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Macquarie University Quarantine Standard Operating Procedures Last edited 25/10/11 by Elsa Mardones

Imported Goods Pathway of Quarantine Materials at Macquarie University.

Quarantine Approved Facility

(QAP QC2)

E8A109

INCOMING SOURCES

-Antarctic Division

-Interstate and Overseas

Universities

- Others

-Permit to import (AQIS) Couriers used:

World Courier

DHL

FEDEX

Samples received,

checked and stored by

Quarantine manager

Processing of

samples by AQIS

certified personnel

only

Waste Disposal

After treatment

Released samples

with AQIS approval

after appropriate

treatment

Co-locations: E5A052 &

034(Store), E7B345 &

E7B340/344

QAP QC1

1

Contents Page #

1. Dealing with Quarantine Materials at Macquarie University 2

2. Introduction 3

3. Procedures to follow 4-5

4. Clean up in event of spills 6

5. Pest control 7

6. Maintenance of equipment 7

7. Personal protective equipment & personal disinfection 8

8. Transfer procedure to co-located facilities 8

9. Waste disposals, solids and liquids 8

10. Flood risk procedures 8

11. Safe working procedures Organic Geochemistry lab. E7B340/344 9-11

12. Safe working procedures Luminescent lab E7B345 12-14

13. Checklist for Quarantine Lab. QC2 15-18

Attachment 1 Broad spectrum disinfectants for treatment where

prescribed under Class 5 Quarantine Approved Premises Criteria

Attachment 2 Quarantine pesticide application logbook

Attachment 3 Sample Information logbook

Attachment 4 Map of campus indicated Co-located facility and

nearest available parking.

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1. - How to deal with Quarantine Materials at Macquarie University

1.1 Before import, determine quarantine status. If your material is not subject to quarantine, do not import

under quarantine permit. Check with AQIS to establish if the proposed importation requires a Permit to Import

Biological Materials. (www.aqis.gov.au/icon/)

1.2 If a Permit to import is required, inform the University’s Quarantine Manager of your plans, and remember

to provide copies of all related documentation.

1.3 Go to www.daff.gov.au/aqis then:

Importing to Australia

↓

Application to import Quarantine Material

↓

Guide to submitting your Application

↓

Download Application form → Apply online→ fees apply

1.4 Once a permit has been obtained from AQIS, a copy must be provided to the Universities AQIS Manager.

1.4.1 A copy of the permit, delivery instructions, address and Quarantine Material labelling needs to

be passed on to the sender.

1.4.2 Copies of the permit and Quarantine entry must be attached to all boxes delivered to the

University.

1.4.3 Couriers should be advised by the sender about the contents.

1.4.4 An information sheet with the required details can be obtained from the University AQIS

Manager or Research Office website under the quarantine material section. An example of such information

is shown in this document.

1.5 With AQIS permit on hand organise your material, addressed to our Quarantine Approved Facility E8A

109, attention to Elsa Mardones

1.6 As soon as parcel arrived, it will be place in E8A109 in Fridge, Freezer or at room Tº in an AQIS

approved facility, E8A109.

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2. Introduction

All materials entering to Macquarie University under a quarantine permit are subject to stringent regulations; you

need to be aware of the following requirements before importing materials.

All applications to import quarantine materials must be signed off by the University’s Quarantine Officer. This is

currently held by Ms Elsa Mardones. This must be done prior to the application to AQIS, with copies made for

record keeping purposes. The reason for this is so the University knows what you are planning to bring on to the site,

where you intend to store it and what it will be used for. You must understand that if your actions regarding

importation or storage of samples contravene AQIS guidelines, and this is found on one of their bi-annual

inspections, you will be responsible for the shutdown of AQIS QAP (Quarantine Approved Premises) on Campus.

Quarantined materials can only be stored and worked upon within a QAP (Quarantine Approved Premises).

Currently, two laboratories constitutes QAP approved by AQIS, these are located in E8A 109 , E5A052 and

E034(only store).Quarantined material stored and worked on anywhere else within Macquarie University is in

contravention of the Quarantine Act.

