translocation of carbon from surface organic horizons to ... file1 table s1. list of compounds...

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Electronic Supplementary Material For:

Translocation of carbon from surface organic horizons to the subsoil in coarse-textured Spodosols: Implications for deep soil C dynamics

David E. Rothstein1*, Ehsan R. Toosi2, Randall J. Schaetzl3, A. Stuart Grandy4

1Department of Forestry, Michigan State University, East Lansing MI, USA 48824

2Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI, USA 48824

3Department of Geography, Michigan State University, East Lansing, MI, USA 48824

4Department of Natural Resources and the Environment, University of New Hampshire, Durham, NH, USA 03824

*Corresponding author, Ph: 517-432-3353, Fax: 517-432-1143, Email: [email protected]

Doi:10.2136/sssaj2018.01.0033

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Table S1. List of compounds identified in dissolved organic matter samples from red pine and sugar maple stands organized by compound class. Vector direction cosines and significance refer to the ordination plotted in Figure 6. Significance is indicated as follows ***, P<0.001; **, P<0.01; *, P<0.05; †, P<0.1, NS, P>0.01.

Compound Origin

Axis 1 Vector

Direction Cosine

Axis 2 Vector

Direction Cosine Significance

Benzene, 2-propenyl- Aromatic 0.872 -0.490 †

Phenol, 2,4-bis(1,1-dimethylethyl)- Aromatic 0.872 -0.490 †

Benzene Aromatic 0.657 0.754 †

m-xylene Aromatic -0.528 -0.849 *

Acetophenone Aromatic 0.999 0.043 **

Biphenyl Aromatic 0.998 -0.066 **

Benzene, 1-ethenyl-3-methyl- Aromatic 0.926 -0.378 **

Benzene, propyl- Aromatic 0.684 -0.729 **

Benzene, (1-methylethyl)- Aromatic 0.620 -0.784 **

Benzene, 1,2,3-trimethyl- Aromatic -0.361 -0.932 **

Benzene, 1,2-diethyl- Aromatic -0.465 -0.885 **

Benzene, 1,2,3,4-tetramethyl- Aromatic 0.021 -1.000 ***

Naphthalene, 1,2-dihydro-4-methyl- Aromatic -0.137 -0.991 ***

Phenol, 3,4-dimethyl- Aromatic -0.434 -0.901 ***

2

Benzene, butyl- Aromatic 0.810 -0.587 NS

Naphthalene Aromatic 0.518 0.856 NS

Oxirane, ethenyl- Aromatic 0.247 -0.969 NS

Benzofuran Aromatic -0.252 0.968 NS

Anthracene Aromatic -0.315 -0.949 NS

Benzaldehyde Aromatic -0.426 -0.905 NS

2H-1-Benzopyran-2-one Aromatic -0.822 0.569 NS

Fluorene Aromatic -0.892 -0.452 NS

Ethanone, 1-(3,4-dimethoxyphenyl)- Lignin 0.630 0.777 †

Benzene, 4-ethenyl-1,2-dimethoxy- Lignin -0.966 0.257 †

Benzaldehyde, 4-hydroxy-3-methoxy- (Vanillin) Lignin -0.991 0.137 *

Ethylphenol Lignin -0.012 -1.000 **

Phenol, 2-methoxy-4-(1-propenyl)- (4-Isoeugenol) Lignin -0.884 0.468 **

Phenol, 2-Methoxy-4-vinyl- (Vinylguaiacol) Lignin -0.897 0.442 **

Phenol, 2-methoxy-4-methyl- (4-Methylguaiacol) Lignin -0.935 0.355 ** Benzoic acid, 4-hydroxy-3-methoxy-, methyl ester (Vanillic Acid, methyl est Lignin -0.942 0.336 **

Phenol, 4-ethyl-2-methoxy- (Ethylguaiacol) Lignin -0.994 0.109 **

3

Ethanone, 1-(4-hydroxy-3-methoxyphenyl)- (Acetovanillone) Lignin -0.901 0.434 ***

2-Propanone, 1-(4-hydroxy-3-methoxyphenyl)- (Guaiacylacetone) Lignin -0.970 0.244 ***

Benzoic acid, methyl ester Lignin -0.999 -0.048 ***

Phenol, 2,6-dimethoxy- (Syringol) Lignin 0.326 0.945 NS

Benzoic acid, 4-hydroxy-3-methoxy-Vanillic acid) Lignin 0.029 1.000 NS

Ethanone, 1-(4-hydroxy-3,5-dimethoxyphenyl)- (Acetosyringone) Lignin 0.029 1.000 NS

Benzene, 1-ethenyl-4-methoxy- Lignin -0.259 -0.966 NS

Phenol, 2-methoxy- (Guaiacol) Lignin -0.512 0.859 NS

3,4-Dimethoxytoluene Lignin -0.744 0.669 NS

Phenol, 2-methoxy-4-propyl- (4-Guaiacylpropane) Lignin -0.744 0.669 NS

Phenol, 2-methoxy-4-(2-propenyl)- (4-Eugenol) Lignin -0.989 0.150 NS

1-Heptene Lipid 0.950 -0.313 †

n-Pentadecane Lipid 0.872 -0.490 †

n-Tetracosane Lipid 0.411 0.911 †

1-Hexene, 3-methyl- Lipid 0.991 -0.133 *

n-Heptane Lipid 0.463 -0.886 **

Hex-2-yn-4-one, 2-methyl- Lipid 0.144 -0.990 ***

4

1-Pentene, 3-ethyl-2-methyl- Lipid 0.021 -1.000 ***

n-Nonane Lipid 0.852 -0.524 NS

3-Decene Lipid 0.590 -0.807 NS

n-Heptacosane (C27) Lipid 0.473 0.881 NS

2-Propyn-1-amine Lipid 0.471 0.882 NS

n-Hentriacontane (C31) Lipid 0.471 0.882 NS

n-Nonacosane (C29) Lipid 0.471 0.882 NS

n-Octacosane Lipid 0.471 0.882 NS

1,3-Butadiene Lipid -0.372 -0.928 NS

Isocrotonic acid Lipid -0.591 -0.807 NS

Propene Lipid -0.634 -0.773 NS

1H-Pyrazole, 4,5-dihydro-1,5-dimethyl- N-Bearing 0.872 -0.490 †

Acetonitrile, (dimethylamino)- N-Bearing 0.872 -0.490 †

Ethanone, 1-(1-methyl-1H-pyrrol-2-yl)- N-Bearing 0.822 -0.570 †

2,5-Furandione, 3-methyl- N-Bearing -0.975 -0.224 †

4-Pyridinecarboxaldehyde N-Bearing 0.991 -0.137 *

Pyridine, 2-ethyl N-Bearing 0.696 -0.718 *

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Propane, 2-nitro- N-Bearing 0.490 -0.872 *

