sqk-lsk10 sequencing w 1d - nanopore · 2016-10-10 · lambda dna 6.3. methods and material for...

SQK-LSK108 Ligation Sequencing Kit 1D

Revision Date 03/2014

SAFETY DATA SHEET

LAMBDA DNA

According to Regulation (EU) No 453/2010

SECTION 1: IDENTIFICATION OF THE SUBSTANCE/MIXTURE AND OF THE COMPANY/UNDERTAKING

1.1. Product identifier

Product name LAMBDA DNA

Container size 88 µL

1.2. Relevant identified uses of the substance or mixture and uses advised against

Identified uses Laboratory Reagent. For research purposes only.

1.3. Details of the supplier of the safety data sheet

Oxford Nanopore Technologies Ltd.Supplier

Edmund Cartwright House,

4 Robert Robinson Avenue,

Oxford Science Park,

Oxford, OX4 4GA

UK

T: +44 (0) 845 034 7900

F: +44 (0) 845 034 7901

W: www.nanoporetech.com

E: [email protected]

1.4. Emergency telephone number

+44 (0) 845 034 7900 (Mon - Fri 09:00 - 17:00 h)

SECTION 2: HAZARDS IDENTIFICATION

2.1. Classification of the substance or mixture

Classification (EC 1272/2008)

Physical and Chemical Hazards Not classified.

Human health Not classified.

Environment Not classified.

Classification (1999/45/EEC) Not classified.

The Full Text for all R-Phrases and Hazard Statements are Displayed in Section 16.

Human health

Warning - this preparation contains a substance not yet tested completely.

Environment

The product components are not classified as environmentally hazardous. However, this does not exclude the possibility that large or

frequent spills can have a harmful or damaging effect on the environment.

2.2. Label elements

Label In Accordance With (EC) No. 1272/2008

No pictogram required.

Precautionary Statements

P280 Wear protective clothing, gloves, eye and face protection.

2.3. Other hazards

This product does not contain any PBT or vPvB substances.

SECTION 3: COMPOSITION/INFORMATION ON INGREDIENTS

3.2. Mixtures

71 /

LAMBDA DNAComposition Comments

No classified ingredients, or those having occupational exposure limits, present above the levels of disclosure.

SECTION 4: FIRST AID MEASURES

4.1. Description of first aid measures

General information

Warning - this preparation contains a substance not yet tested completely. Get medical advice/attention if you feel unwell.

Inhalation

Due to the small packaging the risk of inhalation is minimal. Move into fresh air and keep at rest. Get medical attention if any discomfort

continues.

Ingestion

Get medical attention if any discomfort continues.

Skin contact

Remove contaminated clothing. Wash skin thoroughly with soap and water for several minutes. Get medical attention promptly if

symptoms occur after washing.

Eye contact

Make sure to remove any contact lenses from the eyes before rinsing. Promptly wash eyes with plenty of water while lifting the eye lids.

Continue to rinse for at least 15 minutes. Get medical attention if any discomfort continues.

4.2. Most important symptoms and effects, both acute and delayed

Inhalation

No specific symptoms noted.

Ingestion

Not known. May cause discomfort if swallowed.

Skin contact


Eye contact

Spray in the eyes: May cause temporary eye irritation.

4.3. Indication of any immediate medical attention and special treatment needed

The severity of the symptoms described will vary dependant of the concentration and the length of exposure. Warning - this preparation

contains a substance not yet tested completely.

SECTION 5: FIREFIGHTING MEASURES

5.1. Extinguishing media

Extinguishing media

This product is not flammable. Use fire-extinguishing media appropriate for surrounding materials.

Unsuitable extinguishing media

Do not use water jet as an extinguisher, as this will spread the fire.

5.2. Special hazards arising from the substance or mixture

Hazardous combustion products

Fire creates: Carbon monoxide (CO). Carbon dioxide (CO2). Hydrocarbons.

Unusual Fire & Explosion Hazards

No unusual fire or explosion hazards noted.

Specific hazards

Due to the small packaging, the risk of inhaling gases formed in a fire is minimal.

5.3. Advice for firefighters

Special Fire Fighting Procedures

Warning - this preparation contains a substance not yet tested completely. Avoid contact with skin and eyes.

Protective equipment for fire-fighters

Self contained breathing apparatus and full protective clothing must be worn in case of fire.

SECTION 6: ACCIDENTAL RELEASE MEASURES

6.1. Personal precautions, protective equipment and emergency procedures

Wear protective clothing as described in Section 8 of this safety data sheet.

6.2. Environmental precautions

Avoid discharge to the aquatic environment.

72 /

LAMBDA DNA

6.3. Methods and material for containment and cleaning up

In case of spills, beware of slippery floors and surfaces. Wear necessary protective equipment. Absorb spillage with non-combustible,

absorbent material. Transfer to a container for disposal. For waste disposal, see section 13.

6.4. Reference to other sections

Wear protective clothing as described in Section 8 of this safety data sheet. See section 11 for more detailed information on health effects

and symptoms. For waste disposal, see section 13.

SECTION 7: HANDLING AND STORAGE

7.1. Precautions for safe handling

Read and follow manufacturer's recommendations. Wear protective clothing as described in Section 8 of this safety data sheet. Do not

handle broken packages without protective equipment. Avoid contact with skin and eyes. Do not eat, drink or smoke when using the

product. Wash hands thoroughly after handling.

