reversed phase lq

82 Reversed Phase

Reversed Phase 83

* Application data by courtesy YMC Co., Ltd.

Contents

•YMC-PackODS-AQ............................................. 84-87

•YMC-PackODS-A ............................................... 88-89

•YMC-PackODS-AM ............................................ 90-91

•YMC-PackODS-AL ............................................. 92-93

•YMC-PackPolymerC18 ...................................... 94-95

•YMCbasic ........................................................... 96-97

•YMC-PackC8(Octyl) .......................................... 98-99

•YMC-PackPh(Phenyl) .................................... 100-101

•YMC-PackC4(Butyl) ....................................... 102-103

•YMC-PackTMS(C1) ....................................... 104-105

•YMC-PackCN(Cyano) ................................... 106-107

•OrderingInformation ........................................ 108-110

HPLC Columns YMC RP-Classics

HPLC Columns for Reversed Phase Chromatography

InordertosucceedinHPLC,thechoiceoftheoptimalselectivityisessentialtoestablishefficientseparationconditions.Thebestsuitedpackingmaterialdependssignificantlyonthecharacteristicsoftheseparationconditions,whichshouldbethoroughlyconsidered.

ForthispurposeYMCoffersawidevarietyofselectivitiesapplicabletoHPLCfromnano-scaleanalysistolargescaleisolation.Within this catalogue theworld renownYMC-PackODS-Series (YMC-PackODS-AQ,YMC-PackODS-A,YMC-PackODS-AM,YMC-PackODS-AL)andotherphasesaredescribed.

Introduction

84 Reversed Phase

General

YMC-Pack ODS-AQ is a C18 reversed phase silica based HPLC packing material specificallydesignedforusein100%aqueouseluents.Asaresultoftheproprietaryderivatisationprocess,YMC-PackODS-AQexhibitsadifferentselectivitytothatoftraditionalC18stationaryphases.ThisdifferenceinselectivityofYMC-PackODS-AQcanbeusedtoadvantageforHPLCseparations,whicharedifficulttoachievewithconventionalC18columns. Selectivity Data

TheproprietaryYMCderivatisationprocesscreatesthedifferentselectivityofYMC-PackODS-AQ,where:

1.Theactivityofacidicunreactedsilanolsisreduced,allowingmoderatelybasiccompoundstobeelutedwithlittleornopeaktailing.

2.Thebalancedhydrophilic/lipophilicnatureoftheYMC-PackODS-AQstationaryphaseleadstostrongretentionsofpolarsamplesoluteseveninaqueouseluents.

ThesepropertiesofYMC-PackODS-AQarebeneficialforseparationsofpolarorganiccompounds,whichtendnottoberetainedorareunresolvedwhenconventionalC18columnsareused.

ManyconventionalODSpackingslosetheirabilitytoretainpolarcompoundsinthesehighaque-ouscontentmobilephasesasshownopposite.TheyappearlesslipophilicwithdenselyfoldedC18chains.However,insimilarmobilephases,YMC-PackODS-AQmaintainsitsbrush-likeC18chainstructureanditslipophilicpropertiesandprovidesexcellentretentionofpolarcompounds.

Applications

YMC-PackODS-AQisabletoresolvecompoundswithminordifferencesinpolarityfromcloselyrelatedchemicalstructures.Asaresult,YMC-PackODS-AQisanexcellenttoolfortheseparationofdrugsandcorrespondingmetabolites,pesticidesanddegradationproducts,orpeptidesandproteindigestsetc.Thiscapabilityof“polarrecognition”opensupabroadapplicationrangeforYMC-PackODS-AQinlifesciencesandpharmacology.

Genuinelinearscale-upfromanalyticaltolargescaleseparationsiseasilyachievablewithYMCproductssuchasYMC-PackODS-AQ,whereparticlesizesfrom3to150µmareavailableinlargelotsizesuptoseveralhundredkilograms,ifneeded.This,togetherwiththeoutstandingselectivityofYMC-PackODS-AQ,makeitanessentialtooltoenhancetheproductivityoflargescalechro-matographicprocesses.

YMC-PackODS-AQisalsoavailableinpreparativeparticlesizes.

- “hydrophilic” C18- balanced surface chemistry- polar recognition- metabolite recognition

YMC-Pack ODS-AQ

YMC-Pack ODS-AQ Specification

Particle size / µm 3; 5 3; 5Pore size / nm 12 20Surface area / m2g-1 330 175Carbon content / % 14 10Recommended pH range 2.0 - 7.5 2.0 - 7.5

USPL1

Reversed Phase 85


YMC-Pack ODS-AQComparison of ODS-AQ vs. Conventional ODS

ConventionalODS-Column

YMC ODS-AQ

Initial

After24 hoursof flow

After1 hourof flow

Initial

=C18 =Endcapping = Water

=C18 =Endcapping = Water

Conventional ODS instandard organic mobile phases*

Conventional ODS in100% aqueous mobile phases*

YMC-Pack ODS-AQ instandard organic mobile phases*

YMC-Pack ODS-AQ in100% aqueous mobile phases*

Column: YMC-PackODS-A(25µm,12nm)250x10mmIDMobilePhase:100%waterCompounds: Highlypolarandverywatersoluble

Column: YMC-PackODS-AQ(25µm,12nm)250x10mmIDMobilePhase: 100%waterCompounds: Highlypolarandverywatersoluble

86 Reversed Phase

YMC-Pack ODS-AQ

1:Noradrenaline 2:Adrenaline 3:DL-3,4-DihydroxymandelicAcid 4:3,4-Dihydroxyphenylalanine(DOPA) 5:DopamineHydrochloride 6:Tyrosine 7:DL-3-Methoxy-4-HydroxymandelicAcid 8:Phenylalanine 9:4-Hydroxy-3-MethoxyPhenylglycol10:5-Hydroxyindole-3-AceticAcid11:VanillicAcid12:HomovanillicAcid

| | | | 0 10 20 30 min

2

34

56

7

9

12

1

108

11

CatecholaminesColumn: YMC-PackODS-AQ(5µm,12nm)250x4.6mmIDPartNo.: AQ12S05-2546WTEluent: A:phosphatepuffer(100mM,pH3.0)B:acetonitrileGradient: 99%A(0-20min),85%A(20-25min)Flow: 1.5ml/minDetection: UVat210nmTemperature: Roomtemperature

1: Glycolicacid 2: L-Malicacid 3: Malonicacid 4: Lacticacid 5: Aceticacid 6: Maleicacid

7: Citricacid 8: Fumaricacid 9: Succinicacid10: Propionicacid

Exceptional performance for the separation of polar compounds*

No need for ion pair reagents*Strong retention in aqueous eluents*

Cephalosporin antibioticsColumn: YMC-PackODS-AM(5µm,12nm)150x4.6mmIDPartNo.: AM12S05-1546WTEluent: methanol/water/aceticacid(10/85/5)Flow: 1.0ml/minDetection: UVat260nm,0.16AUFSTemperature: 37°C

Cephalosporin antibioticsColumn: YMC-PackODS-AQ(5µm,12nm)150x4.6mmIDPartNo.: AQ12S05-1546WTEluent: methanol/water/aceticacid(10/85/5)Flow: 1.0ml/minDetection: UVat260nm,0.16AUFSTemperature: 37°C

Crude drugsColumn: YMC-PackODS-AQ(5µm,12nm)250x4.6mmIDPartNo.: AQ12S05-2546WTEluent: 20mMH3PO4-NaH2PO4(pH2.8)Flow: 0.7ml/minDetection: UVat220nm,0.08AUFSTemperature: 30°CInjection: 10µl(0.007~1.8mg/ml)

