paper chromatography

10
3RD BIOTECHNIQUE BELGIUM Paper chromatography: spinach leaves

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3RD BIOTECHNIQUE BELGIUM

Paper chromatography: spinach leaves

The aim is to separate and identify the different substances in the mixture

Material:

AlcoholSpinach leavesChromatography paperScissorsPencilBeakerGraduated cylinderMortar and pestle

Use mortar and pestle

Grind the leaves into a fine pulp

Pour in the mortar some alcohol

Cut out strip of chromatography paper

Place green mixture at the bottom of the strip

Paper strip in alcohol solution (but not the green mixture)

Wait for the band of solvent to migrate to the top of the filter paper and then remove the paper

Chromatography is the separation of a mixture by passing it through a medium through which different parts of the mixture will move at

different rates

Carotenoids

Xanthophylls

Chlorophyll BChlorophyll A

The pigmentsHave different degrees of solubility

You may also use

Other leavesColor sticksRed cabbageRed leavesSalad…

Would you try to do that?