Methods: This was a cross sectional study including 185women with normal singleton pregnancies at 11–40 weeksof gestation and 49 non-pregnant controls. Stroke volume(SV), CO and SVR were measured using the USCOM device.All measurements were performed with the patients insupine position. All women with a gestational age of>20 weeks were in a left lateral position by placing awedge-shaped pillow under their right side to prevent venacava compression. In a group of 25 pregnant women, eachmeasurement was repeated three times to evaluate thereproducibility of this technique. Cardiac index (CI), SVindex (SVI) and SVR index (SVRI) relate CO, SV and SVR tothe body surface area. The data were normally distributedafter logarithmic transformation. Comparisons betweenpregnant and non-pregnant women were performed usingStudentt-test, Chi-Square test or multiple regression analy-sis, when adjustment for potential confounders was neces-sary. Data analysis was performed using SPSS 16.0.

Results: In the first trimester, all of the following vascularparameters were higher in pregnant women compared tonon-pregnant controls: CO [median (IQR): 4.86 (4.45–5.57)vs 5.57 (4.76–6.52) L/min, P < 0.001], CI [median (IQR): 2.69(2.44–3.07) vs 3.25 (2.80–3.86) L/min/m2, P < 0.001], SV[median (IQR): 72.51 (68.10–80.18) vs 80.75 (74.50–99.74) mL/beat, P < 0.001], SVI [median (IQR): 41.93 (37.53–46.57) vs 47.01 (43.85–53.79) mL/m2/beat, P < 0.001]. Preg-nant women had significantly lower SVR [median (IQR):1458 (1261–1649) vs 1165 (1023–1406) sec/cm�5,P < 0.001] and SVRI [median (IQR): 2646 (2307–2963) vs2006 (1179–2277) dynes-sec/cm�5/m2, P < 0.001] at 11–13 weeks’ gestation.

Conclusion: Using USCOM, maternal cardiac function canbe assessed in a simple, non-invasive and reproducible man-ner. This simple technique is likely to facilitate large scalestudies of maternal cardiovascular function in pregnancy.

Disclosure of interest: None declared

doi:10.1016/j.preghy.2012.04.208

PP098. Lipidic fingerprinting in women with early-onsetpreeclampsia: A first lookL. De Oliveira 1,2,*, H. Korkes 1,2, E. Lo Turco 1, R. Bertola 3,N. Sass 1,2, T. Bonetti 3, A.F. Moron 1, I.D. Da Silva 4

(1 Obstetrics Department, Federal University of São Paulo,Sao Paulo, Brazil, 2 Obstetrics Department, School MaternityVila Nova Cachoeirinha, Sao Paulo, Brazil, 3 UrologicDepartment, Federal University of São Paulo, Sao Paulo,Brazil, 4 Gynecologic Department, Federal University of SãoPaulo, Sao Paulo, Brazil)

Introduction: preeclampsia is characterized by intenseinflammatory response and an anti-angiogenic state. Mater-nal obesity has been considered to have important impacton the genesis of preeclampsia as lipotoxicity leads tomaternal endothelial dysfunction and chronic inflammation.Here we investigate the plasma lipid profile of preeclampticwomen.

Objectives: identify possible lipid biomarkers forpreeclampsia.

Methods: this study included 8 pregnant women withearly-onset preeclampsia (before 34 weeks gestation) and8 normal pregnant women. Each patient in the preeclampsiagroup was matched to a patient in the control group accord-ing to gestational age at the time of sample collection. Allpatients in the control group were followed until term andhad normal outcomes. To investigate the lipid profile, lipidswere extracted from plasma samples using the Bligh-Dyerprotocol and the extracts were subjected to MALDI-TOFMass Spectrometry. Data matrix was exported for partialleast squares discriminant analysis. All the variablesanalysed were sorted by a score number named VariableImportance in the Projection. The major discriminant vari-ables were selected and underwent to Mann–Whitney Utest.