Conventional ODS

YMC-Pack ODS-AQ

51015min 5101520min

Reversed Phase 87


YMC-Pack ODS-AQ

1: Cytosine 2: Cytidine 3: Uracil 4: Deoxycytidine 5: Guanine 6: Adenine 7: Hypoxanthine 8: Uridine 9: Xanthine10: Thymine11: Inosine12: Guanosine13: Deoxyinosine14: Deoxyguanosine15: Xanthosine16: Thymidine17: Adenosine18: Deoxyadenosine

BycourtesyofDr.ManfredRaida,IPFHannover

1:Arbutin 2: Salicin 3: Esculine 4: g-Strophanthin (Quabain) 5: Baicalin 6: Barbaloin 7: Glycyrrhizin 8: Digitoxin

Comparison of YMC-Pack ODS-AQ with competitive products

Separation of biomolecules* Excellent choice for a broad chromatographic appli-cation range*

YMC-PackODS-AQ

Since1987,YMC-PackODS-AQhasconsistentlyincreaseditspopularityduetoitsnovelselectivitypatterntowardspolarcompoundsanditsabilitytowithstand100%aqueousconditions.Today,morethan25(!)yearslater,manynewanalyticalandpreparativemethodsarestillbeingdevelopedonYMC-PackODS-AQchemistrydespiteofvariousAQ-typeproductsrecently beingintroducedbyourcompetitors;phaseswithsupposedly“identical”selectivityorwithexoticbondingtechniquesdesignedtogenerateperformancecharacteristicssimilartothoseofYMC-PackODS-AQ.However,genuineYMC-PackODS-AQstillrepresentstodayafullycompetitivestate-of-the-arthighperformancestationaryphase,despitethecomplementaryYMCinnovation,namelynewgenerationHydrosphereC18,describedonpages78-79,asapotentialin-housecompetitor.

ReproSil-Pur C18-AQ

Tryptic digest of BSA

Formoreapplicationspleaserefertoour“ApplicationDataCollections”orcontactusdirectly.

Column care

TherecommendedpHrangeforYMC-PackODS-AQis2.0-7.5inupto100%aqueoussystemsandamaximumof50°C.Removeacidandbuffersaltsbeforestorage.Storethecolumninmethanol/water=70/30.Cloggedinletfritsoftencanbecleanedbychangingtheflowdirection.

Fordetailedinformationpleaserefertothe“ColumnCareandUseInstructions”,whichareshippedwitheachanalyticalcolumn.

| | | | 0 10 20 30 min

| | | | | 0 10 20 30 40 min

NucleosidesColumn: YMC-PackODS-AQ(5µm,12nm)250x4.6mmIDPartNo.: AQ12S05-2546WTEluent: A=20mMCH3COOH-CH3COONH4(pH3.5) B=20mMCH3COOH-CH3COONH4(pH3.5)/methanol=90/10(v/v)Gradient: 30%B(0-5min),30-100%B(5-13min,linear),100%B(13-40min)Flow: 0.7ml/minDetection: UVat260nmTemperature: 30ºC

Crude drugsColumn: YMC-PackODS-AQ(5µm,12nm)250x4.6mmIDPartNo.: AQ12S05-2546WTEluent: A:methanol/NH4H2PO4(20mM)=5/95 B:methanol/NH4H2PO4(20mM)=80/20Gradient: 0-100%B(0-20min,linear),100%B(20-40min)Flow: 0.6ml/minDetection: UVat250nmTemperature: 30°C

niceresolution

88 Reversed Phase

General

YMC-PackODS-A,YMC’sclassicalreversedphasepackingmaterial,isrenownedworldwidebe-causeofitsuniqueperformanceandreproducibility.Duetothehighquality,YMC-PackODS-AiswidelyusedforthevalidationofanalyticalHPLCmethodsandforlong-termreproducibleprepara-tiveHPLCprocesses.

Properties

The production of the base silica forYMC-Pack ODS-A and its subsequentderivatisationareperformedinlargebulkbatches.Exhaustiveendcappingreduc-esreliablytheactivityofsilanolgroupsand minimises nonspecific secondaryretention.

Inadditiontostandardmethods,likede-terminationofadsorptionisotherms,par-ticlesizedistributionandcarboncontent(seetableabove),YMCusesanextensiverange of analytical methods to ensureconstantandreproducibleselectivityofthereversedphasepackings.

ThebaseofYMC-PackODS-AisYMC’shigh purity silica. This premium silicacontains only very low levels of metalcontaminants and so prevents signifi-cant tailing of sample molecules suchas8-hydroxyquinolineoracetylacetone,which easily form coordination com-plexeswithmetalionsonthesilicasur-face.Ascoordinatingfunctionalgroupsare frequent structural components inpharmaceuticalcompounds,highpuritypackings such as YMC-Pack ODS-Aareneededforreproducibleseparationofthesecompoundswithoutsecondaryretentionortailing.

YMC-Pack ODS-A is also available inpreparativeparticlesizes.

- fully endcapped C18 material

- highly versatile ODS phase

- for polar to moderately nonpolar pharmaceuticals, organic chemicals, biologicals and natural products

YMC-Pack ODS-A* Silica Basewith MetalImpurities*

a bColumn: YMC-PackODS-A(12nm,5µm,150x4.6mmID)Eluent: KH2PO4(20mM,pH7.6)/methanol=40/60Flow: 1.0ml/minDetection: UV,254nmTemperature: 37°CSubstances: 1.Uracil 2.Acetylacetone 3.8-Hydroxyquinoline

Coordination compoundsa: High purity packing material YMC-Pack ODS-Ab: Competitive product

12

3

1

2

3

YMC-Pack ODS-A

YMC-Pack ODS-A Specification

Particle Size / µm 3; 5 3; 5 5Pore Size / nm 12 20 30Surface area / m2g-1 330 175 100Carbon content / % 17 12 7Recommended pH range 2.0 - 7.5 2.0 - 7.5 2.0 - 7.5

USPL1

Reversed Phase 89


YMC-Pack ODS-A

| | | | | 0 5 10 15 20 min

1

2

3

4

5

6

7

89

10

11

12

1: Olaquindox 2: Carbadox 3: Thiamphenicol 4: Sulfamerazine 5: Sulfadimidine 6: Sulfamonomethoxine 7: Furazolidone 8: OxolinicAcid 9: Sulfaquinoxaline10: Sulfadimethoxine11: NalidixicAcid12: PiromidicAcid

| | | | 0 10 20 30min

1: Hydrocortisone 2: Cortisone 3: Methylprednisolone 4: Betamethasone 5: Dexamethasone 6: Corticosterone 7: Beclomethasone 8: Triamcinolone Acetonide

Column: YMC-PackODS-A(5µm,12nm)250x4.6mmIDPartNo.: AA12S05-2546WTEluent: A:ACN/NH4H2PO4(50mM)=10/90 B:ACN/NH4H2PO4(50mM)=80/20Gradient: 25%B(0-5min),25-100%B(5-15min), 100%B(15-20min)Flow: 1.0ml/minDetection: UV,240nmTemperature: 37ºC

Adrenocorticosteroids*Antibacterial agents*

Column: YMC-PackODS-A(5µm,12nm)150x4.6mmIDPartNo.: AA12S05-1546WTEluent: ACN/H2O=27/73Flow: 1.0ml/minDetection: UV,260nmTemperature: 37ºC

Column Care

TherecommendedpHrangeforusingYMC-PackODS-Acolumnsis2.0-7.5.Removeacidandbuffersaltsbeforestor-age.Storethecolumninmethanol/water=50/50.Ifcolumnsareaffectedbyundesiredcontaminantsorcloggedinletfritswhichcausebackpressureincreases,flushthecolumnwithTHFintheoppositeflowdirection.

Fordetailedinformationpleaserefertothe“ColumnCareandUseInstructions”whichareshippedwitheachanalyticalcolumn.

Applications

YMC-PackODS-Ais frequentlyusedforpharmaceutical,biochemicalandenvironmentalapplicationsaswellasforseparationsinthefieldoffoodtechnology.