7.2. Conditions for safe storage, including any incompatibilities

Protect from freezing and direct sunlight. Store at temperatures not exceeding 4°C.

7.3. Specific end use(s)

The identified uses for this product are detailed in Section 1.2.

SECTION 8: EXPOSURE CONTROLS/PERSONAL PROTECTION

8.1. Control parameters

Ingredient Comments

No exposure limits noted for ingredient(s).

8.2. Exposure controls

Process conditions

Provide eyewash station.

Engineering measures

Provide adequate ventilation.

Respiratory equipment

No specific recommendations.

Hand protection

Protective gloves must be used if there is a risk of direct contact or splash. The most suitable glove must be chosen in consultation with

the gloves supplier, who can inform about the breakthrough time of the glove material.

Eye protection

Wear approved safety goggles.

Other Protection

Wear suitable protective clothing as protection against splashing or contamination.

Hygiene measures

Wash hands after handling. When using do not eat, drink or smoke. Wash contaminated clothing before reuse.

Environmental Exposure Controls

Avoid discharge to the aquatic environment.

SECTION 9: PHYSICAL AND CHEMICAL PROPERTIES

9.1. Information on basic physical and chemical properties

Appearance Liquid

Colour Colourless.

Odour No characteristic odour.

Solubility Soluble in water.

Initial boiling point and boiling range

(°C)

100°C @ 760 mm Hg

Melting point (°C) ~0°C @ 760 mm Hg

Relative density

Not determined.

Vapour density (air=1)

Not determined.

73 /

LAMBDA DNAVapour pressure

Not determined.

Evaporation rate

Not determined.

pH-Value, Conc. Solution

Not determined.

Viscosity

Not determined.

Decomposition temperature (°C)

Not determined.

Odour Threshold, Lower

Not determined.

Odour Threshold, Upper

Not determined.

Flash point (°C)

Not determined.

Auto Ignition Temperature (°C)

Not determined.

Flammability Limit - Lower(%)

Not determined.

Flammability Limit - Upper(%)

Not determined.

Partition Coefficient

(N-Octanol/Water)

Not determined.

Explosive properties

Not considered to be explosive.

Oxidising properties

Does not meet the criteria for oxidising.

9.2. Other information

Not known.

SECTION 10: STABILITY AND REACTIVITY

10.1. Reactivity

There are no known reactivity hazards associated with this product.

10.2. Chemical stability

Stable under normal temperature conditions and recommended use. Stable under the prescribed storage conditions.

10.3. Possibility of hazardous reactions

Not known.

Hazardous Polymerisation

Will not polymerise.

10.4. Conditions to avoid

Avoid heat. Protect from freezing and direct sunlight.

10.5. Incompatible materials

Materials To Avoid

No incompatible groups noted.

10.6. Hazardous decomposition products

Not known.

SECTION 11: TOXICOLOGICAL INFORMATION

11.1. Information on toxicological effects

Toxicological information

Warning - this preparation contains a substance not yet tested completely.

74 /

LAMBDA DNA

Acute toxicity:

Acute Toxicity (Oral LD50)

No information available.

Based on available data the classification criteria are not met.

Acute Toxicity (Dermal LD50)



Acute Toxicity (Inhalation LC50)



Skin Corrosion/Irritation:


Serious eye damage/irritation:


Respiratory or skin sensitisation:

Respiratory sensitisation



Skin sensitisation



Germ cell mutagenicity:

Genotoxicity - In Vitro



Carcinogenicity:

Carcinogenicity



Reproductive Toxicity:

Reproductive Toxicity - Fertility



Reproductive Toxicity - Development



Specific target organ toxicity - single exposure:

STOT - Single exposure



Specific target organ toxicity - repeated exposure:

STOT - Repeated exposure



Aspiration hazard:

Not anticipated to present an aspiration hazard based on chemical structure.

SECTION 12: ECOLOGICAL INFORMATION

Ecotoxicity



12.1. Toxicity

75 /

LAMBDA DNAAcute Fish Toxicity

Not considered toxic to fish.

12.2. Persistence and degradability

Degradability

There are no data on the degradability of this product.

12.3. Bioaccumulative potential

Bioaccumulative potential

No data available on bioaccumulation.

Partition coefficient

Not determined.

12.4. Mobility in soil

Mobility:

The product is soluble in water.

12.5. Results of PBT and vPvB assessment


12.6. Other adverse effects

None known.

SECTION 13: DISPOSAL CONSIDERATIONS

13.1. Waste treatment methods

When handling waste, consideration should be made to the safety precautions applying to handling of the product. Residues and empty

containers should be taken care of as hazardous waste according to local and national provisions.

SECTION 14: TRANSPORT INFORMATION

General The product is not covered by international regulation on the transport of dangerous goods (IMDG, IATA,

ADR/RID).

14.1. UN number

Not applicable.

14.2. UN proper shipping name

Not applicable.

14.3. Transport hazard class(es)

Transport Labels

No transport warning sign required.

14.4. Packing group

Not applicable.

14.5. Environmental hazards

Environmentally Hazardous Substance/Marine Pollutant

No.

14.6. Special precautions for user

Not applicable.

14.7. Transport in bulk according to Annex II of MARPOL73/78 and the IBC Code

Not applicable.

SECTION 15: REGULATORY INFORMATION

76 /

LAMBDA DNA

15.1. Safety, health and environmental regulations/legislation specific for the substance or mixture

Guidance Notes

Workplace Exposure Limits EH40.

