trace tr-v1 gc columns
TRANSCRIPT
Index
i
Table of Contents
Column Selection
SPE Phase Selection ...........................................................2HPLC and LC/MS Column Selection ...................................4GC Column Selection ........................................................17
SPE
HyperSep SPE Products ....................................................30 Finnpipettes.......................................................................46
GC and GC/MS
TRACE GC Capillary Columns ...........................................48 Ultra Fast GC Columns......................................................65
HPLC and LC/MS
Column Protection.............................................................66Hypersil GOLD Columns....................................................70 BioBasic Columns..............................................................88 Hypercarb Columns .........................................................101 Hypersil BDS Columns ....................................................106 Hypersil Classical Columns.............................................111 Polymeric HPLC Columns ................................................122 AQUASIL C18 Columns ...................................................128 BetaBasic Columns .........................................................129 BetaMax Columns...........................................................130 BETASIL Columns ............................................................130 Other HPLC Columns.......................................................132
Accessories for HPLC and GC
HPLC Accessories............................................................136HPLC Injectors and Valves ..............................................144 GC Accessories ...............................................................150 Syringes...........................................................................161 Vials, Caps and Vial Accessories....................................169
Chromatography and Mass SpectrometryInstrument Parts and Supplies
GC and GC/MS................................................................176
Turboflow Columns..........................................................188
LC and LC/MS .................................................................189
MS ...............................................................................195
IRMS ...............................................................................198
ICP-MS ............................................................................199
Technical Information
SPE Method Development and Troubleshooting............204 HPLC Method Selection, Troubleshooting
and Reference Data...............................................207 GC Method Selection, Troubleshooting
and Reference Data...............................................221
Applications
Pharma/Biochem.............................................................230 Environmental .................................................................258 Forensics/Toxicology .......................................................276 Industrial .........................................................................288 Food Safety .....................................................................302
Indexes
Applications Index...........................................................318 Alphabetical Index ..........................................................330
317
229
203
175
135
47
65
29
1
Trademark Information
The Thermo Scientific name, the Thermo Scientific logo and the following trademarks are the property of Thermo Fisher Scientific Inc. and/or its subsidiaries:
Trademark Information
To order or for customer support, please see back cover.ii
3M CompanyKel-F
Agilent Technologies, Inc.AccuBOND, Agilent, DB, Evidex, HP-INNOWax, HP-Ultra, Zorbax
Applera CorporationPOROS
Becton, Dickinson and CompanyLuer-LOK
Bio-Rad Laboratories, Inc.Aminex
Biotage ABEVOLUTE, Isolute
Bristol-Myers Squibb CompanyTaxol
Capital Analytical, Ltd.RH
Chromatography Research Supplies, Inc.BTO
Cohesive Technologies, Inc.Aria
Dow Chemical Company, TheTRITON X-100
E.I. Dupont de Nemours and CompanyTeflon, Tefzel, Vespel, Viton
Eka Chemicals ABKromasil
Floridin CompanyFlorisil
General Electric CompanySE
GL Sciences, Inc.Inertsil
Hamilton CompanyHamilton
HighChem, Ltd.Mass Frontier
Hewlett-Packard Development CompanyHP
Kimberly-Clark CorporationKimwipes
Macherey-Nagel GmbHCHROMABOND, NUCLEODUR, Nucleosil, Optima
Mallinckrodt Baker, Inc.BAKERBOND spe, BAKERBOND, Polar Plus
Merck KGaALiChrosorb, LiChrospher, Purospher
Merlin Instrument CompanyMicroseal
Neos CorporationFluofix
New Objective, Inc.IntegraFrit, PicoFrit
Ohio Valley Specialty Chemical CompanyOV
Parker Hannifin CorporationParker
PerkinElmer, Inc.Clarus , PE
Pfizer, Inc.Dostinex
Phenomenex, Inc.AQUA, Columbus, Gemini, Jupiter, Luna, Prodigy,strata, Synergi, Ultracarb, Zebron ZB
Porvair plcPorvair
Quadrex Corporation007
Restek CorporationRtx, Stabilwax, Pinnacle, Viva
Rheodyne LLCMake-Before-Break (MBB), RheBuild, RheFlex,Rheodyne
Scientific Glass Technology Exploitatie B.V.CLICK-ON, Shortix, SGT, Super Clean
SGE, Inc.Auto-Sep T, Auto-Sep, BP, BPX, Enduro, SGE,PEEKsil, SilTite, Terry Tool
Shimadzu CorporationShimadzu
Showa Denko K.K.Asahipak, Shodex
Sigma-Aldrich CorporationDiscovery, Meridian MDN, Nukol, Petrocol, PTE, SP,SPB, SPME, Supelclean, Supelcosil, SUPELCOWAX,VOCOL
Tosoh CorporationTSKgel
Union Carbide CorporationCarbowax
United Chemical Technologies, Inc.Clean-Up
University of WashingtonSEQUEST, TurboSEQUEST
Upchurch Scientific, Inc.Bottom-of-the-Bottle, Upchurch Scientific
Varian, Inc.Bond Elut, Bond Elut Certify, CB, CC, CP-Select, CP-Sil, CP-Wax, Polaris, Pursuit, Varian
Velcro Industries B.V.Velcro
VICI, Valco Instruments Co., Inc.Cheminert, Valco
Victrex plc.VICTREX, PEEK
Waters CorporationµBondapak, ACQUITY UPLC, Atlantis, Nova-Pak,Sep-Pak, Styragel, SunFire, Symmetry, Ultrahydrogel,Ultrastyragel, Waters, µBondapak, Waters Spherisorb,Symmetry, SymmetryShield, XBridge, Xterra
Whatman, Inc.Partisil
W.R. Grace & CompanyAlltima, AT, Denali, Genesis, Vydac
YMC Co., Ltd.ODS-AQ, ODS-A, Pro-C18, YMCbasic, YMC-Pack,YMC
Other Notes:AquaSil™ Siliconizing Fluid for treating glass surfaces issold by Pierce Chemical Co., Rockford, IL
The following brands, trademarks or service marks are the property of the listed company and/or its subsidiaries.Every effort has been taken to ensure this list is accurate at the time of printing this catalog.
BetaBasic
BetaMax
BETASIL
BioBasic
BioMate
BioWorks
ChromQuest
COOL POCKET
DASH
Deca
DELTABOND
Discovery
DSQ
Dual Desolvation Zone
Evolution
Fingerlok
Finnigan
Fluophase
FocusLiner
FOCUS
HOT POCKET
Hypercarb
HyperGEL
Hypersil
Hypersil GOLD
Hypersil GOLD aQ
HyperPrep
HyperQuad
HyperREZ
HyperSep
HyPURITY
HyPURITY ADVANCE
HyPURITY AQUASTAR
Ion Bright
Javelin
KAPPA
LCQ
LCQ Advantage
LightPipe
LTQ
LTQ FT
LXQ
MSQ
Open Access
PRISM
ProteomeX
Retain
SLIPFREE
Smart Accessories
SpectraSYSTEM
Spectronic
Surveyor
Surveyor MSQ Plus
Surveyor Plus
TRACE
TRACE GC Ultra
Transcend
TriPlus
TSQ Quantum
TSQ Quantum Ultra
UNIGUARD
Verify
Watson LIMS
Xcalibur
GC and GC/MS
GC and GC/MSThermo Scientific TRACE™ GC Columns offer high
temperature stability and exhibit low bleed and long
lifetimes. From general purpose non-polar to polar
columns, many of which are MS-compatible, TRACE
GC columns provide excellent quality and performance,
with guaranteed reproducibility.
