The New

ArmeNiAN medicAl JourNAl

Su p p l e m e N T

Volume 9, No 3, October 2015

Y E R E V A N

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Hovhannes M. Manvelyan (Yerevan. Armenia)Hamayak S. Sisakyan (Yerevan. Armenia)

Stepan A. Avagyan (Yerevan. Armenia)

Derenik H. Dumanyan (Yerevan, Armenia)Drastamat N. Khudaverdyan (Yerevan, Armenia)Levon M. Mkrtchyan (Yerevan, Armenia)

Ara S. Babloyan (Yerevan, Armenia)Aram Chobanian (Boston, USA)Luciana Dini (Lecce, Italy)Gerasimos Filippatos (Athens, Greece)Gabriele Fragasso (Milan, Italy)Samvel G. Galstyan (Yerevan, Armenia)Spartak S. Gambarov (Yerevan, Armenia)Armen Dz. Hambardzumyan (Yerevan, Armenia)Seyran P. Kocharyan (Yerevan, Armenia)Dmitri Kordzaya (Tbilisi, Georgia)Mikhail R. Lichinizer (Moscow, Russia)Aleksandr S. Malayan (Yerevan, Armenia)Mikhail Z. Narimanyan (Yerevan, Armenia)Levon N. Nazarian (Philadelphia, USA)Linda F. Noble-Haeusslein (San Francisco, USA)Artashes M. Parsadanyan (Yerevan, Armenia)Levon A. Piruzyan (Moscow, Russia)Tamara F. Sarkisian (Yerevan, Armenia)Eduard S. Sekoyan (Yerevan, Armenia)Hakob V. Topchyan (Yerevan, Armenia)Armen A. Trchunyan (Yerevan, Armenia)Alexander Tsiskaridze (Tbilisi, Georgia)

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Mikhail Z. Narimanyan Rector of YSMU

Address for correspondence:

Phones:

Yerevan

curreNT iSSueS of medicAl ScieNce

coNfereNce ABSTRACTS

Towards The 95th Anniversary ofYerevan State Medical University

after M. Heratsi

12th-15th October 2015

THE NEW ARMENIAN MEDICAL JOURNALVol .9 (2015) . No 3 Supplement

ORGANIZING COMMITTEE AND EDITORIAL BOARD

chAirmAN: Professor Mikayel Z.NariMaNyaN, rector, ysMUheAd of ScieNTific commiTTee: Professor koNstaNtiN B. yeNkoyaN, Vice-rector for scieNce aNd research, ysMUreSpoSible SecreTAry: associate Professor seyraN P kocharyaN, the head of the diVisioN of scieNce,ysMU

EDITORIAL BOARDProfessor aVetisyaN l.r.Professor aZNaUryaN a.VProfessor BeglaryaN g.a.Professor Melik-PashayaN a.e.Professor ordUyaN s.l.Professor sargsyaN a.aProfessor sekoyaN e.s.Professor shakaryaN a.a.Professor ter-PoghosyaN h.yu.Professor VardaNyaN g.J.Professor ZilfyaN a.V.

EDITORIAL STAFFBabakhanyan E.A.Grigoryan M.H.Tevosyan N.N.Ghulyan K.A.Movsisyan M.R.Kolozyan S.I.Kocharyan N.A.desigNed By s.a. aVagyaN

coNTeNTS Abstracts of coNfereNce Current Issues of Medical Science

welcome Speech . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

bASic medicAl ScieNceS . . . . . . . . . . . . . . . . . . . . . . . . .

iNTerNAl diSeASeS . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

SurgicAl diSeASeS . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

deNTiSTry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

ophThAlmologicAl diSeASeS . . . . . . . . . . . . . . . . . . . . . .

eNT diSeASeS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

obSTeTricS, gyNecology ANd pediATricS . . . . . . . . . . . . . . .

Neurology, pSychiATry ANd medicAl pSychology . . . . . . . .

public heAlTh, hygieNe, epidemiology . . . . . . . . . . . . . . .

Studens Research . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

6

7

37

49

54

67

77

91

101

111

133

147

THE NEW ARMENIAN MEDICAL JOURNALVol .9 (2015) . No 3 Supplement

d e A r p A r T i c i p A N T S

The given appendix to the “New Armenian Medical Journal” is published in the frames of the “Current Issues of Medical Science” Conference which takes place towards the 95th anniversary of Yerevan State Medical University.

