PowerPoint PortfolioPowerPoint Portfolio

By John A TranBy John A Tran714-913-3531714-913-3531

www.johnatran.comwww.johnatran.cominfo@johnatran.cominfo@johnatran.com

About MeAbout MeEducationEducation

– Pharmacology Ph.D, 2008Pharmacology Ph.D, 2008University of California, IrvineUniversity of California, Irvine

– Pharmacological Chemistry B.S., 2002Pharmacological Chemistry B.S., 2002University of California, San DiegoUniversity of California, San Diego

SkillsSkills– Published articles in reputable scientific Published articles in reputable scientific

journals on research findingsjournals on research findings

– Presented posters and slide presentations Presented posters and slide presentations garnering praise and earning prestigious garnering praise and earning prestigious awardsawards

– Computer savvyComputer savvyMicrosoft Office – especially PowerPoint Microsoft Office – especially PowerPoint (animations)(animations)EndNote – reference managerEndNote – reference managerWeb research – PubMed, Google, etcWeb research – PubMed, Google, etc

– A hard worker with the mentality to always get A hard worker with the mentality to always get the job done producing quality workthe job done producing quality work

Samples of My PowerPoint Samples of My PowerPoint WorkWork

Receptor TheoryReceptor Theory

Basic pharmacological principlesBasic pharmacological principles– Drug (agonist) binding to a receptor leading to Drug (agonist) binding to a receptor leading to

the activation of response in a cellthe activation of response in a cell

Receptor TheoryReceptor Theory

Stimulus – Agonist Stimulus – Agonist binding and receptor binding and receptor activationactivation

Transducer – Transducer – downstream signaling downstream signaling mechanismsmechanisms

Response – result of Response – result of receptor activation receptor activation that is measuredthat is measured

Cell

Characterization of Agonist ActivityCharacterization of Agonist Activity

Estimation of agonist properties from a Estimation of agonist properties from a response plotted on an agonist-response response plotted on an agonist-response curvecurve

Characterization of Agonist ActivityCharacterization of Agonist Activity

ECEC5050 – concentration – concentration

of agonist that elicits of agonist that elicits half-maximal half-maximal responseresponse

EEmaxmax – maximal – maximal

responseresponse

CHO-MCHO-M22 Cells Cells

Emphasizing the first panel (upper left) Emphasizing the first panel (upper left) during a presentationduring a presentation

CHO-MCHO-M22 cells cells

John A Tran et al (2008) Estimation of Relative Microscopic Affinity Constants of Agonists for the Active State of the Receptor in Functional Studies on M2 and M3 Receptors. Mol Pharmacol. (in press)

G Protein SignalingG Protein Signaling

Agonist bound to receptor may induce Agonist bound to receptor may induce signaling through one or two G proteins in signaling through one or two G proteins in a cella cell

G1 G2

MM33 KO and M KO and M22 KO KO

Using a box to highlight the panel that is Using a box to highlight the panel that is discussed and its transitiondiscussed and its transition

M2/M3 KO

M2 KO

M2 KO

John A Tran et al (2008) Estimation of Relative Microscopic Affinity Constants of Agonists for the Active State of the Receptor in Functional Studies on M2 and M3 Receptors. Mol Pharmacol. (in press)

Cause and Effect – Cause and Effect – & R ReceptorsReceptors

Arrow Diagram: Shows the inhibition of Arrow Diagram: Shows the inhibition of one of two one of two receptors receptors and the and the

consequential increase in contractionconsequential increase in contraction

Pictorial Diagram: Shows the decrease in Pictorial Diagram: Shows the decrease in response in a cell after the disappearance response in a cell after the disappearance of the R1 receptorof the R1 receptor

1

3(-)

(+)

Contraction

X

R1R2

SummarySummary

A unique way to summarize the A unique way to summarize the presentationpresentation

SummarySummary

The RAThe RAi i is a relative measure is a relative measure of microscopic affinity of microscopic affinity constants of the active state of constants of the active state of the receptorthe receptor

The RAThe RAii is the product of is the product of affinity and efficacy of the test affinity and efficacy of the test agonist relative to a standard agonist relative to a standard agonistagonist

The The RCRC analysis can be used analysis can be used in knockout studies to in knockout studies to determine the contribution of determine the contribution of various receptor subtypes to various receptor subtypes to the response measuredthe response measured

XX

YYi K

KRA

/1

/1

150max

501max2

KOWT

WTKO

ECE

ECERC

The EndThe End

This is small sampling of what is possible with This is small sampling of what is possible with Microsoft PowerPointMicrosoft PowerPointI hope this provides you with an idea of my skill I hope this provides you with an idea of my skill setsetAny questions? Please contact:Any questions? Please contact:

John A TranJohn A Tran714-913-3531714-913-3531

www.johnatran.comwww.johnatran.cominfo@johnatran.cominfo@johnatran.com