Thermo Scientific TRACE GC Capillary ColumnsA leap forward in column performance
• Low bleed even at elevated temperatures
• Outstanding robustness for difficultseparations
• Reliable and reproducible performance• Complete range of GC consumables
for all instruments
The heart of any gas chromatograph is thecolumn. We have used the knowledgegained over 30 years of HPLC column production and 50 years in GC and GC/MSinstrument manufacturing to offer a range of columns and consumables that are unsurpassed in the most important aspects of their performance.
Low BleedBleeding phases cause problems includinglow sensitivity, detector contamination, lowertemperature limits and short lifetimes. Allmanufacturers have at some time claimedtheir columns have the lowest bleed, but usesupporting chromatograms that lack repro-ducible scale information or methodology. Byquantifying the amount of phase bleeding fromthe column, we can show that the ThermoScientific TRACE™ column range has lessthan half the bleed of other popular columns.
RobustnessMoisture and oxygen pose a danger to GCcolumns. Oxygen contamination can comefrom many sources, leading to shortenedlifetimes, increased bleed and increased cost.TRACE GC columns have been manufacturedwith improved phase bonding to minimizethe risk of damage due to contaminated carrier gas or difficult samples as shown in the two figures above.
ReproducibilityAs well as focusing on individual columnperformance, we work to improve column-to-column reproducibility, arising from majorimprovements in the production processes. A column bought today will have the samecharacteristics or better as one obtained ayear or ten years from now.
Guaranteed PerformanceAll TRACE columns have passed rigoroustesting procedures to ensure the highest possible performance and are delivered with aserialized certificate to show their performanceand assist with traceability. All ThermoScientific columns come with a 100% guar-antee. If for any reason the column does notperform up to our claims, we will replace itwith one that does.
Technical SupportOur quality technical support will help yousolve your problems quickly, via phone, ourweb resource center or the technical sectionof this catalog. Please refer to the GC ColumnSelection Guide on page 17 for informationto help you select the appropriate column foryour separation. If you are looking for a column for a NIOSH, ASTM or EPA method,Thermo Scientific column recommendationsare given on pages 21-27. Should you needassistance with column selection or methoddevelopment, please contact one of our tech-nical support desks and our highly trainedteam of scientists will be able to help.
Quick Reference IconThe reference graphic (below) on each column page gives easy access to the specifications of each phase type. It gives arelative measure of polarity, its chemistryand its maximum temperature limits as wellas its USP classification.
TRACE GC Columns
To order or for customer support, please see back cover.48
Phase: 100% Dimethyl Polysiloxane
Max Temps: 340 °C/360 °C for films <_ 1.0 µm300 °C/320 °C for 3.0 µm films280 °C/300 °C for films > 3.0 µm
USP Listing: G1, G2, G38
Example of TR-WaxMS robustness. Top: New column.
The stability of a TR-5MS column after three daysat 200 °C using air as a carrier gas. Very littlechange in the chromatography is evident belowthe column’s maximum operating temperature.
Bottom: Column performance after being heated to 250 °C for 1 hour with no carriergas. Although a loss of retention and sensitivity can be seen, the column showsextraordinary robustness after this treatment.
Phase: 100% Dimethyl Polysiloxane Max Temps: 340 °C/360 °C for films <_ 1.0 µm
300 °C/320 °C for 3.0 µm films280 °C/300 °C for films > 3.0 µm
USP Listing: G1, G2, G38
TRACE TR-1 GC ColumnsGeneral purpose non-polar column
• Low bleed for increased sensitivity• High operating temperature for easy reconditioning• 100% polydimethylsiloxane
The Thermo Scientific TRACE™ TR-1 GC columnis a general purpose, non-polar column for awide range of compounds and applications.The TRACE TR-1, an industry standard, has a low phase bleed and a maximum operatingtemperature of 360 °C. The high operatingtemperature makes it easy to bake out andrecondition the column in the event of columncontamination. The TR-1 is suitable for a rangeof detectors including mass spectrometry,and is solvent washable.
ApplicationsThe TRACE TR-1 is suitable for a very widerange of applications including hydrocarbons,PONA, esters, pesticides, drugs and amines.
Similar to:DB™-1, DB-Petro, BP™1, HP™-1, HP-1MS, Rtx™-1, Ultra-1, SPB™-1, SPB-1 Sulfur,Petrocol DH, CP-Sil™ 5CB, RSL-150, RSL-160,ZB™-1, CB™-1, OV-1, PE™-1, 007-1(MS), SP™-2100, SE-30, RH-1, CC™-1, CP-Sil 5CB MS,VF-1ms
See also Liners page 153.
GC and GC/MS
www.thermo.com /columns 49
Ordering InformationID (mm) Film Thickness (µm) Length (m) Part Number
0.10 0.10 10 260A020P
0.15 0.50 50 260A227P
0.25 0.25 15 260A130P
0.25 0.25 30 260A142P
0.25 0.25 60 260A154P
0.25 0.50 30 260A223P
0.25 0.50 100 260A241P
0.25 1.00 30 260A296P
0.25 1.00 60 260A308P
0.32 0.25 15 260A131P
0.32 0.25 30 260A143P
0.32 0.25 60 260A155P
0.32 0.50 30 260A224P
0.32 5.00 60 260A481P
0.32 1.00 30 260A297P
0.32 1.00 60 260A309P
0.32 3.00 30 260A395P
0.53 0.50 15 260A213P
0.53 0.50 30 260A225P
0.53 3.00 30 260A396P
0.53 5.00 30 260A470P
Analysis of phtalate esters on TRACE TR-1 column
TR-1, 15 m x 0.25 mm x 0.25 µmPart Number: 260A130PPhase: TR-1, 0.25 µm filmInitial Temp: 200 °C, 0 minRate: 20 °C/minFinal Temp: 280 °C, 2 minDetector: FIDSensitivity: 128 x 10 - 12 AFSInjection Mode: Split
Components1. Dimethyl Phthalate2. Diethyl Phthalate3. Di-iso-Propyl Phthalat4. Di-n-Propyl Phthalate5. Di-iso-Butyl Phthalate6. Di-n-Butyl Phthalate7. Di-n-Pentyl Phthalate8. Dihexyl Phthalate9. Diheptyl Phthalate10. Dioctyl Phthalate
TRACE TR-1MS GC ColumnsExtremely low bleed non-polar column
• High operating temperature• Inert phase suited for environmental analyses• Extremely low bleed phase
With an extremely low bleed and a maximumoperating temperature of 380 °C, the ThermoScientific TRACE™ TR-1MS column is ideal for all GC/MS applications. The column is particularly suited for applications such asGC/MS environmental analyses which requirea high temperature phase that is also inert.
ApplicationsThe TRACE TR-1MS is suitable for the analysis of chlorinated and nitroaromaticcompounds and other GC/MS applications.The inertness and high temperature capabilityof the column are features which are wellsuited to GC/MS environmental analyses.
