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The ChromaTographer’s ChoiCe for over 30 Years

LCGC’s mission is to enhance the productivity, effi ciency, and the overall value of separation science globally. Through unbiased peer-reviewed content, trusted troubleshooting advice, and best-practice applications solutions, we serve as a mentor to laboratory-based analytical chemists so they can enhance their profi ciency in modern chromatographic technique and instrumentation. This, in turn, provides them with a competitive advantage for the real-world analysis issues they face.

How We Achieve Our Missionat LCGC, service to our audience starts with our print products: our regular monthly issues containing peer-reviewed technical articles and installments from our renowned columnists that provide advice on core and emerging methods, troubleshooting, and the latest equipment—along with special issues, wall charts, application notebooks, and our quarterly supplement series, Current Trends in Mass Spectrometry.

We then carry our mission—and our passion for excellence—online, with our digital edition that enables readers around the globe to access the articles of our print issues; a weekly electronic newsletter (e-Separation Solutions); educational web seminars, podcasts, and The Column, a biweekly digital magazine providing troubleshooting, tips and tricks, and articles on technology in practice. Through our e-learning site “CHROMacademy,” we also provide interactive tutorials and troubleshooting.

OUR MISSION

Volume 30 Number 2 February 2012www.chromatographyonline.com

Determining the Water Content of Liquid Samples

Using Ionic Liquid–Based Capillary GC

An Interview with Harold McNair

Mass Spectrometry in Lipidomics: An Introduction

Troubleshooting an LC Autosampler Problem

Volum

e 30 Num

ber 2, 85–184 L

CG

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OR

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February 2012

Measuring Transgenic Barley Protein Expression with LC–MS

LC–MS Profi ling of the Yeast Metabolome

Characterizing Crude Oils with Direct Insertion Probe–MS

Quantitative Imaging Mass Spectrometry

S U P P L E M E N T T O

LCGC North America | LCGC Europe | SpectroscopyJuly 2012

Volum

e 30 Num

ber 5, 361–444 L

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ay 2012

Volume 30 Number 5 May 2012www.chromatographyonline.com

Pittcon 2012: New Chromatography

Columns and Accessories, Part IINew Gas Chromatography Products for 2012

Characterizing Biologics and Biosimilars

SUPPLEMENT TO

THE APPLICATION

NOTEBOOKJune 2012


THE A

PPLICATION

NO

TEBOO

K A SU

PPLEMEN

T TO LC

GC

NO

RT

H A

ME

RIC

A

JUN

E 2012

MONTHLy FeaTUReS

542 LCGC NORTH AMERICA VOLUME 30 NUMBER 7 JULY 2012 www.chromatographyonline.com

PEAKS of Interest

� Genetic and Environmental testing - 30%

� Other industries - 29%

� Agriculture and Food - 27%

� CROs - 8%

� Pharmaceutical - 6%

Bruker Opens Russian Center of ExcellenceBruker (Billerica, Massachusetts) has

opened a new applications and customer

support center of excellence in Moscow,

Russia. The facility will provide access to

a range of scientific instruments for life

science and analytical applications using

nuclear magnetic resonance (NMR), X-ray

analysis, mass spectrometry (MS), chro-

matography, molecular spectroscopy, and

atomic force microscopy.

Frank Laukien, president and CEO

of Bruker Corporation, commented,

“Bruker has come a long way since our

first projects in NMR with the Academy

of Sciences in 1970s. All of my Bruker

colleagues and I are very excited by the

new opportunities for customer collabo-

rations, training, support and applica-

tions research in Russia offered by our

outstanding new scientific and support

facility here in Moscow.”

Nikolay Yakovlev, general director of

Bruker in Russia, said, “The new Bruker

Center of Excellence offers all of our

Russian and CIS customers additional

opportunities to explore numerous

high-performance analytical instru-

ments and cutting-edge applied solu-

tions with our high-level experts here

in Moscow. This investment provides

us with the infrastructure, high-per-

formance systems and highly qualified

staff to provide comprehensive scien-

tific and engineering expertise across

many life-science and analytical tech-

nologies, for the benefit of our Russian

academic, government, clinical, and

industrial customers.”

Frisvad Receives Thought Leader AwardAgilent Technologies (Santa Clara, Cali-

fornia) has announced the recipient of

its latest Thought Leader award: Dr. Jens

Frisvad from the Center for Microbial

Biotechnology in the department of sys-

tems biology at the Technical University

of Denmark (Lyngby, Denmark).

The laboratory is working to create a

targeted metabolomics approach for

thousands of compounds produced by

Aspergillus, Penicillium, and Fusarium

Chromatography Market Profile

Continuous Flow Analysis and Discrete AnalyzersIn continuous flow analysis

(CFA), a sample is injected into a

flowing carrier solution passing

rapidly through small-bore tub-

ing. The sample is mixed with a

reagent, which reacts with the

sample to develop a color and

determine the sample concentra-

tion. The use of carefully controlled

flow conditions ensures that the color development reaction is reproducible and

the color measurement need not wait until the reaction has gone to completion.

The CFA technique of segmented flow analysis (SFA) uses turbulent flow condi-

tions that allow for complete sample dispersion. Other techniques include flow

injected analysis (FIA) and sequential injection analysis, which uses laminar flow

existing in the narrow-bore tubing to mix with the reagent to eliminate the need

for air bubble partitioning.

In contrast to CFA, discrete analyzers keep samples separate throughout the

testing process, dispensing precise amounts when required. Rotating individual

cuvettes through the instrument instead of releasing the sample in a continuous

stream cuts down on reagent waste and can produce hundreds of results per hour.

CFA and discrete analyzers are used in environmental applications for measuring

analytes such as ammonia, chloride, nitrite, and phosphate. The systems are also

used in food and beverage analysis, chemical testing, and pharmaceutical analy-

sis. Although CFA is a well-established technique for industrial applications, the

prospect of cutting testing and consumable costs, and the ease of operation have

prompted many laboratories to transfer methods from CFA to discrete analyzers.

Currently, the US and Europe account for about nearly three-fourths of the

worldwide market for CFA and discrete analyzers. Although the Latin America

and Asia Pacific markets are comparatively smaller for CFA and discrete analyzers,

these regions continue to expand rapidly and offer solid growth opportunities.

Environmental applications are estimated to account for nearly a third of the overall

demand driven by wastewater and drinking water analysis. Agriculture and food

and beverage is also a significant market with more than one-fourth of the market

share. Other industries using the technology include contract research organizations

(CROs), pharmaceutical, government testing, utilities, biotechnology, and others.

The foregoing data were extracted and adapted from SDi’s Global Assess-

ment Report, 11th Edition. For more information, contact Glenn Cudiamat, VP

of Research Services, Strategic Directions International, Inc., 6242 Westchester

Parkway, Suite 100, Los Angeles, CA 90045, (310) 641-4982, fax: (310) 641-

8851, email: [email protected].

The CFA and discrete analyzer market by industry.

fungi. Scientists will use mutable inter-

nal standards to calibrate changes in

both chromatographic retention and

MS sensitivity to compare extracts over

many years.

The award will provide the laboratory

with use of a liquid chromatography–

quadrupole time-of-flight (LC–QTOF) MS

system for discovery metabolomics, and

an LC–triple-quadrupole (QQQ) MS sys-

tem for target screening.

Frisvad commented, “Our research is

aimed at showing the impact that analy-

sis of LC–QTOF and LC–QQQ data can

have on understanding the metabolomics

of mycotoxins. We are extremely pleased

that Agilent is helping us continue this

important food-safety research.” ◾

Peaks of InterestThe latest news in the fi eld of separation science, including awards, business mergers and partnerships, research breakthroughs, and more.

