peptide mapping infographic · title: peptide mapping infographic subject: infographic 72150...

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Oxidation of Methionine D Y M P K O N-terminal Glutamine to pyroGlu Q V Q L V O O R H 2 N N H R + H 2 O O Conjugation sites D A C P K N-linked O-linked N Y S T K S D Y C K Glycosylation A C T Y K A C D Y K Sequence Variance D C D Q K A Y C S K S-S Disulfide Bridge location P G K P G C-terminal Lysine clipping O β α O N H N H OH isoAsp D or P E N N Y K Deamidation of Asparagine Points of interest on a peptide map What do we look for when mapping biopharmaceuticals? RT A C D Y M D K A C D Y K Y M D K D P C Y K C D K Peptide Separation m/z A C D Y M D K A C D Y K Y M D K D P C Y K C D K Peptide Detection A C D Y M D K A C D Y K Y M D K D P C Y K C D K N-terminus – – C-terminus Data Interpretation Protein Digestion A C D Y K D P C Y K Mapping a biopharmaceutical route What are the steps involved in peptide mapping? ©2016 Thermo Fisher Scientific. All rights reserved. ST72150-EN 1016S Learn more at: thermofisher.com/PeptideMaps • Complete and reproducible protein digestion • High resolution reproducible peptide separations • HRAM MS and MS/MS • Powerful yet user-friendly software Choosing the correct tools for your journey What are the key tools to confidently map a protein? • Peptide mapping has the selectivity to simultaneously monitor many attributes or points of interest. • This is important for maintaining the safety and efficacy of protein therapeutics

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Page 1: Peptide Mapping Infographic · Title: Peptide Mapping Infographic Subject: Infographic 72150 Keywords: ST72150; ST 72150; HRAM; MS; MS/MS; Mass Spectrometry; High Resolution Accurate

Oxidation of Methionine

D Y M P K

O

N-terminal Glutamine to pyroGlu

Q V Q L V

O

O

R

H2N NH

R + H2O O

Conjugation sites

D A C P K

N-linked

O-linked

N Y S T K

S D Y C K

Glycosylation

A C T Y K

A C D Y K

Sequence Variance

D C D Q K

A Y C S K

S-S

Disulfide Bridge location

P G K

P G

C-terminal Lysine clipping

O

β

α

O

NH

NHOH

isoAspD or

P E N N Y K

Deamidation of Asparagine

Points of interest on a peptide map

What do we look for when mapping biopharmaceuticals?

RT

A

C

D

Y

M

D

K

A

C

D

Y

K

Y

M

D

K

D

P

C

Y

K

C

D

K

Peptide Separation

m/z

A

C

D

Y

M

D

K

A

C

D

Y

K

Y

M

D

K

D

P

C

Y

K

C

D

K

Peptide Detection

A C D Y M D K

A C D Y K

Y M D K D P C Y K

C D K

N-terminus –

– C-terminus

Data Interpretation

Protein Digestion A C D Y K D P C Y K

Mapping a biopharmaceutical route

What are the steps involved in peptide mapping?

©2016 Thermo Fisher Scientific. All rights reserved. ST72150-EN 1016S

Learn more at: thermofisher.com/PeptideMaps

• Complete and reproducible protein digestion

• High resolution reproducible peptide separations

• HRAM MS and MS/MS

• Powerful yet user-friendly software

Choosing the correct tools for your journey

What are the key tools to confidently map a protein?

• Peptide mapping has the selectivity to simultaneously monitor many attributes or points of interest.

• This is important for maintaining the safety and efficacy of protein therapeutics