Poster abstracts / Contact Lens & Anterior Eye 36 (2013) e16–e46 e43

beneficial for the structural integrity of the cornea of contact lenswearers.

http://dx.doi.org/10.1016/j.clae.2013.08.142

80Effect of lipid-based eyedrops on siliconehydrogel contact lens wear subjectiveacceptance

Michel Guillon (PhD FCOptom) ∗, Cecile Maissa(PhD), Stephanie Wong (BSc MCOptom), AnnaLane (MOptom), Peter Simmons (PhD), JosephVehige (OD)E-mail address: mguillon@otg.co.uk (M. Guillon).

Purpose: Contact lenses cause tear film instability, increasedevaporation rate, leading to decreased ocular comfort and reducedwearing time, even with the availability of new SiHy contact lenses.The objective was to assess the effect of lipid-based eyedrops on theacceptance and subjective performance of SiHy contact lenses forsymptomatic wearers.

Method: Twenty-seven SiHy contact lens wearers, reporting dryeye symptoms (OSDI≥13) and reduced comfortable wearing timeconstituted the study population. This cross- over, sequential, ran-domised, subject-masked study tested the contact lenses after onemonth with and without the use of eyedrops. The routine involvedone enrolment visit and three series of dispensing and follow-upvisits, one for each testing condition (No eyedrop use, Optive®Plus(Test) & Blink(Control) each four times a day). Assessmentswere carried at dispensing and at the 30 day follow-up visit.

Results: The Test eyedrop was well accepted during contactlens wear. The average VA was better than 20/20, in the major-ity of cases lens fit was near optimal and the tear film stability(Mean NIBUT Control = 4.0s Test = 5.4s No drops = 4.4s) similar tothe Controls. A significantly lower OSDI score was recorded withthe Test (28.1 ± 17.9 p = 0.029) and Control (24.5 ± 18.4 p = 0.001)than without eyedrop (35.1 ± 13.8). No difference was recordedbetween the two study eyedrops (p = 0.312); Comfort was signif-icantly (p = 0.001) higher when either study eyedrop was used(Control = 70.7 Test = 70.8) than without eyedrop (55.9).

Conclusions: The study showed that Optive®Plus and Blink eye-drops tested on a population of symptomatic SiHy contact lenswearers achieved the same level of acceptance over 30 days of con-tact lens wear. The two eyedrops achieved superior comfort andlower symptomatology than when no eyedrop was used.

http://dx.doi.org/10.1016/j.clae.2013.08.143

81The effects of wearing rigid gas permeablecontact lens on tear protein concentrations

Bashirah Ishak (B.Optom (Hons), MSc) ∗, BariahMohd Ali (B. Optom (Hons), PhD), NorhaniMohidin (B. Optom, MSc, PhD)E-mail address: bashirah@medic.ukm.my(B. Ishak).

Purpose: This study aimed to examine the alteration in levels oftear proteins, namely lactoferrin, lysozyme and serum albumin (SA)after wearing hyper Dk rigid gas permeable (RGP) continuously for6 months.

Method: A total of 34 myopic subjects (neophytes), meanage of 20.71 ± 0.97 years were fitted with RGP (Dk160) contactlenses. Subjects were instructed to wear the contact lenses con-tinuously for 6 months. Another 47 myopic subjects (mean age20.57 ± 0.72) volunteered as the control group. Tears were collected

at 0 (baseline), 3 and 6 months of CL wear. Tear protein analysiswere conducted using the Bradford and electrophoresis gel sodiumdodecyl sulphate polyacrylamide (SDS-PAGE) techniques.

Results: At baseline, the TPC were 6.75 ± 1.64 mg/ml and6.10 ± 1.31 mg/ml for the study and control groups respectively.Analysis showed no significant difference between groups (Mann-Whitney, p = 0.077) at baseline and after 6 months (Friedmantest, �2 (2, n = 34) = 1.12, p = 0.572; �2 (2, n = 47) = 0.511, p = 0.775).Other protein concentrations in contact lens wearers and controlgroup at baseline were 1.10 ± 0.27 mg/ml and 1.70 ± 0.22 mg/ ml forlactoferrin, 3.51 ± 0.69 mg/ml and 3.38 ± 0.79 mg/ ml for lysozymeand 0.072 ± 0.062 mg/ml and 0.75 ± 0.053 mg/ml for SA. Statisti-cal analysis also showed no significant difference between bothgroups at baseline and after 6 months of CL wear (lactoferrin(Friedman test, �2(2, n = 34) = 2.529, p = 0.282; �2(2, n = 47) = 0.866,p = 0.648),lysozyme (Friedman test, �2(2, n = 34) = 1.824, p = 0.402;�2(2, n = 47) = 3.106, p = 0.212) and SA (Friedman Test, �2(2,n = 34) = 0.917, p = 0.632; �2(2, n = 47) = 5.144, p = 0.076)).

Conclusions: Continuous wear of hyper Dk RGP contact lens donot cause significant alterations in tear protein levels.

http://dx.doi.org/10.1016/j.clae.2013.08.144

82Confocal microscopy of the lid margin area ofcontact lens wearers

Philip Morgan (PhD MCOptom FAAO FBCLA) ∗,Ioannis Petropoulos (BOptom (Hons) MSc),Michael Read (PhD MCOptom), Rayaz Malik(MBChB FRCP PhD), Carole Maldonado-Codina(PhD MCOptom FAAO FBCLA)E-mail address: philip.morgan@manchester.ac.uk(P. Morgan).

Purpose: Contact lens (CL) discomfort remains an important butpoorly understood area for clinicians, those in the CL industry andwearers alike. Recently, there has been interest in the interactionbetween the lid margin area and the lens surface, and a possiblerelationship between this interaction and end-of-day discomfortin CL wearers.

Method: After a conventional ocular surface screening examina-tion, 10 non-CL wearers and 10 CL wearers (five using low-friction[LF, 0.018 to 0.050 Roba et al.] lenses and five using high-friction[HF, 0.166 to 0.423 Roba et al.] lenses) were examined with confo-cal microscopy (Heidelberg Retina Tomograph III Rostock CornealModule) early in the morning (before lens wear in the CL group)and then late in the afternoon (after lens wear in the CL group).

Results: Inspection of the captured confocal images revealedthe appearance of small bright spots (previously described in non-lens wearers as ‘inflammatory cells’ by others) at the proximal edgeof the lid margin in some subjects at the afternoon examination.Images were assessed in a masked manner, using a 0 to 4 gradingscale devised for this project. We found an average change frombaseline for bright spots of 0.3, 0.6 and 2.0 units for the non-wearingcontrols, the LF CL wearers and the HF CL wearers, respectively. Thisdifference was statistically significant (chi-square = 9.4, p = 0.009),with the greatest change in the HF CL group.

Conclusions: Early indications suggest changes in the lid margin(perhaps inflammatory cells) after a few hours of contact lens wearwhich may be related to the frictional properties of the lens surface,although other factors may also be important. Further explorationof these early findings is warranted.

http://dx.doi.org/10.1016/j.clae.2013.08.145