Market Profi leIn-depth market analysis and data on a different chromatographic technique each month.

682 LCGC NORTH AMERICA VOLUME 29 NUMBER 8 AUGUST 2011 www.chromatographyonline.com

CALENDAR

7–11 August 2011

Microscopy & Microanalysis 2011Nashville, TN

www.microscopy.org/MandM/2011

19–22 August 2011

13th International Symposium on Electrochemical Chemistry (ISEAC)ChangChun, China

iseac.ciac.jl.cn/

21–25 August 2011

Dioxin 2011 Brussels, Belgium

www.dioxin2011.org

21–27 August 2011

5th International Conference on Quantum, Nano, and Microtechnologies (ICQNM 2011French Riviera, France

www.iaria.org/conferences2011/

ICQNM11.html

28–31 August 2011

18th International Symposium on Capillary Electroseparation Techniques (ITP 2011)Tbilisi, Georgia

www.micruxfluidic.com/events.html

28 August–1 September 2011

242nd ACS National Meeting and ExpositionDenver, CO

www.acs.org

5–9 September 2011

17th International Symposium on Separation Science (ISSS 2011)Cluj-Napoca, Romania

www.17issscluj2011.org


16th European Conference on Analytical Chemistry (EUROanalysis 2011)Belgrade, Serbia

www.euroanalysis2011.rs


62nd Annual Meeting of the International Society of ElectrochemistryNiigata, Japan

event11.ise-online.org/


8th Symposium on the Practical Applications of Mass Spectrometry in the Biotechnology IndustryRaleigh, NC

www.casss.org

14 September 2011

Bay Area Separation Science Forum: LC and LC–MSEmeryville, CA

www.casss.org


2nd European GC x GC SymposiumRegensburg, Germany

lecoetc.homeip.net/html/


1st International Symposium on Biophysical Characterization of Protein TherapeuticsRockville, MD

www.casss.org

2–6 October 2011

FACSS 2011Reno, NV

facss.org

8–12 October 2011

2nd International Laboratory Technology Conference & Exhibition (LABTECH 2011)Manama, Bahrain

www.chemistry-conferences.com/2011

9–13 October 2011

13th Symposium on the PracticalApplications for the Analysis of Proteins, Nucleotides, and Small MoleculesAmelia Island, FL

www.casss.org

14 October 2011

2011 New England Conference on Process Analytical TechnologyBedford, MA

www.necpat.com

16–19 October 2011

23rd International Ion Chromatography Symposium (IICS 2011)Providence, RI

www.casss.org

23–27 October 2011

American Association of Pharmaceutical Scientists (AAPS) Annual Meeting and ExhibitionWashington, DC

www.aaps.org/index.asp

31 October–1 November 2011

Bioassays 2011: Scientific Approaches & Regulatory StrategiesBethesda, MD

www.casss.org

14–18 November 2011

13th Conference on Instrumental AnalysisBarcelona, Spain

www.expoquimia.com/

14–16 November 2011

Eastern Analytical Symposium Somerset, NJ

www.eas.org

22 January 2012

25th CMC Strategy Forum: Winter San Francisco, CA

www.casss.org

Calendar and Short Courses a month-by-month roundup of conferences, symposia, and short course offerings from around the world of separation science.


PRODUCT RESOURCES Mass spectrometerThe Xevo G2-S QTof benchtop mass spectrometer from Waters is a quadrupole time-of-flight instrument designed with the company’s StepWave ion optics tech-nology. According to the company, the spectrometer is up to 20× more sensitive than earlier generation instruments and is UPLC compatible. Waters Corporation,Milford, MA;www.waters.com

LC systemThe Agilent 1220 Infinity LC system with diode-array detection is designed to support both HPLC and the latest UHPLC columns, including sub-2-µm and superficially porous columns. According to the company, the system is supported by the company’s Open-LAB CDS software and by third-party data systems. Agilent Technologies,Santa Clara, CA;www.agilent.com

FittingsThe Upchurch Scientific Intuitive flat-bottom fittings from IDEX Health & Science are designed for use with IVD instruments. According to the company, the product has an expanded pres-sure-holding range and “finger-tightening range” between 1.5 and 6 in.-lb. The fittings report-edly are available in PEEK and Delrin for use with 1/8- and 1/16-in. tubing.IDEX Health & Science,Lake Forest, IL; www.idexcorp.com

HPLC–UHPLC columnsPerkinElmer’s Brownlee superficially porous particle columns are designed to achieve sharper peaks and faster separation results without changing instru-ments. According to the company, the solid-core columns enable users to improve chromatography efficiency, reduce experimental run times up to 75%, and achieve UHPLC-like separations with their current HPLC system. PerkinElmer,Waltham, MA; www.perkinelmer.com

Liquid handlerGilson’s GX-241 liquid handler is designed as a compact instrument suited for applications and laborato-ries where bench space is limited. According to the company, the instrument has an injection volume range of 1 µL up to 5 mL using a minimal dead volume direct injection design. The liquid handler reportedly can accommodate sample contain-ers ranging from 2-mL vials to 100-mm test tubes.Gilson, Middleton, WI;www.gilson.com

Preparative HPLC systemThe Spot Prep II preparative HPLC system from Hitachi is designed for high-end preparative HPLC at flow rates up to 500 mL/min. According to the company, features include on-board touch-screen controls, a built-in injection valve, variable dynamic mix-ing, available quaternary gradient, and a dual-wavelength UV–vis detector that can be used to trigger fraction collection. Hitachi High Technologies America, Inc.,Schaumburg, IL; www.hitachi-hta.com

LC–MS systemThermo Fisher Scientific’s Exactive Plus benchtop LC–MS system is intended for high-throughput screening, identification, and quantification of compounds in com-plex matrices. According to the company, the system has a resolving power of up to 140,000 FWHM, a mass accuracy of better than 1 ppm in full scan and all-ion-fragmentation modes using AGC and mass calibration procedures, and a scan-ning speed of up to 12 Hz. Thermo Fisher Scientific, Waltham, MA; www.thermoscientific.com/exactiveplus

QuEChERS reagentsReagents for QuEChERS from UCT are supplied in the company’s Enviro-Clean Mylar Mini-Pouch container. The pouch is intended to simplify the addition of the reagents into a 50-mL centrifuge tube. According to the company, the pouch has a tear notch and a narrow design. UCT,Bristol, PA.www.unitedchem.com

Product Resources (products and literature) Monthly listings of the hottest products in the fi eld from leading instrumentation vendors.


COLUMN WATCH

For this month’s

installment, guest authors

Barth and Saunders discuss

the present state of size-

exclusion chromatography

(SEC) columns for the

separation of synthetic

polymers and biopolymers.

A comprehensive review

of commercially available

standard columns is

included along with

coverage of specialty

columns ranging from

those for small molecules

to ultrahigh-molecular-

weight polymers. Newer

products such as low-bleed

and high-throughput

columns are highlighted.

Column selection criteria

are suggested and future

directions in the technology

are explored.