Furthermore, quarantined material can only be worked on by an Accredited Person (who has done an AQIS

approved on-line training course http://www.iwgq.com.au or someone directly under the supervision of an accredited

person. Anyone else who is processing quarantined material is in breach of the Quarantine Act.

Access to QAP’s is therefore strictly controlled and the University Quarantine Officer Ms Elsa Mardones should be

contacted if key is required ext. # 8233 and Mr Russell Field ext # 8341 in her absence.

AQIS regulations place stringent responsibilities on supervisors of QAP to account for and control all quarantine

material within them.

To ensure we are working within AQIS regulations, and to minimise the laborious record-keeping required by AQIS,

it is to our advantage to ensure only materials which specifically need to be quarantined are imported under a

quarantine permit.

Determine the status of materials before import. If a material is not necessary to be quarantined ensure it is not

imported on a quarantine permit. Then it can be freely processed by anyone. For example, only waters, soils and

living materials from outside Australia and Antarctica need to be quarantined. Bare rock, mineral separates, drill core

probably do not need to be imported under a quarantine permit, and only AQIS can confirm it.

Paper or electronic records must be kept of the location and processing of all quarantined material (including

derivatives such as milled powders or diluted solutions etc) and is available for the inspection of AQIS inspectors.

Accredited persons cannot be expected to control materials which third parties have imported and intend to process

outside of QAP. Furthermore any processing must ensure that other materials are not contaminated with the

quarantined material, i.e. quarantined material must be kept separated from other materials.

Be aware that there are treatment processes which render quarantined material suitable for issue of a clearance

notice. Seek AQIS for clearance; do not assume treatment renders material safe.

Steritech Pty Ltd is an approved company by AQIS who does treatments to released materials from Quarantine, but

before the material is sent to them, you must contact AQIS, they provide you with an AQIS approved authority to

treat goods form, it is the only way you can removed your samples from QAP to Steritech.

If you need to transfer samples from QAP to another QAP it is necessary to inform AQIS, they will provide you with

the Application for transfer of quarantine material form, unless is specified in the import permit.

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3. Procedures to follow

1. Before import determine quarantine status. If your material is not subject to quarantine, do not import under

quarantine permit. Check with AQIS to establish if the proposed importation requires a Permit to Import Biological

Materials. (www.aqis.gov.au/icon/)

2. If your material is subject to quarantine, then import under quarantine permit, but ensure appropriate description

of material and appropriate permit conditions. If a permit is required then please see Elsa Mardones, who is the

Quarantine Officer for the Faculty of Science, she can give you a copy of the permit and give advice on importation

issues. Please read the permit carefully and comply with all permit conditions. This is most important to facilitate the

movement or clearance of the consignment. If you are unsure then please check with Elsa for advice.

A copy of the permit must accompany the consignment. If you do not want the consignment opened then arrange for

the permit to be placed in an envelope and stuck on the outside of the consignment. If the permit indicates that the

consignment requires an inspection address then consignment to:

Elsa Mardones – Quarantine Officer

Biology Store, Building E8A Faculty of Science Store,

Eastern Rd

Macquarie University, NSW 2109

It is most important for the consignment to have the end users name so that the carrier can charge you the fees for

service. It also identifies the end user so that quarantine and your department can make contact with you.

A quarantine entry will be created and inspection fees will be applied. It is not possible to define the fees, as they are

different for each particular service.

If you carry your consignment through one of the international airports of Australia declare it to quarantine and

present a copy of the permit to facilitate the process.

The consignment may be ‘Ordered into Quarantine’. This could mean that the consignment is taken from you and

sent under bond to the AQIS Regional Office for inspection, or, if the consignment is assessed as low risk you may

be allowed to carry it on a domestic flight. Please take note of the instructions on the ‘Order into Quarantine’. It is a

legal document and you are required to carry out the written instructions.

All consignments entering the University are to be notified to Elsa Mardones, Quarantine Officer, Faculty of

Science.

