pôle phytoremediation

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Rencontres de l’ingénierie écologique en Bretagne Pôle Phytoremédiation 23 janvier 2013 – Carrefour des Gestions Locales de l’Eau Titre Contact Société / Organisme N°0 TREATMENT OF WASTEWATER BY PHYTOREMEDIATION IN ARID AREA Mohamed Mounir SAGGAÏ Laboratoire ECOBIO (CNRS, UR1, OSUR) 28 PHYTOREMEDIATION OF POLYCYCLIC AROMATIC HYDROCARBONS (PAHS) Anne-Sophie DUMAS Laboratoire ECOBIO (CNRS, UR1, OSUR) 29 STRESS RESPONSE TO POLYCYCLIC AROMATIC HYDROCARBON OF GLYCOPHYTES AND HALOPHYTES PLANT MODELS MOEZ SHIRI Laboratoire ECOBIO (CNRS, UR1, OSUR) 30

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Rencontres de l’ingénierie écologique en Bretagne

Pôle Phytoremédiation

23 janvier 2013 – Carrefour des Gestions Locales de l’Eau

Titre Contact Société / Organisme N°0

TREATMENT OF WASTEWATER BY PHYTOREMEDIATION IN ARID AREA Mohamed

Mounir SAGGAÏ

Laboratoire ECOBIO (CNRS, UR1, OSUR)

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PHYTOREMEDIATION OF POLYCYCLIC AROMATIC HYDROCARBONS (PAHS) Anne-Sophie DUMAS Laboratoire ECOBIO (CNRS, UR1, OSUR)

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STRESS RESPONSE TO POLYCYCLIC AROMATIC HYDROCARBON OF GLYCOPHYTES AND HALOPHYTES PLANT MODELS

MOEZ SHIRI Laboratoire ECOBIO (CNRS, UR1, OSUR)

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Treatment of wastewater by phytoremediation in arid area Mohamed Mounir SAGGAÏ1, Moez SHIRI2, Anne-Sophie DUMAS2, Ryad DJERIBI3 and Abdelhak EL AMRANI2

1 Ouargla University (Algeria) 2 Rennes1 University (France)/ ECOBIO Laboratory

3 Annaba University (Algeria)

Research topic

- Ecotoxicology

- Ecological restoration

- Phytoremediation

Activities and Expertise

Design, construction and control of Wet Areas Wastewater Treatment,

Coordonnées CNRS UMR 6553

6553 Ecobio: Ecosystème,Biodiversité, Evolution

Campus de Beaulieu, CS 742005

35042 Rennes Cedex, France

Mohamed Mounir SAGGAÏ/ Abdelhak El Amrani

[email protected]

[email protected]

Les Rencontres de l’ingénierie écologique en Bretagne, au 14e Carrefour des Gestions Locales de l’Eau, le 23 Janvier 2013 à Rennes

Captions

Abstract The aim of this study was the monitoring of the effectiveness of a system of sewage from domestic waste through the use of macrophytes in

an arid climate. The experimental system was designed as fellow; the duration of treatment was 07 days (Pic.1). The reactor effluent through the basin by gravity with a flow of 4 L/min. The retention time in the decanter was 60 hours. Irrigation frequency is weekly. The parameters studied are: (TSS, BOD5, COD, EC, pH), nutrients (total nitrogen: total phosphorus and TKN: TP) and microbiological parameters.

The results showed encouraging performance, decline in organic load and microbiologic load (TSS: 95.75%, BOD5: 91.15%, COD: 91.92%, PT: 24.90% pH 7.5).

Image 3

Pic.1

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Thematic topic The arid climate and vegetation gives specificity to Saharan regions, which

are characterized by a typical ecological fragility, this requires caution in approaches and choices to deal with environmental problems.

phytoremediation approach may be used to clean up several sources of pollution, the challenge of this project was to exploit the flora of these regions and their potential to serve this choice (Pic.1).

Problematic The project was led and determined to control the potential of a water treatment system by macrophytes (plants that grown in the study area), and study the behavior of Selected species without forgetting the quality of the water obtained after treatment (PIC. 2, 3 & 4) .

Subjects of study • Selected plant can reduce pollution levels to acceptable levels?

• The cleaning up capability is more or less stable throughout the year?

Our Analyzes allowed us to determine the potential of WWG system.

A new approach was developed with the laboratory of ECOBIO for the treatment of PAHs.

The species studied showed high capability to reduce the concentration of PAHs (Pic. 4)

These preliminary results open opportunities for broader uses of Saharan plants for phytoremediation.

The project partners are: - Ministry of Water Resources (Algeria)

- National Sanitation Office (ONA)

- Wastewater Garden® (WWG).

- APC Témacine

- University of Rennes 1

Macrophytes Phyto-purification Domestic wastewater Organic load Arid climate

Schematic design of the processing station by macrophytes

Pic. 2 Pic. 3 Pic. 4

Pic.1

Pic. 2

Pic. 3

Pic. 4

Input-output balance for BOD5 (Biological Demand of Oxygen )

Input-output balance for COD (Chemical Demand of Oxygen )

Variation of phenanthrene (enter, center and Exit)

Phytoremediation of polycyclic aromatic hydrocarbons (PAHs)

Anne-Sophie Dumas1, Richard Berthomé2, Moez Shiri1, Mohamed Mounir Saggaï1 and Abdelhak El Amrani1 1CNRS, Université de Rennes 1, UMR 6553, Ecosystèmes-Biodiversité-Evolution, 35042 Rennes cedex, France,

2URGV, UMR INRA 1165 - CNRS 8114 - UEVE, 2, Rue Gaston Cremieux, CP5708, 91057 Evry cedex, France.

