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real-time rt-pcr

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We investigated the association of LTR element in trans cript variant of MOBP gene. LTR33 belonging to the ERV1 fami ly contributes to the change of coding region of MOBP gene b y te production of splicing variant. Real-time RT-PCR analys is using different human tissues shows the dominant expressi on patterns in AD brain, adult whole brain, spinal cord, and testis tissues. Interestingly, comparative analysis of expre ssion pattern using corresponding tissues between human and rhesus monkey shows the different expression patterns. To in vestigate the evolutionary conservation of LTR33 element of the MOBP gene, various primate DNA samples were used for the PCR amplification and sequencing. Their splicing donor site (GT) are completely conserved and the number of tandem repea ted element which could be translated are increased during t he evolution of hominoid primates. Taken together, integrati on event of LTR33 element could affect the coding capacity a nd expression in different tissues by changing the coding se quences during the primate evolution. Real-time RT-PCR PCR INTRODUCTION 0 0.002 0.004 0.006 0.008 0.01 0.012 0.014 0.016 0.018 alzheimer adult brain fetalbrain heart lung spinal cord testis thyroid trachea MOBP_LTR33/GAPDH 0 0.005 0.01 0.015 0.02 0.025 0.03 parietallobe medulla oblongata pons occipitallobe cereberal cortex corpus callosum olfactory thalamus frontallobe temporallobe hippocampus RESULTS & DISCUSSION MERERIALS & METHODS REFERENCES www.primate.or.kr Dual coding capacity of MOBP gene derived by the Dual coding capacity of MOBP gene derived by the insertion of retroviral LTR33 element insertion of retroviral LTR33 element Yu-Na Noh Yu-Na Noh 1 1 , Jae-Won Huh , Jae-Won Huh 1 1 , Dae-Soo Kim , Dae-Soo Kim 2 2 , Kung Ahn , Kung Ahn 1 1 , and Heui-Soo Kim , and Heui-Soo Kim 1,2 1,2 1.Division of Biological Sciences, College of Natural Sciences, Pusan National University 1.Division of Biological Sciences, College of Natural Sciences, Pusan National University 2.PBBRC, Interdisciplinary Research Program of Bioinformatics, College of Natural Sciences, Pusan 2.PBBRC, Interdisciplinary Research Program of Bioinformatics, College of Natural Sciences, Pusan National University National University ABSTRACT [Structure of Retrovirus] [Retrovirus life cycle] 8% [Genome component parts] [HERV Structure] *HERV: Human Endogenous Retrovirus Gene Cloning Bio Informatics Primate DNA MOBP Gene Human Gorilla Orangutan Gibbon Old World monkeys New World monkeys Prosimians Million years 70 60 50 40 30 20 10 LTR33 [Figure 5. Real-time RT-PCR analysis of MOBP gene transcript] [Figure 6.Integration lineage of LTR33 element during the Primate evolution.] Amplified Plasmid DNA 1.Yoichi Yamamoto et al. 1994,Cloning and Expression of Myelin-a ssociated Oligodendrocytic Basic Protein, The Journal of Biological Chemistry,Vol.269:31725-31730 2. HOLZ A. and SCHWAB M. E.,1997,Developmental Expression of the myelin gene MOBP in the rat nervous system, Journal of Neurocytology,26:467-477 [Figure 4.Comparative analysis of MOBP gene transcripts between Human and Rhesus Monkey] [Figure 1. Four different alternative transcripts of MOBP gene] [Figure 3. Expression analysis of alternative transcripts in various human tissues including different brain tissues.] [Figure 2. Multiple alignment of MOBP gene.]

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[Figure 3. Expression analysis of alternative transcripts in various human tissues including different brain tissues.]. 8%. Human. Real-time RT-PCR. Gorilla. Orangutan. LTR33. Gibbon. Old World monkeys. New World monkeys. Prosimians. Million years. - PowerPoint PPT Presentation

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Page 1: Real-time RT-PCR

We investigated the association of LTR element in transcript variant of MOBP gene. LTR33 belonging to the ERV1 family contributes to the change of coding region of MOBP gene by te production of splicing variant. Real-time RT-PCR analysis using different human tissues shows the dominant expression patterns in AD brain, adult whole brain, spinal cord, and testis tissues. Interestingly, comparative analysis of expression pattern using corresponding tissues between human and rhesus monkey shows the different expression patterns. To investigate the evolutionary conservation of LTR33 element of the MOBP gene, various primate DNA samples were used for the PCR amplification and sequencing. Their splicing donor site (GT) are completely conserved and the number of tandem repeated element which could be translated are increased during the evolution of hominoid primates. Taken together, integration event of LTR33 element could affect the coding capacity and expression in different tissues by changing the coding sequences during the primateevolution.

Real-time RT-PCR

PCR

INTRODUCTION

0

0.002

0.004

0.006

0.008

0.01

0.012

0.014

0.016

0.018

alzheimer adultbrain

fetal brain heart lung spinalcord

testis thyroid trachea

MO

BP_L

TR

33

/GA

PD

H

0

0.005

0.01

0.015

0.02

0.025

0.03

parietal lobe medullaoblongata

pons occipital lobe cereberalcortex

corpuscallosum

olfactory thalamus frontal lobe temporal lobe hippocampus

RESULTS & DISCUSSION

MERERIALS & METHODS

REFERENCES

www.primate.or.kr

Dual coding capacity of MOBP gene derived by the Dual coding capacity of MOBP gene derived by the insertion of retroviral LTR33 elementinsertion of retroviral LTR33 element Yu-Na NohYu-Na Noh11, Jae-Won Huh, Jae-Won Huh11, Dae-Soo Kim, Dae-Soo Kim22, Kung Ahn, Kung Ahn11, and Heui-Soo Kim, and Heui-Soo Kim1,21,2

1.Division of Biological Sciences, College of Natural Sciences, Pusan National University 1.Division of Biological Sciences, College of Natural Sciences, Pusan National University 2.PBBRC, Interdisciplinary Research Program of Bioinformatics, College of Natural Sciences, Pusan National University2.PBBRC, Interdisciplinary Research Program of Bioinformatics, College of Natural Sciences, Pusan National University

ABSTRACT

[Structure of Retrovirus]

[Retrovirus life cycle]

8%

[Genome component parts][HERV Structure]

*HERV: Human Endogenous Retrovirus

Gene Cloning

BioInformatics

Primate DNA MOBP Gene

Human

Gorilla

Orangutan

Gibbon

Old World monkeys

New World monkeys

Prosimians

Million years

70 60 50 40 30 20 10

LTR33

[Figure 5. Real-time RT-PCR analysis of MOBP genetranscript]

[Figure 6.Integration lineage of LTR33 element during thePrimate evolution.]

AmplifiedPlasmid DNA

1.Yoichi Yamamoto et al. 1994,Cloning and Expression of Myelin-associated Oligodendrocytic Basic Protein, The Journalof Biological Chemistry,Vol.269:31725-317302. HOLZ A. and SCHWAB M. E.,1997,Developmental Expression of the myelin gene MOBP in the rat nervous system, Journal ofNeurocytology,26:467-477

[Figure 4.Comparative analysis of MOBP gene transcripts between Human and RhesusMonkey]

[Figure 1. Four different alternative transcripts of MOBP gene]

[Figure 3. Expression analysis of alternative transcripts in various human tissues including different braintissues.]

[Figure 2. Multiple alignment of MOBPgene.]

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