signaling by serine/ threonine kinase receptors

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Signaling by Serine/Threonine Kinase Receptors

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Signaling by Serine/ Threonine Kinase Receptors. Major Classes of Protein Ser/ Thr Kinases (not a complete list). 2 nd -Messenger-dependent protein kinases cAMP -dependent protein kinase cGMP -dependent protein kinase Ca 2+ /CaM-dependent protein kinases Protein kinase C - PowerPoint PPT Presentation

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Page 1: Signaling by Serine/ Threonine Kinase  Receptors

Signaling by Serine/Threonine Kinase Receptors

Page 2: Signaling by Serine/ Threonine Kinase  Receptors

Major Classes of Protein Ser/Thr Kinases(not a complete list)

• 2nd-Messenger-dependent protein kinasescAMP-dependent protein kinasecGMP-dependent protein kinaseCa2+/CaM-dependent protein kinases

Protein kinase C• 2nd-Messenger-independent protein kinases

MAPK kinase cascade and target kinasesRaf, MEK kinases, MEKs, SEKs, ERKs, JNKs, SAPKs, RSKs

Page 3: Signaling by Serine/ Threonine Kinase  Receptors

Major Classes of Protein Ser/Thr Kinases (cont’d)

• Cyclin dependent kinases (CDKs) and CDK regulating kinasesCdc-2, CAK, CAK kinase

• GPCR kinasesGRK2 (βARK1), GRK3 (βARK2), GRK5, GRK6

• P21-activated kinases (PAK)• Kinases involved in cytoskeletal organization and

development ROCK/Rho kinase

• Transmembrane receptor protein ser/thr kinasesTGFβ receptor protein kinase

• Casein kinasesCK1, CK2

Page 4: Signaling by Serine/ Threonine Kinase  Receptors

Receptors with intrinsic serine/threonine kinase activity

Page 5: Signaling by Serine/ Threonine Kinase  Receptors
Page 6: Signaling by Serine/ Threonine Kinase  Receptors

Smad 6/7 are inhibitory - they act as decoys and occupy the sites that would normally be utilized by other activating Smads.

Other proteins such as noggin and chordin bind BMPs and inhibit their ability to activate receptors

Follistatin inhibits signaling via activins

Page 7: Signaling by Serine/ Threonine Kinase  Receptors

Ser/Thr Protein Kinases

2 Major Types:1. 2nd-messenger-dependent protein kinases.2. 2nd-messenger-independent protein kinases.

Page 8: Signaling by Serine/ Threonine Kinase  Receptors

2nd-Messenger-Dependent Protein Kinases

All kinases in this category share common design:Several functional domains that can reside on the

same pp chain or on separate ones.Each kinase has a catalytic domain (intrinsically active),

which is kept inactive by a regulatory domain.Regulatory domain have auto-inhibitory regions and

binding sites for 2nd messengers.Interaction with the 2nd messenger dissociates the

auto-inhibitory site from the cat domain dis-inhibiting it.

Additional regions of the kinases may be responsible for oligomerization or for targeting the kinases to distinct cellular locations.

Page 9: Signaling by Serine/ Threonine Kinase  Receptors

The function of cAMPTargeting PKA (cyclic-AMP-dependent protein

kinase A)

PKA

Page 10: Signaling by Serine/ Threonine Kinase  Receptors
Page 11: Signaling by Serine/ Threonine Kinase  Receptors

Schematic view ofthe different domainsof 2nd-messenger-dependent proteinkinases. The differentkinases contain a regulatory domainthat encodes specialized functionaldomains and a conserved cat domainkept inactive by the auto-inhibitory region (black).

Page 12: Signaling by Serine/ Threonine Kinase  Receptors

PKA• Binding affinities of reg subunits to cAMP:

RIIβ < RIIα < RIα < RIβNecessary for decoding the cAMP signals that

differ in intensity and duration:PKAI activated transiently by weak cAMP signalsPKAII activated by persistent and strong cAMP

levels.RIα may be protective against proteolytic

degradation (as in, say, when one of the other subunits are removed (gene targeting)).

Page 13: Signaling by Serine/ Threonine Kinase  Receptors

Ca2+/CaM kinases (next slide)

(A) Domains and holoenzyme structure of CaMKII.(B) Mechanism of autophosphorylation.Thr286.Displacement of the autoinhibitory domain by

calmodulin exposes T286, which can then be phosphorylated if its proximate neighbor is active.

