Select “Start New Project”

Select “Add Files”

2. Select “Add Selected Files”

1.Browse & highlight .fsa files

3. Select “OK”

1.Select “Map Marker 1000” 2.Select “Sizing Default – NPP”

3. Select “Analyze”

4. Select “View”

1. Uncheck all boxes except blue.

2. Select “Edit Plot Settings”

1. Uncheck “Overlay Sizing Curve.”2. Close window.

1.Move arrow under “1000”2.Drag magnifying glass left to “0”

Select “Print”

1. Select “Adobe PDF”2. Select “Preferences”

1.Select “Landscape”2.Select “OK”

Press “ctrl p” or select “Print” in “File” menu.

Select “Microsoft Office Document Image Writer” & “Current view”

Select “OK”

Select “OK”

Select “Microsoft Office Document Image Writer”

Select “Current view”

Press “ctrl a” & “ctrl I” to select & copy image.Press “ctrl v” to paste imageinto Powerpoint file.

Select “Sizing Table View” & “Edit Table Settings”

Highlight 7-10Select “Remove Selected”

1. Highlight 1

2. Select “Remove Selected”

3. Select “Apply”4. Close window

Select “Export”

Open the “.txt” file.Press “ctrl a” & “ctrl c” To select & copy data.

Press “ctrl v” to paste data into Excel file.

Delete: “B”peaks < 21 bases.

Delete: “B”peaks > 950 bases

Delete: “G,” “Y”& “R” peaks

Save Excel & Peak Scanner (“.pjc”) filesin your folder.