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  • Pathology Research 2013A. Fixatives 3

    A-1. for Light Microscopy .......................................................................................3A-2. for Electron Microscopy .................................................................................5

    B. Tissue Dehydrating Solution 6

    C. Decalcifying Agent 7

    D. Intermediates (Replacing agents) 9

    D-1. Lemosol and Lemosol A (Ace) ................................................................9

    E. Embedding Medium 11

    F. Immunopen 12

    G. Stains 13

    G-1. General Staining .............................................................................................14G-2. Polysaccharide Staining ..............................................................................16G-3. Connective Tissue Staining ........................................................................19G-4. Staining of Fibrins and Glial Tissues .......................................................23G-5. Intracellular Inorganic Substance Staining ...........................................23G-6. Cytological Staining ......................................................................................24G-7. Blood Cell Staining ........................................................................................25G-8. Hard Tissue Staining (TRAP/ALP Stain Kit) ........................................26G-9. Other stains ......................................................................................................27

    H. Mounting Reagent 28

    H-1. Softmount ..........................................................................................................28

    I. Antigen Retrieval Reagent 28

    I-1. ImmunoSaver ...................................................................................................28

    J. Gene-Related Kits 29

    J-1. Apoptosis in situ Detection Kit Wako ......................................................... 29J-2. DNA Isolator PS Kit ....................................................................................... 30J-3. DNA Isolator PS-Rapid Reagent ............................................................. 30J-4. ISOGEN PB Kit ................................................................................................31

    K. Paraffin-embedded Tissue Sections 32

    K-1. HistoMap Series ............................................................................................. 32

  • Alphabetical Indexpage Description

    27 A Acridine Orange27 Acridine Yellow17 Alcian Blue Solution, pH 2.522 Aniline Blue Orange G Solution21 Aniline Blue Solution27 Aniline Blue Water Soluble28 Apathy's Mounting Media, Soluble29 Apoptosis in situ Detection Kit Wako29 Apoptosis Ladder Detection Kit Wako27 Auramine22 Azocarmine G Solution23 B Berlin Blue Staining Set27 Bismarck Brown5 Bouin Solution28 C Canada Balsam27 Carmine5 Carnoy Solution15 Carrazzi's Hematoxylin Solution15 Carrazzi's Hematoxylin Solution (2)27 Chlorazol Black E16 Cold Schiffs Reagent11 Collodion (5%)11 Collodion (10%)19 Colloidal Iron Stock Solution27 Congo Red27 Crystal Violet29 D DAB Tablet (DAB . 4HCl 5mg/Tablet)29 DAB Tablet (DAB . 4HCl 10mg/Tablet)29 DAB TRIS Tablet, pH7.68 Decalcifying Solution A (Plank-Rychlo protocol)8 Decalcifying Solution B (EDTA protocol)27 4',6-Diamidino-2-phenylindole Dihydrochloride n-Hydrate30 DNA Isolator PS Kit30 DNA Isolator PS-Rapid Reagent11 Dodecenylsuccinic Anhydride [DDSA]15 E Eosin Alcohol Solution, acid extract27 Eosin Y15 0.1% Eosin Y Ethanol Solution15 0.5% Eosin Y Ethanol Solution15 1% Eosin Y Solution11 Epon 17411 Epon 81211 Epon 81527 Erythrosine30 Ethanol (99.5)11 Ethylene Glycol Diglycidyl Ether27 F Fast Blue RR Salt3 10% Formalin Neutral Buffer Solution3 15% Formalin Neutral Buffer Solution3 20% Formalin Neutral Buffer Solution8 5% Formic Acid (for Decalcification)27 Fuchsin Acid27 Fuchsine Basic11 G Gelatin11 Gelatin, Capsules No.011 Gelatin, Capsules No.0027 Gentian Violet R25 Giemsa Stain Solution6 10% Glutaraldehyde Solution6 20% Glutaraldehyde Solution6 70% Glutaraldehyde Solution27 H Hematoxylin Monohydrate32 HistoMap Mouse Normal Cerebellum, Paraffin Embedded Tissue32 HistoMap Mouse Normal Cerebrum, Paraffin Embedded Tissue Section32 HistoMap Mouse Normal Heart, Paraffin Embedded Tissue Section32 HistoMap Mouse Normal Kidney, Paraffin Embedded Tissue Section32 HistoMap Mouse Normal Liver, Paraffin Embedded Tissue Section32 HistoMap Mouse Normal Lung, Paraffin Embedded Tissue Section32 HistoMap Mouse Normal Pancreas, Paraffin Embedded Tissue Section32 HistoMap Mouse Normal Rectum, Paraffin Embedded Tissue Section32 HistoMap Mouse Normal Spleen, Paraffin Embedded Tissue Section32 HistoMap Mouse Normal Stomach, Paraffin Embedded Tissue Section32 HistoMap Mouse Normal Testis, Paraffin Embedded Tissue Section32 HistoMap Rat Normal Cerebellum, Paraffin Embedded Tissue32 HistoMap Rat Normal Cerebrum, Paraffin Embedded Tissue Section32 HistoMap Rat Normal Heart, Paraffin Embedded Tissue Section32 HistoMap Rat Normal Kidney, Paraffin Embedded Tissue Section32 HistoMap Rat Normal Liver, Paraffin Embedded Tissue Section32 HistoMap Rat Normal Lung, Paraffin Embedded Tissue Section32 HistoMap Rat Normal Pancreas, Paraffin Embedded Tissue Section32 HistoMap Rat Normal Rectum, Paraffin Embedded Tissue Section32 HistoMap Rat Normal Spleen, Paraffin Embedded Tissue Section32 HistoMap Rat Normal Stomach, Paraffin Embedded Tissue Section32 HistoMap Rat Normal Testis, Paraffin Embedded Tissue Section8 10% Hydrochloric Acid (for Decalcification)

    page Description12 I Immunopen28 ImmunoSaver27 Indigo Carmine31 ISOGEN-LS31 ISOGEN PB Kit7 K Kalkitox

