Introduction: The decision of a woman to get pregnant atlater age of her reproductive cycle has be a phenomenonaround the world. Epidemiology data show frequentincrease of clinical complications in direct proportion inadvanced age motherhood, hypertensive disturbances beingmore prevalent.

Objectives: Analyse the prevalence of hypertensive syn-dromes in pre-determined age groups at Hospital GuilhermeÁlvaro in Santos, São Paulo, Brazil showing the different seg-ments in each one.

Methods: From data collected in the outpatient depart-ment of Hospital Guilherme Álvaro of High Risk Pre-natalbetween 04/06/2008 and 30/05/2011, a prevalence transver-sal study was carried out where data were obtained from628 patients aged between 16 and 46 years. Procedures ofhomogeny analysts were set out, always collecting data suchas age and disorder for high risk gestation. According to age,patients were divided into groups: precocious (up to19 years old), middle age (between 20 and 34) and late preg-nancies (over 35).

Results: In the precocious pregnancies, clinical illnesses/no hypertension (31%) were observed in first place, 25% (8)twin pregnancy in second place, 19% (6) showed hyperten-sive disturbances. Concerning pregnancies between 20 and34 years old, 36% (144 patients) showed hypertensive syn-dromes, 23% (92 patients) showed endocrine disturbances,22% (90 patients) showed clinical illnesses/no hypertension,and 9% twin pregnancy. Regarding late pregnancies, themost frequent disturbance was isolated hypertensive syn-dromes: 44% (88 patients) in first place, only endocrine dis-turbances, 24% (47 patients) in second place followed byassociation between hypertensive syndromes and endocri-nopathy with 13% (26 patients).

Conclusion: About precocious pregnancies, greater preva-lence showed clinical illnesses/no hypertension, whereasmiddle age and late pregnancies showed greater hypertensivesyndrome prevalence, results, which are compatible withother studies, have been observed that due to advance ofage, hypertensive syndromes are more frequent. For late preg-nancies, the prevalence of clinical illnesses/no hypertensionwas a lower percentage regarding the other two groups: pre-cocious pregnancies (31% = 10 women), middle age pregnan-cies (22% = 90 women), and late pregnancies (2% = 7 women).

Considering the fact that the occurrence of pregnancy ismore and more late in life, it can be concluded that the pro-fessionals must be prepared to attend pregnancies onwomen with hypertensive disturbances and their eventualcomplications.

Disclosure of interest: None declared.

doi:10.1016/j.preghy.2012.04.258

PP148. Is there podocyturia in pregnant women withchronic hypertension? (Preliminary results)F.B. Roberto 1, T.A. Facca 1, A.R.P.R. Pereira 2, J.L. Sato 1,M.R. Mesquita 1, S.K. Nishida 2, F.L.P. Sousa 1, A.F. Moron 1,V.P. Teixeira 2, G.M.M. Kirsztajn 2, N. Sass 1,* (1 Obstetrics,

Federal University of São Paulo, São Paulo, Brazil,2 Nephrology, Federal University of São Paulo, São Paulo,Brazil)

Introduction: The severity of renal injury is directlyrelated to stages of the hypertensive disease. In pregnancy,the glomerulopathy seems to be much more serious whenthe woman has pre-existing hypertension or chronic kidneydisease. Recently, some studies have demonstrated that therenal damage installed during pregnancy may be irreversibleand it may emerge clinically years later.

Objectives: Identify the excretion of podocytes in theurine of pregnant women with chronic hypertension.

Methods: Podocyturia were evaluated in 12 women in thethird trimester of pregnancy with chronic hypertension bycytospin method using rabbit anti-podocin antibody and goatanti-rabbit IgG-FITCas secondary antibody. The slides werephotographed in 30 fields by indirect immunofluorescencemicroscopy, we used a semiquantitative criterion for itsidentification (1–10 podocytes cells: +, 10–20: ++, > 20:+++). (Grant FAPESP 08/56338-1)

Results: Average of age and gestational age were33.83 years and 34.74 weeks, respectively. The average ofblood pressure was 120 � 80 mmHg. The analysis of podo-cyturia in women with pre-existing hypertension had no sta-tistical significance. The urinary excretion of podocyte cellsin pregnant women affected by chronic hypertension seemedto behave similarly to healthy pregnancy, as we had previ-ously demonstrated. A significant level of podocyturia wasdetected in only one hypertensive patient (see Table 1).

Conclusion: So far our study does not allow consistentfindings about podocyturia in pre-existing hypertensivepregnant women. Higher levels of podocyturia may not bedetected in these group of pregnant women, although largerstudies are needed to determine whether the chronic hyper-tensive status result or not in a significant kidney damage inpregnancy and if differential follow-up is needed to attenu-ate the development of renal damage.

Disclosure of interest: None declared.

doi:10.1016/j.preghy.2012.04.259

PP149. Hypertensive risk factors: Do they influencepregnancy outcome in women affected by new onset pre-eclampsia?V. Gerosa, M.E. Gregorini, G. Pagani, P.L. Rovida,A. Lojacono *, A. Valcamonico, T. Frusca (Maternal FetalMedicine Unit, Dept. Obstetrics and Gynecology, Universityof Brescia, Brescia, Italy)

Table 1Semiquantitative detection of podocyturia by indirect immunofluorescencemicroscopy.

Slide Podocyturia Slide Podocyturia

1 + 7 +2 +++ 8 ++3 + 9 +4 ++ 10 ++5 + 11 +6 + 12 –

Abstracts / Pregnancy Hypertension: An International Journal of Women’s Cardiovascular Health 2 (2012) 240–339 319