The State of the Art and Future Trends of Size-Exclusion Chromatography Packings and Columns

Recently, we provided in-depth coverage of the basics of size-exclusion chromatography (SEC)

(1). In this installment, we expand on that coverage and discuss the present state of affairs with respect to SEC col-umns for molecular size separation of synthetic polymers and biopolymers. A comprehensive review of commercially available SEC columns from a dozen leading chromatographic companies is included ,with an emphasis on individual pore-size columns, mixed-bed packings, and wide-pore packings. Also tabulated are specialty columns for the SEC analy-sis of small molecules and oligomers, ultrahigh-molecular-weight polymers, water-soluble polymers, cationic polyelec-trolytes, polyolefins, polar and nonpolar polymers, and carbon nanotubes. “Low-bleed” columns for use with light-scat-tering detectors are considered, including columns of unusual dimensions for high-throughput and rapid analysis. For those who develop SEC methods, we also include an approach for column selection based on our combined decades of expe-rience since the inception of the method. A final section on future directions and trends of SEC column technology con-cludes this review.

Historical PerspectiveSEC is an entropically controlled sepa-ration process (2) in which molecules in solution are separated on the basis of molecular size differences, rather than by chemical composition as with enthalpic-based separations. Size separa-tion was first recognized as a new type of “partition” process in 1956 by Lathe and Ruthen (3,4) when they reported the

separation of peptides, proteins, oligosac-charides, and polysaccharides using an aqueous mobile phase and swollen starch granules. This group correctly inferred that biopolymer “retention” was caused by their differential penetration into starch granules; larger macromolecules were eluted first because they were not able to diffuse as deeply into the granules as compared to smaller macromolecules. The extent of gel penetration or pore-volume occupancy was dictated by the molecular size or molecular weight of a sample. Soon after this discovery, gel filtration got off the ground when Phar-macia (the formerly famous Swedish pharmaceutical company) introduced a series of cross-linked dextran media, called Sephadex, with controlled porosity for the separation of biopolymers (5).

After years of failed attempts by polymer chemists, John Moore of Dow Chemical Company (6) was finally able to synthesize a series of cross-linked polystyrene resin particles of known porosities for the molecular size separation of synthetic polymers. Dow Chemical Company transferred the polymerization technology to Waters Associates, which was a small instru-ment company in Framingham, Mas-sachusetts, at the time. Waters built and sold a much-needed flow-through refractometer, together with custom-made chromatographs and columns packed with cross-linked polystyrene that was synthesized in a building for-merly used as a women’s jail. As founder James Waters exclaimed (7), “GPC took off like a rocket!”It literally became an overnight success, like its Pharmacia counterpart.

Howard G. Barth and Greg D. Saunders are the guest authors of this month’s column.

Ronald E. Majors is the Column Watch Editor.


Fabrice Gritti and Georges GuiochonDepartment of Chemistry, University of Tennessee, Knoxville, Tennessee. Direct cor-respondence to: [email protected].

Facts and Legends About Columns Packed with Sub-3-μm Core–Shell Particles

Pellicular particles were invented by Csaba Horváth more than 50

years ago to prepare columns providing efficient high performance

liquid chromatography (HPLC) separations of high-molecular-weight

compounds of biological origin. However, pellicular particles met with

stiff competition from packing materials made with high-quality, fine,

fully porous particles. As a result, pellicular particles encountered little

commercial success and drifted into oblivion until, in 2007, they were

resuscitated as the modern sub-3-μm core–shell particles. This article

reports on the performance of columns packed with these new pellicular

particles, the kinetic mechanisms that underlie their exceptional

performance in the separation of low-molecular-weight compounds, and

the reasons for their current success. We demonstrate how a detailed

investigation of the band-broadening phenomena taking place in these

columns has led us to challenge beliefs that are both erroneous and

widespread throughout the HPLC community.

Pellicular particles were invented and pioneered by Horváth in the late 1960s (1–3) with the specific

purpose of preparing columns that could provide highly efficient high perfor-mance liquid chromatography (HPLC) separations of the constituents of high molecular weight compounds of biologi-cal origin. These particles are made of a solid silica core surrounded by a shell of porous silica, a design that leads to short average diffusion paths of analytes across the particles, and, therefore, a low solid-liquid mass transfer resistance (Cu) con-tribution to the van Deemter plate height equation of the column if diffusion is sluggish (8). Particles of this type were commercialized by Kirkland, who pre-pared large, 50-μm particles in the 1970s (4,5) and 5-μm particles in the 1990s (6), with the intent to pack columns that allowed for high-efficiency separations of conventional compounds. Those col-umns were not successful. Apparently, the main reason was the rapid develop-

ment between 1970 and 1990 of small, conventional, fully porous particles that provided high-efficiency columns (9). In the early 2000s, 1.7-μm fully porous par-ticles became the best performing pack-ing material, providing columns with height equivalent to a theoretical plate (HETP) terms as low as 3.3 μm (10).

In 2007, a brand of 2.7-μm superficially porous particles emerged on the market. These particles were made of 1.7-μm solid silica cores and were covered with a 0.5-μm porous shell. This design solved the problem of the low loading capacity of columns packed with the large, early pellicular particles because 75% of the volume of these particles is porous (11). Most importantly, 4.6-mm i.d. columns packed with these new particles provide efficiencies equivalent to those of columns packed with 1.7-μm classical particles, with the additional advantage of operat-ing at back pressures that are two-to-three times lower (12). Therefore, these parti-cles made it possible to achieve ultrahigh

Industry-Leading Columns Practical, nuts-and-bolts advice for your daily laboratory work, brought to you by the most respected leaders in the fi eld today.

Technical ArticlesPeer-reviewed technical research focusing on leading-edge methods, techniques, or applications of chromatography.


PEAKS of Interest

� Genetic and Environmental testing - 30%

� Other industries - 29%

� Agriculture and Food - 27%

� CROs - 8%

� Pharmaceutical - 6%

Bruker Opens Russian Center of ExcellenceBruker (Billerica, Massachusetts) has

opened a new applications and customer

support center of excellence in Moscow,

Russia. The facility will provide access to

a range of scientific instruments for life

science and analytical applications using

nuclear magnetic resonance (NMR), X-ray

analysis, mass spectrometry (MS), chro-

matography, molecular spectroscopy, and

atomic force microscopy.

Frank Laukien, president and CEO

of Bruker Corporation, commented,

“Bruker has come a long way since our

first projects in NMR with the Academy

of Sciences in 1970s. All of my Bruker

colleagues and I are very excited by the

new opportunities for customer collabo-

rations, training, support and applica-

tions research in Russia offered by our

outstanding new scientific and support

facility here in Moscow.”

Nikolay Yakovlev, general director of

Bruker in Russia, said, “The new Bruker

Center of Excellence offers all of our

Russian and CIS customers additional

opportunities to explore numerous

high-performance analytical instru-

ments and cutting-edge applied solu-

tions with our high-level experts here

in Moscow. This investment provides

us with the infrastructure, high-per-

formance systems and highly qualified

staff to provide comprehensive scien-

tific and engineering expertise across

many life-science and analytical tech-

nologies, for the benefit of our Russian

academic, government, clinical, and

industrial customers.”

Frisvad Receives Thought Leader AwardAgilent Technologies (Santa Clara, Cali-

fornia) has announced the recipient of

its latest Thought Leader award: Dr. Jens

Frisvad from the Center for Microbial

Biotechnology in the department of sys-

tems biology at the Technical University

of Denmark (Lyngby, Denmark).

The laboratory is working to create a

targeted metabolomics approach for

thousands of compounds produced by

Aspergillus, Penicillium, and Fusarium

Chromatography Market Profile

Continuous Flow Analysis and Discrete AnalyzersIn continuous flow analysis

(CFA), a sample is injected into a

flowing carrier solution passing

rapidly through small-bore tub-

ing. The sample is mixed with a

reagent, which reacts with the

sample to develop a color and

determine the sample concentra-

tion. The use of carefully controlled

flow conditions ensures that the color development reaction is reproducible and

the color measurement need not wait until the reaction has gone to completion.

