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POSTER PRESENTATION Open Access Performance and body composition effects of a pre-workout supplement and post-workout protein intake in trained crossfit individuals Stacie Urbina 1* , Sara Hayward 1 , Jordan Outlaw 1 , Josh Holt 1 , Bailey Burks 1 , Brooke Cox 1 , Eliza Faillace 1 , Brittany Stai 1 , Matthew Stone 1 , Rob Wildman 2 , Shawn Wells 2 , Kristen Dunsmore 2 , Abbie Smith-Ryan 3 , Lem Taylor 1 , Cliffa Foster 1 , Colin Wilborn 1 From International Society of Sports Nutrition: 10th Annual ISSN Conference and Expo Colorado Springs, CO, USA. 14-15 June 2013 Background Despite widespread use of nutrition supplement s by CrossFit participants, existing data regarding perfor- mance and safety are minimal. Furthermore, increasing restrictions and drug testing in CrossFit, warrant the need for product specific research. The purpose of this study was to test the effects of a pre-workout supple- ment and post-workout protein & carbohydrate shake on CrossFit-specific performance measures and body composition. Methods In an open label randomized study, 11 males and 13 females (n=24, mean ± SD; 32.71 ± 7.39 yrs, 173.15 ± 11.54 cm, 76.83 ± 15.77kg, 22.00 ± 9.73% body fat) who were regular CrossFit participants (6 months), and not currently taking ergogenic supplements, completed the study. Subjects were tested at baseline (T1) and 6 weeks (T2). Body composition variables including lean muscle mass (LBM), fat mass (FM), and percent body fat (BF) were assessed using DEXA (Hologic Wi). Performance variables: cardiorespiratory fitness (VO 2 max), Wingate peak power (PP), and mean power (MP) were tested 24-48 hours after completing two Workouts of the Day (WOD) with 20 minutes rest in between (WOD1: 500m row, 40 wall balls, 30 push-ups, 20 box jumps, 10 thrus- ters for time; WOD2: 800m run buy in, followed by 15- minutes as many rounds as possible of 5 burpees, 10 Kettlebell swings, 15 air squats) at T1 and T2. Subjects were matched based on sex and number of days they participate in CrossFit workouts per week, and then ran- domly assigned to the supplement (SUP) or control (CTL) group. SUP consisted of 19g of a pre-workout drink (pomegranate fruit extract, beet root extract, tart cherry extract, AssuriTEAgreen tea, and InnovaTEAblack tea extract) 30 minutes before each CrossFit workout and post-workout supplement consisting of protein (40g for females and 80g for males) and carbohydrate (80g for females and 160g for males). CTL subjects did not con- sume anything one hour before or after each workout. Participants were required to complete at least three work- outs per week at a CrossFit gym, consume their supple- ment as directed, and complete mood state (MS), rate of perceived exertion (RPE), and delayed onset muscle sore- ness (DOMS) questionnaires. Data was analyzed by a group (SUP vs. CTL) × time (T1 vs. T2) repeated mea- sures ANOVA (p <0 .05). Results All data is presented as mean change scores. There were no time × group interactions for LBM (SUP 1130.86 ± 606.25 g; CTL 407.99 ± 728.42 g; p=0.36), FM (SUP 500.34 ± 437.82 g; CTL 107.77 ± 310.69 g; p=0.34) or BF (SUP 0.30 ± 0.21 %; CTL 0.06 ± 0.53 %; p=0.62). However, there was a significant trend for LBM (p = 0.063). There was no significant change in performance for VO 2 max (SUP -0.43 ± 1.88 ml/kg/min; CTL -1.525 ± 1 ml/kg/min; p=0.13), MP (SUP 6.54 ± 3.06 W; CTL 5.92 ± 2.91 W; p=0.58) or PP (SUP -8.76 ± 25.44 W; CTL 26.09 ± 21.74 W; p=0.54). Though there was no 1 University of Mary Hardin-Baylor, Human Performance Lab, 900 College Street, Belton, TX 76531, USA Full list of author information is available at the end of the article Urbina et al. Journal of the International Society of Sports Nutrition 2013, 10(Suppl 1):P28 http://www.jissn.com/content/10/S1/P28 © 2013 Urbina et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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POSTER PRESENTATION Open Access

Performance and body composition effects of apre-workout supplement and post-workoutprotein intake in trained crossfit individualsStacie Urbina1*, Sara Hayward1, Jordan Outlaw1, Josh Holt1, Bailey Burks1, Brooke Cox1, Eliza Faillace1, Brittany Stai1,Matthew Stone1, Rob Wildman2, Shawn Wells2, Kristen Dunsmore2, Abbie Smith-Ryan3, Lem Taylor1, Cliffa Foster1,Colin Wilborn1

From International Society of Sports Nutrition: 10th Annual ISSN Conference and ExpoColorado Springs, CO, USA. 14-15 June 2013

BackgroundDespite widespread use of nutrition supplement s byCrossFit participants, existing data regarding perfor-mance and safety are minimal. Furthermore, increasingrestrictions and drug testing in CrossFit, warrant theneed for product specific research. The purpose of thisstudy was to test the effects of a pre-workout supple-ment and post-workout protein & carbohydrate shakeon CrossFit-specific performance measures and bodycomposition.