EU Legislation

Regulation (EC) No 1272/2008 of the European Parliament and of the Council of 16 December 2008 on classification, labelling and

packaging of substances and mixtures, amending and repealing Directives 67/548/EEC and 1999/45/EC, and amending Regulation (EC)

No 1907/2006 with amendments.

15.2. Chemical Safety Assessment

No chemical safety assessment has been carried out.

SECTION 16: OTHER INFORMATION

General information

Only trained personnel should use this material.

Revision Comments

This is first issue.

Revision Date 03/2014

Risk Phrases In Full

Not classified.NC

77 /

Revision date: 12/08/2015

SAFETY DATA SHEETElution Buffer

According to Regulation (EC) No 1907/2006, Annex II, as amended by Regulation (EU) No 453/2010

SECTION 1: Identification of the substance/mixture and of the company/undertaking


Product name Elution Buffer

Container size 200μl


Identified uses Laboratory reagent.

Uses advised against No specific uses advised against are identified.


Supplier Oxford Nanopore Technologies Ltd.Edmund Cartwright House,4 Robert Robinson Avenue,Oxford Science Park,Oxford,OX4 4GA,UKT: +44 (0) 845 034 7900F: +44 (0) 845 034 7901E: [email protected]


Emergency telephone +44 (0) 845 034 7900 (Mon - Fri 09:00 - 17:00 h)

SECTION 2: Hazards identification

2.1. Classification of the substance or mixtureClassificationPhysical hazards Not Classified

Health hazards Not Classified

Environmental hazards Not Classified

2.2. Label elements

Hazard statements NC Not Classified

Precautionary statements P280 Wear protective clothing, gloves, eye and face protection.

Important Notes. This product is considered to be a small package and is labelled according to the relevantprovisions of the legislation.

2.3. Other hazards

This product does not contain any substances classified as PBT or vPvB.

SECTION 3: Composition/information on ingredients

3.2. Mixtures

1/8


Elution Buffer

Composition comments No classified ingredients, or those having occupational exposure limits, present above thelevels of disclosure.,This product contains a substance that has not yet been fully tested andmay have unforeseen effects.

SECTION 4: First aid measures


General information Not considered to be a significant hazard due to the small quantities used. Get medicalattention if any discomfort continues. Show this Safety Data Sheet to the medical personnel.

Inhalation Move affected person to fresh air and keep warm and at rest in a position comfortable forbreathing. Maintain an open airway. Loosen tight clothing such as collar, tie or belt.

Ingestion Rinse mouth thoroughly with water. Maintain an open airway. Loosen tight clothing such ascollar, tie or belt. Give a few small glasses of water or milk to drink. Stop if the affected personfeels sick as vomiting may be dangerous. Do not induce vomiting unless under the direction ofmedical personnel.

Skin contact Remove affected person from source of contamination. Rinse immediately with plenty ofwater.

Eye contact Rinse immediately with plenty of water. Remove any contact lenses and open eyelids wideapart. Continue to rinse for at least 10 minutes.

Protection of first aiders First aid personnel should wear appropriate protective equipment during any rescue.


General information See Section 11 for additional information on health hazards. The severity of the symptomsdescribed will vary dependent on the concentration and the length of exposure. Notconsidered to be a significant hazard due to the small quantities used.

Inhalation Prolonged inhalation of high concentrations may damage respiratory system.

Ingestion Gastrointestinal symptoms, including upset stomach.

Skin contact Prolonged contact may cause dryness of the skin.

Eye contact May cause temporary eye irritation.


Notes for the doctor Treat symptomatically.

Specific treatments No special treatment required.

SECTION 5: Firefighting measures


Suitable extinguishing media The product is not flammable. Extinguish with alcohol-resistant foam, carbon dioxide, drypowder or water fog. Use fire-extinguishing media suitable for the surrounding fire.

Unsuitable extinguishingmedia



Specific hazards Containers can burst violently or explode when heated, due to excessive pressure build-up.Not considered to be a significant hazard due to the small quantities used.

Hazardous combustionproducts

Thermal decomposition or combustion products may include the following substances:Harmful gases or vapours.


2/8


Elution Buffer

Protective actions duringfirefighting

Avoid breathing fire gases or vapours. Evacuate area. Cool containers exposed to heat withwater spray and remove them from the fire area if it can be done without risk. Cool containersexposed to flames with water until well after the fire is out. If a leak or spill has not ignited, usewater spray to disperse vapours and protect men stopping the leak.

Special protective equipmentfor firefighters

Wear positive-pressure self-contained breathing apparatus (SCBA) and appropriate protectiveclothing. Firefighter's clothing conforming to European standard EN469 (including helmets,protective boots and gloves) will provide a basic level of protection for chemical incidents.

SECTION 6: Accidental release measures


Personal precautions No action shall be taken without appropriate training or involving any personal risk. Keepunnecessary and unprotected personnel away from the spillage. Wear protective clothing asdescribed in Section 8 of this safety data sheet. Follow precautions for safe handlingdescribed in this safety data sheet. Wash thoroughly after dealing with a spillage.


Environmental precautions Avoid discharge to the aquatic environment. Large Spillages: Inform the relevant authorities ifenvironmental pollution occurs (sewers, waterways, soil or air).


