quantitative analysis of antioxidant content of teas

1
TITLE: ABSORPTION OF NONHEME, BUT NOT HEME IRON, IS SUBSTANTIALLY REDUCED WITH HIGH IRON STORES AUTHOR(S): J.R. Hunt, PhD, RD LEARNING OUTCOME: To understand how the absorption of heme and nonheme iron from a high bioavailability, meat-containing diet, are influenced by iron status, or body iron stores. TEXT: Humans absorb heme iron from meat, poultry and fish more efficiently than nonheme iron, and high consumption may increase body iron stores, and possibly oxidative stress. Results from previous studies of heme and nonheme iron bioavailability, measured separately for men and for women, were combined to model the relative absorption of each form of iron in relation to body iron stores. Iron absorption was measured in healthy men (n14) and premenopausal women (n18) consuming controlled, high bioavailability diets. Adjusted for individual energy intake, the diets provided 10-14 oz/d of meat or poultry, refined grains and cereal products, no coffee or tea, and 75 mg ascorbic acid with each meal. By analysis, the diet contained 12.9 mg iron/2500 kcal, with 1.4 mg or 11% as heme iron. Iron absorption was measured by radiolabeling the 2-d menu with 59 FeCl3 and 55 Fe hemoglobin, and measuring whole body and erythrocyte retention after 2 wk. With all variables logarithmically transformed, total iron absorption (0.4 to 4.5 mg/d) and nonheme iron absorption, but not heme iron absorption, were inversely related to serum ferritin (4 to 308 g/L) (R 2 0.66, p0.01). Subjects with the lowest iron stores absorbed iron mostly in the nonheme form, and heme iron accounted for 15-20% of the iron absorbed. With higher iron stores, nonheme iron absorption decreased, with the result that heme iron accounted for half of the iron absorbed by subjects with serum ferritin 150 g/L. Unlike nonheme iron, heme iron absorption is not substantially reduced as iron stores increase. FUNDING DISCLOSURE: Funded by the US Department of Agriculture. TITLE: QUANTITATIVE ANALYSIS OF ANTIOXIDANT CONTENT OF TEAS AUTHOR(S): L.R. Wager, Undergraduate Student, James Madison University; M.H. Renfroe, PhD, Department of Biology, James Madison University; P.B. Brevard, PhD, RD, FADA, Department of Health Sciences, James Madison University; R.E. Lee, PhD, Department of Mathematics and Statistics, James Madison University, Harrisonburg, VA LEARNING OUTCOME: To compare various types of tea and determine the types that contain the highest antioxidant content. TEXT: Tea has long been praised for its high antioxidant content; however, the processing of Camellia sinensis and various plants used to produce teas may not yield equivalent antioxidant content. The purpose of this study was to determine the antioxidant content of the following different types of commercial tea, as purchased by consumers: peppermint tea (Mentha piperita), two black teas, two green teas, two green decaffeinated teas, oolong tea, white tea, and red tea (Aspalathus linearis). Antioxidant activity was measured by its ability to quench the 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical compared to 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid, a vitamin E analog used as the standard. Both hydrophilic and lipophilic concentrations were determined in order to obtain total antioxidant content; however, lipophilic antioxidant content was negligible. Analysis of variance and a Dunnett’s T3 post-hoc pairwise comparison were used to determine differences in antioxidant content among the types of tea. Many statistically significant differences were found among the teas when comparing the teas on a dry weight or fresh weight basis (p 0.05). Peppermint tea contained significantly higher antioxidant concentration than all other teas, the black teas were significantly higher than the green teas, one brand of decaffeinated green tea was significantly higher than the caffeinated variety, whereas another brand was not different. The red and white teas were significantly lower than other teas. Although processing methods and species characteristics vary among teas, which may account for observed variance in antioxidant content, most teas are a good dietary source of antioxidants. FUNDING DISCLOSURE: None TITLE: EFFECTS OF INDUCTION, CONVENTIONAL, AND MICROWAVE COOKING ON SENSORY QUALITIES AND CAROTENOID RETENTION IN SELECTED VEGETABLES AUTHOR(S): J.A. Driskell, PhD, RD; M.N. Nunn, MS, RD; D.W. Giraud, MS; F.L. Hamouz, PhD, RD; Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE LEARNING OUTCOME: To understand the importance of cooking method on the sensory qualities and carotenoid retention in selected vegetables. TEXT: The method by which foods are cooked may influence the nutrient retention and sensory characteristics. Induction cookery is a newer method of cooking that is convenient and energy efficient. The effects of induction boiling, conventional boiling, and microwave steaming on the sensory qualities and carotenoid retention of broccoli, carrots, green beans, and sweet potatoes were investigated. Color, flavor, and texture of these vegetables were evaluated by a 14-member trained sensory panel. Samples were analyzed for carotenoid content by high- performance liquid chromatography. Significantly higher cooking yields were obtained for vegetables induction or conventionally boiled than microwave steamed. A trained panel judged the color scores of three of the vegetables by cooking method as similar. Equivocal results were observed for flavor. Microwave steamed vegetables were judged to be firmer, sometimes significantly, in texture than conventional boiled with induction boiled being in the middle. No differences in retentions of the carotenoids -carotene, -carotene, and lutein/zeaxanthin were observed for vegetables by cooking method, with the exception of -carotene retention in broccoli and sweet potatoes where retentions were higher for those prepared by induction boiling than microwave steaming. Induction boiling appeared to be as good as or better than conventional boiling or microwave steaming for preparation of these selected vegetables as judged by cooking yields, carotenoid retention, and most of the sensory characteristics. Induction cookery offers food service personnel an energy efficient method for preparing vegetables, and likely other foods, with high nutrient retention and desirable sensory qualities. FUNDING DISCLOSURE: Funded by Nebraska Agricultural Research Division. TITLE: FUNCTIONALITY OF SOY MILKS AS A SUBSTITUTE FOR COW’S MILK IN FOOD PRODUCTS: MUFFINS, ICE CREAM, QUICHE, POPOVERS AUTHOR(S): R.B. Swanson, PhD; S.L. Vondekamp, BS; C.N. Knight, BS; T.A. Stryszko, BS; P.J. Milly, MS; J.A. Petrecca, BS; M.E. Kozinsky, BS; C.C. O’Barr, BS; A.M. Camp, BS; R.D. Rooker, BS; H.A. Johnson, BS; Department of Foods and Nutrition, University of Georgia, Athens, GA LEARNING OUTCOME: To compare functional performance of 2 soy milks (1 full-fat, 1 light) to whole-cow’s milk in 4 food products (ice cream, popovers, quiche, muffins) in which the functional roles of milk varied. TEXT: Soy milk is lactose and cholesterol-free; it has a low starch content and is a potential source of isoflavones. Protein content approximates cow’s milk. Full-fat and reduced-in-fat cow and soy milks are available. Popularity of soy milk is increasing. Limited non-anecdotal information on functional performance of soy milk in food products is available; cow milk remains the gold standard. Two commercially available soy milks were substituted for 100% of the cow milk in each product formula; whole cow milk was the control. All non-milk ingredients and preparation procedures within each product were held constant; 2 replications were obtained. Data were analyzed with ANOVA (p0.05) and SNK, where appropriate. All milks differed significantly from each other in total solids, pH, viscosity, particle size distribution and color (L*, a*, b*), suggesting potential effects with substitution. In muffins, texture profile analysis (50- kg-TA.XT2, crossarm speed 5mm/sec, 50% compression) revealed no differences in hardness, cohesiveness, gumminess or chewiness. Neither popover volume nor shape uniformity (AACC Method 10-90) differed significantly. Neither the muffin nor popover exhibited surface color (L*, a*, b*) differences (Minolta Spectrophotometer CM-508d, white standard calibration cap). Penetration of the quiche surface with a 2 mm probe (50- kg-TA.XT2, crossarm speed 2 mm/sec, 5 mm depth) revealed no differences in firmness. In custard-based ice cream, percentage overrun, indicative of air incorporation, differed significantly, with both soy milks (% overrun 30.4 6.4 and 28.82.6) exceeding the percentage found with cow milk (14.85.6%). Preliminary sensory evaluation supports these non-sensory results and suggests that flavor will determine consumer acceptability. FUNDING DISCLOSURE: none MONDAY, SEPTEMBER 18 POSTER SESSION: SCIENCE/EDUCATION/MANAGEMENT/FOODSERVICE/CULINARY/RESEARCH A-42 / August 2006 Suppl 2—Abstracts Volume 106 Number 8