AQIS is required to audit all importations randomly. Elsa is required to have access to all relevant documentation.

These are the quarantine entry, carrier documentation, consignment order documentation and scientific research

documentation.

All items of possible quarantine concern received through the post should be referred to Elsa Mardones for possible

referral to AQIS.

3. Ensure a QAP is available to receive the material when it arrives. Ensure an accredited person is available to take

control of material when it arrives, and to handle administrative record-keeping.

4. When material arrives, place it within QAP and ensure all processing is confined within QAP and all materials

including derivative materials are accounted for.

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Records kept for imported goods should include –

- Location (Lab. # Fridge or Freezer)

- Sample number

- the date the material is received

- the quarantine entry number

- the permit number

- type of goods (a description of the materials)

- origin (where the samples come from)

- amount of sample/s received

- the proposed research and analysis details

- details of any special treatments

- the methods of disposal (30’@121°C if it is autoclaved)

- the amount of sample/s for disposal

- the dates of disposal

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4. Clean up in event of spills

The equipment used, such as brooms, dustpans, and double lined bins will remain within the facility. The applicable

method of cleaning of this equipment (via disinfectant, sterilisation, or other AQIS approved method) and the

spillage area will be undertaken with an AQIS approved broad-spectrum disinfectant as noted in the document

“Broad-spectrum disinfectants for treatment where prescribed under the Class 5 Quarantine Approved Premises

Criteria”.

On our premises we supply Decon 90 as a broad-spectrum disinfectant. Using gloves and safety glasses, cover the

contaminated area with Decon 90, leave it for few minutes and then pick up using the equipment provided for that

purpose, then deposit the waste in a double autoclave bag and carry it, in a well labelled (quarantine waste, name of

user, contact number) seal container to the autoclave located outside our QAP E8A108 or E8C202. All spillage or

loss of quarantine material will be immediately reported to the Faculty of Science AQIS laboratory manager.

SPILL MANAGEMENT INSTRUCTIONS

1. PUT ON RELEVENT GLOVES, EYEWEAR, MASK AND PLASTIC APRON

2. COVER AREA OF SPILL WITH ABSORBENT POWDER, STARTING AT THE OUTSIDE OF THE

SPILL TO CONTAIN FLUID. SPRINKLE GENEROUSLY ONTO THE REMAINDER OF THE SPILL.

ALLOW ABSORBENT POWDER TO CONGEAL THE SPILL (APROX 3-5 MINUTES)

3. MANY LABORATORY SPILLS INVOLVE BROKEN GLASS. THE SPILL RESPONDER MUST

AVOID GETTING CUT, REMOVE BROKEN GLASS WITH TONGS AND PLACE THEM INTO THE

PLASTIC CONTAINER (20 L BUCKET)

4. USING PLASTIC DUST PAN AND SCRAPER PICK UP THE SUBSTANCE AND PLACE IT INTO

AUTOCLAVE BAG, SEAL THE BAG TIGHTLY USING TIES PROVIDED.

5. WIPE AREA WITH ABSORBENT CLOTHS TO REMOVE ANY REMAINING CONTAMINANT.

6. DISCARD ALL USED ITEMS INTO AUTOCLAVE BAG AND SEAL. ORIGINAL CONTAINER WITH

SEALED LID MAY ALSO BE USED TO DISCARD SPILLS, PARTICULARLY IF BROKEN GLASS IS

PART OF THE SPILL

7. DISPOSE OF CONTAMINATED PRODUCT IN ACCORDANCE WITH STATE/ TERRITORY

REGULATIONS

8. WASH THE SPILL AREA WITH DECON 90

9. WASH HANDS THOROUGHLY AFTER CLEANING IS COMPLETED

10. REPORT USE OF KIT TO ELSA MARDONES ph 9850 8233 SO THAT A REPLACEMENT KIT CAN

BE ORGANISED.

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5. Pest Control

The current pest control procedure undertaken by the Faculty of Science follows once a month inspection regime.

