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New HABs monitoring tools & approaches for developing and maintaining collaborative networks USEPA Region 10 HABs workshop Alan Wilson Fisheries - Auburn University 29-20 March 2016 Aquaculture pond Alabama, August 2008

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New HABs monitoring tools amp approaches for developing and

maintaining collaborative networksUSEPA Region 10 HABs workshop

Alan Wilson

Fisheries - Auburn University

29-20 March 2016

Aquaculture pondAlabama August 2008

Outlinebull New HABs monitoring tools

bull CellScope

bull Phycocyanin

bull qPCR

bull Environmental Sample Processor (ESP)

bull Flow cytobots

bull Toxin test strips and tube kits

bull Real-time databull Forecasting websites

Lake Fox16 January 2016

03 m Secchi depth

What is a bloom

Auburn Univ pond7 August 2009

What is a bloom

Fayette County Fishing Lake10 August 2009024 m Secchi depth118 μgL chlorophyll

What is a bloom

bull World Health Organization

bull httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Chorus and Bartram 1999

WHO ThresholdsCell density ge 20000 cellsmlChlorophyll ge 10 microgLMicrocystin drinking water ge1 microgL

What is a bloom

bull World Health Organization

bull US Environmental Protection Agency

bull httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

USEPA drinking water advisoriesmicrocystin

03 microgL children 0-6yrs16 microgL gt6yrs

cylindrospermopsin07 microgL children 0-6yrs30 microgL gt6yrs

What is a bloom

bull World Health Organization

bull US Environmental Protection Agency

bull Carlson Trophic State Index (TSI)bull widely used classification scheme

bull based on transparency (Secchi depth) chlorophyll a total phosphorus to relate to algal biomass typically during the summer

bull scale from 0 ndash 110 increase of 10 TSI units = 2x algal biomassbull TSI useful for comparing lakes within a region and for assessing

changes in trophic status over timebull httpaslonetlotocvol_22issue_20361pdf

What is a bloom

TSI values vs water quality

lt40 Oligotrophic clear water high hypolimnetic O2 year-round but possible anoxia in the deeper hypolimnion part of year

40-50 Mesotrophic moderately clear water possible hypolimnetic anoxia in summer andor under ice Fully supportive of all swimmable aesthetic uses possible cold-water fishery

50-60 Mildly eutrophic decreased secchi anoxic hypolimnion possible macrophyte ldquoproblemsrdquo warm-water fishery supportive of all swimmable aesthetic uses but ldquothreatenedrdquo

60-70 blue-green algal dominance with scums possible extensive macrophyte problems not supportive of all beneficial uses

gt70 Heavy blooms and scums in summer likely dense ldquoweedrdquo beds hypereutrophic possible fish kills fewer plant beds due to high algae not supportive of many beneficial uses

Carlson 1977 Limnology and Oceanography

TSI values

Oligotrophic Mesotrophic Eutrophic Hypereutrophic

Trophic state index

Secchi depth(m)

Chlorophyll(microgL)

Total Phosphorus

(microgL)

NOT A BLOOM

BLOOM

Carlson 1977 Limnology and Oceanography

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

What is a bloom

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Lakesidemonitoringapproaches

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Lake Fox16 January 2016

03 m Secchi depth

bull Important targetsbull Toxigenic cyanobacteria

bull Toxins (cell-bound andor dissolved)

bull Poor transparency

MicrocystisChroococcus Planktothrix

Cylindrospermopsis

Anabaena

Merismopedia Lyngbya Coelosphaerium

How do we quantify cyanobacterial abundance

Huge size range

Kalff 2002 Limnology

Algal group Length (microm)

PicoplanktonNanoplanktonMicroplanktonMesoplankton

02-2020-3030-200

200-20000

Burj Khalifa in Dubai (830m)

Picoplankton Mesoplankton

100000xdifference

Human (2m)

Stacked 240x to reach 200000m

Common techniques for enumerating phytoplankton

Hydrobios settling chambers

High performanceliquid chromatography

Flow CAM imaging particle analyzer

Quantitative PCR

Benchtop fluorometerTurner Designs Trilogy

Field sondeHydrolab minisonde

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

Common toxin analytical approaches

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Common toxin analytical approaches

Outlinebull New HABs monitoring tools

bull CellScope

bull Phycocyanin

bull qPCR

bull Environmental Sample Processor (ESP)

bull Flow cytobots

bull Toxin test strips and tube kits

bull Real-time databull Forecasting websites

Outlinebull New HABs monitoring tools

httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf

Seltenrich 2014

CellScope

bull httpcellscopeberkeleyedu

Phycocyanin approaches

Turner Designs Trilogy

Orange module630nm excitation660nm emission

Field sondeHydrolab minisonde

Phycocyanin benchtop method development

bull Field sampling

ndash September 2012 ndash 16 ponds (pond survey)

Auburn University pond facility

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Outlinebull New HABs monitoring tools

bull CellScope

bull Phycocyanin

bull qPCR

bull Environmental Sample Processor (ESP)

bull Flow cytobots

bull Toxin test strips and tube kits

bull Real-time databull Forecasting websites

Lake Fox16 January 2016

03 m Secchi depth

What is a bloom

Auburn Univ pond7 August 2009

What is a bloom

Fayette County Fishing Lake10 August 2009024 m Secchi depth118 μgL chlorophyll

What is a bloom

bull World Health Organization

bull httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Chorus and Bartram 1999

WHO ThresholdsCell density ge 20000 cellsmlChlorophyll ge 10 microgLMicrocystin drinking water ge1 microgL

What is a bloom

bull World Health Organization

bull US Environmental Protection Agency

bull httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

USEPA drinking water advisoriesmicrocystin

03 microgL children 0-6yrs16 microgL gt6yrs

cylindrospermopsin07 microgL children 0-6yrs30 microgL gt6yrs

What is a bloom

bull World Health Organization

bull US Environmental Protection Agency

bull Carlson Trophic State Index (TSI)bull widely used classification scheme

bull based on transparency (Secchi depth) chlorophyll a total phosphorus to relate to algal biomass typically during the summer

bull scale from 0 ndash 110 increase of 10 TSI units = 2x algal biomassbull TSI useful for comparing lakes within a region and for assessing

changes in trophic status over timebull httpaslonetlotocvol_22issue_20361pdf

What is a bloom

TSI values vs water quality

lt40 Oligotrophic clear water high hypolimnetic O2 year-round but possible anoxia in the deeper hypolimnion part of year

40-50 Mesotrophic moderately clear water possible hypolimnetic anoxia in summer andor under ice Fully supportive of all swimmable aesthetic uses possible cold-water fishery