The aim of the Conference is to introduce and discuss the achievements of medical science in the previous 5 years, and the development prospects of scientific researches, to promote the science internationalization and modernization process, to connect the YSMU experi-enced and young scientists, specialists and students conducting research in the theoretical and clinical departments.

Among a number of scientific events the “Student Scientific Day”, which is being organized for the first time, makes the Conference a special one. This is a good opportunity for the students to present their own research results to listen to the invited experienced specialists which will promote the development of student research process within the University.

We are honored to publish the abstracts of YSMU faculty and other Armenian and foreign scientific-educational institutions as well. A part of the received abstracts will be intro-duced through oral and poster presentations during plenary and afternoon sessions. 3 keynote and 16 plenary presentations will be introduced by leading foreign specialists. And this Abstract Book contains 114 abstracts and 12 research works submitted by YSMU students.

It is a great pleasure to welcome the participants of the Conference. I am full of hope that the analytical results of recent scientific research works will give rise to the future activi-ties and will be opening doors for new ideas of investigations thus enhancing the involve-ment of students and young researchers in scientific-research activities of the University.We wish all of us fruitful working environment and new achievements. Let us follow the slogan of the Conference: “Research, Discover, Change”.

Head of Organizing Committee YSMU Rector, Professor M.Z. Narimanyan

Head of Scientific committee

Vice-rector for Science and Research, Professor K.B. Yenkoyan

BASIC MEDICAL SCIENCESAveTiSyAN l.g., SimoNyAN K.v.,

chAvuShyAN v.A.

AvTANdilyAN N.v., KArApeTyAN S.A., AleKSANyAN K.A.

biShAryAN m.S., KhAchATryAN p.S.

chobANyAN A.S., grigoryAN A.l., melKoNyAN A.A., mKrTchyAN o.A.

dANielyAN m.A., NAzAryAN o.A., melKoNyAN N.N., ghevoNdyAN T.A.,

KhAchATryAN v.p., SArgSyAN S.g.

dArbiNyAN l.v.

gAlSTyAN A.m., KArApeTyAN A.S., mAirApeTyAN S.Kh.

gAlSTiAN h.m., chichoyAN N.b., dumANiAN K.h.

ghAzAryAN l.f., TopchyAN h.v., SAhAKyAN A.e., mArTiroSyAN h.e., ShAKAryAN m.K.,

meliKyAN m.v,

ghAzAryAN r.K., TovmASyAN A.g.SAhAKyAN l.A.

ghuKASyAN N.h., chichoyAN N.b.

grigoryAN T.S., pApyAN A.v., bAlASANyAN m.g.

hAruTyuNyAN h.A., SAhAKyAN g.v., pogo-SyAN g.A., SoghomoNyAN A.N., ArTSruNi g.g.

hovNANyAN K.

KArApeTyAN A.g., grigoryAN v.S.

KhoSTiKyAN N.g., pApyAN A.v., mArdANyAN l.S.

THE EFFECTS OF LYCIUM BARBARUM ON INDICES OF THE FUNC-TIONAL RECOVERY OF INJURED SCIATIC NERVE IN FRUCTOSE-FED RATS

THE RELATIONSHIP OF BLOOD SERUM ARGINASE ACTIVITY AND POLY-AMINES QUANTITY DURING HUMAN BREAST CANCER

INSULINE-LIKE GROWH FACTOR-1 AND SUDDEN CARDIAC DEATH

MODEL OF NEUROGLIAL CORRELATION IN THE PROCESS OF LONG-TERM POTENTIATION (LTP)

STUDY OF MORPHO-FUNCTIONAL STATE IN THE NUCLEUS OF THE SOLI-TARY TRACT IN DELABYRINTHECTOMIZED RATS

FOURFOLD COMPARATIVE POSTUROGRAPHY

PHENOLIC FRACTION STUDY OF CALLISIA FRAGRANS (LINDL.) WOOD-SON GROWN IN HYDROPONICS AND SOIL CONDITIONS

THE ANALYSIS OF TEUCRIUM POLIUM L. ESSENTIAL OIL GROWING WILDLY IN ARMENIAN FLORA AND CULTIVATED IN HYDROPONIC AND SOIL CONDITIONS BY GC-MS METHOD

ANTIMICROBIAL MEDICINES DISPENSING PRACTICE SURVEY IN THE ARMENIAN COMMUNITY PHARMACIES

WATER-SOLUBLE COMPLEXES OF CATIONIC PORPHYRINS WITH INDIGO CARMINE AND THEIR ANTIMICROBIAL ACTIVITY

THE VOLATILE OIL ANALYSIS OF BULBOUS-ROOTED CHERVIL (CHAERO-PHYLLUM BULBOUSUM L.) GROWING WILDLY IN ARMENIA, BY CAPIL-LARY GC-MS METHOD

