QoD (HB) 5-31-11QoD (HB) 5-31-11

Do you remember what a plasmid Do you remember what a plasmid is?is?

Plan of the Day (HB)Plan of the Day (HB)How engineering bacteria works Overview and points to consider for

pGLO bacterial transformation lab tomorrow

PresentationsPresentations

How can bacteria exchange genes?

Through tunnel like connectionsViruses carry bacterial genesBacteria take up DNA from

surrounding environment

Recombinant DNA Technology

• Some Bacteria are used to mass produce specific desirable genes and proteins

Cutting and Pasting DNA

• Restriction enzymes cut DNA molecules at specific locations

• Fragments of DNA spliced together from two different sources produces a recombinant DNA molecule

Cloning of a human gene for a hypothetical protein V

Applications of recombinant DNA technology

• Bacteria engineered to clean up toxic waste• Mass-production of useful chemicals• Mass production of proteins and hormones for

medical uses• Develop vaccines against disease-causing

microbes• Develop genetically modified multicellular

organisms

pGLO bacterial transformation lab preparation

http://biologywithsaxe.blogspot.com/2009/01/lab-9-bacterial-transformation-using.html

Allows bacteria to survive in the presence of ampicillin

In the presence of arabinose produces a protein that will turn on the GFP gene

When turned on will produce a fluorescent green protein that will glow under UV light