PcrMolecular Biology and Biotechnology Bartolome Fayet CcamaMolecular Biology and Biotechnology Bartolome Fayet CcamaMolecular Biology and Biotechnology Bartolome Fayet CcamaMolecular Biology and Biotechnology Bartolome Fayet CcamaMolecular Biology and Biotechnology Bartolome Fayet CcamaMolecular Biology and Biotechnology Bartolome Fayet CcamaMolecular Biology and Biotechnology Bartolome Fayet Ccama

Download PcrMolecular Biology and Biotechnology Bartolome Fayet CcamaMolecular Biology and Biotechnology Bartolome Fayet CcamaMolecular Biology and Biotechnology Bartolome Fayet CcamaMolecular Biology and Biotechnology Bartolome Fayet CcamaMolecular Biology and Biotechnology Bartolome Fayet CcamaMolecular Biology and Biotechnology Bartolome Fayet CcamaMolecular Biology and Biotechnology Bartolome Fayet Ccama

Post on 20-Feb-2018

214 views

Category:

Documents

0 download

Embed Size (px)

TRANSCRIPT

  • 7/24/2019 PcrMolecular Biology and Biotechnology Bartolome Fayet CcamaMolecular Biology and Biotechnology Bartolome Fa

    1/2

    REACTIVOS

    PCR-buffer(1) 10x 2.5

    MgCl2 2.5

    dNTPs

    Taq polimerasa (5uid!u")

    #gua $l%ra Pura(#$P) 1&'2

    2& 0'

    1*+2R 0'

    TOTAL 2*

    ADN 1

    Para 1 REACCIN (,")

  • 7/24/2019 PcrMolecular Biology and Biotechnology Bartolome Fayet CcamaMolecular Biology and Biotechnology Bartolome Fa

    2/2

    PROGRAMA DE AMPLIFICACIN

    1 CICLO:

    Pre- desa%uraliai/ +* - 5 mi.

    35 CICLOS:

    esa%uraliai/ +* - 30 segudos

    4ibridai/ 55 - *5 seg

    Polimeriai/ &2 C 1 mi 30 seg

    6x%. ial &2 C 10 miCoser7ai/ * 8C