NEUROPROTECTIVE EFFECTS OF CITICOLINE IN CEREBRAL ISCHEMIA

BIOLOGICAL EFFECTS OF THE EXTERNAL ELECTROSTATIC FIELDS

TRANSMISSION, SCANNING ELECTRON MICROSCOPY AND X-RAY ANAL-YSISORGANIC AND NONORGANIC PARTICLES

STATISTICAL EVALUATION OF ENDOCRINE CHANGES IN LIQUIDATORS: THE EARLY AND LATE POST-ACCIDENT PERIOD

INTESTINAL TYPE OF METAPLASIA IN CASE OF DIABETUS MELLITUS TYPE 2

THE NEW ARMENIAN MEDICAL JOURNALVol .9 (2015) . No 3 Supplement

meliKSeTyAN v.S., STepANyAN A.g., bAghdASAryAN N.A., hAKobyAN A.A.,

AliKhANyAN K.b., bAghdASAryAN m.g., mirzoyAN N.r.

melKoNyAN m.m.1, huNANyAN l. S. , hAruTyuNyAN h.A., grigoryAN A.m.,

TovmASyAN N.v. , pogoSyAN g.A., zAKAryAN g.v., mANuKyAN A.l.

SemerJyAN A.b., KrASNiKov N.f., mKhiTAryAN A.g., SiSAKiAN h.S.

SheKoyAN v.A., poghoSyAN g.m., AbgAryAN K.h., SArgSyAN l.A., KhAchATryAN l.g.

SimoNyAN m.h.

TorgomyAN A., AdAmyAN S., ghAmbAryAN h., hASrATyAN h., KhudAverdyAN d., Archer c.

uliKhANyAN g.r.

vArdANyAN g.S., AghAJANov m.i.

vArdANyAN g.S., NiKoghoSyAN l.r., NAzAryAN K.b.

yeNKoyAN K., dAvTyAN T., fereSheTyAN K., hAyrApeTyAN m., mirumyAN m.,

hAruTyuNyAN A., mKhiTAryAN A., chAvuShyAN v., AghAJANov m.

zAliNyAN g., pArSAdANyAN g., NerSeSyAN A., zedgiNidze A., NAmchevAdze e., NiKurAdze T.

zilfyAN A.v., AvAgyAN S.A., mATiNyAN S.v.

THE INVESTIGATION OF ANXIOLYTIC EFFECT OF ADAMANTANE AND HYDROXYPYRIDINE DERIVATIVES, AND GABA-CONJUGATES IN RAT MODEL OF LOCAL CEREBRAL ISCHEMIA

THE EFFECTS OF α2- ADRENOBLOCKERS ON OXIDATION INTENSITY IN WHITE RATS BLOOD UNDER HIGH LEVEL NOISE CHRONIC ACTION

CARDIAC AND PULMONARY CATECHOLAMINES AND INTRACARDIAC NERVES IN ACUTE ADRENALINE-INDUCED INJURY

COMPARATIVE CHARACTERISTICS OF QUANTITATIVE CHANGHES IN CONTENTS OF IL-2 IN ORGANS OF IMMUNOGENESIS AND BLOOD SERUM IN CONDITIONS OF HYPOKINETIC STRESS OF DIFFERENT DURATION

RESEARCH AND EVALUATION OF THE SELF-TREATMENT PROCESS WITH THE NON-STEROIDAL ANTI- INFLAMMATORY DRUGS IN THE REPUBLIC OF ARMENIA

PARATHYROID HORMONE INFLUENCE ON CHONDROGENIC PROGENI-TOR CELLS DIFFERENTIATION

DYNAMICS OF THE ACCUMULATION BIOLOGICALLY ACTIVE SUB-STANCES AND COMPONENT COMPOSITION OF THE ESSENTIAL OILS OF ZIZIPHORA CLINOPODIOIDES LAM.ARMENIAN FLORA

