Abstracts / Placenta 35 (2014) A1eA23A22

risk of stillbirth at GWNwas defined as a number of womenwith stillbirthat GW. N divided by number of women giving birth at GW N.Results: A total of 32 (29.4%) women experienced stillbirth at median GWof 31 (range, 24±38). Preterm birth (GW37) occurred in 52 (67.5%) of the 77live born infants. Only 25 (22.9%) women had full-term (GW37) live-borninfants. The prospective risk of stillbirth was 29.4% (32/109), 27.5% (25/91),20.9% (14/67) and 13.0% (6/46) for womenwho reached GW 24, 28, 32, and36, respectively.Conclusions: As womenwith PMD faces at an extraordinary higher risk ofIUFD, early admission to the hospital and intensive monitoring of foetalstatus should be considered, although whether this policy improvesoutcome has not been validated.

O-097.RADIOACTIVE CESIUM AND POTASSIUM LEVELS IN HUMAN PLACENTA:AFTER THE FUKUSHIMA NUCLEAR POWER PLANT ACCIDENT

Makoto Kawamur a,b, Hiroshi Anbe b, Fujimori Keiya a. a Fukushima MedicalUniversity, Department of Obstetrics and Gynecology, Japan; bMinamisomaCity General Hospital, Department of Obstetrics and Gynecology, Japan

Background: The Great East Japan earthquake (followed by large-scaletsunamis) struck Northeastern Pacific Coast of Japan on Mar 11, 2011. Itcaused TEPCO's Fukushima Daiichi Nuclear Power Plant (NPP) accident,accompanied by radioactive pollution in Fukushima prefecture. Minami-soma City General Hospital, just 23km away from the NPP, restarted theObstetric services in April 2012. We examined the placental radioactiveCesium and Potassium levels.

Methods: We measured the radioactive contents of the 40samples ofplacenta after the delivery, using high-purity Germanium detectors forGamma-ray spectrometry (at National Institute of Public Health, Wako,Saitama). All the parturients underwent the Whole-Body Counting (WBC)test of radioactivity.Results: The radioactive content levels are as follows. 134Cs: 0.36Bq/kg,137Cs: 0.58Bq/kg, total-Cs: 0.94Bq/kg, 40K (natural radioactive): 53.1Bq/kg.Radioactive Cs (from the NPP accident) levels were about 1/50 of thenatural radioactive, and much lower than the '87 Italian data, 2years afterthe Chernobyl NPP accident (total-Cs:11.5Bq/kg). All of the parturientswere negative on the WBC test (with cut-off value of 250Bq/body).Conclusion: The Cs levels were much lower than natural radioactive (40K)level, which suggests the efforts to reduce external/internal radioactiveexposure in the area has been successful.

O-102.DENTAL INFECTION OF PORPHYROMONAS GINGIVALIS INDUCESPRETERM BIRTH

Mutsumi Miyauchi a, Hisako Furusho a, Atsuhiro Nagasaki a, SatoshiUrabe b, Haruhisa Konishi b, Hiroshi Miyoshi b, Yoshiki Kudo b, TakashiTakata a. aDepartment of Oral and Maxillofacial Pathobiology, Institute ofBiomedical and Health Sciences, Hiroshima University, Japan; bDepartmentof Obstetrics and Gynecology, Hiroshima University, Japan

Objectives: Epidemiological studies revealed a link between periodontaldisease and preterm delivery, however, the mechanism of the link remainsunclear. The present study was aimed to examine the influence of Por-phyromonas gingivalis (Pg): one of main periodontal pathogens) on pre-term birth with a Pg dental infection mouse model and in vitro analyseswith trophoblasts and related cells Methods: In vivo experiment: 8-wks-old\C57Bl/6J mice were infected with Pg-w83-strain (108 CFU) from thepulp chamber (Pg group). Mating was started at 6-wks post-infectionwhen a periapical granuloma was established. Gestational day (gd) andbirth weight were examined.; At gd15, placental tissues were harvested forIHC staining and PCR. Serum, placental tissue and amniotic fluid werecollected for ELISA of cytokines. Uninfected mice were used as negativecontrol (NC). In vitro experiment: HTR-8 (trophoblast), HuhT1 (endothelialcell) and THP1 (monocyte) cell lines were used to assess the effects of Pgand Pg-LPS.

