polarizing microscope optiphot - o avoid sharp knocks! handle the microscope gently, taking care to...

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  • Nikon

    Polarizing Microscope

    OPTIPHOT -POLINSTRUCTIONS

    NIPPON KOGAKU K. K.

  • CAUTIONS

    oAvoid sharp knocks!Handle the microscope gently, taking careto avoid sharp knocks.

    f}When carrying the microscopeWhen carrying the microscope, hold its armwith one hand, supporting the bottom ofthe microscope base with the other. Theinstrument weighs about 10.5 kg. Do nothave the lamp housing carry any load.

    8Place for usingAvoid the use of the microscope in a dustyplace, where it is subject to vibrations orexposed to high temperatures, moisture ordirect sunlight.

    oPower source voltageIn every case, make sure of the powersource voltage by means of the inputvoltage change-over switch on the bottomof the microscope base.

    oLight sourceHalogen lamp bulb to be used is 12V-50W. Do not use 12V-l OOWhalogen lampbulb. If the lamp bulb of over-rated wat-tage is used, light adjusting circuit willdamage.Never connect the lamp housing cord tothe house current socket directly.

    o In lighting the lampTake care not to touch the lamp housingbeing lighted, and don't bring inflammablesubstances such as gasoline, thinner, andalcohol near to the lamp housing, as someparts of the lamp housing may take a hightemperature while the lamp is being light-ed.

    oExchanging the lamp bulb and fuseBefore replacing the lamp bulb or fuse,turn 0 FF the power switch and disconnectthe plug of the power source cord.In such cases as of replacement, do nottouch the lamp bulb with bare hands, im-med iately after putting out the lamp.

    ;) Dirt on the lensDo not leave dust, dirt or finger marks onthe lens surfaces.They will prevent you from clear observa-tion of the specimen image.

    oStrain-free glassesThe optical elements of this microscopebeing constructed of strain-free glasses,take particular caution in handling theobjectives and condenser lenses not tocause strain to them.

    4D> Focus knobsNever attempt to adjust the tightness ofthe right- and lefthand focus knobs byturning the one, while holding the other inth is model microscope, because of causi ngdisorder.

  • CARE AND MAINTENANCE CONTENTS

    0....... 08

    NOMENCLATURE

    II. ASSEMBLY.

    I.

    III. PREPARATION1. Centering the Lamp 82. Interpupillary Distance Adjustment 03. Diopter Adjustment 04. Optical Path Change-over in the

    Trinocular Eyepiece Tube "TP" 05. Centering the Objectives 06. Centering the Condenser Lens 4@

    IV. MICROSCOPY 411. Operating Procedure 412. Manipulation of Each Element ~

    1) Focusing ~2) Condenser aperture diaphragm ~3) Field diaphragm ~4) Circular graduated stage ~5) Objectives 4J)6) Eyepieces 4J)7) Achromat strain-free condenser 4J)8) Bertrand lens 4D9) 1/4 A & tint plate 4D10) Dia-polarizer and analyzer 4D11) Filters G)12) Lowering the substage G)13) Illumination system G)

    V. PHOTOMiCROGRAPHy 4D

    VI. ACCESSORIES ~1. Senarmont Compensator ~2. Quartz Wedge ~3. Monocular Eyepiece Tube "AP" W4. Universal epi-illuminator W5. Attachable Mechanical Stage Type "E". ~6. Universal Stage ~

    VII. TROUBLE SHOOTING TABLE ~

    REFERENCE ~

    ELECTRIC SPECIFICATIONS 0

    8Cleaning the painted surfacesAvoid the use of any organic solvent (forexample, thinner, ether, alcohol, xyleneetc.) for cleaning the painted surfaces andplastic parts of the instrument.

    oCleaning the lensesTo clean the lens surfaces, remove dustusing a soft brush or gauze. Only forremoving finger marks or grease, shouldsoft cotton cloth, lens tissue or gauzelightly moistened with absolute alcohol(ethanol or methanol) be used.For cleaning the objectives and immersionoil use only xylene.Observe sufficient caution in handlingalcohol and xylene.

    8Never attempt to dismantle!Never attempt to dismantle the instrumentso as to avoid the possibility of impairingthe operational efficiency and accuracy.

    oWhen not in useWhen not in use, cover the instrument withthe accessory vinyl cover, and store it in aplace free from moisture and fungus.It is especially recommended that theobjectives and eyepieces be kept in anairtight container containing desiccant.

    oPeriodical checkingTo maintain the performance of the instru-ment, we recommend to check the instru-ment periodically. (For details of thischeck, contact our agency.)

  • I .NOMENCLATURE

    Interpupillary distance scale

    Diopter ring

    CF eyepiece

    Eyeguard

    Bertrand lens ring

    Centering nosepiece

    CF Achromat P objective(Stra in-free)

    Vernier

    Circular graduated stage

    Achromat strain-free condenser

    Orientation plate

    Field lens

    Brightness control dial(including power switch)

    Microflex clamp screw

    Trinocular eyepiece tube "TP"

    Optical path change-over knob

    Eyepiece tube clamp screw

    Analyzer knob-----------I Intermediate tube clamp screw111'1,

    Substage clamp screw

    Socket sleeve

    Lamp verticalcentering ring

    Lamp lateralcentering screw

    Lamp input plug

    Filter receptacle

    Filter frame

    ~ ND16&GIFI(NCB10. ND2.