The CFA technique of segmented flow analysis (SFA) uses turbulent flow condi-

tions that allow for complete sample dispersion. Other techniques include flow

injected analysis (FIA) and sequential injection analysis, which uses laminar flow

existing in the narrow-bore tubing to mix with the reagent to eliminate the need

for air bubble partitioning.

In contrast to CFA, discrete analyzers keep samples separate throughout the

testing process, dispensing precise amounts when required. Rotating individual

cuvettes through the instrument instead of releasing the sample in a continuous

stream cuts down on reagent waste and can produce hundreds of results per hour.

CFA and discrete analyzers are used in environmental applications for measuring

analytes such as ammonia, chloride, nitrite, and phosphate. The systems are also

used in food and beverage analysis, chemical testing, and pharmaceutical analy-

sis. Although CFA is a well-established technique for industrial applications, the

prospect of cutting testing and consumable costs, and the ease of operation have

prompted many laboratories to transfer methods from CFA to discrete analyzers.

Currently, the US and Europe account for about nearly three-fourths of the

worldwide market for CFA and discrete analyzers. Although the Latin America

and Asia Pacific markets are comparatively smaller for CFA and discrete analyzers,

these regions continue to expand rapidly and offer solid growth opportunities.

Environmental applications are estimated to account for nearly a third of the overall

demand driven by wastewater and drinking water analysis. Agriculture and food

and beverage is also a significant market with more than one-fourth of the market

share. Other industries using the technology include contract research organizations

(CROs), pharmaceutical, government testing, utilities, biotechnology, and others.

The foregoing data were extracted and adapted from SDi’s Global Assess-

ment Report, 11th Edition. For more information, contact Glenn Cudiamat, VP

of Research Services, Strategic Directions International, Inc., 6242 Westchester

Parkway, Suite 100, Los Angeles, CA 90045, (310) 641-4982, fax: (310) 641-

8851, email: [email protected].

The CFA and discrete analyzer market by industry.

fungi. Scientists will use mutable inter-

nal standards to calibrate changes in

both chromatographic retention and

MS sensitivity to compare extracts over

many years.

The award will provide the laboratory

with use of a liquid chromatography–

quadrupole time-of-flight (LC–QTOF) MS

system for discovery metabolomics, and

an LC–triple-quadrupole (QQQ) MS sys-

tem for target screening.

Frisvad commented, “Our research is

aimed at showing the impact that analy-

sis of LC–QTOF and LC–QQQ data can

have on understanding the metabolomics

of mycotoxins. We are extremely pleased

that Agilent is helping us continue this

important food-safety research.” ◾

RON MAJORS“Column Watch” and “Sample Prep Perspectives”Ron Majors, editor of “Column Watch” and “Sample Prep Perspectives,” has been with LCGC for over 30 years. Currently a senior scientist with Agilent Technologies, in Wilmington, Delaware, Ron is known industry-wide as one of the premier chromatography experts in the � eld. He is also a member of LCGC’s

editorial advisory board.

JOHN DOL AN“LC Troubleshooting”John Dolan has been writing “LC Troubleshooting” for LCGC for 30 years. One of the industry’s most respected professionals, John is currently a principal instructor for LC Resources, Walnut Creek,

California. He is also a member of LCGC’s editorial advisory board.

JOHN HINSHAW“GC Connections”John Hinshaw is the editor of “GC Connections” and has been a columnist with LCGC for over 27 years. John is one of the premier experts on gas chromatography active in the industry today. He is currently a senior scientist with Serveron Engineering, in Portland, Oregon, a division of BPL Global, and is a

member of LCGC’s editorial advisory board.

MICHAEL DONG“Perspectives in Modern HPLC/UHPLC”In this column, Michael W. Dong, PhD, of Genentech provides fresh scienti� c perspectives to practitioners of HPLC and UHPLC through a discussion of technical issues, innovative ideas to make chromatography less arduous, global trends and opportunities, and overviews of less-familiar separations technologies or analytical approaches.

K ATE YU“MS—The Practical Art” Kate Yu is the new editor of “MS—The Practical Art,” and will bring her expertise in the � eld of mass spectrometry and hyphenated techniques to the pages of LCGC starting in 2012. She is currently a business development manager for metabolic pro� ling with Waters Corporation, in Milford, Massachusetts.

IRA KRULL AND ANURAG RATHORE “Biotechnology Today”Ira Krull, editor of “Biotechnology Today,” is an associate professor of chemistry at Northeastern University, Boston, Massachusetts, and a member of LCGC’s editorial advisory board. Co-Editor Anurag Rathore is a consultant and associate professor with the Indian Institute of Dehli, India.

INVITED GUEST COLUMNISTS “History of Chromatography”

With each installment of this column, a different industry veteran covers an aspect of the evolution and continued development of the science of chromatography, from its birth to its eventual growth into the high-powered industry we see today.

INVITED GUEST COLUMNISTS“The Essentials”

This new column provides essential information for all types of separations – LC, GC, UHPLC, hyphenated techniques – presented in partnership with ChromAcademy.com.

With their unparalleled knowledge and technical expertise in the � eld of chromatography, our well-known and well-respected columnists share ideas and keep our readers updated on the latest industry news and information.

WORLd-ReNOWNed COLUMNISTS

advertising in a BPa-audited publication ensures your message is reaching a qualifi ed, quantifi able group of subscribers. With our audience of 54,391 BPA-qualifi ed subscribers†, you can be sure your message is being seen by subscribers in the industries and functions integral to the fi eld of chromatography.

LCGC covers the fundamental businesses and industries that make up the chromatography community†

Chromatographers rely on LCGC for information applicable to their fi eld of work^

Pharmaceuticals 41%

Medical/biological 16%

environmental 11.1%

Instrumentation/design/development 7.4%

Organic chemicals/inorganic chemicals 6.8%

agricultural/food/beverage 6.5%

energy/petroleum/bio energy 5.4%

Plastics/polymers/rubber 3.6%

Forensics/narcotics 2.2%

Readers with the following top 3 job titles rely on LCGC+

Senior, Chief, Bench Chemist/Scientist 57%

Laboratory Manager/Supervisor/R&d director/Project Head or Leader 26%

Technologist/Technician 11%

†BPa June 2012. BPa Worldwide audits LCGC’s circulation. Go to BPa’s free searchable website at www.bpaww.com.^Publisher’s Own data, May 2012. +Readex Research, March 2012

CIRCULaTION

Private industry 67.5%

Universities, colleges 12.8%

Government 8.2%

Research labs, institutes, foundations 4.6%

Hospitals, medical centers 3.1%Independent analytical labs 3%

Utilities 0.8%

Research and development 40%

Corporate management 12.4%

Quality control, assurance, validation 12%

Manufacturing, processing 8.8%

Lab management 6.9%

analysis 4.9%

Technical services 4.5%

Teaching 3.7%

Marketing, sales 3.6%

Regulatory 1.5%

Purchasing 1.2%

LCGC caters to chromatographers who perform a variety of job functions†

LCGC readers make purchasing decisions about the following techniques used^

*July 2012 Readership Study Conducted by advanstar Research Services †BPa June 2012. BPa Worldwide audits LCGC’s circulation. Go to BPa’s free searchable website at www.bpaww.com. ^Publisher’s Own data, May 2012

HPLC/UHPLC 77.8%

Gas Chromatography 57.3%

Hyphenated Techniques (LC —MS) 40.1%

Hyphenated Techniques (GC—MS) 38.3%

Ion Chromatography 25.9%

Solid Phase extraction 24.6%

Thin-Layer Chromatography 23%

Size-exclusion Chromatography 21.5%

Gel electrophoresis 15.2%

Preparative/Process Chromatography 13.2%

Chiral Chromatography 12.3%

Capillary electrophoresis/Ce-MS 12%

Low-Pressure Chromatography 11.3%

affi nity Chromatography 10.5%

Supercritical Fluid Chromatography 6.9%

Supercritical Fluid extraction 5.1%

Flash Chromatography 2%

CIRCULaTION

LCGC readers have been loyal subscribers for an average of

7.2 YEARS*

82.3% of LCGC readers

recommend, infl uence, or authorize

the purchasing of chromatography

instruments, supplies, or services*

ReadeR TReNdSFor unparalleled coverage of new chromatographic products and high-quality, unbiased technical information, our readers look to us for information and guidance on a daily basis, and as a reference throughout their careers.

