reagent #2 0.001m luminol in 0.1m naoh ph 12 , volume 100 µl
DESCRIPTION
Chemiluminescence :. Sequencing of Sample and Reagents. Holding Coil. Flow Cell. counts. LUMINOL. FIBER OPTICS. Fe. PRODUCT. STEP#1 REAGENT LOADED INTO FLOWCELL STEP#2 SAMPLE INJECTED INTO FLOWCELL. Reagent #2 0.001M luminol in 0.1M NaOH pH 12 , volume 100 µL - PowerPoint PPT PresentationTRANSCRIPT
Reagent #2 0.001M luminol in 0.1M NaOH pH 12,volume 100 µL Carrier: an aqueous solution containing 0.025% (v/v) Brij Sample Fe (II) in 0.001 N HCl volume 50 µL
Chemiluminescence:
STEP#1 REAGENT LOADED INTO FLOWCELL STEP#2 SAMPLE INJECTED INTO FLOWCELL
Sequencing of Sample and Reagents
Hugo Oliveira, unpublished results from N.B. Palmer cruise Antarctica 2011
y = 14.798x + 116.24
R2 = 0.9997
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Fe / nM
Peak
Hei
ght
counts
FIBER OPTICS
Flow CellHolding Coil
LUMINOL
Fe
PRODUCT