Fields of Research o Decontamination of PAH-contaminated sites

o Green/ sustainable technologies

o Interface environmental stress-genome

UMR 6553 Ecobio- Université de Rennes 1- CNRS Campus de Beaulieu, Avenue du Général Leclerc 35042 RENNES Cedex – France http://ecobio.univ-rennes1.fr/news.php

Contact Anne-Sophie Dumas (PhD student) - Abdelhak El Amrani (Supervisor) +33 (0)2 23 23 58 26 [email protected]

Projects:

• In-vitro analysis of PAHs effects on model plant, Arabidopsis thaliana, to understand how plants cope with PAHs stress at different scales (Fig 3.) :

Plant development in PAH contaminated media

PAH effects on genome expression (1) and on metabolome at short-term (30 min to 24h)

PAH quantification in plants (2) and localization of PAH in plants.

Some additives, such as sucrose, enhance plant tolerance to some xenobiotics: is it appliable to PAHs?

• Design and monitoring of a full-scale field trial of PAH phytoremediation: comparison between several plant species, impact of bringing a uncontaminated soil with its micro-organisms and worms on the rates of PAH degradation (3)

Issues

o How to optimize in-situ phytoremediation of PAH?

o What are the processes of detoxification of PAHs in plants?

Studies o Create remediation systems combining plants, micro-organisms and

agricultural practices (amendments, tillage) that are adapted to the site, the type of contaminant

o Identify and optimize the mechanisms of plants PAH metabolism

o Use our knowledge on detoxification systems in plants and micro-organisms to enhance phytoremediation by creating or selecting plants species that are more tolerant and more efficient

Possible projects for the future…

• Testing other species and a mix of several species (trees and grass) on a PAH contaminated soil

• Create a guideline that will help in the set-up of a phytoremediation system Determination of the adapted species Establishing a timeline of treatment

Phytoremediation PAH Field trial Molecular mechanisms Plants Green technology

Activities o Design and monitoring of PAH phytoremediation sites

o Molecular biology, in-vitro culture, GC-MS analysis

o Transcriptomics , metabolomics

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Les Rencontres de l’ingénierie écologique en Bretagne 14e Carrefour des Gestions Locales de l’Eau,

23 Janvier 2013 - Rennes

Collaborations (1) URGV, INRA-CNRS, Evry, France (2) Géosciences, CNRS - University of Rennes 1, Rennes, France (3) Axson (formerly Bs Coatings), Aubevoye, France.

Stress response to polycyclic aromatic hydrocarbon of glycophytes and Halophytes plant models (Arabidopsis thaliana, Thellungiella salsuginea)

Moez Shiri1, Med Mounir Saggaï, Anne-Sophie Dumas, Chedly Abdelly1 and Abdelhak El Amrani1 1CNRS, Université de Rennes 1, UMR 6553, Ecosystèmes-Biodiversité-Evolution, équipe Mécanismes à l’Origine, LPE, laboratoire des plantes extrêmophiles

de la Biodiversité, 35042 Rennes cedex, France

Knowledge - skills studies of the mechanism of tolerance to abiotic stress.

Research project

o hydrocarbon pollution

o functional genomics of phytoremediation

o Bioremediation and extremophile plants

o Activities and Expertise

o treatment of contaminated sites by organic pollution and heavy metals

o Application of biological processes…

UMR 6553 Ecobio Avenue du Général Leclerc Campus de Beaulieu 35042 RENNES Cedex - France 33.(0)2.23.23.58.26

http://ecobio.univ-rennes1.fr

Moez Shiri/ Abdelhak El Amrani Campus Beaulieu, bât 14a, p.122 CS 74205 - 35042 RENNES CEDEX 02.23.23.58.26

[email protected]

[email protected]

Les Rencontres de l’ingénierie écologique en Bretagne, au 14e Carrefour des Gestions Locales de l’Eau, le 23 Janvier 2013 à Rennes

Response of plants to PAH stress

Project

This thesis project is a collaboration between Rennes university and the Laboratory of Biotechnology and Extremophiles Plant of Borj Cedria center. It will study the physiological, biochemical and molecular responses of plants to abiotic stress.

This study will focus on how plants may clean up contaminated environment.

Image 4

Image 1

Hypothesis Extremophile plants have significant potential in terms of

adaptation and tolerance. They have already proven their ability to grow in extreme conditions, and were used to desalinate and decontaminate soil polluted by heavy metal. These traits may help extremophile plants to clean up PAH-contaminated sites.

Issues o Identify the mechanisms of degradation and sequestration of

PAHs in extremophile plants.

o Identify genes involved in stress tolerance towards PAHs

o Isolate the gene or genes responsible for PAHs degradation and overexpress them in plant in order to increase the remedial competitiveness

Polycyclic aromatic hydrocarbon phytoremediation Oxidative stress Abiotic stress Glycophyte Halophyte

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