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Abstracts / Toxicology L

olice, a cup with green liquid and a bowel with the remnants ofhe cooked plants were found.

The patient was admitted to the Clinic of Toxicology. In addi-ion to the liquid and cooked plants, biologic material was sent tohe lab too (blood, urine, stomach contents). Toxicology analyses ofhe samples was performed by the GC/MS method. The presencef carbamazepine and midazolam was verified. Identification waserformed by the comparison of spectra of the samples with theass spectra library NIST 90. Quantification was done using the

nternal standard method. Midazolam concentration in the bloodample was 0.40 �g/mL and carbamazepine 1.72 �g/mL.

Criminal poisoning is an excellent resource for forensic toxi-ologist. Antipsychotics and antidepressants are some of the mostrequently encountered drugs in forensic and clinical cases. Foregal-medical expertise, GC/MS is the method of choice for deter-

ination of drugs in the toxicological analysis.

oi:10.1016/j.toxlet.2009.06.800

19quick LC/MS/MS method for the analysis of common benzodi-

zepines and opiates

ania Sasaki 1,∗, Sumandeep Rana 2, Wayne Ross 2

Applied Biosystems, Foster City, United States, 2 Redwoodoxicology, Santa Rosa, United States

bjective: The objective of this paper is to develop a fast method fornalysis of common opiates and benzodiazepines in urine.

Methods: Analytes included in this method are: 6-onoacetyl Morphine (6-MAM), Codeine, Morphine, Oxycodone,ydrocodone, Hydromorphone, Desalkylflurazepam, Alprazo-

am, �-Hydroxyalprazolam, Diazepam, Nordiazepam, Lorazepam,xazepam, Temazepam, Triazolam, 7-Aminoclonazepam andlonazepam. Deuterated analogs of each analyte were used as

nternal standards.Urine samples were hydrolyzed, centrifuged for 2 min and

iluted 1:5 with LC mobile phase. LC/MS/MS analysis was per-ormed on a Shimadzu Prominence LC stack interfaced to anpplied Biosystems hybrid triple quadrupole/linear ion trapass spectrometer. Injection-to-injection analytical run time was

.5 min. Two MRM transitions per analyte were monitored and oneransition per internal standard. The Scheduled MRMTM algorithmas used for optimal method performance for this multi-analyteethod.Results: Results showed that all analytes were successfully

etected in the 6.5 min run time utilized. The LLOQs forost analytes was around ≤5 ng/mL and all analytes had an

LOQ ≤ 50 ng/mL. Precision and accuracy were both within 10%xcept at or near the LLOQ, where both precision and accuracy wereithin 15%. The linear dynamic range was at least three orders ofagnitude for all analytes.Conclusion: An LC/MS/MS method was developed to quickly

nalyze common benzodiazepines and opiates in urine. The min-mal sample preparation, combined with short LC/MS/MS runime drastically decreased sample turnaround time and increased

hroughput without compromising sensitivity or selectivity. Addi-ionally, the ability to combine two assays into one quick LC/MS/MSun further decreased analysis times and costs.

oi:10.1016/j.toxlet.2009.06.801

S

Dscpp

189S (2009) S57–S273 S255

20harmacoepidemiologic investigation regarding the interac-ions between antihypertensive medication and alcohol

iliana Tartau 1,∗, Elena Teslariu 2, Odetta Duma 3

University Med Pharm Gr.T.Popa, Pharmacology and Algesiology,asi, Romania, 2 University Med Pharm Gr.T.Popa, Occupational

edicine, Iasi, Romania, 3 University Med Pharm Gr.T.Popa, Publicealth and Sanitary Management, Iasi, Romania

ost of adult population consumes alcohol at least occasionally.he vast majority of hypertensive patients is treated with antihy-ertensive drugs for many years, and one important aspect may beepresented by the negative impact of alcohol on the effectivenessf this medications.

Aim: Pharmacoepidemiologic study regarding the interactionsetween antihypertensive medication and alcohol.

Method: Our investigation was performed during 6 monthn 148 hypertensive patients, from a primary medical centern Iasi. The study consists of medical sheets analysis, aboutypertension diagnose, daily medication recommended. Socio-emographic status, self-reported lifestyle and alcohol intake,esults of Cage test application, were obtained by using a ques-ionnaire. Data were statistically processed using t test from Excelrogram.

Results: In summary this study shows that all investigated hyper-ensive patients, received one or more antihypertensive drugs,n the situation of self-reported daily or occasionally alcoholntake. Of the 148 subjects who reported drinking alcohol over theast month, 83 (56%) reported alcohol intake within an hour, 2416%) drinking alcohol at the same time of taking antihyperten-ive drugs and 41 (28%) chronic alcohol consumption. Ingestionf alcohol caused a marked blood pressure reduction in hyper-ensive patients treated especially with �1-blockers (prazosin,oxazosin).

Conclusions: An important aspect is that, most of the inves-igated patients continued to consume alcohol at higher thanxpected levels, while also taking antihypertensive drugs. The inter-ctions between antihypertensive drugs and alcohol determine thencrease of adverse effects of this medication. In addicted persons, its absolutely necessary the downward titration of antihypertensiveose.

oi:10.1016/j.toxlet.2009.06.802

21ffect of diltiazem on retention and retrieval of memory inoung and aged mice

ahnaz Mehrabizadeh Honarmand 1,∗, Ardeshir Arzi 2, Shahrzadamshidi 2, Mahmoud Latifi 3

Ahvaz Shahid Chamran University, Psychology, Ahvaz, Islamicepublic of Iran, 2 Ahvaz Jondishapour University of Medical Sciences,epartment of Pharmacology & Toxicology, Pharmacy School, Ahvaz,

slamic Republic of Iran, 3 Ahvaz Jondishapour University of Medicalciences, Hygiene School, Ahvaz, Islamic Republic of Iran

iltiazem (DTZ) is widely used in the prophylaxis of hyperten-

ion and treatment of angina. The effects of DTZ and other calciumhannel blockers on memory have been discussed with variformrocedures and different theories have been suggested. In theresent study, the effect of DTZ on retention and retrieval of