tamucc biology & microbiology lab safety orientation science & engineering

TAMUCC Biology & Microbiology

Lab Safety Orientation Science & Engineering

Overview• Emergency Phone Number• Emergency Evacuation• Accident Reporting• Lab Attire• Hazard Communication: Chemicals &

Biohazards & Glassware• Personal Protective Equipment• First Aid

BIOLOGY & MICROBIOLOGY LAB SAFETY ORIENTATION

LAB SAFETY ORIENTATION

Campus Emergency: Call University Police Department (UPD) in case of an emergency.

• From any university phone: dial 911 or ext. 4444.

• From your cell phone: dial 361-825-4444.


Emergency Call: • If you first call 911 using your cell

phone, make sure to also notify UPD at (361) 825-4444 so they can assist in the response.

LAB SAFETY ORIENTATIONAt the beginning of the semester:• Become familiar with your building;• Know your fire exits; • Know the shortest route to the nearest

exit;• Be aware of your surroundings; • And…

LAB SAFETY ORIENTATIONFire Evacuation Procedures - in case of a fire:

• Exit the building in a calm & orderly manner.

• Use the shortest and safest passage to the exit.

• DO NOT RUN.

• Do not use the elevator.

LAB SAFETY ORIENTATIONFire Evacuation Procedures

• Alert people and pull the fire alarm on your way out.

• Meet your class at the assembly area designated by your Instructor.

• Stay at least 100 feet away from the building (or 35 long steps)


Fire Evacuation Procedures

• Do not re-enter the building until the “All Clear” has been announced.

• Regardless of whether it is a real fire alarm, false alarm, or a fire drill, you are required to follow the same procedures above.


Fire Evacuation - Helping a person with mobility impairment:

• Accompany him/her to an area of rescue assistance located at the stairwell landing.

• Call or have someone call UPD & give UPD the location.


Helping a person with mobility impairment:

• UPD will bring him/her down the stairway using an evacutrak.

• Stay with him/her until UPD arrives unless your life may be in danger.

• Do not use the elevator.


Fire Safety • Never leave a fire

unattended.• Be aware of Bunsen

burners emitting invisible flames.

• Keep flammable & combustible materials away from a heat source.

LAB SAFETY ORIENTATIONFire Safety • Report all malfunctioned

equipment to your Instructor.

• Do not use frayed, damaged electrical cords.

• Do not overload the circuit with an “electrical octopus.”

Accident Reporting – Life Threatening

For a life-threatening incident, contact UPD

• From any university phone, dial 911 or 4444.

• If an ambulance is needed, inform UPD so.



Emergency Call• If you first call 911 using your cell

phone, make sure to also notify UPD at 361-825-4444 so they will assist in the response.


Accident Reporting – Non Life-Threatening

• Report all non life-threatening incidents to your Instructor.

• Use the first aid kit in the lab.

• Go to the Health Center if needed.


Lab Attire: Upon entering the lab, you are required to wear:

• Lab coat

• Long pants

• Sturdy closed-toe closed-heel shoes

LAB SAFETY ORIENTATIONLab Attire • Shoes should cover

your feet as much as possible.

• Leather top & slip-resistant sole are also recommended.

• Without proper attire, you will be asked to leave.

LAB SAFETY ORIENTATIONLab Safety Behavior• NO FOOD, NO GUM• NO DRINK• NO COSMETICS• Never touch, taste, or

smell any chemicals

• Long hair tied back

• No horseplay (pushing, shoving, pranks, etc.)


Emergency Equipment:

Fire extinguisher or other emergency equipment may be available inside the lab.

However, using them is only on a voluntary basis.


Emergency Equipment: Use emergency equipment only if:• You are trained to use

them.• Your own safety is not

compromised by using them.

Remember: YOUR OWN SAFETY COMES FIRST.


Emergency Phone in your lab.

When the red light is flashing and/or the phone is ringing, there may be an emergency announcement.

PLEASE PICK IT UP IMMEDIATELY!

Working With Chemicals• Getting to know your chemical before

using it.

• There is a Material Data Safety Sheet for every chemical in your lab.

• Looking at a MSDS is the best way to get to know your chemical.


The Material Safety Data Sheet (MSDS) provides information regarding the chemical’s

• Physical, chemical, health properties

• Safe handling, personal protective equipment required, disposal, storage, etc.


LAB SAFETY ORIENTATIONNFPA Fire Diamond is another way to communicate chemical hazards

Potential Health Hazards of Chemicals

• Poisons: Chemicals capable of harming a person in a small amount.