Methods for cleaning up Wear protective clothing as described in Section 8 of this safety data sheet. Reuse or recycleproducts wherever possible. If the product is soluble in water, dilute the spillage with waterand mop it up. Flush contaminated area with plenty of water. Wash thoroughly after dealingwith a spillage. Dispose of waste to licensed waste disposal site in accordance with therequirements of the local Waste Disposal Authority.


Reference to other sections For personal protection, see Section 8.

SECTION 7: Handling and storage


Usage precautions Read and follow manufacturer's recommendations. Wear protective clothing as described inSection 8 of this safety data sheet. Keep away from food, drink and animal feeding stuffs.Handle all packages and containers carefully to minimise spills. Keep container tightly sealedwhen not in use. Avoid the formation of mists.

Advice on generaloccupational hygiene

Wash promptly if skin becomes contaminated. Take off contaminated clothing. Washcontaminated clothing before reuse. Do not eat, drink or smoke when using this product.Wash at the end of each work shift and before eating, smoking and using the toilet. Changework clothing daily before leaving workplace.


Storage precautions Store in tightly-closed, original container in a dry, cool and well-ventilated place. Store attemperatures not exceeding 4°C.

Storage class Unspecified storage.


Specific end use(s) The identified uses for this product are detailed in Section 1.2.

SECTION 8: Exposure Controls/personal protection


Ingredient comments No exposure limits known for ingredient(s).

3/8


Elution Buffer


Protective equipment

Appropriate engineeringcontrols

Provide adequate ventilation. Good general ventilation should be adequate to control workerexposure to airborne contaminants.

Eye/face protection Eyewear complying with an approved standard should be worn if a risk assessment indicateseye contact is possible. Personal protective equipment for eye and face protection shouldcomply with European Standard EN166. The following protection should be worn: Chemicalsplash goggles.

Hand protection Chemical-resistant, impervious gloves complying with an approved standard should be worn ifa risk assessment indicates skin contact is possible. Considering the data specified by theglove manufacturer, check during use that the gloves are retaining their protective propertiesand change them as soon as any deterioration is detected.

Other skin and bodyprotection

Appropriate footwear and additional protective clothing complying with an approved standardshould be worn if a risk assessment indicates skin contamination is possible.

Hygiene measures Provide eyewash station and safety shower. Contaminated work clothing should not beallowed out of the workplace. Wash contaminated clothing before reuse. Clean equipmentand the work area every day. Good personal hygiene procedures should be implemented.Wash at the end of each work shift and before eating, smoking and using the toilet. Whenusing do not eat, drink or smoke.

Respiratory protection Respiratory protection complying with an approved standard should be worn if a riskassessment indicates inhalation of contaminants is possible. Provide adequate ventilation.Large Spillages: If ventilation is inadequate, suitable respiratory protection must be worn.

Environmental exposurecontrols

Not regarded as dangerous for the environment.

SECTION 9: Physical and Chemical Properties


Appearance Liquid.

Colour Colourless.

Odour Odourless.

Odour threshold Not available.

pH Not available.

Melting point ~0°C @ 760 mm Hg

Initial boiling point and range ~100°C @ 760 mm Hg

Flash point Not available.

Evaporation rate Not available.

Upper/lower flammability orexplosive limits

Not available.

Vapour pressure Not available.

Vapour density Not available.

4/8


Elution Buffer

Relative density Not available.

Solubility(ies) Soluble in water.

Partition coefficient Not available.

Auto-ignition temperature Not available.

Decomposition Temperature Not available.

Viscosity Not applicable.

Explosive properties Not considered to be explosive.

Oxidising properties Does not meet the criteria for classification as oxidising.


Other information No information required.

SECTION 10: Stability and reactivity

10.1. Reactivity

Reactivity There are no known reactivity hazards associated with this product.


Stability Stable at normal ambient temperatures and when used as recommended. Stable under theprescribed storage conditions.


Possibility of hazardousreactions

No potentially hazardous reactions known.


Conditions to avoid Avoid excessive heat for prolonged periods of time.


Materials to avoid Strong oxidising agents. Strong reducing agents.


Hazardous decompositionproducts

Does not decompose when used and stored as recommended. Thermal decomposition orcombustion products may include the following substances: Harmful gases or vapours.

SECTION 11: Toxicological information


Toxicological effects Not regarded as a health hazard under current legislation.

Acute toxicity - oralNotes (oral LD₅₀) Based on available data the classification criteria are not met.

Acute toxicity - dermalNotes (dermal LD₅₀) Based on available data the classification criteria are not met.

Acute toxicity - inhalationNotes (inhalation LC₅₀) Based on available data the classification criteria are not met.

Skin corrosion/irritationAnimal data Based on available data the classification criteria are not met.

Serious eye damage/irritation

5/8


Elution Buffer

Serious eye damage/irritation Based on available data the classification criteria are not met.

Respiratory sensitisationRespiratory sensitisation Based on available data the classification criteria are not met.

Skin sensitisationSkin sensitisation Based on available data the classification criteria are not met.

Germ cell mutagenicityGenotoxicity - in vitro Based on available data the classification criteria are not met.

CarcinogenicityCarcinogenicity Based on available data the classification criteria are not met.

IARC carcinogenicity None of the ingredients are listed or exempt.

Reproductive toxicityReproductive toxicity - fertility Based on available data the classification criteria are not met.

Reproductive toxicity -development


Specific target organ toxicity - single exposureSTOT - single exposure Not classified as a specific target organ toxicant after a single exposure.

Specific target organ toxicity - repeated exposureSTOT - repeated exposure Not classified as a specific target organ toxicant after repeated exposure.