YMC-PackODS-Aisavailableinparticlesizesfrom3to50µm.Astheselectivityisidenticalthroughoutthewholerange,thesephasesareidealforscale-upfromanalyticaltopreparativeprocessscale.

Column: YMC-PackODS-A(5µm,12nm)250x4.6mmIDPartNo.: AA12S05-2546WTEluent: 20mMtriethylamine-aceticacid(pH5.7)/acetonitrile(9/1)Flowrate: 1.0ml/minDetection: UVat260nm,0.16AUFSTemperature: 37ºCInjection: 5µl(0.27~0.71mg/ml)

Column: YMC-PackODS-A(5µm,12nm)150x4.6mmIDPartNo.: AA12S05-1546WTEluent: A:water B:methanol 2%B(0-5min),2-30%B(5-15min,linear), 30-100%B(15-20min,linear),100%B(20-30min)Flowrate: 1.0ml/minDetection: EvaporativeMassAnalyzer(ACSLtd.) Temp 150 TimeConstant 5 PMT 3 Attenuation 16 Pressure 20psiTemperature: 30ºCInjection: 200µl(1.5~15mg/ml)

Sugar nucleotides* Cyclodextrins*

102030min

51015202530min

1. g-Cyclodextrin 2. a-Cyclodextrin 3. b-Cyclodextrin 4. O-Dimethyl-b-Cyclodextrin 5. O-Trimethyl-b-Cyclodextrin

1. CDP-D-glucose 2. UDP-D-glucose 3. UDP-N-acetyl-D-glucosamine 4. GDP-D-mannose 5. ADP-D-glucose

90 Reversed Phase

General

ValidationandregistrationofanalyticalHPLCmethodsrequiresthelongtermreproducibilityoftheentireanalyticalprocess.ThehighconsistencyinthequalityofHPLCpackingsandcolumnsplaysakeyroleforvalidatedHPLCanalysis.Therefore,YMCcreatedODS-AM,ahighqualityreversedphaseC18HPLCpackingmaterial tomeet themoststringentdemands forvalidatedanalyticalHPLCprocesses.

Properties

YMC-PackODS-AMisproducedinlargelotsusinghighpurityYMCsilicaasabasematerialandamultistagesynthesisprocess.Forthederivatisation,monomericbondingchemistryisappliedfollowedbyanextensiveendcappingprocesstoreducethesilanolactivity.

The resulting YMC-Pack ODS-AM packing isextensively tested to ensure compliance withspecificationssetforverylowvariationsinphysi-cochemicalproperties.

Inaddition,YMC-PackODS-AMpackingsandcolumns have to pass numerous proprietarychromatographicteststomeetthenarrowqualityspecificationrangewithregardto:

-selectivitypattern-columnresolution-absoluteretention-peaksymmetry YMCappliesvariousteststoperformstatisticalqualitycontrolforreversedphaseHPLCpack-ings.Thea-valuetestofmethylparabenand2,6-dimethylpyridineforinstance,isverysensitiveandisroutinelyusedtomonitortheretentionandtheselectivitypropertiesofYMC-PackODS-AM.

Methylparabenisamoderatelypolar, inertcompound.It isretainedsolelybyaRPmechanism,withminimalsecondary interactionswithresidualsilanolgroups.2,6-dimethylpyridine,however,representsalipophilicaminecompoundwhichhasahighpotentialofunspecificinteractionwithunreactedacidicsilanols.Anincreaseinretentionof2,6-dimethylpyridineandhencelowera-valueswouldindicateincompleteC18bondingand/orineffectiveendcapping.YMCspecifiesforODS-AMthatthestatisticala-valueofmethylparabenand2,6-dimethylpyridinebe2.77+/-0.20.

TherigorousqualitycontrolandthequalityassurancesystemappliedbyYMCminimisesthevari-ationinretentionandselectivityofYMC-PackODS-AMcolumns.

Duetotheguaranteedlongtermreproducibility,YMC-PackODS-AMcolumnsoftenarethefinalchoiceforestablishingvalidatedHPLCanalysis.

- high quality analytical C18- tightly specified- long term reproducibility- for method validation- for method registration

a: Methylparaben/2,6-Dimethylpyridine as reference

Statistical Quality Control (a-value monitoring)

a

Consecutive Lots

YMC-Pack ODS-AM

YMC-Pack ODS-AM Specification

Particle Size / µm 3; 5Pore Size / nm 12Surface area / m2g-1 330Carbon content / % 17Recommended pH range 2.0 - 7.5

USPL1

Reversed Phase 91


YMC-Pack ODS-AMApplications

ODS-AMhasanappropriateselectivityforpolartomoderatelynonpolarpharmaceuticals,organicintermediates,biologi-calandnaturalproductsfoundinthechemicalandpharmaceuticalindustry.

Nucleotides*

Column: YMC-PackODS-AM(5µm,12nm)250x4.6mmIDPartNo.: AM12S05-2546WTEluent: A=0.2Mtriethylamine-aceticacid(pH6.6) B=0.2Mtriethylamine-aceticacid(pH6.6)/ACN(95/5)Gradient: 4%B(0-10min),4-100%B(10-35min,linear), 100%B(35-50min)Flow: 1.0ml/minDetection: UVat260nm,0.16AUFSTemperature: 30°CInjection: 14µl(0.029~0.16mg/ml)

Column: YMC-PackODS-AM(5µm,12nm)250x4.6mmIDPartNo.: AM12S05-2546WTEluent: A=60mMCH3COONa-CH3COOH(pH6.6)/ACN(94/6) B=60mMCH3COONa-CH3COOH(pH6.6)/ACN(40/60)Gradient: 0-55%B(0-20min,linear),100%B(20-25min),0%B(25-35min)Flow: 1.0ml/minDetection: UVat250nm,0.08AUFSTemperature: 37ºCInjection: 5µl(62.5pmol/µl)Sample: PTCderivatives

PTC-amino acids*

1: L-Asparticacid 2: L-Glutamicacid 3: L-Serine 4: Glycine 5: L-Histidine 6: L-Arginine 7: L-Threonine 8: L-Alanine 9: L-Proline

10: NH311: L-Tyrosine12: L-Valine13: L-Methionine14: L-Cystine15: L-Isoleucine16: L-Leucine17: L-Phenylalanine18: L-Lysine

Column Care

TherecommendedpHrangeforusingYMC-PackODS-AMcolumnsis2.0-7.5.Removeacidandbuffersaltsbeforestorage.Storethecolumninmethanol/water=50/50.Ifcolumnsareaffectedbyundesiredcontaminantsorcloggedinletfritswhichcausebackpressureincreases,flushthecolumnwithTHFintheoppositeflowdirection.


10203040min

1: 5‘-CMP 2: 5‘-UMP 3: 5‘-dCMP 4: 5‘-CDP 5: 5‘-GMP 6: 5‘-UDP 7: 5‘-IMP 8: 5‘-CTP 9: 5‘-UTP10: 5‘-GDP11: 5‘-TMP12: 5‘-AMP13: 5‘-dGMP14: 5‘-GTP15: 5‘-TDP16: 5‘-ADP17: 5‘-TTP18: 5‘-dAMP19: 5‘-ATP

51015202530min

92 Reversed Phase

YMC-Pack ODS-AL

General

YMC-PackODS-ALusesnotonlyhydrophobic interactionbutalsosecondary interactionswithreactiveresidualsilanolgroupstoaffectseparation.Thisresultsinadifferentselectivityfromcon-ventionalODScolumns.Whenionicinteractionsareinvolved,itispreferabletouseabufferinthemobilephasetoachievereproducibleseparations.