50-60 Mildly eutrophic decreased secchi anoxic hypolimnion possible macrophyte ldquoproblemsrdquo warm-water fishery supportive of all swimmable aesthetic uses but ldquothreatenedrdquo

60-70 blue-green algal dominance with scums possible extensive macrophyte problems not supportive of all beneficial uses

gt70 Heavy blooms and scums in summer likely dense ldquoweedrdquo beds hypereutrophic possible fish kills fewer plant beds due to high algae not supportive of many beneficial uses

Carlson 1977 Limnology and Oceanography

TSI values

Oligotrophic Mesotrophic Eutrophic Hypereutrophic

Trophic state index

Secchi depth(m)

Chlorophyll(microgL)

Total Phosphorus

(microgL)

NOT A BLOOM

BLOOM

Carlson 1977 Limnology and Oceanography

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

What is a bloom

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Lakesidemonitoringapproaches

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Lake Fox16 January 2016

03 m Secchi depth

bull Important targetsbull Toxigenic cyanobacteria

bull Toxins (cell-bound andor dissolved)

bull Poor transparency

MicrocystisChroococcus Planktothrix

Cylindrospermopsis

Anabaena

Merismopedia Lyngbya Coelosphaerium

How do we quantify cyanobacterial abundance

Huge size range

Kalff 2002 Limnology

Algal group Length (microm)

PicoplanktonNanoplanktonMicroplanktonMesoplankton

02-2020-3030-200

200-20000

Burj Khalifa in Dubai (830m)

Picoplankton Mesoplankton

100000xdifference

Human (2m)

Stacked 240x to reach 200000m

Common techniques for enumerating phytoplankton

Hydrobios settling chambers

High performanceliquid chromatography

Flow CAM imaging particle analyzer

Quantitative PCR

Benchtop fluorometerTurner Designs Trilogy

Field sondeHydrolab minisonde

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

Common toxin analytical approaches

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Common toxin analytical approaches

Outlinebull New HABs monitoring tools

bull CellScope

bull Phycocyanin

bull qPCR

bull Environmental Sample Processor (ESP)

bull Flow cytobots

bull Toxin test strips and tube kits

bull Real-time databull Forecasting websites

Outlinebull New HABs monitoring tools

httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf

Seltenrich 2014

CellScope

bull httpcellscopeberkeleyedu

Phycocyanin approaches

Turner Designs Trilogy

Orange module630nm excitation660nm emission

Field sondeHydrolab minisonde

Phycocyanin benchtop method development

bull Field sampling

ndash September 2012 ndash 16 ponds (pond survey)

Auburn University pond facility

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Lake Fox16 January 2016

03 m Secchi depth

What is a bloom

Auburn Univ pond7 August 2009

What is a bloom

Fayette County Fishing Lake10 August 2009024 m Secchi depth118 μgL chlorophyll

What is a bloom

bull World Health Organization

bull httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Chorus and Bartram 1999

WHO ThresholdsCell density ge 20000 cellsmlChlorophyll ge 10 microgLMicrocystin drinking water ge1 microgL

What is a bloom

bull World Health Organization

bull US Environmental Protection Agency

bull httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

USEPA drinking water advisoriesmicrocystin

03 microgL children 0-6yrs16 microgL gt6yrs

cylindrospermopsin07 microgL children 0-6yrs30 microgL gt6yrs

What is a bloom

bull World Health Organization

bull US Environmental Protection Agency

bull Carlson Trophic State Index (TSI)bull widely used classification scheme

bull based on transparency (Secchi depth) chlorophyll a total phosphorus to relate to algal biomass typically during the summer

bull scale from 0 ndash 110 increase of 10 TSI units = 2x algal biomassbull TSI useful for comparing lakes within a region and for assessing

changes in trophic status over timebull httpaslonetlotocvol_22issue_20361pdf

What is a bloom

TSI values vs water quality

lt40 Oligotrophic clear water high hypolimnetic O2 year-round but possible anoxia in the deeper hypolimnion part of year

40-50 Mesotrophic moderately clear water possible hypolimnetic anoxia in summer andor under ice Fully supportive of all swimmable aesthetic uses possible cold-water fishery

50-60 Mildly eutrophic decreased secchi anoxic hypolimnion possible macrophyte ldquoproblemsrdquo warm-water fishery supportive of all swimmable aesthetic uses but ldquothreatenedrdquo

60-70 blue-green algal dominance with scums possible extensive macrophyte problems not supportive of all beneficial uses

gt70 Heavy blooms and scums in summer likely dense ldquoweedrdquo beds hypereutrophic possible fish kills fewer plant beds due to high algae not supportive of many beneficial uses

Carlson 1977 Limnology and Oceanography

TSI values

Oligotrophic Mesotrophic Eutrophic Hypereutrophic

Trophic state index

Secchi depth(m)

Chlorophyll(microgL)

Total Phosphorus

(microgL)

NOT A BLOOM

BLOOM

Carlson 1977 Limnology and Oceanography

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

What is a bloom

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Lakesidemonitoringapproaches

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Lake Fox16 January 2016

03 m Secchi depth

bull Important targetsbull Toxigenic cyanobacteria

bull Toxins (cell-bound andor dissolved)

bull Poor transparency

MicrocystisChroococcus Planktothrix

Cylindrospermopsis

Anabaena

Merismopedia Lyngbya Coelosphaerium

How do we quantify cyanobacterial abundance

Huge size range

Kalff 2002 Limnology

Algal group Length (microm)

PicoplanktonNanoplanktonMicroplanktonMesoplankton

02-2020-3030-200

200-20000

Burj Khalifa in Dubai (830m)

Picoplankton Mesoplankton

100000xdifference

Human (2m)

Stacked 240x to reach 200000m

Common techniques for enumerating phytoplankton

Hydrobios settling chambers

High performanceliquid chromatography

Flow CAM imaging particle analyzer

Quantitative PCR

Benchtop fluorometerTurner Designs Trilogy

Field sondeHydrolab minisonde

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

Common toxin analytical approaches

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Common toxin analytical approaches

Outlinebull New HABs monitoring tools

bull CellScope

bull Phycocyanin

bull qPCR

bull Environmental Sample Processor (ESP)

bull Flow cytobots

bull Toxin test strips and tube kits

bull Real-time databull Forecasting websites

Outlinebull New HABs monitoring tools

httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf

Seltenrich 2014

CellScope

bull httpcellscopeberkeleyedu

Phycocyanin approaches

Turner Designs Trilogy

Orange module630nm excitation660nm emission

Field sondeHydrolab minisonde

Phycocyanin benchtop method development

bull Field sampling

ndash September 2012 ndash 16 ponds (pond survey)