Similar to:DB-1, DB-Petro, BP1, HP-1, HP-1MS, Rtx-1,Ultra-1, SPB-1, SPB-1 Sulfur, Petrocol DH,CP-Sil 5CB, RSL-150, RSL-160, ZB-1, CB-1,OV-1, PE-1, 007-1(MS), SP-2100, SE-30, RH™-1, CC-1, CP-Sil™ 5CB MS, VF-1ms
See also Gas Filters on pages 150-151.
Phase: 100% Dimethyl PolysiloxaneMax Temps: 370 °C/380 °CUSP Listing: G1, G2, G38
TRACE GC Columns
To order or for customer support, please see back cover.50
Analysis of polynuclear aromatic hydrocarbons (PAH)
Components1. Naphthalene2. Acenaphthylene3. Acenaphthene4. Fluorene5. Phenanthrene6. Anthracene7. Fluoranthene8. Pyrene9. Benzo(a)anthracene10. Chrysene11. Benzo(b)fluoranthene12. Benzo(k)fluoranthene13. Benzo(a)pyrene14. Indeno(1,2,3-c,d)pyrene15. Dibenzo(a,h)anthracene16. Benzo(g,h,i)perylene
TR1-MS, 30 m x 0.25 mm x 0.25 µm filmPart Number: 260B142PSample: 100 ppm in
DichloromethaneInitial Temp: 65 °C, 1 min.Rate 1: 25 °C/min to 140 °C,Rate 2: 10 °C/min to 240 °CRate 3: 5 °C/min to 300 °CFinal Temp: 300 °C, 2 min.Detector Type: Mass SpecCarrier Gas: He, 27.8 psiCarrier Gas Flow: 1.7 mL/minConstant Flow: On
Average Linear Velocity: 35 cm/sec at 65 °CInjection Mode: SplitSplit Ratio: 50:1Injection Volume: 1 µLInjection Temperature: 250 °CAutosampler: NoLiner Type: 4 mm ID Single
Taper LinerLiner Part Number: 453A1345Full Scan/SIM: Full scan 40-500
Ordering InformationID (mm) Film Thickness (µm) Length (m) Part Number
0.25 0.25 15 260B130P
0.25 0.25 30 260B142P
0.25 0.25 60 260B154P
0.32 0.25 30 260B143P
0.32 0.25 60 260B155P
Phase: 5% Phenyl Methylpolysiloxane (TR-5)
5% Phenyl Polycarborane Siloxane (TR-5HT)
Max Temps: 340 °C/350 °C for films <_ 1.5 µm280 °C/300 °C for films > 1.5 µm380 °C/400 °C for TR-5HT columns
USP Listing: G27, G36
TRACE TR-5 and TR-5HT GC ColumnsThe most widely used phase in gas chromatography
• High operating temperature• Ideal start for method development• Wide variety of applications
The TRACE™ TR-5 column is a 5% phenyl column with a slight increase in polaritycompared to the 100% methyl columns, TR-1and TR-1MS. The TRACE TR-5 is a universalcolumn with a wide application range, anextremely low bleed and maximum operatingtemperature of 350 °C. It is an ideal startingcolumn for method development and is capableof performing most required separations.
The TRACE TR-5HT columns extend theupper temperature limits as high as 400 °Callowing the elution of higher boilingHydrocarbons up to C100.
ApplicationsThe TRACE TR-5 and TR-5HT are suitable forapplications such as alcohols, free fatty acids,aromatics, flavors and low polarity pesticides.The universal use of the TR-5 column is illustrated by the separation of substitutedaromatics. The chromatogram shows excellentpeak shape of all components and separa-tion is based on boiling points.
Similar to:DB-5, BP5, Rtx-5, HP-5, Ultra-2, PTE™-5,SPB5, MDN™-5, CP-Sil 8CB, SPB-5, AT™-5,ZB-5, 007-2(MPS-5), SE-52, SE-54
See also Liners on page 153.
Ordering InformationPhase ID (mm) Film Thickness (µm) Length (m) Part Number
TR-5 0.25 0.25 15 260E130P
TR-5 0.25 0.25 30 260E142P
TR-5 0.25 0.25 60 260E154P
TR-5 0.25 0.50 30 260E223P
TR-5 0.25 1.00 30 260E296P
TR-5 0.25 1.00 60 260E308P
TR-5 0.32 0.25 7 260E113P
TR-5 0.32 0.25 15 260E131P
TR-5 0.32 0.25 30 260E143P
TR-5 0.32 0.25 60 260E155P
TR-5 0.32 0.50 30 260E224P
TR-5 0.32 1.00 30 260E297P
TR-5 0.32 1.00 60 260E309P
TR-5 0.53 0.50 30 260E225P
TR-5 0.53 1.00 15 260E286P
TR-5 0.53 1.00 30 260E298P
TR-5 0.53 1.50 15 260E334P
TR-5 0.53 1.50 30 260E336P
TR-5 0.53 5.00 30 260E470P
TR-5HT 0.32 0.1 12 260H030P
TR-5HT 0.25 0.1 30 260H047P
GC and GC/MS
www.thermo.com /columns 51
Analysis of substituted aromatics
TR-5, 30 m x 0.25 mm x 0.25 µmPart Number: 260E142PInitial Temp: 40 °C, 4 minRate 1: 2 °C/minTemp 2: 60 °CRate 2: 5 °C/minFinal Temp: 80 °C, 6 minDetector: FIDInjection Mode: Split
Components1. Benzene2. Toluene3. Chlorobenzene4. Ethylbenzene5. m,p-Xylene6. o-Xylene7. Styrene8. iso-Propylbenzene9. n-Propylbenzene10. 1,3,5-Trimethyl-benzene11. 1,2,4-Trimethyl-benzene12. m-Dichlorobenzene13. sec-Butylbenzene, p-Dichlorobenzene14. o-Dichlorobenzene15. Butyl-benzene16. 1,2,4-Trichloro-benzene17. Napthalene18. Hexachlorobutadiene
TRACE TR-5MS GC ColumnsThe most popular MS phase in gas chromatography
• Silphenylene phase• Lowest bleed, highest stability• High signal-to-noise increases sensitivity• High robustness to oxygen and water contamination
The Thermo Scientific TRACE™ TR-5MS columnis a silphenylene-based phase ideal for mostGC/MS applications. This low bleed columnis especially important for mass spectrometrybecause contamination of source componentswill quickly lead to poor results and costlyinstrument downtime. The TR-5MS column isslightly more polar compared to the TR-1columns, and operates up to a maximumtemperature of 370 °C.
The TR-5MS column is a universal columnwith a wide range of applications from envi-ronmental semi-volatile methods to food andfragrance applications. The column is veryinert (no active sites) thus reducing breakdownof labile compounds such as DDT and Endrin.The inertness of the column also results inminimal peak tailing. This, together with high efficiency, results in narrow peaks andexcellent separation based on boiling points.If the GC/MS is exposed to many variedmethods, then this is the preferred columnfor the instrument.
To demonstrate the robustness of the TR-5MS column, a new column was subjectedto 80 hours at 200 °C using air as a carrier gas.A comparison of its performance before andafter shows little change below its maximumoperating temperature (see page 48).
