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Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 106(8): 957-967, December 2011

Search for a platelet-activating factor receptor in the Trypanosoma cruzi proteome: a potential target for Chagas disease chemotherapy

Daniel Fábio Kawano1/+, Vinicius Barreto da Silva1, Daniel Macedo de Melo Jorge2, Carlos Henrique Tomich de Paula da Silva1, Ivone Carvalho1

1Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto 2Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Av. do Café s/n, 14040-903 Ribeirão Preto, SP, Brasil

Chagas disease (CD) causes the highest burden of parasitic diseases in the Western Hemisphere and is therefore a priority for drug research and development. Platelet-activating factor (PAF) causes the CD parasite Trypanosoma cruzi to differentiate, which suggests that the parasite may express PAF receptors. Here, we explored the T. cruzi proteome for PAF receptor-like proteins. From a total of 23,000 protein sequences, we identified 29 hypothetical proteins that are predicted to have seven transmembrane domains (TMDs), which is the main characteristic of the G protein-coupled receptors (GPCRs), including the PAF receptor. The TMDs of these sequences were independently aligned with domains from 25 animal PAF receptors and the sequences were analysed for conserved residues. The conservation score mean values for the TMDs of the hypothetical proteins ranged from 31.7-44.1%, which suggests that if the putative T. cruzi PAF receptor is among the sequences identified, the TMDs are not highly conserved. These results suggest that T. cruzi contains several GPCR-like proteins and that one of these GPCRs may be a PAF receptor. Future studies may further validate the PAF receptor as a target for CD chemotherapy.

Key words: PAF - GPCR - Trypanosoma cruzi - Chagas disease - bioinformatics - proteome

processes, including inflammation, platelet aggrega-tion and immune response (Harayama et al. 2008). In T. cruzi, PAF proved to trigger cell differentiation to the infective and replicative parasite forms, suggesting the existence of specific molecular recognition sites that can be explored as potential drug targets (Rodrigues et al. 1996, Ambrósio et al. 2003). This hypothesis is sup-ported by the fact that PAF effects in T. cruzi are totally abrogated in the presence of the PAF receptor antago-nist WEB 2086, possibly acting via a seven transmem-brane domain (TMD) receptor, a G protein-coupled receptor (GPCR), as observed in mammals (Rodrigues et al. 1996). Additional studies also demonstrated that PAF was only active on intact parasite cells, suggesting the existence of PAF-mediated intracellular signalling mechanisms (Rodrigues et al. 1999).

Although endogenous PAF has been identified in T. cruzi and immunofluorescence assays indicate that PAF binding sites are present on the parasite membrane, at-tempts to isolate T. cruzi PAF receptors have failed (Gomes et al. 2006). We hypothesised that these PAF receptors proteins are encoded by the T. cruzi genome, which has already been sequenced (Atwood 3rd et al. 2005).

Recent advances in bioinformatics have facilitated the discovery of new drug targets (Chen & Chen 2008, Alves-Ferreira et al. 2009). Here, we evaluated bioinformatic approaches that were previously used to discover new GPCRs in fungi, plants and invertebrates (Kulkarni et al. 2005, Gookin et al. 2008, Kamesh et al. 2008) to identify PAF receptor-like proteins in the T. cruzi proteome.

MATERIALS AND METHODS

The PAF receptor sequences from 25 different ani-mal species were retrieved from Uniprot (uniprot.org/) and ENSEMBL (ensembl.org/) (Supplementary data).

Financial support: FAPESP+ Corresponding author: [email protected] 8 July 2011Accepted 8 November 2011

Chagas disease (CD), or American trypanosomiasis, is a tropical parasitic disease that affects approximately 10 million people in Latin America (WHO 2010). CD is caused by the flagellated protozoan Trypanosoma cruzi, which is often transmitted to humans and other mammals by blood-sucking insects, such as the tri-atomines (Urbina et al. 2003). Transmission can also occur via blood transfusion (Young et al. 2007), organ transplantation (Kun et al. 2009), ingestion of contami-nated foods or fluids (Benchimol Barbosa 2006) or congenital acquisition (Dorn et al. 2007). CD kills ap-proximately 10,000 people every year (WHO 2010) and has a direct impact on workforce productivity in Latin America (Conteh et al. 2010).

Although CD causes the highest burden of parasitic diseases in the Western Hemisphere (Bern & Montgomery 2009), current treatments are highly toxic and often inef-fective, particularly for the chronic stage of the disease (Trouiller et al. 2002). Strikingly, no drug has emerged as an effective candidate for clinical trials in the last 30 years (Moreira et al. 2009). Therefore, the identification of an enzyme or receptor that is essential for parasite growth and/or survival in vivo may facilitate the devel-opment of safer and more effective drugs (Wang 1997).

Platelet-activating factor (PAF) is a potent lipid me-diator that plays a role in several human physiological

Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.958

The T. cruzi proteome was downloaded from TriTrypDB (tritrypdb.org/) (Aslett et al. 2010).

Phylogenetic analyses - The PAF receptor sequenc-es were converted to Molecular Evolutionary Genetics Analysis (MEGA) file format with the MEGA 4.0 soft-ware (Tamura et al. 2007) and then aligned using Multi-ple Sequence Alignment by Log-Expectation (MUSCLE) (Edgar 2004). The phylogenetic analyses were performed with MEGA with the neighbour-joining algorithm (Saitou & Nei 1987). The bootstrap support values for the op-timal trees were calculated using 1,000 replicates with heuristic search settings that were identical to the origi-nal search settings (Felsenstein 1985). The evolutionary distances were calculated with the Jones-Taylor-Thornton matrix-based method (Jones et al. 1992) with respect to the units of the number of amino acid (AA) mutations per site. The indels were treated as missing data.

PAF receptor conserved residue analysis - The resi-dues that are totally conserved among PAF receptors were analysed with MEGA through multiple sequence alignments. The sequences that correspond to the seven membrane-spanning TMDs of the PAF receptors were inferred by alignment to the published human PAF re-ceptor sequence (Gui et al. 2007).

ConSurf-conservation analysis (Ashkenazy et al. 2010) was performed using the tertiary structure of the human PAF receptor as the three-dimensional scaffold (PDB code 2B0X). Multiple sequence alignments of the 25 PAF receptors were computed using MUSCLE (Edgar 2004), and the Bayesian method (Mayrose et al. 2004) was used to calculate the conservation scores with the Jones-Taylor-Thornton matrix-based method (Jones et al. 1992) as a model for protein substitutions.

Search for PAF receptor homologues - The search for PAF receptor homologues was performed with the Basic Local Alignment Search Tool (BLAST)P algo-rithm (Altschul et al. 1990) in the National Center for Biotechnology Information (NCBI) BLAST package version 2.2.24. The search was optimised to detect or-thologues by selecting soft filtering (chosen in the stand-alone NCBI version of BLAST with the -F ‘‘m S’’ op-tion) and the Smith-Waterman algorithm was used to produce the final alignments and calculate the scores (-s T option) (Moreno-Hagelsieb & Latimer 2008). The T. cruzi proteome was used as query in BLASTP against PAF receptor sequences with an e-value threshold of 10 (default). To decrease the possibility of missing a puta-tive PAF receptor sequence, we selected all sequences that were above the threshold value for conservational analyses, not just the reciprocal best hits, which are typi-cally considered for orthologue detection.

T. cruzi hypothetical protein conservation analysis - The T. cruzi sequences that were obtained from the BLAST searches were classified by protein description and the ConPred II software was used to select proteins that have a predicted transmembrane topology. The pro-gram predicts TMDs based on a consensus approach by combining the results of several methods, including KKD, TMpred, TopPred II, DAS, TMAP, MEMSAT 1.8, SOSUI, TMHMM 2.0 and HMMTOP 2.0 (Arai et al.

2004). The theoretical molecular weights of these pro-teins were predicted using the Sequence Manipulation Suite tool (bioinformatics.org/sms2/protein_mw.html).

The sequences of hypothetical proteins that were predicted to contain seven TMDs were manually edited to separate the transmembrane regions, which were then independently aligned with the corresponding TMDs of the 25 PAF receptors using MUSCLE (Edgar 2004). A conservation score for each AA position in the TMD multiple sequence alignment was computed with the SCORECONS web server with default parameters (ebi.ac.uk/thornton-srv/databases/cgi-bin/valdar/scorecons_server.pl) (Valdar 2002). The method generates conser-vation scores for individual residues based on norma-lised substitution matrices or multiple alignments of a set of sequences (Valdar 2002). To reduce the apparent weight assigned to the PAF receptor sequences in the fi-nal score, we highlighted conservation in each AA posi-tion of the hypothetical proteins and rescaled the scores between 0-99, as previously proposed by Haubertin et al. (2006): Rscore = 99 - [99 × (S - Lscore)/Δscore], where Rscore is the rescaled score, S is the score calculated with SCORECONS, Lscore is the lowest score for a giv-en AA position and Δscore is the amplitude of all of the scores for the AA residue. The Rscores that were derived from gapped positions in the T. cruzi hypothetical pro-tein sequences with respect to the scaffold were defined as zero. With the rescaled scores from each AA residue, the weighted means were calculated for each TMD us-ing the following formula, x = ∑(x.w)/∑w , where x is the weighted mean, x the Rscore value for a given AA residue and w is the corresponding conservation weight (score) calculated with SCORECONS when only the multiple sequence alignment of the 25 PAF receptor sequences was used as the input. The final conserva-tion scores were calculated by the weighted mean of the rescaled scores regarding all AA residues in the seven TMDs predicted for the given hypothetical protein.

RESULTS

We assessed the evolutionary relationship of PAF re-ceptors from 25 different animal species (Supplementa-ry data). To the best of our knowledge, these are the only PAF receptor sequences that are currently available. The phylogenetic tree (Fig. 1) clearly indicates that these PAF receptors are split into two main branches. One cluster contains the mammals and the amphibian/bird clades and the second cluster exclusively contains fish. The mammalian lineage can be further subdivided into two branches, where the opossum and platypus are separated from the mammals. The human, orangutan and chim-panzee PAF receptors were sub-grouped together.

The multiple sequence alignment of the PAF recep-tors (Supplementary data) shows that 77 AAs are com-pletely conserved among the 25 species. The majority of these residues (approximately 82%) are located in the TMDs (Fig. 2A, Table I). When residues that are par-tially conserved among species are analysed (shown in pink in Fig. 2B), the alignment clearly shows that these residues are also in the TMDs, except for the fourth do-main, which is the most variable TMD (Table I).

959Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 106(8), December 2011

To identify T. cruzi PAF receptor protein sequences, the 23,031 total T. cruzi protein sequences available in TriTrypDB (Aslett et al. 2010) were compared to other the 25 PAF receptor sequences via a BLAST search with an E-value threshold of 10. A total of 264 parasite pro-tein sequences were selected, of which 138 were identi-fied as potential PAF receptor proteins. A comparison of these hypothetical proteins with the PAF receptors yields similar BLAST best score values that range from 17-29. The hypothetical protein Tc00.1047053511507.60 presented the highest number of BLAST hits: 24 from the 25 PAF receptor sequences (Table II).

Of the 138 hypothetical T. cruzi proteins identified, 29 sequences were predicted to contain seven TMDs with chain lengths that ranged from 305-1,410 AA. TMD se-quence of these hypothetical proteins was analysed for AA conservation and the resulting mean values for the seven TMDs ranged from 31.7-44.1%. When the TMDs were analysed independently, no T. cruzi hypothetical protein exhibited a weighted mean value above 60% (Table III).

DISCUSSION

Several groups have previously proposed that PAF exerts a biological effect on T. cruzi by binding to trans-membrane receptors (Rodrigues et al. 1996, Ambrósio et

al. 2003, Gomes et al. 2006). PAF receptors are GPCRs, which are transmembrane signalling proteins that are evolutionarily conserved among eukaryotic organisms (Römpler et al. 2007). The occurrence of GPCRs and G-protein signalling dates back 1.2 billion years, which was

Fig. 1: evolutionary relationships of platelet-activating factor (PAF) re-ceptors from 25 animal species. The evolutionary relationship of PAF receptors was inferred using the neighbour-joining method (Saitou & Nei 1987). The bootstrap consensus tree inferred from 1,000 repli-cates (Felsenstein 1985) is taken to represent the evolutionary history of the taxa analyzed. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to in-fer the phylogenetic tree. The evolutionary distances were computed using the Jones-Taylor-Thornton matrix-based method (Jones et al. 1992) and are in the units of the number of amino acid (AA) substitu-tions per site. The analysis involved 25 AA sequences. All positions containing gaps and missing data were eliminated. There were a to-tal of 281 positions in the final dataset. Evolutionary analyses were conducted in Molecular Evolutionary Genetics Analysis 4.0 software (Tamura et al. 2007).

Fig. 2A: conservation pattern of platelet-activating factor (PAF) re-ceptors from 25 animal species. Residues totally conserved among re-ceptors are highlighted in yellow. Multiple sequence alignments were performed using Multiple Sequence Alignment by Log-Expectation (MUSCLE) (Edgar 2004) and the analysis of conserved residues was carried out in Molecular Evolutionary Genetics Analysis (MEGA) 4.0 software (Tamura et al. 2007). The two-dimensional representa-tion of human PAF receptor was adapted from Gui et al. (2007); B: conservation profile for the tertiary structure of PAF receptor (PDB code 2B0X) using ConSurf-conservational analysis. Protein was vi-sualized using FirstGlance in Jmol with colour-coded conservation scores. The conserved and variable residues are coloured according to the conservation scores. Sites, for which the inferred conservation level was assigned with low confidence, are coloured light yellow. Multiple sequence alignments of the 25 PAF receptors were computed using MUSCLE and the Bayesian method (Mayrose et al. 2004) was applied for the calculations of the conservation scores using the Jones-Taylor-Thornton matrix-based method (Jones et al. 1992) as the model of substitution for proteins.


before plants, fungi and animals emerged from a common ancestor (Schöneberg et al. 2007). Thus, it seems reason-able that T. cruzi may also express a PAF receptor.

Current theory regarding receptor structure and function evolution suggests that the structural diversity of the receptor between different species is a result of evolution, which is characterised by the continuous ac-cumulation of mutations. However, if receptor function is essential for survival of the organism, some struc-tural conservation is required to ensure protein function (Schöneberg et al. 2007).

Our studies regarding PAF receptor evolution indi-cate that the TMDs have the highest density of conserved residues. This observation is consistent with molecular modelling studies, which suggest that the ligand-binding site of the human PAF receptor is fundamentally com-posed of TMD residues (Gui et al. 2007). Moreover, the TMDs tend to be conserved among GPCRs, while the extra and intracellular loop regions and C-terminus are typically more divergent (Herz & Thomsen 2007).

Based on similarity to known PAF receptor protein sequences, we identified 29 hypothetical seven TMD proteins in the T. cruzi proteome. To the best of our knowledge, these proteins represent the first putative GPCRs identified in this parasite. Although the evo-lutionary origins of the encoding genes remain un-known, previous studies have indicated that horizontal gene transfer (the process where genetic material is transferred between distinct evolutionary lineages) has occurred in parasitic protozoa (Richards et al. 2003); moreover, viral infection may explain the origin of sev-eral mammalian genes, including genes that code for membrane receptors (Yu et al. 1996).

An alternative hypothesis is that putative GPCRs have been present since the beginning of trypanosome

evolution. For example, the discovery of specific GP-CRs in the protozoan Dictyostelium discoideum that were previously thought to be only in animals suggests that these genes arose before the divergence of animals and fungi and were subsequently lost in fungi (Prabhu & Eichinger 2006).

Our evolutionary analysis of previously identified PAF receptors indicates that the fish receptor sequences are grouped into a distinct clade from the other PAF re-ceptors. Danio rerio, Gasterosteus aculeatus, Oryzias latipes and Tetraodon nigroviridis are teleost fishes, some of the most primitive vertebrates (Verburg-van Ke-menade et al. 2009), thus pointing to the use of a PAF re-ceptor sequence from a fish (instead of from a human) as the reference to calculate the percentages of un-gapped AA residues in the conservation analyses of hypotheti-cal proteins. However, in view of the considerable evolu-tionary distance between GPCRs from animals and their putative counterparts in T. cruzi (Wilkie & Kinch 2005), we believe that the advantage of using a PAF receptor sequence from a fish as the reference could be assumed as insignificant. Besides, the fact that both PAF and its antagonist WEB 2086 are active in human and T. cruzi (Rodrigues et al. 1996) suggests some conservation be-tween these species, but the same cannot be stated for fishes and T. cruzi.

A comparison of the identified T. cruzi seven TMD proteins and the human PAF receptor sequence resulted in relatively similar low BLAST scores. However, the BLAST algorithm typically does not provide high scores among phylogenetically distant species because genom-es with a large evolutionary distance typically have a low protein sequence similarity (Kim et al. 2008). Thus, it would be optimal to use PAF receptor sequences from more primitive organisms, such as Dictyostelium or En-tamoeba, which have genes that encode GPCRs (Wilkie & Kinch 2005). However, because no PAF receptor se-quence from a lower eukaryote is currently available, a more precise relationship cannot be established.

Conservation analyses of the TMD AAs of the hy-pothetical T. cruzi proteins did not allow us to identify a protein sequence that is orthologous to the human PAF receptor. Although the weighted mean conserva-tion scores that we calculated cannot be assumed to be absolute percentages of AA conservation relative to the PAF receptors, we expect that a putative PAF receptor orthologue would exhibit a higher number of conserved motifs; consequently, this should be expressed as a con-siderably higher conservation score compared to the re-maining non-orthologous proteins. Nevertheless, if the putative T. cruzi PAF receptor is among the sequences we identified, this receptor does not show a high degree of conservation in the TMDs compared to animal PAF receptors. This is apparent in the protozoan D. discoi-deum, where the seven TMD receptors show little se-quence similarity to the corresponding GPCRs in ani-mals (Lagerström & Schiöth 2008).

The 29 putative seven TMD proteins identified in this study represent a starting point for additional bio-chemical, physiological and molecular biology studies

TABLE IConserved residues in the transmembrane domains of

platelet-activating factor (PAF) receptors from 25 animal species analyses of residues that are totally conserved among

PAF receptors were carried out in Molecular Evolutionary Genetics Analysis (Tamura et al. 2007) and residues partially

conserved were computed using ConSurf-conservation analysis (Ashkenazy et al. 2010)

Domain

Residues totallyconserved

(n)

Residues partiallyconserve

(n)

Total of residues

(n)

I 10 18 30II 12 23 30III 13 21 33IV 4 7 23V 3 11 26VI 10 24 31VII 11 24 31


TABLE IIHypothetical proteins of Trypanosoma cruzi detected via Basic Local Alignment Search Tool (BLAST)

search against platelet-activating factor (PAF) receptors from 25 animal species prediction of transmembrane domains (TMDs) was carried out with ConPred II software (Arai et al. 2004)

Hypothetical protein

BLAST

Length(AA)

Weight(kDa) TMDsHits

Bestscore e-value PAF

Tc00.1047053511573.49 3 29 0.002 Rat 235 27.26 6Tc00.1047053509027.50 19 27 0.041 Rat 534 59.23 6Tc00.1047053503487.70 17 26 0.031 Elephant 382 41.66 8Tc00.1047053511127.300 2 26 0.071 Squirrel 534 59.42 6Tc00.1047053510493.10 5 25 0.057 Mouse 240 25.82 6Tc00.1047053508801.10 12 25 0.10 Opossum 372 41.71 8Tc00.1047053508641.200 5 24 0.14 Elephant 305 32.55 7Tc00.1047053506869.20 3 24 0.20 Platypus 458 52.67 8Tc00.1047053510519.100 3 24 0.20 Platypus 458 52.64 8Tc00.1047053508815.80 3 24 0.23 Chicken 570 64.11 9Tc00.1047053510311.20 13 24 0.30 Frog 801 91.08 6Tc00.1047053508873.530 10 24 0.35 Platypus 1,071 115.86 6Tc00.1047053511629.40 4 23 0.12 Mouse 240 25.84 6Tc00.1047053511285.80 16 23 0.18 Pika 247 27.23 6Tc00.1047053509649.40 16 23 0.19 Pika 247 27.25 6Tc00.1047053508547.140 1 23 0.26 Zebrafish 482 53.43 8Tc00.1047053507943.70 3 23 0.27 Medaka 405 43.59 9Tc00.1047053506681.24 8 23 0.35 Horse 341 38.37 8Tc00.1047053508215.6 4 23 0.40 Platypus 494 55.71 7Tc00.1047053506925.450 3 23 0.40 Opossum 681 77.39 6Tc00.1047053503463.20 4 23 0.41 Platypus 494 55.69 7Tc00.1047053510725.70 4 23 0.46 Opossum 486 54.09 6Tc00.1047053509099.130 16 23 0.50 Human 822 91.69 8Tc00.1047053510885.60 19 23 0.72 Guinea pig 1,151 131.20 6Tc00.1047053508159.26 3 23 1.1 Guinea pig 1,278 137.16 4Tc00.1047053511183.315 2 23 1.1 Medaka 1,085 115.00 4Tc00.1047053510305.79 10 23 1.2 Medaka 1,410 156.58 7Tc00.1047053507941.110 3 22 0.44 Medaka 385 41.43 9Tc00.1047053506825.24 7 22 0.48 Horse 341 38.44 7Tc00.1047053510729.290 20 22 0.62 Platypus 335 37.98 7Tc00.1047053506467.4 3 22 0.67 Stickleback 340 38.27 7Tc00.1047053504137.150 8 22 0.68 Stickleback 340 38.52 7Tc00.1047053505193.74 4 22 0.69 Stickleback 340 38.21 7Tc00.1047053509075.31 3 22 0.70 Mouse 540 58.70 5Tc00.1047053509561.70 11 22 0.72 Pika 306 34.20 6Tc00.1047053510103.30 17 22 0.81 Opossum 594 65.97 5Tc00.1047053506009.60 4 22 0.82 Guinea pig 416 45.27 6Tc00.1047053506179.30 1 22 0.97 Chicken 600 66.99 8Tc00.1047053507663.80 1 22 0.99 Chicken 600 66.77 8



BLAST

Length(AA)



Tc00.1047053509719.56 2 22 1.2 Zebrafish 724 82.56 7Tc00.1047053511461.40 5 22 1.3 Pig 552 62.88 6Tc00.1047053508535.30 4 22 1.3 Pig 553 63.02 7Tc00.1047053508443.50 3 22 1.5 Pufferfish 630 70.30 5Tc00.1047053509157.110 3 22 1.5 Pufferfish 630 70.46 4Tc00.1047053507821.101 2 22 1.5 Rat 994 106.93 4Tc00.1047053507209.60 16 22 1.6 Guinea pig 819 91.35 8Tc00.1047053508153.920 3 22 1.6 Platypus 611 68.86 1Tc00.1047053506529.150 1 22 1.8 Zebrafish 1,151 131.49 6Tc00.1047053511921.71 9 22 1.9 Opossum 1,157 124.18 7Tc00.1047053509849.30 4 22 2.2 Shrew 860 97.79 7Tc00.1047053511185.150 1 22 3.2 Guinea pig 2,247 243.13 -Tc00.1047053508891.80 1 22 4.5 Mouse 1,935 216.30 6Tc00.1047053507615.60 2 21 0.76 Frog 284 32.16 6Tc00.1047053508277.210 21 21 0.81 Stickleback 319 36.83 6Tc00.1047053511495.20 4 21 0.81 Elephant 324 36.45 6Tc00.1047053508489.20 4 21 0.82 Elephant 324 36.39 6Tc00.1047053508799.290 4 21 0.83 Pufferfish 221 24.45 6Tc00.1047053506753.30 19 21 0.87 Elephant 341 37.88 7Tc00.1047053511305.40 4 21 0.88 Chicken 301 32.92 5Tc00.1047053509945.24 22 21 0.89 Stickleback 319 36.79 6Tc00.1047053507257.180 2 21 0.94 Medaka 327 35.92 5Tc00.1047053510355.190 20 21 0.97 Elephant 341 37.92 7Tc00.1047053511507.60 24 21 1.1 Shrew 311 35.11 7Tc00.1047053507883.50 20 21 1.1 Pika 319 35.46 5Tc00.1047053503897.120 11 21 1.1 Pika 306 34.20 5Tc00.1047053510001.31 4 21 1.1 Medaka 317 34.99 6Tc00.1047053503647.40 1 21 1.1 Chicken 409 45.33 9Tc00.1047053508741.20 2 21 1.2 Medaka 319 34.70 9Tc00.1047053506195.50 15 21 1.3 Pika 319 35.29 5Tc00.1047053508041.20 12 21 1.4 Goat 528 60.25 6Tc00.1047053509997.73 3 21 1.4 Platypus 351 38.97 8Tc00.1047053506297.340 12 21 1.5 Goat 528 60.22 6Tc00.1047053507603.220 3 21 1.5 Chicken 444 49.64 6Tc00.1047053506661.140 3 21 1.5 Pika 486 54.79 6Tc00.1047053509269.19 7 21 1.7 Pufferfish 471 53.80 8Tc00.1047053509429.280 5 21 1.9 Chicken 552 62.15 8Tc00.1047053504021.90 1 21 2.3 Guinea pig 600 65.97 9Tc00.1047053507083.100 3 21 2.5 Chicken 662 72.90 8Tc00.1047053507677.60 1 21 2.5 Guinea pig 600 65.87 9Tc00.1047053508317.70 4 21 2.6 Shrew 856 97.38 7Tc00.1047053503735.30 1 21 2.6 Horse 844 95.54 7Tc00.1047053509901.100 3 21 2.9 Goat 662 72.94 8



BLAST

Length(AA)



Tc00.1047053508121.15 2 21 3.1 Chicken 1,027 111.03 4Tc00.1047053510507.60 2 21 4.0 Cow 1,130 127.09 5Tc00.1047053503919.60 1 21 6.1 Squirrel 1,579 177.58 5Tc00.1047053507723.70 1 21 9.1 Rabbit 2,960 326.30 -Tc00.1047053509799.90 4 20 1.4 Frog 310 35.33 7Tc00.1047053509431.10 5 20 1.5 Cow 297 33.22 5Tc00.1047053508739.30 5 20 1.6 Pika 331 36.04 8Tc00.1047053503909.90 23 20 1.7 Mouse 311 35.14 6Tc00.1047053508241.104 2 20 1.7 Stickleback 295 33.58 6Tc00.1047053511693.130 6 20 1.8 Squirrel 383 43.59 8Tc00.1047053506565.29 2 20 2.4 Chicken 425 49.18 9Tc00.1047053509245.29 1 20 2.4 Opossum 315 35.83 8Tc00.1047053507969.30 6 20 2.5 Opossum 470 53.77 7Tc00.1047053504029.90 4 20 2.7 Guinea pig 351 38.96 8Tc00.1047053509799.100 7 20 2.8 Stickleback 340 38.66 7Tc00.1047053511245.24 3 20 2.8 Pika 486 54.56 6Tc00.1047053510311.80 4 20 3.3 Platypus 655 72.93 8Tc00.1047053511801.4 1 20 3.7 Goat 634 70.95 6Tc00.1047053511001.50 3 20 4.3 Shrew 494 54.09 7Tc00.1047053503593.60 1 20 4.4 Medaka 654 72.40 5Tc00.1047053506127.120 1 20 4.5 Medaka 654 72.17 5Tc00.1047053511279.20 2 20 4.7 Pika 709 79.47 6Tc00.1047053508723.24 1 20 4.9 Frog 559 63.22 7Tc00.1047053509791.60 1 20 4.9 Frog 559 63.25 7Tc00.1047053511923.71 10 20 5.0 Mouse 1,103 120.80 7Tc00.1047053509897.132 9 20 5.4 Pika 1,129 119.93 5Tc00.1047053508741.260 1 20 5.5 Elephant 814 90.46 8Tc00.1047053511003.160 2 20 6.2 Rat 1,034 114.04 6Tc00.1047053504033.140 1 20 6.4 Chicken 1,013 113.12 6Tc00.1047053511787.30 1 20 6.9 Rat 1,267 134.46 7Tc00.1047053510861.94 1 20 8.0 Horse 845 95.95 7Tc00.1047053509899.70 1 20 9.0 Pufferfish 1,271 135.75 6Tc00.1047053508347.140 1 19 2.8 Frog 284 32.08 6Tc00.1047053504765.20 17 19 2.9 Pig 269 30.04 6Tc00.1047053504137.140 4 19 3.6 Frog 310 35.44 7Tc00.1047053505757.10 10 19 3.7 Rat 259 28.18 5Tc00.1047053506669.10 10 19 3.7 Rat 259 28.18 5Tc00.1047053510885.20 3 19 3.9 Horse 296 32.15 6Tc00.1047053506529.110 2 19 3.9 Horse 296 32.08 6Tc00.1047053511837.40 1 19 4.1 Medaka 290 32.68 8Tc00.1047053511531.44 2 19 4.7 Platypus 405 44.45 5Tc00.1047053509741.20 1 19 5.2 Stickleback 356 39.50 4Tc00.1047053507681.50 1 19 5.3 Platypus 516 57.64 7



BLAST

Length(AA)



Tc00.1047053507509.80 2 19 5.7 Cow 573 63.50 6Tc00.1047053506871.130 2 19 5.9 Cow 573 63.64 6Tc00.1047053507395.10 8 19 6.4 Elephant 405 45.36 6Tc00.1047053510087.30 7 19 6.4 Elephant 403 45.13 5Tc00.1047053510265.14 1 19 7.5 Rabbit 655 72.97 8Tc00.1047053511471.10 1 19 8.7 Rabbit 796 86.81 1Tc00.1047053430895.10 11 18 4.0 Rat 259 28.24 5Tc00.1047053509253.30 1 18 5.1 Stickleback 265 30.50 5Tc00.1047053508207.120 2 18 6.0 Rat 224 25.28 5Tc00.1047053511683.20 2 18 6.1 Medaka 318 34.60 9Tc00.1047053507625.200 1 18 6.1 Stickleback 355 39.56 9Tc00.1047053402857.10 1 18 7.9 Stickleback 321 34.93 8Tc00.1047053506513.14 1 17 9.0 Guinea pig 222 25.39 6

AA: amino acid.

TABLE IIIConservation analysis of amino acid (AA) residues in the transmembrane domains (TMDs)

of Trypanosoma cruzi hypothetical proteins performed in SCORECONS web server (Valdar 2002) conservation scores for each TMD are expressed as the weighted mean of rescaled scores for each AA position. Percentages of un-gapped

AA residues in the seven TMDs were calculated in comparison to the sequence of human PAF receptor in the multiple sequence alignment containing each hypothetical protein and the sequence of 25 PAF receptors.

Hypothetical proteinLength (AA)

Un-gapped TMDs

residues (%)

Conservation scores (%)

Mean(7 TMDs)

Domains

I II III IV V VI VII

Tc00.1047053508215.6 494 89.1 44.1 55.5 49.3 43.5 44.5 35.3 33.1 45.8Tc00.1047053508723.24 559 83.7 42.9 39.1 38.3 52.4 50.7 44.3 38 38.7

Tc00.1047053509791.60 559 83.7 42.8 39.1 38.2 52.4 50.7 44.3 38 38.7

Tc00.1047053506753.30 341 87.8 42.8 51.1 36.4 41.9 46,3 42.7 28.4 52.5

Tc00.1047053505193.74 340 91.2 42.5 52.1 38.3 45.5 31.3 34.9 44.9 48.2

Tc00.1047053503463.20 494 87.1 42.5 44.7 48.6 43.5 44.5 36.3 33.1 45.8

Tc00.1047053504137.150 340 89.8 42.2 44.3 30.1 42.4 51.3 52.5 47.8 31

Tc00.1047053506467.4 340 91.2 42.2 52.1 38.3 45.5 30.2 34.9 44.9 46.9

Tc00.1047053509799.100 340 89.8 42.1 44.3 30.1 42.4 51.3 52.5 46.7 31

Tc00.1047053508641.200 305 87.8 41.7 48.3 37.1 40.6 41.6 46.5 50.3 28.9

Tc00.1047053511787.30 1,267 89.1 41 29 38.6 36.6 37 51.1 43.7 51.2

Tc00.1047053510305.79 1,410 88.4 40.9 44.1 34.2 31.8 41.6 40 45.5 49.1

Tc00.1047053510861.94 845 95.2 40.8 39.4 35.9 34.7 47.5 42.4 41.4 45.5

Tc00.1047053511923.71 1,103 81.6 40.6 39.9 39.5 40.1 40.8 44.4 25.4 53.3

Tc00.1047053511921.71 1,157 89.8 40.6 39.9 44.9 38.6 30.4 50.5 24.4 53.3Tc00.1047053503735.30 844 92.5 40.6 39.4 38.4 33 47.5 42.4 41.4 43.4


to identify surface proteins in T. cruzi. None of these 29 sequences or any other sequence derived from hy-pothetical proteins in the parasite proteome display a high degree of similarity with the human PAF receptor. However, the identification of the T. cruzi receptor may theoretically enable the design of PAF antagonists that interact with the T. cruzi, but not human PAF receptor. Molecules that affect the growth/differentiation of para-sites can subsequently be tested as selective and effec-tive drugs for the treatment of neglected diseases (Ham-marton et al. 2003).

The limitations of this study include the ability of the BLAST algorithm to correctly identify the putative PAF receptor sequence and the ability to correctly pre-dict TMD structures. To minimise these limitations, we selected a BLASTP algorithm that has been optimised to detect orthologues (Moreno-Hagelsieb & Latimer 2008) and used a consensus approach to properly detect the TMDs (Arai et al. 2004).

Additional limitations of this work may be related to the nature of the proteomic data analysed. In contrast to the kinetoplastid pathogens Leishmania major and Try-panosoma brucei, the T. cruzi assembled genome se-quences are considered to be highly repetitive because the CL Brener reference strain is a hybrid of the “non-Esmeraldo-like” and “Esmeraldo-like” lineages; conse-quently, each chromosome is comprised of two homolo-gous chromosomes (Alves-Ferreira et al. 2009, Weatherly et al. 2009). Therefore, the predicted T. cruzi proteome has some inherent restrictions regarding computational gene prediction and annotation that may hamper the iden-tification of a potential unpredicted and/or un-annotated PAF receptor. Additional studies regarding the identifi-

Hypothetical proteinLength (AA)

Un-gapped TMDs

residues (%)

Conservation scores (%)

Mean(7 TMDs)

Domains

I II III IV V VI VII

Tc00.1047053510355.190 341 87.8 40.5 51.1 36.4 25.9 46.3 41.1 32.2 50.8Tc00.1047053509719.56 724 86.4 40.2 34.7 36.7 46 43.5 42.2 30.9 47.2

Tc00.1047053508317.70 856 86.4 40.1 31.4 38.6 57.3 43 40.4 40.2 30.3

Tc00.1047053509849.30 860 86.4 38.6 47.1 31 38.4 44.6 40.4 40.2 30.3

Tc00.1047053510729.290 335 90.5 38.5 48.7 31.7 43.2 41.7 33.8 36.8 33.7

Tc00.1047053504137.140 310 90.5 38.2 41.9 28.3 44.1 31 36.5 32.8 50.7

Tc00.1047053509799.90 310 93.2 37.7 47.9 28.3 44.1 32.5 33 32.8 44.1

Tc00.1047053507969.30 470 93.2 37.7 36.5 27.2 59.4 39.6 31.8 34.3 35.3

Tc00.1047053511001.50 494 86.4 37.7 33.2 34.6 26.4 41.2 34.9 43.4 50.3

Tc00.1047053507681.50 516 89.8 36.7 48.1 26.2 34.3 34.9 32 41.8 39.4

Tc00.1047053506825.24 341 87.1 34.7 40.1 28.1 25.9 38 42.6 31.3 38

Tc00.1047053511507.60 311 85.7 34.5 31.7 27.4 33.4 36.4 43.8 35.1 35.2Tc00.1047053508535.30 553 87.1 31.7 39.5 19.5 10.7 42.4 43.4 31.7 38

cation of PAF receptor homologues in the complete T. cruzi genome (not only against the predicted proteome) are needed, as they may overcome these limitations.

ACKNOWLEDGEMENTS

To Prof. Marcelo Dias Baruffi, for the continuous support throughout the course of this project.

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1Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.

Sequences platelet-activating factor (PAF) receptors

PAF Sequence ID Source

Bos taurus (cow) Q9TTY5 UniprotCapra hircus (goat) Q9GK76 UniprotCavia porcellus (guinea pig) P21556 UniprotGallus gallus (chicken) E1C095 UniprotHomo sapiens (human) P25105 UniprotMacaca mulatta (rhesus monkey) P35366 UniprotMus musculus (mouse) Q62035 UniprotRattus norvegicus (rat) P46002 UniprotSus scrofa (pig) Q9XSD4 UniprotXenopus laevis (African clawed frog) Q7ZWY0 UniprotDanio rerio (zebrafish) ENSDARP00000094880 Ensembl Equus caballus (horse) ENSECAP00000003792 Ensembl Erinaceus europaeus (hedgehog) ENSEEUP00000012269 Ensembl Gasterosteus aculeatus (three-spined stickleback) ENSGACP00000012761 Ensembl Loxodonta africana (elephant) ENSLAFP00000006539 Ensembl Monodelphis domestica (opossum) ENSMODP00000032054 Ensembl Ochotona princeps (American pika) ENSOPRP00000002162 Ensembl Ornithorhynchus anatinus (platypus) ENSOANP00000021295 Ensembl Oryctolagus cuniculus (rabbit) ENSOCUP00000015312 Ensembl Oryzias latipes (medaka) ENSORLP00000015743 Ensembl Pan troglodytes (chimpanzee) ENSPTRP00000000744 Ensembl Pongo pygmaeus (orangutan) ENSPPYP00000001903 Ensembl Sorex araneus (shrew) ENSSARP00000005182 Ensembl Spermophilus tridecemlineatus (squirrel) ENSSTOP00000014258 Ensembl Tetraodon nigroviridis (green pufferfish) ENSTNIP00000020395 Ensembl


ConSsurf colour-coded multiple sequence alignment of platelet-activating factor receptors


