Supplementary material

An transcriptional repressor ERF transcription factor confers improved drought tolerance in rice and regulates genes that are preferentially located on chromosome 11 Joungsu Joo1, Hae Jong Choi1, Youn Hab Lee1, Yeon-Ki Kim2, and Sang Ik Song1

 (1) Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728, Korea.(2) Genomics Genetics Institute, GreenGene BioTech, Inc., Yongin, Kyonggido 449–728, Korea

Address correspondence to: Sang Ik Song, Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728, Korea, Tel: 82 31 330 6276 , FAX: 82 31 321 6355 , e-mail: sisong@mju.ac.kr

Figure S1 (a)

(b)NT OsERF10a

W D D D

NT

W D D

OsERF4a

Fig. S1 Stress tolerance of transgenic plants over-expressing OsERFs during the vegetative stage. T1 transgenic plants and NT controls were grown in a greenhouse for four weeks. The plants were subjected to 7 days of drought stress followed by rehydration in a greenhouse. (a) Drought-stress tolerance test of T1 transgenic plants. (b) Drought-stress tolerance of T1 OsERF4a and OsERF10a over-expressing transgenic plants. W, well-watered; D, drought-stressed and rehydrated. Photographs were taken on the indicated days

M

101ERF4a

NT 1-1 1-2 1-3 2 3 4

M NT 1 2 3 4a 4b 5

101ERF10a

1 2 4 5b 8 109M NT

105ERF4aA B

C

Figure S2

Fig. S2 Genomic Southern blotting analyses of OsCc1:OsERF4a (a), Ai:OsERF4a (b) and

OsCc1:OsERF10a (c) plants. Ten micrograms of genomic DNA was double-digested with EcoRI and

XhoI restriction enzymes, separated on a 0.8% agarose gel, blotted onto a membrane, and hybridized

with a 32P-labeled MAR probe