Results: a total of 1290 ions were initially identified dur-ing lipidomic assessment. Twelve m/z signals were high-lighted as the most important lipids for the discriminationof patients with preeclampsia. The identification of thesedifferential lipids was carried out through Lipid DatabaseSearch. The main classes identified were Glycerophosphoch-olines [GP01], Glycerophosphoserines [GP03], Glycerophos-phoglycerols [GP04], Glycosyldiradylglycerols [GL05] andGlycerophosphates [GP10].

Conclusion: Our results suggest that some lipid speciesmay be potential biomarkers for early-onset preeclampsia.

Disclosure of interest: None declared

doi:10.1016/j.preghy.2012.04.209

PP099. Chronic hypertension: follow-up of chronichypertension pregnant women in 2008–2009A.F. Caeiro *, V. Santos, G. Dias, E. Landim, I. Santos, T. Matos,A. Nazaré (Obstetrics and Gynecology, Hospital FernandoFonseca, Lisbon, Portugal)

Introduction: Chronic Hypertension (CH) is one of themost prevalent diseases in the world. Because women aregetting pregnant in late ages, the incidence of ChronicHypertension in pregnancy is raising (2–3%). As a long termdisease in its essential form, with low repercussion in target-organs at reproductive age, the medical complicationsrelated to CH are not the main concern of the pregnantwomen. However, it influences the pregnancy and mayresult in serious maternal-fetal complications, especially ifa preeclampsia/eclampsia superimposes to pregnancy. Thecomplications may be manifested as placental abruption,acute renal failure, cardiac decompensation, and cerebralaccidents in the mother and of growth restriction and unex-plained mid-trimester fetal death. The main goal in the fol-low-up of this pregnant women is to achieve controlledlevels of blood pressure (Systolic – 110–140 mm Hg, Dia-stolic – 80–90 mm Hg) with anti-hypertensive drugs, andevaluate and early diagnose the most serious complication,preeclampsia/eclampsia(PE/E).

Objectives: Review the cases of Chronic Hypertension fol-lowed in our institution in 2008–2009, with the analysis of

Abstracts / Pregnancy Hypertension: An International Journal of Women’s Cardiovascular Health 2 (2012) 240–339 293

population, anti-hypertensive drugs required to control HTA,fetal complications (growth restriction, preterm deliveryand fetal death) and maternal/fetal complications (PE/E,Abruption placentae, Renal and Cardiac acute insufficiency,and cerebral accidents).

Methods: In a retrospective study, from January 2008 toDecember 2009, were analysed all files related with ChronicHypertension followed in the obstetric department of Prof.Fernando Fonseca’s Hospital. The statistic analysis wasbased on Excel 2007.

Results: The incidence of Chronic Hypertension was 40% ofall hypertensive disorders in this period and of 1,5% in over-all pregnant population. The maternal mean age of thisgroup was 33,8 years (16;44) being the most prevalentpre-existing co-morbidities the endocrinologic disorders(obesity, Diabetes and thyroid pathology). 38% were previ-ous medicated with anti-hypertensive drugs, the majorityof them needing only one drug to control blood pressure(BP) (64%). During the pregnancy follow up, 78% were med-icated with one or more anti-hypertensive drugs, requiringonly one drug to control BP in the majority of cases 66%.In terms of fetal complications 3 cases of growth restrictionwere signed (2%), preterm delivery in 26 cases (21%) and 2interruptions of pregnancy in the second trimester before24 weeks because of maternal complications (2%). Therewas one case of Abruption Placentae (1%). In terms of mater-nal complications there were 5 ICU internments (4%), andone situation of acute cardiac disfunction (1%). The CH wascomplicated by PE/E in 22 cases (18%).

Conclusion: Chronic Hypertension is a prevalent disease,with an elevated incidence in pregnancy, and potentialmajor fetal-maternal complications, obliging medical pro-fessionals to a straight and careful follow-up to control BPlevels and early diagnose the adversely outcomes.