LIPID MEDIATORS OF PAIN

MOLECULAR MODELING OF MU OPIOID RECEPTOR AS AN APPROACH FOR THE CREATION OF NEW ANALGESIC DRUGS

TRANSCRIPTIONAL FACTORS OF SOX SUPERFAMILY AND NEURO-NAL GROWTH FACTORS AS A POSSIBLE LINK BETWEEN BRAIN AND BONE MARROW

MICRONUCLEUI AND OTHER NUCLEAR ANOMALIES IN EXFOLIATED BUCCAL CELLS OF CANCER PATIENTS

THE EVALUATION OF RAT’S SKIN ELECTRICAL RESISTANCE ACCORDING TO THEIR AGE

10

"Current Issues of Medical Science"

conference12th-15th October 2015

Yerevan, Armenia

The New ArmeNiAN medicAl JourNAl, Vol.9 (2015), No3 SupplementbA S i c me d i c A l Sc i e N S

AvTANdilyAN N.v.1,

KArApeTyAN S.A.2,

AleKSANyAN K.A.1

1Department of Biochemistry, YSMU2Clinical & Biochemical Lab-oratory of National Center of Oncology after V.A. Fanarjyan

Corresponding authornv.avtandilyan@ysu.am

Received 09/24/2015; Accepted for printing 09/25/2015

THE RELATIONSHIP OF BLOOD SERUM ARGINASE ACTIVITY AND POLYAMINES QUANTITY DURING

HUMAN BREAST CANCER

iNTroducTioN. Breast cancer is the most common malignant tumor of women around the world. Therefore, great medical and scientific efforts are continually invested into understanding the disease’s pathology and finding new methods for its diagnosis and treatment [4]. Polyamine (which include putrescine, spermidine and spermine) concentrations are often increased in the blood of cancer patients [2]. It was interesting to investigate the interconnection between polyamine high production and arginase activity in blood serum during women breast cancer. Arginase (EC 3.5.3.1) is binuclear manganese metalloenzyme that catalyzes the hydrolysis of L-arginine into urea and L-ornithine (precursor for polyamines) [3].

meThodS uSed. This study was done with 28 breast cancer patients (aged from 37-72, female) who were hospitalized in the National Center of Oncology after V.A. Fanarjyan and with 7 healthy people aged from 34-69 (51±15). Among cancer group patients, 10 cases were stage I (56±9, T1N0M0, 1-2cm), 10 cases had stage II (48±11, T1-2N1M0, 2-2.7cm), 8 cases had stage III (56±12, T2-3N1-

3M0, 4.3-5.4cm). The chemoradiotherapy wasn’t applied to any of patient yet. Arginase activity in the serum was studied spectrophotometrically with a mod-ified method of Diacetyl Monoxime Urea. Polyamines quantity of serum was determined by thin layer chromatography [1].

reSulTS ANd diScuSSioN. Our studies have shown that in women breast cancer group of stage I activity of serum Arginase was increased by 28.8%, in group of stage II by 36.1% and group of stage III by 48.4% comparing to the healthy women group. Therefore, arginase enzyme seemed to be a useful biological marker in breast cancer and as an indicator of breast cancer progression. The increase of total polyamines (putrescine, spermidine, spermine) quantity com-pared with standard is 69.5%, 101% and 131%, respectively in I, II and III stages. The increase of polyamines quantity coincides with the increase of argi-nase activity, what shows the correlation between them during the disease.

Conclusion. Our recent studies have shown that during inhibition of Arginase ІІ by NG-hydroxy-L-arginine in brain and kidney of rats, the quantities of poly-amines are decreased ratably [1]. Taking into consideration our results will serve for structure-based drug design and protein-protein docking, because we suggest that arginase inhibition may have some protective effects on different types of cancers development as it inhibits ornithine and also polyamines lev-els. The synthesis of new inhibitor for Arginase, which will be harmless for organism, will allow us to change the course of cancer. Our further investiga-tions will be directed to answer to the above mentioned questions.

References1. Avtandilyan N.V., Karapetyan S.A. Relationship between liver polyamines quantity and

arginase isoenzymes activity after arginase inhibition by NG-hydroxy-L-arginine. // Na-tional Academy of Sciences of RA, Electronic Journal of Natural Sciences, 2015;1(24):3-6.

2. Kuniyasu Soda. The mechanisms by which polyamines accelerate tumor spread. // J. Ex-perimental & Clinical Cancer Research. 2011;30:95.

3. Morris Jr., S.M., Recent advances in arginine metabolism: Roles and regulation of the arginases. // Br. J. Pharmacol. 2009;157:922-930.

4. National Comprehensive Cancer Network (NCCN). – Practice Guidelines in Oncology. Version 3. 2013. Accessed at www.nccn.org on June 10, 2014.

KeywordS: breast cancer, arginase, blood serum, polyamines