- residual silanols for mixed-mode separations

- same high Iigand density as other YMC ODS phases

- unique selectivity for polar compounds

YMC-Pack ODS-AL Specification

Particle Size / µm 3; 5Pore Size / nm 12Surface area / m2g-1 330Carbon content / % 17Recommended pH range 2.0 - 7.5

Effects of silanol groups*

YMC-Pack ODS-AL YMC-Pack ODS-AM

1:Uracil2:Pyridine3:2,6-diemetylpyridine4:Methylparaben

Theseparationfactor(a)ofinternalstandardsmethylparaben/2,6-dimethylpyridineforYMC-PackODS-AL,whichisnotendcappedisdifferenttothatofYMC-PackODS-AM.Duetotheresidualsilanolgroups,YMC-PackODS-ALshowsrelativelygreatretentionofpyridines.

USPL1

Reversed Phase 93


YMC-Pack ODS-AL

Steroids from toad venom*

Column care

TherecommendedpHrangeforusingYMC-PackODS-ALcolumnsis2.0-7.5.Removeacidandbuffersaltsbeforestor-age.Storethecolumninmethanol/water=50/50.Ifcolumnsareaffectedbyundesiredcontaminantsorcloggedinletfritswhichcausebackpressureincreases,flushthecolumnwithTHFintheoppositeflowdirection.


Column: YMC-PackODS-AL(5µm,12nm)150x4.6mmIDPartNo.: AL12S05-1546WTEluent: 100mMKH2PO4-Na2HPO4(pH7.0)/methanol(75/25)Flow: 0.8ml/minDetection: UVat270nm,0.13AUFSTemperature: 30ºCInjection: 3µl(0.1~0.8mg/ml)

Column: YMC-PackODS-AL(5µm,12nm)150x4.6mmIDPartNo.: AL12S05-1546WTEluent: ACN/0.1%phosphoricacid(45/55)Flow: 1.0ml/minDetection: UVat300nm,0.08AUFSTemperature: 40°CInjection: 10µl(0.04~0.08mg/ml)

Applications

Vitamin D2 and Vitamin D3*Sterols*

Column: YMC-PackODS-AL(5µm,12nm)150x4.6mmIDPartNo.: AL12S05-1546WTEluent: acetonitrile/water(95/5)Flow: 1.0ml/minDetection: UVat265nmTemperature: 40ºCInjection: 10µl(0.01mg/ml)

Column: YMC-PackODS-AL(5µm,12nm)250x4.6mmIDPartNo.: AL12S05-2546WTEluent: methanol/water(100/2)Flow: 0.8ml/minDetection: UVat210nm,0.32AUFSTemperature: 37°CInjection: 10µl(0.4~1mg/ml)

51015min

10203040min

Disinfectants*

94 Reversed Phase

- hydrophilic polymethacrylate support

- excellent reproducibility of C18 chemistry integral to polymer matrix

- no silanol or metal contaminants

- pH stable from pH 2 - 13

- compatible with all standard reversed phase solvents

YMC-Pack PolymerC18

YMC-Pack PolymerC18 Specification

Particle Size / µm 6Pore Size / nm proprietarySurface area / m2g-1 n/aCarbon content / % 10Recommended pH range 2.0 - 13.0

2-13

General

YMC-PackPolymerC18isareversedphaseliquidchromatographypackingwhichprovidesabroadrangeofsolventchoicesandapHrangefrom2.0-13.YMC-PackPolymerC18ismanufacturedfromahydrophilicmethacrylatepolymerwhichiscross-linkedwithC18ligand-containingreagents.YMC-PackPolymerC18offersamaximumapplicationrange:awidevarietyofcompoundssuchasorganicacids,organicamines,peptides,pharmaceuticalsandproteinscanbeseparatedusingYMC-PackPolymerC18.

Properties

YMC-PackPolymerC18ispreparedfromahydrophilicmethacrylatepolymerbondedwithahydro-phobicoctadecylsilanereagenttomaketheC18functionalityanintegralpartofthepolymericstruc-ture.Thisgivesathree-dimensionalpolymermatrixwhichisnotbasedonasilicagelsupport.

Assuch, ithasno residualsilanolsormetal impurities to interferewith theseparationofbasicorganiccompounds.

YMC-Pack PolymerC18 is compatible with all common reversed phase eluents such as water,methanol,acetonitrileandTHF.Virtuallyallaqueousbuffersandacidmodifiers,suchasTFAandphosphoricacid,aswellasbasemodifierssuchassodiumhydroxideandammoniumhydroxidecanbeused.Sinceitresistsshrinkingandswelling,YMC-PackPolymerC18canbeusedwitheluentsrangingincompositionfrom100%aqueousto100%organiccomponent.

Inaddition,YMC-PackPolymerC18caneasilybesterilisedbyflushingwith0.1MNaOHin20%acetonitrile/water.

TheselectivityandretentionofYMC-PackPolymerC18issimilartostandardODSphases,duetoitshydrophobicbondingonahydrophilicsupport.Consequently,itsselectivityisclosertothatofsilica-basedC18supportsthantostyrene/DVB-basedsupports.

Itshouldbenotedthatinteractionsbetweenaromaticorconjugatedsystemsandthemethacrylatebackboneprovidesslightlygreaterretentionwhencomparedtosilica-basedODScolumns,whereashighlyaliphaticcompoundsshowgreaterretentiononsilica-basedODSsupports.

YMC-PackPolymerC18isalsoavailableinpreparativeparticlesizes.

Reversed Phase 95


YMC-Pack PolymerC18

Aminoalcohols*

Column: YMC-PackPolymerC18250x6.0mmIDPartNo.: PC99S06-2506WTEluent: 100mMNa2HPO4/100mMNaOH(60/40,pH12.0)Flow: 0.6ml/minDetection: UVat215nm,0.32AUFSTemperature: 20°CInjection: 50µl(0.2~3.0mg/ml)

Peptides and proteins*

Column: YMC-PackPolymerC18150x4.6mmIDPartNo.: PC99S06-1546WTEluent: A=acetonitrile/water/TFA(20/80/0.05) B=acetonitrile/water/TFA(45/55/0.05) 0-100%B(0-30min,linear)Flow: 1.0ml/minDetection: UVat220nm,0.32AUFSTemperature: 30ºCInjection: 30µl

1: Met-Enkephalin 2: Leu-Enkephalin 3: BSA 4: a-Matingfactor 5: Insulin 6: Ovalbumin

Column care

YMC-PackPolymerC18isstabletowardshydrolysisbetweenpH2.0-13.0.Removeacidandbuffersaltsbeforestorage.Storethecolumninmethanol/water=70/30.Ifcolumnsareaffectedbyundesiredcontaminantsorcloggedinletfritswhichcausebackpressureincreases,flushthecolumnwithTHFintheoppositeflowdirection.


Applications

Organic acids*

Column: YMC-PackPolymerC18250x4.6mmIDPartNo.: PC99S06-2546WTEluent: 0.1%TFAFlow: 0.5ml/minDetection: UVat220nm,0.08AUFSTemperature: 30°CInjection: 10µl(0.016~2.2mg/ml)

1020min

1: Ethanolamine 2: Diethanolamine 3: N-Methylethanolamine 4: Triethanolamine 5: N,N-Dimetylethanolamine

5101520253035min

1. L-Mailicacid 2. Lacticacid 3. Aceticacid 4. Maleic acid 5. Succinicacid 6. Propioniacid 7. Fumaricacid

96 Reversed Phase

YMCbasic- alternative bonding approach to reduce peak tailing of basic pharmaceuticals

- no need for ion pair reagents or amine modifiers

- complementing selectivity to C8 and C18 materials

YMCbasic Specification

Particle size / µm 3; 5Pore size / nm proprietarySurface area / m2g-1 proprietaryCarbon content / % 7.5Recommended pH range 2.0 - 7.5

General

The proprietary derivatisation pro-cedureforYMCbasicallowsYMCtoproduce a material with controlledsurface coverage, which shows ex-cellent lot-to-lot reproducibilityasaresultofcloselymonitoringboththeproductionofthesilicasupportandthebondingprocess.

The resulting YMCbasic materialshows a different hydrophobicity toC8 or C18 phases as shown in thediagramonthispage.Finally, it rep-resents an interesting alternative toshortchainselectivities.