Auburn University pond facility

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Auburn Univ pond7 August 2009

What is a bloom

Fayette County Fishing Lake10 August 2009024 m Secchi depth118 μgL chlorophyll

What is a bloom

bull World Health Organization

bull httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Chorus and Bartram 1999

WHO ThresholdsCell density ge 20000 cellsmlChlorophyll ge 10 microgLMicrocystin drinking water ge1 microgL

What is a bloom

bull World Health Organization

bull US Environmental Protection Agency

bull httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

USEPA drinking water advisoriesmicrocystin

03 microgL children 0-6yrs16 microgL gt6yrs

cylindrospermopsin07 microgL children 0-6yrs30 microgL gt6yrs

What is a bloom

bull World Health Organization

bull US Environmental Protection Agency

bull Carlson Trophic State Index (TSI)bull widely used classification scheme

bull based on transparency (Secchi depth) chlorophyll a total phosphorus to relate to algal biomass typically during the summer

bull scale from 0 ndash 110 increase of 10 TSI units = 2x algal biomassbull TSI useful for comparing lakes within a region and for assessing

changes in trophic status over timebull httpaslonetlotocvol_22issue_20361pdf

What is a bloom

TSI values vs water quality

lt40 Oligotrophic clear water high hypolimnetic O2 year-round but possible anoxia in the deeper hypolimnion part of year

40-50 Mesotrophic moderately clear water possible hypolimnetic anoxia in summer andor under ice Fully supportive of all swimmable aesthetic uses possible cold-water fishery

50-60 Mildly eutrophic decreased secchi anoxic hypolimnion possible macrophyte ldquoproblemsrdquo warm-water fishery supportive of all swimmable aesthetic uses but ldquothreatenedrdquo

60-70 blue-green algal dominance with scums possible extensive macrophyte problems not supportive of all beneficial uses

gt70 Heavy blooms and scums in summer likely dense ldquoweedrdquo beds hypereutrophic possible fish kills fewer plant beds due to high algae not supportive of many beneficial uses

Carlson 1977 Limnology and Oceanography

TSI values

Oligotrophic Mesotrophic Eutrophic Hypereutrophic

Trophic state index

Secchi depth(m)

Chlorophyll(microgL)

Total Phosphorus

(microgL)

NOT A BLOOM

BLOOM

Carlson 1977 Limnology and Oceanography

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

What is a bloom

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Lakesidemonitoringapproaches

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Lake Fox16 January 2016

03 m Secchi depth

bull Important targetsbull Toxigenic cyanobacteria

bull Toxins (cell-bound andor dissolved)

bull Poor transparency

MicrocystisChroococcus Planktothrix

Cylindrospermopsis

Anabaena

Merismopedia Lyngbya Coelosphaerium

How do we quantify cyanobacterial abundance

Huge size range

Kalff 2002 Limnology

Algal group Length (microm)

PicoplanktonNanoplanktonMicroplanktonMesoplankton

02-2020-3030-200

200-20000

Burj Khalifa in Dubai (830m)

Picoplankton Mesoplankton

100000xdifference

Human (2m)

Stacked 240x to reach 200000m

Common techniques for enumerating phytoplankton

Hydrobios settling chambers

High performanceliquid chromatography

Flow CAM imaging particle analyzer

Quantitative PCR

Benchtop fluorometerTurner Designs Trilogy

Field sondeHydrolab minisonde

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

Common toxin analytical approaches

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Common toxin analytical approaches

Outlinebull New HABs monitoring tools

bull CellScope

bull Phycocyanin

bull qPCR

bull Environmental Sample Processor (ESP)

bull Flow cytobots

bull Toxin test strips and tube kits

bull Real-time databull Forecasting websites

Outlinebull New HABs monitoring tools

httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf

Seltenrich 2014

CellScope

bull httpcellscopeberkeleyedu

Phycocyanin approaches

Turner Designs Trilogy

Orange module630nm excitation660nm emission

Field sondeHydrolab minisonde

Phycocyanin benchtop method development

bull Field sampling

ndash September 2012 ndash 16 ponds (pond survey)

Auburn University pond facility

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Fayette County Fishing Lake10 August 2009024 m Secchi depth118 μgL chlorophyll

What is a bloom

bull World Health Organization

bull httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Chorus and Bartram 1999

WHO ThresholdsCell density ge 20000 cellsmlChlorophyll ge 10 microgLMicrocystin drinking water ge1 microgL

What is a bloom

bull World Health Organization

bull US Environmental Protection Agency

bull httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

USEPA drinking water advisoriesmicrocystin

03 microgL children 0-6yrs16 microgL gt6yrs

cylindrospermopsin07 microgL children 0-6yrs30 microgL gt6yrs

What is a bloom

bull World Health Organization

bull US Environmental Protection Agency

bull Carlson Trophic State Index (TSI)bull widely used classification scheme

bull based on transparency (Secchi depth) chlorophyll a total phosphorus to relate to algal biomass typically during the summer

bull scale from 0 ndash 110 increase of 10 TSI units = 2x algal biomassbull TSI useful for comparing lakes within a region and for assessing

changes in trophic status over timebull httpaslonetlotocvol_22issue_20361pdf

What is a bloom

TSI values vs water quality

lt40 Oligotrophic clear water high hypolimnetic O2 year-round but possible anoxia in the deeper hypolimnion part of year

40-50 Mesotrophic moderately clear water possible hypolimnetic anoxia in summer andor under ice Fully supportive of all swimmable aesthetic uses possible cold-water fishery

50-60 Mildly eutrophic decreased secchi anoxic hypolimnion possible macrophyte ldquoproblemsrdquo warm-water fishery supportive of all swimmable aesthetic uses but ldquothreatenedrdquo

60-70 blue-green algal dominance with scums possible extensive macrophyte problems not supportive of all beneficial uses

gt70 Heavy blooms and scums in summer likely dense ldquoweedrdquo beds hypereutrophic possible fish kills fewer plant beds due to high algae not supportive of many beneficial uses

Carlson 1977 Limnology and Oceanography

TSI values

Oligotrophic Mesotrophic Eutrophic Hypereutrophic

Trophic state index

Secchi depth(m)

Chlorophyll(microgL)

Total Phosphorus

(microgL)

NOT A BLOOM

BLOOM

Carlson 1977 Limnology and Oceanography

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

What is a bloom

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Lakesidemonitoringapproaches

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Lake Fox16 January 2016

03 m Secchi depth

bull Important targetsbull Toxigenic cyanobacteria

bull Toxins (cell-bound andor dissolved)

bull Poor transparency

MicrocystisChroococcus Planktothrix

Cylindrospermopsis

Anabaena

Merismopedia Lyngbya Coelosphaerium

How do we quantify cyanobacterial abundance

Huge size range

Kalff 2002 Limnology

Algal group Length (microm)

