oxford nanopore technologies · 2019-02-04 · diversity, as each read encompasses a full...

Oxford Nanopore Technologies Gene expression Transcript isoform expression and usage is a key source of variation between healthy and diseased tissues. Short sequencing reads offer a partial overview of gene expression. The rapid development of long read sequencing technologies opens the unique opportunity to gain an accurate representation of transcript diversity, as each read encompasses a full transcript Clark et al., 2018 1 Nanopore sequencing also provides Direct RNA and cDNA sequencing Reduce bias, detect base modifications and analyse RNA viruses Speed Rapid workflows and real-time results Low input requirements As little as 1 ng (PCR-cDNA) or 250 ng (direct cDNA) • Challenging quantification • Higher multimapping • Complex transcriptome assembly • Accurate quantification • Lower multimapping • Full-length isoforms • Easier transcriptome assembly • Fusion transcript detection Long nanopore reads provide the complete picture. Isoform characterisation Long-read nanopore sequencing delivers full-length transcripts, allowing accurate isoform characterisation and quantification. Download the white paper at nanoporetech.com 1. Clark et al. 2018. BioRxiv. doi: https://doi.org/10.1101/260562 Oxford Nanopore Technologies, the Wheel icon, GridION, MinIT and PromethION are registered trademarks of Oxford Nanopore Technologies in various countries. © 2019 Oxford Nanopore Technologies. All rights reserved. GridION, MinION and PromethION are currently for research use only. of multi-exonic human genes undergo alternative splicing 312 exons in the gene TTN — the most exons in a single human gene Precursor mRNA Exon 1 Exon 4 Exon 5 Exon 6 Exon 7 Exon 8 Exon 9 Exon 2 Exon 3 SHORT READS LONG READS 6.3 transcript isoforms, on average, per gene in the human genome

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Oxford Nanopore TechnologiesGene expression

Transcript isoform expression and usage is a key source of variation between healthy and diseased tissues.

Short sequencing reads offer a partial overview of gene expression.

The rapid development of long read sequencing

technologies opens the unique opportunity to gain an accurate

representation of transcript diversity, as each read

encompasses a full transcript

Clark et al., 20181

Nanopore sequencing also provides

Direct RNA and cDNA sequencingReduce bias, detect base modifications and analyse RNA viruses

SpeedRapid workflows and real-time results

Low input requirementsAs little as 1 ng (PCR-cDNA) or 250 ng (direct cDNA)

• Challenging quantification• Higher multimapping• Complex transcriptome assembly

• Accurate quantification• Lower multimapping• Full-length isoforms• Easier transcriptome assembly• Fusion transcript detection

Long nanopore reads provide the complete picture.

Isoform characterisation Long-read nanopore sequencing delivers full-length transcripts, allowing accurate isoform characterisation and quantification.

Download the white paper at nanoporetech.com

1. Clark et al. 2018. BioRxiv. doi: https://doi.org/10.1101/260562

Oxford Nanopore Technologies, the Wheel icon, GridION, MinIT and PromethION are registered trademarks of Oxford Nanopore Technologies in various countries. © 2019 Oxford Nanopore Technologies. All rights reserved. GridION, MinION and PromethION are currently for research use only.

of multi-exonic human genes

undergo alternative splicing

312exons in the gene TTN — the most exons in a single

human gene

Precursor mRNA

Exon 1 Exon 4 Exon 5 Exon 6 Exon 7 Exon 8 Exon 9Exon 2 Exon 3

6.3transcript

isoforms, on average, per gene

in the human genome

Detecting inosine in nanopore sequencing data with machine

Nanopore sequencing - recap - Analysis of Next-Generation

NANOPORE SEQUENCING BASECALLING · NANOPORE SEQUENCING BASECALLING Wojciech Makałowski Institute of Bioinformatics, University of Muenster, Germany Department of Computational Biology,