Photos in Living ColorPhotos in Living Color

Purdue University iGEM TeamPurdue University iGEM Team

Purdue Purdue University - iGEMUniversity - iGEM

Team Advisor : Jenna L. Rickus, Ph.D.Team Advisor : Jenna L. Rickus, Ph.D.Assistant Professor- Dept. of Agricultural & Biological Assistant Professor- Dept. of Agricultural & Biological

Engineering & Weldon School of Biomedical EngineeringEngineering & Weldon School of Biomedical Engineering

Project Leader: John Schumm Project Leader: John Schumm Junior in Agricultural and Biological EngineeringJunior in Agricultural and Biological Engineering

Graduate Supervisor: John KoehlerGraduate Supervisor: John KoehlerMaster’s in Agricultural & Biological EngineeringMaster’s in Agricultural & Biological Engineering

Aaron YoungAaron YoungSophomore in Biomedical EngineeringSophomore in Biomedical Engineering

Piyush BajajPiyush BajajSenior in Biomedical EngineeringSenior in Biomedical Engineering

AcknowledgementsAcknowledgements

Agricultural and Biological Agricultural and Biological EngineeringEngineering

Purdue Resources in EngineeringPurdue Resources in Engineering Prof. Jenna RickusProf. Jenna Rickus Prof. Kari ClaseProf. Kari Clase Prof. Nathan MosierProf. Nathan Mosier Bindley Bioscience CenterBindley Bioscience Center Purdue Laboratory of Renewable Purdue Laboratory of Renewable

Resources in EngineeringResources in Engineering

Purdue UniversityPurdue University HistoryHistory

Founded in 1869 and named Founded in 1869 and named after benefactor John Purdue, after benefactor John Purdue, Purdue University began its Purdue University began its journey with six instructors, journey with six instructors, 39 students and a mission to 39 students and a mission to provide agriculture and provide agriculture and mechanic arts education.mechanic arts education.

Student BodyStudent BodyWest Lafayette enrollment of West Lafayette enrollment of 38,712 students (fall 2005); 38,712 students (fall 2005); students from 50 states and students from 50 states and 130 countries.130 countries.

Purdue University enjoys Purdue University enjoys global renown for its world-global renown for its world-class instructional and class instructional and research pursuits.research pursuits.

iGEM & PurdueiGEM & Purdue As one of the largest As one of the largest

universities in the universities in the nation, Purdue is nation, Purdue is acclaimed for the quality acclaimed for the quality of its teaching and of its teaching and research in a wide range research in a wide range of fields.of fields.

Participating for the first Participating for the first time in the iGEM time in the iGEM competition.competition.

Formation of a student Formation of a student organization titled organization titled Biological Engineering in Biological Engineering in Genetics.Genetics.

In the Beginning…In the Beginning…

BEG is a completely student run BEG is a completely student run organization and an officially recognized organization and an officially recognized Purdue club.Purdue club.

To gain such status, a constitution was To gain such status, a constitution was written to govern the club.written to govern the club. This included officer elections, lab safety, and This included officer elections, lab safety, and

other foreseeable issues.other foreseeable issues. Completed and submitted to Purdue for approval Completed and submitted to Purdue for approval

and status.and status. Makes Purdue BEG unique and appealing to Makes Purdue BEG unique and appealing to

donors as it is student run and directed.donors as it is student run and directed.

Did Somebody say Did Somebody say Money?Money?

Fundraising conducted within school Fundraising conducted within school departments to pay for research, travel, and departments to pay for research, travel, and other associated costs.other associated costs.

Packet of information developed and Packet of information developed and distributed during sales pitch.distributed during sales pitch. Samples of used material will be available on the Samples of used material will be available on the

BEG website for future reference by other schools.BEG website for future reference by other schools. Referencing student basis, students have Referencing student basis, students have

control of funds but cannot create control of funds but cannot create expenditures without approval of Advisor and expenditures without approval of Advisor and Treasurer.Treasurer.

The IdeaThe Idea Fluorescent Fluorescent

Biological Photo Biological Photo PaperPaper

Use ink Jet printer to apply repressors/inducers to sheet of paper.

Incubate Paper with Genetically Modified e-coli that express fluorescent colors in presence of these repressors/inducers.

Part PrioritiesPart Priorities

Use water soluble molecules.Use water soluble molecules. IPTG and tetR IPTG and tetR

Use an antibiotic to kill e-coli in Use an antibiotic to kill e-coli in white space.white space. KanamycinKanamycin

Signal for strong colored fluorescent Signal for strong colored fluorescent proteinsproteins GFP, mRFPGFP, mRFP

Part SelectionPart Selection

Three Parts Three Parts usedused GFP GFP

BBa_l13521BBa_l13521 RFP RFP

BBa_j04430BBa_j04430 tetR PlasmidtetR Plasmid

Part Creation- Standard Part Creation- Standard AssemblyAssembly

The ProcessThe Process

Prepared Competent CellsPrepared Competent Cells Transformation of DNATransformation of DNA MiniprepMiniprep Enzyme DigestEnzyme Digest Gel ElectrophoresisGel Electrophoresis Gel ExtractionGel Extraction LigationLigation RepeatRepeat

Printer ProblemsPrinter Problems Loading Loading

ProblemsProblems Ink/ Promoter Ink/ Promoter

ViscosityViscosity Diffusion Diffusion

throughout the throughout the paperpaperPrinter Solutions

•Find an easily refillable printer

•Print trial with table sugar

Biological Biological ProblemsProblems

Bacteria Bacteria Growing on Growing on PaperPaper Will it stay in one Will it stay in one

place?place? Diffusion of Diffusion of

Promoters in Promoters in mediamedia

Resolution of Resolution of GFP mRFPGFP mRFP

Future PlansFuture Plans Recruit full time Recruit full time

student research student research over the summerover the summer 3-5 Students3-5 Students Use the Purdue Use the Purdue

SURF programSURF program Expand into other Expand into other

engineering engineering disciplinesdisciplines

iGEM plansiGEM plans

Keep the project Keep the project student run student run Allow the next Allow the next

generation of generation of students to pick their students to pick their projectproject

Encourage members Encourage members with little experience with little experience to jointo join

Obtain dedicated lab Obtain dedicated lab space and space and equipmentequipment

FundingFunding

Need a reliable source of annual Need a reliable source of annual fundingfunding Lab equipment, trips, summer studentsLab equipment, trips, summer students Goal is an endowment through Goal is an endowment through

Discovery ParkDiscovery Park

Questions Questions