ApplicationsThe TRACE TR-5MS is ideal for all GC/MSapplications, particularly if the column isrequired for many different methods.
Similar to:DB-5, DB-5MS, DB-5.625 XTI-5, BPX5, Rtx-5MS, Rtx- 5, SilMS, AT-5, AT-5MS, 007-5MS, SPB-5, CP-Sil 8CB, Ultra-2, HP-5,HP-5MS, HP5-TA, SPB-5, MDN-5S, VF-5ms,RSL-200, CB-5, OV™-5, PE-5, 007-2(MP-5),SE-52, SE-54, PTE-5, CC-5, RH-5ms, ZB-5
See also Syringes on page 163.
Phase: 5% Phenyl Polysilphenylene-siloxaneMax Temps: 360 °C/370 °C for films <_ 1.5 µm
350 °C/360 °C for films > 1.5 µmUSP Listing: G27, G36
Ordering InformationID (mm) Film Thickness (µm) Length (m) Part Number
0.10 0.10 10 260F020P
0.10 0.10 20 260F145P
0.15 0.25 25 260F134P
0.18 0.18 20 260F578P
0.25 0.10 15 260F035P
0.25 0.10 30 260F047P
0.25 0.25 15 260F130P
0.25 0.25 30 260F142P
0.25 0.25 60 260F154P
0.25 0.50 30 260F223P
0.25 1.00 15 260F284P
0.25 1.00 30 260F296P
0.25 1.00 60 260F308P
0.32 0.25 15 260F131P
0.32 0.25 30 260F143P
0.32 0.25 60 260F155P
0.32 0.50 30 260F224P
0.32 1.00 15 260F285P
0.32 1.00 30 260F297P
0.32 1.00 60 260F309P
0.53 0.50 15 260F213P
0.53 0.50 30 260F225P
0.53 1.00 30 260F298P
0.53 1.50 30 260F336P
0.53 3.00 15 260F384P
0.53 3.00 30 260F396P
TRACE GC Columns
To order or for customer support, please see back cover.52
Basic drug screen on a TR-5MS (10 - 20 ng/component)
TR-5MS, 30 m x 0.53 mm x 1.0 µmPart Number: 260F298PInitial Temp: 120 °CRate: 10 °C/minFinal Temp: 310 °CDetector: FIDInjector: Split, 240 °CCarrier Gas: Hydrogen, 2 psi
Notes: The low bleed nature of the TR-5MS allows trace analysis to be performed
1. Methamphetamine2. Phendimetrazine3. Phencyclidine4. Mepivocaine5. Methaqualone
6. Amitriptyline7. Codeine8. Diazepam9. Fentanyl
Components (10 - 20 ng)
Phase: 35% Phenyl Polysilphenylene-siloxane
Max Temps: 360 °C/370 °C USP Listing: G42
TRACE TR-35MS GC ColumnsMid-polarity column ideal for many applications
• Exceptionally low surface activity• Suitable for pesticide analysis• Pharmaceutical confirmational column
The Thermo Scientific TRACE™ TR-35MS is asilphenylene-based phase ideal for GC/MSapplications, particularly applications requiringa high temperature phase and more polaritythan a 5% phenyl column. The TR-35MSphase has extremely low bleed, especiallyimportant in limiting contamination of sourcecomponents that can degrade results andcause costly instrument downtime. This alsoleads to a high signal-to-noise ratio, improvingyour sensitivity. The inertness of the columnphase and its high efficiency results in minimalpeak tailing, narrow peaks and excellentseparation. The TR-35MS column operatesup to a maximum temperature of 370 °C.
ApplicationsThe TR-35MS column is a universal, mid-polarity column used in a wide range ofapplications from environmental semi-volatilemethods to food and fragrance applications.
Similar to:DB-35, DB-35MS, HP-35, HP-35MS, MDN-35, Rtx-35, SPB-35, BPX35
See also Gas Filters on pages 150-151.
Ordering InformationID (mm) Film Thickness (µm) Length (m) Part Number
0.22 0.25 15 260C129P
0.25 0.25 15 260C130P
0.25 0.25 30 260C142P
0.25 0.25 60 260C154P
0.32 0.25 30 260C143P
0.32 0.25 60 260C155P
0.25 0.50 30 260C223P
0.32 0.50 30 260C224P
0.53 0.50 30 260C225P
0.53 1.00 15 260C286P
0.53 1.00 30 260C298P
GC and GC/MS
www.thermo.com /columns 53
Analysis of aromatic amines from diazo dyes
TR-35MS, 30 m x 0.25 mm x 0.25 µmPart Number: 260C142PAzo Dyes standard: 10 ppm solution in DCMInitial Temp: 50 °C, 2 minRate 1: 15 °C to 240 °CRate 2: 10 °C to 280 °CFinal Temp: 280 °C, 25 minDetector Type: Mass SpecCarrier Gas: Helium, 7.1 psiCarrier Gas Flow: 1.0 mL/minConstant Flow: OnAverage Linear Velocity: 36 cm/sec at 50 °CInjection Mode: SplitlessSplitless Time: 1.0 minSplitflow: 60 mL/minInjection Volume: 1 µLInjection Temperature: 250 °CAutosampler: NoLiner Type: 4 mm ID Double Taper Liner
Components1. Indolin2. o-Toluidine3. 2,4-Diaminoanisole4. p-Chloroaniline5. p-Cresidine6. 2,4,6-Trimethylaniline7. 4-Chlorotoluidine8. Unknown9. 2-Naphthylamine10. Unknown11. 4-Aminodiphenyl12. 4,4'-Ox ydianiline13. 4,4'-Diaminodiphenylmethane14. Benzidine15. 3,3'-Dimethyl-4,4'-diaminodiphenylm ethane16. 3,3'-dimethylbenzidine17. 4,4'-Thiodianiline18. 3,3'-Dichlorobenzidine19. 4,4'-Methylene-bis(2-chloroaniline)20. 3,3'-Dimethoxybenzidine
TRACE TR-1701 GC ColumnsMid-polarity column with alternative selectivity
• Low bleed• Ideal for pesticide analysis• Low surface activity
The Thermo Scientific TRACE™ TR-1701 columnis a popular mid-polarity column that sitsbetween the TR-50MS and the TR-35MS onthe polarity scale. It displays low bleed andhigh temperature stability compared with othersimilar columns. The TR-1701 column’s cyanoand phenyl groups increase polarity of thecolumn while offering alternative selectivity.The alternate selectivity of the TR-1701 columnwhen compared to a column of differentpolarity is particularly useful for the analysisof pesticides and other applications where achange in elution order is required for confir-mation purposes.
ApplicationsThe mid-polarity TRACE TR-1701 is particularlyuseful for the analysis of pesticides, PCBs,PAHs, organic acids, drugs and steroids.
Similar to:DB-1701, Rtx-1701, HP-1701, BP10, OV-1701,007-1701, CP-Sil 19 CB
See also SPE on page 30.
