pi = (pk 1 + pk 2 )/2, the ph where the molecule has a net charge of 0
DESCRIPTION
pI = (pk 1 + pk 2 )/2, the pH where the molecule has a net charge of 0. Ninhydrin Test. Ion Exchange Chromatography. Step 1. Separation of Polypeptide Chains. dissociated by exposure to pH extremes, 8 M urea, 6 M guanidinium hydrochloride, or high salt cencentrations. - PowerPoint PPT PresentationTRANSCRIPT
Phe-Ser-Tyr-Gly-Val-Gln
64 69
O2N
N
NO
HH
Phe
O
Val
OGln
HO
blue
green
green fluorescent protein (GFP)
Protein separation
size exclusion chromatography
ultrafiltration
ultracentrifugation
least soluble at their IP
Step 1. Separation of Polypeptide Chains
dissociated by exposure to pH extremes, 8 M urea, 6 M guanidinium hydrochloride, or high salt cencentrations
Edman degradation
phenylisothiocyanate
50 cycles of reaction can be accomplished on 50 pmol (about 0.1 g)
Fmoc solid phase peptide synthesis: A Practical Approach
Edited by
Weng C. Chan and Peter D. White
OXFORD UNIVERSITYPRESS
2000