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Medical Electronic Systems SQA-Vb NEW TECHNOLOGY for the Bull Industry

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Page 1: SQA-Vb Presentation

Medical Electronic Systems

SQA-VbNEW TECHNOLOGYfor the Bull Industry

Page 2: SQA-Vb Presentation

Medical Electronic Systems

Medical Electronic Systems

• Established in 1993 in Israel.

• 2005: Offices established in Vienna and Los Angeles

• Technology Focus: Medical ElectronicsElectro-optical engineering

Computer algorithms

Video microscopy

• Commercialized automated semen analysis technology for both human and animal applications (SQA – Sperm Quality Analyzer)

• Installed base ~ 2,500 systems worldwide

Page 3: SQA-Vb Presentation

Medical Electronic Systems

HUMAN SPERM QUALITY ANALYZERS

SQA II CP

SQA-V

Page 4: SQA-Vb Presentation

Medical Electronic Systems

ANIMAL SPERM QUALITY ANALYZERS

SQA-VtTURKEY

SQA-VpPIG

SQA-VbBULL

Page 5: SQA-Vb Presentation

Medical Electronic Systems

SOFTWARE PRODUCTS

V-Sperm™ Video and Data Management Software (both English and Russian)

T-Sperm™Turkey Video, Data and Flock Management Software

B-Sperm™Bull Video, Herd and Data Management and Dosing Software

P-Sperm™Pig Video, Herd and Data Management and Dosing Software

Qwik-LinkTM LIS interface for information transfer to a centralized database

Page 6: SQA-Vb Presentation

Medical Electronic Systems

Qwik-CheckTM Kits

Page 7: SQA-Vb Presentation

Medical Electronic Systems

Why Automate Semen Analysis?

Manual Semen Analysis is:

• Time consuming – 70 minutes if done correctly following WHO standards

• Highly variable – Errors counting highly motile cells. Some parameters such as morphology are highly subjective.

• Labor intensive and requires special training –Preparation of stained slides, counting> 200 moving cells, etc.

• Not standardized – high variability between labs and technicians

Page 8: SQA-Vb Presentation

Medical Electronic Systems

SQA V Advantages

• Results in 70 seconds

• Fully automated

• Accurate, repeatable results

• User friendly interactive screens

• Counts thousands of cells automatically compared to hundreds of cells manually

Page 9: SQA-Vb Presentation

Medical Electronic Systems

CASA Limitations

• Expensive and requires a variety of modules

• Requires considerable lab space (compared to the SQA-V)

• Settings must be set prior to each analysis

• Parameter setting are subjective

• Requires extensive training and re-validation of technician competency

Page 10: SQA-Vb Presentation

Medical Electronic Systems

The SQA Vb: Overview

Applications for testing bull semen:

• Dairy Application for AI dosing and QC - In conjunction with B-Sperm software:

• Fresh samples can be tested and dosing calculations performed based on total, motile or progressive motile cell concentration

• Frozen semen samples can be evaluated for quality after straw preparation and prior to insemination

• BSE – A system for performing BREEDING SOUNDNESS EVALUATION in the field

Page 11: SQA-Vb Presentation

Medical Electronic Systems

SQA-Vb: System Components

• SQA-Vb

• B-Sperm Management Software

• Test Kit with I-button and 50 testing capillaries

• Cleaning Kit

• QwikCheck™-beads for QC

Page 12: SQA-Vb Presentation

Medical Electronic Systems

SQA-Vb Components

Data management software

Disposable testing capillary (10 uses)

Diluent dispenser and pipette

QC Beads SQA-V (Vb) Cleaning Kit

Page 13: SQA-Vb Presentation

Medical Electronic Systems

SQA-Vb Overview

• The SQA-Vb automatically measures the following parameters in less than 1 minute:

% MorphologyProgressive Motile Sperm Concentration M/ml

Total Progressive Motile # Sperm/EjaculateProgressive Motility %

Total Motile # Sperm/EjaculateMotility %

Total # Sperm/EjaculateMotile Sperm Concentration M/ml

Velocity microns/secConcentration M/ml

Semen Parameters

Page 14: SQA-Vb Presentation

Medical Electronic Systems

Running FRESH Samples for DOSING and BSE

Page 15: SQA-Vb Presentation

Medical Electronic Systems

Dairy and BSE – Running FRESH Samples

ENTER SAMPLE DATA: FRESH

TEMP. 26.5 CHERD # 1BULL ID: 7254 SEMEN VOLUME: 6.5 mlFRESHE SAMPLE #: 12254358

PRESS ENTER TO CONTINUE

SQA-Vb TESTING SCREENS

FRESH SPECIMEN: SAMPLE PREPERATION

1. SEMEN: 100 microliters2. DILUENT: 2.0 ml3. MIX SAMPLE THOROUGHLY4. FILL AND CLEAN CAPILLARY

INSERT CAPILLARY INTO CHAMBER

Dispense 2ml testing medium

STEP #1

Aspirate 100µl semen using a pipette

STEP # 2

Mix the semen sampleand the testing medium

STEP #3

FRESH SAMPLE PREPARATION

Aspirate the sample into the testing capillary

STEP # 4

Page 16: SQA-Vb Presentation

Medical Electronic Systems

Dairy and BSE : Running FRESH Samples

TEST RESULTS: FRESH SAMPLECONC. 3 32.6 M/ml MSC 259.1M/mlMOTILITY 77.9 % PMSC 183.9 M/ml PR. MOT. 55.3 % VELOC. 32 mic/sec% Morphology 55 %

TOTALS PER EJACULATESPERM # 0.67 Bil MOT. SPERM 0.52 Bil PR. SPERM 0.37 Bil

SQA-Vb TESTING SCREENS

% MorphologyProgressive Motile Sperm Concentration M/ml

Total Progressive Motile # Sperm/EjaculateProgressive Motility %

Total Motile # Sperm/EjaculateMotility %

Total # Sperm/EjaculateMotile Sperm Concentration M/ml

Velocity microns/secConcentration M/ml

Semen Parameters: AI Dosing and BSE

Page 17: SQA-Vb Presentation

Medical Electronic Systems

Dairy: Dosing in B-Sperm

Page 18: SQA-Vb Presentation

Medical Electronic Systems

B-Sperm Dosing Report

Page 19: SQA-Vb Presentation

Medical Electronic Systems

Running FROZEN Samples for Quality Control

Page 20: SQA-Vb Presentation

Medical Electronic Systems

QC Sample Preparation & Semen Analysis

FROZEN SAMPLE PREPARATION

Express the semen sample from the straw into a plastic container or dissolve a frozen sperm tablet in a pre-heated media. Use 200 µl for testing.

Progressive Motile # Sperm/Ejaculate

Motile # Sperm/Ejaculate

Velocity microns/second

Progressive Motile Sperm Concentration M/ml

Motile and Total Sperm Concentration M/ml

Semen Parameters: QC

QC can be performed before and after freezing AI samples

Page 21: SQA-Vb Presentation

Medical Electronic Systems

B-Sperm QC FROZEN Screen

Page 22: SQA-Vb Presentation

Medical Electronic Systems

B-Sperm Software

• B-Sperm software is provided with each SQA-Vb in order to:

• Manage and save herd and bull data to the PC

• Provide on-line dosing calculations

• View specimens on the PC screen

• In the BSE mode the user can:

• View test results and analyze herd data

• Sort bulls based on the quality of their sperm

• Import data from the SQA to the PC at the end of the day

• In the Dosing mode the user can:

• Calculate sample dilution parameters based on test results (Total, Motile or Progressive Motile Sperm Concentration)

• Divide fresh semen samples into AI doses based on on-line dosing calculations

Page 23: SQA-Vb Presentation

Medical Electronic Systems

SQA-Vb Technology and Performance Data

Page 24: SQA-Vb Presentation

Medical Electronic Systems

SQA-Vb Technology

• The SQA-Vb testing capillary is inserted into the optical block and testing begins.