N-Butyl-tert-butylamine N-Bearing -0.907 0.421 *

Acetamide, N-hydroxy N-Bearing -0.955 0.297 *

Aniline N-Bearing 0.884 0.468 **

Piperidine-2,5-dione N-Bearing 0.616 0.788 **

1H-Pyrrole-2-carboxaldehyde N-Bearing 0.518 0.855 **

Pyrazolo[5,1-c][1,2,4]benzotriazin-8-ol N-Bearing 0.297 0.955 **

Pyridine 3-methyl N-Bearing 0.971 -0.239 NS

2-Propenenitrile, 2-methyl- N-Bearing 0.928 0.372 NS

p-Aminotoluene N-Bearing 0.926 -0.379 NS

2-Pyridinecarbonitrile N-Bearing 0.865 0.501 NS

1H-1,2,4-Triazole N-Bearing 0.743 -0.670 NS

Diethyltoluamide N-Bearing 0.720 0.694 NS

5H-1-Pyrindine N-Bearing 0.696 0.718 NS

Hexanedinitrile N-Bearing 0.587 0.809 NS

3-Phenylpyridine N-Bearing 0.584 0.812 NS

2-Amino-4-methylpyrimidine N-Bearing 0.349 0.937 NS

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Pyridine, 4-methoxy- N-Bearing 0.029 1.000 NS

3-Methylpyridazine N-Bearing -0.574 0.819 NS

3-Pyridinol N-Bearing -0.621 -0.784 NS

2-Pyridinealdehyde N-Bearing -0.634 -0.773 NS

1H-Tetrazole, 1-methyl- N-Bearing -0.985 0.174 NS

o-xylene Phenol 0.872 -0.490 †

Phenol, 4-methyl- Phenol -0.103 -0.995 †

p-isopropenylphenol Phenol 0.967 0.255 NS

Benzene, 1,4-dimethoxy- Phenol -0.744 0.669 NS

2H-Pyran-2-one Polysaccharide -0.429 0.903 †

2(3H)-Benzofuranone, 3-methyl- Polysaccharide -0.633 -0.774 †

Benzofuran, 2,3-dihydro- Polysaccharide -0.941 0.340 †

2(3H)-Furanone, 5-methyl- Polysaccharide -0.991 0.134 †

Furfural Polysaccharide -0.541 0.841 *

Levoglucosenone Polysaccharide -0.878 0.480 *

2-Cyclopenten-1-one, 3-methyl- Polysaccharide 0.192 -0.981 **

Cyclopent-2-ene-1-one, 2,3,4-trimethyl- Polysaccharide -0.020 -1.000 **

7

2(5H)-Furanone Polysaccharide -0.836 0.548 **

2-Cyclopenten-1-one, 2-methyl- Polysaccharide 0.236 -0.972 ***

5-Ethyl-2-furaldehyde Polysaccharide 0.138 -0.990 ***

2-Cyclopenten-1-one, 2,3-dimethyl- Polysaccharide 0.076 -0.997 ***

PENTANAL Polysaccharide 0.011 -1.000 ***

Methyl 2-furoate Polysaccharide -0.744 0.669 N

Cyclopentanone Polysaccharide 0.992 -0.126 NS

Cyclopropanecarboxaldehyde, methylene- Polysaccharide 0.938 -0.348 NS

3-Furaldehyde Polysaccharide 0.296 0.955 NS

Butanal, 2-methyl- Polysaccharide -0.096 0.995 NS

2-Acetylfuran Polysaccharide -0.136 0.991 NS

Furan, 2-ethyl-5-methyl- Polysaccharide -0.242 0.970 NS

Furfural, 5-methyl- Polysaccharide -0.244 0.970 NS

Levoglucosan Polysaccharide -0.277 0.961 NS

1,2-Cyclohexanedione Polysaccharide -0.315 -0.949 NS

Vinylfuran Polysaccharide -0.371 0.929 NS

Furan, 2-ethyl- Polysaccharide -0.438 0.899 NS

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4H-Pyran-4-one, 3-hydroxy-2-methyl- Polysaccharide -0.453 0.892 NS

2(5H)-Furanone, 5-methyl- Polysaccharide -0.552 0.834 NS

2-Cyclopenten-1-one, 2-hydroxy-3-methyl- Polysaccharide -0.568 0.823 NS

Acetic anhydride Polysaccharide -0.682 0.731 NS

Furan, 2,5-dimethyl- Polysaccharide -0.812 0.584 NS

2-Furanmethanol Polysaccharide -0.989 0.150 NS

Furan, 2,3,5-trimethyl- Polysaccharide -0.995 0.101 NS

Benzofuran, 2-methyl- Polysaccharide -1.000 -0.027 NS

4-Pyridinamine Protein 1.000 -0.012 *

Ethylbenzene Protein 0.483 -0.876 *

1H-Pyrrole, 2-methyl- Protein 0.990 0.143 **

Benzonitrile Protein 0.745 0.668 **

Pyridine Protein 0.609 0.794 **

3-Methylindole Protein 0.107 -0.994 **

Indole Protein 0.908 0.420 ***

Benzyl nitrile Protein 0.856 0.517 ***

Pyrrole Protein 0.839 0.544 ***

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1 H-Pyrrole, 2-ethyl- Protein 0.867 -0.499 NS

Benzenepropanenitrile Protein 0.743 -0.670 NS

Pyridine, 3,5-dimethyl- Protein 0.444 0.896 NS

Pyridine Protein -0.021 -1.000 NS

Propanoic acid, 2-hydroxy-, methyl ester, (Ò)-

Unknown Origin 0.872 -0.490 †

Phenol, 2-methyl-4-(1,1,3,3-tetramethylbutyl)-

Unknown Origin 0.872 -0.490 †

C11_H12 Unknown Origin 0.763 -0.647 †

3-Methylthiophene Unknown Origin 0.749 0.663 †

3-Penten-2-one, (E)- Unknown Origin 0.498 0.867 †

2-Pentanone Unknown Origin -0.151 -0.988 †

1,2-Benzenediol, 3-methoxy- Unknown Origin -0.888 0.460 †

Phenol, 4-(1,1,3,3-tetramethylbutyl)- Unknown Origin 0.947 -0.320 *

C8_H16 Unknown Origin 0.839 -0.544 *

1H-Inden-1-one, 2,3-dihydro- Unknown Origin -0.127 -0.992 *

Phosphonic acid, (p-hydroxyphenyl)- Unknown Origin -0.290 0.957 *

Mequinol Unknown Origin -0.999 0.055 *

1,3,5,7-Cyclooctatetraene Unknown Origin 0.929 -0.371 **

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Methanesulfonic acid, methyl ester Unknown Origin 0.804 0.594 **

