restriction enzymes

Describe digestion of DNA by Learning Outcomes: 1. Define and Give examples of Restriction Endonucleases. 2. Explain how restriction enzymes produce Recombinant DNA. DNA Manipulation:

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Restriction Enzymes

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Describe digestion of DNA by

LearningOutcomes:1.DefineandGiveexamplesofRestrictionEndonucleases.

2.ExplainhowrestrictionenzymesproduceRecombinantDNA.

DNA Manipulation:

Enzymescalledrestrictionendonucleasesrevolutionisedmolecularbiologybecauseoftheirabilityto“cut”DNAatspecificsites.

ButHowDOrestrictionenzymeswork?

Theyallowthecreationofrecombinantmolecules;theseenzymesrecogniseaspecificDNAsequence,rangingfromandcuttheDNAataspecificbasewithinthissequence.

TheRecognitionsitesformanyRestrictionEnzymesarepalindromes...

Apalindromeisawordorphrasethatreadsthesameforwardandinreverse,e.g...

SO!...ThepalindromicDNAsequencereadsthesamefrom5'to3'ononestrand

asitdoesonthecomplementarystrand.

CuttingtheDNAatthesamebaseoneitherstrandproducesstaggeredcutsCalled“stickyends.”

SO,These“stickyends”allowDNAfromdifferentsourcestobeeasilyjoinedtogether!

Theseshort,unpairedsequencesarethesameforanyDNAthatiscutbythisenzyme...

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