Checking baits monthly and replacing baits every 3 months, looking for signs of any other pests. To prevent the

unauthorised removal of quarantined material or the escape of pest and disease organisms, the doors to the facility

are closed at all times while quarantine work is in progress. A document outlining the control measures and

inspection schedule is available to AQIS for audit purposes (an example of such a document is attached).

In case of infestation by pests, AQIS will be informed immediately by the on campus AQIS Manager. AQIS contact

no. 02 8334 7444.

6. Maintenance of equipment

In the Faculty of Science of Macquarie University, the autoclaves, biosafety cabinets and fume hoods are regularly

maintained and calibrated in accordance with NATA requirements. See attachment 4, calibration certificate.

Geoscience equipment maintenance is based around the cleaning procedures between samples to eliminate cross

contamination.

7. Personal Protective Equipment (PPE) & Personal Disinfection

Gloves, paper masks are disposed with AQIS designated waste according to their requirements. See section 9 below.

Lab coats are steam sterilised before being sent out for clean via a laundry service.

Hands disinfection: After completion of any activities regarding quarantine samples, it is compulsory to disinfect

the hands with chemicals approved by AQIS; Faculty of Science provides moisturising antibacterial hand gel with

ethyl alcohol (70%) and clorhexidine gluconate (0.5%) as the active ingredients.

8. Transfer Procedure –Co-located Facilities

Movement of samples from one AQIS facility to another supervised by the AQIS Manager will follow a procedure

where movement information is recorded in the sample log sheet. This provides safe and efficient movement from

one building to another. All samples moved from these premises should have their records updated to reflect their

current storage location. (See attachment 3). No samples are to be moved without the knowledge of the Universities

Quarantine Contact Officer.

Samples will be placed in a sealed plastic container. The container will then be placed in a large container and

transported by trolley to prevent accidentally dropping.

9. Waste Disposal, Solids and Liquids

Below is the AQIS criterion for disposal of waste. Please consult Elsa Mardones before undertaking disposal.

Records need to be kept and updated. These criteria are to be followed for all QAP facilities in the Faculty of

Science.

Autoclave booking See Elsa Mardones for Induction training of the autoclaves.

The autoclaves are situated in the E8C202 and a log book is placed inside of this room, at the moment of booking

you MUST write down Quarantine Waste in the Column “Type of Load” (See attachment 4).

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Please advise the AQIS manager when you wish to dispose of materials from the Co-located facility. Movement of

materials back to the Biology facility for disposal are to follow the Transfer procedures stated above. All records of

movement need to be updated during this process.

Solid quarantine wastes: - must be bagged and placed in an unbreakable container with a secured lid for movement

within or outside the building to the approved disposal place. Where waste cannot be disposed of immediately, there

must be as a minimum the provision for:

• A separate storage device/area for the temporary holding of goods;

• Storage in lidded bins/containers of an appropriate size which are leak and pest proof,

• Bins to be labelled ‘Quarantine Waste’, and

• Double bagging of all waste.

Note: The separate storage device/area must be AQIS approved and be within the QAP to prevent loss, spillage or

unauthorised access.

AQIS approved methods of solid quarantine waste disposal include incineration at a high temperature in a high

efficiency Environmental Protection Agency (EPA) approved incineration facility, deep burial, or sterilisation by

autoclaving. For access to the autoclave, please see Elsa Mardones. Minimum autoclaving times after attainment of

temperature for all goods, residues or quarantine waste shall be 121°C for 30 minutes

Quarantine waste water -

AQIS approved methods include disposal of waste water by an approved municipal sewage system. The use of other

waste disposal methods must be approved in writing by AQIS after demonstration of their efficacy to the satisfaction

of AQIS. Such methods may require detailed scientific research at the QAP holders’ expense. Additionally, all

quarantine waste water disposal must comply with requirements stated by:

• The State/Territory Environmental Protection Agency,

• Local Council, and

• The Water Board (if applicable).