Applications

The result is a phase with true re-versed phase characteristics, highresolution and excellent peak sym-metry forbasiccompoundswithouttheneedforionpairreagentsoraminemodifiers(seeseparationofanilinesusingacetonitrile/watereluent).Un-likemanybase-deactivatedphases,YMCbasicisalsosuitableforseparationofacidiccompounds,showingslightretentionofhighlypolaracidcompoundssuchasmaleate.YMCbasicprovidesacomplementingselectivityseenwithconventionalC8andC18materials,butwithoutpeaktailingforbasiccompounds.

YMCbasicisalsoavailableinpreparativeparticlesizes.

YMCbasicYMC-Pack OctylYMC-Pack ODS-AM

Hydrophobicity of YMCbasic versusYMC-Pack Octyl and YMC-Pack ODS-AM*

Column: 250x4.6mmIDEluent: acetonitrile/water(75/25)Flow: 1.0ml/minDetection: UVat254nm

5.12

5 To

luen

e

5.79

2 E

thyl

ben

zene

6.80

8 P

rop

ylb

enze

ne

8.16

7 B

utyl

ben

zene

7.20

0

12.1

25

USPL7

Reversed Phase 97


YMCbasic

1. Phenol 2. Dimethylaniline 3. Toluene 4. Diethylaniline

Anilines and aromatic compounds* Basic compounds*

1. Maleate 2. Phenylpropanolamine 3. Pseudoephedrine 4. Scopalamine 5. Chlorpheniramine

Cough/cold medicine*

1. Uracil 2. Procainamide 3. N-Acetylprocainamide 4. 4-Hydroxyacetophenone

Formoreapplicationspleaserefertoour“ApplicationDataCollections”orcontactusdirectly.

Column care

TherecommendpHrangeforYMCbasicis2.0-7.5.Removeacidandbuffersaltsbeforestorage.Storethecolumninmethanol/water=70/30.Cloggedinletfritsoftencanbecleanedbychangingtheflowdirectionorreplacement.


Column: YMCbasic(5µm)250x4.6mmIDPartNo.: BA99S05-2546WTEluent: acetonitrile/water(60/40)Flow: 2.0ml/minDetection: UVat254nm

Column: YMCbasic(5µm)250x4.6mmIDPartNo.: BA99S05-2546WTEluent: acetonitrile/0.05mMKH2PO4(pH3.2)(15/85)Flow: 2.0ml/minDetection: UVat254nm

Column: YMCbasic(5µm)150x4.6mmIDPartNo.: BA99S05-1546WTEluent: 20mMK2HPO4-KH2PO4(pH6.9)/acetonitrile(92/8)Flow: 1.0ml/minDetection: UVat254nm,0.32AUFSTemperature: 37°CInjection: 10µl(30~300µg/ml)

1.534.56min

2468min

102030min

98 Reversed Phase

- alternative phase to C18 with moderate hydrophobicity

- fully endcapped, high coverage monomeric bonded chemistry

- ideal for method development and routine separations

- excellent retention for all types of organic molecules, especially peptides, proteins and pharmaceuticals

YMC-Pack C8 (Octyl)

YMC-Pack C8 Specification


General

YMC-PackC8isoneofYMC’smostcommonlyusedbondedphasesandanexcellentalternativetoC18selectivities.Duetoitsmoderatehydrophobicity,retentiontimestendtobeshorterthanthoseforODSphases.YMC-PackC8issuitableforawiderangeofsampletypesincludingpharmaceu-ticalsandbiologicalswitharelativelyhighhydrophobicity.

Properties

YMC-PackC8ispreparedbyexhaustivebondingofamonomericoctylsilanetototallysphericalandporoussilicagel.Thebondedphaseisthentreatedwithanexhaustiveendcappingprocesstoensureahighsurfacecoverageleadingtoamoderate10%carbonloadingonthestandard12nmporematerial.ComparedtoC18phases,retentiontimesforhydrophobicmoleculeswillbeshorteronC8materialduetothereducedcarbonload.

YMC-PackC8isideallysuitedfortheseparationofmanycompoundsthataretoostronglyretainedonC18phasesorwhichrequiregreaterretentionthanprovidedbyC4materials.Itisoneofthemostversatile reversedphasematerialsandshouldbeconsidered for thedevelopmentofnewmethods.

Available in three porosities,YMC-Pack C8materialwill separatemanyclasses of compoundsincludingpharmaceuticals,organicchemicals,peptides,proteinandotherbiologicalmolecules.Forpreparativeapplications,choosethesmallestporesizewhichprovidesadequateretentionandresolution.Thisisbecausesampleloadingisgenerallyproportionaltosurfacearea.Smallerporositymediaprovidegreatersurfaceareaandhencegreaterloadability.

YMC-PackC8(Octyl)isalsoavailableinpreparativeparticlesizes.

Column care

YMC-PackC8(Octyl)isstabletowardshydrolysisbetweenpH2.0-7.5.Removeacidandbuffersaltsbeforestorage.Storethecolumninmethanol/water=70/30.Ifcolumnsareaffectedbyundesiredcontaminantsorcloggedinletfritswhichcausebackpressure increases,flushthecolumnwithTHFintheoppositeflowdirection.Fordetailedinformationpleaserefertothe“ColumnCareandUseInstructions”whichareshippedwitheachanalyticalcolumn.

USPL7

Reversed Phase 99


YMC-Pack C8 (Octyl)

Column: YMC-PackC8(Octyl)(5µm,12nm)250x4.6mmIDPartNo.: OC12S05-2546WTEluent: acetonitrile/THF/water(46/4/50)Flow: 0.7ml/minDetection: UVat230nm,0.16AUFSTemperature: 30ºCInjection: 7µl(0.1mg/ml)

Nicotinic acid analogues*Estrogens and progestins*

Column: YMC-PackC8(Octyl)(5µm,12nm)150x4.6mmIDPartNo.: OC12S05-1546WTEluent: acetonitrile/20mMKH2PO4(5/95)Flow: 1.0ml/minDetection: UVat260nm,0.64AUFSTemperature: 30ºCInjection: 13µl(0.2mg/ml)

Applications

Column: YMC-PackC8(Octyl)(5µm,12nm)150x4.6mmIDPartNo.: OC12S05-1546WTEluent: methanol/100mMKH2PO4(10/90)Flow: 0.8ml/minDetection: UVat285nm,0.16AUFSTemperature: 37ºCInjection: 10µl(0.05~0.4mg/ml)

Resorcylic acid isomers*

204060min

510min

102030min

100 Reversed Phase

- fully endcapped, monomeric phenyl phase

- unique selectivity due to π - π interactions

- preferential retention of aromatic compounds

- alternative selectivity to C18, C8 or C4 bonded phases for the analysis of peptides and other biomolecules

YMC-Pack Ph (Phenyl)

YMC-Pack Ph Specification

Particle Size / µm 3; 5 5Pore Size / nm 12 30Surface area / m2g-1 330 100Carbon content / % 9 3Recommended pH range 2.0 - 7.5 2.0 - 7.5

USPL11

General

YMC-PackPh(Phenyl)isahighdensitybondedphase(9%carbonloadon12nmsilica)whichisfullyendcapped.Thisresultsinasuperiorbondedphasewithprovenperformanceandexceptionallifetimeforaphenylreversedphasecolumn.

Properties

YMC-PackPh (Phenyl)providesauniqueselectivitywhencompared toaliphaticstraightchainreversedphasessuchasC18,C8orC4.Theπ-electronsofthephenylgroupscaninteractwitharomaticresiduesofananalytemoleculeinadditiontohydrophobicinteractionstoincreasereten-tionrelativetonon-aromaticspecies.