1-Undecanol Unknown Origin 0.493 -0.870 **

7-Methylindan-1-one Unknown Origin -0.078 -0.997 **

Trimethylphenol Unknown Origin -0.314 -0.949 ***

2,3,5-Trimethylnaphthalene Unknown Origin -0.736 -0.677 ***

Toluene Unknown Origin 0.932 0.361 NS

Thiophene Unknown Origin 0.847 0.531 NS

Indane Unknown Origin 0.743 -0.669 NS

C9_H8 Unknown Origin 0.719 -0.695 NS

2H-1-Benzopyran-3,4-diol, 2-(3,4-dimethoxyphenyl)

Unknown Origin 0.501 0.866 NS

Thiocyanic acid, methyl ester Unknown Origin 0.471 0.882 NS

2-Methylthiophene Unknown Origin 0.415 0.910 NS

Resorcinol (Dihydroxybenzene) Unknown Origin 0.159 0.987 NS

Hydroquinone Unknown Origin -0.163 0.987 NS

2-Cyclohexen-1-one Unknown Origin -0.245 0.969 NS

Spiro[2.4]hepta-4,6-diene Unknown Origin -0.315 -0.949 NS

2,3,6-Trimethylnaphthalene Unknown Origin -0.420 -0.907 NS

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1,3,5-Cyclooctatriene Unknown Origin -0.567 -0.824 NS

2-Butenoic acid, methyl ester, (E)- Unknown Origin -0.634 -0.773 NS

Benzene, 2,4-diisocyanato-1-methyl- Unknown Origin -0.650 -0.760 NS

Pyruvaldehyde Unknown Origin -0.778 0.629 NS

1,3,5-Cycloheptatriene Unknown Origin -0.836 -0.549 NS

Dimethylbenzofuran Unknown Origin -0.941 -0.339 NS

Beta-Pinene Unknown Origin -1.000 0.002 NS

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Table S2. List of compounds identified in dissolved organic matter, soil and root samples samples from red pine stands organized by compound class. Vector direction cosines and significance refer to the ordination plotted in Figure 7A. Significance is indicated as follows ***, P<0.001; **, P<0.01; *, P<0.05; †, P<0.1, NS, P>0.01.

Compound Origin

Axis 1 Vector

Direction Cosine

Axis 2 Vector


Benzene Aromatic -0.538 0.843 †

Benzene, butyl- Aromatic -0.413 0.911 *

Phenol, 3,4-dimethyl- Aromatic -0.403 -0.915 *

Phenol, 2,4-bis(1,1-dimethylethyl)- Aromatic -0.313 0.950 *

Benzene Aromatic -0.271 0.963 *

Benzofuran Aromatic -0.993 -0.116 **

Benzene, 1,2,3,4-tetramethyl- Aromatic -0.914 -0.405 **

2H-1-Benzopyran-2-one Aromatic -0.575 -0.818 **

Naphthalene, 1,2-dihydro-4-methyl- Aromatic -0.992 -0.128 ***

Benzene, 1,2,3-trimethyl- Aromatic -0.820 -0.572 ***

Fluorene Aromatic -0.659 0.752 ***

Benzene, (1-methylethyl)- Aromatic -0.634 -0.773 ***

m-xylene Aromatic -0.601 -0.799 ***

Naphthalene Aromatic -0.591 0.807 ***

Benzene, 1-ethenyl-3-methyl- Aromatic -0.557 -0.831 ***

13

Anthracene Aromatic -0.519 0.855 ***

Biphenyl Aromatic -0.501 0.866 ***

Benzene, 1,2-diethyl- Aromatic -0.475 -0.880 ***

Benzene, hexyl- Aromatic -0.442 0.897 ***

Benzene, propyl- Aromatic -0.428 0.904 ***

Acetophenone Aromatic -0.292 -0.956 ***

Benzene, 2-propenyl- Aromatic -0.228 0.974 ***

Oxirane, ethenyl- Aromatic -0.273 -0.962 NS

Benzene, (1,3-dimethylbutyl)- Aromatic -0.176 0.984 NS

Benzaldehyde Aromatic 0.068 -0.998 NS

Phenol, 3-methyl- Aromatic 0.964 0.267 NS

p-Xylene Aromatic 0.981 0.194 NS Ethanone, 1-(4-hydroxy-3,5-dimethoxyphenyl)- (Acetosyringone) Lignin -0.538 0.843 †

Phenol, 4-ethyl-2,6-dimethoxy- (Ethylsyringol) Lignin -0.538 0.843 † Ethanone, 1-(4-hydroxy-3-methoxyphenyl)- (Acetovanillone) Lignin 0.324 -0.946 * Benzoic acid, 4-hydroxy-3-methoxy-Vanillic acid) Lignin 0.999 0.035 *

Benzoic acid, methyl ester Lignin -0.085 -0.996 **

Ethylphenol Lignin -0.912 -0.410 *** 2-Propanone, 1-(4-hydroxy-3-methoxyphenyl)- (Guaiacylacetone) Lignin 0.924 -0.382 ***

Benzaldehyde, 4-hydroxy-3-methoxy- (Vanillin) Lignin 0.930 -0.369 *** Benzeneacetic acid, 4-hydroxy-3-methoxy- (Homovanillic acid) Lignin 0.963 0.269 ***

14

Phenol, 2-methoxy-4-propyl- (4-Guaiacylpropane) Lignin 0.969 0.249 ***

1,2-Benzenediol, 4-methyl- (Homocatechol) Lignin 0.982 0.191 ***

Phenol, 4-ethyl-2-methoxy- (Ethylguaiacol) Lignin 0.991 0.134 *** Phenol, 2-methoxy-4-(1-propenyl)- (4-Isoeugenol) Lignin 0.998 0.066 ***

Phenol, 2-Methoxy-4-vinyl- (Vinylguaiacol) Lignin 0.999 0.033 *** Phenol, 2-methoxy-4-methyl- (4-Methylguaiacol) Lignin 1.000 0.012 ***

Phenol, 2-methoxy- (Guaiacol) Lignin 0.015 -1.000 NS Phenol, 2-methoxy-4-(1Z)-1-propen-1-yl- (cis-Isoeugenol) Lignin 0.988 0.155 NS Phenol, 2,6-dimethoxy-4-(2-propenyl)- (Methoxyeugenol) Lignin 0.997 -0.075 NS

Phenol, 2,6-dimethoxy- (Syringol) Lignin 0.888 0.459 NS

Phenol, 2-methoxy-4-(2-propenyl)- (4-Eugenol) Lignin 0.995 -0.099 NS Benzoic acid, 4-hydroxy-3-methoxy-, methyl ester (Vanillic Acid, methyl ester) Lignin 0.690 -0.724 *

