3RD BIOTECHNIQUE BELGIUM
Paper chromatography: spinach leaves
The aim is to separate and identify the different substances in the mixture
Material:
AlcoholSpinach leavesChromatography paperScissorsPencilBeakerGraduated cylinderMortar and pestle
Grind the leaves into a fine pulp
Pour in the mortar some alcohol
Cut out strip of chromatography paper
Place green mixture at the bottom of the strip
Paper strip in alcohol solution (but not the green mixture)
Wait for the band of solvent to migrate to the top of the filter paper and then remove the paper
Chromatography is the separation of a mixture by passing it through a medium through which different parts of the mixture will move at
different rates
Carotenoids
Xanthophylls
Chlorophyll BChlorophyll A
The pigmentsHave different degrees of solubility
You may also use
Other leavesColor sticksRed cabbageRed leavesSalad…
Would you try to do that?