1. My Dog is Broken! A Presentation by Robert and Lauren

2. Background Information Champion Yorkshire Terrior Will not perform Erectile Dysfunction 3. The Process First Messenger Nitric Oxide Starts signal transduction in smooth muscle cell Second messenger cGMP Activates PKG Cellular response Relaxation Allows blood to flow, causing erection 4. The Process (continued) Arrows from smaller to bigger The response is being more amplified Guanylyl Cyclase Receptor that starts the cell signaling pathway Converts GTP to cGMP May be located in plasma membrane or in the cytoplasm 5. Effects of Ginseng Produces more nitric oxide This is required to start the process The more NO produced, the more guanylyl cyclase More cGMP More PKG 6. Effects of Viagra Inhibits PDE5 PDE5 breaks down cGMP cGMP is required to cause relaxation No relaxation, no erection 7. The Pathway This diagram helps shows the effects the two chemicals have on the pathway PKG when activated inhibits the intake of Ca2+ which triggers relaxation This is done by closing the Ca2+ channel PKG triggers reduction of Ca2+ in the cytoplasm By exporting Ca2+ and by activating Phospholamban If phospholamban is activated it cannot inhibit the calcium pumps that pump Ca2+ into the SR PKG also triggers MLCP'tase which turns MLC-P to MLC which also helps trigger relaxation 8. The Pathway (continued) 9. What if both don't work? Must be another protein that's broken Could be PKG or Phospho-proteins Or anything after cGMP in the response 10. What if both don't work? Must be another protein that's broken Could be PKG or Phospho-proteins Or anything after cGMP in the response