When asked which publications they read regularly, 91.4% of respondents read or looked through at least 3 or 4 of the last 4 issues of LCGC*

*July 2012 Readership Study Conducted by advanstar Research Services

Readers still choose print publications most frequently when looking for information sources to keep up-to-date in their industry*

In the past 12 months, readers took the following actions as a result of seeing your advertisement in LCGC*

LCGC North america 91.4%

C&e News 36.3% american Laboratory 26.6% Lab Manager 18.3% Laboratory equipment/Chromatography Techniques 16.4% american Pharmaceutical Review 9.7%

Trade magazines/print publications 67.7% Supplier websites 51.3% Conferences 51.1% e-Newsletters 50% Web seminars/webcasts 47.8% Search engines 43.5% digital magazines 33.9% Tradeshows 30.4% Publication websites 28.2% Industry websites 21.5% Online training/videos 19.6% Social networking online 12.6% Podcasts 7.5% Blogs 5.4%

Visited a company’s website 78.8%

Recommended/discussed product/service with others 33.4% Filed advertisement for future reference 20.6% Requested information from a salesperson 15% Purchased product or service 16% Sent an email to the company 13.2% Contacted an advertiser by phone 8.6% Scanned QR codes 4.6%

Over the past fi ve years, 85.4% of readers have increased or stayed consistent in their readership of LCGC*

Reading habits decreased 14.6%

67.2% of readers have read or looked through at least 3 of the last 4 issues of LCGC*

Read 1 of 4 of the last 4 issues of LCGC 6.6%


Readers spend an average of 58 minutes reading or looking through a typical issue of LCGC*

More than 3 hours 0.8%

2 to 3 hours 8%

1 to 2 hours 27.9%

15 minutes or less 15.6%

30 minutes 26.8%

45 minutes 21%

*July 2012 Readership Study Conducted by advanstar Research Services

ReadeR TReNdS

Reading habits increased 24.1%

Reading habits haven’t changed 61.3%




eLeCTRONIC PROdUCT USaGeThrough an array of electronic products, LCGC delivers practical, nuts-and-bolts information to help our audience of scientists and lab managers become more proficient in the use of chromatographic techniques and instrumentation.

When searching the internet for chromatography products or services, the website our readers rely on the most is www.chromatographyonline.com+

www.chromatographyonline.com ................................................................................... 74.0%www.sciencedirect.com ........................................................................................................................41.9% www.acs.org ........................................................................................................................................37.6%www.journalofchromatography.com .....................................................................................................33.7% www.separationsnow.com ....................................................................................................................26.7% www.americanlaboratory.com ............................................................................................................. 20.9% www.chromatographytechniques.com ..................................................................................................19.0% www.laboratoryequipment.com ...........................................................................................................16.7% www.labmanager.com ..........................................................................................................................10.9% www.sepscience.com .............................................................................................................................9.7% www.labx.com ........................................................................................................................................ .3% www.americanpharmaceuticalreview.com ............................................................................................. 5.0% www.globalspec.com .............................................................................................................................3.1% www.labcompare.com ............................................................................................................................1.2%

Readers receive LCGC’s e-newsletters more than any others*

LCGC e-Separation Solutions ............................................................................................64.3%LCGC e-Application Note Alert .........................................................................................42.8%american Laboratorw24.5%Separation Science ................................................................................................................................ 20.1%SeparationsNOW ..................................................................................................................................19.7%Chromatography Techniques/Lab equipment .........................................................................................14.9%Lab Manager ........................................................................................................................................14.9%Select Science .......................................................................................................................................10.8%BioCompare ........................................................................................................................................... 4.8%Lab Roots .............................................................................................................................................. 4.5%Global Spec ............................................................................................................................................4.1%LabCompare .......................................................................................................................................... 2.2%

53.8% of readers consider LCGC’s e-newsletters most valuable*

LCGC e-Separation Solutions ............................................................................................ 34.7%LCGC e-Application Note Alert ..........................................................................................19.1%Lab Manager ......................................................................................................................................... 8.4%american Laboratory ..............................................................................................................................7.6%SeparationsNOW ...................................................................................................................................... 6%Separation Science ................................................................................................................................. 4.8%Select Science ........................................................................................................................................ 4.4%Chromatography Techniques/Lab equipment .......................................................................................... 2.8%BioCompare ............................................................................................................................................2.4%Global Spec .............................................................................................................................................. 2%LabCompare .......................................................................................................................................... 0.4%Lab Roots .............................................................................................................................................. 0.4%

+March 2012 e-Media Survey Conducted by advanstar Research Services*July 2012 Readership Study Conducted by advanstar Research Services

Current Trends in Mass SpectrometryGlobal Circulation: 30,000^Published in March, May, July, OctoberThe explosion of interest in mass spectrometry means chemists from a variety of backgrounds and specialties want to know more about this powerful technique. Current Trends

in Mass Spectrometry offers practical, technical, and tutorial information about mass spectrometry and its ability to solve complex analytical problems in diverse areas such as drug discovery, anti-terrorism, and environmental applications.

UHPLC/HPLCPublished in April The UHPLC/HPLC supplement brings readers an update on all that is new and innovative in the fi eld of UHPLC and HPLC technology.

2013 will mark LCGC’s 31st year of continuous publication. and although the market for separation science products has undergone dramatic changes since our debut as LC Magazine, our editorial commitment to our subscribers’ informational needs remains

constant. The high quality of LCGC’s technical articles and the practical value of its monthly columns continue to earn the magazine prestige in the chromatography community. articles contributed by the fi eld’s top names focus on issues such as sample prep, method development, data handling, nanotechnology, column technologies such as HPLC and UHPLC, and instrument troubleshooting. Comprehensive coverage of new products, new applications, and industry news secures LCGC’s position as the must-read publication for more than 54,391† subscribers involved in the separation sciences.

Annual Resources2013-2014 Annual Buyers’ Guide & Industry TrendsPublished in AugustThe most comprehensive resource for any chromatographer looking for the latest chromatography related suppliers, products or services, organized in categories specifi c to their needs, the Buyers’ Guide & Industry Trends issue continues to be a trusted

and valued resource among the most infl uential buyers in the industry.

2013 Corporate Profi le IssuePublished in DecemberThis unique advertorial publication presents you with an excellent opportunity to provide more than 50,000 of your best prospects with detailed information about your company and products.

Special SupplementsApplication Notebook Published in February, June, September, December LCGC’s application Notebook provides manufacturers with an opportunity to publish valuable information about the performance of their products under specifi c laboratory conditions. These application notes help our readers determine the usability and effi ciency

of these products for their own work. The application Notebook is a valuable resource for LCGC subscribers and manufacturers working in pharmaceutical, environmental, agricultural, food/beverage, medical/biological, drug discovery, and other application areas.