Mul

tiple

sequ

ence

alig

nmen

ts o

f tra

nsm

embr

ane

dom

ains

from

Try

pano

som

a cr

uzi h

ypot

hetic

al p

rote

ins a

nd p

late

let-a

ctiv

atin

g fa

ctor

rece

ptor

sSe

quen

ceD

omai

n I

Dom

ain

IID

omai

n II

ID

omai

n IV

Dom

ain

VD

omai

n V

ID

omai

n V

IITc

00.1

0470

5350

8215

.6--S

RVW

FVAF

TFGM

TGHS

SVM

TM-

-PIM

ISCT

LLPL

VFLG

FLLR

I---

----C

LGFL

SDAF

VLM

FGIT

ALAS

Q---

FLGF

SILI

SCTR

LVSI

LILF

PVA

VFPI

MFG

YAFC

GHVV

FGAF

------

--SRS

IVTL

FCTT

FGDN

IIDTF

L-F

GRLF

IGTY

LLL-

-FIC

NIL

NVA

Hum

an-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAF-

LGVI

-----I

SLSL

VIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Chic

ken

--TVF

YSIIF

ILGF

VAN

CYVL

WI-

--FM

LNLT

VADL

LFLV

TMPL

WIV

-SV

AGSL

FFVN

TYAS

VAF-

LMVI

----G

IFLS

AAIW

IITVG

SALY

YIF-

-AI

HIIIC

TLFY

VIFL

FILT

WN

---RA

LWM

VCTV

LVVF

IISFV

PHH-

----

-VTL

CLLS

MN

CVLD

PVIY

CFLT

--Ch

impa

nzee

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

F-LG

VI---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

--Co

w-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

FFFI

NTY

CSVA

F-LA

VI---

--ILL

SLIIW

VSIV

GAAS

YFFV

L--IH

IFLV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPI

IYCF

LT--

Elep

hant

-FPI

FYSI

IFVL

GVIA

NGY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCLF

FIN

TYCS

VAF-

LGVI

-----I

TLSL

VIW

VTIV

AAAS

YFLF

M-

--HIF

IVCS

FFLV

FFIIL

VCN

LV-

RALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Frog

---VT

YSIIF

VFGF

IAN

CYVL

WIF

-IFM

INLT

IADL

LFLV

TLPL

WI--

NVA

GCFF

FLN

TYCS

VAF-

LGVI

------

CISV

AVW

ILLF

ASSL

YFLI

FP--H

FILV

GAFF

VVFL

IILTC

NLV

-RA

LWM

VVTV

LAVF

VLCF

VPHH

-----

---LC

LMST

NCM

LDPI

IYCF

LTKK

Goat

-FPI

FYSI

VFVL

GVIA

NSY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCFF

FIN

TYCS

VAF-

LAVI

-----F

LLSL

IIWVS

IVGA

ASYF

FVL-

-IHIF

LVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFVI

CFVP

HH---

---V

TLCL

LSTN

CVLD

PIIY

CFLT

--Gu

inea

pig

-FPI

VYSI

IFVL

GIIA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAF-

LGVI

-----I

ALSL

VIW

VAIV

AAAS

YFLV

M-

-IHIC

IVLG

FFIV

FLLI

LFCN

L--

RALW

MVC

TVLA

VFVI

CFVP

HH---

--Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Hedg

ehog

-FPI

FYSI

IFAL

GVIA

NSY

VLW

----F

MVN

LTLA

DLLF

LVTL

PLW

II-N

LAGC

FFFI

NTY

CSVA

F-LG

VI---

-GIIL

SLVI

WVA

IVSA

ASYF

FF--

-IHIF

LVVS

FFVV

FLIIF

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

ITLC

LLST

NCV

LDPI

IYCF

L---

Hors

e--P

IVYS

IIFVL

GVIA

NSY

VLW

V--IF

MVN

LTVA

DLLF

LLTL

PLW

V--

NLT

GCCF

FIN

TYCS

VAF-

LGVI

-----I

SLSL

VIW

LAIV

AAAS

YFLF

M-

-IHIF

LVFS

FFLV

FLIIL

VCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPI

IYCF

L---

Med

aka

-FSV

VYSI

FFVV

GLLA

NVY

VLF-

---Y

MTN

LTIA

DLLF

VCAL

PFW

IG-

--TGS

FFFI

NTY

GTIL

F-LG

AISL

-RG

IIVSV

LIW

LCLV

SSAI

PSL-

----H

FLIIG

LFFV

VFFL

VMVC

NLL

----

QM

LLAV

VGVF

LLCF

LPHH

VVQ

--Q

ITLV

LMGL

NCI

LDPV

IYYF

A---

Mon

key

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

F-LG

VI---

--ISL

SLVI

WVA

IVGA

ASYF

FIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Mou

se--F

MVN

LTM

ADLL

FLIT

LPLW

IV-

-FPI

VYSV

IFIL

GVVA

NGY

VLW

--N

VAGC

LFFI

NTY

CSVA

F-LG

VI---

-GIS

LSLI

IWVS

IVAT

ASYF

LA--

VVHV

FIAF

CFFL

VFFL

IFYC

N---

RALW

MVC

TVLA

VFIIC

FVPH

H----

--IT

LCLL

STN

CVLD

PVIY

CFLT

--O

poss

um-F

PIVY

SIIF

VLGF

VSN

AYVL

W--

---M

VNLT

VADL

LFLF

ALPL

WIV

YN

VAGC

LFFI

NTY

CSVC

F-LG

VI---

--HVF

IVLC

FFIV

FLVI

LVCN

VI-

-GIL

LSTI

IWVV

IVGC

ASYF

LI--

--LW

MVC

TVM

AVFF

ICFV

PHHV

V---

QVT

LCLM

SIN

CVLD

PIIY

CFL-

--O

rang

utan

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

F-LG

VI---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

--Pi

g-F

PIFY

SIIF

VLGV

IAN

SYVL

W--

NLA

GCFF

FIN

TYCS

VSF-

LAVI

------

MLN

LTM

ADLL

FLVT

LPLW

IIY--I

SLSL

IIWVA

MVA

AASY

FFVL

--IH

IFLV

FSFF

LVFL

VILF

CNL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-ITLC

LLST

NCV

LDPI

IYCF

LT--

Pika

-FPI

VYSI

IFVL

GVVA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

II-N

VAGC

LFFI

NTY

CSVA

F-LG

VI---

--ISL

SLIIW

VVIL

AIAS

YFLI

M-

-IHIF

IVFC

FFVV

FLIIL

VCN

L--

QVT

LCLL

STN

CVLD

PVIY

CFL-

--RA

LWM

VCTV

LGVF

IICFV

PHH-

----

Plat

ypus

---VA

YSIIF

VLGI

FAN

GYVL

WVF

--FM

VNLT

VADL

LFLV

TMPL

WIV

-N

VAGC

LFFI

NTY

CSVA

F-LG

VI---

-GIV

LSLV

IWVL

IVSS

ALYF

LV--

-IHIF

IVAS

FFAV

FLLI

LVCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

--Pu

fferfish

--PSA

YGVI

FILG

LLAN

LYVL

FI-

---M

TNLS

IADL

LFVC

ALPF

WIG

Y---

GSLF

FIN

TYCS

VLF-

LGAI

SVN

-GIII

SVIIW

VLTI

SMAI

PYLV

-----

FAIIG

LFFV

VFAL

VLVC

NLL

I---

--LLA

VVGV

FVVC

FLPH

HVIQ

GAQ

ITLL

LMGL

NCI

LDPV

VYCF

A---

Rabb

it--P

IVYS

IIFVL

GVVA

NGY

VLW

V---F

MVN

LTVA

DLLF

LITL

PLW

II-N

LAGC

LFFI

NTY

CSVA

F-LG

VI---

--IAL

SLVI

WVS

ISAA

ATYF

LIL-

-IHIF

IVAS

FFIV

FLIIL

ICN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Rat

--PIV

YSVI

FVLG

VVAN

GYVL

WV-

--FM

VNLT

VADL

LFLM

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

F-LG

VI---

-GIT

LSLV

IWIS

IAAT

ASYF

LA--

-VHI

FITS

CFFL

VFFL

IFYC

NM

--RA

LWM

VCTV

LAVF

VICF

VPHH

-----

-ITLC

LLST

NCV

LDPV

IYCF

LT--

Shre

w-F

PIFY

SIIF

VLGV

IAN

SYVL

W--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

-N

LTGC

FFFI

NTY

CSVA

F-LG

VI---

-GIY

LSLV

IWM

AIVS

AASY

FFF-

--IH

IFLV

CSFF

LVFL

LIFF

CNL-

-RA

LWM

VCTV

LAVF

SICF

VPHH

-----

-VTL

CLLS

TNCV

LDPI

IYCF

LT--

Squi

rrel

LFPI

IYSI

IFVV

GVIA

NGY

VL---

--FM

LNLT

VADL

LFLI

TLPL

WII-

NVA

GCLF

FIN

TYCS

VAF-

LGVI

------

YLSL

VIW

VFIV

AAAS

YFLI

LDVI

HIFI

VFSF

FLVF

LIIL

FCK-

--RA

LWM

VCAV

LAVF

VICF

VPHH

-----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--Sti

ckleba

ck-F

PVVY

SIIF

ILGI

TAN

LYVL

F--

CIYM

INLT

IGDL

LFVT

FLPF

W---

--SGS

LFFI

NTY

SSVL

F-LC

AISV

--G

IILSV

FIW

LFTL

ATAI

LFLI

----H

LTIV

IGFF

LVFF

VVVV

CNFL

----

QM

LFSV

VGVF

VVCF

LPHH

ACQ

--Q

ITW

LLM

SLN

CILD

PVVY

FFA-

--Zebrafi

sh-IY

MTN

LTVA

DLLF

VSAL

PFW

I----P

IFYG

VVFV

LGLI

ANCY

ALYV

----

GTFF

FIN

TYGS

ILF-

LTVI

SIN

RGAI

ISVL

IWAF

ILSA

SIPQ

L---

--LRM

LCAV

IGVF

VICF

LPHH

VV---

ITHF

SIIG

LFFL

VFLM

VIIC

N---

QIT

LMLM

GIN

CLLD

PLVY

CFA-

--

Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5087

23.2

4GF

YFGS

TVVA

FLAL

LL--F

FIFL

-AV

PLFV

LVIV

ALLI

TAFA

AIV-

----V

GNFI

AVVC

YTIF

CFLV

VSAV

--A

AYFS

LPIIA

DVV-

---YY

LSSL

VW---

--LCY

GFFF

LGLI

YPCI

SIAQ

WL-

-----T

FDTL

GAFF

FNGM

LLVS

LYFV

---LI

PVYA

ALLY

LLCA

M---

--FFT

VVY

Hum

an-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L---

-IHIF

IVFS

FF---

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

II---C

FVPH

H----

----

VTLC

LLST

NCV

LDPV

IYCF

LT--

Chic

ken

--TVF

YSIIF

ILGF

VAN

CYVL

WI-

--FM

LNLT

VADL

LFLV

TMPL

WIV

-SV

AGSL

FFVN

TYAS

VAFL

MVI

----G

IFLS

AAIW

IITVG

SALY

YIF-

---AI

HIIIC

TLFY

---VI

FLFI

LTW

N---

RALW

MVC

TVLV

VFII-

--SFV

PHH-

----

---VT

LCLL

SMN

CVLD

PVIY

CFLT

--Ch

impa

nzee

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

---IH

IFIV

FSFF

---LV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFII-

--CFV

PHH-

----

---VT

LCLL

STN

CVLD

PVIY

CFLT

--Co

w-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

FFFI

NTY

CSVA

FLAV

I---

--ILL

SLIIW

VSIV

GAAS

YFFV

L---

-IHIF

LVFS

FF---

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

II---C

FVPH

H----

----

VTLC

LLST

NCV

LDPI

IYCF

LT--

Elep

hant

-FPI

FYSI

IFVL

GVIA

NGY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

TLSL

VIW

VTIV

AAAS

YFLF

M---

--HIF

IVCS

FF---

LVFF

IILVC

NLV

-RA

LWM

VCTV

LAVF

II---C

FVPH

H----

---Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Frog

---VT

YSIIF

VFGF

IAN

CYVL

WIF

-IFM

INLT

IADL

LFLV

TLPL

WI--

NVA

GCFF

FLN

TYCS

VAFL

GVI--

----

CISV

AVW

ILLF

ASSL

YFLI

FP--

--HFI

LVGA

FF---

VVFL

IILTC

NLV

-RA

LWM

VVTV

LAVF

VL---

CFVP

HH---

-----

--LCL

MST

NCM

LDPI

IYCF

LTKK

Goat

-FPI

FYSI

VFVL

GVIA

NSY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCFF

FIN

TYCS

VAFL

AVI--

--IH

IFLV

FSFF

---LV

FLIIL

FCN

L--

--FLL

SLIIW

VSIV

GAAS

YFFV

L---

RALW

MVC

TVLA

VFVI

---CF

VPHH

-----

---VT

LCLL

STN

CVLD

PIIY

CFLT

--Gu

inea

pig

-FPI

VYSI

IFVL

GIIA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

ALSL

VIW

VAIV

AAAS

YFLV

M---

-IHIC

IVLG

FF---

IVFL

LILF

CNL-

-RA

LWM

VCTV

LAVF

VI---

CFVP

HH---

----Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Hedg

ehog

-FPI

FYSI

IFAL

GVIA

NSY

VLW

----F

MVN

LTLA

DLLF

LVTL

PLW

II-N

LAGC

FFFI

NTY

CSVA

FLGV

I---

-GIIL

SLVI

WVA

IVSA

ASYF

FF---

--IH

IFLV

VSFF

---VV

FLIIF

FCN

L--

RALW

MVC

TVLA

VFII-

--CFV

PHH-

----

--QIT

LCLL

STN

CVLD

PIIY

CFL-

--Ho

rse

--PIV

YSIIF

VLGV

IAN

SYVL

WV-

-IFM

VNLT

VADL

LFLL

TLPL

WV-

-N

LTGC

CFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WLA

IVAA

ASYF

LFM

----IH

IFLV

FSFF

---LV

FLIIL

VCN

L--

RALW

MVC

TVLA

VFII-

--CFV

PHH-

----

--QVT

LCLL

STN

CVLD

PIIY

CFL-

--M

edak

a-F

SVVY

SIFF

VVGL

LAN

VYVL

F--

--YM

TNLT

IADL

LFVC

ALPF

WIG

---T

GSFF

FIN

TYGT

ILFL

GAIS

L-RG

IIVSV

LIW

LCLV

SSAI

PSL-

----

--HFL

IIGLF

F---V

VFFL

VMVC

NLL

----

QM

LLAV

VGVF

LL---

CFLP

HHVV

Q--

--QIT

LVLM

GLN

CILD

PVIY

YFA-

--M

onke

y-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFFI

L---

-IHIF

IVFS

FF---

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

II---C

FVPH

H----

---Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Mou

se-F

PIVY

SVIF

ILGV

VAN

GYVL

W--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

-GIS

LSLI

IWVS

IVAT

ASYF

LA---

-VV

HVFI

AFCF

F---L

VFFL

IFYC

N---

RALW

MVC

TVLA

VFII-

--CFV

PHH-

----

---IT

LCLL

STN

CVLD

PVIY

CFLT

--O

poss

um-F

PIVY

SIIF

VLGF

VSN

AYVL

W--

---M

VNLT

VADL

LFLF

ALPL

WIV

YN

VAGC

LFFI

NTY

CSVC

FLGV

I---

-GIL

LSTI

IWVV

IVGC

ASYF

LI---

---H

VFIV

LCFF

---IV

FLVI

LVCN

VI-

--LW

MVC

TVM

AVFF

I---C

FVPH

HVV-

----Q

VTLC

LMSI

NCV

LDPI

IYCF

L---

Ora

ngut

an-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L---

-IHIF

IVFS

FF---

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

II---C

FVPH

H----

----

VTLC

LLST

NCV

LDPV

IYCF

LT--

Pig

-FPI

FYSI

IFVL

GVIA

NSY

VLW

-----

MLN

LTM

ADLL

FLVT

LPLW

IIYN

LAGC

FFFI

NTY

CSVS

FLAV

I---

--ISL

SLIIW

VAM

VAAA

SYFF

VL---

-IHIF

LVFS

FF---

LVFL

VILF

CNL-

-RA

LWM

VCTV

LAVF

II---C

FVPH

H----

----

ITLC

LLST

NCV

LDPI

IYCF

LT--

Pika

-FPI

VYSI

IFVL

GVVA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

II-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLIIW

VVIL

AIAS

YFLI

M---

-IHIF

IVFC

FF---

VVFL

IILVC

NL-

-RA

LWM

VCTV

LGVF

II---C

FVPH

H----

---Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Plat

ypus

---VA

YSIIF

VLGI

FAN

GYVL

WVF

--FM

VNLT

VADL

LFLV

TMPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

-GIV

LSLV

IWVL

IVSS

ALYF

LV---

--IH

IFIV

ASFF

---AV

FLLI

LVCN

L--

RALW

MVC

TVLA

VFII-

--CFV

PHH-

----

---VT

LCLL

STN

CVLD

PVIY

CFLT

--Pu

fferfish

--PSA

YGVI

FILG

LLAN

LYVL

FI-

---M

TNLS

IADL

LFVC

ALPF

WIG

Y---

GSLF

FIN

TYCS

VLFL

GAIS

VN-G

IIISV

IIWVL

TISM

AIPY

LV---

----

FAIIG

LFF-

--VVF

ALVL

VCN

LLI

-----L

LAVV

GVFV

V---C

FLPH

HVIQ

GA--Q

ITLL

LMGL

NCI

LDPV

VYCF

A---

Rabb

it--P

IVYS

IIFVL

GVVA

NGY

VLW

V---F

MVN

LTVA

DLLF

LITL

PLW

II-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--IAL

SLVI

WVS

ISAA

ATYF

LIL-

---IH

IFIV

ASFF

---IV

FLIIL

ICN

L--

RALW

MVC

TVLA

VFII-

--CFV

PHH-

----

--QVT

LCLL

STN

CVLD

PVIY

CFL-

--Ra

t--P

IVYS

VIFV

LGVV

ANGY

VLW

V---F

MVN

LTVA

DLLF

LMTL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

--G

ITLS

LVIW

ISIA

ATAS

YFLA

----

-VHI

FITS

CFF-

--LVF

FLIF

YCN

M--

RALW

MVC

TVLA

VFVI

---CF

VPHH

-----

---IT

LCLL

STN

CVLD

PVIY

CFLT

--Sh

rew

-FPI

FYSI

IFVL

GVIA

NSY

VLW

----F

MVN

LTM

ADLL

FLIT

LPLW

IV-

NLT

GCFF

FIN

TYCS

VAFL

GVI--

--G

IYLS

LVIW

MAI

VSAA

SYFF

F----

-IHIF

LVCS

FF---

LVFL

LIFF

CNL-

-RA

LWM

VCTV

LAVF

SI---

CFVP

HH---

-----

VTLC

LLST

NCV

LDPI

IYCF

LT--

Squi

rrel

LFPI

IYSI

IFVV

GVIA

NGY

VL---

--FM

LNLT

VADL

LFLI

TLPL

WII-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

----

YLSL

VIW

VFIV

AAAS

YFLI

LD--

VIHI

FIVF

SFF-

--LVF

LIIL

FCK-

--RA

LWM

VCAV

LAVF

VI---

CFVP

HH---

----Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Stickleba

ck-F

PVVY

SIIF

ILGI

TAN

LYVL

F--

CIYM

INLT

IGDL

LFVT

FLPF

W---

--SGS

LFFI

NTY

SSVL

FLCA

ISV-

-GIIL

SVFI

WLF

TLAT

AILF

LI---

---H

LTIV

IGFF

---LV

FFVV

VVCN

FL-

---Q

MLF

SVVG

VFVV

---CF

LPHH

ACQ

----Q

ITW

LLM

SLN

CILD

PVVY

FFA-

--Zebrafi

sh--P

IFYG

VVFV

LGLI

ANCY

ALYV

--IY

MTN

LTVA

DLLF

VSAL

PFW

I-----

GTFF

FIN

TYGS

ILFL

TVIS

INRG

AIIS

VLIW

AFIL

SASI

PQL-

----

--LRM

LCAV

IGVF

VI---

CFLP

HHVV

---IT

HFSI

IGLF

F---L

VFLM

VIIC

N---

--QIT

LMLM

GIN

CLLD

PLVY

CFA-

--


Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5097

91.6

0GF

YFGS

TVVA

FLAL

LL--F

FIFL

-AA

PLFV

LVIV

ALLI

TAFA

AIV-

----V

GNFI

AVVC

YTIF

CFLV

VSAV

--A

AYFS

LPIIA

DVV-

---YY

LSSL

VW---

--LCY

GFFF

LGLI

YPCI

SIAQ

WL-

-----T

FDTL

GAFF

FNGM

LLVS

LYFV

---LI

PVYA

ALLY

LLCA

M---

--FFT

VVY

Hum

an-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L---

-IHIF

IVFS

FF---

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

II---C

FVPH

H----

----

VTLC

LLST

NCV

LDPV

IYCF

LT--

Chic

ken

--TVF

YSIIF

ILGF

VAN

CYVL

WI-

--FM

LNLT

VADL

LFLV

TMPL

WIV

-SV

AGSL

FFVN

TYAS

VAFL

MVI

----G

IFLS

AAIW

IITVG

SALY

YIF-

---RA

LWM

VCTV

LVVF

II---S

FVPH

H----

-AI

HIIIC

TLFY

---VI

FLFI

LTW

N---

---VT

LCLL

SMN

CVLD

PVIY

CFLT

--Ch

impa

nzee

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

---IH

IFIV

FSFF

---LV

FLIIL

FCN

L--

---VT

LCLL

STN

CVLD

PVIY

CFLT

--RA

LWM

VCTV

LAVF

II---C

FVPH

H----

-Co

w-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

FFFI

NTY

CSVA

FLAV

I---

--ILL

SLIIW

VSIV

GAAS

YFFV

L---

-IHIF

LVFS

FF---

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

II---C

FVPH

H----

----

VTLC

LLST

NCV

LDPI

IYCF

LT--

Elep

hant

-FPI

FYSI

IFVL

GVIA

NGY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

TLSL

VIW

VTIV

AAAS

YFLF

M---

--HIF

IVCS

FF---

LVFF

IILVC

NLV

-RA

LWM

VCTV

LAVF

II---C

FVPH

H----

---Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Frog

---VT

YSIIF

VFGF

IAN

CYVL

WIF

-IFM

INLT

IADL

LFLV

TLPL

WI--

NVA

GCFF

FLN

TYCS

VAFL

GVI--

----

CISV

AVW

ILLF

ASSL

YFLI

FP--

--HFI

LVGA

FF---

VVFL

IILTC

NLV

-RA

LWM

VVTV

LAVF

VL---

CFVP

HH---

-----

--LCL

MST

NCM

LDPI

IYCF

LTKK

Goat

-FPI

FYSI

VFVL

GVIA

NSY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCFF

FIN

TYCS

VAFL

AVI--

---F

LLSL

IIWVS

IVGA

ASYF

FVL-

---IH

IFLV

FSFF

---LV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFVI

---CF

VPHH

-----

---VT

LCLL

STN

CVLD

PIIY

CFLT

--Gu

inea

pig

-FPI

VYSI

IFVL

GIIA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

ALSL

VIW

VAIV

AAAS

YFLV

M---

-IHIC

IVLG

FF---

IVFL

LILF

CNL-

-RA

LWM

VCTV

LAVF

VI---

CFVP

HH---

----Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Hedg

ehog

-FPI

FYSI

IFAL

GVIA

NSY

VLW

----F

MVN

LTLA

DLLF

LVTL

PLW

II-N

LAGC

FFFI

NTY

CSVA

FLGV

I---

-GIIL

SLVI

WVA

IVSA

ASYF

FF---

--IH

IFLV

VSFF

---VV

FLIIF

FCN

L--

RALW

MVC

TVLA

VFII-

--CFV

PHH-

----

--QIT

LCLL

STN

CVLD

PIIY

CFL-

--Ho

rse

--PIV

YSIIF

VLGV

IAN

SYVL

WV-

-IFM

VNLT

VADL

LFLL

TLPL

WV-

-N

LTGC

CFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WLA

IVAA

ASYF

LFM

----IH

IFLV

FSFF

---LV

FLIIL

VCN

L--

RALW

MVC

TVLA

VFII-

--CFV

PHH-

----

--QVT

LCLL

STN

CVLD

PIIY

CFL-

--M

edak

a-F

SVVY

SIFF

VVGL

LAN

VYVL

F--

--YM

TNLT

IADL

LFVC

ALPF

WIG

---T

GSFF

FIN

TYGT

ILFL

GAIS

L-RG

IIVSV

LIW

LCLV

SSAI

PSL-

----

--HFL

IIGLF

F---V

VFFL

VMVC

NLL

----

QM

LLAV

VGVF

LL---

CFLP

HHVV

Q--

--QIT

LVLM

GLN

CILD

PVIY

YFA-

--M

onke

y-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFFI

L---

-IHIF

IVFS

FF---

LVFL

IILFC

NL-

---Q

VTLC

LLST

NCV

LDPV

IYCF

L---

RALW

MVC

TVLA

VFII-

--CFV

PHH-

----

Mou

se-F

PIVY

SVIF

ILGV

VAN

GYVL

W--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

-GIS

LSLI

IWVS

IVAT

ASYF

LA---

-VV

HVFI

AFCF

F---L

VFFL

IFYC

N---

RALW

MVC

TVLA

VFII-

--CFV

PHH-

----

---IT

LCLL

STN

CVLD

PVIY

CFLT

--O

poss

um-F

PIVY

SIIF

VLGF

VSN

AYVL

W--

---M

VNLT

VADL

LFLF

ALPL

WIV

YN

VAGC

LFFI

NTY

CSVC

FLGV

I---

-GIL

LSTI

IWVV

IVGC

ASYF

LI---

---H

VFIV

LCFF

---IV

FLVI

LVCN

VI-

--LW

MVC

TVM

AVFF

I---C

FVPH

HVV-

----Q

VTLC

LMSI

NCV

LDPI

IYCF

L---

Ora

ngut

an-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L---

-IHIF

IVFS

FF---

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

II---C

FVPH

H----

----

VTLC

LLST

NCV

LDPV

IYCF

LT--

Pig

-FPI

FYSI

IFVL

GVIA

NSY

VLW

-----

MLN

LTM

ADLL

FLVT

LPLW

IIYN

LAGC

FFFI

NTY

CSVS

FLAV

I---

-IHIF

LVFS

FF---

LVFL

VILF

CNL-

---I

SLSL

IIWVA

MVA

AASY

FFVL

---RA

LWM

VCTV

LAVF

II---C

FVPH

H----

----

ITLC

LLST

NCV

LDPI

IYCF

LT--

Pika

-FPI

VYSI

IFVL

GVVA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

II-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLIIW

VVIL

AIAS

YFLI

M---

-IHIF

IVFC

FF---

VVFL

IILVC

NL-

-RA

LWM

VCTV

LGVF

II---C

FVPH

H----

---Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Plat

ypus

---VA

YSIIF

VLGI

FAN

GYVL

WVF

--FM

VNLT

VADL

LFLV

TMPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

-GIV

LSLV

IWVL

IVSS

ALYF

LV---

--IH

IFIV

ASFF

---AV

FLLI

LVCN

L--

RALW

MVC

TVLA

VFII-

--CFV

PHH-

----

---VT

LCLL

STN

CVLD

PVIY

CFLT

--Pu

fferfish

--PSA

YGVI

FILG

LLAN

LYVL

FI-

---M

TNLS

IADL

LFVC

ALPF

WIG

Y---

GSLF

FIN

TYCS

VLFL

GAIS

VN-G

IIISV

IIWVL

TISM

AIPY

LV---

----

FAIIG

LFF-

--VVF

ALVL

VCN

LLI

-----L

LAVV

GVFV

V---C

FLPH

HVIQ

GA--Q

ITLL

LMGL

NCI

LDPV

VYCF

A---

Rabb

it--P

IVYS

IIFVL

GVVA

NGY

VLW

V---F

MVN

LTVA

DLLF

LITL

PLW

II-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--IAL

SLVI

WVS

ISAA

ATYF

LIL-

---IH

IFIV

ASFF

---IV

FLIIL

ICN

L--

RALW

MVC

TVLA

VFII-

--CFV

PHH-

----

--QVT

LCLL

STN

CVLD

PVIY

CFL-

--Ra

t--P

IVYS

VIFV

LGVV

ANGY

VLW

V---F

MVN

LTVA

DLLF

LMTL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

--G

ITLS

LVIW

ISIA

ATAS

YFLA

----

-VHI

FITS

CFF-

--LVF

FLIF

YCN

M--

RALW

MVC

TVLA

VFVI

---CF

VPHH

-----

---IT

LCLL

STN

CVLD

PVIY

CFLT

--Sh

rew

-FPI

FYSI

IFVL

GVIA

NSY

VLW

----F

MVN

LTM

ADLL

FLIT

LPLW

IV-

NLT

GCFF

FIN

TYCS

VAFL

GVI--

--G

IYLS

LVIW

MAI

VSAA

SYFF

F----

-IHIF

LVCS

FF---

LVFL

LIFF

CNL-

-RA

LWM

VCTV

LAVF

SI---

CFVP

HH---

-----

VTLC

LLST

NCV

LDPI

IYCF

LT--

Squi

rrel

LFPI

IYSI

IFVV

GVIA

NGY

VL---

--FM

LNLT

VADL

LFLI

TLPL

WII-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

----

YLSL

VIW

VFIV

AAAS

YFLI

LD--

VIHI

FIVF

SFF-

--LVF

LIIL

FCK-

--RA

LWM

VCAV

LAVF

VI---

CFVP

HH---

----Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Stickleba

ck-F

PVVY

SIIF

ILGI

TAN

LYVL

F--

CIYM

INLT

IGDL

LFVT

FLPF

W---

--SGS

LFFI

NTY

SSVL

FLCA

ISV-

-GIIL

SVFI

WLF

TLAT

AILF

LI---

---H

LTIV

IGFF

---LV

FFVV

VVCN

FL-

---Q

MLF

SVVG

VFVV

---CF

LPHH

ACQ

----Q

ITW

LLM

SLN

CILD

PVVY

FFA-

--Zebrafi

sh--P

IFYG

VVFV

LGLI

ANCY

ALYV

--IY

MTN

LTVA

DLLF

VSAL

PFW

I-----

GTFF

FIN

TYGS

ILFL

TVIS

INRG

AIIS

VLIW

AFIL

SASI

PQL-

----

ITHF

SIIG

LFF-

--LVF

LMVI

ICN

-----L

RMLC

AVIG

VFVI

---CF

LPHH

VV---

--QIT

LMLM

GIN

CLLD

PLVY

CFA-

--

Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5067

53.3

0-L

EMVF

FIN

IVGC

LVPG

GACL

L--

RLFF

VVN

FVN

VLAT

YLM

FSSF

----

------

VSSS

TWVS

VFFV

CIGA

SLTS

G-S

LGAA

VIM

FTYI

LVFA

SNAA

L--

---SC

LVHF

FVLL

LSSI

TLLG

PVL

-----Q

IVVV

GISY

GVYQ

VFN

GVVL

FDS

VLQ

LLM

GLIL

LFAG

SYCY

V---

Hum

an-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

---FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GV---

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

--Ch

icke

n--T

VFYS

IIFIL

GFVA

NCY

VLW

I----

FMLN

LTVA

DLLF

LVTM

PLW

IV-

SVAG

SLFF

VNTY

ASVA

FLM

V---I

----G

IFLS

AAIW

IITVG

SALY

YIF-

-AI

HIIIC

TLFY

VIFL

FILT

WN

---RA

LWM

VCTV

LVVF

IISFV

PHH-

----

-VTL

CLLS

MN

CVLD

PVIY

CFLT

--Ch

impa

nzee

-FPI

VYSI

IFVL

GVIA

NGY

VLW

-----

FMVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

---I--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Cow

-FPI

FYSI

VFVL

GVIA

NSY

VLW

-----

-MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCFF

FIN

TYCS

VAFL

AV---

I---

--ILL

SLIIW

VSIV

GAAS

YFFV

L--IH

IFLV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPI

IYCF

LT--

Elep

hant

-FPI

FYSI

IFVL

GVIA

NGY

VLW

-----

-MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCLF

FIN

TYCS

VAFL

GV---

I---

--ITL

SLVI

WVT

IVAA

ASYF

LFM

---H

IFIV

CSFF

LVFF

IILVC

NLV

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--Fr

og---

VTYS

IIFVF

GFIA

NCY

VLW

IF--I

FMIN

LTIA

DLLF

LVTL

PLW

I--N

VAGC

FFFL

NTY

CSVA

FLGV

---I--

----

CISV

AVW

ILLF

ASSL

YFLI

FP--H

FILV

GAFF

VVFL

IILTC

NLV

-RA

LWM

VVTV

LAVF

VLCF

VPHH

-----

---LC

LMST

NCM

LDPI

IYCF

LTKK

Goat

-FPI

FYSI

VFVL

GVIA

NSY

VLW

-----

-MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCFF

FIN

TYCS

VAFL

AV---

I---

--FLL

SLIIW

VSIV

GAAS

YFFV

L--IH

IFLV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

VICF

VPHH

-----

-VTL

CLLS

TNCV

LDPI

IYCF

LT--

Guin

ea p

ig-F

PIVY

SIIF

VLGI

IAN

GYVL

W--

---FM

VNLT

VADL

LFLI

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

---I--

---I

ALSL

VIW

VAIV

AAAS

YFLV

M-

-IHIC

IVLG

FFIV

FLLI

LFCN

L--

RALW

MVC

TVLA

VFVI

CFVP

HH---

--Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Hedg

ehog

-FPI

FYSI

IFAL

GVIA

NSY

VLW

-----

FMVN

LTLA

DLLF

LVTL

PLW

II-N

LAGC

FFFI

NTY

CSVA

FLGV

---I--

--G

IILSL

VIW

VAIV

SAAS

YFFF

---IH

IFLV

VSFF

VVFL

IIFFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

QIT

LCLL

STN

CVLD

PIIY

CFL-

--Ho

rse

--PIV

YSIIF

VLGV

IAN

SYVL

WV-

--IFM

VNLT

VADL

LFLL

TLPL

WV-

-N

LTGC

CFFI

NTY

CSVA

FLGV

---I--

---I

SLSL

VIW

LAIV

AAAS

YFLF

M-

-IHIF

LVFS

FFLV

FLIIL

VCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPI

IYCF

L---

Med

aka

-FSV

VYSI

FFVV

GLLA

NVY

VLF-

----

YMTN

LTIA

DLLF

VCAL

PFW

IG-

--TGS

FFFI

NTY

GTIL

FLGA

---IS

L-RG

IIVSV

LIW

LCLV

SSAI

PSL-

----H

FLIIG

LFFV

VFFL

VMVC

NLL

----

QM

LLAV

VGVF

LLCF

LPHH

VVQ

--Q

ITLV

LMGL

NCI

LDPV

IYYF

A---

Mon

key

-FPI

VYSI

IFVL

GVIA

NGY

VLW

-----

FMVN

LTM

ADM

LFLI

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

---I--

---I

SLSL

VIW

VAIV

GAAS

YFFI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--M

ouse

-FPI

VYSV

IFIL

GVVA

NGY

VLW

-----

FMVN

LTM

ADLL

FLIT

LPLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GV---

I---

-GIS

LSLI

IWVS

IVAT

ASYF

LA--

VVHV

FIAF

CFFL

VFFL

IFYC

N---

RALW

MVC

TVLA

VFIIC

FVPH

H----

--IT

LCLL

STN

CVLD

PVIY

CFLT

--O

poss

um-F

PIVY

SIIF

VLGF

VSN

AYVL

W--

----M

VNLT

VADL

LFLF

ALPL

WIV

YN

VAGC

LFFI

NTY

CSVC

FLGV

---I--

--G

ILLS

TIIW

VVIV

GCAS

YFLI

----H

VFIV

LCFF

IVFL

VILV

CNVI

---L

WM

VCTV

MAV

FFIC

FVPH

HVV-

--Q

VTLC

LMSI

NCV

LDPI

IYCF

L---

Ora

ngut

an-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

---FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GV---

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

--Pi

g-F

PIFY

SIIF

VLGV

IAN

SYVL

W--

----M

LNLT

MAD

LLFL

VTLP

LWIIY

NLA

GCFF

FIN

TYCS

VSFL

AV---

I---

--ISL

SLIIW

VAM

VAAA

SYFF

VL-

-IHIF

LVFS

FFLV

FLVI

LFCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--IT

LCLL

STN

CVLD

PIIY

CFLT

--Pi

ka-F

PIVY

SIIF

VLGV

VAN

GYVL

W--

---FM

VNLT

VADL

LFLI

TLPL

WII-

NVA

GCLF

FIN

TYCS

VAFL

GV---

I---

--ISL

SLIIW

VVIL

AIAS

YFLI

M-

-IHIF

IVFC

FFVV

FLIIL

VCN

L--

RALW

MVC

TVLG

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Plat

ypus

---VA

YSIIF

VLGI

FAN

GYVL

WVF

---FM

VNLT

VADL

LFLV

TMPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

---I--

--G

IVLS

LVIW

VLIV

SSAL

YFLV

---IH

IFIV

ASFF

AVFL

LILV

CNL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Puffe

rfish

--PSA

YGVI

FILG

LLAN

LYVL

FI-

----M

TNLS

IADL

LFVC

ALPF

WIG

Y---

GSLF

FIN

TYCS

VLFL

GA---

ISVN

-GIII

SVIIW

VLTI

SMAI

PYLV

-----

FAIIG

LFFV

VFAL

VLVC

NLL

I---

--LLA

VVGV

FVVC

FLPH

HVIQ

GAQ

ITLL

LMGL

NCI

LDPV

VYCF

A---

Rabb

it--P

IVYS

IIFVL

GVVA

NGY

VLW

V----

FMVN

LTVA

DLLF

LITL

PLW

II-N

LAGC

LFFI

NTY

CSVA

FLGV

---I--

---I

ALSL

VIW

VSIS

AAAT

YFLI

L--IH

IFIV

ASFF

IVFL

IILIC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--Ra

t--P

IVYS

VIFV

LGVV

ANGY

VLW

V----

FMVN

LTVA

DLLF

LMTL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GV---

I---

-GIT

LSLV

IWIS

IAAT

ASYF

LA--

-VHI

FITS

CFFL

VFFL

IFYC

NM

--RA

LWM

VCTV

LAVF

VICF

VPHH

-----

-ITLC

LLST

NCV

LDPV

IYCF

LT--

Shre

w-F

PIFY

SIIF

VLGV

IAN

SYVL

W--

---FM

VNLT

MAD

LLFL

ITLP

LWIV

-N

LTGC

FFFI

NTY

CSVA

FLGV

---I--

--G

IYLS

LVIW

MAI

VSAA

SYFF

F--

-IHIF

LVCS

FFLV

FLLI

FFCN

L--

RALW

MVC

TVLA

VFSI

CFVP

HH---

---V

TLCL

LSTN

CVLD

PIIY

CFLT

--Sq

uirr

elLF

PIIY

SIIF

VVGV

IAN

GYVL

------

FMLN

LTVA

DLLF

LITL

PLW

II-N

VAGC

LFFI

NTY

CSVA

FLGV

---I--

----

YLSL

VIW

VFIV

AAAS

YFLI

LDVI

HIFI

VFSF

FLVF

LIIL

FCK-

--RA

LWM

VCAV

LAVF

VICF

VPHH

-----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--Sti

ckleba

ck-F

PVVY

SIIF

ILGI

TAN

LYVL

F--

-CIY

MIN

LTIG

DLLF

VTFL

PFW

-----S

GSLF

FIN

TYSS

VLFL

CA---

ISV-

-GIIL

SVFI

WLF

TLAT

AILF

LI--

--HLT

IVIG

FFLV

FFVV

VVCN

FL-

---Q

MLF

SVVG

VFVV

CFLP

HHAC

Q--

QIT

WLL

MSL

NCI

LDPV

VYFF

A---

Zebrafi

sh--P

IFYG

VVFV

LGLI

ANCY

ALYV

---I

YMTN

LTVA

DLLF

VSAL

PFW

I-----

GTFF

FIN

TYGS

ILFL

TV---

ISIN

RGAI

ISVL

IWAF

ILSA

SIPQ

L---

ITHF

SIIG

LFFL

VFLM

VIIC

N---

--LRM

LCAV

IGVF

VICF

LPHH

VV---

QIT

LMLM

GIN

CLLD

PLVY

CFA-

--


Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5051

93.7

4-K

DIM

FLL-

FSIG

MVF

GPHI

GYV-

---EG

YSPL

VSLI

LLAS

NGI

RILY

--ALA

LLFQ

AMF-

AILV

HTVL

LIM

------

-VLW

TLLT

TIVV

LLYY

ATIG

TVEV

VGYI

ALGI

EALL

VLPQ

ILR-

--TI

MLV

LTW

FLGD

IIKVI

YFLV

------

-PF

ILCG

I-FQ

LSLD

LVVI

GQLI

--Hu

man

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

----

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

--VFI

ICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Chic

ken

--TVF

YSIIF

ILGF

VAN

CYVL

WI-

--FM

LNLT

VADL

LFLV

TMPL

WIV

-SV

AGSL

FFVN

TYAS

VAFL

MVI

----G

IFLS

AAIW

IITVG

SALY

YIF-

-----

AIHI

IICTL

FYVI

FLFI

LTW

N---

RALW

MVC

TVLV

--VFI

ISFV

PHH-

----

-VTL

CLLS

MN

CVLD

PVIY

CFLT

--Ch

impa

nzee

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

----

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

--VFI

ICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Cow

-FPI

FYSI

VFVL

GVIA

NSY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCFF

FIN

TYCS

VAFL

AVI--

---I

LLSL

IIWVS

IVGA

ASYF

FVL-

----

-IHIF

LVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

--VFI

ICFV

PHH-

----

-VTL

CLLS

TNCV

LDPI

IYCF

LT--

Elep

hant

-FPI

FYSI

IFVL

GVIA

NGY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

TLSL

VIW

VTIV

AAAS

YFLF

M---

----H

IFIV

CSFF

LVFF

IILVC

NLV

-RA

LWM

VCTV

LA--V

FIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Frog

---VT

YSIIF

VFGF

IAN

CYVL

WIF

-IFM

INLT

IADL

LFLV

TLPL

WI--

NVA

GCFF

FLN

TYCS

VAFL

GVI--

----

CISV

AVW

ILLF

ASSL

YFLI

FP---

---H

FILV

GAFF

VVFL

IILTC

NLV

-RA

LWM

VVTV

LA--V

FVLC

FVPH

H----

----

LCLM

STN

CMLD

PIIY

CFLT

KKGo

at-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

FFFI

NTY

CSVA

FLAV

I---

--FLL

SLIIW

VSIV

GAAS

YFFV

L----

--IH

IFLV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LA--V

FVIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PIIY

CFLT

--Gu

inea

pig

-FPI

VYSI

IFVL

GIIA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

ALSL

VIW

VAIV

AAAS

YFLV

M---

---IH

ICIV

LGFF

IVFL

LILF

CNL-

-RA

LWM

VCTV

LA--V

FVIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Hedg

ehog

-FPI

FYSI

IFAL

GVIA

NSY

VLW

----F

MVN

LTLA

DLLF

LVTL

PLW

II-N

LAGC

FFFI

NTY

CSVA

FLGV

I---

-GIIL

SLVI

WVA

IVSA

ASYF

FF---

----IH

IFLV

VSFF

VVFL

IIFFC

NL-

-RA

LWM

VCTV

LA--V

FIIC

FVPH

H----

-Q

ITLC

LLST

NCV

LDPI

IYCF

L---

Hors

e--P

IVYS

IIFVL

GVIA

NSY

VLW

V--IF

MVN

LTVA

DLLF

LLTL

PLW

V--

NLT

GCCF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

LAIV

AAAS

YFLF

M---

---IH

IFLV

FSFF

LVFL

IILVC

NL-

-RA

LWM

VCTV

LA--V

FIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPI

IYCF

L---

Med

aka

-FSV

VYSI

FFVV

GLLA

NVY

VLF-

---Y

MTN

LTIA

DLLF

VCAL

PFW

IG-

--TGS

FFFI

NTY

GTIL

FLGA

ISL-

RGIIV

SVLI

WLC

LVSS

AIPS

L----

-----H

FLIIG

LFFV

VFFL

VMVC

NLL

----

QM

LLAV

VG--V

FLLC

FLPH

HVVQ

--Q

ITLV

LMGL

NCI

LDPV

IYYF

A---

Mon

key

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

FIL-

----

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

--VFI

ICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--M

ouse

-FPI

VYSV

IFIL

GVVA

NGY

VLW

--N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--FM

VNLT

MAD

LLFL

ITLP

LWIV

--G

ISLS

LIIW

VSIV

ATAS

YFLA

------

VVHV

FIAF

CFFL

VFFL

IFYC

N---

RALW

MVC

TVLA

--VFI

ICFV

PHH-

----

-ITLC

LLST

NCV

LDPV

IYCF

LT--

Opo

ssum

-FPI

VYSI

IFVL

GFVS

NAY

VLW

-----

MVN

LTVA

DLLF

LFAL

PLW

IVY

NVA

GCLF

FIN

TYCS

VCFL

GVI--

--G

ILLS

TIIW

VVIV

GCAS

YFLI

------

--HVF

IVLC

FFIV

FLVI

LVCN

VI-

--LW

MVC

TVM

A--V

FFIC

FVPH

HVV-

--Q

VTLC

LMSI

NCV

LDPI

IYCF

L---

Ora

ngut

an-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L----

--IH

IFIV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LA--V

FIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

--Pi

g-F

PIFY

SIIF

VLGV

IAN

SYVL

W--

---M

LNLT

MAD

LLFL

VTLP

LWIIY

NLA

GCFF

FIN

TYCS

VSFL

AVI--

--IH

IFLV

FSFF

LVFL

VILF

CNL-

---I

SLSL

IIWVA

MVA

AASY

FFVL

-----

RALW

MVC

TVLA

--VFI

ICFV

PHH-

----

-ITLC

LLST

NCV

LDPI

IYCF

LT--

Pika

-FPI

VYSI

IFVL

GVVA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

II-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLIIW

VVIL

AIAS

YFLI

M---

---IH

IFIV

FCFF

VVFL

IILVC

NL-

-RA