PicoplanktonNanoplanktonMicroplanktonMesoplankton

02-2020-3030-200

200-20000

Burj Khalifa in Dubai (830m)

Picoplankton Mesoplankton

100000xdifference

Human (2m)

Stacked 240x to reach 200000m

Common techniques for enumerating phytoplankton

Hydrobios settling chambers

High performanceliquid chromatography

Flow CAM imaging particle analyzer

Quantitative PCR

Benchtop fluorometerTurner Designs Trilogy

Field sondeHydrolab minisonde

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

Common toxin analytical approaches

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Common toxin analytical approaches

Outlinebull New HABs monitoring tools

bull CellScope

bull Phycocyanin

bull qPCR

bull Environmental Sample Processor (ESP)

bull Flow cytobots

bull Toxin test strips and tube kits

bull Real-time databull Forecasting websites

Outlinebull New HABs monitoring tools

httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf

Seltenrich 2014

CellScope

bull httpcellscopeberkeleyedu

Phycocyanin approaches

Turner Designs Trilogy

Orange module630nm excitation660nm emission

Field sondeHydrolab minisonde

Phycocyanin benchtop method development

bull Field sampling

ndash September 2012 ndash 16 ponds (pond survey)

Auburn University pond facility

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

bull World Health Organization

bull httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Chorus and Bartram 1999

WHO ThresholdsCell density ge 20000 cellsmlChlorophyll ge 10 microgLMicrocystin drinking water ge1 microgL

What is a bloom

bull World Health Organization

bull US Environmental Protection Agency

bull httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

USEPA drinking water advisoriesmicrocystin

03 microgL children 0-6yrs16 microgL gt6yrs

cylindrospermopsin07 microgL children 0-6yrs30 microgL gt6yrs

What is a bloom

bull World Health Organization

bull US Environmental Protection Agency

bull Carlson Trophic State Index (TSI)bull widely used classification scheme

bull based on transparency (Secchi depth) chlorophyll a total phosphorus to relate to algal biomass typically during the summer

bull scale from 0 ndash 110 increase of 10 TSI units = 2x algal biomassbull TSI useful for comparing lakes within a region and for assessing

changes in trophic status over timebull httpaslonetlotocvol_22issue_20361pdf

What is a bloom

TSI values vs water quality

lt40 Oligotrophic clear water high hypolimnetic O2 year-round but possible anoxia in the deeper hypolimnion part of year

40-50 Mesotrophic moderately clear water possible hypolimnetic anoxia in summer andor under ice Fully supportive of all swimmable aesthetic uses possible cold-water fishery

50-60 Mildly eutrophic decreased secchi anoxic hypolimnion possible macrophyte ldquoproblemsrdquo warm-water fishery supportive of all swimmable aesthetic uses but ldquothreatenedrdquo

60-70 blue-green algal dominance with scums possible extensive macrophyte problems not supportive of all beneficial uses

gt70 Heavy blooms and scums in summer likely dense ldquoweedrdquo beds hypereutrophic possible fish kills fewer plant beds due to high algae not supportive of many beneficial uses

Carlson 1977 Limnology and Oceanography

TSI values

Oligotrophic Mesotrophic Eutrophic Hypereutrophic

Trophic state index

Secchi depth(m)

Chlorophyll(microgL)

Total Phosphorus

(microgL)

NOT A BLOOM

BLOOM

Carlson 1977 Limnology and Oceanography

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

What is a bloom

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Lakesidemonitoringapproaches

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Lake Fox16 January 2016

03 m Secchi depth

bull Important targetsbull Toxigenic cyanobacteria

bull Toxins (cell-bound andor dissolved)

bull Poor transparency

MicrocystisChroococcus Planktothrix

Cylindrospermopsis

Anabaena

Merismopedia Lyngbya Coelosphaerium

How do we quantify cyanobacterial abundance

Huge size range

Kalff 2002 Limnology

Algal group Length (microm)

PicoplanktonNanoplanktonMicroplanktonMesoplankton

02-2020-3030-200

200-20000

Burj Khalifa in Dubai (830m)

Picoplankton Mesoplankton

100000xdifference

Human (2m)

Stacked 240x to reach 200000m

Common techniques for enumerating phytoplankton

Hydrobios settling chambers

High performanceliquid chromatography

Flow CAM imaging particle analyzer

Quantitative PCR

Benchtop fluorometerTurner Designs Trilogy

Field sondeHydrolab minisonde

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

Common toxin analytical approaches

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Common toxin analytical approaches

Outlinebull New HABs monitoring tools

bull CellScope

bull Phycocyanin

bull qPCR

bull Environmental Sample Processor (ESP)

bull Flow cytobots

bull Toxin test strips and tube kits

bull Real-time databull Forecasting websites

Outlinebull New HABs monitoring tools

httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf

Seltenrich 2014

CellScope

bull httpcellscopeberkeleyedu

Phycocyanin approaches

Turner Designs Trilogy

Orange module630nm excitation660nm emission

Field sondeHydrolab minisonde

Phycocyanin benchtop method development

bull Field sampling

ndash September 2012 ndash 16 ponds (pond survey)

Auburn University pond facility

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

bull World Health Organization

bull US Environmental Protection Agency

bull httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

USEPA drinking water advisoriesmicrocystin

03 microgL children 0-6yrs16 microgL gt6yrs

cylindrospermopsin07 microgL children 0-6yrs30 microgL gt6yrs

What is a bloom

bull World Health Organization

bull US Environmental Protection Agency

bull Carlson Trophic State Index (TSI)bull widely used classification scheme

bull based on transparency (Secchi depth) chlorophyll a total phosphorus to relate to algal biomass typically during the summer

bull scale from 0 ndash 110 increase of 10 TSI units = 2x algal biomassbull TSI useful for comparing lakes within a region and for assessing

changes in trophic status over timebull httpaslonetlotocvol_22issue_20361pdf

What is a bloom

TSI values vs water quality

lt40 Oligotrophic clear water high hypolimnetic O2 year-round but possible anoxia in the deeper hypolimnion part of year

40-50 Mesotrophic moderately clear water possible hypolimnetic anoxia in summer andor under ice Fully supportive of all swimmable aesthetic uses possible cold-water fishery

50-60 Mildly eutrophic decreased secchi anoxic hypolimnion possible macrophyte ldquoproblemsrdquo warm-water fishery supportive of all swimmable aesthetic uses but ldquothreatenedrdquo

