production of invert sugar using lentikats biotechnology
TRANSCRIPT
Production of Invert Sugar using Lentikats Biotechnology
Content
Our aim
What is Lentikats Biocatalyst
Production of Lentikats Biocatalyst
Benefits of Lentikats Biotechnology
Lentikats Biocatalyst in Food Industry
Our guarantees
Our partners
Contacts
LentiKat’s a.s. in Food Industry LentiKat’s a.s. follows up on nine year of research and development of
Lentikats Biotechnology. Our production know-how for commercial use of our Biocatalyst and
for its industrial applications is patented internationally. Our main focus is in intensification of production processes that leads
to:• higher process yields• enhanced process stability,• Increased revenue,• Reduction of environmental footprint.
Biotechnologies are changingthe world…
Lentikats BiotechnologyUnique shape of the matrix and original immobilisation procedure enables to encapsulate free enzymes or microorganisms (bacteria, yeasts, fungi) inside a hydrogel porous carrier from polyvinyl alcohol (PVA). The advantages of PVA matrix are:
• excellent physical and mechanical characteristics that result in a long-term mechanical stability
• hardly biodegradable• no toxic effects
Lentikats Biocatalysts enable easy separation of enzymes or microorganisms from the bulk media and therefore can be used repeatedly.
With biotechnologies to a better future…
How does the Lentikats Biocatalyst work?
A maximum surface in combination with the minimum volume of Lentikats Biocatalyst results in a substantial reduction of diffusion limitations associated with the transfer of substrate into the matrix and the product out of the matrix.
Why polyvinylalcohol?
• It is an inexpensive material
• Encapsulation takes place under optimal, cell-sparing conditions
• It allows a production of hydrogel with excellent mechanical properties
• It does not have any side effects on fermentation
• It offers high capacity for the cell loading
• The process is stable and easy to control
• It is a non-toxic material
Biotechnologies are changingthe world…
How is Lentikats Biocatalyst produced?
Lentikats Biocatalysts
• Biocatalyst Types for Use in Lentikats Encapsulation:• Free Cells
• dead cells• viable, non growing cells• viable and growing cells
• Free or Immobilised Enzymes.
Lentikats Biotechnology allows to immobilise any type of enzyme • The size of Lentikats Biocatalyst pores varies depending on the size and diameter of the Biocatalyst due to:
• PVA additive type (size of macromolecule)• additive concentration• biomass concentration
Lentikats Biocatalysts Manufacturing Plant
Laboratory Units for Encapsulation
• For low-capacity production of encapsulates (especially for research purposes), a laboratory device is available
What are the benefits?
Higher reaction rates
Reduction of reaction tanks’ volume
Operational costs savings (electricity, heat)
Lower production of waste and by-products
Environmentally friendly disposal
Matrix containing high concentration of biomass
Higher yields (in comparison to conventional technologies using free biomass)
Possibility of repeated usage
Operational costs savings
Enhanced resistance of modified and immobilised biomass
Higher process stability
Easy separation of biomass from reaction media
Reduced costs for downstream processes (separation)
..and also…
• Shortening of the reaction times• Much higher yields in comparison to conventional
technologies (up to 5 – 15 % higher)• Repetitive use of the Biocatalyst, easy separation from
media (sieve separator)• Higher productivity of the Biocatalyst• Lower risk of contamination during the production
process• High stability and easy control of production process
Biotechnologies are changing the world…
Technological set-up
- inlet of reaction media
- filling of Lentikats Biocatalyst
- medium with Lentikats Biocatalyst
- stirrer
- sieve separator
- outlet of separated media
- outlet of Lentikats Biocatalyst
Lentikats and food industry
We currently offer solutions for the following processes: production of lactose free milk
Lentikats Biocatalyst with β-galaktosidase activity
production of invert sugar Lentikats Biocatalyst with invertase activity
whey processingLentikats Biocatalyst with β-galaktosidase activity for:
• production of D-galaktose from whey lactose (pharmaceutical quality)• production of galacto-oligosaccharides from whey lactose
production of special syrupsLentikats Biocatalyst with invertase activity for production of glucose-fructose syrupsLentikats Biocatalyst with immobilised glucoamylase for production of glucose from sugar and starch-based materials or glucose syrups from maltotriose or maltose syrup
Lentikats and food industry
Following solutions are currently under development and testing:
production of organic acids Lentikats Biocatalyst with immobilised bacteria Bacillus coagulans for production of lactic acid from lactose, glucose and sucroseLentikats Biocatalyst with immobilised filamentous fungi Aspergillus niger for production of gluconic acid from sucrose and glucoseLentikats Biocatalyst with immobilised filamentous fungi Aspergillus terreus for production of itaconic acid from sucrose and glucose
Biotechnologies are changing the world…
Invert Sugar in Food Industry
@Sucrose source – sugar beat, sugar cane@Mixture of glucose and fructose (sucrose hydrolysis)@ Invert sugar as a product:
composition 33% sucrose, 35% glucose and 32% fructose » composition 4% sucrose, 51% glucose and 45% fructose invert sugar is more expensive than syrup» invert sugar is sweeter and contains also other
monosaccharides apart from sucrose large variety of applications: kids food, pastry, soups and
sauces, candy and sweets, fruit drinks, dairy products.