Benzene, 1-ethenyl-4-methoxy- Lignin -0.983 -0.183 **

Benzene, 4-ethenyl-1,2-dimethoxy- Lignin 0.998 0.070 ***

Benzene, methoxy- Lignin -0.538 0.843 †

Ethanone, 1-(3,4-dimethoxyphenyl)- Lignin 0.976 -0.217 NS

Benzaldehyde 3,4-dimethoxy Lignin 0.981 0.194 NS

Benzene, 1,2-dimethoxy- Lignin 0.981 0.194 NS

Benzene, 1-methoxy-4-methyl- Lignin 0.894 0.448 NS

3,4-Dimethoxytoluene Lignin 0.998 0.069 NS

1-Butyne, 3,3-dimethyl- Lipid -0.429 0.903 †

15

C14_alkene_#3 Lipid -0.228 0.974 †

n-Heptane Lipid 0.596 0.803 † Hexadecanoic acid, methyl ester (Palmitic acid-C16) Lipid -0.409 0.913 *

3-Decene Lipid 0.047 0.999 *

n-Tetradecane Lipid -0.567 0.824 **

n-Dodecane Lipid -0.562 0.827 **

n-Docosane (C22) Lipid -0.558 0.830 **

n-Octadecane Lipid -0.509 0.861 **

n-Decane Lipid -0.415 0.910 **

n-Octane Lipid -0.247 0.969 **

n-Nonane Lipid -0.200 0.980 **

Hex-2-yn-4-one, 2-methyl- Lipid -0.850 -0.527 ***

n-Hexadecane Lipid -0.476 0.880 ***

1,3-Butadiene Lipid -0.463 -0.886 ***

n-Heptadecane Lipid -0.456 0.890 ***

n-Tridecane Lipid -0.412 0.911 ***

7-Tetradecene Lipid -0.409 0.912 ***

1-Heptene Lipid -0.406 0.914 ***

n-Pentadecane Lipid -0.367 0.930 ***

1-Hexene, 3-methyl- Lipid -0.326 0.945 ***

Isocrotonic acid Lipid -0.973 -0.232 NS

16

n-Undecane Lipid -0.297 0.955 NS

1-Pentene, 3-ethyl-2-methyl- Lipid -0.035 -0.999 NS

1,3-Octadiene Lipid 0.026 1.000 NS

2-Butenedioic acid, 2-methyl-, (E)- Lipid 0.172 0.985 NS

9-Hexadecenoic acid, methyl ester, (Z)- Lipid 0.780 0.626 NS

2-Propyn-1-amine Lipid 0.988 0.155 NS

Propene Lipid 0.670 -0.742 NS

n-Heneicosane (C21) Lipid 0.981 0.194 NS

2-Pyridinecarbonitrile N-Bearing -0.574 0.819 †

4(1H)-Pyridinone, 2,3-dihydro-1-methyl- N-Bearing -0.538 0.843 †

4-Amino-2(1H)-pyridinone N-Bearing -0.384 0.923 †

3-Pyridinol N-Bearing -0.116 0.993 †

Piperidine-2,5-dione N-Bearing -0.503 0.864 *

Hexanedinitrile N-Bearing -0.418 0.909 *

Ethanone, 1-(1-methyl-1H-pyrrol-2-yl)- N-Bearing -0.290 0.957 *

5H-1-Pyrindine N-Bearing -0.840 0.543 **

Pyridine, 2-ethyl N-Bearing -0.785 0.620 **

3-Pyridinecarbonitrile N-Bearing -0.562 0.827 **

Acetamide, N-(2,4-dihydroxyphenyl)- N-Bearing -0.285 0.959 **

2,5-Furandione, 3-methyl- N-Bearing -0.204 -0.979 **

N-Butyl-tert-butylamine N-Bearing 0.940 0.341 **

17

4-Pyridinecarboxaldehyde N-Bearing -0.742 0.670 ***

Pyridine 3-methyl N-Bearing -0.643 0.766 ***

Aniline N-Bearing -0.541 0.841 ***

1H-Pyrrole, 3-methyl- N-Bearing -0.490 0.872 ***

3-Phenylpyridine N-Bearing -0.383 0.924 ***

1H-Pyrrole-2-carboxaldehyde N-Bearing -0.165 0.986 ***

Pyrazolo[5,1-c][1,2,4]benzotriazin-8-ol N-Bearing -0.044 0.999 ***

Alpha-amino-gamma-butyrolactone N-Bearing 0.998 0.066 ***

Propane, 2-nitro- N-Bearing -0.663 0.748 NS

2-Pyridinealdehyde N-Bearing -0.617 0.787 NS

p-Aminotoluene N-Bearing -0.956 -0.295 NS


1H-Pyrrole-2-carboxaldehyde, 1-methyl- N-Bearing -0.215 0.977 NS

2-(N-Methyl-N-ethylamino)phenol N-Bearing -0.215 0.977 NS

1H-Tetrazole, 1-methyl- N-Bearing -0.150 -0.989 NS

Pyridine, 4-methoxy- N-Bearing -0.058 0.998 NS

Diethyltoluamide (DEET) N-Bearing 0.101 0.995 NS

1,4-Benzenediamine N-Bearing 0.338 0.941 NS

Acetamide, N-hydroxy N-Bearing 0.695 -0.719 NS

Pyrazine, 2-methoxy-3-(1-methylpropyl)- N-Bearing 0.780 0.626 NS

p-isopropenylphenol Phenol 0.981 0.192 †

18

Phenol, 4-methyl- Phenol -0.999 0.054 ***

Phenol Phenol -0.378 0.926 ***

Benzene, 1,4-dimethoxy- Phenol -0.123 -0.992 NS

Benzene, 1-ethyl-4-methoxy- Phenol 0.862 0.506 NS

1,2-Cyclopentanedione Polysaccharide -0.142 0.990 †

2(5H)-Furanone Polysaccharide 0.397 0.918 †

Furfural, 5-methyl- Polysaccharide -0.363 -0.932 *

3-Acetamidofuran Polysaccharide -0.351 0.937 *

Butanal, 2-methyl- Polysaccharide -0.011 1.000 *

Furan, 2,5-dimethyl- Polysaccharide 0.132 -0.991 *

Furfural Polysaccharide 0.706 0.708 *

Benzofuran, 2-methyl- Polysaccharide -0.945 -0.328 **

PENTANAL Polysaccharide -0.431 -0.903 **

Cyclopentanone Polysaccharide -0.335 0.942 **

2(3H)-Furanone, 5-methyl- Polysaccharide 0.025 -1.000 **

Levoglucosan Polysaccharide 0.633 0.774 **

Acetic anhydride Polysaccharide 0.977 -0.215 **

2-Cyclopenten-1-one, 2-methyl- Polysaccharide -0.894 -0.449 ***

Cyclopent-2-ene-1-one, 2,3,4-trimethyl- Polysaccharide -0.693 -0.721 ***

5-Ethyl-2-furaldehyde Polysaccharide -0.648 -0.761 ***

2-Cyclopenten-1-one, 2,3-dimethyl- Polysaccharide -0.646 -0.763 ***

19

Furan, 2,3,5-trimethyl- Polysaccharide -0.541 -0.841 ***

Furan, 2,4-dimethyl- Polysaccharide -0.476 0.879 ***


2(3H)-Benzofuranone, 3-methyl- Polysaccharide -0.361 -0.932 ***

