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PERFECT SCORE BIOLOGY 2011 1 BAHAGIAN SEKOLAH BERASRAMA PENUH DAN SEKOLAH KECEMERLANGAN KEMENTERIAN PELAJARAN MALAYSIA PERFECT SCORE BIOLOGY 2011 Student’s Module PAPER 3 QUESTION 1

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  • PERFECT SCORE BIOLOGY 2011

    1

    BAHAGIAN SEKOLAH BERASRAMA PENUH DAN

    SEKOLAH KECEMERLANGAN

    KEMENTERIAN PELAJARAN MALAYSIA

    PERFECT SCORE BIOLOGY

    2011 Students Module

    PAPER 3

    QUESTION 1

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    Question 1 :

    No. Questions Marks Student notes

    1 A group of students carried out an experiment to study the effect of the concentration of

    nutrients on the activity of yeast . Diagram 1.1 shows the method used by the students.

    The initial height of the coloured liquid in the manometer is shown in Diagram 1.2.

    The experiment was repeated using different concentrations of glucose. Table 1.1 shows the

    results of the experiment after 10 minutes.

    Diagram 1.1

    DIAGRAM 1.2

    rubber tubing

    Manometer with

    coloured liquid

    Initial height of

    coloured liquid

    Boiling tube containing

    yeast suspension

    Glass tube

    clip

    Rubber stopper

    Initial height of coloured liquid :

    cm

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    Percentage concentration of glucose / %

    Final height of coloured liquid in the manometer after 10 minutes /cm

    10

    15

    20

    ..

    ..

    ..

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    No. Questions Marks Student notes

    (a) Complete Table 1.2 by recording the height of coloured liquid in the manometer after 10 minutes

    (b) (i) Based on Table 1.1, state two observations . Observation 1 :

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    Observation 2 :

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    (ii) State the inference which corresponds to the observation in 1(b(i). Inference 1 :

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................ Inference 2 :

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    3

    3

    3

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    (c) Complete Table 1.2 for the three variables based on the experiment.

    Variable Method to handle the variable

    Manipulated variable: .............................................

    .............................................

    .............................................

    ....................................................

    ....................................................

    ....................................................

    Responding variable: .............................................

    .............................................

    .............................................

    ....................................................

    ....................................................

    ....................................................

    Controlled variable : .............................................

    .............................................

    .............................................

    ....................................................

    ....................................................

    ....................................................

    (d) State the hypothesis for the experiment.

    .................................................................................................

    .................................................................................................

    .................................................................................................

    .................................................................................................

    (e) (i) Based on Table 1.1, construct a table and record the results of the experiment which includes the following aspects:

    Percentage concentration of glucose

    Height of coloured liquid

    The rate of the activity of yeast

    3

    3

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    (e) (ii) Draw a graph of the rate of the activity of yeast against the concentration of glucose

    (iii) Based on the graph in 1(e)(ii), state the relationship between

    the rate of the activity of yeast and the concentration of

    glucose. Explain your answer.

    .................................................................................................

    .................................................................................................

    .................................................................................................

    .................................................................................................

    .................................................................................................

    (f) Based on the experiment, define anaerobic respiration in yeast operationally.

    .................................................................................................

    .................................................................................................

    .................................................................................................

    .................................................................................................

    .................................................................................................

    3

    3

    3

    3

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    (g) The experiment is repeated by using 1 ml of 0.1 mol dm-3 of

    sodium hydroxide solution is added into the boiling tube.

    Predict the manometer reading after 10 minutes. Explain

    your prediction.

    .................................................................................................

    .................................................................................................

    .................................................................................................

    .................................................................................................

    .................................................................................................

    (h) The following list is part of the apparatus and material used in this experiment. Complete Table 1.3 by matching each variable with the apparatus and material used in the experiment.

    Variables Apparatus Material

    Manipulated

    ..............................

    ................................

    Responding

    ...............................

    ...............................

    Controlled

    ...............................

    ...............................