Application Notes ♦ See page 58Application Notes ♦ See page 58

Corporate Capabilities

2012 A Guide to Leading Separation Science Suppliers

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SPECIAL INDUSTRY

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2011-2012

Liquid Chromatography

Gas Chromatography

Supercritical Fluid Chromatography

Thin-Layer Chromatography

Electrophoresis

Reagents, Solvents, Standards

Data Handling, Calibration

Chromatography Services

Sample Preparation and Handling

General Scientific Equipment and Accessories

Buyers’ Guide

Measuring Transgenic Barley Protein Expression with LC–MS

LC–MS Profi ling of the Yeast Metabolome

Characterizing Crude Oils with Direct Insertion Probe–MS

Quantitative Imaging Mass Spectrometry

S U P P L E M E N T T O

LCGC North America | LCGC Europe | SpectroscopyJuly 2012

edITORIaL OVeRVIeW

†BPa June 2012. BPa Worldwide audits LCGC’s circulation. Go to BPa’s free searchable website at www.bpaww.com. ^Publisher’s Own data, May 2012

2013 HIGHLIGHTS

Ad Performance Study

LCGC has partnered with Readex Research to obtain subscriber feedback about your advertising presentation and message. advertisers who run full page, spread, half page, or fractional page ad units in the March and/or July issue(s) of LCGC will receive a bound report that provides an in-depth analysis on what our readers think about your advertisement and its effectiveness.

Results include:• Ad recall• Message awareness• Message impact• Verbatim assessments• Comparative scoring of your ad against other ads featured in the issue

6th Annual Emerging Leader in Chromatography and Lifetime Achievement in Chromatography AwardsFor the sixth consecutive year, LCGC proudly presents a unique awards program that recognizes an emerging leader and a lifetime contributor in the field of chromatography. These awards recognize the achievements and aspirations of a talented young scientific/technical professional who has made strides early in his/her career towards the advancement of chromatographic applications and techniques. at the same time, a seasoned professional will be honored for a lifetime of contributions to the advancement of chromatographic techniques and applications.

CAST TM (Custom Audience Segmentation Tool) - Right Target. Right Media. Right Now.

Meet CaSTTM the all new, highly targeted, data driven, Custom audience Segmentation Tool from advanstar’s Pharmaceutical Science Group. CaSTTM contains over 1.3 million unduplicated decision makers from global companies involved in the pharmaceutical and scientific industries served by our leading publications and conference brands.

With CASTTM we can help you…• Understand the size and scope of your potential target markets• Create new pathways to potential customers with our customized products• Direct your marketing messages to highly targeted global audiences• Drive qualified web site traffic• Focus your sales efforts on high quality leads

LCGC’s e-Learning Platform - CHROMacademy (www.CHROMacademy.com) is a membership service that offers separation scientists online access to continuing professional development training and tools, LCGC technical articles, industry news, interaction with peers and chromatography experts.

There are over 3,000 multi-media e-learning topics on HPLC, GC, MS and Sample Preparation techniques to improve practical knowledge for the absolute novice to the expert who needs a refresher in a particular topic and thousands of LCGC articles and application notes, featuring:

• Self paced modules with assessments• Laboratory tools and application notes• LCGC magazine articles• Web seminars, newsfeeds and more

Advertising Opportunities

Exclusive Channel Sponsorship – LC/HPLC, GC, Mass Spec and Sample Prep channels

CHROMacademy’s Monthly e-Newsletter (Tutorial)Features bonus content, modules, tools, and application notes. Mailed to academics, and lab managers in North america, europe and other key global centers.

Ad Sizes: 300x250 and up to four sponsored links

CHROMacademy “Essential Guide” Monthly Webcast Series Sponsorship

expert panel with audio-with-slide webcasts hosted by CHROMacademy staff and industry experts – conducted live monthly for all attendees and archived on-demand for CHROMacademy members.

Promoted via e-mail in the essential Guide Tutorial e-newsletter, posted on LCGC website/ e-newsletters; featured in Chrommunity for 1 month.

Website Stats August 2010 - 2011 August 2011 - 2012Visits 109,668 152,316Unique Visitors 46,017 73,029Page Views 253,735 433,524Pages/Visit 2.31 2.85% New Visits 39.77% 44.50% Page Views August 2010 - 2011 August 2011 - 2012Home Page 62,484 83,691HPLC Channel Page 7,516 46,171MS Channel Page 4,352 26,009GC Channel Page 3,728 24,957Sample Prep Channel Page 2,179 11,026

LCGC’S CHROMaCadeMy

LCGC sponsored web-based surveys can be used to better understand your clients’ business issues. These survey projects include a written report of findings, and can provide individual data on each survey respondent’s habits and preferences. With access to separation scientists worldwide, LCGC’s sponsored survey package offers real value in two essential ways:

1. It provides you with the opportunity to better understand your competitors influence, identify opportunities for new product development, evaluate previous marketing campaigns and help develop new strategies.

2. each global survey provides your company with a dedicated list of leads with full contact information.

Gain Market Insights

Get a better understanding of your potential customers’ needs and preferences for your products and services. you can measure market share, customer satisfaction and brand awareness for your company and your competitors.

LCGC Advantage

Our research team works with your company to produce actionable intelligence through these custom surveys. Whether your company is in need of a quick measure of industry perceptions or a detailed analysis of products and competitive positions, our custom surveys can provide the answers you need.

A Typical In-Depth Survey

Includes 10 to 15 questions

• Can include several questions with open-ended responses• 10 to 15 questions relate to products or services• 3 to 5 demographic questions• Takes between 10 to 15 minutes to complete

Statistical Analysis

The data gathered from these surveys is statistically analyzed, including cross-tabulation, and an in-depth written report of findings is prepared. The results once analyzed are correlated and a detailed report is compiled. The full report and full contact information of registrants will be provided to you along with the raw data.

Schedule

Surveys typically take two weeks to develop, including survey design, programming and invitation preparation, and then are fielded for between two to four weeks. Results are generally delivered a couple of weeks from the close date of the online survey.

For more information, please contact your sales representative.

SPONSORed SURVey PaCkaGe

Work with our editorial team to deliver a highly educational webcast with expert panelists or create your own educational webcast with your key opinion leaders.

exclusively Sponsored Webcasts: LCGC’s webcast program is a moderated online “panel” discussion conducted as a live event or pre-recorded for on-demand playback (or both) through an interface that can display slides and audio, flash video, and an array of interac-tive features like polling, chat and much more. Our webcast platform does not require any software downloads that frequently block access to these popular broadcasts.

LCGC offers a variety of webcast formats and suggested topics to provide our audiences with insights on current news, new products and applications, and important trends. Our editorial staff will work with you to customize the panel discussion/presentation to attract the most relevant listener base and to demonstrate your connection and/or expertise on the topic(s).

Beyond the benefits of associating yourself with thought leadership and high quality content, our webcasts feature a powerful lead generation tool allowing you to retrieve qualified leads from a real-time reporting platform that includes contact info, demographics and other qualifying questions from your webcast attendees and registrants. an aggressive, targeted and integrated marketing campaign is used to leverage the extensive reach of our brands to enhance your brand equity and generate a qualified audience for your sales and marketing efforts.

Content Development and Speaker Procurement

LCGC editors will assist you with content development and speaker procurement for your webcast. Webcast topics can cover all areas of separation science and all key growth segments including food analysis, bioanalysis, environmental analysis, pharmaceutical analysis, petroleum and biofuels.