60-70 blue-green algal dominance with scums possible extensive macrophyte problems not supportive of all beneficial uses

gt70 Heavy blooms and scums in summer likely dense ldquoweedrdquo beds hypereutrophic possible fish kills fewer plant beds due to high algae not supportive of many beneficial uses

Carlson 1977 Limnology and Oceanography

TSI values

Oligotrophic Mesotrophic Eutrophic Hypereutrophic

Trophic state index

Secchi depth(m)

Chlorophyll(microgL)

Total Phosphorus

(microgL)

NOT A BLOOM

BLOOM

Carlson 1977 Limnology and Oceanography

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

What is a bloom

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Lakesidemonitoringapproaches

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Lake Fox16 January 2016

03 m Secchi depth

bull Important targetsbull Toxigenic cyanobacteria

bull Toxins (cell-bound andor dissolved)

bull Poor transparency

MicrocystisChroococcus Planktothrix

Cylindrospermopsis

Anabaena

Merismopedia Lyngbya Coelosphaerium

How do we quantify cyanobacterial abundance

Huge size range

Kalff 2002 Limnology

Algal group Length (microm)

PicoplanktonNanoplanktonMicroplanktonMesoplankton

02-2020-3030-200

200-20000

Burj Khalifa in Dubai (830m)

Picoplankton Mesoplankton

100000xdifference

Human (2m)

Stacked 240x to reach 200000m

Common techniques for enumerating phytoplankton

Hydrobios settling chambers

High performanceliquid chromatography

Flow CAM imaging particle analyzer

Quantitative PCR

Benchtop fluorometerTurner Designs Trilogy

Field sondeHydrolab minisonde

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

Common toxin analytical approaches

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Common toxin analytical approaches

Outlinebull New HABs monitoring tools

bull CellScope

bull Phycocyanin

bull qPCR

bull Environmental Sample Processor (ESP)

bull Flow cytobots

bull Toxin test strips and tube kits

bull Real-time databull Forecasting websites

Outlinebull New HABs monitoring tools

httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf

Seltenrich 2014

CellScope

bull httpcellscopeberkeleyedu

Phycocyanin approaches

Turner Designs Trilogy

Orange module630nm excitation660nm emission

Field sondeHydrolab minisonde

Phycocyanin benchtop method development

bull Field sampling

ndash September 2012 ndash 16 ponds (pond survey)

Auburn University pond facility

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

bull World Health Organization

bull US Environmental Protection Agency

bull Carlson Trophic State Index (TSI)bull widely used classification scheme

bull based on transparency (Secchi depth) chlorophyll a total phosphorus to relate to algal biomass typically during the summer

bull scale from 0 ndash 110 increase of 10 TSI units = 2x algal biomassbull TSI useful for comparing lakes within a region and for assessing

changes in trophic status over timebull httpaslonetlotocvol_22issue_20361pdf

What is a bloom

TSI values vs water quality

lt40 Oligotrophic clear water high hypolimnetic O2 year-round but possible anoxia in the deeper hypolimnion part of year

40-50 Mesotrophic moderately clear water possible hypolimnetic anoxia in summer andor under ice Fully supportive of all swimmable aesthetic uses possible cold-water fishery

50-60 Mildly eutrophic decreased secchi anoxic hypolimnion possible macrophyte ldquoproblemsrdquo warm-water fishery supportive of all swimmable aesthetic uses but ldquothreatenedrdquo

60-70 blue-green algal dominance with scums possible extensive macrophyte problems not supportive of all beneficial uses

gt70 Heavy blooms and scums in summer likely dense ldquoweedrdquo beds hypereutrophic possible fish kills fewer plant beds due to high algae not supportive of many beneficial uses

Carlson 1977 Limnology and Oceanography

TSI values

Oligotrophic Mesotrophic Eutrophic Hypereutrophic

Trophic state index

Secchi depth(m)

Chlorophyll(microgL)

Total Phosphorus

(microgL)

NOT A BLOOM

BLOOM

Carlson 1977 Limnology and Oceanography

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

What is a bloom

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Lakesidemonitoringapproaches

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Lake Fox16 January 2016

03 m Secchi depth

bull Important targetsbull Toxigenic cyanobacteria

bull Toxins (cell-bound andor dissolved)

bull Poor transparency

MicrocystisChroococcus Planktothrix

Cylindrospermopsis

Anabaena

Merismopedia Lyngbya Coelosphaerium

How do we quantify cyanobacterial abundance

Huge size range

Kalff 2002 Limnology

Algal group Length (microm)

PicoplanktonNanoplanktonMicroplanktonMesoplankton

02-2020-3030-200

200-20000

Burj Khalifa in Dubai (830m)

Picoplankton Mesoplankton

100000xdifference

Human (2m)

Stacked 240x to reach 200000m

Common techniques for enumerating phytoplankton

Hydrobios settling chambers

High performanceliquid chromatography

Flow CAM imaging particle analyzer

Quantitative PCR

Benchtop fluorometerTurner Designs Trilogy

Field sondeHydrolab minisonde

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

Common toxin analytical approaches

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Common toxin analytical approaches

Outlinebull New HABs monitoring tools

bull CellScope

bull Phycocyanin

bull qPCR

bull Environmental Sample Processor (ESP)

bull Flow cytobots

bull Toxin test strips and tube kits

bull Real-time databull Forecasting websites

Outlinebull New HABs monitoring tools

httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf

Seltenrich 2014

CellScope

bull httpcellscopeberkeleyedu

Phycocyanin approaches

Turner Designs Trilogy

Orange module630nm excitation660nm emission

Field sondeHydrolab minisonde

Phycocyanin benchtop method development

bull Field sampling

ndash September 2012 ndash 16 ponds (pond survey)

Auburn University pond facility

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

TSI values vs water quality

lt40 Oligotrophic clear water high hypolimnetic O2 year-round but possible anoxia in the deeper hypolimnion part of year

40-50 Mesotrophic moderately clear water possible hypolimnetic anoxia in summer andor under ice Fully supportive of all swimmable aesthetic uses possible cold-water fishery

50-60 Mildly eutrophic decreased secchi anoxic hypolimnion possible macrophyte ldquoproblemsrdquo warm-water fishery supportive of all swimmable aesthetic uses but ldquothreatenedrdquo

60-70 blue-green algal dominance with scums possible extensive macrophyte problems not supportive of all beneficial uses

gt70 Heavy blooms and scums in summer likely dense ldquoweedrdquo beds hypereutrophic possible fish kills fewer plant beds due to high algae not supportive of many beneficial uses

Carlson 1977 Limnology and Oceanography

TSI values

Oligotrophic Mesotrophic Eutrophic Hypereutrophic

Trophic state index

Secchi depth(m)