(C) Trapping of calmodulin to the CaMKII subunits increases the P(autophosphorylation) during successive Ca2+ spikes at high frequency.

Page 14: Signaling by Serine/ Threonine Kinase  Receptors
Page 15: Signaling by Serine/ Threonine Kinase  Receptors

Ca2+/CaM kinases

Autophosphorylation of Thr286/287 has 2 consequences:1. Calmodulin remains bound to the phosphorylated subunit for

extended periods of time even at low [Ca2+] (trapped state) because the autophosphorylation greatly decreases the calmodulin dissociation.

2. Autophosphorylated α and β subunits are rendered Ca/CaM-independent (autonomous), but still retain substantial kinase activity.

Both these consequences (calmodulin trapping and autonomy) allow the phosphorylated kinase subunits to remain active beyond the limited duration of a Ca2+ spike.

Transgenic mice carrying Thr286A1a point mutation in αCaMKII do not exhibit LTP.

NMDAR subunits (NR2B) remain active even after dissociation of Ca2+/CaM.

Page 16: Signaling by Serine/ Threonine Kinase  Receptors

PKC

Family consists of at least 10 structurally related phospholipid-dependent protein kinases.

Most are expressed in brain.All isozymes grouped into 3 subclasses according to their

regulatory properties (conferred by specific domains of the protein):

“Conventional”: (cPKCs; α, βI, βII, γ) are regulated by Ca2+, DAG, or phorbol esters and PS.

“Novel”: (nPKCs; δ, ε, θ, η) activated by DAG or phorbol esters and PS, but are Ca2+-independent.

“Atypical”: (aPKC; μ) unresponsive to DAG, Ca2+, or phorbol esters.

Page 17: Signaling by Serine/ Threonine Kinase  Receptors

PKC

Refer to Schematic view of PKC (earlier slide):Single polypeptide chain = N-term regulatory

domain and C-term cat (kinase) domain.Regulatory: auto-inhibitory or pseudo-substrate

site and 1-2 membrane targeting motifs (C1 and C2).

C1 binds phorbol esters and DAG.C2 binds acidic phospholipids and Ca2+ (in the

cPKCs).

Page 18: Signaling by Serine/ Threonine Kinase  Receptors

PKC

Regulation of PKC isozymes: requires removal of the auto-inhibitory pseudo-substrate from the active site (high specific binding of DAG and PS to the C1 and C2 domains) conformational Δ

This allows translocation of PKCs from cytoplasm to the membrane enzyme is maximally activated.

But, both phosphorylation of PKCs and specific protein-protein interactions are also important for their activation.

Page 19: Signaling by Serine/ Threonine Kinase  Receptors

PKC

One critical protein-protein interaction is with PDK-1:PDK-1 phosphorylates PKC auto-phosphorylation of

2 additional residues.Necessary for the newly synthesized PKC isozymes to

achieve catalytically competent conformation.Inactive conformation: binds AKAPs (anchoring kinase associated

proteins) and 14-3-3.Active conformation: RACKs (receptors for activated C kinase)

Both kinds of binding proteins aid in shuttling PKC to specific compartments.

Page 20: Signaling by Serine/ Threonine Kinase  Receptors

PKG

A dimer of 2 identical subunits.Compared to the other kinases, very limited

cellular distribution.The 2nd messenger, cGMP, very limited in its

actions.

Page 21: Signaling by Serine/ Threonine Kinase  Receptors

2nd-Messenger-Independent Protein Kinases

MAPK Cascade.

See next slide for repertoire of neuronal GEFs that convey on Ras and Rap the extranuclear signals leading to MAPK activation and gene expresion.

Page 22: Signaling by Serine/ Threonine Kinase  Receptors
Page 23: Signaling by Serine/ Threonine Kinase  Receptors

Neuronal Substrates of Kinases

Neurotransmitter ReleaseE.g., PKA and CaMKII phosphorylate synapsin I in

the N-terminus.CaMKII phosphorylates synapsin I in the C-terminus.ERKs phosphorylate synapsin I in both N- and C-terminus.

Page 24: Signaling by Serine/ Threonine Kinase  Receptors

Neuronal Substrates of Kinases

Ligand-gated ion channels and K+ channelsE.g., CaMKII phosphorylates AMPARs (LTP).E.g., PKA phosphorylates GluR1 subunits . incr

P(opening) of AMPARs.E.g. PKC phosphorylates GluR2 subunits

differentially regulates its interaction with PDZ domain proteins, GRIP1 and PICK1.