Disclosure of interest: None declared

doi:10.1016/j.preghy.2012.04.210

PP100. Pre-eclampsia: Risk factors and outcomes – A two-year studyA.V. Santos *, F. Caeiro, G. Dias, E. Landim, J. Fonseca,I. Santos, T. Matos, A. Nazaré (Obstetrics and Gynecology,Hospital Fernando Fonseca, Varzea de Sintra, Lisbon,Portugal)

Introduction: Pre-eclampsia (PE) is a hypertensive disor-der responsible for major morbidity and mortality inboth mother and fetus. There are some risk factors associ-ated with this entity, but it remains very difficult topredict.

Objectives: Study the incidence of PE and the related riskfactors, as well as the maternal and fetal outcome.

Methods: We reviewed the clinical records of pregnantwomen admitted to Prof. Fernando Fonseca’s Hospitalfrom January 2008 to December 2009, with the diagnosisof pre-eclampsia. The statistic analysis was based on Excel2007.

Results: There were 90 cases of PE, among the 308 hyper-tensive disorders reviewed, with an incidence of 1,1% in

overall population of pregnant women. Risk factors withhigher association were Chronic Hypertension before preg-nancy (24,4%), maternal age above 35 years old (16,67%),maternal age under 20 years old (14,44%), and previous epi-sode of pre-eclampsia (8,89%). Major maternal complica-tions that determined Intensive Care Unit admission wererecorded in 17 cases (18,89%), with 3 HELLP syndromes(Hemolysis, elevated liver enzymes, and low plate-lets)(3,33%). No maternal death was recorded. Pretermdelivery (PTD) was seen in 61,1%, 32% before 34 weeks and6,67% before 28 weeks. There were 19 cases of 1st minuteApgar Index below 7 and 5 cases of 5th minute Apgar Indexbelow 7.

There was one in utero death and two interruptions ofpregnancy below 24 weeks due to serious PE.

Three twin pregnancies.Conclusions: PE is a form of hypertensive pregnancy disor-

der, with a risk of recurrence in subsequent pregnancies. Ithas a catastrophic potential, mainly associated to PTD, andalso with significant morbidity to the pregnant women,reflected in the incidence of admissions to ICU, HELLP syn-drome and end-organ failure. In our study we confirmedthe adverse outcomes related to this entity, and the risk fac-tors associated.

Disclosure of interest: None declared

doi:10.1016/j.preghy.2012.04.211

PP101. Preeclampsia and pregnancy induced hyperten-sion and carotid artery atherosclerosisN. Sharashkina *, N. Runikhina, O. Tkacheva, I. Novikova(V.I.Kulakov Research Center for Obstetrics, Gynecology andPerinatology, Moscow, Russian Federation)

Introduction: Recent studies indicated preeclampsia (PE)and pregnancy-induced hypertension (PIH) as risk factorsfor cardiovascular diseases in young women. Women witha history of preeclampsia face double the risk of a heart dis-ease during the 5–15 years after pregnancy. We assessed theimpact of these factors on endothelial function, atheroscle-rotic changes and lipid metabolism in young women witha history of preeclampsia and pregnancy-inducedhypertension.

Objectives: The aim of this study was to examine whetherwomen with a history of preeclampsia more often showsigns of atherosclerosis compared with control group

Methods: We analyzed serum levels of total cholesterol(TC), HDL-C, LDL-C and triglycerides (TG). Endothelium-dependent vasodilation and carotid artery intima-mediathickness (CA-IMT) were evaluated in 18 patients with a his-tory of PE, 16 with a history of PIH and 17 healthy controls(CN). Inter-group differences were calculated using Stu-dent’s t-test.

Results: We found a worse lipid profile among womenwith PE and PIH. LDL-C was increased significantly in PEand PIH (PE: 3.17 mmol/l [SD 0.50] and PIH: 3.37 mmol/l[SD 0.48] vs CN: 2.83 mmol/l [SD 0.35], p < 0.05); TC andTG were higher in the PE and PIH groups, but not signifi-

294 Abstracts / Pregnancy Hypertension: An International Journal of Women’s Cardiovascular Health 2 (2012) 240–339