent

unless otherwise indicated when the order is placed.

RETURNS AND CREDITSOriGene products may not be returned without prior authorization from Customer Service (1-301-340-3188).

For returns due to damaged products or missing components, please obtain an authorization number and

instructions for returning the goods to the Customer Service Department. The products must be returned in

their original, intact condition to receive credit. In the event of returns due to customer ordering error, there

will be a 20% restocking fee and shipping charges will not be credited.

CONDITIONSAll OriGene products are for research use only and are not intended for drug, diagnostic, or human use. The

resale, modification or duplication for resale, or any other transfer for value of intact, duplicated or modified

product, is strictly prohibited. OriGene products may be protected by patents, pending patent applications,

and/or copyrights.

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SHIPPINGProducts are shipped F.O.B. Rockville, Maryland. Shipping charges are prepaid and added to your invoice.

Domestic orders requiring shipment on dry ice are delivered via Federal Express Priority Overnight Service,

Monday through Thursday, unless otherwise requested.

CUSTOM AND BULK ORDERSPlease request quotations for all custom and bulk orders by calling OriGene at 1-301-340-3188 or 1-888-267-4436

(toll-free in U.S. only).

LIMITED PRODUCT WARRANTYOriGene Technologies, Inc. warrants that the product will meet specifications listed. At OriGene’s discretion,

free replacement of any nonconforming product will be made if OriGene is notified within 30 days of product

receipt. This warranty limits OriGene’s liability to the cost of replacement of the product in question only.

OriGene provides no other warranty, express or implied, and is not liable or responsible for any indirect or

incidental damages or loss as a consequence of product use. No warranty is given for products used after the

printed expiration date or for products not stored or used according to the product use specifications given. If

you experience difficulty with any OriGene product, please contact OriGene Technical Support promptly at

1-301-340-3188, ext. 2 or 1-888-267-4436, ext. 2.

PCR is covered by patents issued to Cetus Corporation and owned and licensed by Hoffmann-La Roche, Inc. Purchase of any of OriGene’s PCR-related products does not convey a licenseto use the PCR process covered by these patents. Purchasers of these products must obtain a license to use the PCR process before performing PCR.

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