LWM

VCTV

LG--V

FIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Plat

ypus

---VA

YSIIF

VLGI

FAN

GYVL

WVF

--FM

VNLT

VADL

LFLV

TMPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

-GIV

LSLV

IWVL

IVSS

ALYF

LV---

----IH

IFIV

ASFF

AVFL

LILV

CNL-

-RA

LWM

VCTV

LA--V

FIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

--Pu

fferfish

--PSA

YGVI

FILG

LLAN

LYVL

FI-

---M

TNLS

IADL

LFVC

ALPF

WIG

Y---

GSLF

FIN

TYCS

VLFL

GAIS

VN-G

IIISV

IIWVL

TISM

AIPY

LV---

------

FAIIG

LFFV

VFAL

VLVC

NLL

I---

--LLA

VVG-

-VFV

VCFL

PHHV

IQGA

QIT

LLLM

GLN

CILD

PVVY

CFA-

--Ra

bbit

--PIV

YSIIF

VLGV

VAN

GYVL

WV-

--FM

VNLT

VADL

LFLI

TLPL

WII-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

ALSL

VIW

VSIS

AAAT

YFLI

L----

--IH

IFIV

ASFF

IVFL

IILIC

NL-

-RA

LWM

VCTV

LA--V

FIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Rat

--PIV

YSVI

FVLG

VVAN

GYVL

WV-

--FM

VNLT

VADL

LFLM

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

-GIT

LSLV

IWIS

IAAT

ASYF

LA---

---RA

LWM

VCTV

LA--V

FVIC

FVPH

H----

--V

HIFI

TSCF

FLVF

FLIF

YCN

M--

-ITLC

LLST

NCV

LDPV

IYCF

LT--

Shre

w-F

PIFY

SIIF

VLGV

IAN

SYVL

W--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

--G

IYLS

LVIW

MAI

VSAA

SYFF

F----

--N

LTGC

FFFI

NTY

CSVA

FLGV

I---

-IHIF

LVCS

FFLV

FLLI

FFCN

L--

RALW

MVC

TVLA

--VFS

ICFV

PHH-

----

-VTL

CLLS

TNCV

LDPI

IYCF

LT--

Squi

rrel

LFPI

IYSI

IFVV

GVIA

NGY

VL---

--FM

LNLT

VADL

LFLI

TLPL

WII-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

----

YLSL

VIW

VFIV

AAAS

YFLI

LD---

-VI

HIFI

VFSF

FLVF

LIIL

FCK-

--RA

LWM

VCAV

LA--V

FVIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Stickleba

ck-F

PVVY

SIIF

ILGI

TAN

LYVL

F--

CIYM

INLT

IGDL

LFVT

FLPF

W---

--SGS

LFFI

NTY

SSVL

FLCA

ISV-

-GIIL

SVFI

WLF

TLAT

AILF

LI---

-----H

LTIV

IGFF

LVFF

VVVV

CNFL

----

QM

LFSV

VG--V

FVVC

FLPH

HACQ

--Q

ITW

LLM

SLN

CILD

PVVY

FFA-

--Zebrafi

sh--P

IFYG

VVFV

LGLI

ANCY

ALYV

--IY

MTN

LTVA

DLLF

VSAL

PFW

I-----

GTFF

FIN

TYGS

ILFL

TVIS

INRG

AIIS

VLIW

AFIL

SASI

PQL-

------

ITHF

SIIG

LFFL

VFLM

VIIC

N---

--LRM

LCAV

IG--V

FVIC

FLPH

HVV-

--Q

ITLM

LMGI

NCL

LDPL

VYCF

A---

Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5034

63.2

0-L

STFK

GIIG

AYYG

LPFS

GTGS

-----

LPIM

ISCT

LLPL

VFLG

FLLR

I---

----C

LGFL

SDAF

VLM

FGIT

ALAS

Q---

FLGF

SILI

SCTR

LVSI

LILF

PSV

AVFP

IMFG

YAFC

GHVV

FGA-

------

--SRS

IVTL

FCTT

FGDN

IIDTF

L-F

GRLF

IGTY

LLL-

-FIC

NIL

NVA

Hum

an-F

PIVY

SII--

-FVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

F-LG

VI---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

-IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

--Ch

icke

n--T

VFYS

II---F

ILGF

VAN

CYVL

WI-

--FM

LNLT

VADL

LFLV

TMPL

WIV

-SV

AGSL

FFVN

TYAS

VAF-

LMVI

----G

IFLS

AAIW

IITVG

SALY

YIF-

-AI

HII-I

CTLF

YVIF

LFILT

WN

---RA

LWM

VCTV

LVVF

IISFV

PHH-

----

-VTL

CLLS

MN

CVLD

PVIY

CFLT

--Ch

impa

nzee

-FPI

VYSI

I---F

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAF-

LGVI

-----I

SLSL

VIW

VAIV

GAAS

YFLI

L--IH

IF-IV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Cow

-FPI

FYSI

V---F

VLGV

IAN

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

FFFI

NTY

CSVA

F-LA

VI---

--ILL

SLIIW

VSIV

GAAS

YFFV

L--IH

IF-L

VFSF

FLVF

LIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PIIY

CFLT

--El

epha

nt-F

PIFY

SII--

-FVL

GVIA

NGY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCLF

FIN

TYCS

VAF-

LGVI

-----I

TLSL

VIW

VTIV

AAAS

YFLF

M-

--HIF

-IVCS

FFLV

FFIIL

VCN

LV-

RALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Frog

---VT

YSII-

--FVF

GFIA

NCY

VLW

IF-IF

MIN

LTIA

DLLF

LVTL

PLW

I--N

VAGC

FFFL

NTY

CSVA

F-LG

VI---

--HFI

-LVG

AFFV

VFLI

ILTCN

LV-

---CI

SVAV

WIL

LFAS

SLYF

LIFP

RALW

MVV

TVLA

VFVL

CFVP

HH---

-----

LCLM

STN

CMLD

PIIY

CFLT

KKGo

at-F

PIFY

SIV-

--FVL

GVIA

NSY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCFF

FIN

TYCS

VAF-

LAVI

-----F

LLSL

IIWVS

IVGA

ASYF

FVL-

-IHIF

-LVF

SFFL

VFLI

ILFC

NL-

-RA

LWM

VCTV

LAVF

VICF

VPHH

-----

-VTL

CLLS

TNCV

LDPI

IYCF

LT--

Guin

ea p

ig-F

PIVY

SII--

-FVL

GIIA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAF-

LGVI

-----I

ALSL

VIW

VAIV

AAAS

YFLV

M-

-IHIC

-IVLG

FFIV

FLLI

LFCN

L--

RALW

MVC

TVLA

VFVI

CFVP

HH---

--Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Hedg

ehog

-FPI

FYSI

I---F

ALGV

IAN

SYVL

W--

--FM

VNLT

LADL

LFLV

TLPL

WII-

NLA

GCFF

FIN

TYCS

VAF-

LGVI

----G

IILSL

VIW

VAIV

SAAS

YFFF

---IH

IF-L

VVSF

FVVF

LIIF

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

ITLC

LLST

NCV

LDPI

IYCF

L---

Hors

e--P

IVYS

II---F

VLGV

IAN

SYVL

WV-

-IFM

VNLT

VADL

LFLL

TLPL

WV-

-N

LTGC

CFFI

NTY

CSVA

F-LG

VI---

--ISL

SLVI

WLA

IVAA

ASYF

LFM

--IH

IF-L

VFSF

FLVF

LIILV

CNL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PIIY

CFL-

--M

edak

a-F

SVVY

SIF-

--FVV

GLLA

NVY

VLF-

---Y

MTN

LTIA

DLLF

VCAL

PFW

IG-

RGIIV

SVLI

WLC

LVSS

AIPS

L---

--TGS

FFFI

NTY

GTIL

F-LG

AISL

----

HFLI

IGLF

FVVF

FLVM

VCN

LL-

---Q

MLL

AVVG

VFLL

CFLP

HHVV

Q--

QIT

LVLM

GLN

CILD

PVIY

YFA-

--M

onke

y-F

PIVY

SII--

-FVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

F-LG

VI---

--ISL

SLVI

WVA

IVGA

ASYF

FIL-

-IHIF

-IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Mou

se-F

PIVY

SVI--

-FIL

GVVA

NGY

VLW

----F

MVN

LTM

ADLL

FLIT

LPLW

IV-

NVA

GCLF

FIN

TYCS

VAF-

LGVI

----G

ISLS

LIIW

VSIV

ATAS

YFLA

--VV

HVF-

IAFC

FFLV

FFLI

FYCN

---RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-ITLC

LLST

NCV

LDPV

IYCF

LT--

Opo

ssum

-FPI

VYSI

I---F

VLGF

VSN

AYVL

W--

NVA

GCLF

FIN

TYCS

VCF-

LGVI

------

MVN

LTVA

DLLF

LFAL

PLW

IVY

-GIL

LSTI

IWVV

IVGC

ASYF

LI--

--HVF

-IVLC

FFIV

FLVI

LVCN

VI-

--LW

MVC

TVM

AVFF

ICFV

PHHV

V---

QVT

LCLM

SIN

CVLD

PIIY

CFL-

--O

rang

utan

-FPI

VYSI

I---F

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

NVA

GCLF

FIN

TYCS

VAF-

LGVI

----IH

IF-IV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Pig

-FPI

FYSI

I---F

VLGV

IAN

SYVL

W--

---M

LNLT

MAD

LLFL

VTLP

LWIIY

NLA

GCFF

FIN

TYCS

VSF-

LAVI

-----I

SLSL

IIWVA

MVA

AASY

FFVL

--IH

IF-L

VFSF

FLVF

LVIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--IT

LCLL

STN

CVLD

PIIY

CFLT

--Pi

ka-F

PIVY

SII--

-FVL

GVVA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

II-N

VAGC

LFFI

NTY

CSVA

F-LG

VI---

--ISL

SLIIW

VVIL

AIAS

YFLI

M-

-IHIF

-IVFC

FFVV

FLIIL

VCN

L--

RALW

MVC

TVLG

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Plat

ypus

---VA

YSII-

--FVL

GIFA

NGY

VLW

VF--F

MVN

LTVA

DLLF

LVTM

PLW

IV-

NVA

GCLF

FIN

TYCS

VAF-

LGVI

----G

IVLS

LVIW

VLIV

SSAL

YFLV

---IH

IF-IV

ASFF

AVFL

LILV

CNL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Puffe

rfish

--PSA

YGVI

---FI

LGLL

ANLY

VLFI

----

MTN

LSIA

DLLF

VCAL

PFW

IGY

---GS

LFFI

NTY

CSVL

F-LG

AISV

N-G

IIISV

IIWVL

TISM

AIPY

LV--

----F

AIIG

LFFV

VFAL

VLVC

NLL

I---

--LLA

VVGV

FVVC

FLPH

HVIQ

GAQ

ITLL

LMGL

NCI

LDPV

VYCF

A---

Rabb

it--P

IVYS

II---F

VLGV

VAN

GYVL

WV-

--FM

VNLT

VADL

LFLI

TLPL

WII-

NLA

GCLF

FIN

TYCS

VAF-

LGVI

-----I

ALSL

VIW

VSIS

AAAT

YFLI

L--IH

IF-IV

ASFF

IVFL

IILIC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--Ra

t--P

IVYS

VI---

FVLG

VVAN

GYVL

WV-

--FM

VNLT

VADL

LFLM

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

F-LG

VI---

-GIT

LSLV

IWIS

IAAT

ASYF

LA--

-VHI

F-IT

SCFF

LVFF

LIFY

CNM

--RA

LWM

VCTV

LAVF

VICF

VPHH

-----

-ITLC

LLST

NCV

LDPV

IYCF

LT--

Shre

w-F

PIFY

SII--

-FVL

GVIA

NSY

VLW

----F

MVN

LTM

ADLL

FLIT

LPLW

IV-

NLT

GCFF

FIN

TYCS

VAF-

LGVI

----G

IYLS

LVIW

MAI

VSAA

SYFF

F--

-IHIF

-LVC

SFFL

VFLL

IFFC

NL-

--V

TLCL

LSTN

CVLD

PIIY

CFLT

--RA

LWM

VCTV

LAVF

SICF

VPHH

-----

Squi

rrel

LFPI

IYSI

I---F

VVGV

IAN

GYVL

-----F

MLN

LTVA

DLLF

LITL

PLW

II-N

VAGC

LFFI

NTY

CSVA

F-LG

VI---

---YL

SLVI

WVF

IVAA

ASYF

LILD

VIHI

F-IV

FSFF

LVFL

IILFC

K---

RALW

MVC

AVLA

VFVI

CFVP

HH---

--Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Stickleba

ck-F

PVVY

SII--

-FIL

GITA

NLY

VLF-

-CI

YMIN

LTIG

DLLF

VTFL

PFW

-----S

GSLF

FIN

TYSS

VLF-

LCAI

SV-

-GIIL

SVFI

WLF

TLAT

AILF

LI--

--HLT

-IVIG

FFLV

FFVV

VVCN

FL-

---Q

MLF

SVVG

VFVV

CFLP

HHAC

Q--

QIT

WLL

MSL

NCI

LDPV

VYFF

A---

Zebrafi

sh--P

IFYG

VV---

FVLG

LIAN

CYAL

YV-

-IYM

TNLT

VADL

LFVS

ALPF

WI--

---GT

FFFI

NTY

GSIL

F-LT

VISI

NRG

AIIS

VLIW

AFIL

SASI

PQL-

---IT

HFSI

IGLF

FLVF

LMVI

ICN

-----L

RMLC

AVIG

VFVI

CFLP

HHVV

---Q

ITLM

LMGI

NCL

LDPL

VYCF

A---


Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5041

37.1

50--H

LVGF

LAFL

VVVV

CF-F

VIVL

IYF

LFAA

YSFG

CLM

CMLC

STVF

---YF

GITF

LVVG

SFLP

FCYF

SFA-

---W

TYLS

MIS

FFGV

FGVL

GPFF

K--

LFYV

CMVG

SGLF

PIVH

IYLL

L----

-VE

GLLL

MM

TLYG

VGVF

VYAF

Q---

-----

IWHV

FVLA

A----

-AFV

HFFN

TASI

YHu

man

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

--FFL

VFLI

ILFC

NL-

--R

ALW

MVC

TVLA

V--F

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT---

-Ch

icke

n--T

VFYS

IIFIL

GFVA

NCY

VLW

I---F

MLN

LTVA

DLLF

LVTM

PLW

IV-

SVAG

SLFF

VNTY

ASVA

FLM

VI---

-GIF

LSAA

IWIIT

VGSA

LYYI

F--

AIHI

IICTL

--FYV

IFLF

ILTW

N---

-RAL

WM

VCTV

LVV-

-FIIS

FVPH

H----

--V

TLCL

LSM

NCV

LDPV

IYCF

LT---

-Ch

impa

nzee

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

--FFL

VFLI

ILFC

NL-

--R

ALW

MVC

TVLA

V--F

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT---

-Co

w-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

NLA

GCFF

FIN

TYCS

VAFL

AVI--

----

MVN

LTM

ADLL

FLVT

LPLW

IVY

--ILL

SLIIW

VSIV

GAAS

YFFV

L--IH

IFLV

FS--F

FLVF

LIIL

FCN

L--

-RAL

WM

VCTV

LAV-

-FIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PIIY

CFLT

----

Elep

hant

-FPI

FYSI

IFVL

GVIA

NGY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

TLSL

VIW

VTIV

AAAS

YFLF

M-

--HIF

IVCS

--FFL

VFFI

ILVCN

LV-

-RAL

WM

VCTV

LAV-

-FIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L----

-Fr

og---

VTYS

IIFVF

GFIA

NCY

VLW

IF-IF

MIN

LTIA

DLLF

LVTL

PLW

I--N

VAGC

FFFL

NTY

CSVA

FLGV

I---

---CI

SVAV

WIL

LFAS

SLYF

LIFP

--HFI

LVGA

--FFV

VFLI

ILTCN

LV-

-RAL

WM

VVTV

LAV-

-FVL

CFVP

HH---

-----

LCLM

STN

CMLD

PIIY

CFLT

KK--

Goat

-FPI

FYSI

VFVL

GVIA

NSY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCFF

FIN

TYCS

VAFL

AVI--

---F

LLSL

IIWVS

IVGA

ASYF

FVL-

-IHIF

LVFS

--FFL

VFLI

ILFC

NL-

--R

ALW

MVC

TVLA

V--F

VICF

VPHH

-----

-VTL

CLLS

TNCV

LDPI

IYCF

LT---

-Gu

inea

pig

-FPI

VYSI

IFVL

GIIA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

ALSL

VIW

VAIV

AAAS

YFLV

M-

-IHIC

IVLG

--FFI

VFLL

ILFC

NL-

--R

ALW

MVC

TVLA

V--F

VICF

VPHH

-----

QVT

LCLL

STN

CVLD

PVIY

CFL-

----

Hedg

ehog

-FPI

FYSI

IFAL

GVIA

NSY

VLW

----F

MVN

LTLA

DLLF

LVTL

PLW

II-N

LAGC

FFFI

NTY

CSVA

FLGV

I---

-GIIL

SLVI

WVA

IVSA

ASYF

FF--

-IHIF

LVVS

--FFV

VFLI

IFFC

NL-

--R

ALW

MVC

TVLA

V--F

IICFV

PHH-

----

QIT

LCLL

STN

CVLD

PIIY

CFL-

----

Hors

e--P

IVYS

IIFVL

GVIA

NSY

VLW

V--IF

MVN

LTVA

DLLF

LLTL

PLW

V--

NLT

GCCF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

LAIV

AAAS

YFLF

M-

-IHIF

LVFS

--FFL

VFLI

ILVCN

L--

-RAL

WM

VCTV

LAV-

-FIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPI

IYCF

L----

-M

edak

a-F

SVVY

SIFF

VVGL

LAN

VYVL

F--

--YM

TNLT

IADL

LFVC

ALPF

WIG

-RG

IIVSV

LIW

LCLV

SSAI

PSL-

----T

GSFF

FIN

TYGT

ILFL

GAIS

L---H

FLIIG

L--F

FVVF

FLVM

VCN

LL-

----Q

MLL

AVVG

V--F

LLCF

LPHH

VVQ

--Q

ITLV

LMGL

NCI

LDPV

IYYF

A----

-M

onke

y-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFFI

L--IH

IFIV

FS--F

FLVF

LIIL

FCN

L--

-RAL

WM

VCTV

LAV-

-FIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L----

-M

ouse

-FPI

VYSV

IFIL

GVVA

NGY

VLW

----F

MVN

LTM

ADLL

FLIT

LPLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

--G

ISLS

LIIW

VSIV

ATAS

YFLA

--VV

HVFI

AFC-

-FFL

VFFL

IFYC

N---

-RAL

WM

VCTV

LAV-

-FIIC

FVPH

H----

--IT

LCLL

STN

CVLD

PVIY

CFLT

----

Opo

ssum

-FPI

VYSI

IFVL

GFVS

NAY

VLW

--Q

VTLC

LMSI

NCV

LDPI

IYCF

L----

----

MVN

LTVA

DLLF

LFAL

PLW

IVY

NVA

GCLF

FIN

TYCS

VCFL

GVI--

--G

ILLS

TIIW

VVIV

GCAS

YFLI

----H

VFIV

LC--F

FIVF

LVILV

CNVI

----

LWM

VCTV

MAV

--FFI

CFVP

HHVV

---O

rang

utan

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

--FFL

VFLI

ILFC

NL-

--R

ALW

MVC

TVLA

V--F

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT---

-Pi

g-F

PIFY

SIIF

VLGV

IAN

SYVL

W--

---M

LNLT

MAD

LLFL

VTLP

LWIIY

NLA

GCFF

FIN

TYCS

VSFL

AVI--

---I

SLSL

IIWVA

MVA

AASY

FFVL

--IH

IFLV

FS--F

FLVF

LVIL

FCN

L--

-RAL

WM

VCTV

LAV-

-FIIC

FVPH

H----

--IT

LCLL

STN

CVLD

PIIY

CFLT

----

Pika

-FPI

VYSI

IFVL

GVVA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

II-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLIIW

VVIL

AIAS

YFLI

M-

-IHIF

IVFC

--FFV

VFLI

ILVCN

L--

-RAL

WM

VCTV

LGV-

-FIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L----

-Pl

atyp

us---

VAYS

IIFVL

GIFA

NGY

VLW

VF--F

MVN

LTVA

DLLF

LVTM

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

--G

IVLS

LVIW

VLIV

SSAL

YFLV

---IH

IFIV

AS--F

FAVF

LLILV

CNL-

--R

ALW

MVC

TVLA

V--F

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT---

-Pu

fferfish

--PSA

YGVI

FILG

LLAN

LYVL

FI-

---M

TNLS

IADL

LFVC

ALPF

WIG

Y---

GSLF

FIN

TYCS

VLFL

GAIS

VN-G

IIISV

IIWVL

TISM

AIPY

LV--

---FA

IIGL-

-FFV

VFAL

VLVC

NLL

I---

---LL

AVVG

V--F

VVCF

LPHH

VIQ

GAQ

ITLL

LMGL

NCI

LDPV

VYCF

A----

-Ra

bbit

--PIV

YSIIF

VLGV

VAN

GYVL

WV-

--FM

VNLT

VADL

LFLI

TLPL

WII-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

ALSL

VIW

VSIS

AAAT

YFLI

L--IH

IFIV

AS--F

FIVF

LIIL

ICN

L--

-RAL

WM

VCTV

LAV-

-FIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L----

-Ra

t--P

IVYS

VIFV

LGVV

ANGY

VLW

V---F

MVN

LTVA

DLLF

LMTL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

--G

ITLS

LVIW

ISIA

ATAS

YFLA

---V

HIFI

TSC-

-FFL

VFFL

IFYC

NM

---R

ALW

MVC

TVLA

V--F

VICF

VPHH

-----

-ITLC

LLST

NCV

LDPV

IYCF

LT---

-Sh

rew

-FPI

FYSI

IFVL

GVIA

NSY

VLW

----F

MVN

LTM

ADLL

FLIT

LPLW

IV-

NLT

GCFF

FIN

TYCS

VAFL

GVI--

--G

IYLS

LVIW

MAI

VSAA

SYFF

F--

-IHIF

LVCS

--FFL

VFLL

IFFC

NL-

--R

ALW

MVC

TVLA

V--F

SICF

VPHH

-----

-VTL

CLLS

TNCV

LDPI

IYCF

LT---

-Sq

uirr

elLF

PIIY

SIIF

VVGV

IAN

GYVL

-----F

MLN

LTVA

DLLF

LITL

PLW

II-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

---YL

SLVI

WVF

IVAA

ASYF

LILD

VIHI

FIVF

S--F

FLVF

LIIL

FCK-

---R

ALW

MVC

AVLA

V--F

VICF

VPHH

-----

QVT

LCLL

STN

CVLD

PVIY

CFL-

----

Stickleba

ck-F

PVVY

SIIF

ILGI

TAN

LYVL

F--

CIYM

INLT

IGDL

LFVT

FLPF

W---

--SGS

LFFI

NTY

SSVL

FLCA

ISV-

-GIIL

SVFI

WLF

TLAT

AILF

LI--

--HLT

IVIG

--FFL

VFFV

VVVC

NFL

----

-QM

LFSV

VGV-

-FVV

CFLP

HHAC

Q--

QIT

WLL

MSL

NCI

LDPV

VYFF

A----

-Zebrafi

sh-IY

MTN

LTVA

DLLF

VSAL

PFW

I----P

IFYG

VVFV

LGLI

ANCY

ALYV

----

GTFF

FIN

TYGS

ILFL

TVIS

INRG

AIIS

VLIW

AFIL

SASI

PQL-

-----

LRM

LCAV

IGV-

-FVI

CFLP

HHVV

---IT

HFSI

IGL-

-FFL

VFLM

VIIC

N---

QIT

LMLM

GIN

CLLD

PLVY

CFA-

----

Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5064

67.4

-KDI

MFL

L-FS

IGM

VFGP

HIGY

V----

EGYS

PLVS

LILL

ASN

GIRI

LY--A

LALL

FQAM

F-AI

LVHT

VLLI

M---

----V

LWTL

LTTI

LVLL

YYAT

IGTV

EVVG

YIAL

GIEA

LLVL

PQIL

R---

TIM

LVLT

WFL

GDIIK

VIYF

LV---

----

PFIF

CGI-F

QLS

LDLV

VIGQ

LI--

Hum

an-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L----

--IH

IFIV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LA--V

FIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

--Ch

icke

n--T

VFYS

IIFIL

GFVA

NCY

VLW

I---F

MLN

LTVA

DLLF

LVTM

PLW

IV-

SVAG

SLFF

VNTY

ASVA

FLM

VI---

-GIF

LSAA

IWIIT

VGSA

LYYI

F----

--AI

HIIIC

TLFY

VIFL

FILT

WN

---RA

LWM

VCTV

LV--V

FIIS

FVPH

H----

--V

TLCL

LSM

NCV

LDPV

IYCF

LT--

Chim

panz

ee-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L----

--IH

IFIV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LA--V

FIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

--Co

w-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

FFFI

NTY

CSVA

FLAV

I---

--ILL

SLIIW

VSIV

GAAS

YFFV

L----

--IH

IFLV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LA--V

FIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PIIY

CFLT

--El

epha

nt-F

PIFY

SIIF

VLGV

IAN

GYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

LFFI

NTY

CSVA

FLGV

I---

--ITL

SLVI

WVT

IVAA

ASYF

LFM

-----

--HIF

IVCS

FFLV

FFIIL

VCN

LV-

RALW

MVC

TVLA

--VFI

ICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--Fr

og---

VTYS

IIFVF

GFIA

NCY

VLW

IF-IF

MIN

LTIA

DLLF

LVTL

PLW

I--N

VAGC

FFFL

NTY

CSVA

FLGV

I---

---CI

SVAV

WIL

LFAS

SLYF

LIFP

----

--HFI

LVGA

FFVV

FLIIL

TCN

LV-

RALW

MVV

TVLA

--VFV

LCFV

PHH-

----

---LC

LMST

NCM

LDPI

IYCF

LTKK

Goat

-FPI

FYSI

VFVL

GVIA

NSY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCFF

FIN

TYCS

VAFL

AVI--

--IH

IFLV

FSFF

LVFL

IILFC

NL-

---F

LLSL

IIWVS

IVGA

ASYF

FVL-

----

RALW

MVC

TVLA

--VFV

ICFV

PHH-

----

-VTL

CLLS

TNCV

LDPI

IYCF

LT--

Guin

ea p

ig-F

PIVY

SIIF

VLGI

IAN

GYVL

W--

--FM

VNLT

VADL

LFLI

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--IAL

SLVI

WVA

IVAA

ASYF

LVM

-----

-IHIC

IVLG

FFIV

FLLI

LFCN

L--

RALW

MVC

TVLA

--VFV

ICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--He

dgeh

og-F

PIFY

SIIF

ALGV

IAN

SYVL

W--

--FM

VNLT

LADL

LFLV

TLPL

WII-

NLA

GCFF

FIN

TYCS

VAFL

GVI--

--G

IILSL

VIW

VAIV

SAAS

YFFF

------

-IHIF

LVVS

FFVV

FLIIF

FCN

L--

RALW

MVC

TVLA

--VFI

ICFV

PHH-

----

QIT

LCLL

STN

CVLD

PIIY

CFL-

--Ho

rse

--PIV

YSIIF

VLGV

IAN

SYVL

WV-

-IFM

VNLT

VADL

LFLL

TLPL

WV-

-N

LTGC

CFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WLA

IVAA

ASYF

LFM

-----

-IHIF

LVFS

FFLV

FLIIL

VCN

L--

RALW

MVC

TVLA

--VFI

ICFV

PHH-

----

QVT

LCLL

STN

CVLD

PIIY

CFL-

--M

edak

a-F

SVVY

SIFF

VVGL

LAN

VYVL

F--

--YM

TNLT

IADL

LFVC

ALPF

WIG

---T

GSFF

FIN

TYGT

ILFL

GAIS

L-RG

IIVSV

LIW

LCLV

SSAI

PSL-

------

--HFL

IIGLF

FVVF

FLVM

VCN

LL-

---Q

MLL

AVVG

--VFL

LCFL

PHHV

VQ--

QIT

LVLM

GLN

CILD

PVIY

YFA-

--M

onke

y-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFFI

L----

--IH

IFIV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LA--V

FIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Mou

se-F

PIVY

SVIF

ILGV

VAN

GYVL

W--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

-GIS

LSLI

IWVS

IVAT

ASYF

LA---

---VV

HVFI

AFCF

FLVF

FLIF

YCN

---RA

LWM

VCTV

LA--V

FIIC

FVPH

H----

--IT

LCLL

STN

CVLD

PVIY

CFLT

--O

poss

um-F

PIVY

SIIF

VLGF

VSN

AYVL

W--

---M

VNLT

VADL

LFLF

ALPL

WIV

YN

VAGC

LFFI

NTY

CSVC

FLGV

I---

-GIL

LSTI

IWVV

IVGC

ASYF

LI---

-----H

VFIV

LCFF

IVFL

VILV

CNVI

---L

WM

VCTV

MA-

-VFF

ICFV

PHHV

V---

QVT

LCLM

SIN

CVLD

PIIY

CFL-

--O

rang

utan

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

----

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

--VFI

ICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Pig

-FPI

FYSI

IFVL

GVIA

NSY

VLW

-----

MLN

LTM

ADLL

FLVT

LPLW

IIYN

LAGC

FFFI

NTY

CSVS

FLAV

I---

--ISL

SLIIW

VAM

VAAA

SYFF

VL---

---IH

IFLV

FSFF

LVFL

VILF

CNL-

-RA

LWM

VCTV

LA--V

FIIC

FVPH

H----

--IT

LCLL

STN

CVLD

PIIY

CFLT

--Pi

ka-F

PIVY

SIIF

VLGV

VAN

GYVL

W--

--FM

VNLT

VADL

LFLI

TLPL

WII-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

IIWVV

ILAI

ASYF

LIM

-----

-IHIF

IVFC

FFVV

FLIIL

VCN

L--

RALW

MVC

TVLG

--VFI

ICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--Pl

atyp

us---

VAYS

IIFVL

GIFA

NGY

VLW

VF--F

MVN

LTVA

DLLF

LVTM

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

--G

IVLS

LVIW

VLIV

SSAL

YFLV

------

-IHIF

IVAS

FFAV

FLLI

LVCN

L--

RALW

MVC

TVLA

--VFI

ICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Puffe

rfish

--PSA

YGVI

FILG

LLAN

LYVL

FI-

---M

TNLS

IADL

LFVC

ALPF

WIG

Y---

GSLF

FIN

TYCS

VLFL

GAIS

VN-G

IIISV

IIWVL

TISM

AIPY

LV---

------

FAIIG

LFFV

VFAL

VLVC

NLL

I---

--LLA

VVG-

-VFV

VCFL

PHHV

IQGA

QIT

LLLM

GLN

CILD

PVVY

CFA-

--Ra

bbit

--PIV

YSIIF

VLGV

VAN

GYVL

WV-

--FM

VNLT

VADL

LFLI

TLPL

WII-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

ALSL

VIW

VSIS

AAAT

YFLI

L----

--IH

IFIV

ASFF

IVFL

IILIC

NL-

-RA

LWM

VCTV

LA--V

FIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Rat

--PIV

YSVI

FVLG

VVAN

GYVL

WV-

--FM

VNLT

VADL

LFLM

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

-GIT

LSLV

IWIS

IAAT

ASYF

LA---

----V

HIFI

TSCF

FLVF

FLIF

YCN

M--

RALW

MVC

TVLA

--VFV

ICFV

PHH-

----

-ITLC

LLST

NCV

LDPV

IYCF

LT--

Shre

w-F

PIFY

SIIF

VLGV

IAN

SYVL

W--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

-N

LTGC

FFFI

NTY

CSVA

FLGV

I---

-GIY

LSLV

IWM

AIVS

AASY

FFF-

-----

-IHIF

LVCS

FFLV

FLLI

FFCN

L--

RALW

MVC

TVLA

--VFS

ICFV

PHH-

----

-VTL

CLLS

TNCV

LDPI

IYCF

LT--

Squi

rrel

LFPI

IYSI

IFVV

GVIA

NGY

VL---

--FM

LNLT

VADL

LFLI

TLPL

WII-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

----

YLSL

VIW

VFIV

AAAS

YFLI

LD---

-VI

HIFI

VFSF

FLVF

LIIL

FCK-

--RA

LWM

VCAV

LA--V

FVIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Stickleba

ck-F

PVVY

SIIF

ILGI

TAN

LYVL

F--

CIYM

INLT

IGDL

LFVT

FLPF

W---

--SGS

LFFI

NTY

SSVL

FLCA

ISV-

-GIIL

SVFI

WLF

TLAT

AILF

LI---

------

QM

LFSV

VG--V

FVVC

FLPH

HACQ

----H

LTIV

IGFF

LVFF

VVVV

CNFL

-Q

ITW

LLM

SLN

CILD

PVVY

FFA-

--Zebrafi

sh--P

IFYG

VVFV

LGLI

ANCY

ALYV

--IY

MTN

LTVA

DLLF

VSAL

PFW

I-----

GTFF

FIN

TYGS

ILFL

TVIS

INRG

AIIS

VLIW

AFIL

SASI

PQL-

------

ITHF

SIIG

LFFL

VFLM

VIIC

N---

--LRM

LCAV

IG--V

FVIC

FLPH

HVV-

--Q

ITLM

LMGI

NCL

LDPL

VYCF

A---


Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5097

99.1

00--H

LVGF

LAFL

VVVV

CF-F

VIVL

IYF

LFAA

YSFG

CLM

CMLC

STVF

---YF

GITF

LVVG

SFLP

FCYF

SFA-

---W

TYLS

MIS

FFGV

FGVL

GPFF

K--

LFYV

CMVG

SGLF

PIVH

IYLL

L----

-VE

GLLL

MM

ALYG

VGVF

VYAF

Q---

-----

IWHV

FVLA

A----

-AFV

HFFN

TASI

YHu

man

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

--FFL

VFLI

ILFC

NL-

--V

TLCL

LSTN

CVLD

PVIY

CFLT

----

-RAL

WM

VCTV

LAV-

-FIIC

FVPH

H----

-Ch

icke

n--T

VFYS

IIFIL

GFVA

NCY

VLW

I---F

MLN

LTVA

DLLF

LVTM

PLW

IV-

SVAG

SLFF

VNTY

ASVA

FLM

VI---

-GIF

LSAA

IWIIT

VGSA

LYYI

F--

AIHI

IICTL

--FYV

IFLF

ILTW

N---

-RAL

WM

VCTV

LVV-

-FIIS

FVPH

H----

--V

TLCL

LSM

NCV

LDPV

IYCF

LT---

-Ch

impa

nzee

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

---I

SLSL

VIW

VAIV

GAAS

YFLI

L-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

-IHIF

IVFS

--FFL

VFLI

ILFC

NL-

--R

ALW

MVC

TVLA

V--F

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT---

-Co

w-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

FFFI

NTY

CSVA

FLAV

I---

--ILL

SLIIW

VSIV

GAAS

YFFV

L--IH

IFLV

FS--F

FLVF

LIIL

FCN

L--

-RAL

WM

VCTV

LAV-

-FIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PIIY

CFLT

----

Elep

hant

-FPI

FYSI

IFVL

GVIA

NGY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

TLSL

VIW

VTIV

AAAS

YFLF

M-

--HIF

IVCS

--FFL

VFFI

ILVCN

LV-

-RAL

WM

VCTV

LAV-

-FIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L----

-Fr

og---

VTYS

IIFVF

GFIA

NCY

VLW

IF-IF

MIN

LTIA

DLLF

LVTL

PLW

I--N

VAGC

FFFL

NTY

CSVA

FLGV

I---

--HFI

LVGA

--FFV

VFLI

ILTCN

LV-

---CI

SVAV

WIL

LFAS

SLYF

LIFP

-RAL

WM

VVTV

LAV-

-FVL

CFVP

HH---

-----

LCLM

STN

CMLD

PIIY

CFLT

KK--

Goat

-FPI

FYSI

VFVL

GVIA

NSY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCFF

FIN

TYCS

VAFL

AVI--

---F

LLSL

IIWVS

IVGA

ASYF

FVL-

-IHIF

LVFS

--FFL

VFLI

ILFC

NL-

--R

ALW

MVC

TVLA

V--F

VICF

VPHH

-----

-VTL

CLLS

TNCV

LDPI

IYCF

LT---

-Gu

inea

pig

-FPI

VYSI

IFVL

GIIA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

ALSL

VIW

VAIV

AAAS

YFLV

M-

-IHIC

IVLG

--FFI

VFLL

ILFC

NL-

--R

ALW

MVC

TVLA

V--F

VICF

VPHH

-----

QVT

LCLL

STN

CVLD

PVIY

CFL-

----

Hedg

ehog

-FPI

FYSI

IFAL

GVIA

NSY

VLW

----F

MVN

LTLA

DLLF

LVTL

PLW

II-N

LAGC

FFFI

NTY

CSVA

FLGV

I---

-GIIL

SLVI

WVA

IVSA

ASYF

FF--

-IHIF

LVVS

--FFV

VFLI

IFFC

NL-

--R

ALW

MVC

TVLA

V--F

IICFV

PHH-

----

QIT

LCLL

STN

CVLD

PIIY

CFL-

----

Hors

e--P

IVYS

IIFVL

GVIA

NSY

VLW

V--IF

MVN

LTVA

DLLF

LLTL

PLW

V--

NLT

GCCF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

LAIV

AAAS

YFLF

M-

-IHIF

LVFS

--FFL

VFLI

ILVCN

L--

-RAL

WM

VCTV

LAV-

-FIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPI

IYCF

L----

-M

edak

a-F

SVVY

SIFF

VVGL

LAN

VYVL

F--

--YM

TNLT

IADL

LFVC

ALPF

WIG

---T

GSFF

FIN

TYGT

ILFL

GAIS

L-RG

IIVSV

LIW

LCLV

SSAI

PSL-

----H

FLIIG

L--F

FVVF

FLVM

VCN

LL-

----Q

MLL

AVVG

V--F

LLCF

LPHH

VVQ

--Q

ITLV

LMGL

NCI

LDPV

IYYF

A----

-M

onke

y-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFFI

L--IH

IFIV

FS--F

FLVF

LIIL

FCN

L--

-RAL

WM

VCTV

LAV-

-FIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L----

-M

ouse

-FPI

VYSV

IFIL

GVVA

NGY

VLW

----F

MVN

LTM

ADLL

FLIT

LPLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

--G

ISLS

LIIW

VSIV

ATAS

YFLA

--VV

HVFI

AFC-

-FFL

VFFL

IFYC

N---

-RAL

WM

VCTV

LAV-

-FIIC

FVPH

H----

--IT

LCLL

STN

CVLD

PVIY

CFLT

----

Opo

ssum

-FPI

VYSI

IFVL

GFVS

NAY

VLW

-----

MVN

LTVA

DLLF

LFAL

PLW

IVY

NVA

GCLF

FIN

TYCS

VCFL

GVI--

--G

ILLS

TIIW

VVIV

GCAS

YFLI

----H

VFIV

LC--F

FIVF

LVILV

CNVI

----

LWM

VCTV

MAV

--FFI

CFVP

HHVV

---Q

VTLC

LMSI

NCV

LDPI

IYCF

L----

-O

rang

utan

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

--FFL

VFLI

ILFC

NL-

--R

ALW

MVC

TVLA

V--F

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT---

-Pi

g-F

PIFY

SIIF

VLGV

IAN

SYVL

W--

---M

LNLT

MAD

LLFL

VTLP

LWIIY

NLA

GCFF

FIN

TYCS

VSFL

AVI--

---I

SLSL

IIWVA

MVA

AASY

FFVL

--IH

IFLV

FS--F

FLVF

LVIL

FCN

L--

-RAL

WM

VCTV

LAV-

-FIIC

FVPH

H----

--IT

LCLL

STN

CVLD

PIIY

CFLT

----

Pika

-FPI

VYSI

IFVL

GVVA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

II-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLIIW

VVIL

AIAS

YFLI

M-

-IHIF

IVFC

--FFV

VFLI

ILVCN

L--

-RAL

WM

VCTV

LGV-

-FIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L----

-Pl

atyp

us---

VAYS

IIFVL

GIFA

NGY

VLW

VF--F

MVN

LTVA

DLLF

LVTM

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

--G

IVLS

LVIW

VLIV

SSAL

YFLV

---IH

IFIV

AS--F

FAVF

LLILV

CNL-

--R

ALW

MVC

TVLA

V--F

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT---

-Pu

fferfish

--PSA

YGVI

FILG

LLAN

LYVL

FI-

---M

TNLS

IADL

LFVC

ALPF

WIG

Y---

GSLF

FIN

TYCS

VLFL

GAIS

VN-G

IIISV

IIWVL

TISM

AIPY

LV--

---FA

IIGL-

-FFV

VFAL

VLVC

NLL

I---

---LL

AVVG

V--F

VVCF

LPHH

VIQ

GAQ

ITLL

LMGL

NCI

LDPV

VYCF

A----

-Ra

bbit

--PIV

YSIIF

VLGV

VAN

GYVL

WV-

--FM

VNLT

VADL

LFLI

TLPL

WII-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

ALSL

VIW

VSIS

AAAT

YFLI

L--IH

IFIV

AS--F

FIVF

LIIL

ICN

L--

-RAL

WM

VCTV

LAV-

-FIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L----

-Ra

t--P

IVYS

VIFV

LGVV

ANGY

VLW

V---F

MVN

LTVA

DLLF

LMTL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

--G

ITLS

LVIW

ISIA

ATAS

YFLA

---R

ALW

MVC

TVLA

V--F

VICF

VPHH

-----

-VHI

FITS

C--F

FLVF

FLIF

YCN

M--

-ITLC

LLST

NCV

LDPV

IYCF

LT---

-Sh

rew

-FPI

FYSI

IFVL

GVIA

NSY

VLW

----F

MVN

LTM

ADLL

FLIT

LPLW

IV-

NLT

GCFF

FIN

TYCS

VAFL

GVI--

--G

IYLS

LVIW

MAI

VSAA

SYFF

F--

-IHIF

LVCS

--FFL

VFLL

IFFC

NL-

--R

ALW

MVC

TVLA

V--F

SICF

VPHH

-----

-VTL

CLLS

TNCV

LDPI

IYCF

LT---

-Sq

uirr

elLF

PIIY

SIIF

VVGV

IAN

GYVL

-----F

MLN

LTVA

DLLF

LITL

PLW

II-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

---YL

SLVI

WVF

IVAA

ASYF

LILD

VIHI

FIVF

S--F

FLVF

LIIL

FCK-

---R

ALW

MVC

AVLA

V--F

VICF

VPHH

-----

QVT

LCLL

STN

CVLD

PVIY

CFL-

----

Stickleba

ck-F

PVVY

SIIF

ILGI

TAN

LYVL

F--

CIYM

INLT

IGDL

LFVT

FLPF

W---

--SGS

LFFI

NTY

SSVL

FLCA

ISV-

-GIIL

SVFI

WLF

TLAT

AILF

LI--

--HLT

IVIG

--FFL

VFFV

VVVC

NFL

----

-QM

LFSV

VGV-

-FVV

CFLP

HHAC

Q--

QIT

WLL

MSL

NCI

LDPV

VYFF

A----

-Zebrafi

sh-IY

MTN

LTVA

DLLF

VSAL

PFW

I----P

IFYG

VVFV

LGLI

ANCY

ALYV

----

GTFF

FIN

TYGS

ILFL

TVIS

INRG

AIIS

VLIW

AFIL

SASI

PQL-

-----

LRM

LCAV

IGV-

-FVI

CFLP

HHVV

---IT

HFSI

IGL-

-FFL

VFLM

VIIC

N---

QIT

LMLM

GIN

CLLD

PLVY

CFA-

----

Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5086

41.2

00---

-VYG

LLAA

GTAV

AGIG

SFLM

FS-

MPF

WLP

MVG

GFVP

LLW

LSFA

P----

---L

ALFF

SFTV

LEGM

ALAP

LVAI

-VL

GTAI

VLTG

AVFF

GFSA

AAL-

-----

-LQ

GPL-

FGM

LMGM

VAIS

ILN

LF-

FAHS

LILY

GGLA

LFSL

-FVS

VD---

--LQ

MFL

NVV

NIF

VRIA

QIL

GSM

----

Hum

an-F

PIVY

SIIF

VLGV

IA--N

GYVL

W--

--FM

VNLT

--MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

----

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

-RAL

WM

VCTV

LAVF

IICFV

PHH-

----

--VTL

CLLS

TNCV

LDPV

IYCF

LT--

Chic

ken

--FM

LNLT

--VAD

LLFL

VTM

PLW

IV-

--TVF

YSIIF

ILGF

VA--N

CYVL

WI-

SVAG

SLFF

VNTY

ASVA

FLM

VI---

----G

IFLS

AAIW

IITVG

SALY

YIF-

-AI

HIIIC

TLFY

VIFL

FILT

WN

-----V

TLCL

LSM

NCV

LDPV

IYCF

LT--

-RAL

WM

VCTV

LVVF

IISFV

PHH-

----

Chim

panz

ee-F

PIVY

SIIF

VLGV

IA--N

GYVL

W--

--FM

VNLT

--MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

----

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

-RAL

WM

VCTV

LAVF

IICFV

PHH-

----

--VTL

CLLS

TNCV

LDPV

IYCF

LT--

Cow

-FPI

FYSI

VFVL

GVIA

--NSY

VLW

--N

LAGC

FFFI

NTY

CSVA

FLAV

I---

---M

VNLT

--MAD

LLFL

VTLP

LWIV

Y---

--ILL

SLIIW

VSIV

GAAS

YFFV

L--IH

IFLV

FSFF

LVFL

IILFC

NL-

--R

ALW

MVC

TVLA

VFIIC

FVPH

H----

---V

TLCL

LSTN

CVLD

PIIY

CFLT

--El

epha

nt-F

PIFY

SIIF

VLGV

IA--N

GYVL

W--

---M

VNLT

--MAD

LLFL

VTLP

LWIV

YN

LAGC

LFFI

NTY

CSVA

FLGV

I---

-----I

TLSL

VIW

VTIV

AAAS

YFLF

M-

--HIF

IVCS

FFLV

FFIIL

VCN

LV-

-RAL

WM

VCTV

LAVF

IICFV

PHH-

----

-QVT

LCLL

STN

CVLD

PVIY

CFL-

--Fr

og---

VTYS

IIFVF

GFIA

--NCY

VLW

IF-IF

MIN

LT--I

ADLL

FLVT

LPLW

I--N

VAGC

FFFL

NTY

CSVA

FLGV

I---

------

CISV

AVW

ILLF

ASSL

YFLI

FP--H

FILV

GAFF

VVFL

IILTC

NLV

--R

ALW

MVV

TVLA

VFVL

CFVP

HH---

-----

-LCL

MST

NCM

LDPI

IYCF

LTKK

Goat

-FPI

FYSI

VFVL

GVIA

--NSY

VLW

-----

MVN

LT--M

ADLL

FLVT

LPLW

IVY

NLA

GCFF

FIN

TYCS

VAFL

AVI--

----

--FLL

SLIIW

VSIV

GAAS

YFFV

L--IH

IFLV

FSFF

LVFL

IILFC

NL-

--R

ALW

MVC

TVLA

VFVI

CFVP

HH---

----V

TLCL

LSTN

CVLD

PIIY

CFLT

--Gu