Benzofuran, 2,3-dihydro- Polysaccharide 0.962 0.271 ***

2-Cyclopenten-1-one, 2-hydroxy-3-methyl- Polysaccharide 1.000 0.028 ***

Furan, 2-ethyl- Polysaccharide -0.918 0.398 NS

3-Furaldehyde Polysaccharide -0.341 0.940 NS

2-Acetylfuran Polysaccharide -0.311 -0.950 NS

Levoglucosenone Polysaccharide -0.122 0.993 NS

Furan, 2-ethyl-5-methyl- Polysaccharide 0.125 0.992 NS

2(5H)-Furanone, 5-methyl- Polysaccharide 0.859 -0.512 NS

Methyl 2-furoate Polysaccharide 0.593 -0.805 NS

2H-Pyran-2-one Polysaccharide 0.438 -0.899 NS

1,2-Cyclohexanedione Polysaccharide 0.865 -0.502 NS

Furan, 3-methyl- Polysaccharide 0.953 -0.305 NS

1,2-Cyclopentanedione, 3-methyl- Polysaccharide 0.892 0.452 NS

Vinylfuran Polysaccharide 0.983 0.184 NS

2-Furanmethanol Polysaccharide 0.744 -0.668 NS

Acetic acid Polysaccharide 0.999 0.037 NS

Cyclopropanecarboxaldehyde, methylene- Polysaccharide 0.994 0.112 NS

20

4H-Pyran-4-one, 3-hydroxy-2-methyl- Polysaccharide 0.971 0.240 NS

1H-Pyrrole, 1-methyl- Protein -0.573 0.819 †

Pyridine, 3,5-dimethyl- Protein -0.501 0.865 *

1 H-Pyrrole, 2-ethyl- Protein -0.444 0.896 *

Benzyl nitrile Protein -0.309 0.951 **

Ethylbenzene Protein -0.962 0.272 ***

4-Pyridinamine Protein -0.704 0.710 ***

1H-Pyrrole, 2-methyl- Protein -0.673 0.739 ***

Pyridine Protein -0.595 0.804 ***

Benzonitrile Protein -0.580 0.815 ***

Pyrrole Protein -0.512 0.859 ***

Benzenepropanenitrile Protein -0.485 0.874 ***

Indole Protein -0.412 0.911 ***

3-Methylindole Protein -0.387 0.922 ***


Styrene Protein 0.993 0.120 NS

3-Methylthiophene Unknown Origin -0.973 0.232 †

Benzene, heptyl- Unknown Origin -0.574 0.819 †

Benzene, pentyl- Unknown Origin -0.538 0.843 †

2,3,5-Trimethylnaphthalene Unknown Origin 0.170 -0.985 †

Resorcinol (Dihydroxybenzene) Unknown Origin 0.810 0.586 †

21

2,3,6-Trimethylnaphthalene Unknown Origin -0.767 0.642 *

1,3,5-Cyclooctatriene Unknown Origin -0.456 -0.890 *

4-Hydroxy-2-methylacetophenone Unknown Origin -0.040 0.999 *

Mequinol Unknown Origin 0.686 -0.728 *

Propanoic acid, 2-hydroxy-, methyl ester, (Ò)- Unknown Origin 0.993 -0.118 *

Pyruvaldehyde Unknown Origin 0.997 -0.083 *

C9_H8 Unknown Origin -0.781 0.624 **

C11_H12 Unknown Origin -0.577 0.817 **

Pyrene Unknown Origin -0.572 0.820 **

3-Penten-2-one, (E)- Unknown Origin -0.482 -0.876 **

Trimethylphenol Unknown Origin -0.460 -0.888 **

1,3,5-Cycloheptatriene Unknown Origin -0.033 0.999 **

1H-Inden-1-one, 2,3-dihydro- Unknown Origin -0.914 -0.406 ***

Toluene Unknown Origin -0.791 0.612 ***

2-Pentanone Unknown Origin -0.642 -0.767 ***

7-Methylindan-1-one Unknown Origin -0.616 -0.787 ***

Indane Unknown Origin -0.616 0.788 ***

Acenaphthene Unknown Origin -0.569 0.822 ***

Fluoranthene Unknown Origin -0.567 0.824 ***

1,3,5,7-Cyclooctatetraene Unknown Origin -0.529 0.849 ***

Dimethylbenzofuran Unknown Origin -0.502 -0.865 ***

22

Methanesulfonic acid, methyl ester Unknown Origin -0.458 0.889 ***

1-Undecanol Unknown Origin -0.384 0.923 ***

Phosphonic acid, (p-hydroxyphenyl)- Unknown Origin -0.076 -0.997 ***

C8_H16 Unknown Origin -0.065 0.998 ***

D-Limonene Unknown Origin 0.999 0.050 ***

Spiro[2.4]hepta-4,6-diene Unknown Origin -0.999 -0.033 NS


Phenol, 4-(1,1,3,3-tetramethylbutyl)- Unknown Origin -0.512 -0.859 NS

2-Butenoic acid, methyl ester, (E)- Unknown Origin -0.443 -0.897 NS

2-Propen-1-ol Unknown Origin -0.274 0.962 NS

2-Propenoic acid, ethenyl ester Unknown Origin -0.336 0.942 NS

Hydroquinone Unknown Origin -0.167 0.986 NS

1-Propanone, 1-cyclopropyl Unknown Origin 0.993 0.120 NS

Beta-Pinene Unknown Origin 0.687 0.727 NS

3-Hexanol, 2,4-dimethyl- Unknown Origin 1.000 0.002 NS

Vinyl crotonate Unknown Origin 0.999 0.037 NS

2-Naphthalenol, 3-methoxy- Unknown Origin 0.981 0.194 NS

2-Propenoic acid, anhydride Unknown Origin 0.981 0.194 NS


Unknown Origin 0.989 0.150 NS

1,2-Benzenediol, 3-methoxy- Unknown Origin 0.857 -0.516 NS

Acetic acid, cyano-, ethyl ester Unknown Origin 0.998 -0.065 NS

23

2-Methoxy-5-methylphenol Unknown Origin 0.983 0.185 NS

Phenol, 2-methoxy-5-(1-propenyl)-, (E)- Unknown Origin 0.999 0.036 NS

Ethanone, 1-(3-hydroxy-4-methoxyphenyl)- Unknown Origin 0.935 0.355 NS

2-Butanone, 3,3-dimethyl- Unknown Origin 0.966 0.259 NS

24

Table S3. List of compounds identified in dissolved organic matter, soil and root samples from sugar maple stands organized by compound class. Vector direction cosines and significance refer to the ordination plotted in Figure 7C. Significance is indicated as follows ***, P<0.001; **, P<0.01; *, P<0.05; †, P<0.1, NS, P>0.01.

Compound Origin

Axis 1 Vector

Direction Cosine

Axis 2 Vector


Benzene, propyl- Aromatic -0.579 0.815 *

Naphthalene, 1,2-dihydro-4-methyl- Aromatic -0.705 0.710 *

Benzene Aromatic -0.955 -0.298 **

Biphenyl Aromatic -0.685 0.729 **

2H-1-Benzopyran-2-one Aromatic -0.600 -0.800 **

Benzene, (1-methylethyl)- Aromatic -0.509 -0.861 **

Fluorene Aromatic -0.434 0.901 **

Benzene, hexyl- Aromatic -0.209 0.978 **

Acetophenone Aromatic -0.392 -0.920 ***

Benzene, 1-ethenyl-3-methyl- Aromatic -0.478 -0.878 ***

m-xylene Aromatic -0.590 -0.807 ***

Benzene, 1,2-diethyl- Aromatic -0.662 -0.750 ***

Benzofuran Aromatic -0.761 -0.649 ***

Benzene, 1,2,3-trimethyl- Aromatic -0.964 0.266 ***

Naphthalene Aromatic -0.738 0.674 ***

25

Anthracene Aromatic -0.355 0.935 ***

Phenol, 2,4-bis(1,1-dimethylethyl)- Aromatic -0.222 0.975 ***

Benzene, 1,2,3,4-tetramethyl- Aromatic -0.486 0.874 NS

Phenol, 3-methyl- Aromatic -0.336 0.942 NS

Benzene, 2-propenyl- Aromatic -0.107 0.994 NS

Benzene Aromatic 0.744 -0.668 NS

Benzaldehyde Aromatic -0.804 -0.595 NS

Benzene, (1,3-dimethylbutyl)- Aromatic -0.257 0.966 NS

Oxirane, ethenyl- Aromatic 0.037 -0.999 NS

Benzene, butyl- Aromatic -0.415 0.910 NS

Phenol, 3,4-dimethyl- Aromatic 0.969 -0.247 NS

Phenol, 2-methoxy- (Guaiacol) Lignin -0.322 -0.947 †

Phenol, 2,6-dimethoxy- (Syringol) Lignin 0.719 -0.695 †

Benzaldehyde, 4-hydroxy-3,5-dimethoxy- (Syringaldehyde) Lignin 0.823 0.567 *

Ethylphenol Lignin -0.982 -0.188 **

Ethanone, 1-(4-hydroxy-3,5-dimethoxyphenyl)- (Acetosyringone) Lignin 0.928 0.372 **

Phenol, 2-methoxy-4-methyl- (4-Methylguaiacol) Lignin 0.919 -0.394 ***

1,2-Benzenediol, 4-methyl- (Homocatechol) Lignin 0.958 -0.288 ***

Phenol, 4-ethyl-2-methoxy- (Ethylguaiacol) Lignin 0.961 -0.278 ***

Phenol, 2-methoxy-4-propyl- (4-Guaiacylpropane) Lignin 0.971 -0.239 ***

26

Phenol, 2,6-dimethoxy-4-(2-propenyl)- (Methoxyeugenol) Lignin 0.974 0.228 ***

Benzeneacetic acid, 4-hydroxy-3-methoxy- (Homovanillic acid) Lignin 0.980 0.200 ***

Phenol, 2-Methoxy-4-vinyl- (Vinylguaiacol) Lignin 0.982 -0.190 ***

Phenol, 2-methoxy-4-(1-propenyl)- (4-Isoeugenol) Lignin 0.988 -0.157 ***

2-Propanone, 1-(4-hydroxy-3-methoxyphenyl)- (Guaiacylacetone) Lignin 0.992 -0.127 ***

Benzaldehyde, 4-hydroxy-3-methoxy- (Vanillin) Lignin 0.996 0.085 ***

Phenol, 4-ethyl-2,6-dimethoxy- (Ethylsyringol) Lignin 0.998 0.055 ***

Benzoic acid, 4-hydroxy-3-methoxy-Vanillic acid) Lignin 1.000 -0.022 ***

Benzoic acid, methyl ester Lignin -0.073 -0.997 NS

Ethanone, 1-(4-hydroxy-3-methoxyphenyl)- (Acetovanillone) Lignin 0.065 -0.998 NS

Phenol, 2-methoxy-4-(2-propenyl)- (4-Eugenol) Lignin 0.999 0.037 NS

Benzene, 1,2-dimethoxy- Lignin 0.813 -0.582 *

Benzene, 1-methoxy-4-methyl- Lignin 0.950 -0.313 * Benzoic acid, 4-hydroxy-3-methoxy-, methyl ester (Vanillic Acid, methyl est Lignin 0.864 -0.504 **

Benzene, 4-ethenyl-1,2-dimethoxy- Lignin 0.962 -0.275 ***

Benzene, 1,2-dimethoxy-4-(1-propen-1-yl)- (Methylisoeugenol) Lignin 0.866 -0.500 NS