Select Examples of Past Webcast Titles

• Understanding Laboratory Water Purfication• Advantages of GC-based Multi-dimensional Chromatography for Food Analysis• Using LC–MS–MS to Quantify & Identify Natural Toxins in Food & Environmental Samples• Investigating the Quality of Water Used in HPLC and LC–MS• A Milestone in Food Safety Testing

Webcast Structure

a 60-minute webcast typically consists of three panelists, moderated by an LCGC editor or other industry expert. For a three-member panel, presentations are 12–15 minutes long with audio and a rolling PowerPoint presentation. a 10–15 minute question and answer session follows the last presentation, led by the moderator, with questions taken from the audience (via e-mail) and answered live by the panelists. a webcast may also be structured with one-member or two-member panelists, depending on your interest.

Marketing Items Included in Your Webcast Package:

3 exclusive e-mails to a selection of the LCGC multimedia mailing list3 Inclusions in LCGC’s e-Newsletters2 Text listings in the LCGC multimedia mailing (one per month)1 Full page print ad1 Listing on the webcast calendar on LCGC website for 12 months1 Online ad on LCGC website

For more information, please contact your sales representative.

WeBCaST PaCkaGe

LCGC 2013 EDITORIAL CALENDAR

EDITORIAL FOCUS COLUMNS SPECIAL SUPPLEMENTBONUS

DISTRIBUTIONVALUE-ADDED

OPPORTUNITIES

ELECTRONIC SPONSORSHIP

OPPORTUNITIES

Pharmaceutical Analysis

Pre-Pittcon Issue

Space Close: December 3

LC Troubleshooting

Column Watch/Sample Prep Perspectives

GC Connections

Biotechnology Today

The Essentials

CMC Strategy Forum(CASSS)

WCBP(CASSS)

Pittcon Package (contact your sales rep for details)

Results from an e-Media Survey

4/Color Product or LiteratureRelease in Print and Online

Advertisement in Digital Edition

PITTCON Issue

Latest Chromatography Techniques

Space Close: January 7

LC Troubleshooting


MS—The Practical Art

History of Chromatography

The Essentials

Application Notebook PITTCON Pittcon Package (contactyour Sales Rep for details)

Pittcon e-Product Showcase*

Results from 2013Chromatography MarketTrends Survey

4/Color Product or LiteratureRelease in Pittcon Showcasein Print and Online

Advertisement in Digital Edition

LCGC’s Educational Webcast Series: HPLC/UHPLC

UHPLC/HPLC

EnvironmentalFood Safety

Space Close: February 4

LC Troubleshooting


GC Connections

The Essentials

Current Trends in Mass Spectrometry - 30,000 Global Distribution

FREE Ad Performance Study conducted by Readex Research

4-Color Product/Literature Release in print and online

Advertisement included inLCGC’s Digital Edition

Pittcon e-Show Daily

Pittcon Live Theatre

New Current Trends in Mass Spec global e-newsletter

Pittcon Columns & Accessories Review Part 1

Pittcon Review of HPLC Systems & Accessories

Space Close: March 4

LC Troubleshooting




Perspectives in Modern HPLC/UHPLC

The Essentials

HPLC/UHPLC Interphex

MSB (CASSS)

ISCCE (CASS)

Analytica

Results from UHPLC User Survey

4/Color Product or Literature Release in Print and Online

Advertisement included in LCGC’s Digital Edition

LCGC’s Educational Webcast Series: Sample Prep

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OPPORTUNITIES


OPPORTUNITIES

Pittcon Columns and Accessories Review: Part 2

Pittcon GC Systems & Accessories

Space Close: April 1

LC Troubleshooting


GC Connections

The Essentials


Pharmaceutical Analysis – global circulation

ASMS

Forum LABO & Biotech

ASMS e-Product Showcase*

Results from GC User Survey



ASMS e-Show Daily

LCGC’s Educational Webcast Series: Fast GC


HPLC Conference Issue


Space Close: May 1

LC Troubleshooting




The Essentials

Application Notebook

Chirality

HPLC

PREP

HPLC e-Product Showcase*

Results from HPLC Column Survey



HPLC e-Show Daily

LCGC’s Educational Webcast Series: HPLC/UHPLC

Food & Beverage

Clinical

Sample Prep

Space Close: June 3

LC Troubleshooting


GC Connections


The Essentials

Current Trends in Mass Spectrometry – 30,000 Global Distribution

SFC

AACC

COSMOS

FREE Ad Performance Study Conducted by Readex Research

4-Color Product/Literature Release in print and online


LCGC’s Educational Webcast Series: Food & Beverage


August2013-2014 Buyer’s Guide & Industry Trends

Space Close: July 3

Distributed at all shows attended by LCGC from August 2013 through August 2014

In-Column Red Boxes in Print

Bold Listings in Print

In-Column Red Boxes in Digital Edition with Hyperlinks

Bold Listings in Digital Edition with Hyperlinks

Pharmaceutical

Environmental

Space Close: July 1

LC Troubleshooting



The Essentials

ACS Fall 4/Color Product or Literature Release in Print and Online


ACS e-Show Daily

EDITORIAL FOCUS COLUMNS SPECIAL SUPPLEMENT BONUS DISTRIBUTIONVALUE-ADDED

OPPORTUNITIES


OPPORTUNITIES

HPLC/UHPLC

LC-MS

HPLC Conference Review

Space Close: August 1

LC Troubleshooting



Biotechnology Today

The Essentials

Application Notebook

Mass Spec (CASSS)

Biotechnica



LCGC’s Educational Webcast Series: LC-MS/GC-MS

GC & GC-MS

Environmental

Food Safety

AAPS Show Issue

Space Close: September 3

LC Troubleshooting


GC Connections


The Essentials


IICS (CASSS)

Gulf Coast Conference

CE in the Biotechnology (CASSS)

AAPS

Results from Sample Prep User Survey



LCGC’s Educational Webcast Series: Environmental/Food Safety

New Current Trends in Mass Spec global e-Newsletter


EAS Show Issue

Space Close: October 1

LC Troubleshooting


GC Connections


MS-The Practical Art

The Essentials

EAS 4/Color Product or Literature Release in Print and Online


e-Show Daily

Biological Applications

Annual Editorial Index

Green Chemistry

Space Close: November 4

LC Troubleshooting


Biotechnology Today

The Essentials

Annual Corporate Pro� lesand Application Notes Issue

Distribute at all shows attended by LCGC in 2014



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*Discounted rate for issue advertisers; contact your sales representative for pricing *Discounted rate for issue advertisers; contact your sales representative for pricing

LCGC NORTH AMERICA 2013 RATES

Digital Ad Requirements

1. Digital data is required for all ad submissions. Preferred format is PDF/X-1a.Note that a standard PDF is not a preferred format, files should be a PDF/X-1a which is a PDF subset specific to printing. Publisher shall have no obligation or liability to Advertiser of any kind (including, without limitation, the obligation to offer Advertiser makegoods or any other form of compensation) if an ad is supplied to Publisher by Advertiser in any format other than our preferred formats. Non-preferred or non-acceptable formats will be charged a $150 processing fee. All files should be built to exact ad space dimensions purchased. For detailed instructions on preparing PDF/X-1a files and submitting ad files to the correct size, go to www.AdsAtAdvanstar.com or contact the production manager.

2. Publisher will not supply a faxed or soft proof for Advertiser-supplied files.

Advertiser is solely responsible for preflighting and proofing all advertisements prior to submission to Publisher. If Publisher detects an error before going to press, Publisher will make a reasonable effort to contact Advertiser to give Advertiser an opportunity to correct and resubmit Advertiser’s file before publication.