Chlorophyll(microgL)

Total Phosphorus

(microgL)

NOT A BLOOM

BLOOM

Carlson 1977 Limnology and Oceanography

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

What is a bloom

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Lakesidemonitoringapproaches

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Lake Fox16 January 2016

03 m Secchi depth

bull Important targetsbull Toxigenic cyanobacteria

bull Toxins (cell-bound andor dissolved)

bull Poor transparency

MicrocystisChroococcus Planktothrix

Cylindrospermopsis

Anabaena

Merismopedia Lyngbya Coelosphaerium

How do we quantify cyanobacterial abundance

Huge size range

Kalff 2002 Limnology

Algal group Length (microm)

PicoplanktonNanoplanktonMicroplanktonMesoplankton

02-2020-3030-200

200-20000

Burj Khalifa in Dubai (830m)

Picoplankton Mesoplankton

100000xdifference

Human (2m)

Stacked 240x to reach 200000m

Common techniques for enumerating phytoplankton

Hydrobios settling chambers

High performanceliquid chromatography

Flow CAM imaging particle analyzer

Quantitative PCR

Benchtop fluorometerTurner Designs Trilogy

Field sondeHydrolab minisonde

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

Common toxin analytical approaches

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Common toxin analytical approaches

Outlinebull New HABs monitoring tools

bull CellScope

bull Phycocyanin

bull qPCR

bull Environmental Sample Processor (ESP)

bull Flow cytobots

bull Toxin test strips and tube kits

bull Real-time databull Forecasting websites

Outlinebull New HABs monitoring tools

httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf

Seltenrich 2014

CellScope

bull httpcellscopeberkeleyedu

Phycocyanin approaches

Turner Designs Trilogy

Orange module630nm excitation660nm emission

Field sondeHydrolab minisonde

Phycocyanin benchtop method development

bull Field sampling

ndash September 2012 ndash 16 ponds (pond survey)

Auburn University pond facility

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

TSI values

Oligotrophic Mesotrophic Eutrophic Hypereutrophic

Trophic state index

Secchi depth(m)

Chlorophyll(microgL)

Total Phosphorus

(microgL)

NOT A BLOOM

BLOOM

Carlson 1977 Limnology and Oceanography

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

What is a bloom

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Lakesidemonitoringapproaches

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Lake Fox16 January 2016

03 m Secchi depth

bull Important targetsbull Toxigenic cyanobacteria

bull Toxins (cell-bound andor dissolved)

bull Poor transparency

MicrocystisChroococcus Planktothrix

Cylindrospermopsis

Anabaena

Merismopedia Lyngbya Coelosphaerium

How do we quantify cyanobacterial abundance

Huge size range

Kalff 2002 Limnology

Algal group Length (microm)

PicoplanktonNanoplanktonMicroplanktonMesoplankton

02-2020-3030-200

200-20000

Burj Khalifa in Dubai (830m)

Picoplankton Mesoplankton

100000xdifference

Human (2m)

Stacked 240x to reach 200000m

Common techniques for enumerating phytoplankton

Hydrobios settling chambers

High performanceliquid chromatography

Flow CAM imaging particle analyzer

Quantitative PCR

Benchtop fluorometerTurner Designs Trilogy

Field sondeHydrolab minisonde

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

Common toxin analytical approaches

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Common toxin analytical approaches

Outlinebull New HABs monitoring tools

bull CellScope

bull Phycocyanin

bull qPCR

bull Environmental Sample Processor (ESP)

bull Flow cytobots

bull Toxin test strips and tube kits

bull Real-time databull Forecasting websites

Outlinebull New HABs monitoring tools

httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf

Seltenrich 2014

CellScope

bull httpcellscopeberkeleyedu

Phycocyanin approaches

Turner Designs Trilogy

Orange module630nm excitation660nm emission

Field sondeHydrolab minisonde

Phycocyanin benchtop method development

bull Field sampling

ndash September 2012 ndash 16 ponds (pond survey)

Auburn University pond facility

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

What is a bloom

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Lakesidemonitoringapproaches

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Lake Fox16 January 2016

03 m Secchi depth

bull Important targetsbull Toxigenic cyanobacteria

bull Toxins (cell-bound andor dissolved)

bull Poor transparency

MicrocystisChroococcus Planktothrix

Cylindrospermopsis

Anabaena

Merismopedia Lyngbya Coelosphaerium

How do we quantify cyanobacterial abundance

Huge size range

Kalff 2002 Limnology

Algal group Length (microm)

PicoplanktonNanoplanktonMicroplanktonMesoplankton

02-2020-3030-200

200-20000

Burj Khalifa in Dubai (830m)

Picoplankton Mesoplankton

100000xdifference

Human (2m)

Stacked 240x to reach 200000m

Common techniques for enumerating phytoplankton

Hydrobios settling chambers

High performanceliquid chromatography

Flow CAM imaging particle analyzer

Quantitative PCR

Benchtop fluorometerTurner Designs Trilogy

Field sondeHydrolab minisonde

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

Common toxin analytical approaches

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Common toxin analytical approaches

Outlinebull New HABs monitoring tools

bull CellScope

bull Phycocyanin

bull qPCR

bull Environmental Sample Processor (ESP)

bull Flow cytobots

bull Toxin test strips and tube kits

bull Real-time databull Forecasting websites

Outlinebull New HABs monitoring tools

httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf

Seltenrich 2014

CellScope

bull httpcellscopeberkeleyedu

Phycocyanin approaches

Turner Designs Trilogy

Orange module630nm excitation660nm emission

Field sondeHydrolab minisonde

Phycocyanin benchtop method development

bull Field sampling

ndash September 2012 ndash 16 ponds (pond survey)

Auburn University pond facility

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

What is a bloom

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Lakesidemonitoringapproaches

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Lake Fox16 January 2016

03 m Secchi depth

bull Important targetsbull Toxigenic cyanobacteria

bull Toxins (cell-bound andor dissolved)

bull Poor transparency

MicrocystisChroococcus Planktothrix

Cylindrospermopsis

Anabaena

Merismopedia Lyngbya Coelosphaerium

How do we quantify cyanobacterial abundance

Huge size range

Kalff 2002 Limnology

Algal group Length (microm)

PicoplanktonNanoplanktonMicroplanktonMesoplankton

02-2020-3030-200

200-20000

Burj Khalifa in Dubai (830m)

Picoplankton Mesoplankton

100000xdifference

Human (2m)

Stacked 240x to reach 200000m

Common techniques for enumerating phytoplankton

Hydrobios settling chambers

High performanceliquid chromatography

Flow CAM imaging particle analyzer

Quantitative PCR

Benchtop fluorometerTurner Designs Trilogy

Field sondeHydrolab minisonde

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

Common toxin analytical approaches

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Common toxin analytical approaches