MethodsIn an open label randomized study, 11 males and 13females (n=24, mean ± SD; 32.71 ± 7.39 yrs, 173.15 ±11.54 cm, 76.83 ± 15.77kg, 22.00 ± 9.73% body fat) whowere regular CrossFit participants (≥6 months), and notcurrently taking ergogenic supplements, completed thestudy. Subjects were tested at baseline (T1) and 6 weeks(T2). Body composition variables including lean musclemass (LBM), fat mass (FM), and percent body fat (BF)were assessed using DEXA (Hologic Wi). Performancevariables: cardiorespiratory fitness (VO2max), Wingatepeak power (PP), and mean power (MP) were tested24-48 hours after completing two Workouts of the Day(WOD) with 20 minutes rest in between (WOD1: 500mrow, 40 wall balls, 30 push-ups, 20 box jumps, 10 thrus-ters for time; WOD2: 800m run buy in, followed by 15-minutes as many rounds as possible of 5 burpees, 10Kettlebell swings, 15 air squats) at T1 and T2. Subjects

were matched based on sex and number of days theyparticipate in CrossFit workouts per week, and then ran-domly assigned to the supplement (SUP) or control (CTL)group. SUP consisted of 19g of a pre-workout drink(pomegranate fruit extract, beet root extract, tart cherryextract, AssuriTEA™ green tea, and InnovaTEA™ blacktea extract) 30 minutes before each CrossFit workout andpost-workout supplement consisting of protein (40gfor females and 80g for males) and carbohydrate (80gfor females and 160g for males). CTL subjects did not con-sume anything one hour before or after each workout.Participants were required to complete at least three work-outs per week at a CrossFit gym, consume their supple-ment as directed, and complete mood state (MS), rate ofperceived exertion (RPE), and delayed onset muscle sore-ness (DOMS) questionnaires. Data was analyzed by agroup (SUP vs. CTL) × time (T1 vs. T2) repeated mea-sures ANOVA (p <0 .05).

ResultsAll data is presented as mean change scores. There wereno time × group interactions for LBM (SUP 1130.86 ±606.25 g; CTL 407.99 ± 728.42 g; p=0.36), FM (SUP500.34 ± 437.82 g; CTL 107.77 ± 310.69 g; p=0.34) orBF (SUP 0.30 ± 0.21 %; CTL 0.06 ± 0.53 %; p=0.62).However, there was a significant trend for LBM (p =0.063). There was no significant change in performancefor VO2max (SUP -0.43 ± 1.88 ml/kg/min; CTL -1.525± 1 ml/kg/min; p=0.13), MP (SUP 6.54 ± 3.06 W; CTL5.92 ± 2.91 W; p=0.58) or PP (SUP -8.76 ± 25.44 W;CTL 26.09 ± 21.74 W; p=0.54). Though there was no

1University of Mary Hardin-Baylor, Human Performance Lab, 900 CollegeStreet, Belton, TX 76531, USAFull list of author information is available at the end of the article

Urbina et al. Journal of the International Society of Sports Nutrition 2013, 10(Suppl 1):P28http://www.jissn.com/content/10/S1/P28

© 2013 Urbina et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative CommonsAttribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction inany medium, provided the original work is properly cited.

significant group × time interaction for WOD2 (SUP17.08 ± 7.25 reps; 9.07% increase; CTL 4.91 ± 14.07 reps;2.46% increase; p=0.23), there was a significant maineffect for time (p=.037). A significant group × time inter-action for WOD1 was observed (p =0.05; SUP -58.33 ±52.31 seconds; 10.43% decrease; CTL -3.66 ± 14.38seconds; 0.73% decrease).

ConclusionThe combination of pre- and post-workout supplementa-tion had no significant effect on improving body composi-tion measures in trained CrossFit athletes. However, therewas a significant improvement in WOD performancewhich is a critical consideration for competitive CrossFitathletes. Although not significant, the SUP yielded a 2.04%increase in LBM, which may be of practical significancefor these athletes. This is the first study to investigate thepotential benefit of a practical pre and post-WOD supple-mentation on CrossFit performance measures. Additionalresearch is needed to better understand the potential fornutrition supplementation to optimize performance.

AcknowledgementsThis study was supported by Dymatize Nutrition Sport Performance Institute.

Authors’ details1University of Mary Hardin-Baylor, Human Performance Lab, 900 CollegeStreet, Belton, TX 76531, USA. 2Dymatize Nutrition Sport PerformanceInstitute, Dallas, TX 75234, USA. 3University of North Carolina Chapel Hill,Chapel Hill, NC 27599, USA.

Published: 6 December 2013

doi:10.1186/1550-2783-10-S1-P28Cite this article as: Urbina et al.: Performance and body compositioneffects of a pre-workout supplement and post-workout protein intakein trained crossfit individuals. Journal of the International Society of SportsNutrition 2013 10(Suppl 1):P28.

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Urbina et al. Journal of the International Society of Sports Nutrition 2013, 10(Suppl 1):P28http://www.jissn.com/content/10/S1/P28

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