Puccinia triticina Puccinia triticina virulencevirulence

phenotypes phenotypes present in Uruguay present in Uruguay

during 2004-2010during 2004-2010

Silvia GermánSilvia Germán

La EstanzuelaLa Estanzuela

2011 North American Rust Workers Meeting2011 North American Rust Workers MeetingSt. Paul, Minnesta, USASt. Paul, Minnesta, USA

June 12, 2011 June 12, 2011

Wheat leaf rustWheat leaf rust((Puccinia triticinaPuccinia triticina))

SamplesSamples• Trap nurseries Trap nurseries • ExperimentsExperiments• CropsCrops• 1 – 2 single pustule isolates per sample 1 – 2 single pustule isolates per sample

Differential setDifferential setLr 1 2a 2c 3Lr 1 2a 2c 3Lr 9 16 24 26Lr 9 16 24 26Lr 3ka 11 17 30Lr 3ka 11 17 30Lr 10 20 .....Lr 10 20 .....

NomenclatureNomenclatureLong and Kolmer, 1989Long and Kolmer, 19893 letter3 letter Prt Prt code + code +Virulence to Virulence to Lr10 andLr10 and Lr20Lr20 MDTMDT

MCR-10MCR-10 MFP-20MFP-20

TDD-10,20TDD-10,20

METHODOLOGYMETHODOLOGY

Race 2004 2005 2006 2007 2008 2009 2010 Total

Total Ner of isolates 100 175 134 122 124 81 134 870

No of races 19 30 24 28 20 16 12 69

No of races freq > 5% 5 5 4 3 6 6 8 15

No races freq >10% 3 3 3 3 5 4 4 12

Number of Number of P. triticinaP. triticina isolates studied, tolal numer of races isolates studied, tolal numer of racesand races present with more than 5 and 10% frequencyand races present with more than 5 and 10% frequency

in Uruguay. 2004-2010.in Uruguay. 2004-2010.

Avirulence/virulence formulae of more frequent races, Avirulence/virulence formulae of more frequent races, and year of first detectionand year of first detection

First

detection

MCD-10 2a,2c,3ka,9,11,16,20,24,30 / 1,3,10,17,26 2001

MCD-10,20 2a,2c,3ka,9,11,16,24,30 / 1,3,10,17,20,26 1999

MCP-10 2a,2c,9,11,16,20,24 / 1,3,3ka,10,17,26,30 2000

MDP 2a,2c,9,10,11,16,20,26 / 1,3,3ka,17,24,30 2002

MFP 2a,2c,9,10,11,16,20 / 1,3,3ka,17,24,26,30 2001

MDP-10,20 2a,2c,9,11,16,26 / 1,3,3ka,10,17,20,24,30 2004

MFP-10,20 2a,2c,9,11,16 / 1,3,3ka,10,17,20,24,26,30 2004

MDP-20 2a,2c,9,10,11,16,26 / 1,3,3ka,17,20,24,30 2004

MFP-20 2a,2c,9,10,11,16 / 1,3,3ka,17,20,24,26,30 2005

MDR-10,20 2a,2c,9,16,17,26 / 1,3,3ka,10,11,20,24,30 2003

MDT 2a,2c,9,10,16,20,26 / 1,3,3ka,11,17,24,30 2010

MDT-10,20 2a,2c,9,16,26 / 1,3,3ka,10,11,17,20,24,30 2007

MFT-10,20 2a,2c,9,16 / 1,3,3ka,10,11,17,20,24,26,30 2007

TDT-10,20 9,16,26 / 1,2a,2c,3,3ka,10,11,17,20,24,30 2010TFT-10,20 9,16 / 1,2a,2c,3,3ka,10,11,17,20,24,26,30 2010

Race Avirulence / Virulence formula

Frequency of important Frequency of important Puccinia triticinaPuccinia triticina isolates isolatesand affected cultivares Uruguay. 2004-2010.and affected cultivares Uruguay. 2004-2010.

First

detection 2004 2005 2006 2007 2008 2009 2010 Epidemic on

MCD-10 2001 15.0 8.0

MCD-10,20 1999 6.0 2.3 0.7 3.3 I Mirlo

MCP-10 2000 28.0 15.4 1.5 1.2 K.Don Enrique

MDR-10,20 2003 17.0 32.0 20.1 3.3 1.6I.Churrinche I.Torcaza

MDP 2002 4.0 3.4 0.7 4.1 7.3 13.6 10.4

MFP 2001 6.0 0.6 1.6 4.0 21.0 30.6

MDP-10,20 2004 4.0 10.9 18.7 16.4 11.3 3.7 5.2

MFP-10,20 2004 1.0 2.2 1.6 16.9 18.5 6.7

MDP-20 2004 1.0 5.1 6.0 0.8 2.4 3.7 6.7

MFP-20 2005 1.7 28.4 4.1 12.9 13.6 1.5 I Tero

MDT-10,20 2007 23.8 17.7 8.6 2.2

MFT-10,20 2007 13.9 11.3 6.2 0.7

TDT-10,20 2010 14.9 Atlax

TFT-10,20 2010 11.9 Atlax

MDT 2010 7.5

Race

Frequency

Virulence formulae and year of first detection of other Virulence formulae and year of first detection of other Puccinia triticinaPuccinia triticina races present in Uruguay races present in Uruguay

during 2004-2010.during 2004-2010.