Phase: 14% CyanopropylphenylPolysiloxane
Max Temps: 280/300 °CUSP Listing: G46
Ordering InformationID (mm) Film Thickness (µm) Length (m) Part Number
0.25 0.25 15 260Q130P
0.25 0.25 30 260Q142P
0.25 0.25 60 260Q154P
0.32 0.25 15 260Q131P
0.32 0.25 30 260Q143P
0.32 0.25 60 260Q155P
0.32 0.50 30 260Q224P
0.53 1.00 30 260Q298P
TRACE GC Columns
To order or for customer support, please see back cover.54
Analysis of organochlorine pesticides
TR-1701, 30 m x 0.25 mm x 0.25 µmPart Number: 260Q142PInjector Temp: 240 °CInjection Volume: 1 µLInjection Type: Splitless, 0.5 minPurge On (Split) Vent: 60 mL/minLiner Type: Single taperCarrier Gas: HeliumConstant Flow: OnPressure: 14.1 psiColumn Flow: 1.3 mL/minLinear Velocity: 30 cm/sec at 40 °CInitial Temp: 40 °C, 1 minRate 1: 30 °C/minFinal Temp 1: 190 °C, 3 minRate 2: 10 °C/minFinal Temp 2: 300 °C, 5 minDetector: FID, 310 °C
Components1. 2,4,5,6-Tetrachloro-m-xylene (IS)2. α-BHC3. β-BHC4. γ-BHC5. δ-BHC6. Heptachlor7. Aldrin8. Heptachlorepoxide9. γ-Chlordane10. α-Chlordane11. Endosulfan A12. p,p'-DDE13. Dieldrin14. Endrin15. p,p'-DDD16. Endosulfan B17. Endrin Aldehyde18. p,p'-DDT19. Endosulfan Sulphate20. Methoxychlor21. Endrin Ketone22. Decachlorobiphenyl (IS)
Phase: 50% Phenyl Polysilphenylene-siloxane
Max Temps: 360 °C/370 °C USP Listing: G3
TRACE TR-50MS GC ColumnsMid-polarity GC column
• Silphenylene phase• Low bleed decreases MS contamination• Pesticide and confirmational column
The Thermo Scientific TRACE™ TR-50MS col-umn is a silphenylene-based phase ideal forall GC/MS applications. A low bleed columnis especially important for GC/MS as con-tamination of source components will quicklylead to a degradation of results and costlyinstrument downtime. This phase is extremelylow bleed with a maximum operating temper-ature of 370 °C. The inertness of the columnalso results in minimal peak tailing anddecreased breakdown of sensitive samples.This together with high efficiency results innarrow peaks and excellent separation.
ApplicationsThe TR-50MS column is particularly suitedfor applications that require a higher temper-ature and more polarity than a 5% phenylcolumn. The TR-50MS is often used in combination with a 5% phenyl column, suchas the TR-5MS, to help confirm the identityof unknown analytes.
Similar to:OV-17, SP-2250, DB-17, DB-17ms, DB-17ht,BPX50, Rtx-50, SPB-50, HP-50+, HP-17, AT50,RSL-300, PE-17, CC-17, 007-17(MPS-50),SPB-17, ZB-50
See also Septa on page 159.
Ordering InformationID (mm) Film Thickness (µm) Length (m) Part Number
0.10 0.10 10 260R020P
0.25 0.15 30 260R050P
0.25 0.25 15 260R130P
0.25 0.25 30 260R142P
0.25 0.25 60 260R154P
0.32 0.25 15 260R131P
0.32 0.25 30 260R143P
0.32 0.25 60 260R155P
0.53 0.50 15 260R213P
0.53 0.50 30 260R225P
GC and GC/MS
www.thermo.com /columns 55
Analysis of chlorine, phosphorus and nitrogen-based pesticides
TR-50MS, 30 m x 0.25 mm x 0.25 µmPart Number: 260R142PInitial Temp: 50 °C, 1 minRate 1: 4 °C/minFinal Temp: 280 °C, 1.5 minDetector: Mass SpecInjector Mode: Splitless, 0.50 minCarrier Gas: Helium, 33 cm/secInjection Volume: 0.5 µL
Components1. DDVP2. Etridiazole3. Chloroneb4. Molinate5. BPMC6. Benfluralin7. Pencycurone8. Simazin9. Diazinon-ox10. Propyzamide11. Diazinon12. TPN13. IBP14. ECP15. MBPMC16. Bromobutide
17. MEP-ox18. Tolclophos-Methyl19. Simetryn20. MEP21. Esprocarb22. Malathion23. Probenazole24. Chloropyrifos25. Thiobancarb26. Fthalide27. Pendimetaline28. Isofenfos29. Methyl-Dymron30. Captan31. Butamifos32. Napropamide
33. Flutolurin34. Prethirachlor35. Isoxathon-ox36. Isoprothiolate37. Tricyclazole38. Buprofezine39. Isoxathion40. Mepronil41. Chloronitrofen42. EDDP43. EPN-ox44. Pyridaphenthion45. Iprodione46. EPN47. Bifenox48. Mefenacet
TRACE TR-WAX GC ColumnsGeneral purpose high polarity column
• Polyethylene glycol phase for polar compounds• Cross-linked • Solvent washable
The Thermo Scientific TRACE™ TR-WAX is apolyethylene glycol-based phase ideal for theanalysis of all polar compounds. The phaseis highly cross-linked and fully deactivated,providing excellent peak shape, even withshort chain alcohols.
ApplicationThe TRACE TR-WAX is ideal for polar com-pound analysis, particularly esters, alcohols,ketones, glycols and aromatic isomers.
Similar to:DB-Wax, BP20, Rtx-Wax, Stabilwax, HP20M,HP-Wax, HP-INNOWax™, Supelcowax-10, AT-Wax, Nukol, CP Wax™52CB, SUPEROX II, Carbowax™, PE™-WAX, ZBWAX
See also Retention Gaps on page 152.
Phase: Polyethylene glycol (PEG) Max Temps: 260 °C/280 °C for films < 1.0 µm
240 °C/260 °C for 1.0 µmUSP Listing: G16, G20
Ordering InformationID (mm) Film Thickness (µm) Length (m) Part Number
0.10 0.10 10 260W020P
0.25 0.25 15 260W130P
0.25 0.25 30 260W142P
0.25 0.25 60 260W154P
0.25 0.50 30 260W223P
0.25 0.50 60 260W235P
0.25 1.00 30 260W296P
0.32 0.25 15 260W131P
0.32 0.25 30 260W143P
0.32 0.25 60 260W155P
0.32 0.50 30 260W224P
0.32 0.50 60 260W236P
0.32 1.00 30 260W297P
0.32 1.00 60 260W309P
0.53 0.50 30 260W225P
0.53 1.00 15 260W286P
0.53 1.00 30 260W298P
0.53 1.00 60 260W310P
TRACE GC Columns
To order or for customer support, please see back cover.56
Analysis of 18 alcohols
TR-WAX, 30 m x 0.25 mm x 0.25 µmPart Number: 260W142PInitial Temp: 45 °C, 2 minRate: 3 °C/minFinal Temp: 80 °C, 0 minDetector: FIDInjection Mode: Split
Components1. Methanol2. Propan-2-ol3. Ethanol4. sec-Butan-1-ol5. n-Propanol+2-Methyl-3-Buten-2-ol6. d,l-3-Methyl-2-Butan-1-ol7. Pentan-3-ol8. d,l-2-Pentan-1-ol9. n-Butanol10. 2,4-Dimethyl, Pentan-3-ol11. Hexan-3-ol12. 2-Methyl Prop-2-en-1-ol13. Crotyl Alcohol (2-Buten-1-ol)14. Hexan-2-ol15. Pentan-1-ol16. 2-Methyl, Pentan-1-ol17. 2-Ethyl Butan-1-ol18. Hexan-1-ol
Phase: Polyethylene Glycol Max Temps: 280/300 °C USP Listing: G16
TRACE TR-WaxMS GC ColumnsHigh polarity phase designed for mass spectrometry detectors
• Proprietary bonding method expands operating temperatures• Extremely low bleed improves sensitivity• High stability with oxygen and water
The Thermo Scientific TRACE™ TR-WaxMS GCcolumn is a low bleed, highly stable polar GC column. The TRACE TR-WaxMS columnuses a proprietary bonding technique thatproduces a column with significantly lowerbleed levels and extended maximum allowableoperating temperature (280 °C) than otherhigh polarity columns available. The low bleedcharacteristics of this column make it espe-cially suitable for use with a mass spectro-metric detector, resulting in higher sensitivity,better library matches and less instrumentcleaning. The column is strongly resistant towater, making the TR-WaxMS one of themost versatile, sensitive, stable and longlifetime PEG phase columns on the market.Please see page 48 for demonstration of theTR-WaxMS stability under adverse treatments.