    Dust capLamp centering tool

    Fig. 1

  • Analyzer rotation ring

    Analyzer clamp screw

    Intermediate tube "P"

    Nosepiece clamp screw

    45 click stop lever

    Condenser focus knob

    Coarse focus knob

    Fine focus knob

    Lamp housing

    Lamp housingclamp screw

    Arm rest

    -..

    Compensator slot

    1/4 A & tint plate

    Nosepiece centering screw

    Specimen clip

    Condenser aperturediaphragm control ring

    Dia-polarizer-------Condenser clamp screw

    Brightness indicator

    Field diaphragm control ring

    X-POL stand

    Fig.2

  • II. ASSEMBLY To assemble the microscope, follow the procedure in the order given:

    Substageclamp scre,

    Achromat strain-free condenserInsert the condenser into the con-denser carrier, facing the aperturenumber plate toward the operator.Fasten the clamp screw on the leftside of the carrier.

    Circular graduated stageRelease the substage clamp screw usinga driver, and sl ide the substage on thedovetail fitting. In such a position thatthe top ends of the substage and thedovetail are at the same level, fastenthe clamp screw.

    CF eyepieceInsert the eyepiece CFW 10XCMinto the right-hand sleeve, fittingthe pin of the eyepiece in theright-hand notch of the sleeve.Into the left-hand sleeve, inserttheCFW10X.

    1/4 A & tint plateRemove the screw by the side ofthe 1/4 "A. plate of the 1/4 "A. & tintplate and insert it into the com-pensator slot of the intermediatetube "P", facing the positioninggroove toward the operator side.Reattach the removed screw.

    Specimen clipPlace the clip on the stageusing holes on the stage sur-face.

    @

    Aperturenumberplate

    @ Centering nosepiece17 Lower the stage sufficiently.Releasing the nosepiece clampscrew on the left side of themicroscope arm, insert the nose-piece. Making sure of positivefitting of the pin on the micro-scope arm into the nosepiecegroove, refasten the clamp screw.

    @

    @6CF Achromat P objective (Strain-free)Mount the objectives on the nose-piece in such positions that theirmagnifying power increases as thenosepiece is revolved clockwise.

    @

    lowest power

    Leveling foot screwFor stable installation of themicroscope, manipulate theadjusting screw at one foot onthe bottom of the microscopebase.

    Power source voltageSet the input voltage to the powersource voltage by means of thechange-over switch on the bottomof the base.

    If the illumination is found toobright or unstable, when the switch isturned ON, make adjustment of thelowest voltage in the following way:1) Turn the brightness control dialto OFF.

    2) Turn the lowest voltage adjustingscrew on the bottom of themicroscope base counterclockwiseto the limit, using a screw driver.

    3) Turn the brightness control dialto ON_ At this time, the lampvoltage will be highest, immedi-ately after lighting.

    4) In this condition, gently turn thelowest voltage adjustingsc;:ewclockwise, to set the voltage tonearly 4 on the ind icator.

    Bottom of the Base

    Bottom of the Base~ -------~

    Bottom of the Base

  • Eyepiece tubeAttach the eyepiece tube on the intermediate tube "P", fittingthe notch of the circular dovetail on the end of the clamp screw.Fasten the clamp screw.

    Intermed iate tube "P"Attach the intermediate tube "P" on the arm of the X-POL stand,fitting the notch of the circular dovetail on the end of theclamp screw. Fasten the clamp screw.

    Lamp input plugConnect the plug to thereceptacle on the filterreceptacle's left side.

    Lamp housingInsert the lamphousing into the col-lector lens, and fas-ten the clamp screwon the left side ofthe housing.

    @ FilterPlace the filter into the filterframe. After centering the lamp,put the filter with the frame intothe filter receptacle. No frame isprovided for the diffuser.

    Power input plugConnect the plug to the re-ceptacle on the fi Iter recep-tacle's right side.

    Insert the lamp bulb withits pins into the acceptingholes in the socket.Note: Don't touch the

    bulb surface directlywith the fi nger.

    f3\ Halogen lamp bulb~ (12V-50W)

    Filter frame

    @

    @

    Eyepiece tube clamp screw

    Socket sleeveInsert the socket sleeve intothe lamp housing, and fastenit firmly with the clamp screw.

    Socket sleeve clamp screw

    Fig. 3

  • III. PREPARATION

    Lamp housingclamp screw

    Filamentimage

    Condenseraperturediaphragm

    Socket sleeveclamp screwLamp verticalcentering ring

    Lamp lateralcentering screw

    /

    Fig. 6

    7) Release the socket sleeve clamp screw (Fig.6). Turning the lamp lateral centering screwand vertical centering ring, bring the fila-ment image to the center,

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