transgenic groundnut for drought tolerance
TRANSCRIPT
Freedom to Operate (FTO)
A FTO study was conducted for Indian & US jurisdictions on various components including DREB1A, NOS Promoter, npt II Marker, rd29A Promoter, NOS Terminator.
It was observed from the preliminary FTO study that there is no granted Indian patent on these components exists till date and hence the research activity is not infringing any patent rights in India.
IMOD Inclusive Market Oriented Development Innovate • Grow • Prosper •
Timelines and Cost
R&D Event Selection BRL 1 BRL 2 Introgression
In-House PTTC & PartnersCurrent Stage
Event Approval
500,000438,667 410,000 206,000205,556
2013/14 2014/152011/12
Translational Research
FTO analysis conducted for Indian and US jurisdictions on various technology components indicated non-infringement of any patent rights
Product Development Cycle
A
A
B
Homology to Known allergens, toxins,
anti-nutrients Blast Searches
Heat Stability
Acute Oral Toxicity in Rats/Mice
(14 Days)
Pepsin Digestibility
Inheritance Over Multiple Generations
RT-PCR, Southern, qPCR data available as above
Western & ELISA to be performed with Ab (A)
produced from the protein
Pedigree Map
Documentation from the data recorded
from T1 to T5
Sequence data of inserted genetic material & of the flanking regions
Identification of ORFs
Genome Walking
Organisation of inserted genetic
material
PCR & Sequencing
Net House Evaluation
Field trials
Season 1
Field trials
Season 2
Exp. Level of Protein
ELISA
Reproductive & Survival Biology
Phenotyping
Site 1
Site 2
Site 3
Site 1
Site 2
Site 3
Compositional Analysis
Impact on Non-Target
Org.– Tier 1
Impact on Non-Target
Org – Tier 2
Livestock Feeding Studies
Number of Insertion Sites
qPCR data available as
above
Antibody Production
Protein Equivalence Study
Functional, Structural and Biochemical Equivalence
Plant Protein purification
Purification & scale up of protein from Bacterial / other
systems
PCR, Southern, qPCR, RT-PCR
PCR, Southern, qPCR, RT-PCR
PCR, Southern, qPCR, RT-PCR
PCR, Southern, qPCR, RT-PCR
PCR, Southern, qPCR, RT-PCR
Transformation T0 T1 T2 T3 T5T4 BConstruct
preparation
R&
DE
ven
t S
ele
ctio
nB
RL-I
BR
L-
II
Sub-Chronic Feeding Study in Rodents
(90 Days)
Molecular Studies Field Studies
Protein StudiesField trials
Gene Flow
Aggressiveness & Weediness
LOD 0.01%
Strictly Confidential
A
A
B
Homology to Known allergens, toxins,
anti-nutrients Blast Searches
Heat Stability
Acute Oral Toxicity in Rats/Mice
(14 Days)
Pepsin Digestibility
Inheritance Over Multiple Generations
RT-PCR, Southern, qPCR data available as above
Western & ELISA to be performed with Ab (A)
produced from the protein
Pedigree Map
Documentation from the data recorded
from T1 to T5
Sequence data of inserted genetic material & of the flanking regions
Identification of ORFs
Genome Walking
Organisation of inserted genetic
material
PCR & Sequencing
Net House Evaluation
Field trials
Season 1
Field trials
Season 2
Exp. Level of Protein
ELISA
Reproductive & Survival Biology
Phenotyping
Site 1
Site 2
Site 3
Site 1
Site 2
Site 3
Site 1
Site 2
Site 3
Site 1
Site 2
Site 3
Compositional Analysis
Impact on Non-Target
Org.– Tier 1
Impact on Non-Target
Org – Tier 2
Livestock Feeding Studies
Number of Insertion Sites
qPCR data available as
above
Antibody Production
Protein Equivalence Study
Functional, Structural and Biochemical Equivalence
Plant Protein purification
Purification & scale up of protein from Bacterial / other
systems
PCR, Southern, qPCR, RT-PCR
PCR, Southern, qPCR, RT-PCR
PCR, Southern, qPCR, RT-PCR
PCR, Southern, qPCR, RT-PCR
PCR, Southern, qPCR, RT-PCR
Transformation T0 T1 T2 T3 T5T4 BConstruct
preparation
R&
DE
ven
t S
ele
ctio
nB
RL-I
BR
L-
II
Sub-Chronic Feeding Study in Rodents
(90 Days)
Molecular Studies Field Studies
Protein StudiesField trials
Gene Flow
Aggressiveness & Weediness
LOD 0.01%
Strictly Confidential
Transgenic Groundnut for Drought ToleranceRoadmap for Product Development
A model depicting product development and dissemination following the regulatory approval for the selected event(s)
PTTC
ICRISAT
NARS / SAUs
KVKs / ARS
State Seed Corporation
Farmers
Non-Market Market
Technology Provider
Product Development & regulatory approvals
Varietal Development & Seed Production
Product Dissemination
PTTC
ICRISAT
NARS / SAUs
KVKs / ARS
State Seed Corporation
Farmers
Non-Market Market
PTTC
ICRISAT
NARS / SAUs
KVKs / ARS
State Seed Corporation
Farmers
Non-Market Market
Technology Provider
Product Development & regulatory approvals
Varietal Development & Seed Production
Product Dissemination
Preliminary Biosafety Data
Bioinformatics studies for potential allergenicity indicated absence of any homology of recombinant protein with known toxin / allergen
Overview
Drought
Signal Transduction
Transcription / Translation
Promoter APromoter A
Promoter BPromoter B
Promoter CPromoter C
Promoter DPromoter D
Signal PerceptionSignal Perception
Gene AGene A
Gene B Gene B
Gene CGene C
Gene DGene D
DRE
Plant Cell
Gene Expression
Stress response & tolerance
Regulatory Protein(Transcriptional Activator)
DREB1ADREB1Ard29A-Prd29A-P
Drought
Signal Transduction
Transcription / Translation
Promoter APromoter A
Promoter BPromoter B
Promoter CPromoter C
Promoter DPromoter D
Signal PerceptionSignal Perception
Promoter APromoter A
Promoter BPromoter B
Promoter CPromoter C
Promoter DPromoter D
Signal PerceptionSignal Perception
Gene AGene A
Gene B Gene B
Gene CGene C
Gene DGene D
Gene AGene A
Gene B Gene B
Gene CGene C
Gene DGene D
DRE
Plant Cell
Gene Expression
Stress response & tolerance
Regulatory Protein(Transcriptional Activator)
DREB1ADREB1Ard29A-Prd29A-PImproved drought
tolerance
• Improved seed and pod yield (upto 26%)
• Higher root dry weight and root length density at deeper soil (40-90cm)
• Improved drought tolerance indices (higher relative harvest index (RHI) and yield reduction under drought stress)
Sep 2011