inea

pig

-FPI

VYSI

IFVL

GIIA

--NGY

VLW

----F

MVN

LT--V

ADLL

FLIT

LPLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

----

--IAL

SLVI

WVA

IVAA

ASYF

LVM

--IH

ICIV

LGFF

IVFL

LILF

CNL-

--R

ALW

MVC

TVLA

VFVI

CFVP

HH---

---Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Hedg

ehog

-FPI

FYSI

IFAL

GVIA

--NSY

VLW

----F

MVN

LT--L

ADLL

FLVT

LPLW

II-N

LAGC

FFFI

NTY

CSVA

FLGV

I---

----G

IILSL

VIW

VAIV

SAAS

YFFF

---IH

IFLV

VSFF

VVFL

IIFFC

NL-

--R

ALW

MVC

TVLA

VFIIC

FVPH

H----

--Q

ITLC

LLST

NCV

LDPI

IYCF

L---

Hors

e--P

IVYS

IIFVL

GVIA

--NSY

VLW

V--IF

MVN

LT--V

ADLL

FLLT

LPLW

V--

NLT

GCCF

FIN

TYCS

VAFL

GVI--

----

--ISL

SLVI

WLA

IVAA

ASYF

LFM

--IH

IFLV

FSFF

LVFL

IILVC

NL-

--R

ALW

MVC

TVLA

VFIIC

FVPH

H----

--Q

VTLC

LLST

NCV

LDPI

IYCF

L---

Med

aka

-FSV

VYSI

FFVV

GLLA

--NVY

VLF-

---Y

MTN

LT--I

ADLL

FVCA

LPFW

IG-

--TGS

FFFI

NTY

GTIL

FLGA

ISL-

---RG

IIVSV

LIW

LCLV

SSAI

PSL-

----H

FLIIG

LFFV

VFFL

VMVC

NLL

----

-QM

LLAV

VGVF

LLCF

LPHH

VVQ

---Q

ITLV

LMGL

NCI

LDPV

IYYF

A---

Mon

key

-FPI

VYSI

IFVL

GVIA

--NGY

VLW

----F

MVN

LT--M

ADM

LFLI

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

-----I

SLSL

VIW

VAIV

GAAS

YFFI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

--Q

VTLC

LLST

NCV

LDPV

IYCF

L---

-RAL

WM

VCTV

LAVF

IICFV

PHH-

----

Mou

se-F

PIVY

SVIF

ILGV

VA--N

GYVL

W--

--FM

VNLT

--MAD

LLFL

ITLP

LWIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

----G

ISLS

LIIW

VSIV

ATAS

YFLA

--VV

HVFI

AFCF

FLVF

FLIF

YCN

----R

ALW

MVC

TVLA

VFIIC

FVPH

H----

---I

TLCL

LSTN

CVLD

PVIY

CFLT

--O

poss

um-F

PIVY

SIIF

VLGF

VS--N

AYVL

W--

---M

VNLT

--VAD

LLFL

FALP

LWIV

YN

VAGC

LFFI

NTY

CSVC

FLGV

I---

----G

ILLS

TIIW

VVIV

GCAS

YFLI

----H

VFIV

LCFF

IVFL

VILV

CNVI

----

LWM

VCTV

MAV

FFIC

FVPH

HVV-

---Q

VTLC

LMSI

NCV

LDPI

IYCF

L---

Ora

ngut

an-F

PIVY

SIIF

VLGV

IA--N

GYVL

W--

--FM

VNLT

--MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

----

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

-RAL

WM

VCTV

LAVF

IICFV

PHH-

----

--VTL

CLLS

TNCV

LDPV

IYCF

LT--

Pig

-FPI

FYSI

IFVL

GVIA

--NSY

VLW

-----

MLN

LT--M

ADLL

FLVT

LPLW

IIYN

LAGC

FFFI

NTY

CSVS

FLAV

I---

-IHIF

LVFS

FFLV

FLVI

LFCN

L--

-----I

SLSL

IIWVA

MVA

AASY

FFVL

--R

ALW

MVC

TVLA

VFIIC

FVPH

H----

---I

TLCL

LSTN

CVLD

PIIY

CFLT

--Pi

ka-F

PIVY

SIIF

VLGV

VA--N

GYVL

W--

--FM

VNLT

--VAD

LLFL

ITLP

LWII-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

----

--ISL

SLIIW

VVIL

AIAS

YFLI

M-

-IHIF

IVFC

FFVV

FLIIL

VCN

L--

-RAL

WM

VCTV

LGVF

IICFV

PHH-

----

-QVT

LCLL

STN

CVLD

PVIY

CFL-

--Pl

atyp

us---

VAYS

IIFVL

GIFA

--NGY

VLW

VF--F

MVN

LT--V

ADLL

FLVT

MPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

----G

IVLS

LVIW

VLIV

SSAL

YFLV

---IH

IFIV

ASFF

AVFL

LILV

CNL-

--R

ALW

MVC

TVLA

VFIIC

FVPH

H----

---V

TLCL

LSTN

CVLD

PVIY

CFLT

--Pu

fferfish

--PSA

YGVI

FILG

LLA-

-NLY

VLFI

----

MTN

LS--I

ADLL

FVCA

LPFW

IGY

---GS

LFFI

NTY

CSVL

FLGA

ISVN

----G

IIISV

IIWVL

TISM

AIPY

LV--

---FA

IIGLF

FVVF

ALVL

VCN

LLI

------

LLAV

VGVF

VVCF

LPHH

VIQ

GA-Q

ITLL

LMGL

NCI

LDPV

VYCF

A---

Rabb

it--P

IVYS

IIFVL

GVVA

--NGY

VLW

V---F

MVN

LT--V

ADLL

FLIT

LPLW

II-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

-----I

ALSL

VIW

VSIS

AAAT

YFLI

L--IH

IFIV

ASFF

IVFL

IILIC

NL-

--R

ALW

MVC

TVLA

VFIIC

FVPH

H----

--Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Rat

--PIV

YSVI

FVLG

VVA-

-NGY

VLW

V---F

MVN

LT--V

ADLL

FLM

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

----G

ITLS

LVIW

ISIA

ATAS

YFLA

---V

HIFI

TSCF

FLVF

FLIF

YCN

M--

-RAL

WM

VCTV

LAVF

VICF

VPHH

-----

--ITL

CLLS

TNCV

LDPV

IYCF

LT--

Shre

w-F

PIFY

SIIF

VLGV

IA--N

SYVL

W--

--FM

VNLT

--MAD

LLFL

ITLP

LWIV

-N

LTGC

FFFI

NTY

CSVA

FLGV

I---

----G

IYLS

LVIW

MAI

VSAA

SYFF

F--

-IHIF

LVCS

FFLV

FLLI

FFCN

L--

-RAL

WM

VCTV

LAVF

SICF

VPHH

-----

--VTL

CLLS

TNCV

LDPI

IYCF

LT--

Squi

rrel

LFPI

IYSI

IFVV

GVIA

--NGY

VL---

--FM

LNLT

--VAD

LLFL

ITLP

LWII-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

----

---YL

SLVI

WVF

IVAA

ASYF

LILD

VIHI

FIVF

SFFL

VFLI

ILFC

K---

-RAL

WM

VCAV

LAVF

VICF

VPHH

-----

-QVT

LCLL

STN

CVLD

PVIY

CFL-

--Sti

ckleba

ck-F

PVVY

SIIF

ILGI

TA--N

LYVL

F--

CIYM

INLT

--IGD

LLFV

TFLP

FW---

--SGS

LFFI

NTY

SSVL

FLCA

ISV-

----G

IILSV

FIW

LFTL

ATAI

LFLI

----H

LTIV

IGFF

LVFF

VVVV

CNFL

----

-QM

LFSV

VGVF

VVCF

LPHH

ACQ

---Q

ITW

LLM

SLN

CILD

PVVY

FFA-

--Zebrafi

sh-IY

MTN

LT--V

ADLL

FVSA

LPFW

I----P

IFYG

VVFV

LGLI

A--N

CYAL

YV-

---GT

FFFI

NTY

GSIL

FLTV

ISIN

---RG

AIIS

VLIW

AFIL

SASI

PQL-

-----

LRM

LCAV

IGVF

VICF

LPHH

VV---

ITHF

SIIG

LFFL

VFLM

VIIC

N---

-QIT

LMLM

GIN

CLLD

PLVY

CFA-

--


Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5117

87.3

0GA

CEAH

LSM

AIAI

LFDL

LRSR

------

--DW

LMLG

LGGV

W---

-LPP

WSI

SFAL

FHCW

KALG

GKIL

FFFF

F---F

FME-

----

--STN

ALLQ

FLLA

VGGF

GVYS

NQ

--L

VIVA

SCM

TLTT

FLRI

SLCC

V--

MLL

IVVL

VFTA

VLDV

GNIL

LL---

--GV

SVTV

IFLG

ATAL

FGCV

IRL-

----

Hum

an---

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

----

-----N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

----

VTLC

LLST

NCV

LDPV

IYCF

LT--

Chic

ken

-----T

VFYS

IIFIL

GFVA

NCY

VLW

I---F

MLN

LTVA

DLLF

LVTM

PLW

IV---

----

--SVA

GSLF

FVN

TYAS

VAFL

MVI

----G

IFLS

AAIW

IITVG

SALY

YIF-

-AI

HIIIC

TLFY

VIFL

FILT

WN

---RA

LWM

VCTV

LVVF

IISFV

PHH-

----

---VT

LCLL

SMN

CVLD

PVIY

CFLT

--Ch

impa

nzee

----F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV---

----

--NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

---VT

LCLL

STN

CVLD

PVIY

CFLT

--Co

w---

-FPI

FYSI

VFVL

GVIA

NSY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY-

-----

--NLA

GCFF

FIN

TYCS

VAFL

AVI--

---I

LLSL

IIWVS

IVGA

ASYF

FVL-

-IHIF

LVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

----

VTLC

LLST

NCV

LDPI

IYCF

LT--

Elep

hant

----F

PIFY

SIIF

VLGV

IAN

GYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

Y---

-----N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--ITL

SLVI

WVT

IVAA

ASYF

LFM

---H

IFIV

CSFF

LVFF

IILVC

NLV

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

--QVT

LCLL

STN

CVLD

PVIY

CFL-

--Fr

og---

---VT

YSIIF

VFGF

IAN

CYVL

WIF

-IFM

INLT

IADL

LFLV

TLPL

WI--

------

--NVA

GCFF

FLN

TYCS

VAFL

GVI--

----

CISV

AVW

ILLF

ASSL

YFLI

FP--H

FILV

GAFF

VVFL

IILTC

NLV

-RA

LWM

VVTV

LAVF

VLCF

VPHH

-----

-----L

CLM

STN

CMLD

PIIY

CFLT

KKGo

at---

-FPI

FYSI

VFVL

GVIA

NSY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY-

-----

--NLA

GCFF

FIN

TYCS

VAFL

AVI--

---F

LLSL

IIWVS

IVGA

ASYF

FVL-

-IHIF

LVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFVI

CFVP

HH---

-----

VTLC

LLST

NCV

LDPI

IYCF

LT--

Guin

ea p

ig---

-FPI

VYSI

IFVL

GIIA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

IV---

----

--NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

ALSL

VIW

VAIV

AAAS

YFLV

M-

-IHIC

IVLG

FFIV

FLLI

LFCN

L--

RALW

MVC

TVLA

VFVI

CFVP

HH---

----Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Hedg

ehog

----F

PIFY

SIIF

ALGV

IAN

SYVL

W--

--FM

VNLT

LADL

LFLV

TLPL

WII-

------

--NLA

GCFF

FIN

TYCS

VAFL

GVI--

--G

IILSL

VIW

VAIV

SAAS

YFFF

---IH

IFLV

VSFF

VVFL

IIFFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

--QIT

LCLL

STN

CVLD

PIIY

CFL-

--Ho

rse

-----P

IVYS

IIFVL

GVIA

NSY

VLW

V--IF

MVN

LTVA

DLLF

LLTL

PLW

V----

----

--NLT

GCCF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

LAIV

AAAS

YFLF

M-

-IHIF

LVFS

FFLV

FLIIL

VCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

---Q

VTLC

LLST

NCV

LDPI

IYCF

L---

Med

aka

----F

SVVY

SIFF

VVGL

LAN

VYVL

F--

--YM

TNLT

IADL

LFVC

ALPF

WIG

----

------

-TGS

FFFI

NTY

GTIL

FLGA

ISL-

RGIIV

SVLI

WLC

LVSS

AIPS

L---

--HFL

IIGLF

FVVF

FLVM

VCN

LL-

---Q

MLL

AVVG

VFLL

CFLP

HHVV

Q--

--QIT

LVLM

GLN

CILD

PVIY

YFA-

--M

onke

y---

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

----

-----N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

FIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

---Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Mou

se---

-FPI

VYSV

IFIL

GVVA

NGY

VLW

----F

MVN

LTM

ADLL

FLIT

LPLW

IV---

----

--NVA

GCLF

FIN

TYCS

VAFL

GVI--

--G

ISLS

LIIW

VSIV

ATAS

YFLA

--VV

HVFI

AFCF

FLVF

FLIF

YCN

---RA

LWM

VCTV

LAVF

IICFV

PHH-

----

---IT

LCLL

STN

CVLD

PVIY

CFLT

--O

poss

um---

-FPI

VYSI

IFVL

GFVS

NAY

VLW

-----

MVN

LTVA

DLLF

LFAL

PLW

IVY-

-----

--NVA

GCLF

FIN

TYCS

VCFL

GVI--

--G

ILLS

TIIW

VVIV

GCAS

YFLI

----H

VFIV

LCFF

IVFL

VILV

CNVI

---L

WM

VCTV

MAV

FFIC

FVPH

HVV-

----Q

VTLC

LMSI

NCV

LDPI

IYCF

L---

Ora

ngut

an---

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

----

-----N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

---VT

LCLL

STN

CVLD

PVIY

CFLT

--RA

LWM

VCTV

LAVF

IICFV

PHH-

----

Pig

----F

PIFY

SIIF

VLGV

IAN

SYVL

W--

---M

LNLT

MAD

LLFL

VTLP

LWIIY

------

--NLA

GCFF

FIN

TYCS

VSFL

AVI--

---I

SLSL

IIWVA

MVA

AASY

FFVL

--IH

IFLV

FSFF

LVFL

VILF

CNL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

---IT

LCLL

STN

CVLD

PIIY

CFLT

--Pi

ka---

-FPI

VYSI

IFVL

GVVA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

II----

-----N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLIIW

VVIL

AIAS

YFLI

M-

-IHIF

IVFC

FFVV

FLIIL

VCN

L--

RALW

MVC

TVLG

VFIIC

FVPH

H----

---Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Plat

ypus

------

VAYS

IIFVL

GIFA

NGY

VLW

VF--F

MVN

LTVA

DLLF

LVTM

PLW

IV---

----

--NVA

GCLF

FIN

TYCS

VAFL

GVI--

--IH

IFIV

ASFF

AVFL

LILV

CNL-

--G

IVLS

LVIW

VLIV

SSAL

YFLV

--RA

LWM

VCTV

LAVF

IICFV

PHH-

----

---VT

LCLL

STN

CVLD

PVIY

CFLT

--Pu

fferfish

-----P

SAYG

VIFI

LGLL

ANLY

VLFI

----

MTN

LSIA

DLLF

VCAL

PFW

IGY-

-----

-----G

SLFF

INTY

CSVL

FLGA

ISVN

-GIII

SVIIW

VLTI

SMAI

PYLV

-----

FAIIG

LFFV

VFAL

VLVC

NLL

I---

--LLA

VVGV

FVVC

FLPH

HVIQ

GA--Q

ITLL

LMGL

NCI

LDPV

VYCF

A---

Rabb

it---

--PIV

YSIIF

VLGV

VAN

GYVL

WV-

--FM

VNLT

VADL

LFLI

TLPL

WII-

------

--NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

ALSL

VIW

VSIS

AAAT

YFLI

L--IH

IFIV

ASFF

IVFL

IILIC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

--QVT

LCLL

STN

CVLD

PVIY

CFL-

--Ra

t---

--PIV

YSVI

FVLG

VVAN

GYVL

WV-

--FM

VNLT

VADL

LFLM

TLPL

WIV

----

-----N

LAGC

LFFI

NTY

CSVA

FLGV

I---

-GIT

LSLV

IWIS

IAAT

ASYF

LA--

-VHI

FITS

CFFL

VFFL

IFYC

NM

--RA

LWM

VCTV

LAVF

VICF

VPHH

-----

---IT

LCLL

STN

CVLD

PVIY

CFLT

--Sh

rew

----F

PIFY

SIIF

VLGV

IAN

SYVL

W--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

----

-----N

LTGC

FFFI

NTY

CSVA

FLGV

I---

-GIY

LSLV

IWM

AIVS

AASY

FFF-

--IH

IFLV

CSFF

LVFL

LIFF

CNL-

-RA

LWM

VCTV

LAVF

SICF

VPHH

-----

---VT

LCLL

STN

CVLD

PIIY

CFLT

--Sq

uirr

el---

LFPI

IYSI

IFVV

GVIA

NGY

VL---

--FM

LNLT

VADL

LFLI

TLPL

WII-

------

--NVA

GCLF

FIN

TYCS

VAFL

GVI--

----

YLSL

VIW

VFIV

AAAS

YFLI

LDVI

HIFI

VFSF

FLVF

LIIL

FCK-

--RA

LWM

VCAV

LAVF

VICF

VPHH

-----

--QVT

LCLL

STN

CVLD

PVIY

CFL-

--Sti

ckleba

ck---

-FPV

VYSI

IFIL

GITA

NLY

VLF-

-CI

YMIN

LTIG

DLLF

VTFL

PFW

------

------

-SGS

LFFI

NTY

SSVL

FLCA

ISV-

-GIIL

SVFI

WLF

TLAT

AILF

LI--

--HLT

IVIG

FFLV

FFVV

VVCN

FL-

---Q

MLF

SVVG

VFVV

CFLP

HHAC

Q--

--QIT

WLL

MSL

NCI

LDPV

VYFF

A---

Zebrafi

sh---

--PIF

YGVV

FVLG

LIAN

CYAL

YV-

-IYM

TNLT

VADL

LFVS

ALPF

WI--

------

-----G

TFFF

INTY

GSIL

FLTV

ISIN

RGAI

ISVL

IWAF

ILSA

SIPQ

L---

ITHF

SIIG

LFFL

VFLM

VIIC

N---

--LRM

LCAV

IGVF

VICF

LPHH

VV---

--QIT

LMLM

GIN

CLLD

PLVY

CFA-

--

Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5103

05.7

9--T

TTLN

VVLP

AELL

YVVY

YLAY

----

-NAL

VASH

VALA

LFAS

LHVV

PA---

----Y

VPLV

MSL

VCIG

VLVL

CFVL

HSA

FLTY

LGFG

VLLY

GFAA

AAL-

-----

VFCL

RGLP

LVN

LLTG

VQLV

VSW

GAVQ

LLVI

VVLL

AIAV

LFYG

-----

ATFI

TIFF

IDVA

ALL-

-VFF

PVI--

-Hu

man

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

--N

VAGC

LFFI

NTY

CSVA

FLGV

I-----

----I

SLSL

VIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

---VT

LCLL

STN

CVLD

PVIY

CFLT

--Ch

icke

n--T

VFYS

IIFIL

GFVA

NCY

VLW

I---F

MLN

LTVA

DLLF

LVTM

PLW

IV--

SVAG

SLFF

VNTY

ASVA

FLM

VI---

----

-GIF

LSAA

IWIIT

VGSA

LYYI

F--

AIHI

IICTL

FYVI

FLFI

LTW

N---

RALW

MVC

TVLV

VFIIS

FVPH

H----

----

VTLC

LLSM

NCV

LDPV

IYCF

LT--

Chim

panz

ee-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV--

NVA

GCLF

FIN

TYCS

VAFL

GVI--

-----

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

----

VTLC

LLST

NCV

LDPV

IYCF

LT--

Cow

-FPI

FYSI

VFVL

GVIA

NSY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY-

NLA

GCFF

FIN

TYCS

VAFL

AVI--

-----

--ILL

SLIIW

VSIV

GAAS

YFFV

L--IH

IFLV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

---VT

LCLL

STN

CVLD

PIIY

CFLT

--El

epha

nt-F

PIFY

SIIF

VLGV

IAN

GYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

Y-N

LAGC

LFFI

NTY

CSVA

FLGV

I-----

----I

TLSL

VIW

VTIV

AAAS

YFLF

M-

--HIF

IVCS

FFLV

FFIIL

VCN

LV-

RALW

MVC

TVLA

VFIIC

FVPH

H----

---Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Frog

---VT

YSIIF

VFGF

IAN

CYVL

WIF

-IFM

INLT

IADL

LFLV

TLPL

WI--

-N

VAGC

FFFL

NTY

CSVA

FLGV

I-----

-----

CISV

AVW

ILLF

ASSL

YFLI

FP--H

FILV

GAFF

VVFL

IILTC

NLV

-RA

LWM

VVTV

LAVF

VLCF

VPHH

-----

-----L

CLM

STN

CMLD

PIIY

CFLT

KKGo

at-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

Y-N

LAGC

FFFI

NTY

CSVA

FLAV

I-----

----F

LLSL

IIWVS

IVGA

ASYF

FVL-

-IHIF

LVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFVI

CFVP

HH---

-----

VTLC

LLST

NCV

LDPI

IYCF

LT--

Guin

ea p

ig-F

PIVY

SIIF

VLGI

IAN

GYVL

W--

--FM

VNLT

VADL

LFLI

TLPL

WIV

--N

LAGC

LFFI

NTY

CSVA

FLGV

I-----

----I

ALSL

VIW

VAIV

AAAS

YFLV

M-

-IHIC

IVLG

FFIV

FLLI

LFCN

L--

RALW

MVC

TVLA

VFVI

CFVP

HH---

----Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Hedg

ehog

-FPI

FYSI

IFAL

GVIA

NSY

VLW

----F

MVN

LTLA

DLLF

LVTL

PLW

II--

NLA

GCFF

FIN

TYCS

VAFL

GVI--

-----

-GIIL

SLVI

WVA

IVSA

ASYF

FF--

-IHIF

LVVS

FFVV

FLIIF

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

---Q

ITLC

LLST

NCV

LDPI

IYCF

L---

Hors

e--P

IVYS

IIFVL

GVIA

NSY

VLW

V--IF

MVN

LTVA

DLLF

LLTL

PLW

V---

NLT

GCCF

FIN

TYCS

VAFL

GVI--

-----

--ISL

SLVI

WLA

IVAA

ASYF

LFM

--IH

IFLV

FSFF

LVFL

IILVC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

--QVT

LCLL

STN

CVLD

PIIY

CFL-

--M

edak

a-F

SVVY

SIFF

VVGL

LAN

VYVL

F--

--YM

TNLT

IADL

LFVC

ALPF

WIG

----T

GSFF

FIN

TYGT

ILFL

GAIS

L----

-RG

IIVSV

LIW

LCLV

SSAI

PSL-

----H

FLIIG

LFFV

VFFL

VMVC

NLL

----

QM

LLAV

VGVF

LLCF

LPHH

VVQ

----Q

ITLV

LMGL

NCI

LDPV

IYYF

A---

Mon

key

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

--N

LAGC

LFFI

NTY

CSVA

FLGV

I-----

----I

SLSL

VIW

VAIV

GAAS

YFFI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

--QVT

LCLL

STN

CVLD

PVIY

CFL-

--M

ouse

-FPI

VYSV

IFIL

GVVA

NGY

VLW

----F

MVN

LTM

ADLL

FLIT

LPLW

IV--

NVA

GCLF

FIN

TYCS

VAFL

GVI--

-----

-GIS

LSLI

IWVS

IVAT

ASYF

LA--

VVHV

FIAF

CFFL

VFFL

IFYC

N---

RALW

MVC

TVLA

VFIIC

FVPH

H----

----

ITLC

LLST

NCV

LDPV

IYCF

LT--

Opo

ssum

-FPI

VYSI

IFVL

GFVS

NAY

VLW

-----

MVN

LTVA

DLLF

LFAL

PLW

IVY-

NVA

GCLF

FIN

TYCS

VCFL

GVI--

-----

-GIL

LSTI

IWVV

IVGC

ASYF

LI--

--HVF

IVLC

FFIV

FLVI

LVCN

VI-

--LW

MVC

TVM

AVFF

ICFV

PHHV

V---

--QVT

LCLM

SIN

CVLD

PIIY

CFL-

--O

rang

utan

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

--N

VAGC

LFFI

NTY

CSVA

FLGV

I-----

----I

SLSL

VIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

---VT

LCLL

STN

CVLD

PVIY

CFLT

--Pi

g-F

PIFY

SIIF

VLGV

IAN

SYVL

W--

---M

LNLT

MAD

LLFL

VTLP

LWIIY

-N

LAGC

FFFI

NTY

CSVS

FLAV

I-----

----I

SLSL

IIWVA

MVA

AASY

FFVL

--IH

IFLV

FSFF

LVFL

VILF

CNL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

---IT

LCLL

STN

CVLD

PIIY

CFLT

--Pi

ka-F

PIVY

SIIF

VLGV

VAN

GYVL

W--

--FM

VNLT

VADL

LFLI

TLPL

WII-

-N

VAGC

LFFI

NTY

CSVA

FLGV

I-----

----I

SLSL

IIWVV

ILAI

ASYF

LIM

--IH

IFIV

FCFF

VVFL

IILVC

NL-

-RA

LWM

VCTV

LGVF

IICFV

PHH-

----

--QVT

LCLL

STN

CVLD

PVIY

CFL-

--Pl

atyp

us---

VAYS

IIFVL

GIFA

NGY

VLW

VF--F

MVN

LTVA

DLLF

LVTM

PLW

IV--

NVA

GCLF

FIN

TYCS

VAFL

GVI--

-----

-GIV

LSLV

IWVL

IVSS

ALYF

LV--

-IHIF

IVAS

FFAV

FLLI

LVCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

----

VTLC

LLST

NCV

LDPV

IYCF

LT--

Puffe

rfish

--PSA

YGVI

FILG

LLAN

LYVL

FI-

---M

TNLS

IADL

LFVC

ALPF

WIG

Y----

GSLF

FIN

TYCS

VLFL

GAIS

VN---

--G

IIISV

IIWVL

TISM

AIPY

LV--

---FA

IIGLF

FVVF

ALVL

VCN

LLI

-----L

LAVV

GVFV

VCFL

PHHV

IQGA

--QIT

LLLM

GLN

CILD

PVVY

CFA-

--Ra

bbit

--PIV

YSIIF

VLGV

VAN

GYVL

WV-

--FM

VNLT

VADL

LFLI

TLPL

WII-

-N

LAGC

LFFI

NTY

CSVA

FLGV

I-----

----I

ALSL

VIW

VSIS

AAAT

YFLI

L--IH

IFIV

ASFF

IVFL

IILIC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

--QVT

LCLL

STN

CVLD

PVIY

CFL-

--Ra

t--P

IVYS

VIFV

LGVV

ANGY

VLW

V---F

MVN

LTVA

DLLF

LMTL

PLW

IV--

NLA

GCLF

FIN

TYCS

VAFL

GVI--

-----

-GIT

LSLV

IWIS

IAAT

ASYF

LA--

-VHI

FITS

CFFL

VFFL

IFYC

NM

--RA

LWM

VCTV

LAVF

VICF

VPHH

-----

---IT

LCLL

STN

CVLD

PVIY

CFLT

--Sh

rew

-FPI

FYSI

IFVL

GVIA

NSY

VLW

----F

MVN

LTM

ADLL

FLIT

LPLW

IV--

NLT

GCFF

FIN

TYCS

VAFL

GVI--

-----

-GIY

LSLV

IWM

AIVS

AASY

FFF-

--IH

IFLV

CSFF

LVFL

LIFF

CNL-

-RA

LWM

VCTV

LAVF

SICF

VPHH

-----

---VT

LCLL

STN

CVLD

PIIY

CFLT

--Sq

uirr

elLF

PIIY

SIIF

VVGV

IAN

GYVL

-----F

MLN

LTVA

DLLF

LITL

PLW

II--

NVA

GCLF

FIN

TYCS

VAFL

GVI--

-----

---YL

SLVI

WVF

IVAA

ASYF

LILD

VIHI

FIVF

SFFL

VFLI

ILFC

K---

RALW

MVC

AVLA

VFVI

CFVP

HH---

----Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Stickleba

ck-F

PVVY

SIIF

ILGI

TAN

LYVL

F--

CIYM

INLT

IGDL

LFVT

FLPF

W---

---S

GSLF

FIN

TYSS

VLFL

CAIS

V----

--G

IILSV

FIW

LFTL

ATAI

LFLI

----H

LTIV

IGFF

LVFF

VVVV

CNFL

----

QM

LFSV

VGVF

VVCF

LPHH

ACQ

----Q

ITW

LLM

SLN

CILD

PVVY

FFA-

--Zebrafi

sh--P

IFYG

VVFV

LGLI

ANCY

ALYV

--IY

MTN

LTVA

DLLF

VSAL

PFW

I---

---GT

FFFI

NTY

GSIL

FLTV

ISIN

----

RGAI

ISVL

IWAF

ILSA

SIPQ

L---

ITHF

SIIG

LFFL

VFLM

VIIC

N---

--LRM

LCAV

IGVF

VICF

LPHH

VV---

--QIT

LMLM

GIN

CLLD

PLVY

CFA-

--


Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5108

61.9

4-F

KLVV

NCL

MLL

FLIL

IAVM

FH--

--HPR

FFFS

VALL

LLAV

FDIV

LR-

---HY

IAIV

ADTL

AVAY

VFTT

SVR

LESI

FLGL

TGLL

LSVN

LLSY

I----

-LGF

AMSV

APIF

FGFA

LFFC

IV--

MGF

SLVA

LYFV

MFG

DAIL

PAI--

---LI

VTVL

ANFM

TILF

ILFF

MM

T---

Hum

an-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

----

ISLS

LVIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Chic

ken

--TVF

YSIIF

ILGF

VAN

CYVL

WI-

--FM

LNLT

VADL

LFLV

TMPL

WIV

-SV

AGSL

FFVN

TYAS

VAFL

MVI

-----G

IFLS

AAIW

IITVG

SALY

YIF-

-AI

HIIIC

TLFY

VIFL

FILT

WN

---RA

LWM

VCTV

LVVF

IISFV

PHH-

----

-VTL

CLLS

MN

CVLD

PVIY

CFLT

--Ch

impa

nzee

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

---IS

LSLV

IWVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

--Co

w-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

FFFI

NTY

CSVA

FLAV

I---

---IL

LSLI

IWVS

IVGA

ASYF

FVL-

-IHIF

LVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PIIY

CFLT

--El

epha

nt-F

PIFY

SIIF

VLGV

IAN

GYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

LFFI

NTY

CSVA

FLGV

I---

---IT

LSLV

IWVT

IVAA

ASYF

LFM

---H

IFIV

CSFF

LVFF

IILVC

NLV

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--Fr

og---

VTYS

IIFVF

GFIA

NCY

VLW

IF-IF

MIN

LTIA

DLLF

LVTL

PLW

I--N

VAGC

FFFL

NTY

CSVA

FLGV

I---

----C

ISVA

VWIL

LFAS

SLYF

LIFP

--HFI

LVGA

FFVV

FLIIL

TCN

LV-

RALW

MVV

TVLA

VFVL

CFVP

HH---

-----

LCLM

STN

CMLD

PIIY

CFLT

KKGo

at-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

FFFI

NTY

CSVA

FLAV

I---

---FL

LSLI

IWVS

IVGA

ASYF

FVL-

-IHIF

LVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFVI

CFVP

HH---

---V

TLCL

LSTN

CVLD

PIIY

CFLT

--Gu

inea

pig

-FPI

VYSI

IFVL

GIIA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

----

IALS

LVIW

VAIV

AAAS

YFLV

M-

-IHIC

IVLG

FFIV

FLLI

LFCN

L--

RALW

MVC

TVLA

VFVI

CFVP

HH---

--Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Hedg

ehog

-FPI

FYSI

IFAL

GVIA

NSY

VLW

----F

MVN

LTLA

DLLF

LVTL

PLW

II-N

LAGC

FFFI

NTY

CSVA

FLGV

I---

--GIIL

SLVI

WVA

IVSA

ASYF

FF--

-IHIF

LVVS

FFVV

FLIIF

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

ITLC

LLST

NCV

LDPI

IYCF

L---

Hors

e--P

IVYS

IIFVL

GVIA

NSY

VLW

V--IF

MVN

LTVA

DLLF

LLTL

PLW

V--

NLT

GCCF

FIN

TYCS

VAFL

GVI--

----

ISLS

LVIW

LAIV

AAAS

YFLF

M-

-IHIF

LVFS

FFLV

FLIIL

VCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPI

IYCF

L---

Med

aka

-FSV

VYSI

FFVV

GLLA

NVY

VLF-

---Y

MTN

LTIA

DLLF

VCAL

PFW

IG-

--TGS

FFFI

NTY

GTIL

FLGA

ISL-

-RGI

IVSV

LIW

LCLV

SSAI

PSL-

----H

FLIIG

LFFV

VFFL

VMVC

NLL

----

QM

LLAV

VGVF

LLCF

LPHH

VVQ

--Q

ITLV

LMGL

NCI

LDPV

IYYF

A---

Mon

key

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

---IS

LSLV

IWVA

IVGA

ASYF

FIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Mou

se-F

PIVY

SVIF

ILGV

VAN

GYVL

W--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--GIS

LSLI

IWVS

IVAT

ASYF

LA--

VVHV

FIAF

CFFL

VFFL

IFYC

N---

RALW

MVC

TVLA

VFIIC

FVPH

H----

--IT

LCLL

STN

CVLD

PVIY

CFLT

--O

poss

um-F

PIVY

SIIF

VLGF

VSN

AYVL

W--

---M

VNLT

VADL

LFLF

ALPL

WIV

YN

VAGC

LFFI

NTY

CSVC

FLGV

I---

--HVF

IVLC

FFIV

FLVI

LVCN

VI-

--GIL

LSTI

IWVV

IVGC

ASYF

LI--

--LW

MVC

TVM

AVFF

ICFV

PHHV

V---

QVT

LCLM

SIN

CVLD

PIIY

CFL-

--O

rang

utan

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

---IS

LSLV

IWVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

--Pi

g-F

PIFY

SIIF

VLGV

IAN

SYVL

W--

---M

LNLT

MAD

LLFL

VTLP

LWIIY

NLA

GCFF

FIN

TYCS

VSFL

AVI--

----

ISLS

LIIW

VAM

VAAA

SYFF

VL-

-IHIF

LVFS

FFLV

FLVI

LFCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--IT

LCLL

STN

CVLD

PIIY

CFLT

--Pi

ka-F

PIVY

SIIF

VLGV

VAN

GYVL

W--

--FM

VNLT

VADL

LFLI

TLPL

WII-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

----

ISLS

LIIW

VVIL

AIAS

YFLI

M-

-IHIF

IVFC

FFVV

FLIIL

VCN

L--

RALW

MVC

TVLG

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Plat

ypus

---VA

YSIIF

VLGI

FAN

GYVL

WVF

--FM

VNLT

VADL

LFLV

TMPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--GIV

LSLV

IWVL

IVSS

ALYF

LV--

-IHIF

IVAS

FFAV

FLLI

LVCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

--Pu

fferfish

--PSA

YGVI

FILG

LLAN

LYVL

FI-

---M

TNLS

IADL

LFVC

ALPF

WIG

Y---

GSLF

FIN

TYCS

VLFL

GAIS

VN--G

IIISV

IIWVL

TISM

AIPY

LV--

---FA

IIGLF

FVVF

ALVL

VCN

LLI

-----L

LAVV

GVFV

VCFL

PHHV

IQGA

QIT

LLLM

GLN

CILD

PVVY

CFA-

--Ra

bbit

--PIV

YSIIF

VLGV

VAN

GYVL

WV-

--FM

VNLT

VADL

LFLI

TLPL

WII-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

----

IALS

LVIW

VSIS

AAAT

YFLI

L--IH

IFIV

ASFF

IVFL

IILIC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--Ra

t--P

IVYS

VIFV

LGVV

ANGY

VLW

V---F

MVN

LTVA

DLLF

LMTL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---G

ITLS

LVIW

ISIA

ATAS

YFLA

---V

HIFI

TSCF

FLVF

FLIF

YCN

M--

RALW

MVC

TVLA

VFVI

CFVP

HH---

---IT

LCLL

STN

CVLD

PVIY

CFLT

--Sh

rew

-FPI

FYSI

IFVL

GVIA

NSY

VLW

----F

MVN

LTM

ADLL

FLIT

LPLW

IV-

NLT

GCFF

FIN

TYCS

VAFL

GVI--

---G

IYLS

LVIW

MAI

VSAA

SYFF

F--

-IHIF

LVCS

FFLV

FLLI

FFCN

L--

RALW

MVC

TVLA

VFSI

CFVP

HH---

---V

TLCL

LSTN

CVLD

PIIY

CFLT

--Sq

uirr

elLF

PIIY

SIIF

VVGV

IAN

GYVL

-----F

MLN

LTVA

DLLF

LITL

PLW

II-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

----Y

LSLV

IWVF

IVAA

ASYF

LILD

VIHI

FIVF

SFFL

VFLI

ILFC

K---

RALW

MVC

AVLA

VFVI

CFVP

HH---

--Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Stickleba

ck-F

PVVY

SIIF

ILGI

TAN

LYVL

F--

CIYM

INLT

IGDL

LFVT

FLPF

W---

--SGS

LFFI

NTY

SSVL

FLCA

ISV-

--GIIL

SVFI

WLF

TLAT

AILF

LI--

---Q

MLF

SVVG

VFVV

CFLP

HHAC

Q--

--HLT

IVIG

FFLV

FFVV

VVCN

FL-

QIT

WLL

MSL

NCI

LDPV

VYFF

A---

Zebrafi

sh--P

IFYG

VVFV

LGLI

ANCY

ALYV

--IY

MTN

LTVA

DLLF

VSAL

PFW

I-----

GTFF

FIN

TYGS

ILFL

TVIS

IN-R

GAIIS

VLIW

AFIL

SASI

PQL-

--IT

HFSI

IGLF

FLVF

LMVI

ICN

-----L

RMLC

AVIG

VFVI

CFLP

HHVV

---Q

ITLM

LMGI

NCL

LDPL

VYCF

A---

Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5119

23.7

1-G

DV--A

IIMAA

PSLT

ALSV

AIRM

------

-GLA

ELLA

FALS

PAVI

IAHA

LR---

VFVL

LIPR

WPI

RMYL

SVAW

RVTL

FFCG

VAVA

IPSL

CTW

CSFL

---YL

FLLG

VLCV

WLS

PFSP

I-CLL

--GQ

PRLP

ICW

TLIL

CCAW

GMLS

------

------

VVVA

VAVF

LFLP

TGVR

CVL-

-----F

L---

Hum

an-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV---

--N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

-----R

ALW

--MVC

TVLA

VFIIC

FVPH

H----

----

-VTL

CLL-

--STN

CVLD

PVIY

CFLT

--Ch

icke

n--T

VFYS

IIFIL

GFVA

NCY

VLW

I---F

MLN

LTVA

DLLF

LVTM

PLW

IV---

--SV

AGSL

FFVN

TYAS

VAFL

MVI

----G

IFLS

AAIW

IITVG

SALY

YIF-

-AI

HIIIC

TLFY

VIFL

FILT

WN

------

--RAL

W--M

VCTV

LVVF

IISFV

PHH-

----

----V

TLCL

L---S

MN

CVLD

PVIY

CFLT

--Ch

impa

nzee

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-----

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

----

--RAL

W--M

VCTV

LAVF

IICFV

PHH-

----

----V

TLCL

L---S

TNCV

LDPV

IYCF

LT--

Cow

-FPI

FYSI

VFVL

GVIA

NSY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY-

---N

LAGC

FFFI

NTY

CSVA

FLAV

I---

--ILL

SLIIW

VSIV

GAAS

YFFV

L--IH

IFLV

FSFF

LVFL

IILFC

NL-

----

--RAL

W--M

VCTV

LAVF

IICFV

PHH-

----

----V

TLCL

L---S

TNCV

LDPI

IYCF

LT--

Elep

hant

-FPI

FYSI

IFVL

GVIA

NGY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY-

---N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--ITL

SLVI

WVT

IVAA

ASYF

LFM

---H

IFIV

CSFF

LVFF

IILVC

NLV

----

--RAL

W--M

VCTV

LAVF

IICFV

PHH-

----

---Q

VTLC

LL---

STN

CVLD

PVIY

CFL-

--Fr

og---

VTYS

IIFVF

GFIA

NCY

VLW

IF-IF

MIN

LTIA

DLLF

LVTL

PLW

I-----

-N

VAGC

FFFL

NTY

CSVA

FLGV

I---

---CI

SVAV

WIL

LFAS

SLYF

LIFP

--HFI

LVGA

FFVV

FLIIL

TCN

LV-

-----R

ALW

--MVV

TVLA

VFVL

CFVP

HH---

-----

---LC

LM---

STN

CMLD

PIIY

CFLT

KKGo

at-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

Y----

NLA

GCFF

FIN

TYCS

VAFL

AVI--

---F

LLSL

IIWVS

IVGA

ASYF

FVL-

-IHIF

LVFS

FFLV

FLIIL

FCN

L--

-----R

ALW

--MVC

TVLA

VFVI

CFVP

HH---

-----

-VTL

CLL-

--STN

CVLD

PIIY

CFLT

--Gu

inea

pig

-FPI

VYSI

IFVL

GIIA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

IV---

--N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--IAL

SLVI

WVA

IVAA

ASYF

LVM

--IH

ICIV

LGFF

IVFL

LILF

CNL-

----

--RAL

W--M

VCTV

LAVF

VICF

VPHH

-----

---Q

VTLC

LL---

STN

CVLD

PVIY

CFL-

--He

dgeh

og-F

PIFY

SIIF

ALGV

IAN

SYVL

W--

--FM

VNLT

LADL

LFLV

TLPL

WII-

----

NLA

GCFF

FIN

TYCS

VAFL

GVI--

--G

IILSL

VIW

VAIV

SAAS

YFFF

---IH

IFLV

VSFF

VVFL

IIFFC

NL-

----

--RAL

W--M

VCTV

LAVF

IICFV

PHH-

----

---Q

ITLC

LL---

STN

CVLD

PIIY

CFL-

--Ho

rse

--PIV

YSIIF

VLGV

IAN

SYVL

WV-

-IFM

VNLT

VADL

LFLL

TLPL

WV-

-----

NLT

GCCF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

LAIV

AAAS

YFLF

M-

-IHIF

LVFS

FFLV

FLIIL

VCN

L--

-----R

ALW

--MVC

TVLA

VFIIC

FVPH

H----

----

QVT

LCLL

---ST

NCV

LDPI

IYCF

L---

Med

aka

-FSV

VYSI

FFVV

GLLA

NVY

VLF-

---Y

MTN

LTIA

DLLF

VCAL

PFW

IG---

----T

GSFF

FIN

TYGT

ILFL

GAIS

L-RG

IIVSV

LIW

LCLV

SSAI

PSL-

----H

FLIIG

LFFV

VFFL

VMVC

NLL

----

-----Q

--MLL

AVVG

VFLL

CFLP

HHVV

Q--

---Q

ITLV

LM---

GLN

CILD

PVIY

YFA-

--M

onke

y-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV---

--N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

FIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

-----R

ALW

--MVC

TVLA

VFIIC

FVPH

H----

----

QVT

LCLL

---ST

NCV

LDPV

IYCF

L---

Mou

se-F

PIVY

SVIF

ILGV

VAN

GYVL

W--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

-----

NVA

GCLF

FIN

TYCS

VAFL

GVI--

--G

ISLS

LIIW

VSIV

ATAS

YFLA

--VV

HVFI

AFCF

FLVF

FLIF

YCN

------

--RAL

W--M

VCTV

LAVF

IICFV

PHH-

----

----IT

LCLL

---ST

NCV

LDPV

IYCF

LT--

Opo

ssum

-FPI

VYSI

IFVL

GFVS

NAY

VLW

-----

MVN

LTVA

DLLF

LFAL

PLW

IVY-

---N

VAGC

LFFI

NTY

CSVC

FLGV

I---

-GIL

LSTI

IWVV

IVGC

ASYF

LI--

--HVF

IVLC

FFIV

FLVI

LVCN

VI-

------

-LW

--MVC

TVM

AVFF

ICFV

PHHV

V---

---Q

VTLC

LM---

SIN

CVLD

PIIY

CFL-

--O

rang

utan

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-----

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

----

--RAL

W--M

VCTV

LAVF

IICFV

PHH-

----

----V

TLCL

L---S

TNCV

LDPV

IYCF

LT--

Pig

-FPI

FYSI

IFVL

GVIA

NSY

VLW

-----

MLN

LTM

ADLL

FLVT

LPLW

IIY---

-N

LAGC

FFFI

NTY

CSVS

FLAV

I---

--ISL

SLIIW

VAM

VAAA

SYFF

VL-

-IHIF

LVFS

FFLV

FLVI

LFCN

L--

-----R

ALW

--MVC

TVLA

VFIIC

FVPH

H----

----

-ITLC

LL---

STN

CVLD

PIIY

CFLT

--Pi

ka-F

PIVY

SIIF

VLGV

VAN

GYVL

W--

--FM

VNLT

VADL

LFLI

TLPL

WII-

----

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

IIWVV

ILAI

ASYF

LIM

--IH

IFIV

FCFF

VVFL

IILVC

NL-

----

--RAL

W--M

VCTV

LGVF

IICFV

PHH-

----

---Q

VTLC

LL---

STN

CVLD

PVIY

CFL-

--Pl

atyp

us---

VAYS

IIFVL

GIFA

NGY

VLW

VF--F

MVN

LTVA

DLLF

LVTM

PLW

IV---

--N

VAGC

LFFI

NTY

CSVA

FLGV

I---

-GIV

LSLV

IWVL

IVSS

ALYF

LV--

-IHIF

IVAS

FFAV

FLLI

LVCN

L--

-----R

ALW

--MVC

TVLA

VFIIC

FVPH

H----

----

-VTL

CLL-

--STN

CVLD

PVIY

CFLT

--Pu

fferfish

--PSA

YGVI

FILG

LLAN

LYVL

FI-

---M

TNLS

IADL

LFVC

ALPF

WIG

Y----

---GS

LFFI

NTY

CSVL

FLGA

ISVN

-GIII

SVIIW

VLTI

SMAI

PYLV

-----

FAIIG

LFFV

VFAL

VLVC

NLL

I---

------

---LL

AVVG

VFVV

CFLP

HHVI

QGA

---Q

ITLL

LM---

GLN

CILD

PVVY

CFA-

--Ra

bbit

--PIV

YSIIF

VLGV

VAN

GYVL

WV-

--FM

VNLT

VADL

LFLI

TLPL

WII-

----

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

ALSL

VIW

VSIS

AAAT

YFLI

L--IH

IFIV

ASFF

IVFL

IILIC

NL-

----

--RAL

W--M

VCTV

LAVF

IICFV

PHH-

----

---Q

VTLC

LL---

STN

CVLD

PVIY

CFL-

--Ra

t--P

IVYS

VIFV

LGVV

ANGY

VLW

V---F

MVN

LTVA

DLLF

LMTL

PLW

IV---

--N

LAGC

LFFI

NTY

CSVA

FLGV

I---

-GIT

LSLV

IWIS

IAAT

ASYF

LA--

-VHI

FITS

CFFL

VFFL

IFYC

NM

-----

--RAL

W--M

VCTV

LAVF

VICF

VPHH

-----

----IT

LCLL

---ST

NCV

LDPV

IYCF

LT--

Shre

w-F

PIFY

SIIF

VLGV

IAN

SYVL

W--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

