The case of the “wondering” goat07-1932

Ronald D. Tyler Jr.*Geoff Saunders*Bernard Jortner*

Dan Righter†

James Mandell‡ Gerald Campbell§

* Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, VA

† Washington Animal Disease Diagnostic Laboratory, Pullman, WA‡University of Virginia Medical Center, Charlottesville, VA

§University of Texas Medical Center, Galveston, TX

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Signalment

- “Streak”- 11-year-old Alpine-

Toggenburg cross wether

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History

• Goat has been “wondering” in pasture for past month.

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History - really

• Goat has been “wandering” in pasture for past month & star gazing.

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Gross Pathology

• No gross lesions were initially noted at necropsy.

• During sectioning of the brain a well demarcated mass was discovered in the mid-brain extending to the rostral brainstem.

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Pineal gland

http://upload.wikimedia.org/wikipedia/commons/6/6b/Illu_pituitary_pineal_glands.jpg

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Histopathology:Brain:

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Immunohistochemistry

• Cytokeratin = Positive• GFAP = Negative• Synaptophysin = Positive• S-100 = Positive• Neurofilament = Negative

– βIII-tubulin (mAb TUJ1) = Pending– S-Antigen = Pending

Synaptophysin IHC

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Immunohistochemical profiles:

  PNET (neuroblastoma) Pineocytoma

Cytokeratin Positive Positive

Synaptophysin Positive Positive

Neurofilament protein Positive Positive

GFAP Negative Negative

NSE Positive Positive

S-100 Positive Positive

class III ß-tubulin Positive Negative

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Distinguishing features  Pineocytoma Neuroblastoma (PNET)

Histologicalcharacteristics

Uniform cells that tend to form "Pineocytomatous rosettes" which are large zones of fine fibrilar processes surrounded by hyperchromatic oval nuclei. The mitotic rate is generally low.6

Uniform cells that tend to palisade and form pseudorosettes and/or Homer-Wright rosettes. Neoplastic nuclei are hyperchromatic nuclei and mitotic figures are common. There are occasional regions of dystrophic mineralization.6

SimilaritiesSimilar to medulloblastomas (reserved for neurocytic tumors of the cerebellum)1

Similar to medulloblastomas (reserved for neurocytic tumors of the cerebellum)1

Ultrastructural characteristics

(Electron Microscopy)

Cytoplasmic microtubules, dense core vesicles and possibly cilia with a 9+0 configuration.4,5

Cytoplasmic dense-core neurosecretory granules, microtubules and synapse like structures.

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RosettesHomer-Wright rosettes:Supratentorial PNET (Neuroblastoma)

Pineocytomatous rosettes:Pineocytoma

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Electron Microscopy

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Case Summary

Differential Diagnoses:Differential Diagnoses:- PineocytomaPineocytoma- PNET (Neuroblastoma)PNET (Neuroblastoma)

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References

1. Maxie MG, Youssef S: Neoplastic Diseases of the Nervous System. In: Jubb, Kennedy, and Palmer’s Pathology of Domestic Animals, ed. Maxie MG, 5th ed., v1., pp.450-451, Elsevier-Mosby, St. Louis, MO, 2007

2. Koestner A, Higgins RJ: Tumors of the Nervous System. In: Tumors in Domestic Animals, ed. Mueten DJ, 4th ed., pp.319-340, Iowa State Press, IA 2002

3. Summers BA, Cummings JF, DeLahunta A: Tumors of the central nervous system. In: Veterinary Neuropathology, ed. Summers BA, Cummings JF, DeLahunta A., pp.351-394, Mosby, St. Louis, MO, 1995

4. Dario A, Cerati M, Taborelli M, Finzi G, Pozzi M, Dorizzi A: Cytogenetic and ultrastructural study of a pineocytoma, case report. Journal of Neuro-Oncology v48: pp.131-134, 2000.

5. Fevre-Montange M, Jouvet A, Privat K, Korf HW, Champier J, Reboul A, Auera M, Mottolese C: Immunohistochemical, ultrastructural, biochemical and in vitro studies of a pineocytoma. Acta Neuropathology. v95: pp.532-539, 1998.

6. Ellison D, Love S: Neoplasms. In: Neuropathology, ed. Ellison D, Love S, 2nd ed., pp.653-684, Mosby, St. Louis, MO, 2004

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Acknowledgments & Co-Authors• Special thanks to the many consultants on this case

for lending there time and expertise.• Dr. Ronald D. Tyler Jr., Virginia Maryland Regional College of Veterinary Medicine, Anatomic Path

Resident• Dr. Geoff Saunders, Virginia Maryland Regional College of Veterinary Medicine, Anatomic Pathologist• Dr. Bernard Jortner, Virginia Maryland Regional College of Veterinary Medicine, Anatomic Pathologist• Dr. Dan Righter, Washington Animal Disease Diagnostic Laboratory, Anatomic Path Resident• Dr. James Mandell, University of Virginia Medical Center, Department of Pathology, Human

Neuropathologist• Dr. Gerald Campbell, University of Texas Medical Center, Department of Pathology, Human

Neuropathologist

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The End

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