E.g., PKC, PKA, CaMKII, and ERK all phosphorylate Kv4.2 (VG K+ channel).

E.g., PKC and PKA phosphorylate the Kir1 channel.

Page 25: Signaling by Serine/ Threonine Kinase  Receptors

Neuronal Substrates of KinasesTranscription Factors

Many Different Kinases Phosphorylate CREB

CREB – end-point of several signaling pathways

Page 26: Signaling by Serine/ Threonine Kinase  Receptors

Role of the Kinases in Synaptic Transmission and Cross-Talk Between the Different kinase

Pathways

Page 27: Signaling by Serine/ Threonine Kinase  Receptors

Role of Kinases in Synaptic Plasticity

• PKA• CaMKII• PKC• MAPKs

Page 28: Signaling by Serine/ Threonine Kinase  Receptors

Post-synaptic Signaling by Glutamate Release in an Excitatory Synapse

Page 29: Signaling by Serine/ Threonine Kinase  Receptors

Serine Threonine Phosphatases• Critical for signaling and regulating complex

neuronal functions (e.g., synaptic plasticity) activated by phosphorylation.

• Arise from different genes with no homology.• Impossible to i.d. consensus sequences for their

specificity of substrate recognition.• The same phosphatases can dephos substrates

that have been phos by different kinases.• A limited number of multifunctional phosphatases

(PP1 and PP2B) account for most phosphatase activity.

Page 30: Signaling by Serine/ Threonine Kinase  Receptors

Serine Threonine PhosphatasesProtein Phosphatase 2B (PP2B, Calcineurin)• Ca/CaM-dependent phosphatase.• Highly enriched in brain.• Heterodimer [A (60 kDal) and B (19 kDal )

subunits].• A subunit: N-term cat domain and C-term reg

domain.• B subunit: CaCaM binding domain.• PP2B activated by low [Ca2+].

Page 31: Signaling by Serine/ Threonine Kinase  Receptors

Serine Threonine PhosphatasesProtein Phosphatase 2B (PP2B, Calcineurin)• At least 3 PP2B binding proteins have been

identified as inhibitors of the enzyme: CAIN, AKAP 79, and FKBP12.

• E.g., FKBP12 promotes the assoc of PP2B to ryanodine and IP3 receptors allowing PP2B to regulate [Ca2+]free cyto.

• E.g., AKAP 79 binds both PP2B and PKA, bringing them close together for the regulation of receptors and ion channels.

Page 32: Signaling by Serine/ Threonine Kinase  Receptors

Serine Threonine PhosphatasesProtein Phosphatase 2B (PP2B, Calcineurin)• PP2B expressed highly in hipp: in dendritic

spines, but is virtually absent in glia and interneurons.

• PP2B is specific for CREB’s ser133.• In mice over-expressing the auto-inhibitory

domain of PP2B, LTP, at subsaturating tetanization, but not saturating conditions, was enhanced

• Inhibition of PP2B facilitates LTP formation.

Page 33: Signaling by Serine/ Threonine Kinase  Receptors

Serine Threonine PhosphatasesProtein Phosphatase 1 (PP1)• Several subunit catalytic isoforms, α, β, γ1,

and γ2, interacts with other proteins in subcellular targeting.

• E.g., PP1 binds Yotiao, which, in turn, also binds NR1 receptors and the RII reg subunit of PKA so that PP1 and PKA activity are target to the NMDARs.

• PP1 activity can be regulated in various ways, including direct inhibition:

Page 34: Signaling by Serine/ Threonine Kinase  Receptors

Serine Threonine PhosphatasesProtein Phosphatase 1 (PP1)• There are at least 4 known PP1 inhibitory

proteins: Inhibitor-1 (I1), inhibitor-2 (I2), dopamine, and cAMP-regulated phosphoprotein (DARP-32), and nuc inhibitor (NIPP1).

• PP1 activity is differentially regulated by the phosphorylation of proteins:Phos of I1 and DARP-32 inhibits PP1 activity;Phos of I2 and NIPP1 activate PP1.

Page 35: Signaling by Serine/ Threonine Kinase  Receptors

PKA

I1 (T35) and DARP-32 (T34)

ATP

ADP

PP2B

ADP

ATP

Gating Mechanism for PP1 Regulation

cAMP pathway activation would phosphorylate and activate DARP32

Ca2+ pathways activationwould dephosphorylate and inactivate DARP-32

Signals