Outlinebull New HABs monitoring tools

bull CellScope

bull Phycocyanin

bull qPCR

bull Environmental Sample Processor (ESP)

bull Flow cytobots

bull Toxin test strips and tube kits

bull Real-time databull Forecasting websites

Outlinebull New HABs monitoring tools

httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf

Seltenrich 2014

CellScope

bull httpcellscopeberkeleyedu

Phycocyanin approaches

Turner Designs Trilogy

Orange module630nm excitation660nm emission

Field sondeHydrolab minisonde

Phycocyanin benchtop method development

bull Field sampling

ndash September 2012 ndash 16 ponds (pond survey)

Auburn University pond facility

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

bull Go samplebull Integrated andor surface

samples

bull Whole water samples

bull Secchi depth

What is a bloom

Lake Fox16 January 2016

03 m Secchi depth

bull Important targetsbull Toxigenic cyanobacteria

bull Toxins (cell-bound andor dissolved)

bull Poor transparency

MicrocystisChroococcus Planktothrix

Cylindrospermopsis

Anabaena

Merismopedia Lyngbya Coelosphaerium

How do we quantify cyanobacterial abundance

Huge size range

Kalff 2002 Limnology

Algal group Length (microm)

PicoplanktonNanoplanktonMicroplanktonMesoplankton

02-2020-3030-200

200-20000

Burj Khalifa in Dubai (830m)

Picoplankton Mesoplankton

100000xdifference

Human (2m)

Stacked 240x to reach 200000m

Common techniques for enumerating phytoplankton

Hydrobios settling chambers

High performanceliquid chromatography

Flow CAM imaging particle analyzer

Quantitative PCR

Benchtop fluorometerTurner Designs Trilogy

Field sondeHydrolab minisonde

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

Common toxin analytical approaches

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Common toxin analytical approaches

Outlinebull New HABs monitoring tools

bull CellScope

bull Phycocyanin

bull qPCR

bull Environmental Sample Processor (ESP)

bull Flow cytobots

bull Toxin test strips and tube kits

bull Real-time databull Forecasting websites

Outlinebull New HABs monitoring tools

httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf

Seltenrich 2014

CellScope

bull httpcellscopeberkeleyedu

Phycocyanin approaches

Turner Designs Trilogy

Orange module630nm excitation660nm emission

Field sondeHydrolab minisonde

Phycocyanin benchtop method development

bull Field sampling

ndash September 2012 ndash 16 ponds (pond survey)

Auburn University pond facility

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

MicrocystisChroococcus Planktothrix

Cylindrospermopsis

Anabaena

Merismopedia Lyngbya Coelosphaerium

How do we quantify cyanobacterial abundance

Huge size range

Kalff 2002 Limnology

Algal group Length (microm)

PicoplanktonNanoplanktonMicroplanktonMesoplankton

02-2020-3030-200

200-20000

Burj Khalifa in Dubai (830m)

Picoplankton Mesoplankton

100000xdifference

Human (2m)

Stacked 240x to reach 200000m

Common techniques for enumerating phytoplankton

Hydrobios settling chambers

High performanceliquid chromatography

Flow CAM imaging particle analyzer

Quantitative PCR

Benchtop fluorometerTurner Designs Trilogy

Field sondeHydrolab minisonde

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

Common toxin analytical approaches

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Common toxin analytical approaches

Outlinebull New HABs monitoring tools

bull CellScope

bull Phycocyanin

bull qPCR

bull Environmental Sample Processor (ESP)

bull Flow cytobots

bull Toxin test strips and tube kits

bull Real-time databull Forecasting websites

Outlinebull New HABs monitoring tools

httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf

Seltenrich 2014

CellScope

bull httpcellscopeberkeleyedu

Phycocyanin approaches

Turner Designs Trilogy

Orange module630nm excitation660nm emission

Field sondeHydrolab minisonde

Phycocyanin benchtop method development

bull Field sampling

ndash September 2012 ndash 16 ponds (pond survey)

Auburn University pond facility

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Huge size range

Kalff 2002 Limnology

Algal group Length (microm)

PicoplanktonNanoplanktonMicroplanktonMesoplankton

02-2020-3030-200

200-20000

Burj Khalifa in Dubai (830m)

Picoplankton Mesoplankton

100000xdifference

Human (2m)

Stacked 240x to reach 200000m

Common techniques for enumerating phytoplankton

Hydrobios settling chambers

High performanceliquid chromatography

Flow CAM imaging particle analyzer

Quantitative PCR

Benchtop fluorometerTurner Designs Trilogy

Field sondeHydrolab minisonde

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

Common toxin analytical approaches

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Common toxin analytical approaches

Outlinebull New HABs monitoring tools

bull CellScope

bull Phycocyanin

bull qPCR

bull Environmental Sample Processor (ESP)

bull Flow cytobots

bull Toxin test strips and tube kits

bull Real-time databull Forecasting websites

Outlinebull New HABs monitoring tools

httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf

Seltenrich 2014

CellScope

bull httpcellscopeberkeleyedu

Phycocyanin approaches

Turner Designs Trilogy

Orange module630nm excitation660nm emission

Field sondeHydrolab minisonde

Phycocyanin benchtop method development

bull Field sampling

ndash September 2012 ndash 16 ponds (pond survey)

Auburn University pond facility

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Common techniques for enumerating phytoplankton

Hydrobios settling chambers

High performanceliquid chromatography

Flow CAM imaging particle analyzer

Quantitative PCR

Benchtop fluorometerTurner Designs Trilogy

Field sondeHydrolab minisonde

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

Common toxin analytical approaches

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Common toxin analytical approaches

Outlinebull New HABs monitoring tools

bull CellScope

bull Phycocyanin

bull qPCR

bull Environmental Sample Processor (ESP)

bull Flow cytobots

bull Toxin test strips and tube kits

bull Real-time databull Forecasting websites

Outlinebull New HABs monitoring tools

httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf

Seltenrich 2014

CellScope

bull httpcellscopeberkeleyedu

Phycocyanin approaches

Turner Designs Trilogy

Orange module630nm excitation660nm emission

Field sondeHydrolab minisonde

Phycocyanin benchtop method development

bull Field sampling

ndash September 2012 ndash 16 ponds (pond survey)

Auburn University pond facility

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf

Common toxin analytical approaches

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Common toxin analytical approaches

