The Meridian Channels

Primo Vascular System

A compilation of Slides from the pioneers researching the meridian system with modern tools.

Oschman J, Streeter TWJA, Soh KS, Jones J, Langevin HM, Hp Mae Wan

The PrimoVascularSystem

KS Soh et al

Early pioneers Bong Han and Soh

Primo Vascular System

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Organ-Surface PVS duct (on the surface of organs)

Superficial PVS duct(Acupuncture meridian)

Superficial PVS Corpuscle(Acupoints)

skin : superficial PVS ducts network surface of organs : OSBHD inside vessels : intra-vascular

PVS

Intravascular PVS duct

Physiological Significance

New Circulation System Hyaluronic acid, Amino acid, Mono nucleotide, Hormone: Adrenalin/noradrenalin

Bonghan Granules (sanals) (Ф≈0.8~2.4μm) containing DNA Adult stem cells: cell therapy Natural “micro-cell”: gene therapy

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Primo vascular vessels

Primo Vascular/meridian Theory

Meridian line is not confined to skin, but ubiquitous.

Meridian is a concrete duct system through which the liquid containing the granules (Sanal :living granule in Korean) flows.

Sanals aggregate to form cells and cells disintegrated into Sanal.

Sanals function as totipotent stem cells.

Different substructures of PVS exist

PVS>Tubule>Striae (Sanals)

Staining the PVS

Acridine orange stain

Fig. 4  Immunohistochemical image of the skin sample of Fig 3. Staining was done by DAPI for nuclei(blue), anti-NF-M antibody for peripheral nerves(red), and RECA-1 antibody for blood vessels(green).One can

observe a peripheral nerve ending(PN) and a hair folicle(HF),and blood capillaries(C). Scale bar represents 100µm.

Stereomicroscopic view of PVS

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Fig.1. Stereomicroscopic Images of novel threadlike structures

The stereo-microscopic image of a threadlike structure stained by Acian blue (arrow heads).The solution of Alcian blue was injected into the

acupoint of ST36(arrow) on the rat skin. An Alcian-blue-stanined threadlike structure (arrow heads) was visualized and proceeded from ST36 point to

hypodermis.

Electron Microscope: clear view of the physical meridian

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SEM

Nanobiotechnology

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Flowing Channel of Nanoparticles

The stereo-microscopic image of a threadlike structure stained by Acian blue (arrow heads).The solution of Alcian blue was injected into the

acupoint of ST36(arrow) on the rat skin. An Alcian-blue-stanined threadlike structure (arrow heads) was visualized and proceeded from ST36 point to

hypodermis.

Fig. 4  Immunohistochemical image of the skin sample of Fig 3. Staining was done by DAPI for nuclei(blue), anti-NF-M antibody for peripheral nerves(red), and RECA-1 antibody for blood vessels(green).One can

observe a peripheral nerve ending(PN) and a hair folicle(HF),and blood capillaries(C). Scale bar represents 100µm.

In 1960’s Bong Han Kim showed that new tubular structures exist inside and outside of blood vessel and lymphatic vessel, and on the surface of internal organ as well as under the dermis, which he believed as the traditional meridian lines.

Ubiquitous presence of Primo Vascular System

PVS a closer look

Acridine orange stain

PVS and the circulatory system(blood vessel, extra-jugular vein)

Extravascular PVS (Lymphatic Vessel)

Organ Surface PVS

The Meridians – Primo Vascular System

Biofield Viewer image taken of left arm highlights flow of chi in Primo vascular system /meridian channels

•Pioneering experiments Voll – EAV Becker – DC potential over meridian. Pierre de Vernejoul - Injected radioactive tracer into acupoints

Traditional Oriental Medicine.

Fish bones/ Arrows

4500 years in development

365 points now about 2000 accepted

The primo Vascular System/ meridians are channels for the organs to communicate with the external environment

They provide the energy for homeostasis

The allow the transport of chi/prana to the organs

Your whole body becomes electrically connected to the earth through an acupuncture point on the ball of the foot called

Kidney 1. The meridians extend throughout your body.

Kidney 1

The kidney meridian begins at the ball of the foot

(1) and extends upward. An internal

branch from the kidney (8) connects

internally to the urinary bladder (9) and thence to the bladder meridian.

Subcutaneous tissue, unidirectional needle rotation.

ultrasound scanning acoustic microscopy

Same tissueFixed, embedded, sectioned,

stained

1 mm

Langevin, HM, et al, 2002. FASEB Journal

Acuvision-04 is a microprocessor device for medical diagnostics, based on visualisation of acupoints and is designed for primary screening of diseases. The device provides timely

diagnostics, non-invasive and harmless

Professor Joie JonesUniversity of California Irvine

Worked with Dr. Z.H.Cho in Dept. of Radiological Sciences

Used Functional Magnetic Resonance Imaging (fMRI)to correlate Brain activity with acupuncture points

Compared light flash stimulation to acupuncture stimulationin eliciting cortical response

Also used Ultrasound to stimulate acupuncture points andfound a much faster brain response than could be explained normal neuronal transmission.

