sqa-vb presentation
TRANSCRIPT
Medical Electronic Systems
SQA-VbNEW TECHNOLOGYfor the Bull Industry
Medical Electronic Systems
Medical Electronic Systems
• Established in 1993 in Israel.
• 2005: Offices established in Vienna and Los Angeles
• Technology Focus: Medical ElectronicsElectro-optical engineering
Computer algorithms
Video microscopy
• Commercialized automated semen analysis technology for both human and animal applications (SQA – Sperm Quality Analyzer)
• Installed base ~ 2,500 systems worldwide
Medical Electronic Systems
HUMAN SPERM QUALITY ANALYZERS
SQA II CP
SQA-V
Medical Electronic Systems
ANIMAL SPERM QUALITY ANALYZERS
SQA-VtTURKEY
SQA-VpPIG
SQA-VbBULL
Medical Electronic Systems
SOFTWARE PRODUCTS
V-Sperm™ Video and Data Management Software (both English and Russian)
T-Sperm™Turkey Video, Data and Flock Management Software
B-Sperm™Bull Video, Herd and Data Management and Dosing Software
P-Sperm™Pig Video, Herd and Data Management and Dosing Software
Qwik-LinkTM LIS interface for information transfer to a centralized database
Medical Electronic Systems
Qwik-CheckTM Kits
Medical Electronic Systems
Why Automate Semen Analysis?
Manual Semen Analysis is:
• Time consuming – 70 minutes if done correctly following WHO standards
• Highly variable – Errors counting highly motile cells. Some parameters such as morphology are highly subjective.
• Labor intensive and requires special training –Preparation of stained slides, counting> 200 moving cells, etc.
• Not standardized – high variability between labs and technicians
Medical Electronic Systems
SQA V Advantages
• Results in 70 seconds
• Fully automated
• Accurate, repeatable results
• User friendly interactive screens
• Counts thousands of cells automatically compared to hundreds of cells manually
Medical Electronic Systems
CASA Limitations
• Expensive and requires a variety of modules
• Requires considerable lab space (compared to the SQA-V)
• Settings must be set prior to each analysis
• Parameter setting are subjective
• Requires extensive training and re-validation of technician competency
Medical Electronic Systems
The SQA Vb: Overview
Applications for testing bull semen:
• Dairy Application for AI dosing and QC - In conjunction with B-Sperm software:
• Fresh samples can be tested and dosing calculations performed based on total, motile or progressive motile cell concentration
• Frozen semen samples can be evaluated for quality after straw preparation and prior to insemination
• BSE – A system for performing BREEDING SOUNDNESS EVALUATION in the field
Medical Electronic Systems
SQA-Vb: System Components
• SQA-Vb
• B-Sperm Management Software
• Test Kit with I-button and 50 testing capillaries
• Cleaning Kit
• QwikCheck™-beads for QC
Medical Electronic Systems
SQA-Vb Components
Data management software
Disposable testing capillary (10 uses)
Diluent dispenser and pipette
QC Beads SQA-V (Vb) Cleaning Kit
Medical Electronic Systems
SQA-Vb Overview
• The SQA-Vb automatically measures the following parameters in less than 1 minute:
% MorphologyProgressive Motile Sperm Concentration M/ml
Total Progressive Motile # Sperm/EjaculateProgressive Motility %
Total Motile # Sperm/EjaculateMotility %
Total # Sperm/EjaculateMotile Sperm Concentration M/ml
Velocity microns/secConcentration M/ml
Semen Parameters
Medical Electronic Systems
Running FRESH Samples for DOSING and BSE
Medical Electronic Systems
Dairy and BSE – Running FRESH Samples
ENTER SAMPLE DATA: FRESH
TEMP. 26.5 CHERD # 1BULL ID: 7254 SEMEN VOLUME: 6.5 mlFRESHE SAMPLE #: 12254358
PRESS ENTER TO CONTINUE
SQA-Vb TESTING SCREENS
FRESH SPECIMEN: SAMPLE PREPERATION
1. SEMEN: 100 microliters2. DILUENT: 2.0 ml3. MIX SAMPLE THOROUGHLY4. FILL AND CLEAN CAPILLARY
INSERT CAPILLARY INTO CHAMBER
Dispense 2ml testing medium
STEP #1
Aspirate 100µl semen using a pipette
STEP # 2
Mix the semen sampleand the testing medium
STEP #3
FRESH SAMPLE PREPARATION
Aspirate the sample into the testing capillary
STEP # 4
Medical Electronic Systems
Dairy and BSE : Running FRESH Samples
TEST RESULTS: FRESH SAMPLECONC. 3 32.6 M/ml MSC 259.1M/mlMOTILITY 77.9 % PMSC 183.9 M/ml PR. MOT. 55.3 % VELOC. 32 mic/sec% Morphology 55 %
TOTALS PER EJACULATESPERM # 0.67 Bil MOT. SPERM 0.52 Bil PR. SPERM 0.37 Bil
SQA-Vb TESTING SCREENS
% MorphologyProgressive Motile Sperm Concentration M/ml
Total Progressive Motile # Sperm/EjaculateProgressive Motility %
Total Motile # Sperm/EjaculateMotility %
Total # Sperm/EjaculateMotile Sperm Concentration M/ml
Velocity microns/secConcentration M/ml
Semen Parameters: AI Dosing and BSE
Medical Electronic Systems
Dairy: Dosing in B-Sperm
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B-Sperm Dosing Report
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Running FROZEN Samples for Quality Control
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QC Sample Preparation & Semen Analysis
FROZEN SAMPLE PREPARATION
Express the semen sample from the straw into a plastic container or dissolve a frozen sperm tablet in a pre-heated media. Use 200 µl for testing.