10. Flood risk Procedures

According to one in hundred years flooding certificate obtained from City of Ryde Council, Macquarie University

has the risk of flooding, if this event occurs of either co-located facility, the following steps are to be taken in order

to protect the integrity of the goods in quarantine,

1) Contact the Universities Quarantine Contact Officer Elsa Mardones and the Faculty of Science Infrastructure

Manager, Ron Claassens.

2) Operating under the direction of the Quarantine Contact Officer, move all affected samples, fridges, or

freezers, to the other on-campus co-located facility or an allocated storage room until all cleared. Movement

of samples should follow the transfer procedure as stated above.

3) Ensure all relevant documentation is updated to reflect the movement of samples.

If in the event that the entire University were flooded, the following procedures should be followed:

The Universities Quarantine Contact Officer Elsa Mardones and the Faculty of Science Infrastructure Manager, Ron

Claassens would make a determination regarding location of a safe space in one the multi story buildings that the co-

located facilities are housed in. This would comprise a higher floor laboratory or room that could contain the

samples, fridges and freezers and would have access to it restricted until such time as the disaster was over and the

original QAP was made available for use

Restrictions:

Safety goggle

laborator

Hazard Possible

Spillage Non-quarantine samples a

contamin

from labora

Environment: Organic Geochemist

Safe

Organic Geochemist

Faculty: Science Department: Ea

Contacts: A/Prof Simon Geo

Approvals, Training or Certific

material must have “Accredit

Supervision required: None on

Personal Protective

Equipment:

Potential Hazards:

Do not eat or drink in labora

Quarantine and non

9

ety goggles, lab coats, and closed footw

laboratory

Controls

quarantine samples are Samples stored sepa

taminated and removed and processed sepa

om laboratory by accredi

Organic Geochemistry Laboratory Quarantine facility

Working Procedures

Use of Quarantine Samples in

Organic Geochemistry Laborato

E7B 340 / 344

tment: Earth and Planetary Science

of Simon George x4424 (0418428217)

ertifications required: All personnel working with Quarantine

ted Person” certificates from AQIS

None once approval has been given

Do not eat or drink in laboratory, store or mix

Quarantine and non-Quarantine samples.

wear must be worn in the

ols Consequence

ed separately

essed separately

edited people

y Quarantine facility

tine Samples in

y Laboratory

(0418428217)

orking with Quarantine

10

Specific

Transport: Samples are to appropriately labelled and transported on a trolley in a well-labelled

plastic container to the isolated Quarantine facility.

Procedures: All samples transported to this quarantine facility should have their records updated to

reflect their current storage location. No samples are to be moved without the

knowledge of the quarantine manager (Elsa Mardones)

Storage: Samples are to be stored in the alarmed isolated freezer in the laboratory labelled

“Quarantine”. Samples are not to be stored with non-Quarantine samples. No Quarantine

materials will be stored anywhere else.

Hygiene: Glass vials and pipettes that come into contact with Quarantine material will be stored

in the labelled Quarantine glass waste repository until disposed of, or will be heated to

>150 degrees C in a furnace or oven.

Movement

between QAPs: No Quarantine material will be removed from the laboratory without written permission

by AQIS unless is specified in the import permit, any movement must provide details to the

quarantine manager

Waste

Management: Wastewater will be autoclaved in a secure and labelled container until waste removal

(see movement between QAPs). Quarantine waste will be collected only by Elsa

Mardones and disposed of by an AQIS -approved waste collection and transport

company, or treated by AQIS approved methods.

QAP

accredited

persons &

procedures: Only QAP Accredited Persons will carry out processing and analysis of quarantine

materials. Processing will include: (1) De-frosting of frozen samples (this will only be

carried out in fume cupboard 1 (FC1), and no other lab operations will take place in this

same FC1 at the same time. (2) Decanting of water into new, clean glass vials for analysis.

Dilution of de-frosted water samples with de-ionised water. (3) Analysis of diluted

quarantine materials on the purge-and-trap GC-MS. When this is being used to analyse

quarantine materials, no other samples will be analysed at the same time. Wastewater

from the system will be collected as detailed in Waste Management.