Benzene, 1-ethenyl-4-methoxy- Lignin -0.913 0.408 NS

3,4-Dimethoxytoluene Lignin 0.853 0.521 NS

Ethanone, 1-(3,4-dimethoxyphenyl)- Lignin 0.870 0.494 NS

27

n-Decane Lipid -0.258 0.966 †

Isocrotonic acid Lipid -0.099 0.995 *

Hex-2-yn-4-one, 2-methyl- Lipid -0.998 0.061 *

1-Heptene Lipid -0.597 0.802 *

n-Tetracosane Lipid -0.364 -0.931 *

n-Triacontane (C30) Lipid 0.855 0.519 *

n-Octane Lipid -0.082 0.997 *

Hepta-2,4-dienoic acid, methyl ester Lipid -0.200 0.980 *

n-Tridecane Lipid -0.380 0.925 *

Propene Lipid 0.992 -0.127 **

n-Hexadecane Lipid -0.361 0.933 **

1-Hexene, 3-methyl- Lipid -0.209 0.978 **

2-Butenedioic acid, 2-methyl-, (E)- Lipid -0.121 0.993 **

7-Tetradecene Lipid -0.270 0.963 ***

n-Heptadecane Lipid -0.253 0.967 ***

Hexadecanoic acid, methyl ester (Palmitic acid-C16) Lipid -0.226 0.974 ***

n-Pentadecane Lipid -0.149 0.989 ***

n-Octadecane Lipid -0.277 0.961 NS

n-Eicosane Lipid -0.458 0.889 NS

n-Heptacosane (C27) Lipid 0.993 0.114 NS

28

n-Undecane Lipid -0.409 0.912 NS

n-Heptane Lipid 0.486 0.874 NS

3-Decene Lipid 0.561 -0.828 NS

n-Nonane Lipid -0.386 0.923 NS

C14_alkene_#3 Lipid 0.993 -0.116 NS

n-Dodecane Lipid -0.516 0.856 NS

n-Tetradecane Lipid -0.516 0.856 NS

n-Heneicosane (C21) Lipid -0.117 0.993 NS

n-Nonacosane (C29) Lipid -0.452 -0.892 NS

1,3-Butadiene Lipid -0.261 -0.965 NS

2-Propyn-1-amine Lipid -0.344 -0.939 NS

1-Butyne, 3,3-dimethyl- Lipid -0.143 0.990 NS

n-Docosane (C22) Lipid -0.053 0.999 NS

1-Pentene, 3-ethyl-2-methyl- Lipid 1.000 0.004 NS

n-Hentriacontane (C31) Lipid -0.348 -0.938 NS

n-Hexacosane (C26) Lipid 0.968 0.251 NS

n-Tricosane (C23) Lipid 0.968 0.251 NS

Dodecene Lipid 0.994 -0.112 NS

n-Octacosane Lipid -0.362 -0.932 NS

1,3-Octadiene Lipid -0.305 0.952 NS

29

1,4-Benzenediamine N-Bearing -0.074 0.997 †

2,5-Furandione, 3-methyl- N-Bearing -0.250 -0.968 *

1H-Pyrrole-2-carboxaldehyde, 1-methyl- N-Bearing 0.128 0.992 *

2-Propenenitrile, 2-methyl- N-Bearing -0.850 -0.527 *

Pyridine, 4-methoxy- N-Bearing 0.045 0.999 *

3-Pyridinol N-Bearing 0.458 0.889 *

3-Pyridinecarbonitrile N-Bearing -0.441 0.897 *

Alpha-amino-gamma-butyrolactone N-Bearing 0.818 0.575 *

5H-1-Pyrindine N-Bearing -0.770 -0.638 **

Pyridine 3-methyl N-Bearing -0.625 0.781 **

Pyridine, 2-ethyl N-Bearing -0.987 0.164 **

Propane, 2-nitro- N-Bearing -0.496 0.869 **

Acetamide, N-(2,4-dihydroxyphenyl)- N-Bearing -0.141 0.990 **

Ethanone, 1-(1-methyl-1H-pyrrol-2-yl)- N-Bearing -0.121 0.993 **

2-Pyridinealdehyde N-Bearing -0.084 0.996 **

2-(N-Methyl-N-ethylamino)phenol N-Bearing -0.213 0.977 **

Piperidine-2,5-dione N-Bearing -0.930 -0.368 **

4-Pyridinecarboxaldehyde N-Bearing -0.960 -0.281 ***

Aniline N-Bearing -0.838 0.545 ***

3-Phenylpyridine N-Bearing -0.322 0.947 ***

30

1H-Pyrrole, 3-methyl- N-Bearing -0.273 0.962 ***

Nicotinyl Alcohol N-Bearing -0.166 0.986 ***

1H-Pyrrole-2-carboxaldehyde N-Bearing -0.101 0.995 ***

N-Butyl-tert-butylamine N-Bearing 0.787 0.617 ***

2-Pyridinecarbonitrile N-Bearing -0.921 0.390 NS

Diethyltoluamide (DEET) N-Bearing -0.435 0.900 NS

4-Amino-2(1H)-pyridinone N-Bearing 0.844 0.536 NS

Acetamide, N-hydroxy N-Bearing 0.287 0.958 NS

N-Isopropyl-4-piperidone N-Bearing 0.724 0.690 NS

Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)- N-Bearing 0.873 0.488 NS

1H-1,2,4-Triazole N-Bearing -0.656 -0.755 NS


Pyrimidine N-Bearing 0.557 0.831 NS

Pyrazolo[5,1-c][1,2,4]benzotriazin-8-ol N-Bearing -0.145 0.989 NS

Pyrazine, 2-methoxy-3-(1-methylpropyl)- N-Bearing 0.979 -0.206 NS

1H-Tetrazole, 1-methyl- N-Bearing -0.817 0.577 NS

p-Aminotoluene N-Bearing -0.462 -0.887 NS

Hexanedinitrile N-Bearing -0.999 -0.040 NS

2-Amino-4-methylpyrimidine N-Bearing 0.845 -0.535 NS

4(1H)-Pyridinone, 2,3-dihydro-1-methyl- N-Bearing 0.946 -0.325 NS

31

Phenol, 4-methyl- Phenol -0.977 -0.212 ***

Phenol Phenol -0.308 0.951 ***

p-isopropenylphenol Phenol -0.499 -0.867 NS

Benzene, 1-ethyl-4-methoxy- Phenol 0.873 -0.487 *

2-Furanmethanol Polysaccharide 0.774 0.634 *

Benzofuran, 2-methyl- Polysaccharide -0.978 -0.207 *

Furan, 2,5-dimethyl- Polysaccharide -0.428 -0.904 **

Furan, 2-ethyl- Polysaccharide -0.928 -0.373 **

Furan, 2-ethyl-5-methyl- Polysaccharide -0.523 -0.852 **

Benzofuran, 2,3-dihydro- Polysaccharide 0.995 0.104 **

2-Acetylfuran Polysaccharide -0.704 -0.710 **

2-Cyclopenten-1-one, 2-hydroxy-3-methyl- Polysaccharide 0.671 0.741 **


2(3H)-Benzofuranone, 3-methyl- Polysaccharide -0.377 -0.926 ***

Furan, 2,3,5-trimethyl- Polysaccharide -0.443 -0.897 ***


Levoglucosenone Polysaccharide -0.311 0.950 ***

Furan, 2,4-dimethyl- Polysaccharide -0.257 0.966 ***

3-Acetamidofuran Polysaccharide -0.203 0.979 ***

1,2-Cyclopentanedione Polysaccharide -0.165 0.986 ***

32

3-Furaldehyde Polysaccharide -0.141 0.990 ***

Butanal, 2-methyl- Polysaccharide -0.105 0.994 ***

2(5H)-Furanone Polysaccharide -0.074 0.997 ***

Furfural Polysaccharide -0.069 0.998 ***