First First

Race Virulence formula detection Race Virulence formula detection

CCT 3,3ka,11,17,23,26,30 1997 MFD-10,20 1,3,10,17,20,24,26 2002

CGT 3,3ka,11,16,17,23,30 1991 MFR-10,20 1,3,3ka,10,11,20,24,26,30 2004

CHT 3,3ka,11,16,17,23,26,30 1997 MFT 1,3,3ka,11,17,24,26,30 2009

MBD-10,20 1,3,17,10,20 1998 MFT-20 1,3,3ka,11,17,20,24,26,30 2008

MBP-10,20 1,3,3ka,10,17,20,30 2006 MHP-10 1,3,3ka,10,16,17,26,30 2002

MCB-10 1,3,10,26 2004 MMD-10 1,3,9,10,17,26 2006

MCG-10 1,3,10,11,26 1989 MMD-10,20 1,3,9,10,17,20,26 2006

MCP-10,19 1,3,3ka,10,17,19,26,30 2006 MRD-10 1,3,9,10,16,17,26 2008

MCP-10,20 1,3,3ka,10,17,20,26,30 2002 SNG-10 1,2a,2c,9,11,24 2003

MCR-10 1,3,3ka,10,11,26,30 1989 SPG-10 1,2a,2c,9,11,20,24,26 2002

MCT-10 1,3,3ka,10,11,17,26,30 1992 TDD-10,20 1,2a,2c,3,10,17,20,24 1995

MDD-10,20 1,3,10,17,20,24 2002 TDG-10,20 1,2a,2c,3,10,11,20,24 1999

MDP-10 1,3,3ka,10,17,24,30 2006 TDP-10,20 1,2a,2c,3,3ka,10,17,20,24,30 2007

MDR-10 1,3,3ka,10,11,24,30 2005 TFP 1,2a,2c,3,3ka,17,24,26,30 2007

MDR-20 1,3,3ka,11,20,24,30 2002 TPR-20 1,2a,2c,3,3ka,9,11,20,24,26,30 2008

Year 1 2a 2c 3 9 16 24 26 3ka 11 17 30 10 20 19

2004 99.0 3.0 3.0 100.0 0.0 0.0 43.0 68.0 71.0 27.0 72.0 71.0 88.0 41.0 0.0

2005 98.9 3.4 3.4 100.0 1.7 0.0 65.1 39.4 81.1 46.3 57.7 81.1 86.3 65.1 0.0

2006 94.8 1.5 1.5 99.3 3.0 0.7 85.1 50.0 94.8 32.8 73.9 94.8 59.7 85.1 4.5

2007 100.0 12.3 12.3 93.4 9.8 0.0 88.5 42.6 86.9 55.7 83.6 86.9 82.0 82.0 1.6

2008 100.0 5.6 5.6 100.0 7.3 4.0 93.5 56.5 95.2 36.3 92.7 95.2 68.5 82.3 0.0

2009 97.5 3.7 3.7 100.0 3.7 3.7 93.8 67.9 98.8 21.0 97.5 98.8 42.0 59.3 0.0

2010 100.0 26.9 26.9 100.0 0.0 0.0 100.0 53.0 100.0 38.8 100.0 100.0 41.8 50.0 0.0

B 0-20% BI 20-40% I 40-60% IA 60-80% A 80-100%

% virulence% virulenceLr3bg Lr3bg high (2006 +)high (2006 +)Lr14a Lr14a 100100Lr14b Lr14b high highLr21 Lr21 00Lr23Lr23 int-highint-highLr25 Lr25 0 (2006 +, mixture)0 (2006 +, mixture)Lr39/41 Lr39/41 2009 need confirm2009 need confirmLr42 Lr42 2009 need confirm2009 need confirmLr47 Lr47 0 (2009 +)0 (2009 +)

APR genesAPR genesLr12Lr12 inefectiveinefectiveLr13Lr13 ineffectiveineffectiveLr22aLr22a effectiveeffectiveLr22b Lr22b ineffectiveineffectiveLr35 Lr35 effectiveeffectiveLr37Lr37 ineffectiveineffectiveLr34Lr34 50-60 M 50-60 MLr46Lr46 70-80 MSS 70-80 MSS

Virulence frequency to Virulence frequency to LrLr genes. 2004-2010. genes. 2004-2010.

Field effectiveField effectiveLr29 Lr29 00Lr32 Lr32 40 MRMS40 MRMSLr33 Lr33 60 MRMS60 MRMSLr36 Lr36 00Lr45 Lr45 00Lr47 Lr47 00Lr51 Lr51 00

Foto grupo de trabajoFoto grupo de trabajo

RichardRichardGarcíaGarcía

NoeliaNoeliaPerezPerez

FernandoFernandoPereiraPereira

Thank youThank youGraciasGracias