Outlinebull New HABs monitoring tools

bull CellScope

bull Phycocyanin

bull qPCR

bull Environmental Sample Processor (ESP)

bull Flow cytobots

bull Toxin test strips and tube kits

bull Real-time databull Forecasting websites

Outlinebull New HABs monitoring tools

httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf

Seltenrich 2014

CellScope

bull httpcellscopeberkeleyedu

Phycocyanin approaches

Turner Designs Trilogy

Orange module630nm excitation660nm emission

Field sondeHydrolab minisonde

Phycocyanin benchtop method development

bull Field sampling

ndash September 2012 ndash 16 ponds (pond survey)

Auburn University pond facility

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Chorus and Bartram 1999httpappswhointirisbitstream1066542827

10419239308_engpdfua=1

Common toxin analytical approaches

Outlinebull New HABs monitoring tools

bull CellScope

bull Phycocyanin

bull qPCR

bull Environmental Sample Processor (ESP)

bull Flow cytobots

bull Toxin test strips and tube kits

bull Real-time databull Forecasting websites

Outlinebull New HABs monitoring tools

httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf

Seltenrich 2014

CellScope

bull httpcellscopeberkeleyedu

Phycocyanin approaches

Turner Designs Trilogy

Orange module630nm excitation660nm emission

Field sondeHydrolab minisonde

Phycocyanin benchtop method development

bull Field sampling

ndash September 2012 ndash 16 ponds (pond survey)

Auburn University pond facility

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Outlinebull New HABs monitoring tools

bull CellScope

bull Phycocyanin

bull qPCR

bull Environmental Sample Processor (ESP)

bull Flow cytobots

bull Toxin test strips and tube kits

bull Real-time databull Forecasting websites

Outlinebull New HABs monitoring tools

httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf

Seltenrich 2014

CellScope

bull httpcellscopeberkeleyedu

Phycocyanin approaches

Turner Designs Trilogy

Orange module630nm excitation660nm emission

Field sondeHydrolab minisonde

Phycocyanin benchtop method development

bull Field sampling

ndash September 2012 ndash 16 ponds (pond survey)

Auburn University pond facility

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Outlinebull New HABs monitoring tools

httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf

Seltenrich 2014

CellScope

bull httpcellscopeberkeleyedu

Phycocyanin approaches

Turner Designs Trilogy

Orange module630nm excitation660nm emission

Field sondeHydrolab minisonde

Phycocyanin benchtop method development

bull Field sampling

ndash September 2012 ndash 16 ponds (pond survey)

Auburn University pond facility

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

CellScope

bull httpcellscopeberkeleyedu

Phycocyanin approaches

Turner Designs Trilogy

Orange module630nm excitation660nm emission

Field sondeHydrolab minisonde

Phycocyanin benchtop method development

bull Field sampling

ndash September 2012 ndash 16 ponds (pond survey)

Auburn University pond facility

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Phycocyanin approaches

Turner Designs Trilogy

Orange module630nm excitation660nm emission

Field sondeHydrolab minisonde

Phycocyanin benchtop method development

bull Field sampling

ndash September 2012 ndash 16 ponds (pond survey)

Auburn University pond facility

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Phycocyanin benchtop method development

bull Field sampling

ndash September 2012 ndash 16 ponds (pond survey)

Auburn University pond facility

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Pigments vs cell densities

bull Weak to no relationships between pigments and cell densities

ndash Only statistically significant relationship was chlorophyll vs phytoplankton

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Pigments vs biovolumes

bull Strong relationships between pigments and biovolume or only pigments

ndash Large range

ndash Wide diversity of taxa

ndash Chlorophyll good predictor of phycocyanin

Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

bull qPCRbull Quantifies number of gene copies associated with taxa-

and trait-specific primers and probes

bull Phytoxigenebull CyanoDTec ndash microcystin nodularin

cylindrospermopsin saxitoxin

bull DinoDTec ndash saxitoxin

bull httpwwwphytoxigenecom

bull httpswwwyoutubecomwatchv=o2bYaY1TDLs

qPCR assays

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa

bull Remotely shares data to improve forecasting models

bull httpwwwnwfscnoaagovresearchdatatechtechespcfm

Environmental Sample Processor (ESP)

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Flow cytobot

bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology

bull Remotely shares data to improve forecasting models

bull httpwwwwhoiedumainimaging-flow-cytobot

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Toxin test strips and tube kitsbull Available for several compounds including

microcystin cylindrospermopsin and anatoxin

bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins

bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome

Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Toxin test strips and tube kits

bull 2007 EPA National Lakes Assessment

bull 1252 samples for microcystin

bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL

bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys

Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Real-time data and forecastingbull Remote sensing

bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf

Stumpf et al 2015 talk

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast

System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis

bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Real-time data and forecastingbull National Centers for Coastal Ocean Sciences

(NCCOS) funding research in support of pilot regional HAB forecasts inhellip

bull Pacific Northwest - Pseudo-nitzscha

bull Puget Sound - Alexandrium

bull Gulf of Maine - Alexandrium

bull Southern California - Pseudo-nitzschia

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Real-time data and forecastingbull Great Lakes Observing System (GLOS)

bull Western Lake Erie

bull httphabsglosusmap

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie HABs tracker (forecast days 1-5)

bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)

bull Western Lake Erie

bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Real-time data and forecastingbull Phytoplankton monitoring network

bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region

bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Real-time data and forecastingbull What about inland lakes

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Real-time data and forecastingbull California Surface Water Ambient Monitoring

Program (SWAMP)bull Uses remote sensing surface water color satellite data to

estimate and forecast HABs in fresh waterbodies

bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Real-time data and forecastingbull USEPA NOAA USGS and NASA working together

to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to

estimate and forecast HABs

bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-

4753-b888-79864899c6d6

Mattas-Curry et al 2015 LakeLine

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcombloom_network

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

SECCHI DEPTH FORECASTING MODEL

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Real-time data and forecasting

httpwilsonlabcomforecastinghtml

WATER QUALITY FORECASTING MODEL

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Aquaculture pondAlabama August 2008

Contact informationAlan Wilson

Fisheries ndash Auburn University

203 Swingle Hall Auburn AL 36849

wilsonauburnedu 334-246-1120

httpwilsonlabcom

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

PAPERS

Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf

Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten

Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf

Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf

Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract

Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6

Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164

Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206

USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents

Relevant publications and links

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml

Relevant publications and linksTOOLS

CellScope httpcellscopeberkeleyedu

Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge

Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm

Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot

Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Phytoxigene httpwwwphytoxigenecom

Toxin strips httpwwwabraxiskitscomproductsalgal-toxins

Toxin tube kits httpwwwbeaconkitscomwelcome

REAL-TIME DATA AND FORECASTING

California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes

Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml

Great Lakes Observing System (GLOS) httphabsglosusmap

National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148

NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab

Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en

Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml