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  • The future of paper chromatography in the wine industry due to alternative methods

    Carien Coetzee

    SAWLA Workshop 26 May 2017

  • Paper chromatography

    Accuvin™ Vinmetrica SC-

    50

    Enzymatic Spectrometric

    Analysis

    FTIR Winescan

    HPLC

    LOQ

    Time for analyses

    Hazards

    Cost per sample

    Instrument cost

    Ease of use

    Availability

    Simpler methods Complex methods

  • Kit contains • Wide mouth jar • Capillary pipettes • Chromatography papers • Chromatography solvent

    • Tartaric acid standard • Malic acid standard • Lactic acid standard

    Paper

    chromatography

  • Paper

    chromatography

    • Small sample volume • Limited training required • Readily available • Low cost • Kit only

    • 24 hours • Hazardous chemicals • Not quantitative • Interpretation subjective

  • Malic acid

    Lactic acid

    Paper

    chromatography

  • Kit contains • Sampler • Test strip • Colour chart

    Accuvin™

  • Accuvin™

    • Small sample volume • Limited training required • NOT Readily available ? • Low-medium cost

    • Quantitative • 4 minutes • No Hazardous chemicals • Interpretation subjective • Kit only

  • • Vinmetrica SC-55 Manometer • Malic Reagent set • Biopressure Agent Reaction Vials • 1 ‘check’ vial • 10 ml serological pipette • 15 ml conical tube • Plastic bulb pipette • 100 mL polypropylene beaker • Plastic Scoop

    • Distilled water • Microwave oven

    Vinmetrica

    SC-50

  • Vinmetrica

    SC-50

    • Small sample volume • Limited training required (more than previous) • Not Readily available ? • Low-medium cost

    • Quantitative • 30-60 minutes • No Hazardous chemicals • Need to acquire instrumentation + kit

  • • Spectrophotometer • Cuvettes • Micropipettes • Enzyme test kit (includes buffer solution, NAD,

    enzymes, standards)

    Enzymatic

    Spectrometric

    Analysis

  • Enzymatic

    Spectrometric

    Analysis

    • Small sample volume • Laboratory training required • Readily available • Low-medium cost per sample

    • Quantitative • 30 minutes • No Hazardous chemicals • Need to acquire instrumentation + kit

    Measure absorbance using a spectrophometer

  • Enzymatic

    Spectrometric

    Analysis

    • Auto analyzers are convenient, but costly to purchase ✓ Eliminates analyst variation ✓ Less labour intensive ✓ Small volume needed ✓ Capacity for increased sample numbers ✓ Decrease in cost per sample

    • Skilled personnel • Periodic maintenance

  • • Foss Winescan / OenoFoss

    FTIR

    Winescan

  • FTIR

    Winescan

    • Winescan/OenoFoss is convenient, but costly to purchase ✓ Eliminates analyst variation ✓ Less labour intensive – very easy to use ✓ Small volume needed ✓ Capacity for increased sample numbers ✓ Decrease in cost per sample ✓ Automization available

    • Skilled personnel • Periodic maintenance • Constant checks due to matrix effects

    • Small sample volume • Laboratory training required • Low-medium cost per sample

    • Quantitative • 1 minute • No chemicals • Need to acquire instrumentation

  • • HPLC, column and assorted equipment • Sample vials and caps • Filtration membranes and syringes • Acetonitrile, phosphoric acid, and distilled water • Filtration unit for reagents

    HPLC

  • HPLC

    • Skilled personnel • Periodic maintenance

    • Small sample volume • Laboratory training required • High cost per sample

    • Quantitative • 40 minutes • Potentially hazardous chemicals • Need to acquire expensive

    instrumentation

  • Paper chromatography

    Accuvin™ Vinmetrica SC-

    50

    Enzymatic Spectrometric

    Analysis

    FTIR Winescan/OenoFoss

    HPLC

    Qualitative Semi-Quantitative Quantitative Quantitative Quantitative Quantitative

    LOQ n/a 0.03 g/L 0.1 g/L 0.05 g/L 0.001-0.05 g/L

    Time for analyses

    24 hours 4 min 30-60 min 30 min 1 min 40 min

    Hazards chemicals noxious,

    flamable none irritant mildly corrosive none

    chemicals flamable, corrosive, irritant

    Cost per sample

    R7 R40-R50 R40-R50 R320 (after instrument)

    R650 (after instrument)

    Instrument cost

    kit kit R2 000 R45 000 R1 200 000 R1 200 000

    Ease of use * * ** *** *** ****

    Availability **** * * *** *** ***

  • Paper

    chromatography Accuvin™

    Vinmetrica

    SC-50

    Enzymatic

    Spectrometric

    Analysis

    FTIR

    Winescan HPLC

    The future of paper chromatography in the wine industry due to alternative methods

    ✓ Easy to perform ✓ Readily available ✓ Low cost

    • Useful for wineries: in-house checks • Can then confirm with accredited laboratory the completion of MLF • Not feasible in routine laboratories

    Not Quantitative Does it need to be?

  • Questions? carien@vinlab.com

    SAWLA Workshop 26 May 2017

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