PhenylphasesareconvenientfortheseparationofaromaticcompoundsandalsoprovideausefulalternativetoC18orC4phasesfortheseparationofpeptidesandproteinsonbothsmallpore(12nm)andwidepore(30nm)materials.Retentionisdecreasedonwideporephenylphasesrelativeto12nmphenylmaterialduetothelowersurfaceareaofthewideporematerial.

YMC-PackPh(Phenyl)isalsoavailableinpreparativeparticlesizes.

Reversed Phase 101



Column: YMC-PackPh(5µm,12nm)250x4.6mmIDPartNo.: PH12S05-2546WTEluent: 100mMtriethylamine-aceticacid(pH6.0)Flow: 1.0ml/minDetection: UVat260nm,0.16AUFSTemperature: 37ºCInjection: 5µl(0.27~0.71mg/ml)

Sugar nucleotides*

1: CDP-D-glucose 2: UDP-D-glucose 3: UDP-N-acetyl-D-glucosamine 4: GDP-D-mannose 5: ADP-D-glucose

Water-soluble vitamins*

Column: YMC-PackPh(5µm,12nm)150x4.6mmIDPartNo.: PH12S05-1546WTEluent: acetonitrile/50mMNH4H2PO4(10/90)Flow: 1.0ml/minDetection: UVat210nm,0.16AUFSTemperature: 37ºCInjection: 10µl(0.02~0.30mg/ml)

1: p-Aminobenzoicacid(PABA) 2: Biotin(VitaminH) 3: Riboflavin(VitaminB2) 4: Cyanocobalamin(VitaminB12)

Column care

YMC-PackPh(Phenyl)isstabletowardshydrolysisbetweenpH2.0-7.5.Removeacidandbuffersaltsbeforestorage.Storethecolumninmethanol/water=70/30.Ifcolumnsareaffectedbyundesiredcontaminantsorcloggedinletfritswhichcausebackpressureincreases,flushthecolumnwithTHFintheoppositeflowdirection.


Column: YMC-PackPh(5µm,12nm)250x4.6mmIDPartNo.: PH12S05-2546WTEluent: 5mMaceticacidFlow: 1.0ml/minDetection: UVat280nm,0.32AUFSTemperature: 25ºCInjection: 20µl

Polyphenols* Fullerenes*

Column: YMC-PackPh(5µm,12nm)150x6.0mmIDPartNo.: PH12S05-1506WTEluent: hexane/2-propanol(50/50)Flow: 1.0ml/minDetection: UVat350nm,0.08AUFSTemperature: ambient(25ºC)Injection: 4µl(0.125mg/ml)

1

2

3

4

1: Pyrogallol 2: Hydroquinone 3: Catechol 4: Phenol

102030min 1020min

51015min

102 Reversed Phase

General

DuetoshorteralkylchainsYMC-PackC4hasalowerhydrophobicitythanbothC18andC8phases.Thereforeretentiontimesofnon-polarsamplestendtobeshorteronYMC-PackC4,makingitanidealchoiceforfasterseparations.

Properties

YMC-PackC4phasesarelesshydrophobicandgenerallyrequiremoreaqueousbufferthanC8orC18phases.WhencomparedtoC8orC18packingsusingthesameeluent,YMC-PackC4showssignificantlyshorterretentiontimesfornonpolarcompounds.Retentionofpolarcompounds,how-ever,isnotsignificantlyaffected.Therefore,mixtureswithawiderangeofcomponentpolarityarebestseparatedbyYMC-PackC4.Thisisbecausethebutylbondedphasegivesshorterretentiontimeswhilestillmaintaininghighresolutionwhencomparedtolongerchainbondedchemistries.

YMC-Pack C4 Specification


- low hydrophobicity material

- high coverage monomeric bonded chemistry

- ideally suited for separation of biological materials

YMC-Pack C4 (Butyl)

Column: YMC-PackC4(5µm,30nm)150x4.6mmIDEluent: A=water/TFA(100/0.1) B=acetonitrile/2-propanol/TFA(50/50/0.1);30-75%B(0-15min),75%B(15-20min)Flow: 1.0ml/minDetection: UVat220nmTemperature: 37°CInjection: 10µl(0.25~1.0mg/ml)

Proteins (MW 66,000 – 96,000)*

1: BSA (MW66,000) 2: Conalbumin(Chickeneggwhite) (MW77,000) 3: Lipoxidase(Soybean) (MW96,000)

*Impuritiesincommerciallipoxidase

USPL26

Reversed Phase 103


YMC-Pack C4 (Butyl)

Anti-human immunodeficiency virus (HIV) agents*

Column care

YMC-PackC4isstabletowardshydrolysisbetweenpH2.0-7.5.Removeacidandbuffersaltsbeforestorage.Storethecolumninmethanol/water=70/30.Ifcolumnsareaffectedbyundesiredcontaminantsorcloggedinletfritswhichcausebackpressureincreases,flushthecolumnwithTHFintheoppositeflowdirection.


2,4-Dinitrophenylhydrazones of aldehydes andketones*

Column: YMC-PackC4(5µm,12nm)150x4.6mmIDPartNo.: BU12S05-1546WTEluent: A=tetrahydrofuran/water(10/90) B=acetonitrile;35%B(0-7min),35-65%B(7-18min,linear), 100%B(18-19min),35%B(19-35min)Flow: 1.5ml/minDetection: UVat360nm,0.01AUFSTemperature: 30°CInjection: 11µl(0.0025mg/ml)

Column: YMC-PackC4(5µm,12nm)150x4.6mmIDPartNo.: BU12S05-1546WTEluent: methanol/10mMKH2PO4(10/60)Flow: 1.0ml/minDetection: UVat254nm,0.16AUFSTemperature: 37ºCInjection: 7µl(0.125mg/ml)

1: Formaldehyde2,4-DNPH 2: Acetaldehyde2,4-DNPH 3: Acetone2,4-DNPH 4: Acrolein2,4-DNPH 5: Propionaldehyde2,4-DNPH 6: Crotonaldehyde2,4-DNPH 7: Methylethylketone2,4-DNPH 8: Isobutyraldehyde2,4-DNPH 9: Benzaldehyde2,4-DNPH10: n-Valeraldehyde2,4-DNPH11: p-Tolualdehyde2,4-DNPH12: Capronaldehyde2,4-DNPH

Column: YMC-PackC4(5µm,30nm)150x4.6mmIDPartNo.: BU30S05-1546WTEluent: A)acetonitrile/water/TFA(5/95/0.1) B)acetonitrile/water/TFA(60/40/0.1)Flow: 1.0ml/minDetection: UVat220nm,0.32AUFSTemperature: 37ºCInjection: 16µl(0.16~0.33mg/ml)

Proteins* Water-soluble vitamins*

Column: YMC-PackC4(5µm,12nm)150x4.6mmIDPartNo.: BU12S05-1546WTEluent: acetonitrile/50mMNH4H2PO4(10/90)Flow: 1.0ml/minDetection: UVat210nm,0.16AUFSTemperature: 37ºCInjection: 10µl(0.02~0.30mg/ml)

1: RibonucleaseA 2: Cytochromec 3: Lysozyme 4: Myoglobin

1: p-Aminobenzoicacid(PABA) 2: Biotin(VitaminH) 3: Riboflavin(VitaminB2) 4: Cyanocobalamin(VitaminB12)

510152025min

1020min

1020min1020min

104 Reversed Phase

General

YMC-PackC1(TMS)isabondedphasesuitableforsamplesthatexhibitstrongretentionchar-acteristicsandaredifficultorimpossibletoseparateonconventionalreversedphaseornormalphasecolumns.

Properties

YMC-PackTMS(C1)isbondedwithtrimethylmonochlorosilanetocreateaphasewithintermediatepolarityforseparationofextremelyhydrophobiccompoundsusingconventionalreversedphasesolventsandofhighlypolarcompoundsusingnormalphasesolvents.

ThechemistryofYMC-PackTMS(C1)isalsowell-suitedfortheanalysisofmultifunctionalcom-pounds.SelectivitycharacteristicsofaC1bondedphasecanbeunique,andsamplesmustbetestedtodeterminethesuitabilityofthephase.