Aspiration hazardAspiration hazard Based on available data the classification criteria are not met.

General information No specific health hazards known. The severity of the symptoms described will varydependent on the concentration and the length of exposure.





Route of entry Ingestion Inhalation Skin and/or eye contact

Target organs No specific target organs known.

SECTION 12: Ecological Information

Ecotoxicity Not considered to be a significant hazard due to the small quantities used. Not regarded asdangerous for the environment. However, large or frequent spills may have hazardous effectson the environment.

12.1. Toxicity

Toxicity Based on available data the classification criteria are not met.


Persistence and degradability The degradability of the product is not known.


6/8


Elution Buffer

Bioaccumulative potential No data available on bioaccumulation.



Mobility The product is water-soluble and may spread in water systems.


Results of PBT and vPvBassessment



Other adverse effects None known.

SECTION 13: Disposal considerations


General information The generation of waste should be minimised or avoided wherever possible. Reuse or recycleproducts wherever possible. This material and its container must be disposed of in a safeway. Disposal of this product, process solutions, residues and by-products should at all timescomply with the requirements of environmental protection and waste disposal legislation andany local authority requirements.

Disposal methods Dispose of surplus products and those that cannot be recycled via a licensed waste disposalcontractor. Waste packaging should be collected for reuse or recycling. Incineration or landfillshould only be considered when recycling is not feasible. Waste should not be disposed ofuntreated to the sewer unless fully compliant with the requirements of the local waterauthority.

SECTION 14: Transport information

General The product is not covered by international regulations on the transport of dangerous goods(IMDG, IATA, ADR/RID).

14.1. UN number

Not applicable.


Not applicable.



14.4. Packing group

Not applicable.


Environmentally hazardous substance/marine pollutant

No.


Not applicable.


7/8


Elution Buffer

Transport in bulk according toAnnex II of MARPOL 73/78and the IBC Code

Not applicable.

SECTION 15: Regulatory information


National regulations Health and Safety at Work etc. Act 1974 (as amended).The Carriage of Dangerous Goods and Use of Transportable Pressure EquipmentRegulations 2009 (SI 2009 No. 1348) (as amended) ["CDG 2009"].EH40/2005 Workplace exposure limits.

EU legislation Regulation (EC) No 1907/2006 of the European Parliament and of the Council of 18December 2006 concerning the Registration, Evaluation, Authorisation and Restriction ofChemicals (REACH) (as amended).Commission Regulation (EU) No 453/2010 of 20 May 2010.Regulation (EC) No 1272/2008 of the European Parliament and of the Council of 16December 2008 on classification, labelling and packaging of substances and mixtures (asamended).

15.2. Chemical safety assessment


SECTION 16: Other information

General information This product is considered to be a small package and is labelled according to the relevantprovisions of the legislation.

Training advice Read and follow manufacturer's recommendations. Only trained personnel should use thismaterial.

Revision comments This is first issue.

Revision date 12/08/2015

SDS number 3593

This information relates only to the specific material designated and may not be valid for such material used in combinationwith any other materials or in any process. Such information is, to the best of the company's knowledge and belief, accurateand reliable as of the date indicated. However, no warranty, guarantee or representation is made to its accuracy, reliability orcompleteness. It is the user's responsibility to satisfy himself as to the suitability of such information for his own particular use.

8/8

Revision Date 23/05/2014

SAFETY DATA SHEET

1D Adapter Mix

According to Regulation (EU) No 453/2010

SECTION 1: IDENTIFICATION OF THE SUBSTANCE/MIXTURE AND OF THE COMPANY/UNDERTAKING


Product name 1D Adapter Mix

Container size 50 μL


Identified uses Laboratory Reagent. For research purposes only.


Oxford Nanopore Technologies Ltd.Supplier

Edmund Cartwright House,

4 Robert Robinson Avenue,

Oxford Science Park,

Oxford, OX4 4GA

UK

T: +44 (0) 845 034 7900

F: +44 (0) 845 034 7901

W: www.nanoporetech.com

E: [email protected]


+44 (0) 845 034 7900 (Mon - Fri 09:00 - 17:00 h)

SECTION 2: HAZARDS IDENTIFICATION

2.1. Classification of the substance or mixture

Classification (EC 1272/2008)

Physical and Chemical Hazards Not classified.

Human health Not classified.

Environment Not classified.

Classification (1999/45/EEC) Not classified.

The Full Text for all R-Phrases and Hazard Statements are Displayed in Section 16.

2.2. Label elements

Label In Accordance With (EC) No. 1272/2008

No pictogram required.

Precautionary Statements

P280 Wear protective gloves, eye and face protection.

2.3. Other hazards


SECTION 3: COMPOSITION/INFORMATION ON INGREDIENTS

3.2. Mixtures

Composition Comments

None of the ingredients are required to be listed. This product does not contain any hazardous ingredients, or ingredients with national

workplace exposure limits.

SECTION 4: FIRST AID MEASURES

61 /

1D Adapter Mix


General information

Caution - substance not yet fully tested.

Inhalation

Move the exposed person to fresh air at once. Provide rest, warmth and fresh air. Get medical attention if any discomfort continues.

Ingestion

Do not induce vomiting. Rinse mouth thoroughly. Get medical attention if any discomfort continues.

Skin contact

Wash skin thoroughly with soap and water. Get medical attention promptly if symptoms occur after washing.