Cho et al 1998 New finding of the correlation between acupoints and corresponding brain cortices using functional MRI. Proceedings of the National Academy of Sciences 95(5):2670-2673.

light acupuncture

Vision pointsbladder meridian

light sonopuncture 250 msec. 7 sec.

Vision pointsbladder meridian

Joie Jones

Sonopuncture is indistinguishablefrom needling.Two orders of magnitude fasterthan any known biological process.

Vision pointsbladder meridian

Joie Jones

At about 50 MHz there is enhancedattenuation at acupoints.

Attenuation is related to elasticity.

Proposed mechanical signal transduction and downstream effects of acupuncture needle manipulation at gross and microscopic levels.

Helene M Langevin and colleaguesVermont Medical College

Applies to all forms of touch therapy.

Langevin et al 2001 Biomechanical response to acupuncture needling in humans. Journal of Applied Physiology 91(6):2471-2478

Langevin et al 2001 Mechanical signaling through connective tissue: a mechanism for the therapeutic effects of acupuncture. FASCEB Journal 15(2):2275-2282

Langevin et al 2002 Evidence of connective tissue involvement in acupuncture. FASCEB Journal 16(8):872-876

Langevin HM, Yandow JA, 2002.  Relationship of acupuncturepoints and meridians to connective tissue planes. Anatomical Record 269:257-265

Langevin HM, Yandow JA, 2002.  Relationship of acupuncturepoints and meridians to connective tissue planes. Anatomical Record 269:257-265.    

Acupoints have a polygonal structure. Points rotate when needle is inserted.

Top and bottom halves twist in opposite directions.

Stimulation of point 67 causes proximal points

to rotate in sequence, with a

few seconds delay between

each point.

Proposed mechanical signal transduction and downstream effects of acupuncture needle manipulation at gross and microscopic levels. The lung meridian on the lateral border of the biceps is a good example and may coincide with some of the connective tissue planes.

“The acupoints feel like depressions that enable me to contact several fascial layers at once. It feels like putting your finger into a cone. Initially there is more tension in the tissue when you try to twist in one direction compared to the opposite direction. After the tissue has released, the resistance to twisting is the same in both directions.”

Stanley Rosenberg, Denmark

Waves traveling down a Coaxial Cable

These are actually type 2 waves, if ends are not properly terminated they radiate scalar waves

f MRI Assessment of Acupuncture

Radiographic studies using scintiphotography: a

radioactive dye, technetium-99, injected

into a meridian, flow linearly along the meridian pathway and well up into

small circles at the classical acupuncture

points. Darras JC, de Vernejoul P, Albarede P, et al. Nuclear medicine investigation of

transmission of acupuncture information. Acupuncture Med. 1993;

11:22-28.

Stomach meridian after moxibustion near left shoulder

blade.

Bladdermeridian

after moxibusti

on

In every culture and in every medical tradition before ours, healing was accomplished by moving energy.

•Albert Szent-Györgyi

Acupuncture meridians

Primary channels through the living matrixHigh speed communicationReach into every part of the organism

Atomic Force Microscope

Bonghan microcell cycle (Bonghan Kim, 1965)-69-

(1) All the morphological components of organism are ceaselessly reproduced.

(2) The self-reproduction of organism takes the form of Bonghan microcell-cell cycles.

(3) The self-reproduction of organism is performed by the acupuncture-meridian system.

Theory of Bonghan Microcell

AFM image of Bonghan microcell

A Topography, B Error, C Magnified of B, D line profile

1.4 um x 1um x 350nm sized oval Bonghan microcell. It shows clearly unique surface structure of layers and portions (B & C) whose size is from 0.2um to 0.6 um in diameter. The line profile analysis shows that the common patterns on the surface are steps of about 20nm height. This pattern is thought to be revealing the stereotype of its membrane structure. -70-20nm

A B C

D

Sanals by Acridine Orange

Anemia induced method, PHD inj. for 3 days.Rabbit, female, 2.5kg, 10weeksX1000, scale bar=10um

Centrifugal separation method, acridine orange

Model of Sanal cycle by Bong Han Kim

Sanals and Cell interconverted?

Observation of Sanals

Sanal observation by TEM

Sanals in Primo Vascular System

Two Sanals stacked?

Sanals proliferate by budding?