Acetic anhydride Polysaccharide 0.987 0.158 ***

2(5H)-Furanone, 5-methyl- Polysaccharide 0.089 0.996 NS

4H-Pyran-4-one, 3-hydroxy-2-methyl- Polysaccharide 0.312 0.950 NS

Levoglucosan Polysaccharide 0.313 0.950 NS

Furfural, 5-methyl- Polysaccharide -0.297 -0.955 NS

2-Cyclopenten-1-one, 2,3-dimethyl- Polysaccharide -0.815 -0.580 NS

PENTANAL Polysaccharide 0.907 -0.421 NS

Vinylfuran Polysaccharide -0.985 -0.171 NS

Cyclopent-2-ene-1-one, 2,3,4-trimethyl- Polysaccharide -0.948 -0.320 NS

Cyclopentanone Polysaccharide -0.464 0.886 NS

Acetic acid Polysaccharide 0.899 -0.438 NS

2H-Pyran-2-one Polysaccharide -0.201 0.980 NS

Methyl 2-furoate Polysaccharide 0.617 0.787 NS

2(3H)-Furanone, 5-methyl- Polysaccharide -0.890 -0.457 NS

1,2-Cyclohexanedione Polysaccharide 0.823 0.568 NS

5-Ethyl-2-furaldehyde Polysaccharide -0.358 -0.934 NS

33

Cyclopropanecarboxaldehyde, methylene- Polysaccharide -0.895 0.445 NS

1H-Pyrrole, 1-methyl- Protein 0.041 0.999 †

Benzonitrile Protein -0.991 -0.133 **

Ethylbenzene Protein -0.962 0.274 **

1 H-Pyrrole, 2-ethyl- Protein -0.585 0.811 **

Pyridine Protein -0.938 0.346 ***

Pyrrole Protein -0.927 0.376 ***

1H-Pyrrole, 2-methyl- Protein -0.898 0.440 ***

4-Pyridinamine Protein -0.857 0.516 ***

Indole Protein -0.507 0.862 ***

Benzyl nitrile Protein -0.442 0.897 ***

Benzenepropanenitrile Protein -0.266 0.964 ***

3-Methylindole Protein -0.186 0.983 ***

Pyridine, 3,5-dimethyl- Protein -0.696 -0.718 NS


Styrene Protein 1.000 0.004 NS

Resorcinol (Dihydroxybenzene) Unknown Origin 0.907 0.421 †

2,3,6-Trimethylnaphthalene Unknown Origin -0.795 0.607 †

Acenaphthene Unknown Origin -0.410 0.912 †

Fluoranthene Unknown Origin -0.469 0.883 †

34

1,3,5-Cyclooctatriene Unknown Origin -0.253 -0.968 †

2-Butanone, 3,3-dimethyl- Unknown Origin 0.560 0.829 †

C11_H12 Unknown Origin -0.709 0.705 †

1,2-Benzenediol, 3-methoxy- Unknown Origin 0.994 0.107 *

C9_H8 Unknown Origin -0.954 0.300 *

Benzene, 1,4-dimethoxy-2,3,5,6-tetramethyl- Unknown Origin 0.979 0.202 *

Phenol, 4-(1,1,3,3-tetramethylbutyl)- Unknown Origin -0.324 -0.946 *

D-Limonene Unknown Origin 0.999 0.034 *

Propanoic acid, 2-hydroxy-, methyl ester, (Ò)- Unknown Origin 0.632 0.775 *

1-Undecanol Unknown Origin -0.252 0.968 **

Pyruvaldehyde Unknown Origin 0.963 -0.269 **

1,3,5,7-Cyclooctatetraene Unknown Origin -0.880 0.475 **

Indane Unknown Origin -0.811 0.585 **

Dimethylbenzofuran Unknown Origin -0.649 -0.761 **

Thiophene Unknown Origin -0.532 -0.847 **

Benzene, heptyl- Unknown Origin -0.297 0.955 **

2-Propen-1-ol Unknown Origin -0.230 0.973 **

Phosphonic acid, (p-hydroxyphenyl)- Unknown Origin -0.278 -0.961 ***

3-Penten-2-one, (E)- Unknown Origin -0.367 -0.930 ***

3-Methylthiophene Unknown Origin -0.428 -0.904 ***

35

Toluene Unknown Origin -0.836 -0.549 ***

1H-Inden-1-one, 2,3-dihydro- Unknown Origin -0.999 -0.041 ***

Methanesulfonic acid, methyl ester Unknown Origin -0.711 0.703 ***

2,3,5-Trimethylnaphthalene Unknown Origin -0.392 0.920 ***

C8_H16 Unknown Origin -0.321 0.947 ***

2-Propenoic acid, ethenyl ester Unknown Origin -0.140 0.990 ***

1,3,5-Cycloheptatriene Unknown Origin -0.129 0.992 ***

Mequinol Unknown Origin 0.910 -0.414 ***

Ethanone, 1-(3-hydroxy-4-methoxyphenyl)- Unknown Origin 0.997 -0.079 ***


Unknown Origin 1.000 0.016 ***

2-Methylthiophene Unknown Origin -0.364 -0.931 NS

Pyrene Unknown Origin -0.403 0.915 NS

7-Methylindan-1-one Unknown Origin -0.920 -0.391 NS

Hydroquinone Unknown Origin -0.430 -0.903 NS

Phenol, 2-methoxy-5-(1-propenyl)-, (E)- Unknown Origin 0.999 -0.052 NS

2-Cyclohexen-1-one Unknown Origin -0.157 -0.988 NS

2-Methoxy-5-methylphenol Unknown Origin 0.996 0.089 NS

Beta-Pinene Unknown Origin 0.885 -0.466 NS

Trimethylphenol Unknown Origin 0.931 0.366 NS

2-Pentanone Unknown Origin -0.395 -0.919 NS

36

3-Methylthiophene-2-carbonitrile Unknown Origin -0.516 0.856 NS

Thiocyanic acid, methyl ester Unknown Origin -0.452 -0.892 NS

1-Propene, 3-azido- Unknown Origin -0.365 0.931 NS

Benzene, pentyl- Unknown Origin -0.365 0.931 NS

4-Hydroxy-2-methylacetophenone Unknown Origin 0.348 0.937 NS

Calamenene (Naphthalene) Unknown Origin -0.431 0.902 NS

Spiro[2.4]hepta-4,6-diene Unknown Origin -0.135 0.991 NS

Disulfide, dimethyl Unknown Origin 0.617 0.787 NS


2-Propenoic acid, anhydride Unknown Origin 0.946 -0.325 NS

37

Figure S1. Plots of significant correlations between the proportion of surface-derived C reaching the B horizon as determined from the DO13C mixing model against E horizon C stock (A), C:Alpy ratio (B), C:Fepy ratio (C), % imogolite-type minerals (D) and NMDS Axis 1 scores from our ordination of compounds identified in O-horizon DOM (E). Closed symbols represent data from red pine stands and open symbols represent data from sugar maple stands

translocation of carbon from surface organic horizons to ... file1 table s1. list of compounds...

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