Eye contact

Make sure to remove any contact lenses from the eyes before rinsing. Immediately rinse with water. Get medical attention if any

discomfort continues.


Inhalation


Ingestion

May cause discomfort if swallowed.

Skin contact

Prolonged skin contact may cause redness and irritation.

Eye contact

Prolonged contact may cause redness and/or tearing.


Treat Symptomatically.

SECTION 5: FIREFIGHTING MEASURES


Extinguishing media

Extinguish with foam, carbon dioxide, dry powder or water fog.

Unsuitable extinguishing media



Hazardous combustion products

During fire, toxic gases (CO, CO2) are formed. Due to the small packaging, the risk of inhaling gases formed in a fire is minimal.

Unusual Fire & Explosion Hazards

No unusual fire or explosion hazards noted.

Specific hazards

No specific precautions due to the small quantities handled.


Special Fire Fighting Procedures

Avoid breathing fire vapours. If possible, fight fire from protected position.

Protective equipment for fire-fighters

Self contained breathing apparatus and full protective clothing must be worn in case of fire.

SECTION 6: ACCIDENTAL RELEASE MEASURES


For personal protection, see section 8.


Avoid discharge into drains, water courses or onto the ground.


Wear necessary protective equipment. Absorb spillage with suitable absorbent material. The product should not be dumped in nature but

collected and delivered according to agreement with the local authorities.


62 /

1D Adapter MixFor personal protection, see section 8. See section 11 for additional information on health hazards. For waste disposal, see section 13.

SECTION 7: HANDLING AND STORAGE


Read and follow manufacturer's recommendations. Avoid spilling, skin and eye contact. Good personal hygiene is necessary. Wash

hands and contaminated areas with water and soap before leaving the work site.


Store in tightly closed original container. Store at temperatures not exceeding 4°C.


The identified uses for this product are detailed in Section 1.2.

SECTION 8: EXPOSURE CONTROLS/PERSONAL PROTECTION


Ingredient Comments

No exposure limits noted for ingredient(s).


Engineering measures

Provide adequate ventilation.

Respiratory equipment

No specific recommendations.

Hand protection

For prolonged or repeated skin contact use suitable protective gloves. The most suitable glove must be chosen in consultation with the

gloves supplier, who can inform about the breakthrough time of the glove material.

Eye protection

Wear approved safety goggles. Provide eyewash station.

Hygiene measures

Wash hands after handling. When using do not eat, drink or smoke. Wash hands at the end of each work shift and before eating, smoking

and using the toilet.

Skin protection

Wear apron or protective clothing in case of splashes.

Environmental Exposure Controls

Keep container tightly sealed when not in use.

SECTION 9: PHYSICAL AND CHEMICAL PROPERTIES


Appearance Liquid

Colour Colourless.

Odour No characteristic odour.

Solubility Soluble in water.

Initial boiling point and boiling range

(°C)

~100 °C

Melting point (°C) ~ 0 °C

Relative density

Not available.

Bulk Density

Not available.

Vapour density (air=1)

Not available.

Vapour pressure

Not available.

Evaporation rate

Not available.

pH-Value, Conc. Solution

Not available.

63 /

1D Adapter MixViscosity

Not available.

Decomposition temperature (°C)

Not available.

Odour Threshold, Lower

Not available.

Odour Threshold, Upper

Not available.

Flash point (°C)

Not available.

Auto Ignition Temperature (°C)

Not available.

Flammability Limit - Lower(%)

Not available.

Flammability Limit - Upper(%)

Not available.

Partition Coefficient

(N-Octanol/Water)

Not available.

Explosive properties

Not considered to be explosive.

Oxidising properties

Does not meet the criteria for oxidising.


No information required.

SECTION 10: STABILITY AND REACTIVITY

10.1. Reactivity

No specific reactivity hazards associated with this product.


Stable under the prescribed storage conditions.


Not known.


Avoid excessive heat for prolonged periods of time.


Materials To Avoid

Strong oxidising substances. Strong reducing agents.


None at ambient temperatures.

SECTION 11: TOXICOLOGICAL INFORMATION


Acute toxicity:


Skin Corrosion/Irritation:


Serious eye damage/irritation:


Respiratory or skin sensitisation:


64 /

1D Adapter Mix

Germ cell mutagenicity:


Carcinogenicity:


Reproductive Toxicity:


Specific target organ toxicity - single exposure:

Not classified as a specific target organ toxicant after a single exposure.

Specific target organ toxicity - repeated exposure:

Not classified as a specific target organ toxicant after repeated exposure.

Aspiration hazard:


SECTION 12: ECOLOGICAL INFORMATION

Ecotoxicity



12.1. Toxicity

Acute Fish Toxicity

Not considered toxic to fish.


Degradability

The degradability of the product has not been stated.





Not available.


Mobility:

The product is soluble in water.




None known.

SECTION 13: DISPOSAL CONSIDERATIONS

General information

Avoid release to the environment.


Dispose of waste and residues in accordance with local authority requirements.

SECTION 14: TRANSPORT INFORMATION

General The product is not covered by international regulation on the transport of dangerous goods (IMDG, IATA,

ADR/RID).

14.1. UN number

Not applicable.

65 /

1D Adapter Mix


Not applicable.


Transport Labels


14.4. Packing group

Not applicable.


Environmentally Hazardous Substance/Marine Pollutant

No.


Not applicable.


Not applicable.