• Concentration is measured by analyzing millions of sperm cells in the thick section of the SQA-V testing capillary: A very specific wavelength of light is absorbed by the sperm cells.

• A detector measures the amount of light absorbed by the sperm cells and coverts this value to optical density (OD).

• “OD” is translated into sperm concentration by a microprocessor and proprietary MES algorithms.

• Motility is measured by analyzing tens of thousands of sperm cells in the thin section of the SQA-V capillary: Motile cells pass through a light source creating disturbances in the beam of light.

• A motility detector converts these light disturbances into electrical signals (“peaks and valleys”) and transmits them to a converter which translates them into digital form.

• These electronic signals are analyzed by the SQA software and proprietary algorithms and translated into sperm motility parameters.

SQA-Vb Optical Block

LED

A/D Conv.

A/D Conv.

Micro

Processor

M.E.S.ProprietaryAlgorithms

SemenParameters

LED

Display Printer Archive CPU Keypad

Density Detector

Motility Detector

Page 25: SQA-Vb Presentation

Medical Electronic Systems

SQA-Vb: Dynamic Range

0-95

0-1800

PMSCM/ml

-0-700-1000-950-100Frozen

0-1000-1300-19000-950-2000Fresh

Morphology%

Velocity mic/sec

MSCM/ml

Motility%

Conc.M/ml

SampleType

SQA-Vb Dynamic Range for FRESH and FROZEN Bull Semen

Page 26: SQA-Vb Presentation

Medical Electronic Systems

SQA-Vb: Performance Claims

0.8

5%

Motility%

< 2.5Inter-device Variability (CV, %)< 0.01Intra-device Variability (CV, %)

Repeatability (QC material)

0.710.750.80.90.9Accuracy (correlation to manual data)

5%10%-7%3%Precision (CV, %)

Morphology %Velocity mic/sec

PMSCM/ml

MSCM/ml

Conc.M/ml

Claims

FRESH BULL SEMEN

FROZEN BULL SEMEN (QC)

4%-7%Precision (CV, %)

0.850.70.8Accuracy (correlation to manual data)

Velocity mic/sec

PMSCM/ml

MSCM/mlClaims

Page 27: SQA-Vb Presentation

Medical Electronic Systems

SQA-Vb Comparison to CASA

Page 28: SQA-Vb Presentation

Medical Electronic Systems

SQA-Vb vs. CASA

Intra-device ≤ 0.01

Inter-device ≤ 2.5 Repeatability using QC material (CV, %)

Concentration: 3%

Motility: 5%Precision (CV, %) Inconsistent

Concentration: 0.9

Motility: 0.8Accuracy (correlation to manual method)

Partial (a lot of settings and adjustments)

FullAutomation

Variable depending on sample qualityConstantDilution rate

200-400 cells (setting dependent)

Concentration channel: Millions

Motility channel: Thousands# Spermatozoa tested

5-10 µl100 µl – Fresh semen

20 µl – Frozen semen

Sample volume for testing

CASASQA-VbParameter

Comparison Table: SQA-Vb vs. CASA

Page 29: SQA-Vb Presentation

Medical Electronic Systems

The SQA Vb: Summary

• BSE – A stand-alone system for performing BREEDING SOUNDNESS EVALUATION in the field

• Dosing QC/AI- In conjunction with B-Sperm software:

• Fresh samples can be tested and dosing calculations performed based on total, motile or progressive motile sperm concentration

• Frozen semen samples can be evaluated for quality prior to insemination

SQA-Vb: Automated test results in less than one minute!

Page 30: SQA-Vb Presentation

Medical Electronic Systems

SQA-VbNEW TECHNOLOGY

in the Cattle Industry