Identification

& Traceability: All Quarantine material will be clearly labelled and a record kept in a spreadsheet of all

the samples in the lab. This will be kept for >18 months after the last sample is removed

from the lab. This record will include date of receipt of samples, container number,

volume of sample, dates of processing, dates of analysis, storage location, disposal

details. Simon George will be responsible for all items.

Awareness: All people normally working in the lab who are not QAP trained, and anybody visiting

the lab for whatever reason, have to be trained by a QAP Accredited Person. This training

will include:

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1. Where the storage facility for the quarantine materials is located (freezer in E7B 344;

end one near wall).

2. That they must not touch or remove any quarantine materials from the lab, including

quarantine wastes. These will be clearly labelled.

3. That they must not interfere with any experiments or processes being carried out on

quarantine materials by a QAP trained person. When experiments are being carried out

on quarantine materials in fume cupboard 1 (FC1), no other lab operations will take

place in this same FC1. When the purge and-trap GC-MS is being used to analyse

quarantine materials, no other samples will be analysed at the same time.

Contingency

Plan: Spillage of quarantine material. The water will be collected and any materials used to

contain the spill will be retained as quarantine waste. AQIS will be notified immediately

by Elsa Mardones. If Simon George is unavailable to deal with an AQIS matter in the lab,

then Sargent Bray should be contacted. Failing that, Elsa Mardones should be contacted.

If there are any appearance of pests or symptoms of disease, or structural damage of the

lab, or apparent unauthorised removal of quarantine material, then AQIS will be notified

immediately

Clean Up &

Waste

Disposal: All surfaces are to be cleaned with Decon-90 after processing. Quarantine wastes are

placed in designated Quarantine bins. Quarantine waste is to be taken to building E8C

and autoclaved and disposed down a sink. Glassware is to be removed as specified in

Waste Management.

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Safe Working Procedures

USE OF QUARANTINE AND

NON-QUARANTINE SAMPLES

IN LUMINESCENCE

LABORATORY E7B345 Safe Working Procedure No: 1 Version: 3

Faculty: SCIENCE Department/Office

:

ENVIRON AND

GEOG

Contact(s): Dr Kira Westaway 8429 (0424285977)

Location of

Equipment

Approvals, Training

or Qualifications

required:

All personnel working with Quarantine material must have ‘Accredited

Person’ certificates from AQIS

Supervision Required Not once approval has been given

Restrictions (Insert symbol & wording)

Do not: Eat or drink in laboratory, store or mix Quarantine and non-Quarantine samples

Personal Protective

Equipment (Insert PPE symbol and

wording )

Safety glasses, lab coat, heat resistant gloves, enclosed footwear

Potential Hazards Hazard Possible

Consequences

Controls

Mixing of samples Non-quarantine

samples are

contaminated and

then removed from

lab

Samples are stored

and processed

separately by

accredited people

Environment Luminescence laboratory Quarantine facility – in subdued red light conditions

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Procedure

SPECIFIC PROCEDURE: Transport to the laboratory: Once subject to Quarantine samples are to be transported to the laboratory and Quarantine facility by the quickest and most direct route possible. Storage: Quarantine samples are to be stored in boxes labeled with signs provided (“Goods subject to Quarantine”) in the Quarantine storage room in the centre of the wet lab (containing “Quarantine storage” sign on door). Non-quarantine samples will be stored separately in the cupboards under the benches in the wet lab. Processing: Non-quarantine samples: are to be opened on the long bench by the door – once placed in beakers all processing operations such as sieving are conducted at a different time than the users processing the Quarantine samples. For use of the fume hood – the non-Quarantine samples are placed on the far right hand side. All surfaces and fume hood are cleaned with Decon-90 after use and the floor is sweep and mopped to avoid cross contamination. Quarantine samples: are to be opened on the balance table and will

be placed in the centre of the fume hood. Quarantine samples DO NOT leave this facility without undertaking an AQIS approved treatment. As a general rule samples are seldom removed from the laboratory as the samples are light sensitive so light exposure would cause them to be destroyed. However, on the rare occasion that non-Quarantine samples must be removed for processing in another laboratory then checks will be made to ensure that these guidelines have been followed.