    Table 1.3

    Yeast, metre rule, coloured liquid, electronic balance, glucose solution, measuring cylinder

    3

    3

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    Question 2 :

    No. Questions Marks Student notes

    2 Lemna minor is a species of free-floating aquatic plants from the duckweed family Lemnaceae. The plants grow mainly by vegetative reproduction: two daughter plants bud off from the adult plant. An experiment is carried out to investigate the effect of abiotic factor such as pH on Lemna sp. growth. Experiment is done under controlled conditions: 12 hours a day light exposure and using the same Knops solution. Petri dish is filled with 20 ml Knops solution with different pH value and 5 Lemna sp. each. The Knops solution is treated by adding acid or alkali to achieve the pH value needed. ** Knops solution is a solution which contains essential nutrient for plants growth.

    Figure 1 After 7 days, the observation is made and the result shown in Table 1.1 .

    pH value

    Petri dish

    Number of Lemna sp.

    2

    Lemna minor

    Petri dish

    Knops solution

  • PERFECT SCORE BIOLOGY 2011

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    3

    5

    7

    9

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    11

    Table 1.1

    No. Questions Marks Student notes

    (a) State the number of Lemna sp. in the spaces provided in Table 1.1

    (b) (i) Based on Table 1, state two different observations .

    Observation 1 :

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    Observation 2 :

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    (ii) State the inferences which corresponds to the observations in 1(b)(i). Inference 1 :

    ................................................................................................

    ................................................................................................

    ................................................................................................

    3

    3

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    ................................................................................................ Inference 2 :

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    (c) Complete Table 1.4 to show the variables involved in the experiment and how the variables are operated.

    Variable Method to handle the variable

    Manipulated variable: .............................................

    .............................................

    .............................................

    ....................................................

    ....................................................

    ....................................................

    Responding variable: .............................................

    .............................................

    .............................................

    ....................................................

    ....................................................

    ....................................................

    Controlled variable : .............................................

    .............................................

    .............................................

    ....................................................

    ....................................................

    ....................................................

    3

    3

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    (d) State the hypothesis for this experiment.

    .................................................................................................

    .................................................................................................

    .................................................................................................

    .................................................................................................

    (e) (i) Construct a table and record the results of the experiment. Your table should contain the following title.

    pH of water

    Number of Lemna sp.

    (e) (ii) Plot a graph showing the number of Lemna sp against the pH in the graph below

    (iii) Referring to the graph in (e) (ii), describe the relationship

    between the Lemna sp growth and the condition of the

    medium.

    .................................................................................................

    .................................................................................................

    .................................................................................................

    .................................................................................................

    3

    3

    3

    3

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    (f) Based on the experiment, define operationally the abiotic factor in an ecosystem. .................................................................................................

    .................................................................................................

    .................................................................................................

    .................................................................................................

    (g) The effluent from laundry shop flows into a pond nearby, predict the population of Lemna sp in the pond. Explain your answer.

    .................................................................................................

    .................................................................................................

    .................................................................................................

    .................................................................................................

    (h) Classify the biotic and abiotic factors from the list provided below.

    3

    3

    3

    Humidity, light intensity, decomposer,

    parasites, symbiotic organism, soil

    texture, invertebrates, topography

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    Question 3:

    No. Questions Marks Student notes

    1.

    A group of students conducted an experiment to study the effect of light intensity on the population distribution of Lichen on the tree trunk. He placed a 10 cm x 10 cm transparent quadrat on the East-facing surface of the tree trunk. He counted the number of squares that contained half or more than half of the areas covered by the Lichen. Square with less than half of the covered areas were not included. The procedures were repeated for the surfaces that face the direction of North (N), south (S) and west (W). Figure 1 shows how a quadrat is placed on the tree trunk. Each small square represent 1 cm2.

    Figure 1

    Table 1 shows the areas covered by the Lichen on the different surface of the tree trunk.

    Direction/position of surface

    Total surface area covered by Lichen

    East

    60 cm2

  • PERFECT SCORE BIOLOGY 2011

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    South

    35 cm2

    North

    45 cm2

    West

    52 cm2

    Table 1

    10 cm

    10 cm

    10 cm

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    a) Count the total surface area of Lichen for each quadrat and record the answer in the spaces provided in Table 1.

    b) (i) State two different observation based on the diagram in Table 1. Observation 1 :

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    Observation 2 :

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    (ii) State the inferences from the observation in 1 (b) (i).