-----

NLT

GCFF

FIN

TYCS

VAFL

GVI--

--G

IYLS

LVIW

MAI

VSAA

SYFF

F--

-IHIF

LVCS

FFLV

FLLI

FFCN

L--

-----R

ALW

--MVC

TVLA

VFSI

CFVP

HH---

-----

-VTL

CLL-

--STN

CVLD

PIIY

CFLT

--Sq

uirr

elLF

PIIY

SIIF

VVGV

IAN

GYVL

-----F

MLN

LTVA

DLLF

LITL

PLW

II----

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

---YL

SLVI

WVF

IVAA

ASYF

LILD

VIHI

FIVF

SFFL

VFLI

ILFC

K---

-----R

ALW

--MVC

AVLA

VFVI

CFVP

HH---

-----

QVT

LCLL

---ST

NCV

LDPV

IYCF

L---

Stickleba

ck-F

PVVY

SIIF

ILGI

TAN

LYVL

F--

CIYM

INLT

IGDL

LFVT

FLPF

W---

----

--SGS

LFFI

NTY

SSVL

FLCA

ISV-

-GIIL

SVFI

WLF

TLAT

AILF

LI--

--HLT

IVIG

FFLV

FFVV

VVCN

FL-

------

--Q--M

LFSV

VGVF

VVCF

LPHH

ACQ

-----

QIT

WLL

M---

SLN

CILD

PVVY

FFA-

--Zebrafi

sh--P

IFYG

VVFV

LGLI

ANCY

ALYV

--IY

MTN

LTVA

DLLF

VSAL

PFW

I-----

----

GTFF

FIN

TYGS

ILFL

TVIS

INRG

AIIS

VLIW

AFIL

SASI

PQL-

--IT

HFSI

IGLF

FLVF

LMVI

ICN

------

----L

R--M

LCAV

IGVF

VICF

LPHH

VV---

---Q

ITLM

LM---

GIN

CLLD

PLVY

CFA-

--


Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5119

21.7

1TL

FFCG

VAVA

-ILSL

CT---

WCG

FL-V

SGRR

SLIC

FTCG

M--V

ETLP

AT-A

MRC

CSGV

FCFL

EAFI

SFPS

C--

-SFT

LCVG

SVTL

SCVI

HAVC

TF--

VAVP

RLLW

TGCL

TAVF

MEL

LW---

------

CGSL

LLLF

YGGC

SV-V

VCAC

FL---

-GDV

--AIIM

AAPS

LTAL

SVAI

RMHu

man

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

--N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

----R

ALW

MVC

TVLA

VFIIC

FVPH

H----

---V

TLCL

LSTN

CVLD

PVIY

CFLT

--Ch

icke

n--T

VFYS

IIFIL

GFVA

NCY

VLW

I---F

MLN

LTVA

DLLF

LVTM

PLW

IV--

SVAG

SLFF

VNTY

ASVA

FLM

VI---

-GIF

LSAA

IWIIT

VGSA

LYYI

F--

AIHI

IICTL

FYVI

FLFI

LTW

N---

----R

ALW

MVC

TVLV

VFIIS

FVPH

H----

---V

TLCL

LSM

NCV

LDPV

IYCF

LT--

Chim

panz

ee-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV--

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

----

-RAL

WM

VCTV

LAVF

IICFV

PHH-

----

--VTL

CLLS

TNCV

LDPV

IYCF

LT--

Cow

-FPI

FYSI

VFVL

GVIA

NSY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY-

NLA

GCFF

FIN

TYCS

VAFL

AVI--

---I

LLSL

IIWVS

IVGA

ASYF

FVL-

-IHIF

LVFS

FFLV

FLIIL

FCN

L--

----R

ALW

MVC

TVLA

VFIIC

FVPH

H----

---V

TLCL

LSTN

CVLD

PIIY

CFLT

--El

epha

nt-F

PIFY

SIIF

VLGV

IAN

GYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

Y-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--ITL

SLVI

WVT

IVAA

ASYF

LFM

---H

IFIV

CSFF

LVFF

IILVC

NLV

----

-RAL

WM

VCTV

LAVF

IICFV

PHH-

----

-QVT

LCLL

STN

CVLD

PVIY

CFL-

--Fr

og---

VTYS

IIFVF

GFIA

NCY

VLW

IF-IF

MIN

LTIA

DLLF

LVTL

PLW

I---

NVA

GCFF

FLN

TYCS

VAFL

GVI--

----

CISV

AVW

ILLF

ASSL

YFLI

FP--H

FILV

GAFF

VVFL

IILTC

NLV

----

-RAL

WM

VVTV

LAVF

VLCF

VPHH

-----

----L

CLM

STN

CMLD

PIIY

CFLT

KKGo

at-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

Y-N

LAGC

FFFI

NTY

CSVA

FLAV

I---

--FLL

SLIIW

VSIV

GAAS

YFFV

L--IH

IFLV

FSFF

LVFL

IILFC

NL-

----

-RAL

WM

VCTV

LAVF

VICF

VPHH

-----

--VTL

CLLS

TNCV

LDPI

IYCF

LT--

Guin

ea p

ig-F

PIVY

SIIF

VLGI

IAN

GYVL

W--

--FM

VNLT

VADL

LFLI

TLPL

WIV

--N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--IAL

SLVI

WVA

IVAA

ASYF

LVM

--IH

ICIV

LGFF

IVFL

LILF

CNL-

----

-RAL

WM

VCTV

LAVF

VICF

VPHH

-----

-QVT

LCLL

STN

CVLD

PVIY

CFL-

--He

dgeh

og-F

PIFY

SIIF

ALGV

IAN

SYVL

W--

--FM

VNLT

LADL

LFLV

TLPL

WII-

-N

LAGC

FFFI

NTY

CSVA

FLGV

I---

-GIIL

SLVI

WVA

IVSA

ASYF

FF--

-IHIF

LVVS

FFVV

FLIIF

FCN

L--

----R

ALW

MVC

TVLA

VFIIC

FVPH

H----

--Q

ITLC

LLST

NCV

LDPI

IYCF

L---

Hors

e--P

IVYS

IIFVL

GVIA

NSY

VLW

V--IF

MVN

LTVA

DLLF

LLTL

PLW

V---

NLT

GCCF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

LAIV

AAAS

YFLF

M-

-IHIF

LVFS

FFLV

FLIIL

VCN

L--

----R

ALW

MVC

TVLA

VFIIC

FVPH

H----

--Q

VTLC

LLST

NCV

LDPI

IYCF

L---

Med

aka

-FSV

VYSI

FFVV

GLLA

NVY

VLF-

---Y

MTN

LTIA

DLLF

VCAL

PFW

IG--

--TGS

FFFI

NTY

GTIL

FLGA

ISL-

RGIIV

SVLI

WLC

LVSS

AIPS

L---

--HFL

IIGLF

FVVF

FLVM

VCN

LL-

------

-QM

LLAV

VGVF

LLCF

LPHH

VVQ

---Q

ITLV

LMGL

NCI

LDPV

IYYF

A---

Mon

key

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

--N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

FIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

----R

ALW

MVC

TVLA

VFIIC

FVPH

H----

--Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Mou

se-F

PIVY

SVIF

ILGV

VAN

GYVL

W--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

--N

VAGC

LFFI

NTY

CSVA

FLGV

I---

-GIS

LSLI

IWVS

IVAT

ASYF

LA--

VVHV

FIAF

CFFL

VFFL

IFYC

N---

----R

ALW

MVC

TVLA

VFIIC

FVPH

H----

---I

TLCL

LSTN

CVLD

PVIY

CFLT

--O

poss

um-F

PIVY

SIIF

VLGF

VSN

AYVL

W--

---M

VNLT

VADL

LFLF

ALPL

WIV

Y-N

VAGC

LFFI

NTY

CSVC

FLGV

I---

-GIL

LSTI

IWVV

IVGC

ASYF

LI--

--HVF

IVLC

FFIV

FLVI

LVCN

VI-

------

LWM

VCTV

MAV

FFIC

FVPH

HVV-

---Q

VTLC

LMSI

NCV

LDPI

IYCF

L---

Ora

ngut

an-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV--

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

----

-RAL

WM

VCTV

LAVF

IICFV

PHH-

----

--VTL

CLLS

TNCV

LDPV

IYCF

LT--

Pig

-FPI

FYSI

IFVL

GVIA

NSY

VLW

-----

MLN

LTM

ADLL

FLVT

LPLW

IIY-

NLA

GCFF

FIN

TYCS

VSFL

AVI--

---I

SLSL

IIWVA

MVA

AASY

FFVL

--IH

IFLV

FSFF

LVFL

VILF

CNL-

----

-RAL

WM

VCTV

LAVF

IICFV

PHH-

----

--ITL

CLLS

TNCV

LDPI

IYCF

LT--

Pika

-FPI

VYSI

IFVL

GVVA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

II--

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

IIWVV

ILAI

ASYF

LIM

--IH

IFIV

FCFF

VVFL

IILVC

NL-

----

-RAL

WM

VCTV

LGVF

IICFV

PHH-

----

-QVT

LCLL

STN

CVLD

PVIY

CFL-

--Pl

atyp

us---

VAYS

IIFVL

GIFA

NGY

VLW

VF--F

MVN

LTVA

DLLF

LVTM

PLW

IV--

NVA

GCLF

FIN

TYCS

VAFL

GVI--

--G

IVLS

LVIW

VLIV

SSAL

YFLV

---IH

IFIV

ASFF

AVFL

LILV

CNL-

----

-RAL

WM

VCTV

LAVF

IICFV

PHH-

----

--VTL

CLLS

TNCV

LDPV

IYCF

LT--

Puffe

rfish

--PSA

YGVI

FILG

LLAN

LYVL

FI-

---M

TNLS

IADL

LFVC

ALPF

WIG

Y----

GSLF

FIN

TYCS

VLFL

GAIS

VN-G

IIISV

IIWVL

TISM

AIPY

LV--

---FA

IIGLF

FVVF

ALVL

VCN

LLI

------

---LL

AVVG

VFVV

CFLP

HHVI

QGA

-QIT

LLLM

GLN

CILD

PVVY

CFA-

--Ra

bbit

--PIV

YSIIF

VLGV

VAN

GYVL

WV-

--FM

VNLT

VADL

LFLI

TLPL

WII-

-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--IAL

SLVI

WVS

ISAA

ATYF

LIL-

-IHIF

IVAS

FFIV

FLIIL

ICN

L--

----R

ALW

MVC

TVLA

VFIIC

FVPH

H----

--Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Rat

--PIV

YSVI

FVLG

VVAN

GYVL

WV-

--FM

VNLT

VADL

LFLM

TLPL

WIV

--N

LAGC

LFFI

NTY

CSVA

FLGV

I---

-GIT

LSLV

IWIS

IAAT

ASYF

LA--

-VHI

FITS

CFFL

VFFL

IFYC

NM

-----

-RAL

WM

VCTV

LAVF

VICF

VPHH

-----

--ITL

CLLS

TNCV

LDPV

IYCF

LT--

Shre

w-F

PIFY

SIIF

VLGV

IAN

SYVL

W--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

--N

LTGC

FFFI

NTY

CSVA

FLGV

I---

-GIY

LSLV

IWM

AIVS

AASY

FFF-

--IH

IFLV

CSFF

LVFL

LIFF

CNL-

----

-RAL

WM

VCTV

LAVF

SICF

VPHH

-----

--VTL

CLLS

TNCV

LDPI

IYCF

LT--

Squi

rrel

LFPI

IYSI

IFVV

GVIA

NGY

VL---

--FM

LNLT

VADL

LFLI

TLPL

WII-

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

---YL

SLVI

WVF

IVAA

ASYF

LILD

VIHI

FIVF

SFFL

VFLI

ILFC

K---

----R

ALW

MVC

AVLA

VFVI

CFVP

HH---

---Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Stickleba

ck-F

PVVY

SIIF

ILGI

TAN

LYVL

F--

CIYM

INLT

IGDL

LFVT

FLPF

W---

---S

GSLF

FIN

TYSS

VLFL

CAIS

V--G

IILSV

FIW

LFTL

ATAI

LFLI

----H

LTIV

IGFF

LVFF

VVVV

CNFL

----

----Q

MLF

SVVG

VFVV

CFLP

HHAC

Q--

-QIT

WLL

MSL

NCI

LDPV

VYFF

A---

Zebrafi

sh--P

IFYG

VVFV

LGLI

ANCY

ALYV

--IY

MTN

LTVA

DLLF

VSAL

PFW

I---

---GT

FFFI

NTY

GSIL

FLTV

ISIN

RGAI

ISVL

IWAF

ILSA

SIPQ

L---

ITHF

SIIG

LFFL

VFLM

VIIC

N---

------

LRM

LCAV

IGVF

VICF

LPHH

VV---

-QIT

LMLM

GIN

CLLD

PLVY

CFA-

--

Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5037

35.3

0-F

KLVV

NCL

MLL

FLIL

IAVM

FH--

PRFF

FSVA

---LL

LLAV

FDIV

LRY

----Y

IAIV

ADTL

GVAY

VFTT

SVRL

LESI

FLGL

TGLL

LSVN

LLSY

I----

-LGF

AMSV

APIF

FGFA

LFFC

IV--

MGF

SLVA

LYFV

MFG

DAIL

PAI--

---LI

VTVL

ANFM

TMLF

ILFF

MM

T---

Hum

an-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

-----

ISLS

LVIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Chic

ken

--TVF

YSIIF

ILGF

VAN

CYVL

WI-

--FM

LNLT

VADL

LFLV

TMPL

WIV

-SV

AGSL

FFVN

TYAS

VAFL

MVI

----

--GIF

LSAA

IWIIT

VGSA

LYYI

F--

AIHI

IICTL

FYVI

FLFI

LTW

N---

RALW

MVC

TVLV

VFIIS

FVPH

H----

--V

TLCL

LSM

NCV

LDPV

IYCF

LT--

Chim

panz

ee-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

-----

ISLS

LVIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Cow

-FPI

FYSI

VFVL

GVIA

NSY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCFF

FIN

TYCS

VAFL

AVI--

-----

ILLS

LIIW

VSIV

GAAS

YFFV

L--IH

IFLV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPI

IYCF

LT--

Elep

hant

-FPI

FYSI

IFVL

GVIA

NGY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCLF

FIN

TYCS

VAFL

GVI--

-----

ITLS

LVIW

VTIV

AAAS

YFLF

M-

--HIF

IVCS

FFLV

FFIIL

VCN

LV-

RALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Frog

---VT

YSIIF

VFGF

IAN

CYVL

WIF

-IFM

INLT

IADL

LFLV

TLPL

WI--

NVA

GCFF

FLN

TYCS

VAFL

GVI--

-----

-CIS

VAVW

ILLF

ASSL

YFLI

FP--H

FILV

GAFF

VVFL

IILTC

NLV

-RA

LWM

VVTV

LAVF

VLCF

VPHH

-----

---LC

LMST

NCM

LDPI

IYCF

LTKK

Goat

-FPI

FYSI

VFVL

GVIA

NSY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCFF

FIN

TYCS

VAFL

AVI--

-----

FLLS

LIIW

VSIV

GAAS

YFFV

L--IH

IFLV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

VICF

VPHH

-----

-VTL

CLLS

TNCV

LDPI

IYCF

LT--

Guin

ea p

ig-F

PIVY

SIIF

VLGI

IAN

GYVL

W--

--FM

VNLT

VADL

LFLI

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

I----

---IA

LSLV

IWVA

IVAA

ASYF

LVM

--IH

ICIV

LGFF

IVFL

LILF

CNL-

-RA

LWM

VCTV

LAVF

VICF

VPHH

-----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--He

dgeh

og-F

PIFY

SIIF

ALGV

IAN

SYVL

W--

--FM

VNLT

LADL

LFLV

TLPL

WII-

NLA

GCFF

FIN

TYCS

VAFL

GVI--

----G

IILSL

VIW

VAIV

SAAS

YFFF

---IH

IFLV

VSFF

VVFL

IIFFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

QIT

LCLL

STN

CVLD

PIIY

CFL-

--Ho

rse

--PIV

YSIIF

VLGV

IAN

SYVL

WV-

NLT

GCCF

FIN

TYCS

VAFL

GVI--

---IF

MVN

LTVA

DLLF

LLTL

PLW

V--

---IS

LSLV

IWLA

IVAA

ASYF

LFM

--IH

IFLV

FSFF

LVFL

IILVC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PIIY

CFL-

--M

edak

a-F

SVVY

SIFF

VVGL

LAN

VYVL

F--

--YM

TNLT

IADL

LFVC

ALPF

WIG

---T

GSFF

FIN

TYGT

ILFL

GAIS

L--

-RGI

IVSV

LIW

LCLV

SSAI

PSL-

----H

FLIIG

LFFV

VFFL

VMVC

NLL

----

QM

LLAV

VGVF

LLCF

LPHH

VVQ

--Q

ITLV

LMGL

NCI

LDPV

IYYF

A---

Mon

key

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

I----

---IS

LSLV

IWVA

IVGA

ASYF

FIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Mou

se-F

PIVY

SVIF

ILGV

VAN

GYVL

W--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I----

--GIS

LSLI

IWVS

IVAT

ASYF

LA--

VVHV

FIAF

CFFL

VFFL

IFYC

N---

RALW

MVC

TVLA

VFIIC

FVPH

H----

--IT

LCLL

STN

CVLD

PVIY

CFLT

--O

poss

um-F

PIVY

SIIF

VLGF

VSN

AYVL

W--

---M

VNLT

VADL

LFLF

ALPL

WIV

YN

VAGC

LFFI

NTY

CSVC

FLGV

I----

--GIL

LSTI

IWVV

IVGC

ASYF

LI--

--HVF

IVLC

FFIV

FLVI

LVCN

VI-

--LW

MVC

TVM

AVFF

ICFV

PHHV

V---

QVT

LCLM

SIN

CVLD

PIIY

CFL-

--O

rang

utan

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I----

---IS

LSLV

IWVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

--Pi

g-F

PIFY

SIIF

VLGV

IAN

SYVL

W--

---M

LNLT

MAD

LLFL

VTLP

LWIIY

NLA

GCFF

FIN

TYCS

VSFL

AVI--

-----

ISLS

LIIW

VAM

VAAA

SYFF

VL-

-IHIF

LVFS

FFLV

FLVI

LFCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--IT

LCLL

STN

CVLD

PIIY

CFLT

--Pi

ka-F

PIVY

SIIF

VLGV

VAN

GYVL

W--

--FM

VNLT

VADL

LFLI

TLPL

WII-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

-----

ISLS

LIIW

VVIL

AIAS

YFLI

M-

-IHIF

IVFC

FFVV

FLIIL

VCN

L--

QVT

LCLL

STN

CVLD

PVIY

CFL-

--RA

LWM

VCTV

LGVF

IICFV

PHH-

----

Plat

ypus

---VA

YSIIF

VLGI

FAN

GYVL

WVF

--FM

VNLT

VADL

LFLV

TMPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I----

-IHIF

IVAS

FFAV

FLLI

LVCN

L--

--GIV

LSLV

IWVL

IVSS

ALYF

LV--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

--Pu

fferfish

--PSA

YGVI

FILG

LLAN

LYVL

FI-

---M

TNLS

IADL

LFVC

ALPF

WIG

Y---

GSLF

FIN

TYCS

VLFL

GAIS

VN-

--GIII

SVIIW

VLTI

SMAI

PYLV

-----

FAIIG

LFFV

VFAL

VLVC

NLL

I---

--LLA

VVGV

FVVC

FLPH

HVIQ

GAQ

ITLL

LMGL

NCI

LDPV

VYCF

A---

Rabb

it--P

IVYS

IIFVL

GVVA

NGY

VLW

V---F

MVN

LTVA

DLLF

LITL

PLW

II-N

LAGC

LFFI

NTY

CSVA

FLGV

I----

---IA

LSLV

IWVS

ISAA

ATYF

LIL-

-IHIF

IVAS

FFIV

FLIIL

ICN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Rat

--PIV

YSVI

FVLG

VVAN

GYVL

WV-

--FM

VNLT

VADL

LFLM

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

I----

--GIT

LSLV

IWIS

IAAT

ASYF

LA--

RALW

MVC

TVLA

VFVI

CFVP

HH---

---V

HIFI

TSCF

FLVF

FLIF

YCN

M--

-ITLC

LLST

NCV

LDPV

IYCF

LT--

Shre

w-F

PIFY

SIIF

VLGV

IAN

SYVL

W--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

-N

LTGC

FFFI

NTY

CSVA

FLGV

I----

--GIY

LSLV

IWM

AIVS

AASY

FFF-

--IH

IFLV

CSFF

LVFL

LIFF

CNL-

-RA

LWM

VCTV

LAVF

SICF

VPHH

-----

-VTL

CLLS

TNCV

LDPI

IYCF

LT--

Squi

rrel

LFPI

IYSI

IFVV

GVIA

NGY

VL---

--FM

LNLT

VADL

LFLI

TLPL

WII-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

-----

-YLS

LVIW

VFIV

AAAS

YFLI

LDVI

HIFI

VFSF

FLVF

LIIL

FCK-

--RA

LWM

VCAV

LAVF

VICF

VPHH

-----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--Sti

ckleba

ck-F

PVVY

SIIF

ILGI

TAN

LYVL

F--

CIYM

INLT

IGDL

LFVT

FLPF

W---

--SGS

LFFI

NTY

SSVL

FLCA

ISV-

---G

IILSV

FIW

LFTL

ATAI

LFLI

----H

LTIV

IGFF

LVFF

VVVV

CNFL

----

QM

LFSV

VGVF

VVCF

LPHH

ACQ

--Q

ITW

LLM

SLN

CILD

PVVY

FFA-

--Zebrafi

sh-IY

MTN

LTVA

DLLF

VSAL

PFW

I----P

IFYG

VVFV

LGLI

ANCY

ALYV

----

GTFF

FIN

TYGS

ILFL

TVIS

IN-

-RGA

IISVL

IWAF

ILSA

SIPQ

L---

--LRM

LCAV

IGVF

VICF

LPHH

VV---

ITHF

SIIG

LFFL

VFLM

VIIC

N---

QIT

LMLM

GIN

CLLD

PLVY

CFA-

--


Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5103

55.1

90-L

EMVF

FIN

IVGC

LVPG

GACL

L--

KLFF

VVN

FVN

VLAT

YLM

FSSF

----

------

VSSS

TWVS

VFFV

CIGA

SLTS

G-S

LGAA

VIM

FTYI

LVFA

SNAA

L--

CLVH

FFVL

MLS

SMTL

LGPV

VA---

----

--QIV

VVGV

SYGV

YQVF

NGV

VLF

DSVL

QLL

LGLI

LLFV

GSYC

YV---

Hum

an-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

---FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GV---

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

--IHI

FIVF

SFFL

VFLI

ILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Chic

ken

--TVF

YSIIF

ILGF

VAN

CYVL

WI-

---FM

LNLT

VADL

LFLV

TMPL

WIV

-SV

AGSL

FFVN

TYAS

VAFL

MV-

--I---

-GIF

LSAA

IWIIT

VGSA

LYYI

F--

-AIH

IIICT

LFYV

IFLF

ILTW

N---

RALW

MVC

TVLV

VFIIS

FVPH

H----

--V

TLCL

LSM

NCV

LDPV

IYCF

LT--

Chim

panz

ee-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

---FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GV---

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

--IHI

FIVF

SFFL

VFLI

ILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Cow

-FPI

FYSI

VFVL

GVIA

NSY

VLW

-----

-MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCFF

FIN

TYCS

VAFL

AV---

I---

--ILL

SLIIW

VSIV

GAAS

YFFV

L---I

HIFL

VFSF

FLVF

LIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PIIY

CFLT

--El

epha

nt-F

PIFY

SIIF

VLGV

IAN

GYVL

W--

----M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

LFFI

NTY

CSVA

FLGV

---I--

---I

TLSL

VIW

VTIV

AAAS

YFLF

M-

---HI

FIVC

SFFL

VFFI

ILVCN

LV-

RALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Frog

---VT

YSIIF

VFGF

IAN

CYVL

WIF

--IFM

INLT

IADL

LFLV

TLPL

WI--

NVA

GCFF

FLN

TYCS

VAFL

GV---

I---

---CI

SVAV

WIL

LFAS

SLYF

LIFP

---HF

ILVGA

FFVV

FLIIL

TCN

LV-

RALW

MVV

TVLA

VFVL

CFVP

HH---

-----

LCLM

STN

CMLD

PIIY

CFLT

KKGo

at-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

----M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

FFFI

NTY

CSVA

FLAV

---I--

---F

LLSL

IIWVS

IVGA

ASYF

FVL-

--IHI

FLVF

SFFL

VFLI

ILFC

NL-

-RA

LWM

VCTV

LAVF

VICF

VPHH

-----

-VTL

CLLS

TNCV

LDPI

IYCF

LT--

Guin

ea p

ig-F

PIVY

SIIF

VLGI

IAN

GYVL

W--

---FM

VNLT

VADL

LFLI

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

---I--

---I

ALSL

VIW

VAIV

AAAS

YFLV

M-

--IHI

CIVL

GFFI

VFLL

ILFC

NL-

-RA

LWM

VCTV

LAVF

VICF

VPHH

-----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--He

dgeh

og-F

PIFY

SIIF

ALGV

IAN

SYVL

W--

---FM

VNLT

LADL

LFLV

TLPL

WII-

NLA

GCFF

FIN

TYCS

VAFL

GV---

I---

-GIIL

SLVI

WVA

IVSA

ASYF

FF--

--IHI

FLVV

SFFV

VFLI

IFFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

QIT

LCLL

STN

CVLD

PIIY

CFL-

--Ho

rse

--PIV

YSIIF

VLGV

IAN

SYVL

WV-

--IFM

VNLT

VADL

LFLL

TLPL

WV-

-N

LTGC

CFFI

NTY

CSVA

FLGV

---I--

---I

SLSL

VIW

LAIV

AAAS

YFLF

M-

--IHI

FLVF

SFFL

VFLI

ILVCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPI

IYCF

L---

Med

aka

-FSV

VYSI

FFVV

GLLA

NVY

VLF-

----

YMTN

LTIA

DLLF

VCAL

PFW

IG-

--TGS

FFFI

NTY

GTIL

FLGA

---IS

L-RG

IIVSV

LIW

LCLV

SSAI

PSL-

-----

HFLI

IGLF

FVVF

FLVM

VCN

LL-

---Q

MLL

AVVG

VFLL

CFLP

HHVV

Q--

QIT

LVLM

GLN

CILD

PVIY

YFA-

--M

onke

y-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

---FM

VNLT

MAD

MLF

LITL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GV---

I---

--ISL

SLVI

WVA

IVGA

ASYF

FIL-

--IHI

FIVF

SFFL

VFLI

ILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--M

ouse

-FPI

VYSV

IFIL

GVVA

NGY

VLW

-----

FMVN

LTM

ADLL

FLIT

LPLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GV---

I---

-GIS

LSLI

IWVS

IVAT

ASYF

LA--

-VVH

VFIA

FCFF

LVFF

LIFY

CN---

RALW

MVC

TVLA

VFIIC

FVPH

H----

--IT

LCLL

STN

CVLD

PVIY

CFLT

--O

poss

um-F

PIVY

SIIF

VLGF

VSN

AYVL

W--

----M

VNLT

VADL

LFLF

ALPL

WIV

YN

VAGC

LFFI

NTY

CSVC

FLGV

---I--

--G

ILLS

TIIW

VVIV

GCAS

YFLI

-----

HVFI

VLCF

FIVF

LVILV

CNVI

---L

WM

VCTV

MAV

FFIC

FVPH

HVV-

--Q

VTLC

LMSI

NCV

LDPI

IYCF

L---

Ora

ngut

an-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

---FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GV---

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

--IHI

FIVF

SFFL

VFLI

ILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Pig

-FPI

FYSI

IFVL

GVIA

NSY

VLW

-----

-MLN

LTM

ADLL

FLVT

LPLW

IIYN

LAGC

FFFI

NTY

CSVS

FLAV

---I--

---I

SLSL

IIWVA

MVA

AASY

FFVL

---I

HIFL

VFSF

FLVF

LVIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--IT

LCLL

STN

CVLD

PIIY

CFLT

--Pi

ka-F

PIVY

SIIF

VLGV

VAN

GYVL

W--

---FM

VNLT

VADL

LFLI

TLPL

WII-

NVA

GCLF

FIN

TYCS

VAFL

GV---

I---

--ISL

SLIIW

VVIL

AIAS

YFLI

M-

--IHI

FIVF

CFFV

VFLI

ILVCN

L--

RALW

MVC

TVLG

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Plat

ypus

---VA

YSIIF

VLGI

FAN

GYVL

WVF

---FM

VNLT

VADL

LFLV

TMPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

---I--

--G

IVLS

LVIW

VLIV

SSAL

YFLV

----I

HIFI

VASF

FAVF

LLILV

CNL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Puffe

rfish

--PSA

YGVI

FILG

LLAN

LYVL

FI-

----M

TNLS

IADL

LFVC

ALPF

WIG

Y---

GSLF

FIN

TYCS

VLFL

GA---

ISVN

-GIII

SVIIW

VLTI

SMAI

PYLV

-----

-FAI

IGLF

FVVF

ALVL

VCN

LLI

-----L

LAVV

GVFV

VCFL

PHHV

IQGA

QIT

LLLM

GLN

CILD

PVVY

CFA-

--Ra

bbit

--PIV

YSIIF

VLGV

VAN

GYVL

WV-

---FM

VNLT

VADL

LFLI

TLPL

WII-

NLA

GCLF

FIN

TYCS

VAFL

GV---

I---

--IAL

SLVI

WVS

ISAA

ATYF

LIL-

--IHI

FIVA

SFFI

VFLI

ILIC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--Ra

t--P

IVYS

VIFV

LGVV

ANGY

VLW

V----

FMVN

LTVA

DLLF

LMTL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GV---

I---

-GIT

LSLV

IWIS

IAAT

ASYF

LA--

--VHI

FITS

CFFL

VFFL

IFYC

NM

--RA

LWM

VCTV

LAVF

VICF

VPHH

-----

-ITLC

LLST

NCV

LDPV

IYCF

LT--

Shre

w-F

PIFY

SIIF

VLGV

IAN

SYVL

W--

---FM

VNLT

MAD

LLFL

ITLP

LWIV

-N

LTGC

FFFI

NTY

CSVA

FLGV

---I--

--G

IYLS

LVIW

MAI

VSAA

SYFF

F--

--IHI

FLVC

SFFL

VFLL

IFFC

NL-

-RA

LWM

VCTV

LAVF

SICF

VPHH

-----

-VTL

CLLS

TNCV

LDPI

IYCF

LT--

Squi

rrel

LFPI

IYSI

IFVV

GVIA

NGY

VL---

---FM

LNLT

VADL

LFLI

TLPL

WII-

NVA

GCLF

FIN

TYCS

VAFL

GV---

I---

---YL

SLVI

WVF

IVAA

ASYF

LILD

-VIH

IFIV

FSFF

LVFL

IILFC

K---

RALW

MVC

AVLA

VFVI

CFVP

HH---

--Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Stickleba

ck-F

PVVY

SIIF

ILGI

TAN

LYVL

F--

-CIY

MIN

LTIG

DLLF

VTFL

PFW

-----S

GSLF

FIN

TYSS

VLFL

CA---

ISV-

-GIIL

SVFI

WLF

TLAT

AILF

LI--

---HL

TIVI

GFFL

VFFV

VVVC

NFL

----

QM

LFSV

VGVF

VVCF

LPHH

ACQ

--Q

ITW

LLM

SLN

CILD

PVVY

FFA-

--Zebrafi

sh--P

IFYG

VVFV

LGLI

ANCY

ALYV

---I

YMTN

LTVA

DLLF

VSAL

PFW

I-----

GTFF

FIN

TYGS

ILFL

TV---

ISIN

RGAI

ISVL

IWAF

ILSA

SIPQ

L---

-ITHF

SIIG

LFFL

VFLM

VIIC

N---

--LRM

LCAV

IGVF

VICF

LPHH

VV---

QIT

LMLM

GIN

CLLD

PLVY

CFA-

--

Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5097

19.5

6-L

SFSL

SLSL

FFFF

LHVC

TLPL

--TR

YLFW

LGLI

VLLW

TFTA

GRE-

-----

GQLF

-LAV

LCVL

VTM

CFILV

TKGP

LFLI

FLLW

IGG-

----Y

YLKF

CMY

---HW

VLGI

YSVV

ALCL

NLW

ALEY

QQ

LLLL

IISL-

----C

TVEI

PLN

YVG

YGLG

IIVTA

VSSI

TLLP

HLYV

-----

Hum

an-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

----I

SLSL

VIW

VAIV

GAAS

YFLI

L---

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

---V

TLCL

LSTN

CV-L

DPVI

YCFL

T--

Chic

ken

--TVF

YSIIF

ILGF

VAN

CYVL

WI-

--FM

LNLT

VADL

LFLV

TMPL

WIV

-SV

AGSL

FFVN

TYAS

VAFL

MVI

----

-GIF

LSAA

IWIIT

VGSA

LYYI

F----

AIHI

IICTL

FYVI

FLFI

LTW

N---

RALW

MVC

TVLV

VFIIS

FVPH

H----

---V

TLCL

LSM

NCV

-LDP

VIYC

FLT-

-Ch

impa

nzee

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I----

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

---IH

IFIV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

--VTL

CLLS

TNCV

-LDP

VIYC

FLT-

-Co

w-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

NLA

GCFF

FIN

TYCS

VAFL

AVI--

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

--ILL

SLIIW

VSIV

GAAS

YFFV

L---

-IHIF

LVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

---V

TLCL

LSTN

CV-L

DPIIY

CFLT

--El

epha

nt-F

PIFY

SIIF

VLGV

IAN

GYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

LFFI

NTY

CSVA

FLGV

I----

--ITL

SLVI

WVT

IVAA

ASYF

LFM

-----H

IFIV

CSFF

LVFF

IILVC

NLV

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-QVT

LCLL

STN

CV-L

DPVI

YCFL

---Fr

og---

VTYS

IIFVF

GFIA

NCY

VLW

IF-IF

MIN

LTIA

DLLF

LVTL

PLW

I--N

VAGC

FFFL

NTY

CSVA

FLGV

I----

---CI

SVAV

WIL

LFAS

SLYF

LIFP

----H

FILV

GAFF

VVFL

IILTC

NLV

-RA

LWM

VVTV

LAVF

VLCF

VPHH

-----

----L

CLM

STN

CM-L

DPIIY

CFLT

KKGo

at-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

FFFI

NTY

CSVA

FLAV

I----

--FLL

SLIIW

VSIV

GAAS

YFFV

L---

-IHIF

LVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFVI

CFVP

HH---

----V

TLCL

LSTN

CV-L

DPIIY

CFLT

--Gu

inea

pig

-FPI

VYSI

IFVL

GIIA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

----I

ALSL

VIW

VAIV

AAAS

YFLV

M---

-IHIC

IVLG

FFIV

FLLI

LFCN

L--

RALW

MVC

TVLA

VFVI

CFVP

HH---

---Q

VTLC

LLST

NCV

-LDP

VIYC

FL---

Hedg

ehog

-FPI

FYSI

IFAL

GVIA

NSY

VLW

----F

MVN

LTLA

DLLF

LVTL

PLW

II-N

LAGC

FFFI

NTY

CSVA

FLGV

I----

-GIIL

SLVI

WVA

IVSA

ASYF

FF---

--IH

IFLV

VSFF

VVFL

IIFFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-QIT

LCLL

STN

CV-L

DPIIY

CFL-

--Ho

rse

--PIV

YSIIF

VLGV

IAN

SYVL

WV-

-IFM

VNLT

VADL

LFLL

TLPL

WV-

-N

LTGC

CFFI

NTY

CSVA

FLGV

I----

--ISL

SLVI

WLA

IVAA

ASYF

LFM

----IH

IFLV

FSFF

LVFL

IILVC

NL-

--Q

VTLC

LLST

NCV

-LDP

IIYCF

L---

RALW

MVC

TVLA

VFIIC

FVPH

H----

-M

edak

a-F

SVVY

SIFF

VVGL

LAN

VYVL

F--

--YM

TNLT

IADL

LFVC

ALPF

WIG

---T

GSFF

FIN

TYGT

ILFL

GAIS

L--

RGIIV

SVLI

WLC

LVSS

AIPS

L----

---H

FLIIG

LFFV

VFFL

VMVC

NLL

----

QM

LLAV

VGVF

LLCF

LPHH

VVQ

---Q

ITLV

LMGL

NCI

-LDP

VIYY

FA---

Mon

key

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

I----

--ISL

SLVI

WVA

IVGA

ASYF

FIL-

---IH

IFIV

FSFF

LVFL

IILFC

NL-

--Q

VTLC

LLST

NCV

-LDP

VIYC

FL---

RALW

MVC

TVLA

VFIIC

FVPH

H----

-M

ouse

-FPI

VYSV

IFIL

GVVA

NGY

VLW

----F

MVN

LTM

ADLL

FLIT

LPLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---G

ISLS

LIIW

VSIV

ATAS

YFLA

----

VVHV

FIAF

CFFL

VFFL

IFYC

N---

RALW

MVC

TVLA

VFIIC

FVPH

H----

---I

TLCL

LSTN

CV-L

DPVI

YCFL

T--

Opo

ssum

-FPI

VYSI

IFVL

GFVS

NAY

VLW

-----

MVN

LTVA

DLLF

LFAL

PLW

IVY

NVA

GCLF

FIN

TYCS

VCFL

GVI--

----H

VFIV

LCFF

IVFL

VILV

CNVI

--G

ILLS

TIIW

VVIV

GCAS

YFLI

----

--LW

MVC

TVM

AVFF

ICFV

PHHV

V---

-QVT

LCLM

SIN

CV-L

DPIIY

CFL-

--O

rang

utan

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I----

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

---IH

IFIV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

--VTL

CLLS

TNCV

-LDP

VIYC

FLT-

-Pi

g-F

PIFY

SIIF

VLGV

IAN

SYVL

W--

---M

LNLT

MAD

LLFL

VTLP

LWIIY

NLA

GCFF

FIN

TYCS

VSFL

AVI--

----I

SLSL

IIWVA

MVA

AASY

FFVL

----IH

IFLV

FSFF

LVFL

VILF

CNL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

--ITL

CLLS

TNCV

-LDP

IIYCF

LT--

Pika

--FM

VNLT

VADL

LFLI

TLPL

WII-

-FPI

VYSI

IFVL

GVVA

NGY

VLW

--N

VAGC

LFFI

NTY

CSVA

FLGV

I----

--ISL

SLIIW

VVIL

AIAS

YFLI

M---

-IHIF

IVFC

FFVV

FLIIL

VCN

L--

RALW

MVC

TVLG

VFIIC

FVPH

H----

--Q

VTLC

LLST

NCV

-LDP

VIYC

FL---

Plat

ypus

---VA

YSIIF

VLGI

FAN

GYVL

WVF

--FM

VNLT

VADL

LFLV

TMPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I----

-GIV

LSLV

IWVL

IVSS

ALYF

LV---

--IH

IFIV

ASFF

AVFL

LILV

CNL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

--VTL

CLLS

TNCV

-LDP

VIYC

FLT-

-Pu

fferfish

--PSA

YGVI

FILG

LLAN

LYVL

FI-

---M

TNLS

IADL

LFVC

ALPF

WIG

Y---

GSLF

FIN

TYCS

VLFL

GAIS

VN-

-GIII

SVIIW

VLTI

SMAI

PYLV

----

---FA

IIGLF

FVVF

ALVL

VCN

LLI

-----L

LAVV

GVFV

VCFL

PHHV

IQGA

-QIT

LLLM

GLN

CI-L

DPVV

YCFA

---Ra

bbit

--PIV

YSIIF

VLGV

VAN

GYVL

WV-

--FM

VNLT

VADL

LFLI

TLPL

WII-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

----I

ALSL

VIW

VSIS

AAAT

YFLI

L---

-IHIF

IVAS

FFIV

FLIIL

ICN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--Q

VTLC

LLST

NCV

-LDP

VIYC

FL---

Rat

--PIV

YSVI

FVLG

VVAN

GYVL

WV-

--FM

VNLT

VADL

LFLM

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

I----

-GIT

LSLV

IWIS

IAAT

ASYF

LA---

--V

HIFI

TSCF

FLVF

FLIF

YCN

M--

RALW

MVC

TVLA

VFVI

CFVP

HH---

----I

TLCL

LSTN

CV-L

DPVI

YCFL

T--

Shre

w-F

PIFY

SIIF

VLGV

IAN

SYVL

W--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

-N

LTGC

FFFI

NTY

CSVA

FLGV

I----

-GIY

LSLV

IWM

AIVS

AASY

FFF-

----IH

IFLV

CSFF

LVFL

LIFF

CNL-

-RA

LWM

VCTV

LAVF

SICF

VPHH

-----

--VTL

CLLS

TNCV

-LDP

IIYCF

LT--

Squi

rrel

LFPI

IYSI

IFVV

GVIA

NGY

VL---

--FM

LNLT

VADL

LFLI

TLPL

WII-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

-----

YLSL

VIW

VFIV

AAAS

YFLI

LD--

VIHI

FIVF

SFFL

VFLI

ILFC

K---

RALW

MVC

AVLA

VFVI

CFVP

HH---

---Q

VTLC

LLST

NCV

-LDP

VIYC

FL---

Stickleba

ck-F

PVVY

SIIF

ILGI

TAN

LYVL

F--

CIYM

INLT

IGDL

LFVT

FLPF

W---

--SGS

LFFI

NTY

SSVL

FLCA

ISV-

--G

IILSV

FIW

LFTL

ATAI

LFLI

----

--HLT

IVIG

FFLV

FFVV

VVCN

FL-

---Q

MLF

SVVG

VFVV

CFLP

HHAC

Q--

-QIT

WLL

MSL

NCI

-LDP

VVYF

FA---

Zebrafi

sh--P

IFYG

VVFV

LGLI

ANCY

ALYV

--IY

MTN

LTVA

DLLF

VSAL

PFW

I-----

GTFF

FIN

TYGS

ILFL

TVIS

IN-

RGAI

ISVL

IWAF

ILSA

SIPQ

L----

-IT

HFSI

IGLF

FLVF

LMVI

ICN

-----L

RMLC

AVIG

VFVI

CFLP

HHVV

----Q

ITLM

LMGI

NCL

-LDP

LVYC

FA---


Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5083

17.7

0---

SFFI

SFFF

FFFD

STGE

IEGF

V-F

FTLV

LLFC

SWGM

LLQ

MAM

VI--

EFSS

VALG

IAAY

-AVL

FLSE

SS--

KFFL

IPLM

VILA

LHFL

V-HL

MI--

------

-LSR

FLAD

FIM

FFCG

CAVL

LGL

--IAM

TCGC

LMIM

FFLL

IAPS

FF---

KNGV

IPPA

SVLV

LVLD

AVSR

Q---

------

Hum

an-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

-----

RALW

MVC

TVLA

VFIIC

FVPH

H----

----

----V

TLCL

LSTN

CVLD

PVIY

CFLT

--Ch

icke

n--T

VFYS

IIFIL

GFVA

NCY

VLW

I---F

MLN

LTVA

DLLF

LVTM

PLW

IV-

SVAG

SLFF

VNTY

ASVA

FLM

VI---

-GIF

LSAA

IWIIT

VGSA

LYYI

F--

RALW

MVC

TVLV

VFIIS

FVPH

H----

-AI

HIIIC

TLFY

VIFL

FILT

WN

------

----

----V

TLCL

LSM

NCV

LDPV

IYCF

LT--

Chim

panz

ee-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

-----

RALW

MVC

TVLA

VFIIC

FVPH

H----

----

----V

TLCL

LSTN

CVLD

PVIY

CFLT

--Co

w-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

FFFI

NTY

CSVA

FLAV

I---

--ILL

SLIIW

VSIV

GAAS

YFFV

L--IH

IFLV

FSFF

LVFL

IILFC

NL-

-----

RALW

MVC

TVLA

VFIIC

FVPH

H----

----

----V

TLCL

LSTN

CVLD

PIIY

CFLT

--El

epha

nt-F

PIFY

SIIF

VLGV

IAN

GYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

LFFI

NTY

CSVA

FLGV

I---

--ITL

SLVI

WVT

IVAA

ASYF

LFM

---H

IFIV

CSFF

LVFF

IILVC

NLV

-----

RALW

MVC

TVLA

VFIIC

FVPH

H----

----

---Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Frog

---VT

YSIIF

VFGF

IAN

CYVL

WIF

-IFM

INLT

IADL

LFLV

TLPL

WI--

NVA

GCFF

FLN

TYCS

VAFL

GVI--

----

CISV

AVW

ILLF

ASSL

YFLI

FP--H

FILV

GAFF

VVFL

IILTC

NLV

-----

RALW

MVV

TVLA

VFVL

CFVP

HH---

-----

------

LCLM

STN

CMLD

PIIY

CFLT

KKGo

at-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

FFFI

NTY

CSVA

FLAV

I---

--FLL

SLIIW

VSIV

GAAS

YFFV

L--IH

IFLV

FSFF

LVFL

IILFC

NL-

-----

RALW

MVC

TVLA

VFVI

CFVP

HH---

-----

----V

TLCL

LSTN

CVLD

PIIY

CFLT

--Gu

inea

pig

-FPI

VYSI

IFVL

GIIA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

ALSL

VIW

VAIV

AAAS

YFLV

M-

-IHIC

IVLG

FFIV

FLLI

LFCN

L----

--RA

LWM

VCTV

LAVF

VICF

VPHH

-----

------

QVT

LCLL

STN

CVLD

PVIY

CFL-

--He

dgeh

og-F

PIFY

SIIF

ALGV

IAN

SYVL

W--

--FM

VNLT

LADL

LFLV

TLPL

WII-

NLA

GCFF

FIN

TYCS

VAFL

GVI--

--G

IILSL

VIW

VAIV

SAAS

YFFF

---IH

IFLV

VSFF

VVFL

IIFFC

NL-

-----

RALW

MVC

TVLA

VFIIC

FVPH

H----

----

---Q

ITLC

LLST

NCV

LDPI

IYCF

L---

Hors

e--P

IVYS

IIFVL

GVIA

NSY

VLW

V-N

LTGC

CFFI

NTY

CSVA

FLGV

I---

-IFM

VNLT

VADL

LFLL

TLPL

WV-

---I

SLSL

VIW

LAIV

AAAS

YFLF

M-

-IHIF

LVFS

FFLV

FLIIL

VCN

L----

--RA

LWM

VCTV

LAVF

IICFV

PHH-

----

------

QVT

LCLL

STN

CVLD

PIIY

CFL-

--M

edak

a-F

SVVY

SIFF

VVGL

LAN

VYVL

F--

--YM

TNLT

IADL

LFVC

ALPF

WIG

-RG

IIVSV

LIW

LCLV

SSAI

PSL-

----T

GSFF

FIN

TYGT

ILFL

GAIS

L---H

FLIIG

LFFV

VFFL

VMVC

NLL

-----

---Q

MLL

AVVG

VFLL

CFLP

HHVV

Q--

------

QIT

LVLM

GLN

CILD

PVIY

YFA-

--M

onke

y-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFFI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

