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  • Hindawi Publishing CorporationEvidence-Based Complementary and Alternative MedicineVolume 2013, Article ID 671252, 9 pageshttp://dx.doi.org/10.1155/2013/671252

    Research ArticlePolysaccharides-Rich Extract of Ganoderma lucidum(M.A. Curtis:Fr.) P. Karst Accelerates WoundHealing in Streptozotocin-Induced Diabetic Rats

    Poh-Guat Cheng,1,2 Chia-Wei Phan,1,2 Vikineswary Sabaratnam,1,2 Noorlidah Abdullah,1,2

    Mahmood Ameen Abdulla,1,3 and Umah Rani Kuppusamy1,3

    1 Mushroom Research Centre, University of Malaya, 50603 Kuala Lumpur, Malaysia2 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia3 Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia

    Correspondence should be addressed to Vikineswary Sabaratnam; viki@um.edu.my

    Received 2 June 2013; Revised 8 October 2013; Accepted 8 October 2013

    Academic Editor: Mohamed Eddouks

    Copyright 2013 Poh-Guat Cheng et al. This is an open access article distributed under the Creative Commons AttributionLicense, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properlycited.

    Ganoderma lucidum (M.A. Curtis:Fr.) P. Karst is a popular medicinal mushroom. Scientific reports had shown that the woundhealing effects ofG. lucidumwere partly attributed to its rich polysaccharides. However, little attention has been paid to its potentialeffects onwounds associated with diabetesmellitus. In this study, we evaluated the wound healing activity of the hot aqueous extractofG. lucidum in streptozotocin-induced diabetic rats.The extract ofG. lucidumwas standardised based on chemical contents (w/w)of total polysaccharides (25.1%), ganoderic acid A (0.45%), and adenosine (0.069%). Six groups of six rats were experimentallywounded in the posterior neck region. Intrasite gel was used as a positive control and aqueous cream as the placebo. Topicalapplication with 10% (w/w) of mushroom extract-incorporated aqueous cream was more effective than that with Intrasite gel interms of wound closure.The antioxidant activity in serumof rats treatedwith aqueous extract ofG. lucidumwas significantly higher;whereas the oxidative protein products and lipid damagewere lowerwhen compared to those of the controls.These findings stronglysupport the beneficial effects of standardised aqueous extract ofG. lucidum in acceleratingwoundhealing in streptozotocin-induceddiabetic rats.

    1. Introduction

    Diabetes mellitus is a metabolic disorder characterised byhyperglycemia with impaired carbohydrate, fat, and proteinmetabolism. The diabetic condition can be due to defects ininsulin secretion, action, or both [1]. It affects more than 180million individuals worldwide and by 2030 these numbers areprojected to double [2]. Diabetes mellitus has led to debili-tating consequences such as vasculopathy, retinopathy, andneuropathy [1]. More than 80% of diabetes mellitus is Type 2diabetes (noninsulin dependent) characterised by peripheralresistance to the action of insulin and decreased peripheralglucose uptake or increased hepatic glucose output [1].

    According to the statistics provided by the NationalDiabetes Information Clearinghouse, 15% of diabetic indi-viduals suffered from diabetic foot ulcers that caused lower

    limbs to be amputated [3].Thehyperglycemic state in diabeticpatients, especially those with peripheral vasculopathy in-terrupts proper wound healing. Wound healing occursas a cellular response to injury and involves activation ofkeratinocytes, fibroblasts, endothelial cells, macrophages,and platelets. Many growth factors and cytokines releasedby these cell types are needed to coordinate and maintainhealing [1, 4]. Furthermore, poor blood circulation and theoxygen supply in the affected area could lead to infectionand gangrene formation [5]. This often leads to increasedmorbidity andmortality. Despite the existence of protocols tostandardise wound care, the physiological impairments thatcan result in a diabetic foot ulcer (DFU) further complicatethe healing process [6]. Currently, the wounds in diabeticpatients are managed with antiseptic or antibiotic creams or

  • 2 Evidence-Based Complementary and Alternative Medicine

    gels such as Intrasite gel for wound dressing. Wound healingtakes prolonged periods and the healed wounds leave scars.In the search and discovery of safe and effective woundhealing agents, natural products from plant and mushroomsare currently being explored.