Invert sugar in Distilleries
@Sucrose source – sugar beat, sugar cane@Mixture of glucose and fructose@molasses – benefits of sucrose hydrolysis
a longer shelf-life » possibility of a year-round operation for molasses distilleriesa lower water binding capacity – possibility to a achieve
higher thickness prior to storage (80%)» lower demands for storage space and transportation
Lentikats Biotechnology and production of invert sugar
Lentikats Biocatalyst with invertase activity= immobilised saccharase (β-D-fructofuranosidase) saccharase source – Baker’s yeast (Candida utilis) invertase activity 600 - 1000 g sucrose/kg LB/hr optimum temperature 30 - 35°C optimum pH 4.5 – 5.0 operational stability 600 – 1000 hodin
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Lentikats Biotechnology and production of invert sugar
The active compound is immobilised invertase for sucrose hydrolysis to glucose and fructose.
- Standard activity assay:
» batch test» standard sucrose solution with the concentration 100 g/l» temperature 30°C» pH 4.5 (10 mM acetate buffer)» conversion time 50 min» conversion rate 100%
Case studies
1) Hydrolysis of sucrose solution
Input parameters sucrose concentration 450 g/l pH = 4.5 temperature = 30°C batch operation mode solution volume: 2 222 m3
Lentikats Biocatalyst» activity 1000 g SUC/kg LB/hr» stability 900 hr» amount 1 ton
Outlet concentration:
sucrose 10 g/l glucose 220 g/l fructose 220 g/l
volume 2 222 m3
Invert sugar production amount 1 000 000 kg
14 EUR/m3 solution 0.03 EUR/kg invert
sugar
Invert sugar production
2) Hydrolysis of sucrose solution
Input parameters sucrose concentration 450 g/l pH = 4.5 temperature = 30°C batch operation mode Solution volume: 2222 m3
Lentikats Biocatalyst» activity 1000 g SUC/kg LB/hr» stability 1000 hr» amount 1 ton
Output concentration:
sucrose 10 g/l glucose 220 g/l fructose 220 g/l
volume 2 222 m3
Ethanol production volume 615 333 litres a.a.
14 EUR/m3 solution 0.05 EUR/litre a.a.
Ethanol production
3) Molasses distillery
Input parameters sucrose concentration 120 g/l pH = 4.5 temperature = 30°C continuous operation mode molasses volume: 8 333 m3
Lentikats Biocatalyst» activity 1000 g SUC/kg LB/hr» stability 1000 hr» amount 1 ton
Output concentration:
sucrose 30 g/l glucose 45 g/l fructose 45 g/l
volume 8 333 m3
Ethanol production volume 629 167 litres a.a.
4 EUR/m3 molasses 0.05 EUR/litre a.a.
Ethanol production
Summary – application of 1 ton of LB
Sucrose solution – 450 g/l sucrose
Volume of solution processed[m3]
Ethanol produced[l a.a.]
Price[EUR/m3 SUC solution]
Price [Kč/litr a.a.]
1 ton LB 2222 615 333 14 0.05
Molasses – 120 g/l sucrose
Volume of molasses processed[m3]
Ethanol produced[l a.a.]
Price[EUR/m3 molasses]
Price [Kč/litr a.a.]
1 ton LB 8333 629 167 4 0.05
Investment and Investment Return Guarantees
• You can easily verify our great results by testing our Biocatalyst in your or other facilities with the material of your choice.
• Sample of Lentikats Biocatalyst free of charge
• Our company provides assistance and full technical support.
• Our company offers a long-term cooperation to companies, whose main concerns are reduced investment costs and maximal process profitability.
Biotechnologies change the world…
Investment and Investment Return Guarantees
We guarantee the following: output parameters
operational stability of Lentikats Biocatalyst and its activity and productivity
Our partners in the field of industrial applications
Fermenta Biotech, DIL Complex, Ghodbunder Road, Thane (West) - 400 610, Maharashtra, India
www.fermentabiotech.com
Protean, Pod Lesem 9, 373 16 Dobra Voda u Ceskych Budejovic, Czech Republic
www.protean.cz
Pharmix s.r.o., Na Sadkach 2798, 767 01 Kromeriz, Czech Republic
www.pharmix.cz
Our partners in the field of research and development
Fermenta Biotech, DIL Complex, Ghodbunder Road, Thane (West) - 400 610, Maharashtra, India
www.fermentabiotech.com
Institute of Microbiology, Academy of Science of the Czech RepublicVídeňská 1083, 142 20 Prague 4, Krč, Czech Republic
www.biomed.cas.cz
STU Bratislava, Fakulta chemické a potravinářské technologie, Ústav biotechnologie a potravinářství, Radlinského 9, 81237 Bratislava, Slovakia
www.stuba.sk
VŠCHT Praha, Ústav kvasné chemie a bioinženýrství, Technická 5, 166 28 Praguea 6 www.vscht.cz
University of Alberta, Edmonton Alberta, T6G 2J9 Canada
www.ualberta.ca
Masaryk University, National Centre for Biomolecular Research, Kamenice 5, 625 00 Brno-Bohunice
www.ncbr.chemi.muni.cz
Thank you for attentionCompany Headquarters:Office Building ARGOLentiKat‘s a.s.Evropska 846/176a160 00 Prague 6Czech Republic
Tel.: + 420 255 710 680Fax: + 420 255 710 699E-mail: [email protected]
Production plant:LentiKat‘s a.s.Pod Vinicí 83471 27 Stráž pod RalskemCzech Republic
Tel.: + 420 487 851 393Fax: + 420 487 851 399E-mail: [email protected]
Laboratory:INBIT LentiKat‘s a.s.Kamenice 34625 00 BrnoCzech Republic
Tel.: + 420 511 205 288Tel.: + 420 511 205 287