    Inference 1 :

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................ Inference 2 :

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    3

    3

    3

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    (c) Complete Table 2 based on this experiment.

    Variable Method to handle the variable

    Manipulated variable: .............................................

    .............................................

    .............................................

    ....................................................

    ....................................................

    ....................................................

    Responding variable: .............................................

    .............................................

    .............................................

    ....................................................

    ....................................................

    ....................................................

    Controlled variable : .............................................

    .............................................

    .............................................

    ....................................................

    ....................................................

    ....................................................

    Table 2

    d) State the hypothesis for this experiment:

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    e) (i) Construct a table and record all data collected in this experiment. Your table should have the following aspect:

    Title with correct unit

    Position of direction

    Total surface area covered by Lichen

    3

    3

  • PERFECT SCORE BIOLOGY 2011

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    (ii) Use the graph paper provided to answer this question.

    Using the data in 1 (e) (i), draw a bar chart graph to show the relationship between the population of Lichen against the directions facing on the tree.

    The population of Lichen is represented by the total surface area covered in the quadrat.

    (f) Based on the graph in 1 (e)(ii), explain the relationship between the population distribution of Lichen and the light intensity. ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    (g) State the operational definition for population distribution of Lichen. ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    (h) Lightning strike the tree and cause the tree to fall. The Lichen under study is then exposed to direct sunlight from 7.00 a.m. to 6.00 p.m. daily. Predict what will happen to the total surface area covered by Lichen after a month. Explain your prediction.

    3

    3

    3

    3

  • PERFECT SCORE BIOLOGY 2011

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    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    (i) The following is a list of biotic and abiotic factors. Classify these factors in the Table 3.

    Abiotic factors Biotic factors

    Table 3

    pH of water, pigeon orchid, humidity, bird, temperature, Elodea sp.

    3

    3

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    Question 4 :

    No. Questions Marks Student notes

    4 An experiment was carried out to investigate the water pollution level or BOD in three different locations from a suspected polluted Rivers. Three water samples are collected from these three locations and labelled as P, Q and R as in Diagram 1.

    200 ml of each sample is put in a reagent bottle and added with 1 ml of 0.1% methylene blue solution. All the bottles are kept in dark cupboard.

    Observations are made every minute to see the changes in the methylene blue colour.

    Diagram 1 Table 1 shows the results of this experiment.

    Water sample P Q R

    Time taken for

    methylene

    blue solution

    become

    colourless

    Table 1

    Sample P

    Each sample is added with methylene blue

    solution

    Sample Q Sample R

  • PERFECT SCORE BIOLOGY 2011

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    No. Questions Marks Student notes

    (a) Record the time taken for methylene blue solution become

    colourless in the boxes provided in Table 1.

    (b) (i) Based on Table 1, state two different observations .

    Observation 1 :

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    Observation 2 :

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    (ii) State the inferences which corresponds to the observations in 1(b)(i). Inference 1 :

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    Inference 2 :

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    3

    3

    3

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    (c) Complete Table 2 based on this experiment.

    Variable Method to handle the variable

    Manipulated variable: .............................................

    .............................................

    .............................................

    ....................................................

    ....................................................

    ....................................................

    Responding variable: .............................................

    .............................................

    .............................................

    ....................................................

    ....................................................

    ....................................................

    Controlled variable : .............................................

    .............................................

    .............................................

    ....................................................

    ....................................................

    ....................................................

    Table 2

    (d) State the hypothesis for this experiment.

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    (e) (i) Construct a table and record all the data collected in this

    experiment based on the following criteria:

    Water sample

    Time taken

    3

    3

  • PERFECT SCORE BIOLOGY 2011

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    (f) Based on the data in 1(e) draw a bar chart of time taken for methylene blue solution become colourless against water samples.