YMC-PackTMS(C1)isalsoavailableinpreparativeparticlesizes.

- stationary phase with the lowest hydrophobicity among reversed phase packing materials

- intermediate polarity between normal phase silica and other alkyl bonded reversed phases

- for fast separations of highly hydrophobic compounds

- alternative to C18 for the separation of hydrophilic compounds

YMC-Pack TMS (C1)

YMC-Pack TMS Specification


USPL13

Reversed Phase 105


YMC-Pack TMS (C1)

Water-soluble vitamins* Water-soluble vitamins*

1: L-Ascorbicacid (VitaminC)

2: Oroticacid (VitaminB13)

3: Pyrroloquinolinequinone (PQQ)

4: PyridoxineHCl (VitaminB6)

5: Nicotinamide

1: p-Aminobenzoicacid (PABA)

2: Biotin (VitaminH)

3: Riboflavin (VitaminB2)

4: Cyanocobalamin (VitaminB12)

Column care

YMC-PackTMS(C1)isstabletowardshydrolysisbetweenpH2.0-7.5.Removeacidandbuffersaltsbeforestorage.Storethecolumninmethanol/water=70/30.Ifcolumnsareaffectedbyundesiredcontaminantsorcloggedinletfritswhichcausebackpressureincreases,flushthecolumnwithTHFintheoppositeflowdirection.


510min 1020min

Column: YMC-PackTMS(C1)(5µm,12nm)150x4.6mmIDPartNo.: TM12S05-1546WTEluent: 100mMCH3COOH/100mMCH3COONH4(30/70,pH5.1)Flow: 1.0ml/minDetection: UVat254nm,0.16AUFSTemperature: 37°CInjection: 10µl(0.04~0.20mg/ml)

Column: YMC-PackTMS(C1)(5µm,12nm)150x4.6mmIDPartNo.: TM12S05-1546WTEluent: acetonitrile/50mMNH4H2PO4(10/90)Flow: 1.0ml/minDetection: UVat210nm,0.16AUFSTemperature: 37ºCInjection: 10µl(0.02~0.30mg/ml)

106 Reversed Phase

YMC-Pack CN (Cyano)

General

Inreversedphasemode,cyano(nitrile)phasesarethemostpolarandleastretentiveofallreversedphasesupports.Extremelyhydrophobiccompounds,whichdonoteluteonstandardC18andC8columnswithtypicalreversedphaseeluents,canbeseparatedusingcyanophases.SeparationsusingreversedandnormalphaseandHILICmechanismscanbecarriedoutusingthismaterial.

Properties

ThecyanochemistryofYMC-PackCN(Cyano)providesadifferentselectivityfrombothphenylandstandardaliphatic(C18,C8orC4)reversedphases.Itisusefulforquickandsimpleanalysisofcompoundsthatdiffergreatlyinhydrophobicity,withouttheneedtousegradientelutionchro-matography.

Cyanopackingsalsoprovideanalternativetosilicamaterialinnormalphasechromatography,wherebondednormalphasepackingshavetheadvantageoffasterequilibration,moreuniformsurfaceactivityandincreasedresistancetodissolution.

Toextendcolumnlifetimecontinuedswitchingbetweennormalandreversedphasesolventsshouldbeavoided.

YMC-PackCN(Cyano)isalsoavailableinpreparativeparticlesizes.

- for normal, reversed phase and HILIC applications

- silica gel with cyanopropyl groups

- faster column equilibration than normal silica gel

- most polar reversed phase column

YMC-Pack CN Specification


USPL10

Reversed Phase 107


YMC-Pack CN (Cyano)

Column: YMC-PackCN(Cyano)(5µm,12nm)250x4.6mmIDPartNo.: CN12S05-2546WTEluent: acetonitrile/water/TFA(15/85/0.05)Flow: 0.5ml/minDetection: UVat220nm,0.64AUFSTemperature: 30ºCInjection: 5µl

Aspartame in a soft drink*

Column care

YMC-PackCN(Cyano)isstabletowardshydrolysisbetweenpH2.0-7.5.Removeacidandbuffersaltsbeforestorage.Fordetailedinformationpleaserefertothe“ColumnCareandUseInstructions”whichareshippedwitheachanalyticalcolumn.

Column: YMC-PackCN(Cyano)(5µm,12nm)250x4.6mmIDPartNo.: CN12S05-2546WTEluent: acetonitrile/water(40/60)Flow: 0.6ml/minDetection: UVat254nm,0.32AUFSTemperature: 30°CInjection: 3µl(0.25mg/ml)

Steroids*

1. Caffeine 2. Aspartama 3. Benzoicacid

1020min

108 Reversed Phase

Ordering Information

Phase dimension

Column ID

[mm]Column length [mm]

Guard cartridges*with 10 mm length

[pack of 5]

30 (WT) / 33 (QT) 50 100 150 250

12 nm3 µm

2.1 AQ12S03-H3Q1QT AQ12S03-05Q1QT AQ12S03-10Q1QT AQ12S03-15Q1QT AQ12S03-25Q1QT AQ12S03-01Q1GC3.0 AQ12S03-H303QT AQ12S03-0503QT AQ12S03-1003QT AQ12S03-1503QT AQ12S03-2503QT AQ12S03-0103GC4.0 AQ12S03-H304QT AQ12S03-0504QT AQ12S03-1004QT AQ12S03-1504QT AQ12S03-2504QT AQ12S03-0104GC4.6 AQ12S03-0346WT AQ12S03-0546WT AQ12S03-1046WT AQ12S03-1546WT AQ12S03-2546WT AQ12S03-0104GC

20 nm3 µm


12 nm5 µm


20 nm5 µm


YMC-Pack ODS-AQ

Phase dimension

Column ID



[pack of 5]

30 (WT) / 33 (QT) 50 100 150 250

12 nm3 µm

2.1 AA12S03-H3Q1QT AA12S03-05Q1QT AA12S03-10Q1QT AA12S03-15Q1QT AA12S03-25Q1QT AA12S03-01Q1GC3.0 AA12S03-H303QT AA12S03-0503QT AA12S03-1003QT AA12S03-1503QT AA12S03-2503QT AA12S03-0103GC4.0 AA12S03-H304QT AA12S03-0504QT AA12S03-1004QT AA12S03-1504QT AA12S03-2504QT AA12S03-0104GC4.6 AA12S03-0346WT AA12S03-0546WT AA12S03-1046WT AA12S03-1546WT AA12S03-2546WT AA12S03-0104GC

20 nm3 µm


12 nm5 µm


20 nm5 µm


30 nm5 µm


YMC-Pack ODS-A

Phase dimension

Column ID



[pack of 5]

30 (WT) / 33 (QT) 50 100 150 250

12 nm3 µm

2.1 AM12S03-H3Q1QT AM12S03-05Q1QT AM12S03-10Q1QT AM12S03-15Q1QT AM12S03-25Q1QT AM12S03-01Q1GC3.0 AM12S03-H303QT AM12S03-0503QT AM12S03-1003QT AM12S03-1503QT AM12S03-2503QT AM12S03-0103GC4.0 AM12S03-H304QT AM12S03-0504QT AM12S03-1004QT AM12S03-1504QT AM12S03-2504QT AM12S03-0104GC4.6 AM12S03-0346WT AM12S03-0546WT AM12S03-1046WT AM12S03-1546WT AM12S03-2546WT AM12S03-0104GC

12 nm5 µm

2.1 AM12S05-H3Q1QT AM12S05-05Q1QT AM12S05-10Q1QT AM12S05-15Q1QT AM12S05-25Q1QT AM12S05-01Q1GC3.0 AM12S05-H303QT AM12S05-0503QT AM12S05-1003QT AM12S05-1503QT AM12S05-2503QT AM12S05-0103GC4.0 AM12S05-H304QT AM12S05-0504QT AM12S05-1004QT AM12S05-1504QT AM12S05-2504QT AM12S05-0104GC4.6 AM12S05-0346WT AM12S05-0546WT AM12S05-1046WT AM12S05-1546WT AM12S05-2546WT AM12S05-0104GC