    Mushrooms have enormous potential as a source of bothdietary protein and health-enhancing dietary supplements[7]. Among the medicinal mushrooms, Ganoderma speciesare much sought after for their wide array of medicinalproperties. Ganoderma lucidum (M.A. Curtis:Fr.) P. Karstwhich belongs to the Polyporaceae family has long beenknown and used extensively in traditional Chinese medicine.In Malaysia, due to the high humidity and temperaturethroughout the year this mushroom is widely cultivated.Chemical analysis of the fruiting bodies of G. lucidumshowed the presence of polysaccharides and triterpenoids[810] that may have therapeutic values in the treatment orprevention of peripheral or central inflammatory diseases.Ganoderma spp. are a natural source of potent bioactiveantioxidant metabolites [11]. Total phenols were the majornaturally occurring antioxidant components found in bothG.lucidum and G. tsugae [12]. It has been reported that someplants, for example, Annona squamosa L. (Annonaceae),commonly known as the custard apple [13], as well as Catha-ranthus roseus L (Apocynaceae) flower [14], promotedwoundhealing in diabetic rats via free radical scavenging activityof flavonoids. To date, reports of the applications of themedicinal properties of mushrooms in the healing of woundsin diabetic rats are rather rare. Kwon et al. [15] reportedthat the cauliflower mushroom, Sparassis crispa Wulf.:Fr.(Aphyllophoromycetideae), improved the healing of diabeticwounds. The high -glucan (more than 40%) accounted forthe increase in the migration of macrophages and fibroblastsas well as elevated collagen synthesis. Sacchachitin and chitinmembrane prepared from the aqueous extracts of G. tsugaewere also found to have wound healing properties [16, 17].

    In our preliminary study using normal rats withoutdiabetes, the period of reepithelialisation and wound closureshowed no significant difference ( > 0.05) between theIntrasite-gel- and G. lucidum-treated groups (unpublisheddata). The effect of wound healing by the hot aqueous extractof G. lucidumwas comparable to that by Intrasite gel and thismight be due to its high content of polysaccharides (25.1%)and the synergistic reactions combining all the medicinalproperties as a whole. As wound healing in hyperglycemicstate is difficult and challenging, the aim of this study wasto further investigate the effect of the hot aqueous extract ofG. lucidum on wound healing and the oxidative damage instreptozotocin-induced diabetic rats.

    2. Materials and Methods

    2.1. Preparation and Standardisation of Hot Aqueous Extractof G. lucidum. The fresh fruiting bodies of G. lucidum wereobtained from Ganofarm Sendirian Berhad, a mushroomfarm in Tanjung Sepat, Selangor, Malaysia. The productionof G. lucidum was reported previously [18, 19]. The pow-dered fruiting bodies were subjected to hot water extraction

    (5 : 200, w/v) at 100C for 8 hours. The resulting aqueousextractwas freeze-dried and kept at20Cprior to use.Heavymetal composition and microbial load of selected pathogenswere analyzed by Nova Laboratory, Sepang, Malaysia, usingproprietary methodology. A voucher specimen ofG. lucidum(KLU-M 1233) was deposited in the herbarium ofMushroomResearch Centre, University of Malaya.

    2.2. Determination of Total Polysaccharides Content in HotAqueous Extracts of G. lucidum. The total polysaccharidecontent of the hot aqueous extract was determined usingthe phenol-sulphuric acid method with d-glucose as in [20].Briefly, one mL of 5% (w/v) phenol was added to one mL ofsample solution, followed by five mL of concentrated H


    The absorbance was measured using a spectrophotometer(Shimadzu series 1601UV/Vis) after 10 minutes at 483 nm.

    2.3. Quantification of Ganoderic Acid and Adenosine UsingHPLC. For determination of ganoderic acid A, Perkin ElmerSeries 200 liquid chromatography equipped with a PerkinElmer Series 200 UV detector was used. The detector signalwas recorded by the Turbochrom workstation software. Thecolumn was Hypersil BDS C18 (4.6 250mm) with Alltechrefillable C18 Guard column (10 4.6mm) (Alltech, USA).The mobile phase consisted of 5% (v/v) acetic acid inmethanol and the flow rate was 1.0mL/min. The calibrationcurve was prepared by injecting a series of ganoderic acidA (Sigma) reference standard dilutions. Quantification andvalidation of adenosine were also performed in Perkin ElmerSeries 200 liquid chromatography as mentioned. The mobilephasewasmethanol: 10mMmonobasic potassiumphosphate(15 : 85), pH 5.0, and the flow rate was 1.5mL/min. Bothganoderic acid A and adenosine were quantified by meansof calibration curves obtained from commercial standards ofthese compounds (Sigma).

    2.4. Determination of Cupric Reducing Antioxidant Capacity(CUPRAC) of Hot Aqueous Extract of G. lucidum. The cupricreducing antioxidant capacity (CUPRAC) of the hot aqueousextract of G. lucidum was determined according to themethod of Apak et al. [21]. Briefly, to a mixture of onemL of copper(II) (102M), neocuproine (7.5 103M), andammonium acetate buffer solution (1M), freshly-preparedmushroom extracts of varying concentrations were added tomake up a final volume of four mL. After incubation at roomtemperature (25 2C) for 30 minutes, the absorbance at450 nm was recorded against a reagent blank. The results ofantioxidant activity were expressed in


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