    (g) What is the relationship between time taken, oxygen concentration and BOD value of water in this experiment? ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    (h) Based on the result of this experiment, state the operational definition for BOD. ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    (i) This experiment is repeated by using water sample from chicken farm areas. Predict the time taken for methelyne blue to become colourless.

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    3

    3

    3

    3

    3

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    (j) Arrange the water samples from the most polluted to the

    least polluted.

    3

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    Question 5 :

    No. Questions Marks Student notes

    5 Transpiration is the evaporation of water from a plant to the surroundings. The rate of

    transpiration is affected by environmental factors such as temperature.

    A group of students carried out an experiment to study the effect of temperature on the

    rate of transpiration. Diagram 1 shows the set up of the apparatus. An air bubble was

    trapped in the capillary tube. The apparatus was placed in an air-conditioned room at 20oC.

    The time taken for the air bubble to move a distance of 10 cm was recorded. The

    experiment was repeated for a second time to get average readings.

    The experiment is repeated by placing the apparatus at three more different temperatures:

    an air-conditioned room at 25oC , an air-conditioned room at 30oC and in a non air-

    conditioned room at 35oC.

    Table 1 shows the reading of stopwatch for air bubble to move a distance of 10 cm at different temperature

    Diagram 1

  • PERFECT SCORE BIOLOGY 2011

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    Temperature oC

    Time taken for air bubble to move a distance of 10 cm (min)

    First reading

    Second reading Average Reading

    20

    25

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    Temperature Suhu oC

    Time taken for air bubble to move a distance of 10 cm (min)

    First reading

    Second reading

    Average Reading

    30

    35

    No. Questions Marks Student notes

    (a) Record the time taken for the air bubbles to move a

    distance of 10 cm and average reading in Table 1.

    (b) (i) Based on Table 1, state two different observations .

    Observation 1 :

    ................................................................................................

    ................................................................................................

    ................................................................................................

    3

  • PERFECT SCORE BIOLOGY 2011

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    ................................................................................................

    Observation 2 :

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    (ii) State the inferences which corresponds to the observations

    in 1(b)(i).

    Inference 1 :

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    Inference 2 :

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    3

    3

  • PERFECT SCORE BIOLOGY 2011

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    (c) Complete Table 2 based on this experiment.

    Variable Method to handle the variable

    Manipulated variable: .............................................

    .............................................

    .............................................

    ....................................................

    ....................................................

    ....................................................

    Responding variable: .............................................

    .............................................

    .............................................

    ....................................................

    ....................................................

    ....................................................

    Controlled variable : .............................................

    .............................................

    .............................................

    ....................................................

    ....................................................

    ....................................................

    Table 2

    (d) State the hypothesis for this experiment.

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    3

    3

  • PERFECT SCORE BIOLOGY 2011

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    (e) (i) Construct a table and record all the data collected in this

    experiment.

    Your table should have the following aspects:

    - Temperature

    - Average time taken for air bubbles to move a

    distance of 10 cm .

    - Rate of transpiration

    Rate of transpiration = Distance

    Time

    (e) (ii) Using the data in 1(e)(i), draw the graph of the rate of

    transpiration against the temperature

    (f) Based on the graph in 1(e)(ii), explain the relationship

    between the rate of transpiration and temperature.

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    (g) Based on the result of this experiment, state the operational

    definition for process of transpiration.

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    3

    3

    3

    3

  • PERFECT SCORE BIOLOGY 2011

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    (h) If the surface of the leaves of a plant at temperature of 35

    oC are covered with vaselin, predict the time taken for air

    bubble to move a distance of 10 cm. Explain your

    prediction.

    ................................................................................................

    ................................................................................................

    ................................................................................................

    ................................................................................................

    (i) The following list is a factor that affecting transpiration.

    Classify the factors into two group in Table 3.

    Environmental factor Morphology factors

    Table 3

    Relative humidity Kelembapan relatif

    cuticle kutikel

    air movement pergerakan angin

    stomata stomata

    light intensity keamatan cahaya

    3

    3

  • PERFECT SCORE BIOLOGY 2011

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