Results: Pg group showed 2 days of preterm birth (p<0.01) and low birthweight (p<0.01). The amnion-epithelium was damaged in placenta of Pggroup and trophoblasts and endothelial cells became degenerative.Moreover, galectin-3 (Gal-3: an immune regulator) suffused the wholeplacental tissue and dramatically increased in amniotic fluid (P<0.01). Theweaker immuno-expression of CD-31 indicated endothelial damage andimmuno-expression of COX-2 and TNF-alpha increased in placental tissueof Pg group. Pg was detected in placental tissue. Pg-LPS up-regulated theexpression of COX-2, TNF-alpha and Gal-3. Moreover, recombinant Gal-3stimulation further increased expressions of them.Conclusion: Dentally infected Pg reaches placental tissue through bloodcirculation and induces preterm birth via upregulation of COX-2, TNF-aand Gal-3 expression in placental tissue.

O-103.EXPRESSION OF TOLL-LIKE RECEPTORS AND PROINFLAMMATORYCYTOKINES IN HUMAN PLACENTA

Hiroaki Moroi, Tomomi Kotani, Yukio Mano, Hiroyuki Tsuda, SeijiSumigama, Fumitaka Kikkawa. Department of Obstetrics and Gynecology,Nagoya University Graduate School of Medicine, Japan

Objectives: Toll-like receptors (TLRs) are found at many sites in humanbody. They play important roles of the innate immune system. On theother hands, chorioamnionitis (CAM) is thought to be a cause of pretermbirth (PTB) and subsequent neonatal adverse effect. It is well known thatadministration of lipopolysaccharide (LPS) which is recognized by TLR4leads to preterm birth in animal model, and it supports that TLR4 isassociated with CAM and PTB. However, there is little data about othermembers of TLRs. In this study, we focused on other TLR members andundertook investigation of the expression of these in the human pretermplacenta to reveal the connection between TLRs and CAM.

Methods: We extracted the 15 cases of PTB in our hospital. Immunohis-tochemistry was performed with each TLR antigen on microscopic sectionof the each placental tissues. Furthermore, we preformed immno-fluorescence staining with TLR and IL6/8 antigen in the slides which wereobserved strongly positive staining of TLRs. On the other hand, we cor-rected human fetal membranes from the patients who received cesareansection without any perinatal complications. Tissues were cultured andstimulated by each TLR ligands, and the production of proinflamｍatorycytokines are evaluated by ELISA.Results: Immunohistochemistry showed increased expression of TLR4 and5 in placental tissue of CAM (+) samples. But TLR 2 or 6 didn’t show sig-nificant difference between CAM(+) and CAM(-) groups. IL6/8 also stronglyexpressed in the same site inwhich TLR5 was stained. In the tissue culture,IL6 expression was remarkably increased by TLR2/4/5 ligands. But nosignificant difference of IL8 was seen.Conclusions: This study shows that, in chorioamnionitis, TLRs expressionare elevated in the fetal membrane, and it may provide increased proin-flammatory cytokine production. Therefore TLRs may pray important rolesin human chorioamnionitis.

O-104.DNA METHYLATION ANALYSIS OF A CPG-RICH PROMOTER REGION OFC19MC IN HUMAN TROPHOBLAST CELLS

Hironori Takahashi a,b, Akihide Ohkuchi a, Tomoyuki Kuwata a, RieUsui a, Kunio Kikuchi b, Shigeki Matsubara a, Mitsuaki Suzuki a, ToshiyukiTakeshita c, Toshihiro Takizawa b. aDepartment of Obstetrics andGynecology, Jichi Medical University, Tochigi, Japan; bDepartment ofMolecular Medicine and Anatomy, Nippon Medical School, Tokyo, Japan;cDepartment of Reproductive Medicine, Perinatology and GynecologicOncology, Nippon Medical School, Tokyo, Japan

Objective: In the human, miRNAs derived from the miRNA cluster onhuman chromosome 19 (C19MC), a primate-specific miRNA cluster, havebeen demonstrated to exhibit a placenta-specific expression pattern. TheC19MC is an imprinting gene and is exclusively expressed from a paternal