3. Accepted Method of Delivery: The preferred method of delivering ad files to Advanstar is via a web based ad uploader, www.AdsAtAdvanstar.com. Files can also be submitted on CD-R or DVD-R disc format.

4. Ad Proofs: To insure that Advertiser’s ad is reproduced correctly, a

SWOP-certified color proof that has been made from the same file that Advertiser supplies to Publisher must be provided. Publisher cannot provide Advertiser any assurances regarding the accuracy of reproduction of any ad submitted without a SWOP proof. Publisher shall have no obligation or liability to Advertiser of any kind (including, without limitation, the obligation to offer Advertiser makegoods or any other form of compensation) for any ad supplied to Publisher by Advertiser without a SWOP proof.

Four Color Rates - US Dollars 1x 3x 6x 12x 18x 24x 36x 48xFull Page 11,645 11,510 11,305 11,170 10,985 10,695 10,500 10,4002/3 Page 10,835 10,725 10,675 10,095 9,930 9,675 9,485 9,3901/2 Page Island 9,740 9,675 9,615 9,130 8,975 8,780 8,610 8,5451/2 Page 9,535 9,460 9,405 8,915 8,770 8,570 8,395 8,3301/3 Page 7,660 7,580 7,515 7,215 7,115 6,945 6,835 6,7751/4 Page 6,665 6,605 6,535 6,325 6,210 6,105 6,010 5,965

Publication Dimensions Width DepthSpread Size 14 1/2” 9 1/2”Bleed (Spread) 15 3/4” 10 3/4”

Ad Dimensions Width Depth

Trim Size 7 3/4” 10 1/2”

Full Page Bleed 8” 10 3/4”

Full Page Live Area 7” 9 3/4”

Full Page Non-Bleed 6 3/4” 9”2/3 Page 4 1/2” 9 1/2”1/2 Page Island 4 1/2” 7”1/2 Page Horizontal 6 3/4” 4 5/8”1/2 Page Vertical 3 3/8” 9 1/2”1/3 Page Square 4 1/2” 4 5/8”1/3 Page Vertical 2 1/8” 9 1/2”1/4 Page 3 3/8” 4 5/8”

Special PositionsCover 2 1,720Cover 3 1,720Cover 4 3,295Preferred Pos. 1,370

ChromatographyOnline.comChromatographyOnline.com is the premier global resource for unbiased, peer-reviewed technical information in the chromatography � eld and the separation sciences. Combining all of the resources from the regional editions of our award-winning brands (LCGC North America, LCGC Europe, and LCGC Asia Paci� c), we deliver practical, nuts-and-bolts information to help our audience of scientists and lab managers become more pro� cient in the use of chromatographic techniques and instrumentation, thereby making laboratories more productive and businesses around the world more successful.

The Column: Digital EditionThe Column is a global digital magazine delivered twice monthly to subscribers’ inboxes around the world, with readers throughout Europe, North America, Asia and the Middle East. Subscribers bene� t from a global magazine with a global perspective while retaining regular content including news, Incognito, tips and tricks, events and training, interviews, and products.

LCGC North America e-Separation Solutions e-Separation Solutions, delivered three times a month, features news, practical troubleshooting tips, technique focuses, market trends, application notes and new product information to keep you up to date with current happenings within the separation science industry. Topics include HPLC, GC, Sample Prep, LC—MS and GC—MS, and emerging techniques such as Chiral, SPE, TLC, and more.

e-Application Note AlertLCGC’s e-Application Note Alert is a monthly e-mail containing application notes categorized by technique (HPLC, GC, Hyphenated Techniques, Sample Prep, etc.) and a brief description of your application note. The reader will click on your application note description, bringing them to a lead generation form which they must � ll out to receive the entire application note. You can provide us with a PDF or a prior application note from our most recent printed Application Notebook.

e-Issue AlertLCGC’s monthly Issue Alert is deployed to readers as a preview to the monthly print and digital edition. Included in the Issue Alert are current feature articles and columns.

e-Show DailyThe e-Show Daily provides up-to-the minute show coverage, created by LCGC’s editorial staff reporting directly from the show, and e-mailed to LCGC’s online subscribers. Features include: session reviews, exhibit � oor buzz, interviews, news, links, and more!

e-Solution BroadcastYour customized HTML and text formatted e-mail blast includes live hyperlinks, your company logo, image, description, and contact information. Your message will be embedded into LCGC’s e-Solution Broadcast e-mail template and will deploy to selects you have chosen from our subscriber base under the brand name of LCGC.

LabTV Web VideosLabTV is a cost-effective platform to engage prospective buyers and create deeper brand experiences. Use our unique web video program to show and tell how your products and services solve problems for chromatography related applications. Viewers who � nd your web videos useful can forward it to a friend, enabling your message to be easily passed to other prospective buyers and in� uencers.

ELECTRONIC MEDIA

exclusively Sponsored WebcastsLCGC’s webcast program is a moderated online “panel” discussion conducted as a live event or prerecorded for on-demand playback (or both) through an interface that can display slides and audio; streaming video, and an array of interactive features like polling, chat, and much more. Our webcast platform does not require any software downloads that frequently block access to these popular broadcasts.

exclusively Sponsored Podcasts LCGC’s audio podcast is a digital recording formatted like a radio call-in show that can be listened to through a “media player” on our website without any special software, or by adding the audio feed to be automatically delivered to you online via a news service (i.e., iTunes, My Yahoo, Newsgator) for on-demand playback on laptops, PCs, and a host of mobile devices.

ELECTRONIC MEDIA

DIGITAL 1x 2x 3x 4x

The Column Belly Band/Cover Tip $8,126 $6,486 $5,674 $4,682

Full Page $5,442 $4,348 $3,803 $3,2601/2 Page $3,628 $2,899 $2,536 $2,1741/3 Page $2,902 $2,319 $2,029 $1,740Skyscraper $2,835 $1,905 $1,665 $1,429Banner $2,071 $1,657 $1,449 $1,243

CUSTOM DIGITAL PRODUCTSMicrosite Custom quotes availableNewsletter Custom quotes availableDigital magazine Custom quotes available

E-MEDIA RATESPRODUCT TyPe 2013 MOnThLy RATe DeTAILSWeBSITeHome Page/Other Site Pages 728x90 $3,150 Up to 5 rotations

300x250 $3,150 Up to 7 rotations 300x100 $1,838 Up to 5 rotations Interstitial $6,000Peel Back $6,000Page Push $5,000Video Ads Custom quotes available

Run of Site (ROS) Sponsored Link $998

Technique Zones/Sections 728x90 $998 Up to 3 rotations300x250 $998 Up to 3 rotations300x100 $788 Up to 3 rotations

neWSLeTTeRE-Separation Solutions (3x monthly) 160x600 $2,625

728x90 $2,625468x60 $2,100Text - 65 words $1,500Featured Products $1,200Application Notes $1,200 Includes website archiving

Application Note Alert (1x Month) 728x90 $2,625 Featured Note $2,100 Includes website archivingApplication Note $1,575 Includes website archiving468x60 $2,100

Issue Alert 728x90 $2,625

160x600 $2,625468x60 $2,100Text - 65 words $1,500

eSolution Broadcast $3,000 For 5,000 selects

WeBCASTLive audio w/ slides Contact your sales representative for pricingLive video from studio Contact your sales representative for pricing

PODCASTS ala carte Minimum 3 $6,3004 to 7 $9,975

WeB VIDeOS - Lab TVWeb Videos We produce video $2,625 3 monthsWeb Videos Advertiser supplies video $1,575 3 months

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