-----

RALW

MVC

TVLA

VFIIC

FVPH

H----

----

---Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Mou

se-F

PIVY

SVIF

ILGV

VAN

GYVL

W--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

-GIS

LSLI

IWVS

IVAT

ASYF

LA--

VVHV

FIAF

CFFL

VFFL

IFYC

N---

----

RALW

MVC

TVLA

VFIIC

FVPH

H----

----

----IT

LCLL

STN

CVLD

PVIY

CFLT

--O

poss

um-F

PIVY

SIIF

VLGF

VSN

AYVL

W--

---M

VNLT

VADL

LFLF

ALPL

WIV

YN

VAGC

LFFI

NTY

CSVC

FLGV

I---

--HVF

IVLC

FFIV

FLVI

LVCN

VI---

---G

ILLS

TIIW

VVIV

GCAS

YFLI

----L

WM

VCTV

MAV

FFIC

FVPH

HVV-

-----

---Q

VTLC

LMSI

NCV

LDPI

IYCF

L---

Ora

ngut

an-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

-----

RALW

MVC

TVLA

VFIIC

FVPH

H----

----

----V

TLCL

LSTN

CVLD

PVIY

CFLT

--Pi

g-F

PIFY

SIIF

VLGV

IAN

SYVL

W--

---M

LNLT

MAD

LLFL

VTLP

LWIIY

NLA

GCFF

FIN

TYCS

VSFL

AVI--

---I

SLSL

IIWVA

MVA

AASY

FFVL

--IH

IFLV

FSFF

LVFL

VILF

CNL-

-----

RALW

MVC

TVLA

VFIIC

FVPH

H----

----

----IT

LCLL

STN

CVLD

PIIY

CFLT

--Pi

ka-F

PIVY

SIIF

VLGV

VAN

GYVL

W--

--FM

VNLT

VADL

LFLI

TLPL

WII-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

IIWVV

ILAI

ASYF

LIM

--IH

IFIV

FCFF

VVFL

IILVC

NL-

-----

------

QVT

LCLL

STN

CVLD

PVIY

CFL-

--RA

LWM

VCTV

LGVF

IICFV

PHH-

----

Plat

ypus

---VA

YSIIF

VLGI

FAN

GYVL

WVF

--FM

VNLT

VADL

LFLV

TMPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

-GIV

LSLV

IWVL

IVSS

ALYF

LV--

-IHIF

IVAS

FFAV

FLLI

LVCN

L----

--RA

LWM

VCTV

LAVF

IICFV

PHH-

----

------

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Puffe

rfish

---M

TNLS

IADL

LFVC

ALPF

WIG

Y--P

SAYG

VIFI

LGLL

ANLY

VLFI

----

GSLF

FIN

TYCS

VLFL

GAIS

VN-G

IIISV

IIWVL

TISM

AIPY

LV--

---FA

IIGLF

FVVF

ALVL

VCN

LLI--

-----

--LLA

VVGV

FVVC

FLPH

HVIQ

GA---

---Q

ITLL

LMGL

NCI

LDPV

VYCF

A---

Rabb

it--P

IVYS

IIFVL

GVVA

NGY

VLW

V---F

MVN

LTVA

DLLF

LITL

PLW

II-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--IAL

SLVI

WVS

ISAA

ATYF

LIL-

-IHIF

IVAS

FFIV

FLIIL

ICN

L----

--RA

LWM

VCTV

LAVF

IICFV

PHH-

----

------

QVT

LCLL

STN

CVLD

PVIY

CFL-

--Ra

t--P

IVYS

VIFV

LGVV

ANGY

VLW

V---F

MVN

LTVA

DLLF

LMTL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

--G

ITLS

LVIW

ISIA

ATAS

YFLA

---V

HIFI

TSCF

FLVF

FLIF

YCN

M---

---RA

LWM

VCTV

LAVF

VICF

VPHH

-----

------

-ITLC

LLST

NCV

LDPV

IYCF

LT--

Shre

w-F

PIFY

SIIF

VLGV

IAN

SYVL

W--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

-N

LTGC

FFFI

NTY

CSVA

FLGV

I---

-GIY

LSLV

IWM

AIVS

AASY

FFF-

--IH

IFLV

CSFF

LVFL

LIFF

CNL-

-----

RALW

MVC

TVLA

VFSI

CFVP

HH---

-----

----V

TLCL

LSTN

CVLD

PIIY

CFLT

--Sq

uirr

elLF

PIIY

SIIF

VVGV

IAN

GYVL

-----F

MLN

LTVA

DLLF

LITL

PLW

II-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

---YL

SLVI

WVF

IVAA

ASYF

LILD

VIHI

FIVF

SFFL

VFLI

ILFC

K----

---RA

LWM

VCAV

LAVF

VICF

VPHH

-----

------

QVT

LCLL

STN

CVLD

PVIY

CFL-

--Sti

ckleba

ck-F

PVVY

SIIF

ILGI

TAN

LYVL

F--

CIYM

INLT

IGDL

LFVT

FLPF

W---

--SGS

LFFI

NTY

SSVL

FLCA

ISV-

-GIIL

SVFI

WLF

TLAT

AILF

LI--

--HLT

IVIG

FFLV

FFVV

VVCN

FL---

-----

QM

LFSV

VGVF

VVCF

LPHH

ACQ

-----

---Q

ITW

LLM

SLN

CILD

PVVY

FFA-

--Zebrafi

sh--P

IFYG

VVFV

LGLI

ANCY

ALYV

--IY

MTN

LTVA

DLLF

VSAL

PFW

I--RG

AIIS

VLIW

AFIL

SASI

PQL-

-----

GTFF

FIN

TYGS

ILFL

TVIS

INIT

HFSI

IGLF

FLVF

LMVI

ICN

------

---L

RMLC

AVIG

VFVI

CFLP

HHVV

------

---Q

ITLM

LMGI

NCL

LDPL

VYCF

A---

Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5098

49.3

0--Q

IVLL

LLLL

LLLL

LLLL

LLLL

-IF

FTLV

LLFC

SWGM

LLQ

MAM

V---

REFS

SVAL

GIAA

HAVL

LLSE

N---

----F

FLIP

FTVI

LAL-

-HFL

VHLM

IS---

----L

SRFL

ADFI

MFF

CGCA

VLLG

L--I

AMTC

GCLM

IMFF

LLIA

PSFF

---KN

GVIP

PASV

LVLV

LDAV

SRQ

------

---Hu

man

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

----IH

IFIV

FSFF

LVFL

IILFC

NL-

-----

RALW

MVC

TVLA

VFIIC

FVPH

H----

----

----V

TLCL

LSTN

CVLD

PVIY

CFLT

--Ch

icke

n--T

VFYS

IIFIL

GFVA

NCY

VLW

I---F

MLN

LTVA

DLLF

LVTM

PLW

IV-

SVAG

SLFF

VNTY

ASVA

FLM

VI---

-GIF

LSAA

IWIIT

VGSA

LYYI

F----

-AI

HIIIC

TLFY

VIFL

FILT

WN

------

-RA

LWM

VCTV

LVVF

IISFV

PHH-

----

------

-VTL

CLLS

MN

CVLD

PVIY

CFLT

--Ch

impa

nzee

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

----IH

IFIV

FSFF

LVFL

IILFC

NL-

-----

RALW

MVC

TVLA

VFIIC

FVPH

H----

----

----V

TLCL

LSTN

CVLD

PVIY

CFLT

--Co

w-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

FFFI

NTY

CSVA

FLAV

I---

--ILL

SLIIW

VSIV

GAAS

YFFV

L----

-IHIF

LVFS

FFLV

FLIIL

FCN

L----

--RA

LWM

VCTV

LAVF

IICFV

PHH-

----

------

-VTL

CLLS

TNCV

LDPI

IYCF

LT--

Elep

hant

-FPI

FYSI

IFVL

GVIA

NGY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

TLSL

VIW

VTIV

AAAS

YFLF

M---

---H

IFIV

CSFF

LVFF

IILVC

NLV

-----

RALW

MVC

TVLA

VFIIC

FVPH

H----

----

---Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Frog

---VT

YSIIF

VFGF

IAN

CYVL

WIF

-IFM

INLT

IADL

LFLV

TLPL

WI--

NVA

GCFF

FLN

TYCS

VAFL

GVI--

----

CISV

AVW

ILLF

ASSL

YFLI

FP---

--HFI

LVGA

FFVV

FLIIL

TCN

LV---

--RA

LWM

VVTV

LAVF

VLCF

VPHH

-----

------

---LC

LMST

NCM

LDPI

IYCF

LTKK

Goat

-FPI

FYSI

VFVL

GVIA

NSY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCFF

FIN

TYCS

VAFL

AVI--

---F

LLSL

IIWVS

IVGA

ASYF

FVL-

----IH

IFLV

FSFF

LVFL

IILFC

NL-

-----

RALW

MVC

TVLA

VFVI

CFVP

HH---

-----

----V

TLCL

LSTN

CVLD

PIIY

CFLT

--Gu

inea

pig

-FPI

VYSI

IFVL

GIIA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

ALSL

VIW

VAIV

AAAS

YFLV

M---

--IH

ICIV

LGFF

IVFL

LILF

CNL-

-----

RALW

MVC

TVLA

VFVI

CFVP

HH---

-----

---Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Hedg

ehog

-FPI

FYSI

IFAL

GVIA

NSY

VLW

----F

MVN

LTLA

DLLF

LVTL

PLW

II-N

LAGC

FFFI

NTY

CSVA

FLGV

I---

-GIIL

SLVI

WVA

IVSA

ASYF

FF---

---IH

IFLV

VSFF

VVFL

IIFFC

NL-

-----

RALW

MVC

TVLA

VFIIC

FVPH

H----

----

---Q

ITLC

LLST

NCV

LDPI

IYCF

L---

Hors

e--P

IVYS

IIFVL

GVIA

NSY

VLW

V--IF

MVN

LTVA

DLLF

LLTL

PLW

V--

NLT

GCCF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

LAIV

AAAS

YFLF

M---

--IH

IFLV

FSFF

LVFL

IILVC

NL-

-----

RALW

MVC

TVLA

VFIIC

FVPH

H----

----

---Q

VTLC

LLST

NCV

LDPI

IYCF

L---

Med

aka

-FSV

VYSI

FFVV

GLLA

NVY

VLF-

---Y

MTN

LTIA

DLLF

VCAL

PFW

IG-

--TGS

FFFI

NTY

GTIL

FLGA

ISL-

RGIIV

SVLI

WLC

LVSS

AIPS

L----

----H

FLIIG

LFFV

VFFL

VMVC

NLL

-----

---Q

MLL

AVVG

VFLL

CFLP

HHVV

Q--

------

QIT

LVLM

GLN

CILD

PVIY

YFA-

--M

onke

y-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFFI

L----

-IHIF

IVFS

FFLV

FLIIL

FCN

L----

--RA

LWM

VCTV

LAVF

IICFV

PHH-

----

------

QVT

LCLL

STN

CVLD

PVIY

CFL-

--M

ouse

-FPI

VYSV

IFIL

GVVA

NGY

VLW

----F

MVN

LTM

ADLL

FLIT

LPLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

--G

ISLS

LIIW

VSIV

ATAS

YFLA

-----

VVHV

FIAF

CFFL

VFFL

IFYC

N---

----

RALW

MVC

TVLA

VFIIC

FVPH

H----

----

----IT

LCLL

STN

CVLD

PVIY

CFLT

--O

poss

um-F

PIVY

SIIF

VLGF

VSN

AYVL

W--

---M

VNLT

VADL

LFLF

ALPL

WIV

YN

VAGC

LFFI

NTY

CSVC

FLGV

I---

-GIL

LSTI

IWVV

IVGC

ASYF

LI---

----H

VFIV

LCFF

IVFL

VILV

CNVI

-----

--LW

MVC

TVM

AVFF

ICFV

PHHV

V---

------

QVT

LCLM

SIN

CVLD

PIIY

CFL-

--O

rang

utan

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

----IH

IFIV

FSFF

LVFL

IILFC

NL-

-----

RALW

MVC

TVLA

VFIIC

FVPH

H----

----

----V

TLCL

LSTN

CVLD

PVIY

CFLT

--Pi

g-F

PIFY

SIIF

VLGV

IAN

SYVL

W--

---M

LNLT

MAD

LLFL

VTLP

LWIIY

NLA

GCFF

FIN

TYCS

VSFL

AVI--

---I

SLSL

IIWVA

MVA

AASY

FFVL

----

-IHIF

LVFS

FFLV

FLVI

LFCN

L----

--RA

LWM

VCTV

LAVF

IICFV

PHH-

----

------

-ITLC

LLST

NCV

LDPI

IYCF

LT--

Pika

-FPI

VYSI

IFVL

GVVA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

II-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLIIW

VVIL

AIAS

YFLI

M---

--IH

IFIV

FCFF

VVFL

IILVC

NL-

-----

RALW

MVC

TVLG

VFIIC

FVPH

H----

----

---Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Plat

ypus

---VA

YSIIF

VLGI

FAN

GYVL

WVF

--FM

VNLT

VADL

LFLV

TMPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

-IHIF

IVAS

FFAV

FLLI

LVCN

L----

---G

IVLS

LVIW

VLIV

SSAL

YFLV

-----

RALW

MVC

TVLA

VFIIC

FVPH

H----

----

----V

TLCL

LSTN

CVLD

PVIY

CFLT

--Pu

fferfish

--PSA

YGVI

FILG

LLAN

LYVL

FI-

---M

TNLS

IADL

LFVC

ALPF

WIG

Y---

GSLF

FIN

TYCS

VLFL

GAIS

VN-G

IIISV

IIWVL

TISM

AIPY

LV---

-----

FAIIG

LFFV

VFAL

VLVC

NLL

I----

-----L

LAVV

GVFV

VCFL

PHHV

IQGA

------

QIT

LLLM

GLN

CILD

PVVY

CFA-

--Ra

bbit

--PIV

YSIIF

VLGV

VAN

GYVL

WV-

--FM

VNLT

VADL

LFLI

TLPL

WII-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

ALSL

VIW

VSIS

AAAT

YFLI

L----

-IHIF

IVAS

FFIV

FLIIL

ICN

L----

--RA

LWM

VCTV

LAVF

IICFV

PHH-

----

------

QVT

LCLL

STN

CVLD

PVIY

CFL-

--Ra

t--P

IVYS

VIFV

LGVV

ANGY

VLW

V---F

MVN

LTVA

DLLF

LMTL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

--G

ITLS

LVIW

ISIA

ATAS

YFLA

-----

-VHI

FITS

CFFL

VFFL

IFYC

NM

------

RALW

MVC

TVLA

VFVI

CFVP

HH---

-----

----IT

LCLL

STN

CVLD

PVIY

CFLT

--Sh

rew

-FPI

FYSI

IFVL

GVIA

NSY

VLW

----F

MVN

LTM

ADLL

FLIT

LPLW

IV-

NLT

GCFF

FIN

TYCS

VAFL

GVI--

--G

IYLS

LVIW

MAI

VSAA

SYFF

F----

--IH

IFLV

CSFF

LVFL

LIFF

CNL-

-----

RALW

MVC

TVLA

VFSI

CFVP

HH---

-----

----V

TLCL

LSTN

CVLD

PIIY

CFLT

--Sq

uirr

el--F

MLN

LTVA

DLLF

LITL

PLW

II-LF

PIIY

SIIF

VVGV

IAN

GYVL

---N

VAGC

LFFI

NTY

CSVA

FLGV

I---

---YL

SLVI

WVF

IVAA

ASYF

LILD

---VI

HIFI

VFSF

FLVF

LIIL

FCK-

------

RALW

MVC

AVLA

VFVI

CFVP

HH---

-----

---Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Stickleba

ck-F

PVVY

SIIF

ILGI

TAN

LYVL

F--

--SGS

LFFI

NTY

SSVL

FLCA

ISV-

CIYM

INLT

IGDL

LFVT

FLPF

W---

-GIIL

SVFI

WLF

TLAT

AILF

LI---

-----

QM

LFSV

VGVF

VVCF

LPHH

ACQ

----H

LTIV

IGFF

LVFF

VVVV

CNFL

-----

------

QIT

WLL

MSL

NCI

LDPV

VYFF

A---

Zebrafi

sh-IY

MTN

LTVA

DLLF

VSAL

PFW

I----P

IFYG

VVFV

LGLI

ANCY

ALYV

----

GTFF

FIN

TYGS

ILFL

TVIS

INRG

AIIS

VLIW

AFIL

SASI

PQL-

-----

--LRM

LCAV

IGVF

VICF

LPHH

VV---

ITHF

SIIG

LFFL

VFLM

VIIC

N---

----

------

QIT

LMLM

GIN

CLLD

PLVY

CFA-

--


Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5107

29.2

90---

-TDV

CIFI

LNFL

SLLV

AFKL

YL-

--ELQ

PFSC

CPLI

GKIIF

GVW

DR-

---W

IRYF

TTYF

IVM

LFRA

IVVV

M-N

PVKN

VIM

TLVT

FGSG

--FIH

CG-F

SGHT

VPIT

LSLL

VQW

IYGS

M--

-----V

FRPA

SVLL

VLLS

FYSI

IASR

YTDD

ILVSF

YITV

TTFL

VLRH

---Hu

man

-FPI

VYSI

IFVL

GVIA

--NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

--Ch

icke

n--T

VFYS

IIFIL

GFVA

--NCY

VLW

I---F

MLN

LTVA

DLLF

LVTM

PLW

IV-

SVAG

SLFF

VNTY

ASVA

FLM

VI---

-GIF

LSAA

IWIIT

VGSA

LYYI

F--

AIHI

IICTL

FYVI

FLFI

LTW

N---

RALW

MVC

TVLV

VFIIS

FVPH

H----

--V

TLCL

LSM

NCV

LDPV

IYCF

LT--

Chim

panz

ee-F

PIVY

SIIF

VLGV

IA--N

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Cow

-FPI

FYSI

VFVL

GVIA

--NSY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCFF

FIN

TYCS

VAFL

AVI--

---I

LLSL

IIWVS

IVGA

ASYF

FVL-

-IHIF

LVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PIIY

CFLT

--El

epha

nt-F

PIFY

SIIF

VLGV

IA--N

GYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

LFFI

NTY

CSVA

FLGV

I---

--ITL

SLVI

WVT

IVAA

ASYF

LFM

---H

IFIV

CSFF

LVFF

IILVC

NLV

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--Fr

og---

VTYS

IIFVF

GFIA

--NCY

VLW

IF-IF

MIN

LTIA

DLLF

LVTL

PLW

I--N

VAGC

FFFL

NTY

CSVA

FLGV

I---

---CI

SVAV

WIL

LFAS

SLYF

LIFP

--HFI

LVGA

FFVV

FLIIL

TCN

LV-

RALW

MVV

TVLA

VFVL

CFVP

HH---

-----

LCLM

STN

CMLD

PIIY

CFLT

KKGo

at-F

PIFY

SIVF

VLGV

IA--N

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

FFFI

NTY

CSVA

FLAV

I---

--FLL

SLIIW

VSIV

GAAS

YFFV

L--IH

IFLV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

VICF

VPHH

-----

-VTL

CLLS

TNCV

LDPI

IYCF

LT--

Guin

ea p

ig-F

PIVY

SIIF

VLGI

IA--N

GYVL

W--

--FM

VNLT

VADL

LFLI

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--IAL

SLVI

WVA

IVAA

ASYF

LVM

--IH

ICIV

LGFF

IVFL

LILF

CNL-

-RA

LWM

VCTV

LAVF

VICF

VPHH

-----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--He

dgeh

og-F

PIFY

SIIF

ALGV

IA--N

SYVL

W--

--FM

VNLT

LADL

LFLV

TLPL

WII-

NLA

GCFF

FIN

TYCS

VAFL

GVI--

--G

IILSL

VIW

VAIV

SAAS

YFFF

---IH

IFLV

VSFF

VVFL

IIFFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

QIT

LCLL

STN

CVLD

PIIY

CFL-

--Ho

rse

--PIV

YSIIF

VLGV

IA--N

SYVL

WV-

-IFM

VNLT

VADL

LFLL

TLPL

WV-

-N

LTGC

CFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WLA

IVAA

ASYF

LFM

--IH

IFLV

FSFF

LVFL

IILVC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PIIY

CFL-

--M

edak

a-F

SVVY

SIFF

VVGL

LA--N

VYVL

F--

--YM

TNLT

IADL

LFVC

ALPF

WIG

---T

GSFF

FIN

TYGT

ILFL

GAIS

L-RG

IIVSV

LIW

LCLV

SSAI

PSL-

----H

FLIIG

LFFV

VFFL

VMVC

NLL

----

QM

LLAV

VGVF

LLCF

LPHH

VVQ

--Q

ITLV

LMGL

NCI

LDPV

IYYF

A---

Mon

key

-FPI

VYSI

IFVL

GVIA

--NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

FIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Mou

se-F

PIVY

SVIF

ILGV

VA--N

GYVL

W--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

-GIS

LSLI

IWVS

IVAT

ASYF

LA--

VVHV

FIAF

CFFL

VFFL

IFYC

N---

RALW

MVC

TVLA

VFIIC

FVPH

H----

--IT

LCLL

STN

CVLD

PVIY

CFLT

--O

poss

um-F

PIVY

SIIF

VLGF

VS--N

AYVL

W--

---M

VNLT

VADL

LFLF

ALPL

WIV

YN

VAGC

LFFI

NTY

CSVC

FLGV

I---

-GIL

LSTI

IWVV

IVGC

ASYF

LI--

--HVF

IVLC

FFIV

FLVI

LVCN

VI-

--LW

MVC

TVM

AVFF

ICFV

PHHV

V---

QVT

LCLM

SIN

CVLD

PIIY

CFL-

--O

rang

utan

-FPI

VYSI

IFVL

GVIA

--NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

--Pi

g-F

PIFY

SIIF

VLGV

IA--N

SYVL

W--

---M

LNLT

MAD

LLFL

VTLP

LWIIY

NLA

GCFF

FIN

TYCS

VSFL

AVI--

---I

SLSL

IIWVA

MVA

AASY

FFVL

--IH

IFLV

FSFF

LVFL

VILF

CNL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-ITLC

LLST

NCV

LDPI

IYCF

LT--

Pika

-FPI

VYSI

IFVL

GVVA

--NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

II-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLIIW

VVIL

AIAS

YFLI

M-

-IHIF

IVFC

FFVV

FLIIL

VCN

L--

RALW

MVC

TVLG

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Plat

ypus

---VA

YSIIF

VLGI

FA--N

GYVL

WVF

--FM

VNLT

VADL

LFLV

TMPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

-GIV

LSLV

IWVL

IVSS

ALYF

LV--

-IHIF

IVAS

FFAV

FLLI

LVCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

--Pu

fferfish

--PSA

YGVI

FILG

LLA-

-NLY

VLFI

----

MTN

LSIA

DLLF

VCAL

PFW

IGY

---GS

LFFI

NTY

CSVL

FLGA

ISVN

-GIII

SVIIW

VLTI

SMAI

PYLV

-----

FAIIG

LFFV

VFAL

VLVC

NLL

I---

--LLA

VVGV

FVVC

FLPH

HVIQ

GAQ

ITLL

LMGL

NCI

LDPV

VYCF

A---

Rabb

it--P

IVYS

IIFVL

GVVA

--NGY

VLW

V---F

MVN

LTVA

DLLF

LITL

PLW

II-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--IAL

SLVI

WVS

ISAA

ATYF

LIL-

-IHIF

IVAS

FFIV

FLIIL

ICN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Rat

--PIV

YSVI

FVLG

VVA-

-NGY

VLW

V---F

MVN

LTVA

DLLF

LMTL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

--G

ITLS

LVIW

ISIA

ATAS

YFLA

---V

HIFI

TSCF

FLVF

FLIF

YCN

M--

RALW

MVC

TVLA

VFVI

CFVP

HH---

---IT

LCLL

STN

CVLD

PVIY

CFLT

--Sh

rew

-FPI

FYSI

IFVL

GVIA

--NSY

VLW

----F

MVN

LTM

ADLL

FLIT

LPLW

IV-

NLT

GCFF

FIN

TYCS

VAFL

GVI--

--G

IYLS

LVIW

MAI

VSAA

SYFF

F--

-IHIF

LVCS

FFLV

FLLI

FFCN

L--

RALW

MVC

TVLA

VFSI

CFVP

HH---

---V

TLCL

LSTN

CVLD

PIIY

CFLT

--Sq

uirr

elLF

PIIY

SIIF

VVGV

IA--N

GYVL

-----F

MLN

LTVA

DLLF

LITL

PLW

II-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

---YL

SLVI

WVF

IVAA

ASYF

LILD

VIHI

FIVF

SFFL

VFLI

ILFC

K---

RALW

MVC

AVLA

VFVI

CFVP

HH---

--Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Stickleba

ck-F

PVVY

SIIF

ILGI

TA--N

LYVL

F--

CIYM

INLT

IGDL

LFVT

FLPF

W---

--SGS

LFFI

NTY

SSVL

FLCA

ISV-

-GIIL

SVFI

WLF

TLAT

AILF

LI--

--HLT

IVIG

FFLV

FFVV

VVCN

FL-

---Q

MLF

SVVG

VFVV

CFLP

HHAC

Q--

QIT

WLL

MSL

NCI

LDPV

VYFF

A---

Zebrafi

sh--P

IFYG

VVFV

LGLI

A--N

CYAL

YV-

-IYM

TNLT

VADL

LFVS

ALPF

WI--

---GT

FFFI

NTY

GSIL

FLTV

ISIN

RGAI

ISVL

IWAF

ILSA

SIPQ

L---

ITHF

SIIG

LFFL

VFLM

VIIC

N---

--LRM

LCAV

IGVF

VICF

LPHH

VV---

QIT

LMLM

GIN

CLLD

PLVY

CFA-

--

Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5041

37.1

40SH

GVGL

ILFG

VLSI

VVFD

ALL-

---F

IYGT

FCFA

CMM

CMGC

SSIY

H--

YYGI

SVLI

VASF

LPPL

YIGF

Y---

QAL

YMGC

ACVF

GGLC

LAM

AAL-

--RI

MTY

VATV

MCG

LVPT

LHYV

F----

-Q

TFVG

VFLM

FLFY

GMGV

LFYC

------

----

QIW

HVFV

LVAA

CV--H

FFTC

ISI--

Hum

an-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L----

IHIF

IVFS

FFLV

FLIIL

FCN

L--

-----R

ALW

MVC

TVLA

VFIIC

FVPH

H----

--V

-TLC

LLST

NCV

LDPV

IYCF

LT--

Chic

ken

--TVF

YSIIF

ILGF

VAN

CYVL

WI-

--FM

LNLT

VADL

LFLV

TMPL

WIV

-SV

AGSL

FFVN

TYAS

VAFL

MVI

----G

IFLS

AAIW

IITVG

SALY

YIF-

---A

IHIII

CTLF

YVIF

LFILT

WN

------

--RAL

WM

VCTV

LVVF

IISFV

PHH-

----

-V-T

LCLL

SMN

CVLD

PVIY

CFLT

--Ch

impa

nzee

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

---I

SLSL

VIW

VAIV

GAAS

YFLI

L-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

---IH

IFIV

FSFF

LVFL

IILFC

NL-

--V

-TLC

LLST

NCV

LDPV

IYCF

LT--

-----R

ALW

MVC

TVLA

VFIIC

FVPH

H----

-Co

w-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

FFFI

NTY

CSVA

FLAV

I---

--ILL

SLIIW

VSIV

GAAS

YFFV

L----

IHIF

LVFS

FFLV

FLIIL

FCN

L--

-----R

ALW

MVC

TVLA

VFIIC

FVPH

H----

--V

-TLC

LLST

NCV

LDPI

IYCF

LT--

Elep

hant

-FPI

FYSI

IFVL

GVIA

NGY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

TLSL

VIW

VTIV

AAAS

YFLF

M-

----H

IFIV

CSFF

LVFF

IILVC

NLV

----

--RAL

WM

VCTV

LAVF

IICFV

PHH-

----

QV-

TLCL

LSTN

CVLD

PVIY

CFL-

--Fr

og---

VTYS

IIFVF

GFIA

NCY

VLW

IF-IF

MIN

LTIA

DLLF

LVTL

PLW

I--N

VAGC

FFFL

NTY

CSVA

FLGV

I---

---CI

SVAV

WIL

LFAS

SLYF

LIFP

----H

FILV

GAFF

VVFL

IILTC

NLV

----

--RAL

WM

VVTV

LAVF

VLCF

VPHH

-----

----L

CLM

STN

CMLD

PIIY

CFLT

KKGo

at-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

FFFI

NTY

CSVA

FLAV

I---

---IH

IFLV

FSFF

LVFL

IILFC

NL-

---F

LLSL

IIWVS

IVGA

ASYF

FVL-

-----R

ALW

MVC

TVLA

VFVI

CFVP

HH---

---V

-TLC

LLST

NCV

LDPI

IYCF

LT--

Guin

ea p

ig-F

PIVY

SIIF

VLGI

IAN

GYVL

W--

--FM

VNLT

VADL

LFLI

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--IAL

SLVI

WVA

IVAA

ASYF

LVM

----

IHIC

IVLG

FFIV

FLLI

LFCN

L--

-----R

ALW

MVC

TVLA

VFVI

CFVP

HH---

--Q

V-TL

CLLS

TNCV

LDPV

IYCF

L---

Hedg

ehog

-FPI

FYSI

IFAL

GVIA

NSY

VLW

--N

LAGC

FFFI

NTY

CSVA

FLGV

I---

--FM

VNLT

LADL

LFLV

TLPL

WII-

-GIIL

SLVI

WVA

IVSA

ASYF

FF--

---IH

IFLV

VSFF

VVFL

IIFFC

NL-

----

--RAL

WM

VCTV

LAVF

IICFV

PHH-

----

QI-T

LCLL

STN

CVLD

PIIY

CFL-

--Ho

rse

--PIV

YSIIF

VLGV

IAN

SYVL

WV-

-IFM

VNLT

VADL

LFLL

TLPL

WV-

-N

LTGC

CFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WLA

IVAA

ASYF

LFM

----

IHIF

LVFS

FFLV

FLIIL

VCN

L--

-----R

ALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

V-TL

CLLS

TNCV

LDPI

IYCF

L---

Med

aka

-FSV

VYSI

FFVV

GLLA

NVY

VLF-

---Y

MTN

LTIA

DLLF

VCAL

PFW

IG-

--TGS

FFFI

NTY

GTIL

FLGA

ISL-

RGIIV

SVLI

WLC

LVSS

AIPS

L---

----H

FLIIG

LFFV

VFFL

VMVC

NLL

----

-----Q

MLL

AVVG

VFLL

CFLP

HHVV

Q--

QI-T

LVLM

GLN

CILD

PVIY

YFA-

--M

onke

y-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFFI

L----

IHIF

IVFS

FFLV

FLIIL

FCN

L--

-----R

ALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

V-TL

CLLS

TNCV

LDPV

IYCF

L---

Mou

se-F

PIVY

SVIF

ILGV

VAN

GYVL

W--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

-GIS

LSLI

IWVS

IVAT

ASYF

LA--

--VVH

VFIA

FCFF

LVFF

LIFY

CN---

-----R

ALW

MVC

TVLA

VFIIC

FVPH

H----

--I-

TLCL

LSTN

CVLD

PVIY

CFLT

--O

poss

um-F

PIVY

SIIF

VLGF

VSN

AYVL

W--

---M

VNLT

VADL

LFLF

ALPL

WIV

YN

VAGC

LFFI

NTY

CSVC

FLGV

I---

-GIL

LSTI

IWVV

IVGC

ASYF

LI--

----H

VFIV

LCFF

IVFL

VILV

CNVI

----

----L

WM

VCTV

MAV

FFIC

FVPH

HVV-

--Q

V-TL

CLM

SIN

CVLD

PIIY

CFL-

--O

rang

utan

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

---IH

IFIV

FSFF

LVFL

IILFC

NL-

----

--RAL

WM

VCTV

LAVF

IICFV

PHH-

----

-V-T

LCLL

STN

CVLD

PVIY

CFLT

--Pi

g-F

PIFY

SIIF

VLGV

IAN

SYVL

W--

---M

LNLT

MAD

LLFL

VTLP

LWIIY

NLA

GCFF

FIN

TYCS

VSFL

AVI--

---I

SLSL

IIWVA

MVA

AASY

FFVL

----

IHIF

LVFS

FFLV

FLVI

LFCN

L--

-----R

ALW

MVC

TVLA

VFIIC

FVPH

H----

--I-

TLCL

LSTN

CVLD

PIIY

CFLT

--Pi

ka-F

PIVY

SIIF

VLGV

VAN

GYVL

W--

--FM

VNLT

VADL

LFLI

TLPL

WII-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

IIWVV

ILAI

ASYF

LIM

----

IHIF

IVFC

FFVV

FLIIL

VCN

L--

-----R

ALW

MVC

TVLG

VFIIC

FVPH

H----

-Q

V-TL

CLLS

TNCV

LDPV

IYCF

L---

Plat

ypus

---VA

YSIIF

VLGI

FAN

GYVL

WVF

--FM

VNLT

VADL

LFLV

TMPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

-GIV

LSLV

IWVL

IVSS

ALYF

LV--

---IH

IFIV

ASFF

AVFL

LILV

CNL-

----

--RAL

WM

VCTV

LAVF

IICFV

PHH-

----

-V-T

LCLL

STN

CVLD

PVIY

CFLT

--Pu

fferfish

--PSA

YGVI

FILG

LLAN

LYVL

FI-

---M

TNLS

IADL

LFVC

ALPF

WIG

Y---

GSLF

FIN

TYCS

VLFL

GAIS

VN-G

IIISV

IIWVL

TISM

AIPY

LV--

-----F

AIIG

LFFV

VFAL

VLVC

NLL

I---

------

-LLA

VVGV

FVVC

FLPH

HVIQ

GAQ

I-TLL

LMGL

NCI

LDPV

VYCF

A---

Rabb

it--F

MVN

LTVA

DLLF

LITL

PLW

II---P

IVYS

IIFVL

GVVA

NGY

VLW

V-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--IAL

SLVI

WVS

ISAA

ATYF

LIL-

---IH

IFIV

ASFF

IVFL

IILIC

NL-

----

--RAL

WM

VCTV

LAVF

IICFV

PHH-

----

QV-

TLCL

LSTN

CVLD

PVIY

CFL-

--Ra

t--P

IVYS

VIFV

LGVV

ANGY

VLW

V---F

MVN

LTVA

DLLF

LMTL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

--G

ITLS

LVIW

ISIA

ATAS

YFLA

-----

VHIF

ITSC

FFLV

FFLI

FYCN

M--

-----R

ALW

MVC

TVLA

VFVI

CFVP

HH---

---I-

TLCL

LSTN

CVLD

PVIY

CFLT

--Sh

rew

-FPI

FYSI

IFVL

GVIA

NSY

VLW

----F

MVN

LTM

ADLL

FLIT

LPLW

IV-

NLT

GCFF

FIN

TYCS

VAFL

GVI--

--G

IYLS

LVIW

MAI

VSAA

SYFF

F--

---IH

IFLV

CSFF

LVFL

LIFF

CNL-

----

--RAL

WM

VCTV

LAVF

SICF

VPHH

-----

-V-T

LCLL

STN

CVLD

PIIY

CFLT

--Sq

uirr

elLF

PIIY

SIIF

VVGV

IAN

GYVL

-----F

MLN

LTVA

DLLF

LITL

PLW

II-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

---YL

SLVI

WVF

IVAA

ASYF

LILD

--VIH

IFIV

FSFF

LVFL

IILFC

K---

-----R

ALW

MVC

AVLA

VFVI

CFVP

HH---

--Q

V-TL

CLLS

TNCV

LDPV

IYCF

L---

Stickleba

ck-F

PVVY

SIIF

ILGI

TAN

LYVL

F--

CIYM

INLT

IGDL

LFVT

FLPF

W---

--SGS

LFFI

NTY

SSVL

FLCA

ISV-

-GIIL

SVFI

WLF

TLAT

AILF

LI--

----H

LTIV

IGFF

LVFF

VVVV

CNFL

----

-----Q