• Asphyxiants: Chemicals capable of

depriving the body of oxygen.

• Sensitizers: Chemicals capable of causing an allergic reaction upon repeated exposures



• Carcinogens: Chemicals capable of producing/causing cancer in humans or animals.

• Reproductive Toxins: Chemicals capable

of affecting the male or female reproductive system or a developing fetus.



• Irritants: Chemicals capable of causing inflammation, redness or itching upon exposure.

• Corrosives: Chemicals capable of destroying or causing irreversible damage to the skin, respiratory system, eyes.


Potential Physical Hazards of Chemicals

• Cryogens: Extremely cold chemicals capable of causing fire or explosion.

• Flammables: Chemicals capable of

causing fire or explosion.

• Oxidizers: Chemicals capable of

providing oxygen to feed a fire or explosion.


Routes of Entry: Paths that chemicals can get into your body.

• Inhalation Route: Contaminants enter the body through the lungs.

• Absorption Route: Contaminants enter the body through the skin or eyes


Routes of Entry:

• Ingestion Route: Contaminants enter the body through the digestive system.

• Injection Route: Contaminants enter the body through a contaminated sharp object that breaks through skin.


Hazard Communication

• Learn about the chemicals before using them

• DO NOT use any chemicals without your Instructor’s supervision or approval.

• Do wear all personal protective equipment as required.

• When in doubt, ASK.



Personal Protective Equipment (PPE)

• You will be required to wear PPE when

working with certain chemicals.

• Follow your Instructor’s instruction.

LAB SAFETY ORIENTATIONEye Protection:

• Goggles are required while you work with chemical in the lab.

• Put them on properly and keep them on while working with chemical. Goggles on your forehead WILL NOT protect your eyes.

LAB SAFETY ORIENTATIONHand Protection:

• Gloves are required while you work with chemical or biological agents in the lab.

• Use the gloves provided to you in the lab. They are compatible with agents you work with.

Chemical in Eye:

• Flush eyes out for at least 15 minutes.

• Keep your eyes open while flushing.

• Have yourself checked out at the Health Center.


Chemical on skin:

• Flush the affected area for at least 15 minutes




Ingesting Chemical:

• Rinse your mouth out immediately.

• Seek medical care or have yourself checked out at the Health Center IMMEDIATELY, if you are on campus.

• Do not induce vomiting unless directed

to do so by the health care provider.


Inhaling Chemical:

• Get out into fresh air.



Chemical Disposal• When you are done using the chemicals,

safely dispose of them as specifically instructed by your Instructor.

Broken glassware Disposal• Dispose of broken glassware in

appropriate box.• Do not place broken glassware

in the trash.

Glassware Incident

• If you get cut from broken glassware, clean out the wound prior to bandaging it.

• Have yourself checked out at the Health Center if needed.

• If there is blood on the surface, have everyone stay away and call the Instructor IMMEDIATELY.


Handling Sharps

• Sharps such as scalpel, needles, etc. can also be hazardous in the lab.

• When using scalpel, slow down, do not use it to “saw,” do not put too much pressure on it.


Handling Sharps

• Never recap needles.

• Dispose of used sharps in “SHARP” disposal container.


Biohazard Safety

• Biohazards include: bacteria, viruses, fungi, parasites, or any body fluids.

• Pathogens: agents that cause disease.

• Bloodborne Pathogens: microorganisms that may be transmitted through direct contact with contaminated blood, bodily fluids, or tissues.


Biohazardous Material Disposal

• Dispose of all biohazardous materials as directed by your instructor.

• Do not dispose of them in the trash.


Biohazard Precaution

• Treat all human blood & bodily fluids as if they are infected.

• Use gloves and other PPE as instructed.

• Avoid touching your face with your gloves.


Biohazard Precaution

• Place all used sharp objects – both contaminated & non-contaminated (needles, scalpel blades, etc.) in a “SHARPS” container.

• Never recap needles.



Leaving the lab - when the lab session is over:

• Clean your workstation.

• Put all tools and equipment back to their proper place.

• Remove gloves and other PPE.

• Wash your hands thoroughly before leaving the lab.

QUIZ

• Click on the following link to take the online quiz.• Call E,H&S @ Ext. 5555 for any Questions.• You must pass with 100% correct.• Good luck!

• Biology Lab Safety Orientation Quiz

http://www.jotform.com/form/20115056279

Have a safe semester

tamucc biology & microbiology lab safety orientation science & engineering

Documents

safety report

material data safety

use emergency equipment

emergency announcement

chemicals physical

chemicals capable of

university phone

cell phone