platelet function testing

28
Platelet Function Testing Kit for each patient The packet contains everything needed We will now demonstrate how to perform the test

Upload: rufin

Post on 07-Jan-2016

83 views

Category:

Documents


2 download

DESCRIPTION

Platelet Function Testing. Kit for each patient The packet contains everything needed. We will now demonstrate how to perform the test. Three platelet stimulant tubes. Instruction leaflet Citrate blood collection tube. aspirin test clopidogrel test baseline test. insulated pouch - PowerPoint PPT Presentation

TRANSCRIPT

Page 1: Platelet Function Testing

Platelet Function Testing

Kit for each patient

The packet contains everything needed

We will now demonstrate how to perform the test

Page 2: Platelet Function Testing

insulated pouchheating padconnecting 2way adaptorsfixing solution

aspirin testclopidogrel testbaseline test

Instruction leafletCitrate blood collection tube

Three platelet stimulant tubes

Page 3: Platelet Function Testing

Activate the heating pad by clicking on the circular valve inside

Warming the tubes

Page 4: Platelet Function Testing

When activated the gel turns white and warms up immediately

Warming the tubes

Page 5: Platelet Function Testing

Place the heated pad inside one pocket of the insulation pouch

Warming the tubes

Page 6: Platelet Function Testing

Place the three stimulant tubes in the other pocket of the insulation pouch

Warming the tubes

Page 7: Platelet Function Testing

The stimulant tubes are now ready to use

Warming the tubes

Page 8: Platelet Function Testing

Venepuncture

Select the green topped citrate monovette

Blood should preferably be collected using one of the following venepuncture procedures

ButterflyBD VacutainerSarstedt Multifly set

Page 9: Platelet Function Testing

Butterfly you will also need the Sarstedt multi-adaptor valve

Venepuncture

Page 10: Platelet Function Testing

BD Vacutainer Safety-Lok you also need the Sarstedt multi-adaptor valve

Venepuncture

Page 11: Platelet Function Testing

the Sarstedt multi-adaptor valve

Venepuncture

Page 12: Platelet Function Testing

Sarstedt MultifySet already supplied with the adaptor for the monovette tube

Venepuncture

Page 13: Platelet Function Testing

Filled citrate anticoagulant tube

Fill to the 10 ml line by pulling gently on the plunger (do not lock off the tube)Invert 3 times to mix the blood

Page 14: Platelet Function Testing

Pull the plunger pulled back (do not lock off the tube)Add the blood from the syringe up to the 10 ml markScrew on the green capInvert 3 times to mix the blood

If you have to use a needle and syringe

If using a needle and syringe the tube will need to be opened by holding the monovette upright and unscrewing the green end taking care not to spill the anticoagulant inside.

10 ml mark

Venepuncture

Page 15: Platelet Function Testing

Filled citrate anticoagulant tube

Page 16: Platelet Function Testing

Place the blood in the insulation pouch with the stimulant tubesuntil ready to perform the test

Page 17: Platelet Function Testing

Mix the blood and attach one of the 2way adaptors

Platelet stimulation

Page 18: Platelet Function Testing

Attach each stimulant tube and add blood in turn

Fill three stimulant tubes

Platelet stimulation

Page 19: Platelet Function Testing

To fill, pull back plunger until top of the plunger aligns with bottom arrow

Blood should flow up to4.5 ml mark

Platelet stimulation

Page 20: Platelet Function Testing

Place the stimulant tubes back in the pouch to keep warm

Check the time and wait 5 minutes!

Platelet stimulation

Page 21: Platelet Function Testing

While waiting for the 5 minutes

Discard the used citrate tube and adaptor valve

Page 22: Platelet Function Testing

While waiting for 5 minutes

Take the second 2way adaptor and attach to the Fixing Solution Tube

Page 23: Platelet Function Testing

When 5 minutes is passed

Attach each stimulant tube in turn to the 2way adaptor Pull back plunger all the way and fill the tubes with the fixing solution (liquid aligns with the 4.5 ml mark)

Page 24: Platelet Function Testing

Completed stimulant tubes

Invert to mix

Page 25: Platelet Function Testing

Snap off the plungers on the stimulant tubes

Page 26: Platelet Function Testing

Write patient details on the labels on each of the three stimulant tubes

Page 27: Platelet Function Testing

Place the completed stimulant tubes in the padding for transportation

Page 28: Platelet Function Testing

Place tubes inside the packageand transfer pack to Nottingham

Platelet functionwill be measured asplatelet P-selectin expression