MLF

SVVG

VFVV

CFLP

HHAC

Q--

QI-T

WLL

MSL

NCI

LDPV

VYFF

A---

Zebrafi

sh-IY

MTN

LTVA

DLLF

VSAL

PFW

I----P

IFYG

VVFV

LGLI

ANCY

ALYV

----

GTFF

FIN

TYGS

ILFL

TVIS

INRG

AIIS

VLIW

AFIL

SASI

PQL-

-----

----L

RMLC

AVIG

VFVI

CFLP

HHVV

-----I

THFS

IIGLF

FLVF

LMVI

ICN

---Q

I-TLM

LMGI

NCL

LDPL

VYCF

A---


Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5097

99.9

0-H

GVGL

ILFG

VLSI

VVFD

ALLF

---F

IYGT

FCFA

CMM

CMGC

SSIY

H--

YYGI

SVLI

VASF

LPPL

YIGF

Y---

-ALY

MGC

ACVF

GGLC

LAM

AALP

--RI

MTY

VATV

MCG

LVPT

VHYV

F---

QTF

VGVF

LMFL

FYGM

GVLF

YC---

------

-IW

HVFV

LVAA

CV--H

FFTC

ISIY

-Hu

man

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

-----R

ALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

--Ch

icke

n--T

VFYS

IIFIL

GFVA

NCY

VLW

I---F

MLN

LTVA

DLLF

LVTM

PLW

IV-

SVAG

SLFF

VNTY

ASVA

FLM

VI---

-----R

ALW

MVC

TVLV

VFIIS

FVPH

H----

--G

IFLS

AAIW

IITVG

SALY

YIF-

-AI

HIIIC

TLFY

VIFL

FILT

WN

----V

TLCL

LSM

NCV

LDPV

IYCF

LT--

Chim

panz

ee-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

----

--RAL

WM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Cow

-FPI

FYSI

VFVL

GVIA

NSY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCFF

FIN

TYCS

VAFL

AVI--

---I

LLSL

IIWVS

IVGA

ASYF

FVL-

-IHIF

LVFS

FFLV

FLIIL

FCN

L--

-----R

ALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PIIY

CFLT

--El

epha

nt-F

PIFY

SIIF

VLGV

IAN

GYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

LFFI

NTY

CSVA

FLGV

I---

--ITL

SLVI

WVT

IVAA

ASYF

LFM

---H

IFIV

CSFF

LVFF

IILVC

NLV

----

--RAL

WM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--Fr

og---

VTYS

IIFVF

GFIA

NCY

VLW

IF-IF

MIN

LTIA

DLLF

LVTL

PLW

I--N

VAGC

FFFL

NTY

CSVA

FLGV

I---

--HFI

LVGA

FFVV

FLIIL

TCN

LV-

---CI

SVAV

WIL

LFAS

SLYF

LIFP

-----R

ALW

MVV

TVLA

VFVL

CFVP

HH---

-----

LCLM

STN

CMLD

PIIY

CFLT

KKGo

at-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

FFFI

NTY

CSVA

FLAV

I---

--FLL

SLIIW

VSIV

GAAS

YFFV

L--IH

IFLV

FSFF

LVFL

IILFC

NL-

----

--RAL

WM

VCTV

LAVF

VICF

VPHH

-----

-VTL

CLLS

TNCV

LDPI

IYCF

LT--

Guin

ea p

ig-F

PIVY

SIIF

VLGI

IAN

GYVL

W--

--FM

VNLT

VADL

LFLI

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--IAL

SLVI

WVA

IVAA

ASYF

LVM

--IH

ICIV

LGFF

IVFL

LILF

CNL-

----

--RAL

WM

VCTV

LAVF

VICF

VPHH

-----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--He

dgeh

og-F

PIFY

SIIF

ALGV

IAN

SYVL

W--

--FM

VNLT

LADL

LFLV

TLPL

WII-

NLA

GCFF

FIN

TYCS

VAFL

GVI--

--G

IILSL

VIW

VAIV

SAAS

YFFF

---IH

IFLV

VSFF

VVFL

IIFFC

NL-

----

--RAL

WM

VCTV

LAVF

IICFV

PHH-

----

QIT

LCLL

STN

CVLD

PIIY

CFL-

--Ho

rse

--PIV

YSIIF

VLGV

IAN

SYVL

WV-

-IFM

VNLT

VADL

LFLL

TLPL

WV-

-N

LTGC

CFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WLA

IVAA

ASYF

LFM

--IH

IFLV

FSFF

LVFL

IILVC

NL-

----

--RAL

WM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PIIY

CFL-

--M

edak

a-F

SVVY

SIFF

VVGL

LAN

VYVL

F--

--YM

TNLT

IADL

LFVC

ALPF

WIG

---T

GSFF

FIN

TYGT

ILFL

GAIS

L-RG

IIVSV

LIW

LCLV

SSAI

PSL-

----H

FLIIG

LFFV

VFFL

VMVC

NLL

----

-----Q

MLL

AVVG

VFLL

CFLP

HHVV

Q--

QIT

LVLM

GLN

CILD

PVIY

YFA-

--M

onke

y-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFFI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

----

--RAL

WM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--M

ouse

-FPI

VYSV

IFIL

GVVA

NGY

VLW

----F

MVN

LTM

ADLL

FLIT

LPLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

--G

ISLS

LIIW

VSIV

ATAS

YFLA

--VV

HVFI

AFCF

FLVF

FLIF

YCN

------

--RAL

WM

VCTV

LAVF

IICFV

PHH-

----

-ITLC

LLST

NCV

LDPV

IYCF

LT--

Opo

ssum

-FPI

VYSI

IFVL

GFVS

NAY

VLW

-----

MVN

LTVA

DLLF

LFAL

PLW

IVY

NVA

GCLF

FIN

TYCS

VCFL

GVI--

--G

ILLS

TIIW

VVIV

GCAS

YFLI

----H

VFIV

LCFF

IVFL

VILV

CNVI

----

----L

WM

VCTV

MAV

FFIC

FVPH

HVV-

--Q

VTLC

LMSI

NCV

LDPI

IYCF

L---

Ora

ngut

an-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

----

--RAL

WM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Pig

-FPI

FYSI

IFVL

GVIA

NSY

VLW

-----

MLN

LTM

ADLL

FLVT

LPLW

IIYN

LAGC

FFFI

NTY

CSVS

FLAV

I---

--ISL

SLIIW

VAM

VAAA

SYFF

VL-

-IHIF

LVFS

FFLV

FLVI

LFCN

L--

-----R

ALW

MVC

TVLA

VFIIC

FVPH

H----

--IT

LCLL

STN

CVLD

PIIY

CFLT

--Pi

ka-F

PIVY

SIIF

VLGV

VAN

GYVL

W--

--FM

VNLT

VADL

LFLI

TLPL

WII-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

IIWVV

ILAI

ASYF

LIM

--IH

IFIV

FCFF

VVFL

IILVC

NL-

----

--RAL

WM

VCTV

LGVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--Pl

atyp

us---

VAYS

IIFVL

GIFA

NGY

VLW

VF--F

MVN

LTVA

DLLF

LVTM

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

--G

IVLS

LVIW

VLIV

SSAL

YFLV

---IH

IFIV

ASFF

AVFL

LILV

CNL-

----

--RAL

WM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Puffe

rfish

--PSA

YGVI

FILG

LLAN

LYVL

FI-

---GS

LFFI

NTY

CSVL

FLGA

ISVN

---M

TNLS

IADL

LFVC

ALPF

WIG

Y-G

IIISV

IIWVL

TISM

AIPY

LV--

---FA

IIGLF

FVVF

ALVL

VCN

LLI

------

----L

LAVV

GVFV

VCFL

PHHV

IQGA

QIT

LLLM

GLN

CILD

PVVY

CFA-

--Ra

bbit

--PIV

YSIIF

VLGV

VAN

GYVL

WV-

--FM

VNLT

VADL

LFLI

TLPL

WII-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

ALSL

VIW

VSIS

AAAT

YFLI

L--IH

IFIV

ASFF

IVFL

IILIC

NL-

----

--RAL

WM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--Ra

t--P

IVYS

VIFV

LGVV

ANGY

VLW

V---F

MVN

LTVA

DLLF

LMTL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

--G

ITLS

LVIW

ISIA

ATAS

YFLA

---V

HIFI

TSCF

FLVF

FLIF

YCN

M--

-----R

ALW

MVC

TVLA

VFVI

CFVP

HH---

---IT

LCLL

STN

CVLD

PVIY

CFLT

--Sh

rew

-FPI

FYSI

IFVL

GVIA

NSY

VLW

----F

MVN

LTM

ADLL

FLIT

LPLW

IV-

NLT

GCFF

FIN

TYCS

VAFL

GVI--

--G

IYLS

LVIW

MAI

VSAA

SYFF

F--

-IHIF

LVCS

FFLV

FLLI

FFCN

L--

-----R

ALW

MVC

TVLA

VFSI

CFVP

HH---

---V

TLCL

LSTN

CVLD

PIIY

CFLT

--Sq

uirr

elLF

PIIY

SIIF

VVGV

IAN

GYVL

-----F

MLN

LTVA

DLLF

LITL

PLW

II-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

---YL

SLVI

WVF

IVAA

ASYF

LILD

VIHI

FIVF

SFFL

VFLI

ILFC

K---

-----R

ALW

MVC

AVLA

VFVI

CFVP

HH---

--Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Stickleba

ck-F

PVVY

SIIF

ILGI

TAN

LYVL

F--

CIYM

INLT

IGDL

LFVT

FLPF

W---

--SGS

LFFI

NTY

SSVL

FLCA

ISV-

-GIIL

SVFI

WLF

TLAT

AILF

LI--

--HLT

IVIG

FFLV

FFVV

VVCN

FL-

------

--QM

LFSV

VGVF

VVCF

LPHH

ACQ

--Q

ITW

LLM

SLN

CILD

PVVY

FFA-

--Zebrafi

sh-IY

MTN

LTVA

DLLF

VSAL

PFW

I----P

IFYG

VVFV

LGLI

ANCY

ALYV

----

GTFF

FIN

TYGS

ILFL

TVIS

INRG

AIIS

VLIW

AFIL

SASI

PQL-

-----

----L

RMLC

AVIG

VFVI

CFLP

HHVV

---IT

HFSI

IGLF

FLVF

LMVI

ICN

---Q

ITLM

LMGI

NCL

LDPL

VYCF

A---

Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5079

69.3

0---

AFLP

MM

AWLG

MW

YEVL

DMCV

L---

LLVF

LFPT

YTVA

CTM

ACIF

FTVV

AGIG

VAFL

IVLS

HMAF

LRV-

----E

VWHY

LLAF

FVM

HTLA

VSHA

V--

RFLG

ATLG

ALFG

GWVM

YTHY

G---

---FF

SWSF

LFQ

YVCC

LLVV

AIGK

--SY

FAVG

FMVF

YVCP

VILR

VFF-

--Hu

man

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

-LFF

INTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

--Ch

icke

n--T

VFYS

IIFIL

GFVA

NCY

VLW

I---F

MLN

LTVA

DLLF

LVTM

PLW

IV-

SVAG

S-LF

FVN

TYAS

VAFL

MVI

----G

IFLS

AAIW

IITVG

SALY

YIF-

-AI

HIIIC

TLFY

VIFL

FILT

WN

---RA

LWM

VCTV

LVVF

IISFV

PHH-

----

-VTL

CLLS

MN

CVLD

PVIY

CFLT

--Ch

impa

nzee

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

-LFF

INTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

--Co

w-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

-FFF

INTY

CSVA

FLAV

I---

--ILL

SLIIW

VSIV

GAAS

YFFV

L--IH

IFLV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPI

IYCF

LT--

Elep

hant

-FPI

FYSI

IFVL

GVIA

NGY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GC-L

FFIN

TYCS

VAFL

GVI--

---I

TLSL

VIW

VTIV

AAAS

YFLF

M-

--HIF

IVCS

FFLV

FFIIL

VCN

LV-

RALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Frog

---VT

YSIIF

VFGF

IAN

CYVL

WIF

-IFM

INLT

IADL

LFLV

TLPL

WI--

NVA

GC-F

FFLN

TYCS

VAFL

GVI--

----

CISV

AVW

ILLF

ASSL

YFLI

FP--H

FILV

GAFF

VVFL

IILTC

NLV

-RA

LWM

VVTV

LAVF

VLCF

VPHH

-----

---LC

LMST

NCM

LDPI

IYCF

LTKK

Goat

-FPI

FYSI

VFVL

GVIA

NSY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GC-F

FFIN

TYCS

VAFL

AVI--

--IH

IFLV

FSFF

LVFL

IILFC

NL-

---F

LLSL

IIWVS

IVGA

ASYF

FVL-

RALW

MVC

TVLA

VFVI

CFVP

HH---

---V

TLCL

LSTN

CVLD

PIIY

CFLT

--Gu

inea

pig

-FPI

VYSI

IFVL

GIIA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

IV-

NLA

GC-L

FFIN

TYCS

VAFL

GVI--

---I

ALSL

VIW

VAIV

AAAS

YFLV

M-

-IHIC

IVLG

FFIV

FLLI

LFCN

L--

RALW

MVC

TVLA

VFVI

CFVP

HH---

--Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Hedg

ehog

-FPI

FYSI

IFAL

GVIA

NSY

VLW

----F

MVN

LTLA

DLLF

LVTL

PLW

II-N

LAGC

-FFF

INTY

CSVA

FLGV

I---

-GIIL

SLVI

WVA

IVSA

ASYF

FF--

-IHIF

LVVS

FFVV

FLIIF

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

ITLC

LLST

NCV

LDPI

IYCF

L---

Hors

e--P

IVYS

IIFVL

GVIA

NSY

VLW

V--IF

MVN

LTVA

DLLF

LLTL

PLW

V--

NLT

GC-C

FFIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

LAIV

AAAS

YFLF

M-

-IHIF

LVFS

FFLV

FLIIL

VCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPI

IYCF

L---

Med

aka

-FSV

VYSI

FFVV

GLLA

NVY

VLF-

---Y

MTN

LTIA

DLLF

VCAL

PFW

IG-

--TGS

-FFF

INTY

GTIL

FLGA

ISL-

RGIIV

SVLI

WLC

LVSS

AIPS

L---

--HFL

IIGLF

FVVF

FLVM

VCN

LL-

---Q

MLL

AVVG

VFLL

CFLP

HHVV

Q--

QIT

LVLM

GLN

CILD

PVIY

YFA-

--M

onke

y-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NLA

GC-L

FFIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFFI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--M

ouse

-FPI

VYSV

IFIL

GVVA

NGY

VLW

----F

MVN

LTM

ADLL

FLIT

LPLW

IV-

NVA

GC-L

FFIN

TYCS

VAFL

GVI--

--G

ISLS

LIIW

VSIV

ATAS

YFLA

--VV

HVFI

AFCF

FLVF

FLIF

YCN

---RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-ITLC

LLST

NCV

LDPV

IYCF

LT--

Opo

ssum

-FPI

VYSI

IFVL

GFVS

NAY

VLW

-----

MVN

LTVA

DLLF

LFAL

PLW

IVY

NVA

GC-L

FFIN

TYCS

VCFL

GVI--

--G

ILLS

TIIW

VVIV

GCAS

YFLI

----H

VFIV

LCFF

IVFL

VILV

CNVI

---L

WM

VCTV

MAV

FFIC

FVPH

HVV-

--Q

VTLC

LMSI

NCV

LDPI

IYCF

L---

Ora

ngut

an-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GC-L

FFIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

--V

TLCL

LSTN

CVLD

PVIY

CFLT

--RA

LWM

VCTV

LAVF

IICFV

PHH-

----

Pig

-FPI

FYSI

IFVL

GVIA

NSY

VLW

-----

MLN

LTM

ADLL

FLVT

LPLW

IIYN

LAGC

-FFF

INTY

CSVS

FLAV

I---

--ISL

SLIIW

VAM

VAAA

SYFF

VL-

-IHIF

LVFS

FFLV

FLVI

LFCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--IT

LCLL

STN

CVLD

PIIY

CFLT

--Pi

ka-F

PIVY

SIIF

VLGV

VAN

GYVL

W--

--FM

VNLT

VADL

LFLI

TLPL

WII-

NVA

GC-L

FFIN

TYCS

VAFL

GVI--

---I

SLSL

IIWVV

ILAI

ASYF

LIM

--IH

IFIV

FCFF

VVFL

IILVC

NL-

-RA

LWM

VCTV

LGVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--Pl

atyp

us---

VAYS

IIFVL

GIFA

NGY

VLW

VF--F

MVN

LTVA

DLLF

LVTM

PLW

IV-

NVA

GC-L

FFIN

TYCS

VAFL

GVI--

--G

IVLS

LVIW

VLIV

SSAL

YFLV

---IH

IFIV

ASFF

AVFL

LILV

CNL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Puffe

rfish

--PSA

YGVI

FILG

LLAN

LYVL

FI-

---GS

-LFF

INTY

CSVL

FLGA

ISVN

---M

TNLS

IADL

LFVC

ALPF

WIG

Y-G

IIISV

IIWVL

TISM

AIPY

LV--

---FA

IIGLF

FVVF

ALVL

VCN

LLI

-----L

LAVV

GVFV

VCFL

PHHV

IQGA

QIT

LLLM

GLN

CILD

PVVY

CFA-

--Ra

bbit

--PIV

YSIIF

VLGV

VAN

GYVL

WV-

--FM

VNLT

VADL

LFLI

TLPL

WII-

NLA

GC-L

FFIN

TYCS

VAFL

GVI--

---I

ALSL

VIW

VSIS

AAAT

YFLI

L--IH

IFIV

ASFF

IVFL

IILIC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--Ra

t--P

IVYS

VIFV

LGVV

ANGY

VLW

V---F

MVN

LTVA

DLLF

LMTL

PLW

IV-

NLA

GC-L

FFIN

TYCS

VAFL

GVI--

--G

ITLS

LVIW

ISIA

ATAS

YFLA

---V

HIFI

TSCF

FLVF

FLIF

YCN

M--

RALW

MVC

TVLA

VFVI

CFVP

HH---

---IT

LCLL

STN

CVLD

PVIY

CFLT

--Sh

rew

-FPI

FYSI

IFVL

GVIA

NSY

VLW

----F

MVN

LTM

ADLL

FLIT

LPLW

IV-

NLT

GC-F

FFIN

TYCS

VAFL

GVI--

--G

IYLS

LVIW

MAI

VSAA

SYFF

F--

-IHIF

LVCS

FFLV

FLLI

FFCN

L--

RALW

MVC

TVLA

VFSI

CFVP

HH---

---V

TLCL

LSTN

CVLD

PIIY

CFLT

--Sq

uirr

elLF

PIIY

SIIF

VVGV

IAN

GYVL

-----F

MLN

LTVA

DLLF

LITL

PLW

II-N

VAGC

-LFF

INTY

CSVA

FLGV

I---

---YL

SLVI

WVF

IVAA

ASYF

LILD

VIHI

FIVF

SFFL

VFLI

ILFC

K---

RALW

MVC

AVLA

VFVI

CFVP

HH---

--Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Stickleba

ck-F

PVVY

SIIF

ILGI

TAN

LYVL

F--

CIYM

INLT

IGDL

LFVT

FLPF

W---

--SGS

-LFF

INTY

SSVL

FLCA

ISV-

-GIIL

SVFI

WLF

TLAT

AILF

LI--

--HLT

IVIG

FFLV

FFVV

VVCN

FL-

---Q

MLF

SVVG

VFVV

CFLP

HHAC

Q--

QIT

WLL

MSL

NCI

LDPV

VYFF

A---

Zebrafi

sh--P

IFYG

VVFV

LGLI

ANCY

ALYV

--IY

MTN

LTVA

DLLF

VSAL

PFW

I-----

GT-F

FFIN

TYGS

ILFL

TVIS

INRG

AIIS

VLIW

AFIL

SASI

PQL-

--IT

HFSI

IGLF

FLVF

LMVI

ICN

-----L

RMLC

AVIG

VFVI

CFLP

HHVV

---Q

ITLM

LMGI

NCL

LDPL

VYCF

A---


Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5110

01.5

0-G

SADV

GILL

CCM

VSVF

CRVK

V--

--PLT

QRV

MVG

VGW

LSVI

SNCL

T----

SQVL

YAFF

FCIC

DMVL

HVM

EG---

--RRA

LFIV

VLN

TAM

LFFL

VIFH

--YLA

CCGT

LY---

--VLG

CDVG

VLLQ

L---

-ALY

VTQ

LVFS

FGCS

ALYF

FML-

-GT

VGLG

LLDI

ARIL

LFGS

ILW---

---Hu

man

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

---IH

IFIV

FSFF

LVFL

IILFC

NL-

-----

RALW

MVC

TVLA

VFII-

CFVP

HH---

----V

TLCL

LSTN

CVLD

--PVI

YCFL

T--

Chic

ken

--TVF

YSIIF

ILGF

VAN

CYVL

WI-

--FM

LNLT

VADL

LFLV

TMPL

WIV

-SV

AGSL

FFVN

TYAS

VAFL

MVI

----G

IFLS

AAIW

IITVG

SALY

YIF-

---AI

HIIIC

TLFY

VIFL

FILT

WN

------

-RA

LWM

VCTV

LVVF

II-SF

VPHH

-----

--VTL

CLLS

MN

CVLD

--PVI

YCFL

T--

Chim

panz

ee-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L---

-IHIF

IVFS

FFLV

FLIIL

FCN

L----

--RA

LWM

VCTV

LAVF

II-CF

VPHH

-----

--VTL

CLLS

TNCV

LD--P

VIYC

FLT-

-Co

w-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

FFFI

NTY

CSVA

FLAV

I---

--ILL

SLIIW

VSIV

GAAS

YFFV

L---

-IHIF

LVFS

FFLV

FLIIL

FCN

L----

----V

TLCL

LSTN

CVLD

--PIIY

CFLT

--RA

LWM

VCTV

LAVF

II-CF

VPHH

-----

Elep

hant

-FPI

FYSI

IFVL

GVIA

NGY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

TLSL

VIW

VTIV

AAAS

YFLF

M---

--HIF

IVCS

FFLV

FFIIL

VCN

LV---

--RA

LWM

VCTV

LAVF

II-CF

VPHH

-----

-QVT

LCLL

STN

CVLD

--PVI

YCFL

---Fr

og---

VTYS

IIFVF

GFIA

NCY

VLW

IF-IF

MIN

LTIA

DLLF

LVTL

PLW

I--N

VAGC

FFFL

NTY

CSVA

FLGV

I---

---CI

SVAV

WIL

LFAS

SLYF

LIFP

----H

FILV

GAFF

VVFL

IILTC

NLV

-----

RALW

MVV

TVLA

VFVL

-CFV

PHH-

----

----L

CLM

STN

CMLD

--PIIY

CFLT

KKGo

at-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

NLA

GCFF

FIN

TYCS

VAFL

AVI--

----

MVN

LTM

ADLL

FLVT

LPLW

IVY

--FLL

SLIIW

VSIV

GAAS

YFFV

L---

-IHIF

LVFS

FFLV

FLIIL

FCN

L----

--RA

LWM

VCTV

LAVF

VI-C

FVPH

H----

---V

TLCL

LSTN

CVLD

--PIIY

CFLT

--Gu

inea

pig

-FPI

VYSI

IFVL

GIIA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

ALSL

VIW

VAIV

AAAS

YFLV

M---

-IHIC

IVLG

FFIV

FLLI

LFCN

L----

--RA

LWM

VCTV

LAVF

VI-C

FVPH

H----

--Q

VTLC

LLST

NCV

LD--P

VIYC

FL---

Hedg

ehog

-FPI

FYSI

IFAL

GVIA

NSY

VLW

----F

MVN

LTLA

DLLF

LVTL

PLW

II-N

LAGC

FFFI

NTY

CSVA

FLGV

I---

-GIIL

SLVI

WVA

IVSA

ASYF

FF---

--IH

IFLV

VSFF

VVFL

IIFFC

NL-

-----

RALW

MVC

TVLA

VFII-

CFVP

HH---

---Q

ITLC

LLST

NCV

LD--P

IIYCF

L---

Hors

e--P

IVYS

IIFVL

GVIA

NSY

VLW

V--IF

MVN

LTVA

DLLF

LLTL

PLW

V--

NLT

GCCF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

LAIV

AAAS

YFLF

M---

-IHIF

LVFS

FFLV

FLIIL

VCN

L----

--RA

LWM

VCTV

LAVF

II-CF

VPHH

-----

-QVT

LCLL

STN

CVLD

--PIIY

CFL-

--M

edak

a-F

SVVY

SIFF

VVGL

LAN

VYVL

F--

--YM

TNLT

IADL

LFVC

ALPF

WIG

---T

GSFF

FIN

TYGT

ILFL

GAIS

L-RG

IIVSV

LIW

LCLV

SSAI

PSL-

----

--HFL

IIGLF

FVVF

FLVM

VCN

LL---

-----

QM

LLAV

VGVF

LL-C

FLPH

HVVQ

---Q

ITLV

LMGL

NCI

LD--P

VIYY

FA---

Mon

key

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

FIL-

---IH

IFIV

FSFF

LVFL

IILFC

NL-

-----

RALW

MVC

TVLA

VFII-

CFVP

HH---

---Q

VTLC

LLST

NCV

LD--P

VIYC

FL---

Mou

se-F

PIVY

SVIF

ILGV

VAN

GYVL

W--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

-GIS

LSLI

IWVS

IVAT

ASYF

LA---

-VV

HVFI

AFCF

FLVF

FLIF

YCN

------

-RA

LWM

VCTV

LAVF

II-CF

VPHH

-----

--ITL

CLLS

TNCV

LD--P

VIYC

FLT-

-O

poss

um-F

PIVY

SIIF

VLGF

VSN

AYVL

W--

---M

VNLT

VADL

LFLF

ALPL

WIV

YN

VAGC

LFFI

NTY

CSVC

FLGV

I---

-GIL

LSTI

IWVV

IVGC

ASYF

LI---

---H

VFIV

LCFF

IVFL

VILV

CNVI

-----

--LW

MVC

TVM

AVFF

I-CFV

PHHV

V---

-QVT

LCLM

SIN

CVLD

--PIIY

CFL-

--O

rang

utan

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

---IH

IFIV

FSFF

LVFL

IILFC

NL-

-----

--VTL

CLLS

TNCV

LD--P

VIYC

FLT-

-RA

LWM

VCTV

LAVF

II-CF

VPHH

-----

Pig

-FPI

FYSI

IFVL

GVIA

NSY

VLW

-----

MLN

LTM

ADLL

FLVT

LPLW

IIYN

LAGC

FFFI

NTY

CSVS

FLAV

I---

-IHIF

LVFS

FFLV

FLVI

LFCN

L----

----I

SLSL

IIWVA

MVA

AASY

FFVL

---RA

LWM

VCTV

LAVF

II-CF

VPHH

-----

--ITL

CLLS

TNCV

LD--P

IIYCF

LT--

Pika

-FPI

VYSI

IFVL

GVVA

NGY

VLW

----F

MVN

LTVA

DLLF

LITL

PLW

II-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLIIW

VVIL

AIAS

YFLI

M---

-IHIF

IVFC

FFVV

FLIIL

VCN

L----

--RA

LWM

VCTV

LGVF

II-CF

VPHH

-----

-QVT

LCLL

STN

CVLD

--PVI

YCFL

---Pl

atyp

us---

VAYS

IIFVL

GIFA

NGY

VLW

VF--F

MVN

LTVA

DLLF

LVTM

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

--G

IVLS

LVIW

VLIV

SSAL

YFLV

----

-IHIF

IVAS

FFAV

FLLI

LVCN

L----

--RA

LWM

VCTV

LAVF

II-CF

VPHH

-----

--VTL

CLLS

TNCV

LD--P

VIYC

FLT-

-Pu

fferfish

--PSA

YGVI

FILG

LLAN

LYVL

FI-

---GS

LFFI

NTY

CSVL

FLGA

ISVN

---M

TNLS

IADL

LFVC

ALPF

WIG

Y-G

IIISV

IIWVL

TISM

AIPY

LV---

----

FAIIG

LFFV

VFAL

VLVC

NLL

I----

-----L

LAVV

GVFV

V-CF

LPHH

VIQ

GA-Q

ITLL

LMGL

NCI

LD--P

VVYC

FA---

Rabb

it--P

IVYS

IIFVL

GVVA

NGY

VLW

V---F

MVN

LTVA

DLLF

LITL

PLW

II-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--IAL

SLVI

WVS

ISAA

ATYF

LIL-

---IH

IFIV

ASFF

IVFL

IILIC

NL-

-----

RALW

MVC

TVLA

VFII-

CFVP

HH---

---Q

VTLC

LLST

NCV

LD--P

VIYC

FL---

Rat

--PIV

YSVI

FVLG

VVAN

GYVL

WV-

--FM

VNLT

VADL

LFLM

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

-GIT

LSLV

IWIS

IAAT

ASYF

LA---

--V

HIFI

TSCF

FLVF

FLIF

YCN

M---

---RA

LWM

VCTV

LAVF

VI-C

FVPH

H----

---I

TLCL

LSTN

CVLD

--PVI

YCFL

T--

Shre

w-F

PIFY

SIIF

VLGV

IAN

SYVL

W--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

-N

LTGC

FFFI

NTY

CSVA

FLGV

I---

-GIY

LSLV

IWM

AIVS

AASY

FFF-

----IH

IFLV

CSFF

LVFL

LIFF

CNL-

-----

RALW

MVC

TVLA

VFSI

-CFV

PHH-

----

--VTL

CLLS

TNCV

LD--P

IIYCF

LT--

Squi

rrel

LFPI

IYSI

IFVV

GVIA

NGY

VL---

--FM

LNLT

VADL

LFLI

TLPL

WII-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

----

YLSL

VIW

VFIV

AAAS

YFLI

LD--

VIHI

FIVF

SFFL

VFLI

ILFC

K----

---RA

LWM

VCAV

LAVF

VI-C

FVPH

H----

--Q

VTLC

LLST

NCV

LD--P

VIYC

FL---

Stickleba

ck-F

PVVY

SIIF

ILGI

TAN

LYVL

F--

CIYM

INLT

IGDL

LFVT

FLPF

W---

--SGS

LFFI

NTY

SSVL

FLCA

ISV-

-GIIL

SVFI

WLF

TLAT

AILF

LI---

---H

LTIV

IGFF

LVFF

VVVV

CNFL

-----

---Q

MLF

SVVG

VFVV

-CFL

PHHA

CQ--

-QIT

WLL

MSL

NCI

LD--P

VVYF

FA---

Zebrafi

sh-IY

MTN

LTVA

DLLF

VSAL

PFW

I----P

IFYG

VVFV

LGLI

ANCY

ALYV

----

GTFF

FIN

TYGS

ILFL

TVIS

INRG

AIIS

VLIW

AFIL

SASI

PQL-

----

--LRM

LCAV

IGVF

VI-C

FLPH

HVV-

--IT

HFSI

IGLF

FLVF

LMVI

ICN

------

--Q

ITLM

LMGI

NCL

LD--P

LVYC

FA---

Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5076

81.5

0-S

DLSV

TLLW

NLM

IGAV

KYVV

R--

------

----D

ARLL

WPV

PALV

VLGC

LAGT

KTT

AWCD

AFLL

VLVI

MCW

SSRV

---RA

FTLV

SGCL

ALLS

AALQ

WSF

------

LWSA

TLSI

QIL

QAL

IFIG

LHF

TGLF

VRCV

GSAA

SFLA

ALN

YC---

-----

TTFM

KLAV

VTH-

--FCF

MM

NEV

LIHu

man

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

------

--N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

-----

Chic

ken

--TVF

YSIIF

ILGF

VAN

CYVL

WI-

--FM

LNLT

VADL

LFLV

TMPL

WIV

------

--SV

AGSL

FFVN

TYAS

VAFL

MVI

----G

IFLS

AAIW

IITVG

SALY

YIF-

-AI

HIIIC

TLFY

VIFL

FILT

WN

---RA

LWM

VCTV

LVVF

IISFV

PHH-

----

-VTL

CLLS

MN

CVLD

PVIY

CFLT

-----

Chim

panz

ee-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV---

-----

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT---

--Co

w-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

Y----

---N

LAGC

FFFI

NTY

CSVA

FLAV

I---

--ILL

SLIIW

VSIV

GAAS

YFFV

L--IH

IFLV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPI

IYCF

LT---

--El

epha

nt-F

PIFY

SIIF

VLGV

IAN

GYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

Y----

---N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--ITL

SLVI

WVT

IVAA

ASYF

LFM

---H

IFIV

CSFF

LVFF

IILVC

NLV

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

-----

Frog

---VT

YSIIF

VFGF

IAN

CYVL

WIF

-IFM

INLT

IADL

LFLV

TLPL

WI--

------

-N

VAGC

FFFL

NTY

CSVA

FLGV

I---

--HFI

LVGA

FFVV

FLIIL

TCN

LV-

---CI

SVAV

WIL

LFAS

SLYF

LIFP

RALW

MVV

TVLA

VFVL

CFVP

HH---

-----

LCLM

STN

CMLD

PIIY

CFLT

KK---

Goat

-FPI

FYSI

VFVL

GVIA

NSY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY-

------

NLA

GCFF

FIN

TYCS

VAFL

AVI--

---F

LLSL

IIWVS

IVGA

ASYF

FVL-

-IHIF

LVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFVI

CFVP

HH---

---V

TLCL

LSTN

CVLD

PIIY

CFLT

-----

Guin

ea p

ig-F

PIVY

SIIF

VLGI

IAN

GYVL

W--

--FM

VNLT

VADL

LFLI

TLPL

WIV

------

--N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--IAL

SLVI

WVA

IVAA

ASYF

LVM

--IH

ICIV

LGFF

IVFL

LILF

CNL-

-RA

LWM

VCTV

LAVF

VICF

VPHH

-----

QVT

LCLL

STN

CVLD

PVIY

CFL-

-----

Hedg

ehog

-FPI

FYSI

IFAL

GVIA

NSY

VLW

----F

MVN

LTLA

DLLF

LVTL

PLW

II----

----

NLA

GCFF

FIN

TYCS

VAFL

GVI--

--G

IILSL

VIW

VAIV

SAAS

YFFF

---IH

IFLV

VSFF

VVFL

IIFFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

QIT

LCLL

STN

CVLD

PIIY

CFL-

-----

Hors

e--P

IVYS

IIFVL

GVIA

NSY

VLW

V--IF

MVN

LTVA

DLLF

LLTL

PLW

V----

-----

NLT

GCCF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

LAIV

AAAS

YFLF

M-

-IHIF

LVFS

FFLV

FLIIL

VCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPI

IYCF

L----

--M

edak

a-F

SVVY

SIFF

VVGL

LAN

VYVL

F--

--YM

TNLT

IADL

LFVC

ALPF

WIG

------

----T

GSFF

FIN

TYGT

ILFL

GAIS

L-RG

IIVSV

LIW

LCLV

SSAI

PSL-

----H

FLIIG

LFFV

VFFL

VMVC

NLL

----

QM

LLAV

VGVF

LLCF

LPHH

VVQ

--Q

ITLV

LMGL

NCI

LDPV

IYYF

A----

--M

onke

y-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV---

-----

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFFI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