Progressive Motile # Sperm/Ejaculate
Motile # Sperm/Ejaculate
Velocity microns/second
Progressive Motile Sperm Concentration M/ml
Motile and Total Sperm Concentration M/ml
Semen Parameters: QC
QC can be performed before and after freezing AI samples
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B-Sperm QC FROZEN Screen
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B-Sperm Software
• B-Sperm software is provided with each SQA-Vb in order to:
• Manage and save herd and bull data to the PC
• Provide on-line dosing calculations
• View specimens on the PC screen
• In the BSE mode the user can:
• View test results and analyze herd data
• Sort bulls based on the quality of their sperm
• Import data from the SQA to the PC at the end of the day
• In the Dosing mode the user can:
• Calculate sample dilution parameters based on test results (Total, Motile or Progressive Motile Sperm Concentration)
• Divide fresh semen samples into AI doses based on on-line dosing calculations
Medical Electronic Systems
SQA-Vb Technology and Performance Data
Medical Electronic Systems
SQA-Vb Technology
• The SQA-Vb testing capillary is inserted into the optical block and testing begins.
• Concentration is measured by analyzing millions of sperm cells in the thick section of the SQA-V testing capillary: A very specific wavelength of light is absorbed by the sperm cells.
• A detector measures the amount of light absorbed by the sperm cells and coverts this value to optical density (OD).
• “OD” is translated into sperm concentration by a microprocessor and proprietary MES algorithms.
• Motility is measured by analyzing tens of thousands of sperm cells in the thin section of the SQA-V capillary: Motile cells pass through a light source creating disturbances in the beam of light.
• A motility detector converts these light disturbances into electrical signals (“peaks and valleys”) and transmits them to a converter which translates them into digital form.
• These electronic signals are analyzed by the SQA software and proprietary algorithms and translated into sperm motility parameters.
SQA-Vb Optical Block
LED
A/D Conv.
A/D Conv.
Micro
Processor
M.E.S.ProprietaryAlgorithms
SemenParameters
LED
Display Printer Archive CPU Keypad
Density Detector
Motility Detector
Medical Electronic Systems
SQA-Vb: Dynamic Range
0-95
0-1800
PMSCM/ml
-0-700-1000-950-100Frozen
0-1000-1300-19000-950-2000Fresh
Morphology%
Velocity mic/sec
MSCM/ml
Motility%
Conc.M/ml
SampleType
SQA-Vb Dynamic Range for FRESH and FROZEN Bull Semen
Medical Electronic Systems
SQA-Vb: Performance Claims
0.8
5%
Motility%
< 2.5Inter-device Variability (CV, %)< 0.01Intra-device Variability (CV, %)
Repeatability (QC material)
0.710.750.80.90.9Accuracy (correlation to manual data)
5%10%-7%3%Precision (CV, %)
Morphology %Velocity mic/sec
PMSCM/ml
MSCM/ml
Conc.M/ml
Claims
FRESH BULL SEMEN
FROZEN BULL SEMEN (QC)
4%-7%Precision (CV, %)
0.850.70.8Accuracy (correlation to manual data)
Velocity mic/sec
PMSCM/ml
MSCM/mlClaims
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SQA-Vb Comparison to CASA
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SQA-Vb vs. CASA
Intra-device ≤ 0.01
Inter-device ≤ 2.5 Repeatability using QC material (CV, %)
Concentration: 3%
Motility: 5%Precision (CV, %) Inconsistent
Concentration: 0.9
Motility: 0.8Accuracy (correlation to manual method)
Partial (a lot of settings and adjustments)
FullAutomation
Variable depending on sample qualityConstantDilution rate
200-400 cells (setting dependent)
Concentration channel: Millions
Motility channel: Thousands# Spermatozoa tested
5-10 µl100 µl – Fresh semen
20 µl – Frozen semen
Sample volume for testing
CASASQA-VbParameter
Comparison Table: SQA-Vb vs. CASA
Medical Electronic Systems
The SQA Vb: Summary
• BSE – A stand-alone system for performing BREEDING SOUNDNESS EVALUATION in the field
• Dosing QC/AI- In conjunction with B-Sperm software:
• Fresh samples can be tested and dosing calculations performed based on total, motile or progressive motile sperm concentration
• Frozen semen samples can be evaluated for quality prior to insemination
SQA-Vb: Automated test results in less than one minute!
Medical Electronic Systems
SQA-VbNEW TECHNOLOGY
in the Cattle Industry