ApplicationsThe TRACE TR-WaxMS is ideally suited tothe analysis of aromatic hydrocarbons, foodadditives, essential oils, alcohols, esters,aldehydes and keytones. The highly stable,high polarity TR-WaxMS column is suitablefor all PEG analyses, with low bleed toenhance sensitivity.
Similar to:DB-Wax, Rtx-Wax, Stabilwax™, HP20M, BP20,HP-Wax, HP-INNOWax, SUPELCOWAX 10,AT-Wax, Nukol™, CP Wax 52CB, ZB-Wax
See also Siltite Ferrules on page 156.
Ordering InformationID (mm) Film Thickness (µm) Length (m) Part Number
0.25 0.25 30 260X142P
0.25 0.25 60 260X154P
0.25 1.00 30 260X296P
0.32 0.25 30 260X143P
0.32 0.25 60 260X155P
0.32 0.50 30 260X224P
0.53 1.00 30 260X298P
1. p-Methyl anisole2. α-Copaene3. Linalool L4. β-Caryophyllene5. Methyl benzoate6. α-Humulene7. α-Amorphene8. Germacrene9. Benzyl acetate10. δ-Cadinene11. α-Farnesene12. Geranyl acetate13. trans-Cinamyl acetate14. Farnesyl acetate15. Farnesol16. Benzyl benzoate17. Benzyl salicylate
TR-WaxMS, 30 m x 0.25 mm x 0.25 µmPart Number: 260X142PSample: Ylang Ylang oil (neat)Injection Temp: 250 °CInjection Mode: SplitSplit Ratio: 120:1Injection Volume: 0.1 µLInitial Temp: 40 °C, 2 minRate 1: 3 °C/min to 260 °C,Final Temp: 260 °C, 10 minCarrier Gas: helium, 25.7 psiCarrier Gas Flow: 1.8 mL/minConstant Flow: OnAverage Linear Velocity: 35 cm/sec at 40 °CDetector Type: FID
Analysis of ylang ylang oil
GC and GC/MS
www.thermo.com /columns 57
TRACE TR-SimDist GC ColumnsLow polarity phase for high temperature analysis
• Optimized for simulated distillation analysis• High temperature limits• Reproducible low bleed
The Thermo Scientific TRACE™ TR-SimDist column is a strongly cross-linked, high temperature column for use in simulated distillation analysis. The TR-SimDist columnmeets the demanding requirements of simu-lated distillation analysis with a temperaturelimit of 400 °C. The phase is strongly cross-linked, and the stable low bleed profile alsoallows for consistent background subtractionwhich is common practice.
ApplicationsThe TRACE TR-SimDist is suited for simulateddistillation analysis using high temperaturesto elute high molecular weight hydrocarbons.
Similar to:DB-HT Sim Dis, DB-2887, BPX1, Rtx-2887,HP-1, Petrocol™ 2887, Petrocol EX2887
See also Liners on pages 150-151.
Phase: 100% Dimethyl Polysiloxane Max Temps: 400 °C for films < 1.0 µm
370 °C for 2.65 µm filmsUSP Listing: N/A
Ordering InformationID (mm) Film Thickness (µm) Length (m) Part Number
0.10 0.10 10 260S020P
0.53 0.10 10 260S025P
0.53 0.90 10 260S250P
0.53 2.65 6 260S347S
0.53 2.65 10 260S348P
Analysis of standard mix for ASTM D2887
TR-SimDist, 10 m x 0.53 mm x 2.65 µmPart Number: 260S348PInitial Temp: 40 °CRate: 15 °C/minFinal Temp: 350 °C, 10 minDetector Temp: 400 °CCarrier Gas: Helium, 20 mL/minInitial Temp: 80 °CRate: 15 °C/minFinal Temp: 350 °C, 10 min
TRACE GC Columns
To order or for customer support, please see back cover.58
Phase: Cyanopropylphenyl PolysiloxaneMax Temps: 280 °C/300 °CUSP Listing: G43
TRACE TR-V1 GC ColumnsMid-polarity, thick film GC column
• Thick films for the analysis of volatile analytes• Low bleed suitable for MS detection• Ideal for EPA Methods 624 and 608
The Thermo Scientific TRACE™ TR-V1 columnis a cyanopropylphenyl-based phase ideal forthe analysis of volatile compounds. Theselectivity and film thickness of TR-V1 ensuresgood retention and separation of volatilecompounds at low oven temperatures. Thecolumn has a high maximum operating temperature of 300 °C which provides versa-tility for samples containing less volatile
compounds. The column can also be heatedquickly to remove contaminants thus reducingcycle times and increasing sample throughput.
ApplicationsThe TRACE TR-V1 is ideal for the analysis ofvolatile compounds, including use with EPAMethods 624 and 502, with good retentionand separation at low oven temperatures. The
TR-V1 provides excellent resolution from boththe front and back ends of chromatograms.
Similar to:DB-624, BPX Volatiles, Rtx Volatiles, VOCOL 56,OV-624, AT-624, HP-VOC, CP-Select™ 624CB,007-624, ZM-624
See also Syringes on page 163.