YMC-Pack ODS-AM

*Guardcartridgeholderrequired,partno.XPGCH-Q1



Reversed Phase 109



Phase dimension

Column ID



[pack of 5]

30 (WT) / 33 (QT) 50 100 150 250

12 nm3 µm

2.1 AL12S03-H3Q1QT AL12S03-05Q1QT AL12S03-10Q1QT AL12S03-15Q1QT AL12S03-25Q1QT AL12S03-01Q1GC3.0 AL12S03-H303QT AL12S03-0503QT AL12S03-1003QT AL12S03-1503QT AL12S03-2503QT AL12S03-0103GC4.0 AL12S03-H304QT AL12S03-0504QT AL12S03-1004QT AL12S03-1504QT AL12S03-2504QT AL12S03-0104GC4.6 AL12S03-0346WT AL12S03-0546WT AL12S03-1046WT AL12S03-1546WT AL12S03-2546WT AL12S03-0104GC

12 nm5 µm

2.1 AL12S05-H3Q1QT AL12S05-05Q1QT AL12S05-10Q1QT AL12S05-15Q1QT AL12S05-25Q1QT AL12S05-01Q1GC3.0 AL12S05-H303QT AL12S05-0503QT AL12S05-1003QT AL12S05-1503QT AL12S05-2503QT AL12S05-0103GC4.0 AL12S05-H304QT AL12S05-0504QT AL12S05-1004QT AL12S05-1504QT AL12S05-2504QT AL12S05-0104GC4.6 AL12S05-0346WT AL12S05-0546WT AL12S05-1046WT AL12S05-1546WT AL12S05-2546WT AL12S05-0104GC

YMC-Pack ODS-AL

Phase dimension

Column ID



[pack of 5]

30 (WT) / 33 (QT) 50 100 150 250

6 µm

2.1 PC99S06-H3Q1QT PC99S06-05Q1QT PC99S06-10Q1QT PC99S06-15Q1QT PC99S06-25Q1QT PC99S06-01Q1GC3.0 PC99S06-H303QT PC99S06-0503QT PC99S06-1003QT PC99S06-1503QT PC99S06-2503QT PC99S06-0103GC4.0 PC99S06-H304QT PC99S06-0504QT PC99S06-1004QT PC99S06-1504QT PC99S06-2504QT PC99S06-0104GC4.6 PC99S06-0346WT PC99S06-0546WT PC99S06-1046WT PC99S06-1546WT PC99S06-2546WT PC99S06-0104GC

YMC-Pack PolymerC18

Phase dimension

Column ID



[pack of 5]

30 (WT) / 33 (QT) 50 100 150 250

12 nm3 µm

2.1 OC12S03-H3Q1QT OC12S03-05Q1QT OC12S03-10Q1QT OC12S03-15Q1QT OC12S03-25Q1QT OC12S03-01Q1GC3.0 OC12S03-H303QT OC12S03-0503QT OC12S03-1003QT OC12S03-1503QT OC12S03-2503QT OC12S03-0103GC4.0 OC12S03-H304QT OC12S03-0504QT OC12S03-1004QT OC12S03-1504QT OC12S03-2504QT OC12S03-0104GC4.6 OC12S03-0346WT OC12S03-0546WT OC12S03-1046WT OC12S03-1546WT OC12S03-2546WT OC12S03-0104GC

20 nm3 µm


12 nm5 µm


20 nm5 µm


30 nm5 µm


YMC-Pack C8

Phase dimension

Column ID



[pack of 5]

30 (WT) / 33 (QT) 50 100 150 250

12 nm3 µm

2.1 PH12S03-H3Q1QT PH12S03-05Q1QT PH12S03-10Q1QT PH12S03-15Q1QT PH12S03-25Q1QT PH12S03-01Q1GC3.0 PH12S03-H303QT PH12S03-0503QT PH12S03-1003QT PH12S03-1503QT PH12S03-2503QT PH12S03-0103GC4.0 PH12S03-H304QT PH12S03-0504QT PH12S03-1004QT PH12S03-1504QT PH12S03-2504QT PH12S03-0104GC4.6 PH12S03-0346WT PH12S03-0546WT PH12S03-1046WT PH12S03-1546WT PH12S03-2546WT PH12S03-0104GC

12 nm5 µm


30 nm5 µm







110 Reversed Phase


Phase dimension

Column ID



[pack of 5]

30 (WT) / 33 (QT) 50 100 150 250

12 nm3 µm

2.1 TM12S03-H3Q1QT TM12S03-05Q1QT TM12S03-10Q1QT TM12S03-15Q1QT TM12S03-25Q1QT TM12S03-01Q1GC3.0 TM12S03-H303QT TM12S03-0503QT TM12S03-1003QT TM12S03-1503QT TM12S03-2503QT TM12S03-0103GC4.0 TM12S03-H304QT TM12S03-0504QT TM12S03-1004QT TM12S03-1504QT TM12S03-2504QT TM12S03-0104GC4.6 TM12S03-0346WT TM12S03-0546WT TM12S03-1046WT TM12S03-1546WT TM12S03-2546WT TM12S03-0104GC

12 nm5 µm


30 nm5 µm


YMC-Pack TMS

Phase dimension

Column ID



[pack of 5]

30 (WT) / 33 (QT) 50 100 150 250

12 nm3 µm

2.1 CN12S03-H3Q1QT CN12S03-05Q1QT CN12S03-10Q1QT CN12S03-15Q1QT CN12S03-25Q1QT CN12S03-01Q1GC3.0 CN12S03-H303QT CN12S03-0503QT CN12S03-1003QT CN12S03-1503QT CN12S03-2503QT CN12S03-0103GC4.0 CN12S03-H304QT CN12S03-0504QT CN12S03-1004QT CN12S03-1504QT CN12S03-2504QT CN12S03-0104GC4.6 CN12S03-0346WT CN12S03-0546WT CN12S03-1046WT CN12S03-1546WT CN12S03-2546WT CN12S03-0104GC

12 nm5 µm


30 nm5 µm


YMC-Pack CN (Cyano)

Phase dimension

Column ID



[pack of 5]

30 (WT) / 33 (QT) 50 100 150 250

3 µm

2.1 BA99S03-H3Q1QT BA99S03-05Q1QT BA99S03-10Q1QT BA99S03-15Q1QT BA99S03-25Q1QT BA99S03-01Q1GC3.0 BA99S03-H303QT BA99S03-0503QT BA99S03-1003QT BA99S03-1503QT BA99S03-2503QT BA99S03-0103GC4.0 BA99S03-H304QT BA99S03-0504QT BA99S03-1004QT BA99S03-1504QT BA99S03-2504QT BA99S03-0104GC4.6 BA99S03-0346WT BA99S03-0546WT BA99S03-1046WT BA99S03-1546WT BA99S03-2546WT BA99S03-0104GC

5 µm

2.1 BA99S05-H3Q1QT BA99S05-05Q1QT BA99S05-10Q1QT BA99S05-15Q1QT BA99S05-25Q1QT BA99S05-01Q1GC3.0 BA99S05-H303QT BA99S05-0503QT BA99S05-1003QT BA99S05-1503QT BA99S05-2503QT BA99S05-0103GC4.0 BA99S05-H304QT BA99S05-0504QT BA99S05-1004QT BA99S05-1504QT BA99S05-2504QT BA99S05-0104GC4.6 BA99S05-0346WT BA99S05-0546WT BA99S05-1046WT BA99S05-1546WT BA99S05-2546WT BA99S05-0104GC

YMCbasic

For other dimensions please refer to page 247




Reversed Phase 111


reversed phase lq

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