Clean Up & Waste

Disposal

All surfaces are to be cleaned with Decon-90 after processing – especially after sample opening and sieving. All glassware is soaked in Decon-90 overnight before being cleaned and dried. The floors are sweep and mopped after the opening and sieving procedures and at the end of every day. Quarantine waste (sample wrappings, paper towel and filter paper) is placed in designated Quarantine bins and non-Quarantine waste is placed in the unmarked bin. The Quarantine waste is sealed, labelled and taken to E8C202 to be autoclaved (see map). In the unlikely event that Quarantine sediment needs to be removed from the lab after processing and measurement – the sediment must be treated in the oven with dry heat at 160°C for 2

hours (an approved AQIS treatment) AQIS must be immediately informed of any incidents which could significantly compromise the quarantine security of the facility. This may include structural damage, electrical breakdowns, escapes or unauthorised entry and the removal of quarantine material.

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Date Approved

Date of Commencement

Date for Next Review

Supporting Documentation

Relevant Legislation,

Guidelines and Standards

15

CHECKLIST for Quarantine facility QC2 N0100(Compiled by E. Mardones 16/09/ 2011) General requirements

Yes No Notes/Details

Hygiene and Isolation

Facility Fully enclosed space bounded by walls, doors, windows, floors and ceilings

Doors and windows

Any doors or windows able to be opened are lockable

Windows that are able to be opened are screened to prevent entry and exit of arthropods

Floors and benches

Cleanable, easily decontaminated and resistant to damage by cleaning agents and/or disinfectants to be used in the facility

B) Security

Doors must be closed when quarantine work is in progress and/or when quarantine goods are being held in the facility

Name and phone numbers of the facility manager is display near access doors

Quarantine sign is displayed on the entry door to the facility

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Operational procedures Yes No Notes/Details

Containers holding quarantine goods are clearly labelled, with following information: Quarantine entry Number, Import Permit, Importation date and contact details

Equipment must be disinfected routinely or before service by AQIS approved broad spectrum disinfectant

Gloves must be removed and hands thoroughly washed before leaving the facility. Gloves shall be discarded with quarantine waste.

To prevent cross contamination while work is being undertaken, there must be separation of quarantine work from other work

When working with quarantine goods,

personnel must wear covering clothes

closed footwear and

dirty clothing must be removed and laundered before re-used

Standards operating procedures must be available within the quarantine facility

Provide calibration and certification of sterilisers, and the efficiency of treatment

If biological safety cabinet is used to quarantine functions, the cabinet must be annually tested by qualified technician

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Waste disposal procedures Yes No Notes/Details

Quarantine waste is effectively contained and disposed of in a manner approved by AQIS

Administration and management

Records requirements:

Quarantine Entry Number(where relevant)

Import Permit Number

Description of the regulated goods(using scientific terminology)

Date of receipt of Goods and country of origin

Location where the quarantine item is held

Records of any derivatives and additional cultures/material grown from original material

Quantity of good/s received, destroyed and in storage

Date of completion of research

Detail of any treatment

Method and date of disposal/destruction

Date and the AQIS permission for any movement of quarantine goods

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Comprehensive details of any breaches of quarantine goods from the facility

Capacity to comply Yes No Notes/Comments

Demonstrated capacity to comply with the conditions of certification, that will generally be applied to a certified QC2 Facility for microbiological containment such as:

-summary of records must be provided at audit or at the request of an AQIS Officer

-A logbook recording steriliser load, temperature and duration of sterilisation cycle must be maintained.

- Calibration specifications for all equipment used for quarantine purpose must be recorded and provided at audit or at request of an AQIS Officer.

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Attachment 1

Broad-Spectrum Disinfectants for Treatment Where Prescribed Under the Class 5 Quarantine Approved Premises

Criteria

If animal goods are used disinfection treatment can occur with an AQIS approved disinfectant such as Virkon, Phoraid or Sodium Hydroxide.