SECTION 15: REGULATORY INFORMATION


Approved Code Of Practice

Classification and Labelling of Substances and Preparations Dangerous for Supply. Safety Data Sheets for Substances and Preparations.

Guidance Notes

Workplace Exposure Limits EH40.

EU Legislation

Regulation (EC) No 1272/2008 of the European Parliament and of the Council of 16 December 2008 on classification, labelling and

packaging of substances and mixtures, amending and repealing Directives 67/548/EEC and 1999/45/EC, and amending Regulation (EC)

No 1907/2006 with amendments. Regulation (EC) No 1907/2006 of the European Parliament and of the Council of 18 December 2006

concerning the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH), establishing a European Chemicals

Agency, amending Directive 1999/45/EC and repealing Council Regulation (EEC) No 793/93 and Commission Regulation (EC) No 1488/94

as well as Council Directive 76/769/EEC and Commission Directives 91/155/EEC, 93/67/EEC, 93/105/EC and 2000/21/EC, including

amendments.

15.2. Chemical Safety Assessment


SECTION 16: OTHER INFORMATION

Revision Comments

This is first issue.

Revision Date 23/05/2014

Risk Phrases In Full

Not classified.NC

66 /


SAFETY DATA SHEETRunning Buffer FM




Product name Running Buffer FM









2.1. Classification of the substance or mixtureClassification (EC/1272/2008)Physical hazards Not Classified



2.2. Label elements


Precautionary statements P280 Wear protective gloves/ protective clothing/ eye protection/ face protection.


2.3. Other hazards



3.2. Mixtures

1/8


Running Buffer FM

Composition comments This product does not contain any hazardous ingredients, or ingredients with nationalworkplace exposure limits.





Ingestion Rinse mouth thoroughly with water. Give a few small glasses of water or milk to drink. Stop ifthe affected person feels sick as vomiting may be dangerous. Do not induce vomiting unlessunder the direction of medical personnel.























2/8


Running Buffer FM







Personal precautions Keep unnecessary and unprotected personnel away from the spillage. Wear protectiveclothing as described in Section 8 of this safety data sheet. Wash thoroughly after dealing witha spillage.




Methods for cleaning up Wear protective clothing as described in Section 8 of this safety data sheet. Clear up spillsimmediately and dispose of waste safely. Reuse or recycle products wherever possible. If theproduct is soluble in water, dilute the spillage with water and mop it up. Dispose of waste tolicensed waste disposal site in accordance with the requirements of the local Waste DisposalAuthority. Wash thoroughly after dealing with a spillage.





Usage precautions Read and follow manufacturer's recommendations. Wear protective clothing as described inSection 8 of this safety data sheet. Keep away from food, drink and animal feeding stuffs.Handle all packages and containers carefully to minimise spills. Keep container tightly sealedwhen not in use.




Storage precautions Store in accordance with local regulations.








3/8


Running Buffer FM





Hand protection Chemical-resistant, impervious gloves complying with an approved standard should be worn ifa risk assessment indicates skin contact is possible. The most suitable glove should bechosen in consultation with the glove supplier/manufacturer, who can provide informationabout the breakthrough time of the glove material. To protect hands from chemicals, glovesshould comply with European Standard EN374. Considering the data specified by the glovemanufacturer, check during use that the gloves are retaining their protective properties andchange them as soon as any deterioration is detected.









Appearance Liquid.

Colour Colourless.

Odour Odourless.


pH Not available.






Not available.


4/8


Running Buffer FM













10.1. Reactivity








Conditions to avoid There are no known conditions that are likely to result in a hazardous situation.


Materials to avoid No specific material or group of materials is likely to react with the product to produce ahazardous situation.










Skin corrosion/irritation

5/8


Running Buffer FM

Animal data Based on available data the classification criteria are not met.

Serious eye damage/irritationSerious eye damage/irritation Based on available data the classification criteria are not met.





















12.1. Toxicity




6/8






Mobility

Running Buffer FM


Not available.

The product is water-soluble and may spread in water systems.












14.1. UN number

Not applicable.


Not applicable.



14.4. Packing group

Not applicable.


Environmentally hazardous substance/marine pollutantNo.


Not applicable.

14.7. Transport in bulk according to Annex II of MARPOL and the IBC Code

7/8


Running Buffer FM


Not applicable.



National regulations The Carriage of Dangerous Goods and Use of Transportable Pressure EquipmentRegulations 2009 (SI 2009 No. 1348) (as amended) ["CDG 2009"].EH40/2005 Workplace exposure limits.






Training advice Read and follow manufacturer's recommendations.



SDS number 4225


8/8


SAFETY DATA SHEETDNA CS




Product name DNA CS













2.2. Label elements




2.3. Other hazards



3.2. Mixtures

1/8


DNA CS






Ingestion Rinse mouth thoroughly with water. Give a few small glasses of water or milk to drink. Stop ifthe affected person feels sick as vomiting may be dangerous. Do not induce vomiting unlessunder the direction of medical personnel. If vomiting occurs, the head should be kept low sothat vomit does not enter the lungs. Never give anything by mouth to an unconscious person.







Ingestion Gastrointestinal symptoms, including upset stomach. Fumes from the stomach contents maybe inhaled, resulting in the same symptoms as inhalation.
















2/8


DNA CS







Personal precautions No action shall be taken without appropriate training or involving any personal risk. Keepunnecessary and unprotected personnel away from the spillage. Wear protective clothing asdescribed in Section 8 of this safety data sheet. Wash thoroughly after dealing with a spillage.