Ordering InformationID (mm) Film Thickness (µm) Length (m) Part Number
0.18 1.00 20 260V495P
0.18 1.00 40 260V496P
0.25 1.40 30 260V332P
0.25 1.40 60 260V333P
0.32 1.80 30 260V339P
0.32 1.80 60 260V341P
0.53 3.00 30 260V396P
0.53 3.00 60 260V408P
0.53 3.00 75 260V490P
Analysis of US EPA 502.2 mix
TR-V1, 30 m x 0.25 mm x 1.4 µmPart Number: 260V332PUSEPA 502.2 mix: 200 ppm in Methanol Injection Mode: SplitSplit Ratio: 50:1Injection Volume: 1 µLInitial Temp: 40 °C, 1 min Injection Temp: 250 °CRate 1: 6 °C/min to 210 °C Rate 2: 15°/min to 240 °C Liner Type: 4 mm ID Single Taper LinerFinal Temp: 240 °C, 5 minCarrier Gas: Helium, 22.8 psi Carrier Gas Flow: 1.3 mL/min Average Linear Velocity: 35 cm/sec at 40 °CDetector Type: Mass SpectrometerFull scan: 45 - 450
Components1. Dichlorodifluoromethane 2. Chloromethane 3. Vinyl chloride 4. Bromomethane 5. Chloroethane 6. Trichlorofluoromethane 7. 1,1-Dichloroethene 8. Dichloromethane 9. trans-1,2-Dichloroethene 10. 1,1-Dichloroethane 11. 2,2-Dichloropropane 12. cis-1,2-Dichloroethene 13. Bromochloromethane14. Chloroform 15. 1,1,1-Trichloroethane 16. 1,1-Dichloropropene 17. Carbon tetrachloride 18. Benzene 19. 1,2-Dichloroethane 20. Trichloroethene
21. 1,2-Dichloropropane 22. Dibromomethane 23. Bromodichloromethane 24. cis-1,3-Dichloropropene 25. Toluene 26. trans-1,3-Dichloropropene 27. 1,1,2-Trichloroethane 28. Tetrachloroethene 29. 1,3-Dichloropropane 30. Dibromochloromethane 31. 1,2-Dibromoethane32. Chlorobenzene 33. Ethylbenzene 34. 1,1,1,2-Tetrachloroethane 35. m-Xylene 36. p-Xylene 37. o-Xylene 38. Styrene 39. Bromoform40. Isopropylbenzene 41. Bromobenzene
42. 1,1,2,2-Tetrachloroethane43. 1,2,3-Trichloropropane44. n-Propyl benzene45. 2-Chlorotoluene46. 1,3,5-Trimethylbenzene
47. 4-Chlorotoluene48. tert-Butylbenzene49. 1,2,4-Trimethylbenzene50. sec-Butylbenzene51. 1,3-Dichlorobenzene
52. p-Isopropyltoluene53. 1,2-Dichlorobenzene54. n-Butylbenzene55. 1,4-Dichlorobenzene56. 1,2-Dibromo-3-chloropropan
57. 1,2,4-Trichlorobenzene58. Hexachlorobutadiene59. Naphthalene60. 1,2,3-Trichlorobenzene
GC and GC/MS
www.thermo.com /columns 59
TRACE TR-FAME GC ColumnsHigh polarity phase optimized for FAME analysis
• Silphenylene based phase• Low bleed for mass spectrometry use• Separation of fatty acid methyl esters• Separation of cis/trans isomers
The Thermo Scientific TRACE™ TR-FAME col-umn is a cyanopropylphenyl-based phasespecifically designed for the separation ofFatty Acid Methyl Esters (FAMEs). Theunique selectivity of the TR-FAME columnprovides excellent separation of cis/transisomers and FAMEs with various degrees of unsaturation. The maximum allowableoperating temperature of 260 °C also provides versatility for the analysis of high molecular weight FAMEs.
ApplicationsThe TRACE TR-FAME is ideal for the separation of cis/trans isomers and fattyacid methyl esters (FAMEs) with variousdegrees of unsaturation. It is also suitablefor any application that requires a high polarity column for use with MS detection.
Similar to:DB-23, BPX70, Rtx-2330, SP-2330, CP-Sil 88,SP-2380, HP-23, VF-23ms, 007-23, AT-Silar,PE-23
See also Gas Filters on pages 150-151.
Phase: 70% CyanopropylPolysilphenylene-siloxane
Max Temps: 250 °C/260 °CUSP Listing: N/A
Ordering InformationID (mm) Film Thickness (µm) Length (m) Part Number
0.10 0.20 10 260M096P
0.22 0.25 25 260M135P
0.22 0.25 30 260M141P
0.22 0.25 50 260M147P
0.22 0.25 60 260M153P
0.25 0.25 30 260M142P
0.25 0.25 60 260M154P
0.25 0.25 120 260M166L
0.32 0.25 25 260M137P
0.32 0.25 30 260M143P
0.32 0.25 50 260M149P
0.32 0.25 60 260M155P
Analysis of 13 sugar component alditol acetate mixture on TR-FAME
TR-FAME, 30 m x 0.25 mm x 0.25 µmPart Number: 260M142PInitial Temp: 190 °C, 1 minProgram Rate: 3 °C/minFinal Temp: 260 °C, 10 minCarrier Gas: He, 50 kPaDetector: MS (Electron Impact
Ionization, Ionization Potential 70eV) Scan 100 m/z to 350 m/z in 0.3s
Injection Mode: Split 50:1
Components1. Erythritol2. 2-Deoxy-ribitol3. Rhamnitol4. Fucitol5. Ribitol6. Arabinitol7. Xylitol8. 2-Deoxy-glucitol9. Allitol10. Mannitol11. Galacitol12. Glucitol13. Myo-inositol
TRACE GC Columns
To order or for customer support, please see back cover.60
Application Specific TRACE GC ColumnsA range of dedicated application TRACE GC columns engineered and validated for specific industry methods and applications
• Engineered with the same low bleed and temperature stable performance of the TRACE™ range of columns
• Dedicated columns for a range of EPA methodologies (524, 525, 527, 8270, 8095)• Columns for BioDiesel, Dioxins and Drugs of Abuse methods and applications• The same columns found in the Thermo Scientific Productivity Solutions for the DSQ II GC/MS
In addition to the standard range of ThermoScientific TRACE GC columns, we also providea range of application specific columns, tailored for specific industry methods andapplications. These columns have beendesigned, manufactured and tested to providespecific solutions for chromatographers analyzing samples using these industry testmethods and are also incorporated into productivity solution kits in some cases.
EPA Method Specific ColumnsWe offer specific application columns for the determination of Volatile OrganicCompounds (VOCs) and Semi-VolatileOrganic Compounds using the EPA methods524, 525 and 8270. The Thermo ScientificTRACE columns TR-524 and TR-525 are usedin the US EPA Drinking Water Test Methods524 and 525 respectively, and the TR-8270for use in the US EPA Solid Waste TestMethod 8270.
Additionally for analysis using the EPA8095 method, the TRACE TR-8095 has beentested with the EPA 8095 explosives mixtureand combines a high maximum temperatureand low surface activity to make it ideal forthis method. Also, for the determination ofselected pesticides and flame retardants usingthe US EPA Drinking Water Test Method 527,the TRACE TR-527 provides robust, low bleedperformance designed to meet the EPAmethod requirements for these analytes.
Columns for BioDiesel AnalysisBioDiesel is an important component in thedevelopment of carbon neutral fuels andspecific GC columns within the TRACE rangemeet the methodology demands for thisapplication area. A combination of columnsare offered, to cover the analytical requirements of the BioDiesel methods todetermine the methanol (TR-BioDiesel (M)),FAMEs (TR-BioDiesel (F)) and glycerides (TR-BioDiesel (G)).