-----

Mou

se-F

PIVY

SVIF

ILGV

VAN

GYVL

W--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

------

--N

VAGC

LFFI

NTY

CSVA

FLGV

I---

-GIS

LSLI

IWVS

IVAT

ASYF

LA--

VVHV

FIAF

CFFL

VFFL

IFYC

N---

RALW

MVC

TVLA

VFIIC

FVPH

H----

--IT

LCLL

STN

CVLD

PVIY

CFLT

-----

Opo

ssum

-FPI

VYSI

IFVL

GFVS

NAY

VLW

-----

MVN

LTVA

DLLF

LFAL

PLW

IVY-

------

NVA

GCLF

FIN

TYCS

VCFL

GVI--

--G

ILLS

TIIW

VVIV

GCAS

YFLI

----H

VFIV

LCFF

IVFL

VILV

CNVI

---L

WM

VCTV

MAV

FFIC

FVPH

HVV-

--Q

VTLC

LMSI

NCV

LDPI

IYCF

L----

--O

rang

utan

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

------

--N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

-----

Pig

-FPI

FYSI

IFVL

GVIA

NSY

VLW

-----

MLN

LTM

ADLL

FLVT

LPLW

IIY---

----

NLA

GCFF

FIN

TYCS

VSFL

AVI--

---I

SLSL

IIWVA

MVA

AASY

FFVL

--IH

IFLV

FSFF

LVFL

VILF

CNL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-ITLC

LLST

NCV

LDPI

IYCF

LT---

--Pi

ka-F

PIVY

SIIF

VLGV

VAN

GYVL

W--

--FM

VNLT

VADL

LFLI

TLPL

WII-

------

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLIIW

VVIL

AIAS

YFLI

M-

-IHIF

IVFC

FFVV

FLIIL

VCN

L--

RALW

MVC

TVLG

VFIIC

FVPH

H----

-Q

VTLC

LLST

NCV

LDPV

IYCF

L----

--Pl

atyp

us---

VAYS

IIFVL

GIFA

NGY

VLW

VF--F

MVN

LTVA

DLLF

LVTM

PLW

IV---

-----

NVA

GCLF

FIN

TYCS

VAFL

GVI--

--G

IVLS

LVIW

VLIV

SSAL

YFLV

---IH

IFIV

ASFF

AVFL

LILV

CNL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT---

--Pu

fferfish

--PSA

YGVI

FILG

LLAN

LYVL

FI-

---M

TNLS

IADL

LFVC

ALPF

WIG

Y----

------

GSLF

FIN

TYCS

VLFL

GAIS

VN-G

IIISV

IIWVL

TISM

AIPY

LV--

---FA

IIGLF

FVVF

ALVL

VCN

LLI

-----L

LAVV

GVFV

VCFL

PHHV

IQGA

QIT

LLLM

GLN

CILD

PVVY

CFA-

-----

Rabb

it--P

IVYS

IIFVL

GVVA

NGY

VLW

V---F

MVN

LTVA

DLLF

LITL

PLW

II----

----

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

ALSL

VIW

VSIS

AAAT

YFLI

L--IH

IFIV

ASFF

IVFL

IILIC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

-----

Rat

--PIV

YSVI

FVLG

VVAN

GYVL

WV-

--FM

VNLT

VADL

LFLM

TLPL

WIV

------

--N

LAGC

LFFI

NTY

CSVA

FLGV

I---

-GIT

LSLV

IWIS

IAAT

ASYF

LA--

-VHI

FITS

CFFL

VFFL

IFYC

NM

--RA

LWM

VCTV

LAVF

VICF

VPHH

-----

-ITLC

LLST

NCV

LDPV

IYCF

LT---

--Sh

rew

-FPI

FYSI

IFVL

GVIA

NSY

VLW

----F

MVN

LTM

ADLL

FLIT

LPLW

IV---

-----

NLT

GCFF

FIN

TYCS

VAFL

GVI--

--G

IYLS

LVIW

MAI

VSAA

SYFF

F--

-IHIF

LVCS

FFLV

FLLI

FFCN

L--

RALW

MVC

TVLA

VFSI

CFVP

HH---

---V

TLCL

LSTN

CVLD

PIIY

CFLT

-----

Squi

rrel

LFPI

IYSI

IFVV

GVIA

NGY

VL---

--FM

LNLT

VADL

LFLI

TLPL

WII-

------

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

---YL

SLVI

WVF

IVAA

ASYF

LILD

VIHI

FIVF

SFFL

VFLI

ILFC

K---

QVT

LCLL

STN

CVLD

PVIY

CFL-

-----

RALW

MVC

AVLA

VFVI

CFVP

HH---

--Sti

ckleba

ck-F

PVVY

SIIF

ILGI

TAN

LYVL

F--

CIYM

INLT

IGDL

LFVT

FLPF

W---

------

---S

GSLF

FIN

TYSS

VLFL

CAIS

V--G

IILSV

FIW

LFTL

ATAI

LFLI

----H

LTIV

IGFF

LVFF

VVVV

CNFL

----

QM

LFSV

VGVF

VVCF

LPHH

ACQ

--Q

ITW

LLM

SLN

CILD

PVVY

FFA-

-----

Zebrafi

sh--P

IFYG

VVFV

LGLI

ANCY

ALYV

--IY

MTN

LTVA

DLLF

VSAL

PFW

I-----

----

---GT

FFFI

NTY

GSIL

FLTV

ISIN

RGAI

ISVL

IWAF

ILSA

SIPQ

L---

ITHF

SIIG

LFFL

VFLM

VIIC

N---

--LRM

LCAV

IGVF

VICF

LPHH

VV---

QIT

LMLM

GIN

CLLD

PLVY

CFA-

-----


Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5068

25.2

4-F

NVW

SH---

---AL

AACF

VLYL

ALFP

PCT

CLVF

FFIF

ICSV

VYHL

FMP-

----

LSCD

VFGV

VICI

TGTS

WSL

VY---

VGM

GALL

LSTL

FVLY

GAVF

NA-

-----

FKAI

GFHS

VLRL

LILL

WVE

LP---

AVHC

HAM

SFFF

LALG

AVIN

AL--

GFW

HYCI

ILST

TMM

--LLG

CYYD

--Hu

man

-FPI

VYSI

IFVL

GVIA

NGY

VLW

------

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

---V

TLCL

LSTN

CVLD

PVIY

CFLT

--Ch

icke

n--T

VFYS

IIFIL

GFVA

NCY

VLW

I-----

----F

MLN

LTVA

DLLF

LVTM

PLW

IV-

SVAG

SLFF

VNTY

ASVA

FLM

VI---

-GIF

LSAA

IWIIT

VGSA

LYYI

F--

AIHI

IICTL

FYVI

FLFI

LTW

N---

RALW

MVC

TVLV

VFIIS

FVPH

H----

---V

TLCL

LSM

NCV

LDPV

IYCF

LT--

Chim

panz

ee-F

PIVY

SIIF

VLGV

IAN

GYVL

W---

------

-FM

VNLT

MAD

MLF

LITL

PLW

IV-

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

--IH

IFIV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

--VTL

CLLS

TNCV

LDPV

IYCF

LT--

Cow

-FPI

FYSI

VFVL

GVIA

NSY

VLW

------

-----M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

FFFI

NTY

CSVA

FLAV

I---

--ILL

SLIIW

VSIV

GAAS

YFFV

L--IH

IFLV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

--VTL

CLLS

TNCV

LDPI

IYCF

LT--

Elep

hant

-FPI

FYSI

IFVL

GVIA

NGY

VLW

------

-----M

VNLT

MAD

LLFL

VTLP

LWIV

Y--I

TLSL

VIW

VTIV

AAAS

YFLF

M-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---H

IFIV

CSFF

LVFF

IILVC

NLV

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-QVT

LCLL

STN

CVLD

PVIY

CFL-

--Fr

og---

VTYS

IIFVF

GFIA

NCY

VLW

IF---

----

IFM

INLT

IADL

LFLV

TLPL

WI--

NVA

GCFF

FLN

TYCS

VAFL

GVI--

----

CISV

AVW

ILLF

ASSL

YFLI

FP--H

FILV

GAFF

VVFL

IILTC

NLV

-RA

LWM

VVTV

LAVF

VLCF

VPHH

-----

----L

CLM

STN

CMLD

PIIY

CFLT

KKGo

at-F

PIFY

SIVF

VLGV

IAN

SYVL

W---

------

--MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCFF

FIN

TYCS

VAFL

AVI--

---F

LLSL

IIWVS

IVGA

ASYF

FVL-

-IHIF

LVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFVI

CFVP

HH---

----V

TLCL

LSTN

CVLD

PIIY

CFLT

--Gu

inea

pig

-FPI

VYSI

IFVL

GIIA

NGY

VLW

------

----F

MVN

LTVA

DLLF

LITL

PLW

IV-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

ALSL

VIW

VAIV

AAAS

YFLV

M-

-IHIC

IVLG

FFIV

FLLI

LFCN

L--

RALW

MVC

TVLA

VFVI

CFVP

HH---

---Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Hedg

ehog

-FPI

FYSI

IFAL

GVIA

NSY

VLW

------

----F

MVN

LTLA

DLLF

LVTL

PLW

II-N

LAGC

FFFI

NTY

CSVA

FLGV

I---

-GIIL

SLVI

WVA

IVSA

ASYF

FF--

-IHIF

LVVS

FFVV

FLIIF

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--Q

ITLC

LLST

NCV

LDPI

IYCF

L---

Hors

e--P

IVYS

IIFVL

GVIA

NSY

VLW

V----

----

IFM

VNLT

VADL

LFLL

TLPL

WV-

-N

LTGC

CFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WLA

IVAA

ASYF

LFM

--IH

IFLV

FSFF

LVFL

IILVC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-QVT

LCLL

STN

CVLD

PIIY

CFL-

--M

edak

a-F

SVVY

SIFF

VVGL

LAN

VYVL

F----

-----

-YM

TNLT

IADL

LFVC

ALPF

WIG

---T

GSFF

FIN

TYGT

ILFL

GAIS

L-RG

IIVSV

LIW

LCLV

SSAI

PSL-

----H

FLIIG

LFFV

VFFL

VMVC

NLL

----

QM

LLAV

VGVF

LLCF

LPHH

VVQ

---Q

ITLV

LMGL

NCI

LDPV

IYYF

A---

Mon

key

-FPI

VYSI

IFVL

GVIA

NGY

VLW

------

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

FIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Mou

se-F

PIVY

SVIF

ILGV

VAN

GYVL

W---

------

-FM

VNLT

MAD

LLFL

ITLP

LWIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

-GIS

LSLI

IWVS

IVAT

ASYF

LA--

VVHV

FIAF

CFFL

VFFL

IFYC

N---

RALW

MVC

TVLA

VFIIC

FVPH

H----

---I

TLCL

LSTN

CVLD

PVIY

CFLT

--O

poss

um-F

PIVY

SIIF

VLGF

VSN

AYVL

W---

------

--MVN

LTVA

DLLF

LFAL

PLW

IVY

NVA

GCLF

FIN

TYCS

VCFL

GVI--

--G

ILLS

TIIW

VVIV

GCAS

YFLI

----H

VFIV

LCFF

IVFL

VILV

CNVI

---L

WM

VCTV

MAV

FFIC

FVPH

HVV-

---Q

VTLC

LMSI

NCV

LDPI

IYCF

L---

Ora

ngut

an-F

PIVY

SIIF

VLGV

IAN

GYVL

W---

------

-FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

--VTL

CLLS

TNCV

LDPV

IYCF

LT--

Pig

-FPI

FYSI

IFVL

GVIA

NSY

VLW

------

-----M

LNLT

MAD

LLFL

VTLP

LWIIY

NLA

GCFF

FIN

TYCS

VSFL

AVI--

---I

SLSL

IIWVA

MVA

AASY

FFVL

--IH

IFLV

FSFF

LVFL

VILF

CNL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

--ITL

CLLS

TNCV

LDPI

IYCF

LT--

Pika

-FPI

VYSI

IFVL

GVVA

NGY

VLW

------

----F

MVN

LTVA

DLLF

LITL

PLW

II-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLIIW

VVIL

AIAS

YFLI

M-

-IHIF

IVFC

FFVV

FLIIL

VCN

L--

RALW

MVC

TVLG

VFIIC

FVPH

H----

--Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Plat

ypus

---VA

YSIIF

VLGI

FAN

GYVL

WVF

----

----F

MVN

LTVA

DLLF

LVTM

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

--IH

IFIV

ASFF

AVFL

LILV

CNL-

--G

IVLS

LVIW

VLIV

SSAL

YFLV

--RA

LWM

VCTV

LAVF

IICFV

PHH-

----

--VTL

CLLS

TNCV

LDPV

IYCF

LT--

Puffe

rfish

--PSA

YGVI

FILG

LLAN

LYVL

FI---

-----

--MTN

LSIA

DLLF

VCAL

PFW

IGY

---GS

LFFI

NTY

CSVL

FLGA

ISVN

-GIII

SVIIW

VLTI

SMAI

PYLV

-----

FAIIG

LFFV

VFAL

VLVC

NLL

I---

--LLA

VVGV

FVVC

FLPH

HVIQ

GA-Q

ITLL

LMGL

NCI

LDPV

VYCF

A---

Rabb

it--P

IVYS

IIFVL

GVVA

NGY

VLW

V----

----

-FM

VNLT

VADL

LFLI

TLPL

WII-

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

ALSL

VIW

VSIS

AAAT

YFLI

L--IH

IFIV

ASFF

IVFL

IILIC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-QVT

LCLL

STN

CVLD

PVIY

CFL-

--Ra

t--P

IVYS

VIFV

LGVV

ANGY

VLW

V----

----

-FM

VNLT

VADL

LFLM

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

-GIT

LSLV

IWIS

IAAT

ASYF

LA--

-VHI

FITS

CFFL

VFFL

IFYC

NM

--RA

LWM

VCTV

LAVF

VICF

VPHH

-----

--ITL

CLLS

TNCV

LDPV

IYCF

LT--

Shre

w-F

PIFY

SIIF

VLGV

IAN

SYVL

W---

------

-FM

VNLT

MAD

LLFL

ITLP

LWIV

-N

LTGC

FFFI

NTY

CSVA

FLGV

I---

-GIY

LSLV

IWM

AIVS

AASY

FFF-

--IH

IFLV

CSFF

LVFL

LIFF

CNL-

-RA

LWM

VCTV

LAVF

SICF

VPHH

-----

--VTL

CLLS

TNCV

LDPI

IYCF

LT--

Squi

rrel

LFPI

IYSI

IFVV

GVIA

NGY

VL---

----

----F

MLN

LTVA

DLLF

LITL

PLW

II-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

---YL

SLVI

WVF

IVAA

ASYF

LILD

VIHI

FIVF

SFFL

VFLI

ILFC

K---

RALW

MVC

AVLA

VFVI

CFVP

HH---

---Q

VTLC

LLST

NCV

LDPV

IYCF

L---

Stickleba

ck-F

PVVY

SIIF

ILGI

TAN

LYVL

F----

----C

IYM

INLT

IGDL

LFVT

FLPF

W---

--SGS

LFFI

NTY

SSVL

FLCA

ISV-

-GIIL

SVFI

WLF

TLAT

AILF

LI--

--HLT

IVIG

FFLV

FFVV

VVCN

FL-

---Q

MLF

SVVG

VFVV

CFLP

HHAC

Q--

-QIT

WLL

MSL

NCI

LDPV

VYFF

A---

Zebrafi

sh--P

IFYG

VVFV

LGLI

ANCY

ALYV

-----

---IY

MTN

LTVA

DLLF

VSAL

PFW

I-----

GTFF

FIN

TYGS

ILFL

TVIS

INRG

AIIS

VLIW

AFIL

SASI

PQL-

--IT

HFSI

IGLF

FLVF

LMVI

ICN

-----L

RMLC

AVIG

VFVI

CFLP

HHVV

----Q

ITLM

LMGI

NCL

LDPL

VYCF

A---

Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5115

07.6

0---

--SRV

VSVF

HSVL

VVPA

LLGG

VA-M

QG-

-LLC

ISIG

YFLY

DTAV

LVL

FHHV

VSSI

PYLI

------

YMFI

GY-Y

GLFI

LVC-

---FM

LVET

TNIS

-LF

FLW

IVFR

LIN

PTLL

LIIM

------

-AP

SVLC

AYVV

SFLC

YGVF

VIL-

-----

--SRV

VSVF

HSVL

VVPA

LLGG

VAHu

man

--FM

VNLT

MAD

MLF

LITL

PLW

IV---

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

--R

ALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

---Ch

icke

n--F

MLN

LTVA

DLLF

LVTM

PLW

IV---

SVAG

SLFF

VNTY

ASVA

FLM

VI---

-GIF

LSAA

IWIIT

VGSA

LYYI

F--

AIHI

IICTL

FYVI

FLFI

LTW

N---

-RAL

WM

VCTV

LVVF

IISFV

PHH-

----

-VTL

CLLS

MN

CVLD

PVIY

CFLT

----F

MLN

LTVA

DLLF

LVTM

PLW

IV---

Chim

panz

ee--F

MVN

LTM

ADM

LFLI

TLPL

WIV

---N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

-RAL

WM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

--FM

VNLT

MAD

MLF

LITL

PLW

IV---

Cow

---M

VNLT

MAD

LLFL

VTLP

LWIV

Y--

NLA

GCFF

FIN

TYCS

VAFL

AVI--

---I

LLSL

IIWVS

IVGA

ASYF

FVL-

-IHIF

LVFS

FFLV

FLIIL

FCN

L--

-RAL

WM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPI

IYCF

LT--

---M

VNLT

MAD

LLFL

VTLP

LWIV

Y--

Elep

hant

---M

VNLT

MAD

LLFL

VTLP

LWIV

Y--

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

TLSL

VIW

VTIV

AAAS

YFLF

M-

--HIF

IVCS

FFLV

FFIIL

VCN

LV-

-RAL

WM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY-

-Fr

og-IF

MIN

LTIA

DLLF

LVTL

PLW

I----

NVA

GCFF

FLN

TYCS

VAFL

GVI--

----

CISV

AVW

ILLF

ASSL

YFLI

FP--H

FILV

GAFF

VVFL

IILTC

NLV

--R

ALW

MVV

TVLA

VFVL

CFVP

HH---

-----

LCLM

STN

CMLD

PIIY

CFLT

KK-IF

MIN

LTIA

DLLF

LVTL

PLW

I----

Goat

---M

VNLT

MAD

LLFL

VTLP

LWIV

Y--

NLA

GCFF

FIN

TYCS

VAFL

AVI--

---F

LLSL

IIWVS

IVGA

ASYF

FVL-

-IHIF

LVFS

FFLV

FLIIL

FCN

L--

-RAL

WM

VCTV

LAVF

VICF

VPHH

-----

-VTL

CLLS

TNCV

LDPI

IYCF

LT--

---M

VNLT

MAD

LLFL

VTLP

LWIV

Y--

Guin

ea p

ig--F

MVN

LTVA

DLLF

LITL

PLW

IV---

NLA

GCLF

FIN

TYCS

VAFL

GVI--

---I

ALSL

VIW

VAIV

AAAS

YFLV

M-

-IHIC

IVLG

FFIV

FLLI

LFCN

L--

-RAL

WM

VCTV

LAVF

VICF

VPHH

-----

QVT

LCLL

STN

CVLD

PVIY

CFL-

----F

MVN

LTVA

DLLF

LITL

PLW

IV---

Hedg

ehog

--FM

VNLT

LADL

LFLV

TLPL

WII-

--N

LAGC

FFFI

NTY

CSVA

FLGV

I---

-GIIL

SLVI

WVA

IVSA

ASYF

FF--

-IHIF

LVVS

FFVV

FLIIF

FCN

L--

-RAL

WM

VCTV

LAVF

IICFV

PHH-

----

QIT

LCLL

STN

CVLD

PIIY

CFL-

----F

MVN

LTLA

DLLF

LVTL

PLW

II---

Hors

e-IF

MVN

LTVA

DLLF

LLTL

PLW

V----

NLT

GCCF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

LAIV

AAAS

YFLF

M-

-IHIF

LVFS

FFLV

FLIIL

VCN

L--

-RAL

WM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PIIY

CFL-

---IF

MVN

LTVA

DLLF

LLTL

PLW

V----

Med

aka

--YM

TNLT

IADL

LFVC

ALPF

WIG

-----T

GSFF

FIN

TYGT

ILFL

GAIS

L-RG

IIVSV

LIW

LCLV

SSAI

PSL-

----H

FLIIG

LFFV

VFFL

VMVC

NLL

----

-QM

LLAV

VGVF

LLCF

LPHH

VVQ

--Q

ITLV

LMGL

NCI

LDPV

IYYF

A---

--YM

TNLT

IADL

LFVC

ALPF

WIG

---M

onke

y--F

MVN

LTM

ADM

LFLI

TLPL

WIV

---N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

FIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

-RAL

WM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

---M

ouse

--FM

VNLT

MAD

LLFL

ITLP

LWIV

---N

VAGC

LFFI

NTY

CSVA

FLGV

I---

-GIS

LSLI

IWVS

IVAT

ASYF

LA--

VVHV

FIAF

CFFL

VFFL

IFYC

N---

-RAL

WM

VCTV

LAVF

IICFV

PHH-

----

-ITLC

LLST

NCV

LDPV

IYCF

LT--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

---O

poss

um---

MVN

LTVA

DLLF

LFAL

PLW

IVY-

-N

VAGC

LFFI

NTY

CSVC

FLGV

I---

-GIL

LSTI

IWVV

IVGC

ASYF

LI--

--HVF

IVLC

FFIV

FLVI

LVCN

VI-

---LW

MVC

TVM

AVFF

ICFV

PHHV

V---

QVT

LCLM

SIN

CVLD

PIIY

CFL-

-----

MVN

LTVA

DLLF

LFAL

PLW

IVY-

-O

rang

utan

--FM

VNLT

MAD

MLF

LITL

PLW

IV---

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

--R

ALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

---Pi

g---

MLN

LTM

ADLL

FLVT

LPLW

IIY--

NLA

GCFF

FIN

TYCS

VSFL

AVI--

---I

SLSL

IIWVA

MVA

AASY

FFVL

--IH

IFLV

FSFF

LVFL

VILF

CNL-

--R

ALW

MVC

TVLA

VFIIC

FVPH

H----

--IT

LCLL

STN

CVLD

PIIY

CFLT

-----

MLN

LTM

ADLL

FLVT

LPLW

IIY--

Pika

--FM

VNLT

VADL

LFLI

TLPL

WII-

--N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLIIW

VVIL

AIAS

YFLI

M-

-IHIF

IVFC

FFVV

FLIIL

VCN

L--

-RAL

WM

VCTV

LGVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

----F

MVN

LTVA

DLLF

LITL

PLW

II---

Plat

ypus

--FM

VNLT

VADL

LFLV

TMPL

WIV

---N

VAGC

LFFI

NTY

CSVA

FLGV

I---

-GIV

LSLV

IWVL

IVSS

ALYF

LV--

-IHIF

IVAS

FFAV

FLLI

LVCN

L--

-RAL

WM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

--FM

VNLT

VADL

LFLV

TMPL

WIV

---Pu

fferfish

---M

TNLS

IADL

LFVC

ALPF

WIG

Y--

---GS

LFFI

NTY

CSVL

FLGA

ISVN

-GIII

SVIIW

VLTI

SMAI

PYLV

-----

FAIIG

LFFV

VFAL

VLVC

NLL

I---

---LL

AVVG

VFVV

CFLP

HHVI

QGA

QIT

LLLM

GLN

CILD

PVVY

CFA-

-----

MTN

LSIA

DLLF

VCAL

PFW

IGY-

-Ra

bbit

--FM

VNLT

VADL

LFLI

TLPL

WII-

--N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--IAL

SLVI

WVS

ISAA

ATYF

LIL-

-IHIF

IVAS

FFIV

FLIIL

ICN

L--

-RAL

WM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

----F

MVN

LTVA

DLLF

LITL

PLW

II---

Rat

--FM

VNLT

VADL

LFLM

TLPL

WIV

---N

LAGC

LFFI

NTY

CSVA

FLGV

I---

-GIT

LSLV

IWIS

IAAT

ASYF

LA--

-VHI

FITS

CFFL

VFFL

IFYC

NM

---R

ALW

MVC

TVLA

VFVI

CFVP

HH---

---IT

LCLL

STN

CVLD

PVIY

CFLT

----F

MVN

LTVA

DLLF

LMTL

PLW

IV---

Shre

w--F

MVN

LTM

ADLL

FLIT

LPLW

IV---

NLT

GCFF

FIN

TYCS

VAFL

GVI--

--G

IYLS

LVIW

MAI

VSAA

SYFF

F--

-IHIF

LVCS

FFLV

FLLI

FFCN

L--

-RAL

WM

VCTV

LAVF

SICF

VPHH

-----

-VTL

CLLS

TNCV

LDPI

IYCF

LT--

--FM

VNLT

MAD

LLFL

ITLP

LWIV

---Sq

uirr

el--F

MLN

LTVA

DLLF

LITL

PLW

II---

NVA

GCLF

FIN

TYCS

VAFL

GVI--

----

YLSL

VIW

VFIV

AAAS

YFLI

LDVI

HIFI

VFSF

FLVF

LIIL

FCK-

---R

ALW

MVC

AVLA

VFVI

CFVP

HH---

--Q

VTLC

LLST

NCV

LDPV

IYCF

L---

--FM

LNLT

VADL

LFLI

TLPL

WII-

--Sti

ckleba

ckCI

YMIN

LTIG

DLLF

VTFL

PFW

-----

--SGS

LFFI

NTY

SSVL

FLCA

ISV-

-GIIL

SVFI

WLF

TLAT

AILF

LI--

--HLT

IVIG

FFLV

FFVV

VVCN

FL-

----Q

MLF

SVVG

VFVV

CFLP

HHAC

Q--

QIT

WLL

MSL

NCI

LDPV

VYFF

A---

CIYM

INLT

IGDL

LFVT

FLPF

W---

--Zebrafi

sh-IY

MTN

LTVA

DLLF

VSAL

PFW

I----

---GT

FFFI

NTY

GSIL

FLTV

ISIN

RGAI

ISVL

IWAF

ILSA

SIPQ

L---

ITHF

SIIG

LFFL

VFLM

VIIC

N---

---LR

MLC

AVIG

VFVI

CFLP

HHVV

---Q

ITLM

LMGI

NCL

LDPL

VYCF

A---

-IYM

TNLT

VADL

LFVS

ALPF

WI--

--


Sequ

ence

Dom

ain

ID

omai

n II

Dom

ain

III

Dom

ain

IVD

omai

n V

Dom

ain

VI

Dom

ain

VII

Tc00

.104

7053

5085

35.3

0-C

HMAD

LVIW

VYIL

ALLL

FMSH

-----

-LHY

FLLD

YCYF

HNAC

-----

------

-----L

VLFL

LWCL

VDVQ

---FL

SFFA

LVAG

SFGP

ILGA

ILV-S

YWTM

LKLH

FVM

FIAW

QIF

YH--

MFG

TGYL

PTVI

LFQ

VAQ

WLL

H----

-AL

SAW

PLAL

YLIF

FLLY

TVYN

---Hu

man

-FPI

VYSI

IFVL

GVIA

NGY

VLW

----F

MVN

LTM

ADM

LFLI

TLPL

WIV

-N

VAGC

LFFI

NTY

CSVA

FLGV

I---

--ISL

SLVI

WVA

IVGA

ASYF

LIL-

-IHIF

IVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PVIY

CFLT

--Ch

icke

n--T

VFYS

IIFIL

GFVA

NCY

VLW

I---F

MLN

LTVA

DLLF

LVTM

PLW

IV-

SVAG

SLFF

VNTY

ASVA

FLM

VI---

-GIF

LSAA

IWIIT

VGSA

LYYI

F--

AIHI

IICTL

FYVI

FLFI

LTW

N---

RALW

MVC

TVLV

VFIIS

FVPH

H----

--V

TLCL

LSM

NCV

LDPV

IYCF

LT--

Chim

panz

ee-F

PIVY

SIIF

VLGV

IAN

GYVL

W--

--FM

VNLT

MAD

MLF

LITL

PLW

IV-

NVA

GCLF

FIN

TYCS

VAFL

GVI--

---I

SLSL

VIW

VAIV

GAAS

YFLI

L--IH

IFIV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

-VTL

CLLS

TNCV

LDPV

IYCF

LT--

Cow

-FPI

FYSI

VFVL

GVIA

NSY

VLW

-----

MVN

LTM

ADLL

FLVT

LPLW

IVY

NLA

GCFF

FIN

TYCS

VAFL

AVI--

---I

LLSL

IIWVS

IVGA

ASYF

FVL-

-IHIF

LVFS

FFLV

FLIIL

FCN

L--

RALW

MVC

TVLA

VFIIC

FVPH

H----

--V

TLCL

LSTN

CVLD

PIIY

CFLT

--El

epha

nt-F

PIFY

SIIF

VLGV

IAN

GYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

LFFI

NTY

CSVA

FLGV

I---

--ITL

SLVI

WVT

IVAA

ASYF

LFM

---H

IFIV

CSFF

LVFF

IILVC

NLV

-RA

LWM

VCTV

LAVF

IICFV

PHH-

----

QVT

LCLL

STN

CVLD

PVIY

CFL-

--Fr

og---

VTYS

IIFVF

GFIA

NCY

VLW

IF-IF

MIN

LTIA

DLLF

LVTL

PLW

I--N

VAGC

FFFL

NTY

CSVA

FLGV

I---

---CI

SVAV

WIL

LFAS

SLYF

LIFP

--HFI

LVGA

FFVV

FLIIL

TCN

LV-

RALW

MVV

TVLA

VFVL

CFVP

HH---

-----

LCLM

STN

CMLD

PIIY

CFLT

KKGo

at-F

PIFY

SIVF

VLGV

IAN

SYVL

W--

---M

VNLT

MAD

LLFL

VTLP

LWIV

YN

LAGC

FFFI

NTY

CSVA

FLAV

I---

--FLL

SLIIW

VSIV

GAAS

YFFV

L--IH

IFLV

FSFF

LVFL

IILFC

NL-

-RA

LWM

VCTV

LAVF

VICF

VPHH

-----

-VTL

CLLS

TNCV

LDPI

IYCF

LT--

Guin

ea p

ig-F

PIVY

SIIF

VLGI

IAN

GYVL

W--

--FM

VNLT

VADL

LFLI

TLPL

WIV

-N

LAGC

LFFI

NTY

CSVA

FLGV

I---

--IAL

SLVI

WVA

IVAA

ASYF

LVM

--IH

ICIV

LGFF

IVFL

LILF

CNL-

-RA

LWM

VCTV

LAVF

VICF

VPHH

-----

QVT

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Res

cale

d co

nser

vatio

n sc

ores

of t

he a

min

o ac

ids f

rom

tran

smem

bran

e do

mai

ns fr

om T

rypa

noso

ma

cruz

i hyp

othe

tical

pro

tein

s: D

omai

n I

Tc00

. 10

47

0535

08

21

5.6

Tc00

. 10

47

0535

08

72

3.24

Tc00

. 10

47

0535

09

79

1.60

Tc00

. 10

47

0535

06

75

3.30

Tc00

. 10

47

0535

05

19

3.74

Tc00

. 10

47

0535

03

46

3.20

Tc00

. 10

47

0535

04

13

7.150

Tc00

. 10

47

0535

06

46

7.4

Tc00

. 10

47

0535

09

79

9.100

Tc00

. 10

47

0535

08

64

1.20

0

Tc00

. 10

47

0535

11

78

7.30

Tc00

. 10

47

0535

10

30

5.79

Tc00

. 10

47

0535

10

86

1.94

Tc00

. 10

47

0535

11

92

3.71

Tc00

. 10

47

0535

11

92

1.71

Tc00

. 10

47

0535

03

73

5.30

Tc00

. 10

47

0535

10

35

5.19

0

Tc00

. 10

47

0535

09

71

9.56

Tc00

. 10

47

0535

08

31

7.70

Tc00

. 10

47

0535

09

84

9.30

Tc00

. 10

47

0535

10

72

9.29

0

Tc00

. 10

47

0535

04

13

7.140

Tc00

. 10

47

0535

09

79

9.90

Tc00

. 10

47

0535

07

96

9.30

Tc00

. 10

47

0535

11

00

1.50

Tc00

. 10

47

0535

07

68

1.50

Tc00

. 10

47

0535

06

82

5.24

Tc00

. 10

47

0535

11

50

7.60

Tc00

. 10

47

0535

08

53

5.30

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9543

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911

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1315

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Res

cale

d co

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vatio

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of t

he a

min

o ac

ids f

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tran

smem

bran

e do

mai

ns fr

om T

rypa

noso

ma

cruz

i hyp

othe

tical

pro

tein

s: D

omai

n II

Tc00

. 10

47

0535

08

21

5.6

Tc00

. 10

47

0535

08

72

3.24

Tc00

. 10

47

0535

09

79

1.60

Tc00

. 10

47

0535

06

75

3.30

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. 10

47

0535

05

19

3.74

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. 10

47

0535

03

46

3.20

Tc00

. 10

47

0535

04

13

7.150

Tc00

. 10

47

0535

06

46

7.4

Tc00

. 10

47

0535

09

79

9.100

Tc00

. 10

47

0535

08

64

1.20

0

Tc00

. 10

47

0535

11

78

7.30

Tc00

. 10

47

0535

10

30

5.79

Tc00

. 10

47

0535

10

86

1.94

Tc00

. 10

47

0535

11

92

3.71

Tc00

. 10

47

0535

11

92

1.71

Tc00

. 10

47

0535

03

73

5.30

Tc00

. 10

47

0535

10

35

5.19

0

Tc00

. 10

47

0535

09

71

9.56

Tc00

. 10

47

0535

08

31

7.70

Tc00

. 10

47

0535

09

84

9.30

Tc00

. 10

47

0535

10

72

9.29

0

Tc00

. 10

47

0535

04

13

7.140

Tc00

. 10

47

0535

09

79

9.90

Tc00

. 10

47

0535

07

96

9.30

Tc00

. 10

47

0535

11

00

1.50

Tc00

. 10

47

0535

07

68

1.50

Tc00

. 10

47

0535

06

82

5.24

Tc00

. 10

47

0535

11

50

7.60

Tc00

. 10

47

0535

08

53

5.30

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097

--

9743

2743

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3330

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177

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67


Res

cale

d co

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vatio

n sc

ores

of t

he a

min

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tran

smem

bran

e do

mai

ns fr

om T

rypa

noso

ma

cruz

i hyp

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tical

pro

tein

s: D

omai

n II

I

Tc00

. 10

47

0535

08

21

5.6

Tc00

. 10

47

0535

08

72

3.24

Tc00

. 10

47

0535

09

79

1.60

Tc00

. 10

47

0535

06

75

3.30

Tc00

. 10

47

0535

05

19

3.74

Tc00

. 10

47

0535

03

46

3.20

Tc00

. 10

47

0535

04

13

7.150

Tc00

. 10

47

0535

06

46

7.4

Tc00

. 10

47

0535

09

79

9.100

Tc00

. 10

47

0535

08

64

1.20

0

Tc00

. 10

47

0535

11

78

7.30

Tc00

. 10

47

0535

10

30

5.79

Tc00

. 10

47

0535

10

86

1.94

Tc00

. 10

47

0535

11

92

3.71

Tc00

. 10

47

0535

11

92

1.71

Tc00

. 10

47

0535

03

73

5.30

Tc00

. 10

47

0535

10

35

5.19

0

Tc00

. 10

47

0535

09

71

9.56

Tc00

. 10

47

0535

08

31

7.70

Tc00

. 10

47

0535

09

84

9.30

Tc00

. 10

47

0535

10

72

9.29

0

Tc00

. 10

47

0535

04

13

7.140

Tc00

. 10

47

0535

09

79

9.90

Tc00

. 10

47

0535

07

96

9.30

Tc00

. 10

47

0535

11

00

1.50

Tc00

. 10

47

0535

07

68

1.50

Tc00

. 10

47

0535

06

82

5.24

Tc00

. 10

47

0535

11

50

7.60

Tc00

. 10

47

0535

08

53

5.30

--

--

--

65-

65-

84-

--

--

--

9984

-67

6750

-96

310

--

--

--

-40

-40

-99

--

-94

--

-41

0-

1111

78-

2415

63-

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34-

99-

3399

3316

33-

--

44-

--

440

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3387

-95

1617

--

9999

-0

-8

08

3499

-9

1299

--

9941

3512

88

9930

1331

99-

9929

29-

2999

3629

361

0-

3519

1634

-5

1769

1369

6920

096

9-

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8585

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9985

9885

8547

-47

497

47-

9829

498

5050

5447

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--

240

4032

492

5049

5099

45-

1448

2212

4699

5317

6750

5029

4518

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1919

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1999

6330

4545

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9964

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9956

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980

3059

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1942

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3972

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8199

3998

8686

730

1926

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711

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034

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029

160

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536

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9934

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160

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4318

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9960

4362

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9999

9999

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5918

5932

998

5657

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550

9933

9959

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108

355

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4220

4446

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420

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04

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2934

4847

4648

4631

3699

146

370

4737

4746

5495

9538

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947

3157

9696

5497

5723

9723

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9932

9732

3156

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2323

920

797

5634

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1933

3735

2237

2252

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5099

205

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8-

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43


Res

cale

d co

nser

vatio

n sc

ores

of t

he a

min

o ac

ids f

rom

tran

smem

bran

e do

mai

ns fr

om T

rypa

noso

ma

cruz

i hyp

othe

tical

pro

tein

s: D

omai

n IV

Tc00

. 10

47

0535

08

21

5.6

Tc00

. 10

47

0535

08

72

3.24

Tc00

. 10

47

0535

09

79

1.60

Tc00

. 10

47

0535

06

75

3.30

Tc00

. 10

47

0535

05

19

3.74

Tc00

. 10

47

0535

03

46

3.20

Tc00

. 10

47

0535

04

13

7.150

Tc00

. 10

47

0535

06

46

7.4

Tc00

. 10

47

0535

09

79

9.100

Tc00

. 10

47

0535

08

64

1.20

0

Tc00

. 10

47

0535

11

78

7.30

Tc00

. 10

47

0535

10

30

5.79

Tc00

. 10

47

0535

10

86

1.94

Tc00

. 10

47

0535

11

92

3.71

Tc00

. 10

47

0535

11

92

1.71

Tc00

. 10

47

0535

03

73

5.30

Tc00

. 10

47

0535

10

35

5.19

0

Tc00

. 10

47

0535

09

71

9.56

Tc00

. 10

47

0535

08

31

7.70

Tc00

. 10

47

0535

09

84

9.30

Tc00

. 10

47

0535

10

72

9.29

0

Tc00

. 10

47

0535

04

13

7.140

Tc00

. 10

47

0535

09

79

9.90

Tc00

. 10

47

0535

07

96

9.30

Tc00

. 10

47

0535

11

00

1.50

Tc00

. 10

47

0535

07

68

1.50

Tc00

. 10

47

0535

06

82

5.24

Tc00

. 10

47

0535

11

50

7.60

Tc00

. 10

47

0535

08

53

5.30

-40

4050

--

45-

4599

2937

9737

5397

5047

380

2247

4761

-36

5412

-86

7777

57-

8679

-79

9994

9486

8647

8657

8494

099

7676

39-

9655

9986

9740

4025

-97

96-

9699

323

973

097

2599

5939

052

529

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2250

9729

9999

30-

2999

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2226

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2623

2830

526

025

2224

128

82

9999

4497

9773

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58-

580

9898

9773

1697

7341

9898

735

698

417

9914

4319

1818

8199

1981

9981

4358

432

440

3281

5851

8283

4343

6043

160

8333

9999

9950

3399

2232

2256

024

358

563

5036

587

462

526

385

161

2621

66

3099

2132

9932

2125

1921

422

2130

996

244

02

2723

165

2620

7036

3629

2070

3020

3023

5699

5771

057

2970

5724

5623

2427

7036

5755

9875

00

3471

7524

7124

1971

7171

4558

7134

1999

7173

1919

7373

7158

6599

1258

5898

5012

5950

5926

3053

2321

5223

980

5399

490

256

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58-

528

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5921

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2038

1799

698

570

9859

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5734

5656

357

1957

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32-

-37

4932

8449

8499

8284

4433

9944

37-

099

8231

3254

4199

6-

119

--

628

1938

2838

9933

420

590

206

-19

1662

1617

2058

9835

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38-

-97

3038

2714

2798

898

230

2723

97-

36-

3799

9897

9820

98-

3995

--

9529

9517

2917

3314

3296

2099

9695

--

--

3030

3330

033

-52

796

9617

167

2215

22-

03

9618

6196

1798

5553

-0

119

2014

6399

2244

7373

1739

4496

3996

-73

1432

9917

3217

7344

9798

1011

3997

2416

260

4398

9813

1743

6017

60-

498

-98

0-

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4598

4199

9911

5756

955

1155

2727

5724

558

248

-15

--

-21

-57

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8413

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8555

--

9994

5621

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--

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--

--

-62

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52-

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99


Res

cale

d co

nser

vatio

n sc

ores

of t

he a

min

o ac

ids f

rom

tran

smem

bran

e do

mai

ns fr

om T

rypa

noso

ma

cruz

i hyp

othe

tical

pro

tein

s: D

omai

n V

Tc00

. 10

47

0535

08

21

5.6

Tc00

. 10

47

0535

08

72

3.24

Tc00

. 10

47

0535

09

79

1.60

Tc00

. 10

47

0535

06

75

3.30

Tc00

. 10

47

0535

05

19

3.74

Tc00

. 10

47

0535

03

46

3.20

Tc00

. 10

47

0535

04

13

7.150

Tc00

. 10

47

0535

06

46

7.4

Tc00

. 10

47

0535

09

79

9.100

Tc00

. 10

47

0535

08

64

1.20

0

Tc00

. 10

47

0535

11

78

7.30

Tc00

. 10

47

0535

10

30

5.79

Tc00

. 10

47

0535

10

86

1.94

Tc00

. 10

47

0535

11

92

3.71

Tc00

. 10

47

0535

11

92

1.71

Tc00

. 10

47

0535

03

73

5.30

Tc00

. 10

47

0535

10

35

5.19

0

Tc00

. 10

47

0535

09

71

9.56

Tc00

. 10

47

0535

08

31

7.70

Tc00

. 10

47

0535

09

84

9.30

Tc00

. 10

47

0535

10

72

9.29

0

Tc00

. 10

47

0535

04

13

7.140

Tc00

. 10

47

0535

09

79

9.90

Tc00

. 10

47

0535

07

96

9.30

Tc00

. 10

47

0535

11

00

1.50

Tc00

. 10

47

0535

07

68

1.50

Tc00

. 10

47

0535

06

82

5.24

Tc00

. 10

47

0535

11

50

7.60

Tc00

. 10

47

0535

08

53

5.30

23-

--

6199

1861

1835

34-

3635

1436

61-

--

1634

9918

--

-0

1450

--

-50

078

5078

7455

-56

6365

5699

--

-58

7654

5376

--

5678

66-

-37

160

7616

7621

9976

6767

5067

6728

--

1675

4514

6566

6767

229

--

1524

9915

2415

1017

3710

2121

1037

8-

-11

924

109

70

3713

9862

6263

9899

5698

5664

3310

554

1755

5959

--

3230

5829

014

2698

5537

66

9631

3796

3196

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9694

1435

34-

-98

9931

330

3067

3536

9961

6134

6192

5461

5494

5413

5861

5458

4756

6161

3445

5454

5456

5658

023

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2092

2392

2313

2330

4189

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282

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3223

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9746

6282

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3936

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1030

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0-

5538

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439

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00

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6243

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8778

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1273

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746

5817

1726

1057

9510

9520

230

6054

2860

539

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5251

1622

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972

145

7878

6678

868

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9797

6699

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310

9966

5278

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1837

9898

15-

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995

3718

270

2626

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--

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--

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17


Res

cale

d co

nser

vatio

n sc

ores

of t

he a

min

o ac

ids f

rom

tran

smem

bran

e do

mai

ns fr

om T

rypa

noso

ma

cruz

i hyp

othe

tical

pro

tein

s: D

omai

n V

I

Tc00

. 10

47

0535

08

21

5.6

Tc00

. 10

47

0535

08

72

3.24

Tc00

. 10

47

0535

09

79

1.60

Tc00

. 10

47

0535

06

75

3.30

Tc00

. 10

47

0535

05

19

3.74

Tc00

. 10

47

0535

03

46

3.20

Tc00

. 10

47

0535

04

13

7.150

Tc00

. 10

47

0535

06

46

7.4

Tc00

. 10

47

0535

09

79

9.100

Tc00

. 10

47

0535

08

64

1.20

0

Tc00

. 10

47

0535

11

78

7.30

Tc00

. 10

47

0535

10

30

5.79

Tc00

. 10

47

0535

10

86

1.94

Tc00

. 10

47

0535

11

92

3.71

Tc00

. 10

47

0535

11

92

1.71

Tc00

. 10

47

0535

03

73

5.30

Tc00

. 10

47

0535

10

35

5.19

0

Tc00

. 10

47

0535

09

71

9.56

Tc00

. 10

47

0535

08

31

7.70

Tc00

. 10

47

0535

09

84

9.30

Tc00

. 10

47

0535

10

72

9.29

0

Tc00

. 10

47

0535

04

13

7.140

Tc00

. 10

47

0535

09

79

9.90

Tc00

. 10

47

0535

07

96

9.30

Tc00

. 10

47

0535

11

00

1.50

Tc00

. 10

47

0535

07

68

1.50

Tc00

. 10

47

0535

06

82

5.24

Tc00

. 10

47

0535

11

50

7.60

Tc00

. 10

47

0535

08

53

5.30

--

--

21-

2721

2820

1328

1322

9913

-44

--

-8

8-

-20

-0

16-

--

-74

-96

7499

2345

9993

8245

93-

54-

--

33

--

91-

06

--

--

46-

9046

9215

8821

393

9539

-97

4040

-99

99-

-88

-91

0-

--

-14

-14

1414

140

011

9994

11-

125

5-

55

16-

153

903

--

--

40-

4840

4849

4212

4844

2148

-46

9999

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2124

1740

3947

015

2626

079

1554

7954

7995

7996

780

961

7829

2999

7878

1779

64

968

040

4012

110

1611

1640

2929

1099

9410

1312

9191

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4289

9639

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029

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9999

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280

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6317

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027

276

160

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279

99-

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71

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2018

277

1951

1515

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2330

9727

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2736

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--

594

992

-16

055

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99


Res

cale

d co

nser

vatio

n sc

ores

of t

he a

min

o ac

ids f

rom

tran

smem

bran

e do

mai

ns fr

om T

rypa

noso

ma

cruz

i hyp

othe

tical

pro

tein

s: D

omai

n V

II

Tc00

. 10

47

0535

08

21

5.6

Tc00

. 10

47

0535

08

72

3.24

Tc00

. 10

47

0535

09

79

1.60

Tc00

. 10

47

0535

06

75

3.30

Tc00

. 10

47

0535

05

19

3.74

Tc00

. 10

47

0535

03

46

3.20

Tc00

. 10

47

0535

04

13

7.150

Tc00

. 10

47

0535

06

46

7.4

Tc00

. 10

47

0535

09

79

9.100

Tc00

. 10

47

0535

08

64

1.20

0

Tc00

. 10

47

0535

11

78

7.30

Tc00

. 10

47

0535

10

30

5.79

Tc00

. 10

47

0535

10

86

1.94

Tc00

. 10

47

0535

11

92

3.71

Tc00

. 10

47

0535

11

92

1.71

Tc00

. 10

47

0535

03

73

5.30

Tc00

. 10

47

0535

10

35

5.19

0

Tc00

. 10

47

0535

09

71

9.56

Tc00

. 10

47

0535

08

31

7.70

Tc00

. 10

47

0535

09

84

9.30

Tc00

. 10

47

0535

10

72

9.29

0

Tc00

. 10

47

0535

04

13

7.140

Tc00

. 10

47

0535

09

79

9.90

Tc00

. 10

47

0535

07

96

9.30

Tc00

. 10

47

0535

11

00

1.50

Tc00

. 10

47

0535

07

68

1.50

Tc00

. 10

47

0535

06

82

5.24

Tc00

. 10

47

0535

11

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search for a platelet-activating factor receptor in the ... · carlos henrique tomich de paula da...

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