    10 L Lemosol

    10 Lemosol A (Ace)10 Lemosol Coagulant27 Light Green SF Yellowish27 M Malachite Green Oxalate14 Mayers Hematoxylin Solution14 Mayers Hematoxylin Solution (2)25 May-Grnwald Stain Solution25 May-Grnwald's Eosin Methylene Blue Solution27 Metanil Yellow27 Methyl Green27 Methyl Violet B11 Methyl-5-norbornene-2,3-dicarboxylic Anhydride [MNA]27 Methylene Blue Tetrahydrate4 Mildform 10N4 Mildform 10NM4 Mildform 15N4 Mildform 15NM4 Mildform 20N4 Mildform 20NM8 Morse Solution

    27 N Neutral Red27 Nile Blue Hydrogensulfate27 Nile Red8 8% Nitric Acid (for Decalcification)

    11 Nonenylsuccinic Anhydride [NSA]27 O Oil Red O27 Orange G21 0.8% Orange G Solution27 Orcein6 Osmium (VIII) Oxide6 2% Osmium (VIII) Oxide Solution6 4% Osmium (VIII) Oxide Solution

    24 P Papanicolaou EA100 Stain Solution24 Papanicolaou Hematoxylin Stain Solution24 Papanicolaou OG100 Stain Solution24 Papanicolaou-Gill's Hematoxylin (Gill-5) Stain Solution6 Paraformaldehyde5 4% Paraformaldehyde Phosphate Buffer Solution

    11 Pathoprep 54611 Pathoprep 56811 Pathoprep 58027 Phloxine B23 Phosphotungstic Acid Hematoxylin Solution5 PLP Solution Set

    21 Ponceau Xylidine, Fuchsin Acid, Azophloxine Solution30 2-Propanol27 Pyronine Y27 S Safranine16 Schiffs Reagent27 Sirius Red8 5% Sodium Sulfate Solution (for Decalcification)

    28 Softmount27 Sudan 127 Sudan 227 Sudan 327 Sudan 427 Sudan Black B27 T Thioflavin T27 Thionin Acetate6 Tissue Dehydration Solution 996 Tissue Dehydration Solution 100

    18 0.05% Toluidine Blue Solution (pH2.5)18 0.05% Toluidine Blue Solution (pH4.1)18 0.05% Toluidine Blue Solution (pH7.0)26 TRAP/ALP Stain Kit8 5% Trichloroacetic Acid Solution (for Decalcification)6 2,4,6-Trimethylpyridine [-Collidine]

    11 2,4,6-Tris (dimethylaminomethyl) phenol [DMP-30]20 V Van Gieson Solution F20 Van Gieson Solution P27 Victoria Blue B27 Victoria Blue 4R22 Victoria Blue Solution19 W Weigert's Iron Hematoxylin Staining Set27 Wright Stain, Powder25 Wright Stain Solution5 Z Zamboni Solution

  • Pathology Research 2013 3

    AFixatives

    A. Fixatives

    F ixation means a process by which when observing microscopically the ecological or pathological state of a tissue specimen taken from a dead or live body, the tissue specimen is preserved in as "intact" a state as possible.The objectives are to:l Stabilize the main constituent protein of the tissue specimen as fast as possible to stop autolysisl Minimize the degeneration and deformation of the tissue specimen caused by the effects of chemicals and heat during

    the subsequent specimen preparation process.

    A-1. for Light Microscopy

    Neutral buffered formalin Formalin solution is used in large quantities as a routine fixative in the preparation of tissue specimens for light microscopic observation. Formalin is decomposed to produce formic acid, which may affect some tissues. This neutral buffered formalin is adjusted to pH 7.4 approximately by the addition of sodium phosphate to formalin to eliminate the effect of acid on tissues.Not only is neutral buffered formalin a superior fixative for routine lab work, it is also beneficial for a number of special purposes. For example, neutral buffered formalin can be used to a certain extent for histochemical detection of enzymes, hemoglobin and other molecules; for fixing mucopolysaccharides, glycogens, neurofibrils and other tissue components that cannot be treated with regular formalin; and for electron microscopy.

    Product Name Composition Formaldehyde Content pH Penetration Strength

    10% Formalin Neutral Buffer Solution

    Formalin ......................................................................100 mLSodium phosphate, monobasic, Dihydrate ........4.5 gDisodium phosphate ...................................................6.5 gPrepare the 1 L solution to add ion-exchanged water

    3.7~4.3 w/w% 7.4~7.5 l Penetration strength is in the order of 20% > 15% > 10%.

    l Specimens must be placed in the 10% fixative for several days or more.

    l The 20% fixative allows fixation in one or two days.

    l The 15% solution is about halfway between the 10% and 20% solutions.

    15% Formalin Neutral Buffer

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