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REACTIVOS

PCR-buffer(1) 10x 2.5

MgCl2 2.5

dNTPs

Taq polimerasa (5uid!u")

#gua $l%ra Pura(#$P) 1&'2

2& 0'

1*+2R 0'

TOTAL 2*

ADN 1

Para 1 REACCIN (,")

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PROGRAMA DE AMPLIFICACIN

1 CICLO:

Pre- desa%uraliai/ +* - 5 mi.

35 CICLOS:

esa%uraliai/ +* - 30 segudos

4ibridai/ 55 - *5 seg

Polimeriai/ &2 C 1 mi 30 seg

6x%. ial &2 C 10 miCoser7ai/ * 8C