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Page 1: Quantitative Analysis of Antioxidant Content of Teas

TITLE: ABSORPTION OF NONHEME, BUT NOT HEME IRON, ISSUBSTANTIALLY REDUCED WITH HIGH IRON STORES

AUTHOR(S): J.R. Hunt, PhD, RD

LEARNING OUTCOME: To understand how the absorption ofheme and nonheme iron from a high bioavailability, meat-containingdiet, are influenced by iron status, or body iron stores.

TEXT: Humans absorb heme iron from meat, poultry and fish moreefficiently than nonheme iron, and high consumption may increasebody iron stores, and possibly oxidative stress. Results from previousstudies of heme and nonheme iron bioavailability, measuredseparately for men and for women, were combined to model therelative absorption of each form of iron in relation to body iron stores.Iron absorption was measured in healthy men (n�14) andpremenopausal women (n�18) consuming controlled, highbioavailability diets. Adjusted for individual energy intake, the dietsprovided 10-14 oz/d of meat or poultry, refined grains and cerealproducts, no coffee or tea, and �75 mg ascorbic acid with each meal.By analysis, the diet contained 12.9 mg iron/2500 kcal, with 1.4 mgor 11% as heme iron. Iron absorption was measured by radiolabelingthe 2-d menu with 59FeCl3 and 55Fe hemoglobin, and measuringwhole body and erythrocyte retention after 2 wk. With all variableslogarithmically transformed, total iron absorption (0.4 to 4.5 mg/d)and nonheme iron absorption, but not heme iron absorption, wereinversely related to serum ferritin (4 to 308 �g/L) (R2 � 0.66,p�0.01). Subjects with the lowest iron stores absorbed iron mostly inthe nonheme form, and heme iron accounted for 15-20% of the ironabsorbed. With higher iron stores, nonheme iron absorptiondecreased, with the result that heme iron accounted for half of theiron absorbed by subjects with serum ferritin �150 �g/L. Unlikenonheme iron, heme iron absorption is not substantially reduced asiron stores increase.

FUNDING DISCLOSURE: Funded by the US Department ofAgriculture.

TITLE: QUANTITATIVE ANALYSIS OF ANTIOXIDANTCONTENT OF TEAS

AUTHOR(S): L.R. Wager, Undergraduate Student, James MadisonUniversity; M.H. Renfroe, PhD, Department of Biology, JamesMadison University; P.B. Brevard, PhD, RD, FADA, Department ofHealth Sciences, James Madison University; R.E. Lee, PhD,Department of Mathematics and Statistics, James MadisonUniversity, Harrisonburg, VA

LEARNING OUTCOME: To compare various types of tea anddetermine the types that contain the highest antioxidant content.

TEXT: Tea has long been praised for its high antioxidant content;however, the processing of Camellia sinensis and various plants used toproduce teas may not yield equivalent antioxidant content. The purposeof this study was to determine the antioxidant content of the followingdifferent types of commercial tea, as purchased by consumers:peppermint tea (Mentha piperita), two black teas, two green teas, twogreen decaffeinated teas, oolong tea, white tea, and red tea (Aspalathuslinearis). Antioxidant activity was measured by its ability to quench the2,2�-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radicalcompared to 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid, avitamin E analog used as the standard. Both hydrophilic and lipophilicconcentrations were determined in order to obtain total antioxidantcontent; however, lipophilic antioxidant content was negligible. Analysisof variance and a Dunnett’s T3 post-hoc pairwise comparison were usedto determine differences in antioxidant content among the types of tea.Many statistically significant differences were found among the teaswhen comparing the teas on a dry weight or fresh weight basis (p �0.05). Peppermint tea contained significantly higher antioxidantconcentration than all other teas, the black teas were significantlyhigher than the green teas, one brand of decaffeinated green tea wassignificantly higher than the caffeinated variety, whereas another brandwas not different. The red and white teas were significantly lower thanother teas. Although processing methods and species characteristicsvary among teas, which may account for observed variance inantioxidant content, most teas are a good dietary source of antioxidants.

FUNDING DISCLOSURE: None

TITLE: EFFECTS OF INDUCTION, CONVENTIONAL, ANDMICROWAVE COOKING ON SENSORY QUALITIES ANDCAROTENOID RETENTION IN SELECTED VEGETABLES

AUTHOR(S): J.A. Driskell, PhD, RD; M.N. Nunn, MS, RD;D.W. Giraud, MS; F.L. Hamouz, PhD, RD; Department of Nutritionand Health Sciences, University of Nebraska, Lincoln, NE

LEARNING OUTCOME: To understand the importance of cookingmethod on the sensory qualities and carotenoid retention in selectedvegetables.