GC and GC/MS
www.thermo.com /columns 61
Components1. C12:02. C14:03. C16:04. C16:15. C17:0 (Internal standard)6. C18:07. C18:1n9cis8. C18:2n6cis9. C18:3n310. C20:011. C24:0
Biodiesel F, 30 m x 0.25 mm x 0.25 µm Part Number: 26AX142PTemp: 200 °C isothermalInlet Temp: 250 °CDetector Type: FIDDetector Temp: 250 °C Carrier Gas: HeliumFlow Rate: 1.6 mL/minuteSplit Ratio: 50:1Injection Volume:1.0 µL
1
2
3
4
5
6
78
910
11
260X110
Total ion chromatogram for a soil extract spiked to contain 200 pg/µL of certain components (HMX at 2,000 pg/µL).
Components1. Nitrobenzene2. 2-Nitrotoluene3. 3-Nitrotoluene4. 4-Nitrotoluene5. Nitroglycerin6. 2,6-Dinitrotoluene-D3 (ISTD)7. 2,6-Dinitrotoluene8. 1,3-Dinitrobenzene9. 2,4-Dinitrotoluene
TR-8095, 12 m x 0.32 mm x 0.25 µm Part Number: 260P123PSample: 200 pg/µL (HMX: 2,000 pg/µL)Initial Temperature: 80 °C, 5 min.Rate #1: 20.0 °C/min to 225 °CFinal Temperature #1: 225 °CDetector Type: Mass SpecReagent Gas Type: Methane Reagent Gas Flow, all segments: 2.5 mL/minCarrier Gas: HeliumCarrier Gas Flow: 5.0 mL/minLeft Carrier Method Mode: Constant Flow Injection mode: BasicSample volume (µL): 1.0
10. 2,4,6-Trinitrotoluene11. 1,3,5-Trinitrobenzene12. PETN13. 4-Amino-2,6-dinitrotoluene14. RDX15. 3,5-Dinitroaniline16. 2-Amino-4,6-dinitrotoluene17. Tetryl18. HMX
TRACE GC Columns
To order or for customer support, please see back cover.62
Ordering InformationPhase ID (mm) Film Thickness (µm) Length (m) Use Part Number
TR-524 0.18 1.0 20 Volatile Organic Compounds 26RV495P
TR-525 0.25 0.25 30 Semi-Volatile Organic Compounds 26RX142P
TR-527 0.25 0.25 30 Selected Pesticides and Flame Retardants 26RF142P
TR-8095 0.32 0.25 12 Explosives 260P123P
TR-8270 0.25 0.5 30 Semi-Volatile Organic Compounds 26RF223P
TR-8270 0.25 1.0 30 Semi-Volatile Organic Compounds 26RF296P
TR-DoA5 0.25 0.25 15 Amphetamines 26AF130P
TR-DoA35 0.20 0.33 15 Carboxy-THC 26AC497P
TR-BioDiesel (M) 0.32 3.0 30 BioDiesel (Methanol) 26AA395P
TR-BioDiesel (F) 0.25 0.25 30 BioDiesel (FAMEs) 26AX142P
TR-BioDiesel (G) 0.32 0.1 10 BioDiesel (Glycerides) 26AF024P
TR-Dioxin 5MS 0.25 0.1 60 Dioxins 26AF059P
TR-Dioxin 5MS 0.25 0.25 60 Dioxins 26AF154P
TR-Dioxin 5MS 0.25 0.1 30 Dioxins 26AF047P
TR-PCB 8MS 0.25 0.25 50 PCBs 26AJ148P
Application Specific TRACE GC Columns
Columns For Drugs of AbuseWe offer two columns specifically designed forthe analysis of common drugs of abuse. TheThermo Scientific TR-DoA5 column is widelyused for the analysis and determination of a range of toxicological target compoundsincluding amphetamines, codeine and mor-phine. The higher polarity of the TR-DoA35column makes it ideal for the determinationof Carboxy-THC and is the recommended column for use in drug testing labs for theconfirmation of THC.
Columns for Dioxin and PCB AnalysisTo meet the requirements for High ResolutionGC/MS methods for the analysis of polychlo-rinated di-benzo-ρ-dioxins (PCDDs), polychlorodi-benzofurans (PCDFs) and polychlorinatedbi-phenyls (PCBs), we have designed a rangeof dedicated TRACE columns. These TRACEcolumns ensure optimum performance for HRGC/MS with exceptional stability and verylow bleed. The TRACE columns developed fordioxin and PCB are low polarity and providewide coverage of the 17 congeners with highest toxicological significance (2,3,7,8 – chlorine substituted congeners).
TR-DoA35, 15 m x 0.2 mm x 0.33 µm Part Number: 26AC497P
High-Throughput Confirmation andQuantitation of a THC Metabolite in Urine
Using the DSQ II GC/MS. GC/MS analysis wasperformed using the TRACE™ TR-DoA35 GC columns.
For details on the sample preparation procedureand the conditions used for the GC/MS analysis,
please request Application Note TN10161.
GC and GC/MS
www.thermo.com /columns 63
• Dramatically shorter analysis times• Increase sample throughput by a factor of 20• Lengthen column lifetimes
Ordering InformationPhase ID (mm) Film Thickness (µm) Length (m) Use Part Number
UFC-1 0.1 0.1 5 General UFMC00001010401
UFC-1 0.32 3.0 10 ASTM D-3710 UFMC00002070414
UFC-1 0.32 0.1 2.5 ASTM D-2887 UFMC00000070401
UFC-1 0.32 0.1 5 ASTM D-2887 UFMC00001070401
UFC-1 0.32 0.25 5 ISO 9377-2 UFMC00001070404
UFC-5 0.1 0.4 2.5 General UFMC00100000000
UFC-5 0.1 0.4 5 General UFMC00200000000
UFC-5 0.1 0.1 5 General UFMC00300000000
UFC-5 0.1 0.4 10 General UFMC00002010006
UFC-1701 0.1 0.1 5 General UFMC00400000000
UFC-WAX 0.1 0.2 5 General UFMC00001010503
UFC-WAX 0.1 0.1 5 FAMEs, Essential Oils UFMC00001010501
UFC-264 0.1 0.5 10 Volatiles UFMC00002010207
UFC-200 0.18 0.4 10 Alcohols/ketones UFMC00002030306
UFC-23 0.18 0.2 10 FAMEs UFMC00002030603
Columns for the Thermo Scientific UltraFast Instrument
The Thermo Scientific UltraFast TRACE™ GC isthe first integrated commercially affordablegas chromatograph with direct column heatingtechnology designed to significantly boostyour lab’s productivity.
Incorporated in a robust GC platformthis innovative technology combines a “turboheating”, linear temperature ramp of up to1,200 °C/min with short, narrow bore (0.1 mm ID) columns. By reducing the analyticalrun time by a factor of up to 20, this solutionpermits an extremely high sample/daythroughput to be achieved.
ApplicationsA large variety of column phases opens up this innovative technology to all major applications in the Chemical, Petrochemical,Environmental and Flavors and Fragrancesindustries.
The list below provides the availablecolumns, standard and non-standard, withthe dimensions and a short description oftheir use. Any existing column in our TRACEGC column range can be custom-preparedfor UFM use on request.
The UltraFast columns fit into the Thermo ScientificTRACE GC Ultra™ instrument