presented by rachel barlow

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Study to Refine Comal Springs Riffle Beetle Collection Methods and Establish Riffle Beetle Population Distribution Presented by Rachel Barlow

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Study to Refine Comal Springs Riffle Beetle Collection Methods and Establish Riffle Beetle Population Distribution. Presented by Rachel Barlow. According to the EAA Request for Proposals (RFP ) for this study: Comal Springs riffle beetle (CSRB) distribution is currently unknown - PowerPoint PPT Presentation

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Page 1: Presented by Rachel Barlow

Study to Refine Comal Springs Riffle Beetle Collection Methods and

Establish Riffle Beetle Population Distribution

Presented by Rachel Barlow

Page 2: Presented by Rachel Barlow

According to the EAA Request for Proposals (RFP) for this study:Comal Springs riffle beetle (CSRB) distribution is currently

unknownCSRB collection methods can be improved

Per the RFP, this study is intended to:Establish a distribution of springs serving as CSRB habitatTest the success rate of at least three collection methodsCollect CSRB for use in other 2014 Applied Research studies

Page 3: Presented by Rachel Barlow

425 mapped springs in Landa Lake and the Comal System (Norris and Gibson 2013)

Page 4: Presented by Rachel Barlow

Currently Understood CSRB Distribution within the Comal System

Western shorelineOff northeastern tip of Spring IslandBetween western shoreline and northernmost peninsula

of Pecan IslandSpring runs 1-3

Page 5: Presented by Rachel Barlow

BIO-WEST 2002

Page 6: Presented by Rachel Barlow

BIO-WEST 2002

Page 7: Presented by Rachel Barlow

Previous Collection Methods

Fingers and/or soft forcepsDip netsCotton cloth luresCotton lures inside PVC

tubingDrift net sampling

Cotton cloth lure allowed to “culture” in situ for a period of four weeks

Page 8: Presented by Rachel Barlow

Study ObjectivesTest the success rate of three lure typesEstablish CSRB distribution within the Comal System

Page 9: Presented by Rachel Barlow

Methodology

Preliminary trials with surrogate speciesCollect 250 CSRB (maximum)Store in aquaria

Beetle Collection and Housing

Page 10: Presented by Rachel Barlow

Methodology (con’t)

Four experimental chambers- Three test systems- Control

Treatments (24 hour test cycle x 3)- Cotton cloth lure- Lighted cotton cloth lure - Hemp cloth lure

50 CSRB per chamberExpected results and analysis

- % CSRB captured per test cycle- ANOVA (lure type)

Laboratory Experimental Design

Page 11: Presented by Rachel Barlow

Methodology (con’t)

Adaptive sampling- Only sample springs with CSRB habitat- CSRB present, move on

Exclude springs with known CSRBUse most effective lure realized in laboratory studySurvey (check traps) up to 3 times Survey no more than twice weekly

Population Distribution Study

Page 12: Presented by Rachel Barlow

CSRB Habitat Criteria(based on physical parameters measured by Norris and Gibson 2013)

Spring type: upwellingTemperature: 68-75°FSubstrate consisting of organics, sand, course sand,

very small gravel, small gravel, medium gravel, rubble, small cobble, and large cobble

Flow sufficient to maintain clear substrate conditions

Page 13: Presented by Rachel Barlow

Expected Results

Tested collection methodUpdated map of CSRB distributionAnimals per trap across spring orificesExamination of other contributing parametersNumber of surveys leading to presence/absence

determinationNature of changing spring locations in relation to

presence/absence studies

Page 14: Presented by Rachel Barlow