TEXT: The method by which foods are cooked may influence thenutrient retention and sensory characteristics. Induction cookery is anewer method of cooking that is convenient and energy efficient. Theeffects of induction boiling, conventional boiling, and microwavesteaming on the sensory qualities and carotenoid retention of broccoli,carrots, green beans, and sweet potatoes were investigated. Color, flavor,and texture of these vegetables were evaluated by a 14-member trainedsensory panel. Samples were analyzed for carotenoid content by high-performance liquid chromatography. Significantly higher cooking yieldswere obtained for vegetables induction or conventionally boiled thanmicrowave steamed. A trained panel judged the color scores of three ofthe vegetables by cooking method as similar. Equivocal results wereobserved for flavor. Microwave steamed vegetables were judged to befirmer, sometimes significantly, in texture than conventional boiled withinduction boiled being in the middle. No differences in retentions of thecarotenoids �-carotene, �-carotene, and lutein/zeaxanthin were observedfor vegetables by cooking method, with the exception of �-caroteneretention in broccoli and sweet potatoes where retentions were higherfor those prepared by induction boiling than microwave steaming.Induction boiling appeared to be as good as or better than conventionalboiling or microwave steaming for preparation of these selectedvegetables as judged by cooking yields, carotenoid retention, and most ofthe sensory characteristics. Induction cookery offers food servicepersonnel an energy efficient method for preparing vegetables, andlikely other foods, with high nutrient retention and desirable sensoryqualities.

FUNDING DISCLOSURE: Funded by Nebraska AgriculturalResearch Division.

TITLE: FUNCTIONALITY OF SOY MILKS AS A SUBSTITUTE FORCOW’S MILK IN FOOD PRODUCTS: MUFFINS, ICE CREAM, QUICHE,POPOVERS

AUTHOR(S): R.B. Swanson, PhD; S.L. Vondekamp, BS; C.N. Knight, BS;T.A. Stryszko, BS; P.J. Milly, MS; J.A. Petrecca, BS; M.E. Kozinsky, BS;C.C. O’Barr, BS; A.M. Camp, BS; R.D. Rooker, BS; H.A. Johnson, BS;Department of Foods and Nutrition, University of Georgia, Athens, GA

LEARNING OUTCOME: To compare functional performance of 2 soymilks (1 full-fat, 1 light) to whole-cow’s milk in 4 food products (ice cream,popovers, quiche, muffins) in which the functional roles of milk varied.

TEXT: Soy milk is lactose and cholesterol-free; it has a low starch contentand is a potential source of isoflavones. Protein content approximatescow’s milk. Full-fat and reduced-in-fat cow and soy milks are available.Popularity of soy milk is increasing. Limited non-anecdotal information onfunctional performance of soy milk in food products is available; cow milkremains the gold standard. Two commercially available soy milks weresubstituted for 100% of the cow milk in each product formula; whole cowmilk was the control. All non-milk ingredients and preparation procedureswithin each product were held constant; 2 replications were obtained.Data were analyzed with ANOVA (p�0.05) and SNK, where appropriate.All milks differed significantly from each other in total solids, pH,viscosity, particle size distribution and color (L*, a*, b*), suggestingpotential effects with substitution. In muffins, texture profile analysis (50-kg-TA.XT2, crossarm speed 5mm/sec, 50% compression) revealed nodifferences in hardness, cohesiveness, gumminess or chewiness. Neitherpopover volume nor shape uniformity (AACC Method 10-90) differedsignificantly. Neither the muffin nor popover exhibited surface color (L*,a*, b*) differences (Minolta Spectrophotometer CM-508d, white standardcalibration cap). Penetration of the quiche surface with a 2 mm probe (50-kg-TA.XT2, crossarm speed 2 mm/sec, 5 mm depth) revealed no differencesin firmness. In custard-based ice cream, percentage overrun, indicative ofair incorporation, differed significantly, with both soy milks (% overrun�30.4� 6.4 and 28.8�2.6) exceeding the percentage found with cow milk(14.8�5.6%). Preliminary sensory evaluation supports these non-sensoryresults and suggests that flavor will determine consumer acceptability.

FUNDING DISCLOSURE: none

MONDAY, SEPTEMBER 18

POSTER SESSION: SCIENCE/EDUCATION/MANAGEMENT/FOODSERVICE/CULINARY/RESEARCH

A-42 / August 2006 Suppl 2—Abstracts Volume 106 Number 8