peptide mapping infographic · title: peptide mapping infographic subject: infographic 72150...
TRANSCRIPT
Oxidation of Methionine
D Y M P K
O
N-terminal Glutamine to pyroGlu
Q V Q L V
O
O
R
H2N NH
R + H2O O
Conjugation sites
D A C P K
N-linked
O-linked
N Y S T K
S D Y C K
Glycosylation
A C T Y K
A C D Y K
Sequence Variance
D C D Q K
A Y C S K
S-S
Disulfide Bridge location
P G K
P G
C-terminal Lysine clipping
O
β
α
O
NH
NHOH
isoAspD or
P E N N Y K
Deamidation of Asparagine
Points of interest on a peptide map
What do we look for when mapping biopharmaceuticals?
RT
A
C
D
Y
M
D
K
A
C
D
Y
K
Y
M
D
K
D
P
C
Y
K
C
D
K
Peptide Separation
m/z
A
C
D
Y
M
D
K
A
C
D
Y
K
Y
M
D
K
D
P
C
Y
K
C
D
K
Peptide Detection
A C D Y M D K
A C D Y K
Y M D K D P C Y K
C D K
N-terminus –
– C-terminus
Data Interpretation
Protein Digestion A C D Y K D P C Y K
Mapping a biopharmaceutical route
What are the steps involved in peptide mapping?
©2016 Thermo Fisher Scientific. All rights reserved. ST72150-EN 1016S
Learn more at: thermofisher.com/PeptideMaps
• Complete and reproducible protein digestion
• High resolution reproducible peptide separations
• HRAM MS and MS/MS
• Powerful yet user-friendly software
Choosing the correct tools for your journey
What are the key tools to confidently map a protein?
• Peptide mapping has the selectivity to simultaneously monitor many attributes or points of interest.
• This is important for maintaining the safety and efficacy of protein therapeutics