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PRACTICAS DE LABORATORIO EN REPRODUCCION INVESTIGACION EN REPRODUCCION Slide 2 HISTOLOGIA Gonzales GF, Hartinger S, Flores Y, Zrate K, Yi P. Cycloheximide prevents production of arresting, a fraction of 30-50 kDa obtained from seminiferous tubules conditioned medium: An evidence that this fraction is produced in vitro. Asian Journal of Andrology. 2004; 6: 359-364. Gonzales GF, Miranda S, Nieto J, Fernandez G, Yucra S, Rubio J, Yi P, Gasco M. Red Maca (Lepidium meyenii) reduced prostate size in rats. Reproductive Biology and Endocrinology. 2005; 3(1):5 Slide 3 Izquierda: Control Derecha: Arresting Slide 4 Control Paracrino Hedger MP, Khatab S, Gonzales GF, de Kretser DM. Acute and short term actions of serotonin administration on the pituitary-testicular axis in the adult rat. Reproduction, Fertility and Development. 1995;7: 1101-1109. Slide 5 ESPERMATOGENESIS Gonzales GF, Gasco M, Crdova A, Chung A, Rubio J, Villegas L. Effect of Lepidium meyenii (Maca) on spermatogenesis in male rats acutely exposed to high altitude (4340 m). Journal of Endocrinology 2004, 180:87-95 Slide 6 TRANSILUMINACION Gonzales GF, Del Valle L. Adult rat seminiferous tubules secrete a fraction greater 30 KDa to regulate spermatogenesis. Human Reproduction 1995;10: 1435-1443. Almenara A., Escalante G., Gazzo E., Gonzales GF. Transillumination to evaluate spermatogenesis: Effect of testosterone enanthate in adult male rats. Archives of Andrology. 2001; 46:21-27. Gonzales GF, Ruiz A, Gonzales C, Villegas L, Crdova A. Effect of Lepidium meyenii (Maca) roots, a Peruvian plant on spermatogenesis of male rats. Asian Journal of Andrology. 2001; 3:231-233. Gonzales GF, Hartinger S, Flores Y, Zrate K, Yi P. Cycloheximide prevents production of arresting, a fraction of 30-50 kDa obtained from seminiferous tubules conditioned medium: An evidence that this fraction is produced in vitro. Asian Journal of Andrology. 2004; 6: 359-364. Chung F, Rubio J, Gonzales C, Gasco M, Gonzales GF. Dose-response effects of Lepidium meyenii (Maca) aqueous extract on testicular function and weight of different organs in adult rats. Journal of Ethnopharmacology. 2005; 98:143-147. Slide 7 CONTEO DE ESPERMATIDES EN TESTICULO Gonzales C, Rubio J, Gasco M, Nieto J, Yucra S, Gonzales GF. Effect of short- term and long-term treatments with three ecotypes of Lepidium meyenii (MACA) on spermatogenesis in rats. Journal of Ethnopharmacology. 2005. En prensa. Slide 8 CONTEO Y MOVILIDAD DE ESPERMATOZOIDES EN EPIDIDIMO Del Valle LJ, Bustos-Obregn E, Gonzales GF. Serum reproductive hormone levels and sperm production in male adult rats after treatment with arresting, a fraction obtained from seminiferous tubules conditioned medium. Andrologia 2003; 35: 351-357. Gasco M, Rubio J, Chung A, Villegas L, Gonzales GF. Effect of high altitude exposure on spermatogenesis and epididymal sperm count in male rats. Andrologia 2003, 35: 368-374. Gonzales GF, Rubio J, Chung A, Gasco M, Villegas L. Effect of alcoholic extract of Lepidium meyenii (Maca) on testicular function of male rats. Asian Journal of Andrology 2003; 5: 349-352 Gonzales GF, Gasco M, Crdova A, Chung A, Rubio J, Villegas L. Effect of Lepidium meyenii (Maca) on spermatogenesis in male rats acutely exposed to high altitude (4340 m). Journal of Endocrinology 2004, 180:87-95 Slide 9 Epididymal sperm count (106) in male rats treated for 7 and 42 days with vehicle, Red Maca, Yellow Maca or Black Maca. *P