Methods for cleaning up Wear protective clothing as described in Section 8 of this safety data sheet. Reuse or recycleproducts wherever possible. If the product is soluble in water, dilute the spillage with waterand mop it up. Flush contaminated area with plenty of water. Dispose of waste to licensedwaste disposal site in accordance with the requirements of the local Waste Disposal Authority.Wash thoroughly after dealing with a spillage.

















3/8


DNA CS





Hand protection Chemical-resistant, impervious gloves complying with an approved standard should be worn ifa risk assessment indicates skin contact is possible. The most suitable glove should bechosen in consultation with the glove supplier/manufacturer, who can provide informationabout the breakthrough time of the glove material. To protect hands from chemicals, glovesshould comply with European Standard EN374. Considering the data specified by the glovemanufacturer, check during use that the gloves are retaining their protective properties andchange them as soon as any deterioration is detected. Frequent changes are recommended.









Appearance Liquid.

Colour Colourless.

Odour Odourless.


pH Not available.






Not available.


4/8


DNA CS













10.1. Reactivity





















5/8


DNA CS























12.1. Toxicity



6/8


DNA CS


















14.1. UN number

Not applicable.


Not applicable.



14.4. Packing group

Not applicable.




Not applicable.

7/8


DNA CS



Not applicable.












SDS number 4235


8/8


SAFETY DATA SHEETPCR Adapters




Product name PCR Adapters













2.2. Label elements




2.3. Other hazards



3.2. Mixtures

1/8


PCR Adapters





























2/8


PCR Adapters




























3/8


PCR Adapters














Appearance Liquid.

Colour Colourless.

Odour Odourless.


pH Not available.






Not available.


4/8


PCR Adapters













10.1. Reactivity





















5/8


PCR Adapters























12.1. Toxicity



6/8


PCR Adapters


















14.1. UN number

Not applicable.


Not applicable.



14.4. Packing group

Not applicable.




Not applicable.

7/8


PCR Adapters



Not applicable.












SDS number 4238


8/8


SAFETY DATA SHEETAdapter Bead Binding Buffer

According to Regulation (EC) No 1907/2006, Annex II, as amended., COMMISSION REGULATION (EU)2015/830 of 28 May 2015.



Product name Adapter Bead Binding Buffer









2.1. Classification of the substance or mixtureClassification (EC 1272/2008)Physical hazards Not Classified



2.2. Label elements


Precautionary statements P280 Wear protective clothing, gloves, eye and face protection.


2.3. Other hazards



3.2. Mixtures

1/8


Adapter Bead Binding Buffer

Composition comments No classified ingredients, or those having occupational exposure limits, present above thelevels of disclosure.





Ingestion Rinse mouth thoroughly with water. Give a few small glasses of water or milk to drink. Stop ifthe affected person feels sick as vomiting may be dangerous. Do not induce vomiting unlessunder the direction of medical personnel. If vomiting occurs, the head should be kept low sothat vomit does not enter the lungs. Never give anything by mouth to an unconscious person.Maintain an open airway. Loosen tight clothing such as collar, tie or belt.






















2/8










Personal precautions No action shall be taken without appropriate training or involving any personal risk. Keepunnecessary and unprotected personnel away from the spillage. Wear protective clothing asdescribed in Section 8 of this safety data sheet. Follow precautions for safe handlingdescribed in this safety data sheet. Wash thoroughly after dealing with a spillage.




Methods for cleaning up Wear protective clothing as described in Section 8 of this safety data sheet. If the product issoluble in water, dilute the spillage with water and mop it up. Flush contaminated area withplenty of water. Wash thoroughly after dealing with a spillage. Dispose of waste to licensedwaste disposal site in accordance with the requirements of the local Waste Disposal Authority.
















3/8

















Appearance Liquid.

Colour Colourless.

Odour Odourless.


pH Not available.






Not available.

4/8
















10.1. Reactivity




















5/8



Skin corrosion/irritationAnimal data Based on available data the classification criteria are not met.






















12.1. Toxicity


6/8





















14.1. UN number

Not applicable.


Not applicable.



14.4. Packing group

Not applicable.




Not applicable.

7/8





Not applicable.




EU legislation Regulation (EC) No 1907/2006 of the European Parliament and of the Council of 18December 2006 concerning the Registration, Evaluation, Authorisation and Restriction ofChemicals (REACH) (as amended).COMMISSION REGULATION (EU) 2015/830 of 28 May 2015.Regulation (EC) No 1272/2008 of the European Parliament and of the Council of 16December 2008 on classification, labelling and packaging of substances and mixtures (asamended).








SDS number 4653


8/8


SAFETY DATA SHEETPCR Primers




Product name PCR Primers













2.2. Label elements




2.3. Other hazards



3.2. Mixtures

1/8


PCR Primers





























2/8


PCR Primers




























3/8


PCR Primers














Appearance Liquid.

Colour Colourless.

Odour Odourless.


pH Not available.






Not available.


4/8


PCR Primers













10.1. Reactivity





















5/8


PCR Primers























12.1. Toxicity



6/8


PCR Primers


















14.1. UN number

Not applicable.


Not applicable.



14.4. Packing group

Not applicable.




Not applicable.

7/8


PCR Primers



Not applicable.












SDS number 4239


8/8

sqk-lsk10 sequencing w 1d - nanopore · 2016-10-10 · lambda dna 6.3. methods and material for...

Documents