If plant goods are used disinfection treatment can occur with an AQIS approved disinfectant such as Virkon, Decon 90 or Biogram

If microbiological goods are used disinfection treatment can occur with an AQIS approved disinfectant such as Virkon.

Quarantine Treatments (Correct as of 1 October 2005)

Chemical Solution Type Treatments

Some of the disinfectants effective for use at a Class 5 Containment Facility are outlined in the table below.

Disinfectant Rate

Virkon disinfectant

Virucidal Disinfectant:

The commercial formulation is Virkon®S, a peroxygen based

on potassium peroxomonosulphate.

As per manufacturer's directions.

20% Phoraid disinfectant

The commercial formulation is Phoraid®.

20% solution (200ml per 1L).

Sodium hydroxide - (Caustic soda, NaOH):

2% solution.

Add 1kg of caustic soda to 50L of water.

Decon 90 As per manufacturer's directions.

Biogram As per manufacturer's directions.

20

Note: The above is a guide only as to what may be used when decontaminating equipment, spillage areas or situations as directed by AQIS.

Premises holders should also contact their respective State/Territory governments to check what chemicals are approved in their States/Territory.

Chemicals must be applied in accordance with the label directions.

Notes:

Some disinfectants may cause damage to some metals such as aluminium, chrome and similar alloys. The premises holder must be aware that

possible damage to surfaces from any treatments may occur. Disinfectants should be used as per manufacturer's directions and where corrosion

or discolouring is of concern, washed off after use.

Care must be taken when applying the disinfectant to ensure that the surface is not damaged. The first application should be on a small, less

exposed area and should be checked for damage such as paint tainting (colour change), smearing or bubbling before the disinfectant is applied to

the rest of the item.

Any damage to goods from disinfection procedures should be reported to AQIS Canberra Office.

Protective clothing including boots, safety glasses and gloves (preferably disposable) should be worn when handling any chemical disinfectant

used for decontamination. Particular care must be taken to avoid contact with skin or eyes, and inhalation of fumes or powders.

Acid and alkaline based disinfectants must not be mixed.

Note: In times of a disease outbreak emergency, consult with your regional office for alternative sources of information in relation to specific

diseases.

If you require further information, please contact:

AQIS Compliance Agreement Management Unit, Canberra

Phone: 02 6272 3072

Fax: 02 6272 5888

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Attachment 2

Quarantine Pesticide Application Logbook Date Pesticide Treated areas/ Comments Administered by

22

Attachment 3

Quarantine Record

Sample

No

Date of

arrival

Q

Entry

Permit

No

Type of

Goods

Origin Amount Analysis

details

Disposal details including

date, amount and method

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University Access to Parking for AQIS Officers.

Red parking permits should be asked for at Security on arrival on campus. See map below for location of

security and location of E5a car park

Rear car park E5a

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FUNGUS (big autoclave) For WASTE Dispose waste ASAP after autoclaving

TIME NAME OF USER CYCLE TYPE OF LOAD EXT.# DEPARTMENT SUPERVISOR

6:30-8am Elsa Mardones wet Quarantine waste 8233 CBMS Elsa

8-9.30am

9.30-11am

11-12.30am

12:30-2pm

2-3:30pm

3:30-5pm

5-6:30pm

BACTERIUM (new autoclave) NO WASTE TIME NAME OF USER CYCLE TYPE OF LOAD EXT.# DEPARTMENT SUPERVISOR

6:30-8am

8-9:30am

9:30-11am

11-12:30pm

12:30- 2pm

2-3:30pm

3:30-5pm

5-6:30pm

MINI AUTOCLAVE (Don't autoclave AGAR) TIME NAME OF USER CYCLE TYPE OF LOAD EXT.# DEPARTMENT SUPERVISOR

8-9am

9-10am

10-11am

11-12am

12-1pm

1-2pm

2-3pm

3-4pm

4-5pm

Last autoclave user MUST turn autoclave /s and boiler off, at anytime. Emergencies contact for assistance Elsa Mardones x 8233, E8A106